CN105838623A - Preparation method of selenium-rich Armillaria mellea powder - Google Patents
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Abstract
The invention relates to a preparation method of selenium-rich Armillaria mellea powder, particularly a preparation method of selenium-rich Armillaria mellea powder. By using Armillaria mellea as a selenium-rich carrier, a liquid deep culture technique is adopted to perform Armillaria mellea culture metabolism within a short time to obtain the selenium-rich product. The preparation method comprises the following steps: slant Armillaria mellea strain culture: adding sodium selenite into bran boiling juice until the selenium content in the culture medium reaches 30-50 mu g/ml; shake culture: adding the bran boiling juice, sucrose, silkworm chrysalis meal, potassium dihydrogen phosphate and magnesium sulfate into a sodium selenite culture medium until the selenium content reaches 30-50 mu g/ml; fermentation culture: adding glucose, bean flour, sucrose, peptone, silkworm chrysalis meal, magnesium sulfate and potassium dihydrogen phosphate into the sodium selenite culture medium until the selenium content reaches 40 mu g/ml; and fermentation liquid separation: drying and pulverizing hyphae, concentrating the bacterium solution under reduced pressure to obtain an extract, and mixing and drying the two parts, thereby obtaining the selenium-rich Armillaria mellea powder.
Description
Technical field
The present invention relates to the preparation method of halimasch powder, more specifically, relate to the preparation method of a kind of selenium-rich halimasch powder.
Background technology
Halimasch (Armillaria mellea (vane exfr) Quel) belongs to Eumycota, Basidiomycetes, Agaricales, white mushroom
Section, Armillaria.Halimasch is the edible and medical fungi of a kind of preciousness, last century the seventies by Chinese Academy of Sciences's drug research
Institute's Pure strain separation success industrial fermentation are cultivated and are produced.It has arresting convulsion, breath wind function, to dizziness, headache, infantile convulsion,
Epilepsy, extremity numbness, soreness of waist and knee joint have the highest curative effect.Particularly it can be with gastrodia elata symbiosis and spontaneous characteristic, people
Find the existence that must there be halimasch in the growing environment of Wild gastrodia, otherwise rhizoma Gastrodiae would not grow, thus Planting now
First training rhizoma Gastrodiae is cultivated halimasch and arrow fiber crops is carried out plantation ability success the most again.Selenium is a kind of necessary trace element of human body, extensively
Be present in generally human body except fat all organs in, the shortage of selenium element in human body, it will cause angiocardiopathy, hepatopathy,
The generation of the various diseases such as diabetes, tumour respiratory system, Bones and joints, intestines and stomach, and mend selenium and can prevent even to treat these
Disease.At present to the research of human body six large effect clearly, data shows that selenium has inhibitory action to cancer cell to selenium, can reduce put,
The toxic and side effect of chemotherapy, can remove hepatitis viruse, protects liver, can strengthen the oxygen carrying capacity of hemoglobin, ensures brain, the heart
The supply of oxygen in flesh and each interior internal organs internal organs of whole body, stablizes blood fat, Sudden cardiac death that blood pressure, prevention myocardial anoxia cause.
Can form selenoprotein thrombus dissolving system in the blood vessel, reduce blood viscosity, reduce heart stalk, cerebral infarction, apoplexy, cerebral hemorrhage
Raw.Cardiac nutrition can effectively reduce diabetes patient's complication, and protection pancreas islet is injury-free.Selenium product major part is in the market
Being directly added into inorganic selenium salt in food and medicine, its biologically active is not only difficult to absorb, but also toxic, and using effect is the most not
Preferable.Finding that the area of China 2/3rds belongs to scarce selenium area according to authoritative institution's investigation, in diet structure, Se content is on the low side.Mirror
In this, it is necessary to make improvements.
Summary of the invention
In order to overcome the deficiency in the presence of prior art, it is provided that the preparation method of a kind of selenium-rich halimasch powder, the method is with honey
Ring bacterium, as selenium-rich element carrier, uses Submerged liquid culturation technology to cultivate metabolism by halimasch and obtain richness within a short period of time
Selenium product.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
The preparation method of a kind of selenium-rich halimasch powder, follows the steps below:
Inclined-plane halimasch kind is cultivated: wheat bran liquor is added sodium selenite, makes Se content in culture medium reach 30 μ g/ml-
50μg/ml；
Shaking culture: wheat bran liquor, sucrose, dried silkworm chrysalis meal, potassium dihydrogen phosphate, magnesium sulfate are joined the culture medium of sodium selenite
In, make Se content reach 30 μ g/ml-50 μ g/ml；
Fermented and cultured: glucose, bean powder, sucrose, peptone, dried silkworm chrysalis meal, magnesium sulfate, potassium dihydrogen phosphate are added selenous acid
In the culture medium of sodium, Se content is made to reach 40 μ g/ml；
Separation of fermentative broth: mycelia drying and crushing, bacterium solution reduced pressure concentration medicinal extract, then by two parts combination drying, obtain selenium-rich honey
Ring bacterium powder.
