CN105852133A - Vitamin B microcapsule and preparation method thereof - Google Patents
Vitamin B microcapsule and preparation method thereof Download PDFInfo
- Publication number
- CN105852133A CN105852133A CN201610468520.4A CN201610468520A CN105852133A CN 105852133 A CN105852133 A CN 105852133A CN 201610468520 A CN201610468520 A CN 201610468520A CN 105852133 A CN105852133 A CN 105852133A
- Authority
- CN
- China
- Prior art keywords
- vitamin
- microcapsule
- preparation
- riboflavin
- thiamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000019156 vitamin B Nutrition 0.000 title claims abstract description 32
- 239000011720 vitamin B Substances 0.000 title claims abstract description 32
- 229930003270 Vitamin B Natural products 0.000 title claims abstract description 29
- 239000003094 microcapsule Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims abstract description 34
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 34
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims abstract description 17
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims abstract description 17
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229960000304 folic acid Drugs 0.000 claims abstract description 17
- 235000019152 folic acid Nutrition 0.000 claims abstract description 17
- 239000011724 folic acid Substances 0.000 claims abstract description 17
- 229960002477 riboflavin Drugs 0.000 claims abstract description 17
- 235000019192 riboflavin Nutrition 0.000 claims abstract description 17
- 239000002151 riboflavin Substances 0.000 claims abstract description 17
- 235000019157 thiamine Nutrition 0.000 claims abstract description 17
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229960003495 thiamine Drugs 0.000 claims abstract description 17
- 239000011721 thiamine Substances 0.000 claims abstract description 17
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 16
- 108010010803 Gelatin Proteins 0.000 claims abstract description 16
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 16
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims abstract description 16
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims abstract description 16
- 239000008273 gelatin Substances 0.000 claims abstract description 16
- 229920000159 gelatin Polymers 0.000 claims abstract description 16
- 235000019322 gelatine Nutrition 0.000 claims abstract description 16
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 16
- 239000011162 core material Substances 0.000 claims description 17
- 239000006185 dispersion Substances 0.000 claims description 12
- 239000004530 micro-emulsion Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 abstract description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 abstract 2
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 230000035764 nutrition Effects 0.000 abstract 1
- 229940088594 vitamin Drugs 0.000 description 8
- 229930003231 vitamin Natural products 0.000 description 8
- 235000013343 vitamin Nutrition 0.000 description 8
- 239000011782 vitamin Substances 0.000 description 8
- 150000003722 vitamin derivatives Chemical class 0.000 description 7
- 238000001035 drying Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000265 homogenisation Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 235000011868 grain product Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000008542 thermal sensitivity Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- -1 with nicotiamide Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a vitamin B microcapsule, having a core made from nicotinamide, thiamine, riboflavin and folic acid and a wall made from carboxymethyl cellulose and gelatin. The invention also provides a preparation method of the vitamin B microcapsule. The nicotinamide, thiamine, riboflavin and folic acid highly prone to absence in cereals are made into the core, and the prepared microcapsule may be added to cereals to strength nutrition; the carboxymethyl cellulose and gelatin are made into the wall, embedding rate of vitamin B is high, and stability of the core in the absence of oxygen under high humidity and temperature can be effectively improved.
Description
Technical field
The invention belongs to food technology field, particularly relate to a kind of vitamin B group microcapsule and preparation method thereof.
Background technology
Most cereal product lacks vitamin B group, and adding vitamin in most grain is that simple physics adds, weak effect, and loss is serious, affect grain quality simultaneously, so vitamin B group is often embedded by present stage, make microcapsule, it is then added among grain, and conventional research mostly all is the embedding of single vitamin and more with fatsoluble vitamin embedding, less for water soluble vitamins research, and end result embedding less effective, can not largely improve the vitamin B group content of grain.
Summary of the invention
In view of this, it is an object of the invention to provide a kind of vitamin B group microcapsule and preparation method thereof, the vitamin B group embedding rate that the present invention provides is higher, adds the nutritive value that can improve grain in cereal product to.
