CN105851455A - Preparation method of high-immune plasma protein powder - Google Patents
Preparation method of high-immune plasma protein powder Download PDFInfo
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- CN105851455A CN105851455A CN201610230019.4A CN201610230019A CN105851455A CN 105851455 A CN105851455 A CN 105851455A CN 201610230019 A CN201610230019 A CN 201610230019A CN 105851455 A CN105851455 A CN 105851455A
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- albumen powder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a preparation method of high-immune plasma protein powder. According to the method, fresh plasma liquid of healthy slaughtered livestock and poultry as a raw material, a process method including the steps of inorganic membrane filtering, organic membrane concentrating, reducing sugar adding, high-pressure homogenizing and spray drying is adopted, the prepared high-immune plasma protein powder basically has no blood smell, the protein content of the high-immune plasma protein powder reaches 75% or above, and the immune globulin G content of the high-immune plasma protein powder is increased by 40% or above compared with common plasma protein powder; reducing sugar and plasma immune globulin particles are prepared through reducing sugar adding, homogenizing and centrifugal atomization treating, damage of the high temperature to immune globulin in the spray drying process is reduced through the affinity effect of rich hydrophilic hydroxyl groups in reducing sugar to water, therefore, the activity of the immune globulin is guaranteed, and preparation of the high-immune plasma protein powder is achieved.
Description
Technical field
The invention belongs to livestock and poultry blood processing and utilize technical field, being specifically related to the preparation of a kind of highly immunized plasma albumen powder
Method.
Background technology
China is that live pig produces the first big country in the world, causes death or eliminate to reach due to a variety of causes such as diseases every year
Several ten million heads, wherein within 30kg, piglet is eliminated death toll and accounts for whole finishing period and eliminate more than half of the death rate, causes this
The main cause of phenomenon is exactly that piglet Abwehrkraft des Koepers is weak, the incidence of disease is high, cause piglet survival ratio reduction, the price of deed to reduce,
Poor growth, stagnation (cad pig) of growing, the sound development of serious threat pig industry, China is every year to these special rank of piglet
The raising of section, management put into over ten billion Yuan with child care.Spray-dried blood plasma albumen powder is the increase that the effect reported at present is best
The biological product of cub Abwehrkraft des Koepers, therefore, can not suspect that SDPP is before this market just has wide market
Scape.
On existing market, spray-dried blood plasma albumen powder is all with healthy fresh pig blood as raw material, fresh-keeping through anti-freezing,
Centrifugation, the ultrafiltration concentration technique such as desalination, spray drying are prepared from, and existing research report points out function in SDPP
Property albumen--immunoglobulin G (IgG) is the nucleus of its function, IgG is a kind of antibody protein, can rise with exogenous antigen
Immune response, generates antigen-antibody complex, thus blocks most of bacterium, the virus harm to body.Directly make an addition to son
In pig feed, can be effectively improved immunity of organisms, pre-anti-diarrhea, improve piglet survival ratio, cooperation antibiotic can be pre-simultaneously
Prevent and treat the multiple diseases such as swine flu, transmissible gastroenteritis, epidemic diarrhea, necrotic enteritis, it is possible to significantly reducing antibiosis
The application of the medicines such as element.
Owing to immunoglobulin G is a kind of thermal sensitivity albumen, process is highly prone to the impact of external heat, and lives
Property sustain damage, have research to point out in the solid state, at a temperature of 120 DEG C expose 60 seconds, damage is surpassed 50% by its immunocompetence,
Its extent of damage is more serious in the solution.But high temperature spray-drying operation in existing plasma protein flour producing process, due to
It can realize moisture in plasma solutions and the most in a disguised form evaporate, and high temperature kills effect of various sex pheromones in blood plasma, because of
And be difficult to be replaced, owing to livestock and poultry fresh separated blood plasma solid content is relatively low, about at 6-8%, the most concentrated Direct spraying is done
Dry, the highest inlet temperature is even up to 240 DEG C, has immunocompetent Lysozyme impaired in dry run in plasma protein
Seriously, product biological value is reduced.Although using desivac can prepare high activity SDPP, but efficiency being low,
Energy consumption is big, and the sex pheromone in blood is also difficult to kill, therefore, IgG antihunt means in research effective plasma level albumen, exploitation height
Exempt from SDPP and there is significant application value.
