CN105851067A - Fennel extract and preparation method thereof, and application of fennel extract in controlling growth of duckweeds - Google Patents
Fennel extract and preparation method thereof, and application of fennel extract in controlling growth of duckweeds Download PDFInfo
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- CN105851067A CN105851067A CN201610189922.0A CN201610189922A CN105851067A CN 105851067 A CN105851067 A CN 105851067A CN 201610189922 A CN201610189922 A CN 201610189922A CN 105851067 A CN105851067 A CN 105851067A
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- fructus foeniculi
- extracting solution
- herba spirodelae
- fennel extract
- water
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a fennel extract and a preparation method thereof, and application of the fennel extract in controlling the growth of duckweeds, and relates to the field of water protection. The method comprises the following step: subjecting fresh fennel stems and leaves to soaking extraction so as to obtain the fennel extract. The fennel extract provided by the invention is adopted to control the growth of the duckweeds or kill the duckweeds, has the advantages of greenness, environmental protection, harmlessness to human and livestock, etc., and has extensive application prospects.
Description
Technical field
The present invention relates to water protection field, be more particularly to Fructus Foeniculi extracting solution and preparation method, and Fructus Foeniculi extracting solution is used for controlling the growth of Herba Spirodelae, or kill Herba Spirodelae, to protect the balance of aquatic ecological.
Background technology
Herba Spirodelae (Lemna minorL.), Arales Lemnaceae Lemna fluitante, it is distributed widely in irrigation canals and ditches all over the world, river, pond and lake.In recent years, the aggravation polluted along with nutrients such as water body nitrogen phosphorus, intensive culture brings the disappearance of Herba Spirodelae natural enemy (such as duck, Ctenopharyngodon idellus) in addition, under the conditions of good growing weather, Herba Spirodelae usually explosive growth in the even lake of some irrigation canals and ditches, river course, pond.Herba Spirodelae itself has the strongest absorption and detergent power to water body nitrogen, phosphorus and heavy metal etc., and appropriate growth can purify water, and is a kind of excellent animal feed；But as a kind of fluitante, the growth of its explosion type not only can reduce Dissolved Oxygen in Water, hinder Underwater Optical to shine, affect the growth of underwater plant, destroy original aquatic ecological balance, but also can affect the utilization of water body shipping, view and water resource.Therefore, the control of water body Herba Spirodelae is an environmental problem urgently to be resolved hurrily.
At present, water body Herba Spirodelae control method mainly has the methods such as artificial salvaging, BIOLOGICAL CONTROL and chemical herbicide control: manually salvage time and effort consuming, and the characteristic easily spread due to Herba Spirodelae itself and Herba Spirodelae is difficult to thoroughly and removes, the Herba Spirodelae of residual is broken out the most again when condition is suitable, in addition, in some hygrophyte clumps, the Herba Spirodelae on canopy type submerged plant surface, artificial salvaging is difficult to carry out.BIOLOGICAL CONTROL presently mainly utilizes duck, Ctenopharyngodon idellus etc. that the predation of Herba Spirodelae is reached the control to Herba Spirodelae, Herba Spirodelae is controlled by the method in early days may be effectively, but animal stocking again after Herba Spirodelae breaks out and causes environmental problem, due to Herba Spirodelae propagation rapidly, animal ingestion does not has control action, reduces the density effect of Herba Spirodelae the most to a certain extent；Additionally, in water body, animal may preferentially ingest, and it likes food, as duck can preferentially prey on the aquatic animal such as small fish, snail, the submerged plant etc. that Ctenopharyngodon idellus prioritizing selection is suitable.Although chemical herbicide has good killing effect to Herba Spirodelae, but the secondary of environment is endangered and can not be ignored by it.Compared with chemical herbicide, the features such as allelochemical or natural herbicide have efficiently, environmental protection, selectivity are strong, have broad application prospects.
Summary of the invention
It is an object of the invention to: a kind of Fructus Foeniculi extracting solution and preparation method are provided.It is another object of the present invention to: Fructus Foeniculi extracting solution is used for controlling the growth of Herba Spirodelae, or kills Herba Spirodelae, to protect the balance of aquatic ecological.
In order to achieve the above object, a kind of Fructus Foeniculi extracting solution of the present invention is prepared as follows, specifically comprises the following steps that
A, leaf, stem and root by fresh Fructus Foeniculi air-dry after washing；
B, air-dry after Fructus Foeniculi leaf, stem and root cut into fragment, put in container；
C, Fructus Foeniculi and water are added sterilized water according to the weight ratio of 1:10 ~ 30；
Soaking at room temperature 2 ~ 4 days under d, dark condition；
E, first Fructus Foeniculi fragment is pulled out, then with 1.2 μm acetate fiber membrane filtrations, obtain Fructus Foeniculi extracting solution；
F, Fructus Foeniculi extracting solution keep in Dark Place at 4 DEG C.