In described inclined-plane halimasch kind incubation step, 5% wheat bran liquor is joined the sodium selenite of 3-5mg/100ml, cultivate bar
Part is that 26 ± 1 DEG C of constant temperatures are cultivated 13-15 days, and inclined-plane covers with mycelia.
In described Shaking culture step, by 5% wheat bran liquor, 2% sucrose, 0.5% dried silkworm chrysalis meal, 0.15% potassium dihydrogen phosphate, 0.075% sulphur
Acid magnesium joins in the culture medium of 3-5mg/100ml sodium selenite, and Se content reaches 30 μ g/ml-50 μ g/ml, condition of culture
For 750ml shaking flask charging 200ml, rotating speed 120 revs/min, temperature 26 ± 1 DEG C is cultivated to mycelial concentration on rotary shaker
More than 90%, stop cultivating.
In described fermented and cultured step, by 1% glucose, 2% bean powder, 2% sucrose, 0.5% peptone, 0.5% dried silkworm chrysalis meal,
0.075% magnesium sulfate, 0.15% potassium dihydrogen phosphate join in the culture medium of 4mg/100ml sodium selenite, and Se content reaches
40 μ g/ml, condition of culture is 750ml shaking flask charging 200ml, and rotating speed 120 revs/min, temperature 26 ± 1 DEG C is on rotary shaker
Cultivate to mycelial concentration more than 95%, stop cultivating.
In described separation of fermentative broth step, mycelia is dried below 80 DEG C, and bacterium solution is evaporated to proportion 1.30, and two parts mix
It is dried below 80 DEG C after conjunction.
Compared with prior art the present invention is had the beneficial effect that
The present invention utilizes the characteristic of halimasch, when halimasch deep drainpipe, adds inorganic selenium salt, pass through halimasch in culture medium
Growth course obtains after Absorption And Metabolism biogenic organic selenium product.Selenium-rich halimasch powder Productive statistics cost of the present invention is few, yield
Unaffected, quality aspect increases the selenium product of useful needed by human body, brings unpredictable social effect to health of people.
Detailed description of the invention
Below by specific embodiment, the present invention is described in further detail.
Bacterial classification: select halimasch (Armillaria mellea (vane exfr) Quel).
Inclined-plane is cultivated:
5% wheat bran liquor adds the sodium selenite (NaSeO of 3～5mg/100ml2·5H2O), in culture medium, Se content reaches
30～50 μ g/ml (Se content is impacted more than 50 μ g/ml halimasch growths, poor growth).
Condition of culture: cultivate 13～15 days under 26 ± 1 DEG C of constant temperatures.Inclined-plane covers with mycelia.
When above-mentioned 5% refers to this batch of dispensing, institute's part is long-pending, and (proportion in operation is considered as
1) 5%, such as: this need to join 2000 milliliters, operates as follows: first weighs 100 grams of wheat brans and puts into graduated proportion container
In, adding water to scale, boil after stirring 30 minutes, filter by medicinal tela depurata two-layer, filtrate moisturizing obtains to scale
To the wheat bran liquid of 5%.All this is identical for following percentage, is all first to weigh solid content then to add the filtrate after boiling extremely
Scale, is sub-packed in shaking flask after stirring.
Shake flask medium:
5% wheat bran liquor, 2% sucrose, 0.5% dried silkworm chrysalis meal, 0.15% potassium dihydrogen phosphate, 0.075% magnesium sulfate add
Sodium selenite (the NaSeO of 3mg/100ml～5mg/100ml2·5H2O) in culture medium, Se content reach 30～
50μg/ml。
Condition of culture: 750ml shaking flask charging 200ml, rotating speed 120 revs/min, temperature 26 ± 1 DEG C is cultivated on rotary shaker
6～8 days.