Summary of the invention
In order to improve existing animal blood plasma albumen technological method for processing, reduce blood plasma liquid at high-temperature drying procedures to immunity
The damage of the activity of globulin, improves immunoglobulin content in SDPP, and the present invention provides a kind of highly immunized plasma albumen
The preparation method of powder.
The preparation manipulation step of a kind of highly immunized plasma albumen powder is as follows:
A. take the animal blood slurries of fresh separated, animal blood slurries are crossed 150 mesh filter bags, pump into the inoranic membrane that aperture is 0.22 μm
Filtration system, it is 10000～20000D organic film concentration systems that filtrate pumps into molecular cut off again, circulation concentrating and desalinating dehydration,
Obtain solid content and reach the deshydremia slurries of 16-20%；
B. deshydremia slurries are pumped into the accumulator tanks equipped with agitator, according to deshydremia slurries total amount mass percent 1.0～
3.0% adds reduced sugar, 30～45 DEG C, under the conditions of rotating speed is 120～200 rpm, stirring and dissolving 15～30min, obtain sugaring
Blood plasma liquid；
C. sugaring blood plasma liquid pump is entered homogenizer, under the conditions of pressure 10～30MPa, high-pressure homogeneous 2～3 times, obtain homogeneous and add
Glucemia slurries；
D. by homogeneous sugaring blood plasma liquid EAT 170～200 DEG C, it is spray-dried under the conditions of leaving air temp 70～100 DEG C,
Obtain highly immunized plasma albumen powder；
Described highly immunized plasma albumen powder, good fluidity, bloody lightly seasoned, wherein protein content reaches more than 75%, with normal plasma egg
White powder is compared, and its immunoglobulin content improves more than 40%.
The technical scheme limited further is as follows:
Described fresh animal blood slurries are Swine plasma liquid, ox blood slurries, chicken plasma liquid.
Described reduced sugar is at least two kinds in glucose, fructose, maltose and maltodextrin.
When using two kinds of reduced sugars, the mass ratio of two kinds of reduced sugars is 3:1.
When using three kinds of reduced sugars, the mass ratio of three kinds of reduced sugars is 2～8:1:1.
The Advantageous Effects of the present invention is embodied in the following aspects:
1. the present invention is directed to be spray-dried high temperature to the problem of immunoglobulin (Ig) damage in blood plasma, the modern bio-chemistry separation of employing and merit
The protein stabilized technology of energy, is proposed a kind of stabilisation by filtration sterilization and protein glycosylation and improves functional protein at spray drying
Stability in hot conditions.Survey through enzyme linked immunological biopsy, prepared highly immunized plasma albumen powder and normal plasma albumen powder phase
Ratio, immunoglobulin G content improves 40%, simultaneously because with the addition of reduced sugar and make SDPP substantially without fishy smell, whole
Improve product quality on body, enhance product functionality.
2. have employed inorganic membrane filtration in the present invention and organic film concentration technique combines, wherein inorganic membrane filtration can be by
In animal blood slurries, microorganism carries out isolation filtration, makes up spray drying temperature and declines the bactericidal effect impact brought, tries hard to keep
Card product biological safety, and the organic film used can effectively deviate from moisture and salinity in blood plasma, reduces the ash of SDPP
Point, increase percent protein.