The invention provides a kind of vitamin B group microcapsule, with nicotiamide, thiamine, riboflavin and folic acid as core, with carboxymethyl cellulose and gelatin for wall material.
The present invention, with the nicotiamide, thiamine, folic acid and the riboflavin that easily lack in grain as core, prepares the effect that can play fortification after microcapsule adds in grain；The present invention, using carboxymethyl cellulose and gelatin as wall material, has higher embedding rate during embedding said vitamin B, it is possible to be effectively improved core stability under aerobic, high humidity and hot conditions.
In the present invention, the mass ratio of described nicotiamide, thiamine, riboflavin and folic acid is 20:2:2:1.The mass ratio of described carboxymethyl cellulose and gelatin is 1:1.The embedding rate of described microcapsule is 88% ~ 92%.
The present invention chooses the vitamin B group easily lacked in four kinds of grains: " nicotiamide, thiamine, riboflavin, folic acid ", added by the proportioning of science and carry out micro encapsulation test, the embedding effect of organic wall material, overcome the oxidizable rotten unfavorable factor during processing and storage of the material with thermal sensitivity, heliosensitivity on largely, the embedding rate of microcapsule is greatly improved.The present invention utilizes gelatin and carboxymethyl cellulose that B vitamin is carried out Bao Li, further optimization has been carried out simultaneously for embedding process, thus it being avoided as much as the oxidational losses of vitamin B group, the enteric solubility of wall material controls the release of vitamin B group the most orientablely simultaneously.The selection of vitamin B group kind and the determination of proportional quantity in the present invention, it is ensured that the science of grain fortification and safety.
Present invention also offers the preparation method of a kind of vitamin B group microcapsule, comprise the following steps:
A) riboflavin, thiamine, folic acid and nicotiamide are dissolved, obtain core material solution；
B) described core material solution is joined in cmc soln, after dispersion, obtain microemulsion；
C) described microemulsion is joined in gelatin solution, carry out vacuum lyophilization after dispersion, homogenizing, obtain vitamin B group microcapsule.
The present invention, first by riboflavin heating for dissolving under 80 DEG C of water bath condition, adds thiamine, folic acid and nicotiamide, obtains core material solution after cooling.Wherein, the mass ratio of nicotiamide, thiamine, riboflavin and folic acid is 20:2:2:1.
After 80 DEG C of heating in water bath of cmc soln, increase its mobility, reduce viscosity, after then core material solution being added thereto dispersion, obtain microemulsion.After mixing, the gross mass of described riboflavin, thiamine, folic acid and nicotiamide and the mass ratio of carboxymethyl cellulose are 2:2 ~ 3, preferably 2:2.73, and solution concentration is 2%.Described dispersion uses high-speed homogenization machine to carry out, and rotating speed is 10000rpm, and jitter time is 30s, is repeated 3 times, and uses magnetic stirrer to stir, fully dispersed.
After obtaining microemulsion, adding it to disperse in gelatin solution, described dispersion uses high-speed homogenization machine to carry out, and rotating speed is 20000rpm, and jitter time is 30s, and temperature is 50 DEG C.Then high pressure homogenize under 30 ~ 50MPa, in triplicate.The mass ratio of gelatin and carboxymethyl cellulose is 1:1.
Finally solution being carried out vacuum lyophilization, product pulverizes and sieves, and i.e. can get vitamin B group microcapsule.Its embedding rate is 88 ~ 92%.
The present invention is directed to the shortcomings such as existing composite B vitamin microcapsule embedded technical stability difference embedding rate is low, use and there is the carboxymethyl cellulose of high stability and gelatin embed core vitamin as wall material, hydrophilic embedding vitamin B group first with carboxymethyl cellulose uses the gelatin with non-oxidizability and emulsibility and enteric solubility to embed vitamin B group further again, achieve good encapsulating effect, it is effectively increased core in the sun, oxygen, stability under high humility and hot conditions, use Vacuum Freezing & Drying Technology to be dried simultaneously, effectively reduce the loss to the vitamin B group of thermal sensitivity of the existing spray drying technology, thus embedding rate is greatly improved.