Fermentation medium:
Glucose 1%, bean powder 2%, sucrose 2%, peptone 0.5%, dried silkworm chrysalis meal 0.5%, magnesium sulfate 0.075%, biphosphate
Potassium 0.15%, the sodium selenite (NaSeO of addition 4mg/100ml2·5H2O), in culture medium, Se content reaches
40μg/ml。
Condition of culture: 750ml shaking flask charging 200ml, 120 revs/min of cultivation 5～7 days on rotary shaker of 26 ± 1 DEG C of rotating speeds.
Separation of fermentative broth: mycelium is dried, bacterium solution is evaporated to proportion 1.30, for medicinal extract, measures medicinal extract at 60 DEG C, surveys
Determining Se content, mycelia mixes with medicinal extract, is dried, pulverizes, and is finally mixed to get selenium-rich halimasch powder.
Implementation process:
The confirmation of bacterial classification:
Bacterial classification is originated: armillaria mellea accreting with Rhizoma Gastrodiae raw material produces bacterium, and through the bacterial classification producing halimasch raw material of state approval, halimasch is ratified
Code is: traditional Chinese medicines quasi-word H14023100, and execution standard is that chemicals provincial standard rises the 13rd WS-of national standard
10001-(HD-1224)-2002。
Inclined-plane is cultivated: the 5% every 100ml of wheat bran liquor adds the Asia of 1mg, 2mg, 3mg, 4mg, 5mg, 6mg, 7mg, 8mg
Sodium selenate.Each addition does 5, observes growth and record, result:
Speed of growth measuring method: the external profile diameter of the mycelia that makes new advances to culture medium edge length centered by inclined plane inoculating point, works as inclined-plane
After laterally covering with, with longitudinal edge for measuring point.
Conclusion: through the measurement speed of growth 1,2,3,4 substantially without big difference, 5 slightly affect, 6,7,8 suppression mycelial growths.
Select 3mg～5mg/100ml relatively reasonable to the inclined-plane speed of growth.After particularly every 100ml adds 8mg, mycelia does not grows.
Shaking culture:
5% wheat bran liquor, 2% sucrose, 0.5% dried silkworm chrysalis meal, 0.15% potassium dihydrogen phosphate, 0.075% magnesium sulfate add 4
Sodium selenite (the NaSeO of mg/100ml2·5H2O) Se content in culture medium is made to reach 40 μ g/ml.750ml shaking flask feeds
200ml, rotating speed 120 revs/min, temperature 26 ± 1 DEG C is cultivated to mycelial concentration more than 90% on rotary shaker, stops cultivating.
Fermented and cultured:
Glucose 1%, bean powder 2%, sucrose 2%, peptone 0.5%, dried silkworm chrysalis meal 0.5%, magnesium sulfate 0.075%, biphosphate
Potassium 0.15%, sodium selenite (NaSeO2·5H2O)4mg/100ml.750ml shaking flask charging 200ml, rotating speed 120 revs/min,
Temperature 26 ± 1 DEG C, cultivates to mycelial concentration more than 95% on rotary shaker, and 146 hours time stopped cultivating.
One list of ingredients:
| Name of material | Proportioning | Unit | Quantity |
| Glucose | 1% | g | 100 |
| Bean powder | 2% | g | 200 |
| Sucrose | 2% | g | 200 |
| Peptone | 0.5% | g | 50 |
| Dried silkworm chrysalis meal | 0.5% | g | 50 |
| Potassium dihydrogen phosphate | 0.15% | g | 3 |
| Magnesium sulfate | 0.0175% | g | 105 |
| Sodium selenite | 4mg/100ml | mg | 80 |
| Dispensing total amount | ml | 2000 |
Operation: the most accurately weigh (during weighing, one person, a people check) with drinking water to scale, agitating heating by list of ingredients
It is sub-packed in 10 shaking flasks to 60 DEG C.120 DEG C of 30 minutes autoclavings, move to bacterial classification room after cooling.Inoculate under hundred grades of environment
15～20ml, rotary shaker cultivates rotating speed 120 revs/min, temperature 26 ± 1 DEG C, cultivates 126 hours, and mycelial concentration is the longest
More than 95%, stop cultivating.Continuous three batches, each batch 10.Separate with 90 mesh screen, mycelia drying and crushing,
Bacterium solution is concentrated into proportion 1.30 (temperature 60 C) and hypha powder combination drying, obtains selenium-rich halimasch powder, tests.
Assay:
Conclusion: perform not find differences with compareing according to halimasch production technology in whole implementation process, data analysis after testing,
Meeting halimasch powder standard-required, selenium measurement result shows that Se content, in preset range, increases Se content on the basis of original product
And function is a New function product, i.e. selenium-rich halimasch powder.