3.. present invention process is by adding reduced sugar, homogenizing and be spray-dried, and lives with immunoglobulin (Ig) in stable blood plasma
Property.The reduced sugar added has excellent water solubility and great amount of hydroxy group, can be with immunoglobulin (Ig) phase mutual connection in blood plasma after homogeneous
Touching, formed microgranular when centrifugal atomizing with immunoglobulin (Ig) in blood plasma, great amount of hydroxy group has good hydrophily, it is possible at mist
Change in particulate flash drying process, stably keep moisture content, thus reduce immunoglobulin molecules Contact Temperature, serve stable exempting from
The effect of epidemic disease globulin functional activity.It addition, the albumen in reduced sugar and blood plasma, glycans can occur under the high temperature conditions
Change reaction, also be able to after protein glycosylation increase molecular weight of albumen, improve its stability.
Detailed description of the invention
Below by specific embodiment, the invention will be further described.
Embodiment 1:
Raw material selects: the Swine plasma liquid that healthy live pig is fresh；
A. take the Swine plasma liquid of fresh separated, Swine plasma liquid is crossed 150 mesh filter bags, pump into the inorganic membrane filtration that aperture is 0.22 μm
System, it is 10000D organic film concentration systems that filtrate pumps into molecular cut off again, circulation concentrating and desalinating dehydration, obtains solid content and contains
Amount reaches the deshydremia slurries of 18%；
B. deshydremia slurries are pumped into the accumulator tanks equipped with agitator, add reduction according to the mass percent 2.0% of total plasma volume
Sugar, added reduced sugar be maltodextrin sugar and glucose, its mass ratio is 3:1, then 40 DEG C, rotating speed be 180rpm condition
Under, stirring and dissolving 25min, obtain sugaring blood plasma liquid；
C. sugaring blood plasma liquid pump is entered homogenizer, under the conditions of pressure 20MPa, high-pressure homogeneous 3 times, obtain homogeneous sugaring blood plasma
Liquid；
D. by homogeneous sugaring blood plasma liquid EAT 190 DEG C, it is spray-dried under the conditions of leaving air temp 90～100 DEG C, obtains high
Exempt from Swine plasma protein；
Quality Detection:
1. outward appearance detection:
Sample is placed on clean ceramic whiteware dish, visual, nasil under natural light；
2. protein detection:
Mensuration according to GB 5009.5-2010 national food safety standard Protein in Food；
3. carbohydrate detection:
According to the mensuration of reduced sugar in GB/T 5009.7-2008 national food safety standard food；
4. ash content detection:
According to the mensuration of ash content in GB 5009.4-2010 national food safety standard food；
5. water content detection:
According to the mensuration of moisture in GB 5009.3-2010 national food safety standard food；
5. immunoglobulin G assay:
Use the detection of PIG detection kit；
Concrete testing result is shown in Table 1.
From table 1, using the highly immunized plasma albumen powder that the inventive method produces, good fluidity, bloody lightly seasoned, albumen contains
Amount reaches 75.4%, and compared with normal plasma albumen powder, its immunoglobulin content significantly improves, and wherein epidemic disease Lysozyme content reaches
21.2%。
Common identical with the quality detecting method of highly immunized plasma albumen powder.
Embodiment 2:
Raw material selects: the blood plasma liquid that healthy ox is fresh
A. take the ox blood slurries of fresh separated, ox blood slurries are crossed 150 mesh filter bags, pump into the inorganic membrane filtration that aperture is 0.22 μm
System, it is 20000D organic film concentration systems that filtrate pumps into molecular cut off again, circulation concentrating and desalinating dehydration, obtains solid content and contains
Amount reaches the deshydremia slurries of 20%；
B. deshydremia slurries are pumped into the accumulator tanks equipped with agitator, add reduction according to the mass percent 3.0% of total plasma volume
Sugar, added reduced sugar is maltodextrin, glucose and fructose, and its mass ratio is 2:1:1, then 45 DEG C, rotating speed be
Under the conditions of 200rpm, stirring and dissolving 30min, obtain sugaring blood plasma liquid；
C. sugaring blood plasma liquid pump is entered homogenizer, under the conditions of pressure 30MPa, high-pressure homogeneous 2 times, obtain homogeneous sugaring blood plasma
Liquid；
D. by homogeneous sugaring blood plasma liquid EAT 170 DEG C, it is spray-dried under the conditions of leaving air temp 70～80 DEG C, obtains high
Exempt from ox blood slurry protein powder；
Detection method is in addition to immunoglobulin G uses the detection of cattle immune globulin G detection kit, and other are ibid.