Detailed description of the invention
Embodiment 1
(1) prepared by core material solution
Take 0.16g riboflavin, join in 100ml water, heating for dissolving under 80 DEG C of water bath condition, add thiamine, folic acid and nicotiamide, stirring and evenly mixing after cooling, make core material solution；Wherein, thiamine: riboflavin folic acid: nicotiamide=2 21 20 m/m.
Preparation with carboxymethyl cellulose as continuous phase, the vitamin microemulsion as dispersion phase: cmc soln is heated in 80 DEG C of water-baths, add its mobility, reduce viscosity, core is joined in cmc soln and mixes, vitamin total amount is 2:2.73 with the mass ratio of carboxymethyl cellulose in the mixture, solution concentration is 2%, solution high-speed homogenization machine 10000rpm rapid dispersion 30s, in triplicate；And use magnetic stirrer to stir so that it is fully mix.
(2) being distributed to the microemulsion that step (1) prepares with the aqueous gelatin solution of carboxymethyl cellulose equal in quality, solution concentration is still 2%, and now the ratio of vitamin total amount and gelatin is 1:2.73.Dispersion uses 20000rpm rapid dispersion 30s, and temperature controls at 50 DEG C.Then by solution high pressure homogenize under 30-50MPa, in triplicate.
(3) micro-liquid is dried with two kinds of different drying modes of vacuum lyophilization to be spray-dried, contrasts its water content, stability, configuration of surface, select suitable drying mode.
(4) by contrast, choosing the drying mode of vacuum lyophilization, pulverized and sieved by dry products, prepare vitamin B group microcapsule, its embedding rate is 88 ~ 92 %.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a vitamin B group microcapsule, it is characterised in that with nicotiamide, thiamine, riboflavin and folic acid as core, with carboxymethyl cellulose and gelatin for wall material.
Vitamin B group microcapsule the most according to claim 1, it is characterised in that the mass ratio of described nicotiamide, thiamine, riboflavin and folic acid is 20:2:2:1.
Vitamin B group microcapsule the most according to claim 1, it is characterised in that the mass ratio of described carboxymethyl cellulose and gelatin is 1:1.
4. according to the vitamin B group microcapsule described in claim 1 ~ 3 any one, it is characterised in that the embedding rate of described microcapsule is 88% ~ 92%.
5. a preparation method for vitamin B group microcapsule, comprises the following steps:
A) riboflavin, thiamine, folic acid and nicotiamide are dissolved, obtain core material solution；
B) described core material solution is joined in cmc soln, after dispersion, obtain microemulsion；
C) described microemulsion is joined in gelatin solution, carry out vacuum lyophilization after dispersion, homogenizing, obtain vitamin B group microcapsule.
Preparation method the most according to claim 5, it is characterised in that described step a) is particularly as follows: first by riboflavin heating for dissolving under 80 DEG C of water bath condition, add thiamine, folic acid and nicotiamide, obtain core material solution after cooling.
Preparation method the most according to claim 5, it is characterised in that described step b) particularly as follows:
After 80 DEG C of heating in water bath of cmc soln, after core material solution is added thereto dispersion, obtain microemulsion.
Preparation method the most according to claim 7, it is characterised in that in described step b), the gross mass of described riboflavin, thiamine, folic acid and nicotiamide and the mass ratio of carboxymethyl cellulose are 2:2 ~ 3；Described scattered rotating speed is 10000rpm, and jitter time is 30s.
Preparation method the most according to claim 5, it is characterised in that in described step c), described scattered rotating speed is 20000rpm, and jitter time is 30s.