Claims (5)
1. the preparation method of a selenium-rich halimasch powder, it is characterised in that follow the steps below:
Inclined-plane halimasch kind is cultivated: wheat bran liquor is added sodium selenite, makes Se content in culture medium reach 30 μ g/ml-
50μg/ml；
Shaking culture: wheat bran liquor, sucrose, dried silkworm chrysalis meal, potassium dihydrogen phosphate, magnesium sulfate are joined the culture medium of sodium selenite
In, make Se content reach 30 μ g/ml-50 μ g/ml；
Fermented and cultured: glucose, bean powder, sucrose, peptone, dried silkworm chrysalis meal, magnesium sulfate, potassium dihydrogen phosphate are added selenous acid
In the culture medium of sodium, Se content is made to reach 40 μ g/ml；
Separation of fermentative broth: mycelia drying and crushing, bacterium solution reduced pressure concentration medicinal extract, then by two parts combination drying, obtain selenium-rich honey
Ring bacterium powder.
The preparation method of a kind of selenium-rich halimasch powder the most according to claim 1, it is characterised in that: described inclined-plane honey ring
In Spawn incubation step, 5% wheat bran liquor joining the sodium selenite of 3-5mg/100ml, condition of culture is 26 ± 1 DEG C of constant temperature
CMC model 13-15 days, inclined-plane covers with mycelia.
The preparation method of a kind of selenium-rich halimasch powder the most according to claim 1, it is characterised in that: described Shaking culture
In step, 5% wheat bran liquor, 2% sucrose, 0.5% dried silkworm chrysalis meal, 0.15% potassium dihydrogen phosphate, 0.075% magnesium sulfate are joined 3-
In the culture medium of 5mg/100ml sodium selenite, Se content reaches 30 μ g/ml-50 μ g/ml, and condition of culture is 750ml shaking flask
Charging 200ml, rotating speed 120 revs/min, temperature 26 ± 1 DEG C is cultivated on rotary shaker to mycelial concentration more than 90%, is stopped training
Support.
The preparation method of a kind of selenium-rich halimasch powder the most according to claim 1, it is characterised in that: described fermented and cultured
In step, by 1% glucose, 2% bean powder, 2% sucrose, 0.5% peptone, 0.5% dried silkworm chrysalis meal, 0.075% magnesium sulfate,
0.15% potassium dihydrogen phosphate joins in the culture medium of 4mg/100ml sodium selenite, and Se content reaches 40 μ g/ml, condition of culture
For 750ml shaking flask charging 200ml, rotating speed 120 revs/min, temperature 26 ± 1 DEG C is cultivated to mycelial concentration on rotary shaker
More than 95%, stop cultivating.
The preparation method of a kind of selenium-rich halimasch powder the most according to claim 1, it is characterised in that: described zymotic fluid divides
In step, mycelia is dried below 80 DEG C, and bacterium solution is evaporated to proportion 1.30, dry below 80 DEG C after two parts mixing
Dry.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723322A (en) * | 2017-11-28 | 2018-02-23 | 陕西省微生物研究所 | A kind of preparation method of amillariella mellea selenium germanium polysaccharide |
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| CN102579509A (en) * | 2012-01-18 | 2012-07-18 | 山西康欣药业有限公司 | Selenium-enriched marasmius androsaceus toadstool extract preparation and preparation method thereof |
| CN103250568A (en) * | 2013-05-30 | 2013-08-21 | 安徽鑫农食用菌有限公司 | Method for preparing hericium erinaceus rich in selenium |
| CN105146058A (en) * | 2015-09-17 | 2015-12-16 | 天津中天精科科技有限公司 | Feed additive product |
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2016
- 2016-04-15 CN CN201610237063.8A patent/CN105838623A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102579509A (en) * | 2012-01-18 | 2012-07-18 | 山西康欣药业有限公司 | Selenium-enriched marasmius androsaceus toadstool extract preparation and preparation method thereof |
| CN103250568A (en) * | 2013-05-30 | 2013-08-21 | 安徽鑫农食用菌有限公司 | Method for preparing hericium erinaceus rich in selenium |
| CN105146058A (en) * | 2015-09-17 | 2015-12-16 | 天津中天精科科技有限公司 | Feed additive product |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107723322A (en) * | 2017-11-28 | 2018-02-23 | 陕西省微生物研究所 | A kind of preparation method of amillariella mellea selenium germanium polysaccharide |
| CN107723322B (en) * | 2017-11-28 | 2021-03-02 | 陕西省微生物研究所 | Preparation method of armillaria selenogermani polysaccharide |
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