Concrete testing result is shown in Table 2.
From table 2, using the highly immunized plasma albumen powder that the inventive method produces, good fluidity, bloody lightly seasoned, albumen contains
Amount reaches 75.1%, and compared with normal plasma albumen powder, its immunoglobulin content significantly improves, and wherein epidemic disease Lysozyme content reaches
24.7%。
Common identical with the quality detecting method of highly immunized plasma albumen powder.
Embodiment 3:
Raw material selects: the blood plasma liquid that healthy chicken is fresh
A. take the chicken plasma liquid of fresh separated, chicken plasma liquid is crossed 150 mesh filter bags, pump into the inorganic membrane filtration that aperture is 0.22 μm
System, it is 10000D organic film concentration systems that filtrate pumps into molecular cut off again, circulation concentrating and desalinating dehydration, obtains solid content and contains
Amount reaches the deshydremia slurries of 16%；
B. deshydremia slurries are pumped into the accumulator tanks equipped with agitator, add reduction according to the mass percent 1.0% of total plasma volume
Sugar, added reduced sugar is maltodextrin, glucose and maltose, and its mass ratio is 8:1:1, then 30 DEG C, rotating speed be
Under the conditions of 120rpm, stirring and dissolving 15min, obtain sugaring blood plasma liquid；
C. sugaring blood plasma liquid pump is entered homogenizer, under the conditions of pressure 10MPa, high-pressure homogeneous 3 times, obtain homogeneous sugaring blood plasma
Liquid；
D. by homogeneous sugaring blood plasma liquid EAT 200 DEG C, it is spray-dried under the conditions of leaving air temp 80～90 DEG C, obtains high
Exempt from chicken plasma albumen powder；
Detection method is in addition to immunoglobulin G uses the detection of Chicken immunoglobulin G detection kit, and other are ibid.
Concrete testing result is shown in Table 3.
From table 3, using the highly immunized plasma albumen powder that the inventive method produces, good fluidity, bloody lightly seasoned, albumen contains
Amount reaches 77.3%, and compared with normal plasma albumen powder, its immunoglobulin content significantly improves, and wherein epidemic disease Lysozyme content reaches
17.5%。
Common identical with the quality detecting method of highly immunized plasma albumen powder.
Claims (5)
1. the preparation method of a highly immunized plasma albumen powder, it is characterised in that comprise the steps:
A. take the animal blood slurries of fresh separated, animal blood slurries are crossed 150 mesh filter bags, pump into the inoranic membrane that aperture is 0.22 μm
Filtration system, it is 10000～20000D organic film concentration systems that filtrate pumps into molecular cut off again, circulation concentrating and desalinating dehydration,
Obtain solid content and reach the deshydremia slurries of 16-20%；
B. deshydremia slurries are pumped into the accumulator tanks equipped with agitator, according to deshydremia slurries total amount mass percent 1.0～
3.0% adds reduced sugar, 30～45 DEG C, under the conditions of rotating speed is 120～200rpm, stirring and dissolving 15～30min, obtain sugaring
Blood plasma liquid；
C. sugaring blood plasma liquid pump is entered homogenizer, under the conditions of pressure 10～30MPa, high-pressure homogeneous 2～3 times, obtain homogeneous and add
Glucemia slurries；
D. by homogeneous sugaring blood plasma liquid EAT 170～200 DEG C, it is spray-dried under the conditions of leaving air temp 70～100 DEG C,
Obtain highly immunized plasma albumen powder；
Described highly immunized plasma albumen powder, good fluidity, bloody lightly seasoned, wherein protein content reaches more than 75%, with normal plasma egg
White powder is compared, and its immunoglobulin content improves more than 40%.