In above-mentioned steps b, the diameter of Fructus Foeniculi fragment is more preferably less than 5 mm.
Fructus Foeniculi and the preferred 1:10 of weight ratio of water in above-mentioned steps c.
Temperature when soaking in above-mentioned steps d is preferably 25 DEG C ± 2 DEG C, and soak time is 3 days.
A kind of Fructus Foeniculi extracting solution can be used for controlling the growth of Herba Spirodelae, or kills Herba Spirodelae.
Traditional chemical herbicide has good killing effect to Herba Spirodelae, but due to its risk to ecological environment Yu human health, limits its application in natural water body.Compared with traditional chemical herbicide, the Fructus Foeniculi extracting solution of the present invention controls the growth of Herba Spirodelae, or kill Herba Spirodelae there is environmental protection, to advantages such as person poultry harmless, have broad application prospects.
Accompanying drawing explanation
The Herba Spirodelae lobe number cartogram that Herba Spirodelae is controlled by a kind of Fructus Foeniculi extracting solution various dose of Fig. 1 present invention.
Control design sketch (a, the comparison to Herba Spirodelae of a kind of Fructus Foeniculi extracting solution of Fig. 2 present invention；7th day photo figure after processing containing 50 ml extracting solution in b, 200 ml culture fluid).
After Herba Spirodelae is processed 7 days by a kind of Fructus Foeniculi extracting solution various dose of Fig. 3 present invention, (a, O are schemed in the damage of Herba Spirodelae leaf oxidation2 -Generate；B, MDA generate).
A kind of Fructus Foeniculi extracting solution various dose of Fig. 4 present invention processes rear blade photosynthesis variation diagram (a, the change of photosynthetical system II electron transport rate rETR to Herba Spirodelae；B, the change of photosynthetical system II Efficiency of primary conversion of light energy Fv/Fm).
Fig. 5 sprays the Herba Spirodelae lobe number cartogram that Herba Spirodelae is controlled by a kind of Fructus Foeniculi extracting solution of the various dose present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is further described.
Embodiment one
A kind of Fructus Foeniculi extracting solution of the present invention is prepared as follows, specifically comprises the following steps that
A, leaf, stem and root by fresh Fructus Foeniculi air-dry after washing；
B, air-dry after Fructus Foeniculi leaf, stem and root cut into fragment, put in container；
C, Fructus Foeniculi and water are added sterilized water according to the weight ratio of 1:10 ~ 30；
Soaking at room temperature 2 ~ 4 days under d, dark condition；
E, first Fructus Foeniculi fragment is pulled out, then with 1.2 μm acetate fiber membrane filtrations, obtain Fructus Foeniculi extracting solution；
F, Fructus Foeniculi extracting solution keep in Dark Place at 4 DEG C.
In above-mentioned steps b, the diameter of Fructus Foeniculi fragment is more preferably less than 5mm.
Fructus Foeniculi and the preferred 1:10 of weight ratio of water in above-mentioned steps c.
Temperature when soaking in above-mentioned steps d is preferably 25 DEG C ± 2 DEG C, and soak time is 3 days.
Fructus Foeniculi extracting solution can be used for controlling the growth of Herba Spirodelae, or kills Herba Spirodelae.
Embodiment two
By be collected in Fructus Foeniculi from irrigation canals and ditches limit, Kunming Dianchi lake Fu Shan village one (Foeniculum vulgare) fresh stem and leaf, after cleaning 3-5 time with distilled water, it is positioned at room ventilation after natural air drying, is cut into the diameter fragment less than 5 mm.Put in 2 L Brown Glass Brown glass bottles and jars onlys, add water by the concentration of 100g FW/L, soak 3 days under dark condition in 25 ± 2 DEG C of incubators, rear mistake 1.2 μm filter membrane, obtaining concentration is 100 mg FW/ml vegetation water extracting solution, and is preserved at 4 DEG C in sterilizing brown reagent bottle by this extracting solution.
The experiment effect 1 of Herba Spirodelae is controlled with Fructus Foeniculi extracting solution.