Preparation method the most according to claim 9, it is characterised in that in described step c), described homogenizing is high pressure homogenize, and pressure is 30MPa ~ 50MPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610468520.4A CN105852133A (en) | 2016-06-25 | 2016-06-25 | Vitamin B microcapsule and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610468520.4A CN105852133A (en) | 2016-06-25 | 2016-06-25 | Vitamin B microcapsule and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105852133A true CN105852133A (en) | 2016-08-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610468520.4A Pending CN105852133A (en) | 2016-06-25 | 2016-06-25 | Vitamin B microcapsule and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105852133A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109938353A (en) * | 2019-04-26 | 2019-06-28 | 河南柏思芙德食品有限公司 | A kind of vitamin microcapsule and preparation method thereof |
| CN112006171A (en) * | 2020-09-03 | 2020-12-01 | 安徽东方天合生物技术有限责任公司 | Novel rumen-bypass compound vitamin coating process |
| CN112006172A (en) * | 2020-09-03 | 2020-12-01 | 北京东方天合过瘤胃技术研究院有限公司 | Novel rumen-bypass pantothenic acid coating process |
| CN114190507A (en) * | 2021-12-21 | 2022-03-18 | 中国热带农业科学院农产品加工研究所 | Method for preparing essential oil microcapsules by taking cassava starch alcohol-free esterification mixture as wall material |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391898A (en) * | 2002-07-25 | 2003-01-22 | 无锡健特药业有限公司 | Water soluble vitamin product coated with micro capsule and its producing method |
| CN1927202A (en) * | 2006-08-25 | 2007-03-14 | 西南合成制药股份有限公司 | Vitamin E nicotinate microcapsule and its preparing process |
| CN101194897A (en) * | 2006-12-04 | 2008-06-11 | 东北农业大学 | Method for preparing water-solubility compound vitamin microcapsule |
| CN101947214A (en) * | 2010-07-17 | 2011-01-19 | 郭善广 | Method for preparing vitamin A microcapsules by secondary emulsification and spray drying method |
| CN102106480A (en) * | 2011-01-26 | 2011-06-29 | 广东省农业科学院畜牧研究所 | B-complex crystal vitamin microcapsule and method for preparing microcapsules by utilizing spray drying method |
| CN103013969A (en) * | 2011-09-20 | 2013-04-03 | 哈尔滨商业大学 | Microencapsulated protease preparation method |
-
2016
- 2016-06-25 CN CN201610468520.4A patent/CN105852133A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1391898A (en) * | 2002-07-25 | 2003-01-22 | 无锡健特药业有限公司 | Water soluble vitamin product coated with micro capsule and its producing method |
| CN1927202A (en) * | 2006-08-25 | 2007-03-14 | 西南合成制药股份有限公司 | Vitamin E nicotinate microcapsule and its preparing process |
| CN101194897A (en) * | 2006-12-04 | 2008-06-11 | 东北农业大学 | Method for preparing water-solubility compound vitamin microcapsule |
| CN101947214A (en) * | 2010-07-17 | 2011-01-19 | 郭善广 | Method for preparing vitamin A microcapsules by secondary emulsification and spray drying method |
| CN102106480A (en) * | 2011-01-26 | 2011-06-29 | 广东省农业科学院畜牧研究所 | B-complex crystal vitamin microcapsule and method for preparing microcapsules by utilizing spray drying method |
| CN103013969A (en) * | 2011-09-20 | 2013-04-03 | 哈尔滨商业大学 | Microencapsulated protease preparation method |
Non-Patent Citations (2)
| Title |
|---|
| 范青生: "《保健食品工艺学》", 30 November 2006, 中国医药科技出版社 * |
| 谢岩黎: "《现代食品工程技术》", 31 May 2011, 郑州大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109938353A (en) * | 2019-04-26 | 2019-06-28 | 河南柏思芙德食品有限公司 | A kind of vitamin microcapsule and preparation method thereof |
| CN112006171A (en) * | 2020-09-03 | 2020-12-01 | 安徽东方天合生物技术有限责任公司 | Novel rumen-bypass compound vitamin coating process |
| CN112006172A (en) * | 2020-09-03 | 2020-12-01 | 北京东方天合过瘤胃技术研究院有限公司 | Novel rumen-bypass pantothenic acid coating process |
| CN114190507A (en) * | 2021-12-21 | 2022-03-18 | 中国热带农业科学院农产品加工研究所 | Method for preparing essential oil microcapsules by taking cassava starch alcohol-free esterification mixture as wall material |
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Application publication date: 20160817 |
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