The preparation method of a kind of highly immunized plasma albumen powder the most according to claim 1, it is characterised in that: described fresh poultry
Fowl blood plasma liquid is Swine plasma liquid, ox blood slurries, chicken plasma liquid.
The preparation method of a kind of highly immunized plasma albumen powder the most according to claim 1, it is characterised in that: described reduced sugar is
In glucose, fructose, maltose and maltodextrin at least two kinds.
The preparation method of a kind of highly immunized plasma albumen powder the most according to claim 3, it is characterised in that: use two kinds of reduction
During sugar, the mass ratio of two kinds of reduced sugars is 3:1.
The preparation method of a kind of highly immunized plasma albumen powder the most according to claim 3, it is characterised in that: use three kinds of reduction
During sugar, the mass ratio of three kinds of reduced sugars is 2～8:1:1.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2015108090104 | 2015-11-20 | ||
| CN201510809010 | 2015-11-20 |
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| CN105851455A true CN105851455A (en) | 2016-08-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610230019.4A Pending CN105851455A (en) | 2015-11-20 | 2016-04-13 | Preparation method of high-immune plasma protein powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866810A (en) * | 2017-04-01 | 2017-06-20 | 合肥禹王膜工程技术有限公司 | Low ash content plasma protein tubular membrane concentration technology |
| CN108813090A (en) * | 2018-06-06 | 2018-11-16 | 江苏省农业科学院 | The preparation method of poultry plasma protein |
| CN110692801A (en) * | 2019-11-14 | 2020-01-17 | 合肥工业大学 | Reconstituted plasma protein powder and preparation method thereof |
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| CN1939537A (en) * | 2005-09-27 | 2007-04-04 | 程国根 | Production of gamma immune protein capsules |
| CN102010459A (en) * | 2010-09-10 | 2011-04-13 | 合肥工业大学 | Method for preparing animal blood plasma albumen powder |
| CN102318722A (en) * | 2011-06-15 | 2012-01-18 | 天津宝迪农业科技股份有限公司 | Method for preparing high-protein food-grade plasma protein powder |
| CN103039693A (en) * | 2013-01-09 | 2013-04-17 | 合肥工业大学 | Preparation method of modified livestock and poultry plasma protein powder |
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2016
- 2016-04-13 CN CN201610230019.4A patent/CN105851455A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1939537A (en) * | 2005-09-27 | 2007-04-04 | 程国根 | Production of gamma immune protein capsules |
| CN102010459A (en) * | 2010-09-10 | 2011-04-13 | 合肥工业大学 | Method for preparing animal blood plasma albumen powder |
| CN102318722A (en) * | 2011-06-15 | 2012-01-18 | 天津宝迪农业科技股份有限公司 | Method for preparing high-protein food-grade plasma protein powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866810A (en) * | 2017-04-01 | 2017-06-20 | 合肥禹王膜工程技术有限公司 | Low ash content plasma protein tubular membrane concentration technology |
| CN106866810B (en) * | 2017-04-01 | 2020-07-31 | 合肥禹王膜工程技术有限公司 | Tubular membrane concentration process for low-ash plasma protein |
| CN108813090A (en) * | 2018-06-06 | 2018-11-16 | 江苏省农业科学院 | The preparation method of poultry plasma protein |
| CN108813090B (en) * | 2018-06-06 | 2022-03-08 | 江苏省农业科学院 | Preparation method of poultry plasma protein |
| CN110692801A (en) * | 2019-11-14 | 2020-01-17 | 合肥工业大学 | Reconstituted plasma protein powder and preparation method thereof |
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