Fructus Foeniculi extracting solution prepared by embodiment two be used for suppressing Herba Spirodelae (Lemna minorIt is collected in Kunming Dianchi lake Cao Hai Inst. of Hydrobiology, Chinese Academy of Sciences water special project demonstration project to enclose in cadmium) experiment that grows, specifically comprise the following steps that in the beaker of 250 ml, add the Hoagland solution of 100 ml sterilizings and the Fructus Foeniculi extracting solution of various dose, it is respectively 0,5,10,20,50 ml, and is settled to 200 ml with distilled water.Herba Spirodelae 240 lobe clean, healthy, of the same size is added to each beaker, illumination box is cultivated 8 days (temperature 25 ± 1 DEG C, light intensity 20 ~ 25 μm ol/m2/ s, Light To Dark Ratio 12/12), add the moisture of evaporation every day.Implementation process measures statistics Herba Spirodelae lobe number, oxidative damage, chlorophyll fluorescence feature etc..Parallel 3 groups of Setup Experiments.Result of implementation is as illustrated in the accompanying drawings from 1 to 3.After using this extracting solution, Herba Spirodelae blade propagation is significantly inhibited, and in 200 ml culture fluid, 5 ml extracting solution can significantly inhibit the growth of Herba Spirodelae after 1 day, and after adding 50 ml extracting solution 7 days, Herba Spirodelae is all dead (accompanying drawing 1).By measuring Herba Spirodelae blade O2 -, MDA concentration and photosynthetical system II efficiency (accompanying drawing 2,3), show that Fructus Foeniculi extracting solution can induce Herba Spirodelae active oxygen to produce, and cause cell membrane lipid peroxidating, and suppress Herba Spirodelae blade cell chloroplast photosynthesis effect.
The experiment effect 2 of Herba Spirodelae is controlled with Fructus Foeniculi extracting solution.
Owing to Herba Spirodelae is fluitante, the Fructus Foeniculi extracting solution that embodiment two is prepared by research is sprayed on Herba Spirodelae, studies its inhibition.In the beaker of 250ml, add 1/2 Hoagland solution of 200 ml sterilizings and Herba Spirodelae 240 lobe clean, healthy, of the same size, be placed in illumination box cultivation 6 days (temperature 25 ± 1 DEG C, light intensity 20 ~ 25 μm ol/m2/ s, Light To Dark Ratio 12/12).Use every day miniaturised nebuliser to spray 100 mg FW/ ml Fructus Foeniculi extracting solution 2 ml and 4 ml as process group, spray distilled water as a control group.Every 1-2 days statistics Herba Spirodelae lobe numbers in implementation process.Test and arrange parallel 3 groups.Result of implementation is as shown in Figure 2.Show, spray this Fructus Foeniculi extracting solution 2 ml every day and can show the obvious inhibition to Herba Spirodelae in the 7th day, and when spraying 4ml, almost can all kill Herba Spirodelae in the 7th day.Spray pattern is used can effectively to reduce the usage amount of extracting solution.
The preferred embodiment of the present invention is had been described in detail above; but, the present invention is not limited to the detail of the embodiment above, in the technology of the present invention concept; technical scheme can carry out multiple simple modification, these simple variant belong to protection scope of the present invention.
Claims (5)
1. a Fructus Foeniculi extracting solution, it is characterised in that this extracting solution is prepared as follows, specifically comprises the following steps that
A, leaf, stem and root by fresh Fructus Foeniculi air-dry after washing；
B, air-dry after Fructus Foeniculi leaf, stem and root cut into fragment, put in container；
C, Fructus Foeniculi and water are added sterilized water according to the weight ratio of 1:10 ~ 30；
Soaking at room temperature 2 ~ 4 days under d, dark condition；
E, first Fructus Foeniculi fragment is pulled out, then with 1.2 μm acetate fiber membrane filtrations, obtain Fructus Foeniculi extracting solution；
F, Fructus Foeniculi extracting solution keep in Dark Place at 4 DEG C.
A kind of Fructus Foeniculi extracting solution the most according to claim 1, it is characterised in that prepare the diameter of Fructus Foeniculi fragment in this extracting solution step b and be more preferably less than 5 mm.
A kind of Fructus Foeniculi extracting solution the most according to claim 1, it is characterised in that preparing the weight ratio of Fructus Foeniculi and water in this extracting solution step c is 1:10.
A kind of Fructus Foeniculi extracting solution the most according to claim 1, it is characterised in that preparing temperature when soaking in this extracting solution step d is 25 DEG C ± 2 DEG C, and soak time is 3 days.
5. a kind of Fructus Foeniculi extracting solution described in claim 1, it is characterised in that this Fructus Foeniculi extracting solution is for controlling the growth of Herba Spirodelae, or kills Herba Spirodelae.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610189922.0A CN105851067B (en) | 2016-03-30 | 2016-03-30 | A kind of fennel extracting solution and preparation method and the growth for being used for control duckweed |
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| CN201610189922.0A CN105851067B (en) | 2016-03-30 | 2016-03-30 | A kind of fennel extracting solution and preparation method and the growth for being used for control duckweed |
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| CN105851067B CN105851067B (en) | 2018-12-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107637442A (en) * | 2017-11-22 | 2018-01-30 | 南充有机蔬菜工程技术中心 | A kind of shared implantation methods of base of leaf of balling fennel |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107637442A (en) * | 2017-11-22 | 2018-01-30 | 南充有机蔬菜工程技术中心 | A kind of shared implantation methods of base of leaf of balling fennel |
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| CN105851067B (en) | 2018-12-18 |
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