CN105837849A - Preparation method of functionalized composite porous material - Google Patents
Preparation method of functionalized composite porous material Download PDFInfo
- Publication number
- CN105837849A CN105837849A CN201610300686.5A CN201610300686A CN105837849A CN 105837849 A CN105837849 A CN 105837849A CN 201610300686 A CN201610300686 A CN 201610300686A CN 105837849 A CN105837849 A CN 105837849A
- Authority
- CN
- China
- Prior art keywords
- preparation
- porous material
- pore creating
- plga
- composite porous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/26—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/0066—Use of inorganic compounding ingredients
- C08J9/0071—Nanosized fillers, i.e. having at least one dimension below 100 nanometers
- C08J9/0076—Nanofibres
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K7/00—Use of ingredients characterised by shape
- C08K7/22—Expanded, porous or hollow particles
- C08K7/24—Expanded, porous or hollow particles inorganic
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/011—Nanostructured additives
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Nanotechnology (AREA)
- Inorganic Chemistry (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
Abstract
The invention provides a preparation method of a functionalized composite porous material. The method comprises the following steps that 1, pore-forming agent suspension liquid is flatwise laid on the carrier surface, and after drying is conducted, a pore-forming agent template attached to the carrier surface is obtained; 2, a matrix solution is laid on the pore-forming agent template, and drying is conducted to obtain a composite casting film, wherein the matrix solution is prepared from a polylactic acid-glycolic acid copolymer, carbon nanotubes and a solvent; 3, the composite casting film is immersed into an acid solution, and the functionalized composite porous material is obtained. According to the preparation method, the porous scaffold material is prepared by combining a particle dissolution method with a casting film method, and the prepared porous material can simulate the extracellular matrix in the form and function and has the good biocompatibility and the good mechanical strength; experiment results show that the tensile break stress of the functionalized composite porous material prepared through the preparation method is 3.5-4.1 MPa, and the elongation at break of the functionalized composite porous material prepared through the preparation method is about 75.16%.
Description
Technical field
The invention belongs to porous material technical field, particularly relate to the system of a kind of functionalization composite porous material
Preparation Method.
Background technology
Biomedical applications mainly includes tissue engineering bracket, drug release, biosensor, medical plants
Enter thing, affinity membrane and wound dressing etc..In recent years, human health side is being promoted due to organizational project
Face shows unlimited potential, and therefore, the research to them also achieves significant progress.To organize work
As a example by journey, tissue, the defect of organ or the dysfunction that disease and wound cause is that human health is faced
One of main harm.How to overcome present in autologous or allosome tissue, organ transplantation " with repair in trauma
Wound ", the defect such as donor source is not enough, fundamentally solve tissue, organ defect reparation and reconstruction
Etc. problem, it has also become the international advanced subject of life science.Wherein there is the porous material of functionalization
The preparation of material is one of key technology of histoorgan recovery project.
During as tissue engineering bracket, the porous material of functionalization can for the sticking of cell, grow, move
Move and breed the template providing good.Therefore, by means of the development of modern science and technology, how to make impaired
Histoorgan obtain regeneration completely or copy required tissue in vitro or organ carries out substituting controlling
Treatment becomes as biology, preclinical medicine and clinical medicine focus of attention, not only has important scientific value,
And the hugest application prospect.
Porous polymer film material is widely used in tissue engineering bracket, because these materials can be with mould
Intend the physical characteristic of natural extracellular matrix (ECM).In organizational project, it is good that polymer support has connection
Good loose structure, they provide interim physical template for cell, it is simple to cell adhesion, migrate, increase
Grow, break up, neoblastic regeneration and three dimensional structure.Finally, support should be by the natural fine of emiocytosis
Extracellular matrix is substituted.Biodegradable high polymer is a kind of candidate material having very much allure, because it
Have structured integrity, last natural decomposition, leave the tissue being newly formed, tissue will be undertaken required
The mechanical load wanted.Porous material is as stenter to implant animal or human body, it is necessary to possess two functions, its
One is to have certain mechanical strength to keep former organized shape；It two is to have certain biological live
Property induced cell growth and differentiation, thus guide the regeneration of damaged tissues, and timbering material finally can quilt
Degraded and absorbed.
At present, preparing porous support materials has a lot of method, mainly have particle leaching, fibres bond,
Gas foaming, lyophilization, 3 D-printing and be separated etc. preparation method.Though these preparation methoies respectively have
Feature but still have weak point.Such as, particle leaching is to add soluble granule conduct in the base
Pore creating material, such as Sal, sucrose, mannitol, sodium bicarbonate, sodium dihydrogen phosphate and sodium glutamate etc., treats
Matrix solidification after be soaked in particular solution so that it is in pore creating material dissolution and leave hole.This method
Simple, but, the porous support materials mechanical strength that the method prepares is the highest.
Summary of the invention
The invention provides the preparation method of a kind of functionalization composite porous material, the preparation side in the present invention
The functionalization composite porous material that method obtains has higher mechanical strength.
The present invention provides the preparation method of a kind of functionalization composite porous material, comprises the following steps:
A) pore creating material suspension is laid in carrier surface, obtains being attached to the pore-creating of carrier surface after drying
Agent template；
B) Matrix Solution is taped against in described pore creating material template, obtains compound casting films after drying；
Described Matrix Solution includes matrix material, CNT and solvent；
Described matrix material includes in PLGA, polylactic acid, fibroin albumen and chitosan
One or more；
C) described compound casting films is immersed in acid solution, obtain functionalization composite porous material.
Preferably, described pore creating material suspension is selected from melamine resin microsphere suspension liquid.
Preferably, in described pore creating material suspension, the particle diameter of pore creating material is 100～10000nm.
Preferably, the mass concentration of described pore creating material suspension is 3～5%.
Preferably, in described Matrix Solution, the mass concentration of PLGA is (0.06～0.14)
g/mL。
Preferably, described CNT is (0.1～1) with the mass ratio of PLGA: 100.
Preferably, described solvent is oxolane and the mixed solution of dimethylformamide, hexafluoroisopropanol
Or chloroform；
Described oxolane and oxolane in the mixed solution of dimethylformamide and dimethylformamide
Volume ratio is (1～5): 1.
Preferably, described PLGA with the volume ratio of pore creating material in pore creating material suspension is
(2～3): 1.
Preferably, described step B) particularly as follows:
Matrix Solution is taped against in pore creating material template, obtains hygrometric state casting films, then described hygrometric state is cast
Film vibrates, and obtains compound casting films after drying.
Preferably, the time of described vibration is 10～60min.
The invention provides the preparation method of a kind of functionalization composite porous material, comprise the following steps: A)
Pore creating material suspension is laid in carrier surface, obtains being attached to the pore creating material template of carrier surface after drying；
B) Matrix Solution is taped against in described pore creating material template, obtains compound casting films after drying；Described matrix is molten
Liquid includes PLGA, CNT and solvent.C) described compound casting films is immersed acid
In property solution, obtain functionalization composite porous material.Particle leaching is tied mutually by the present invention with curtain coating embrane method
Porous material is prepared in conjunction, prepared porous material form and functionally can analog cell epimatrix, and
And have good biocompatibility and good mechanical strength, test result indicate that, the preparation in the present invention
The functionalization composite porous material tensile break stress that method prepares is 3.5～4.1MPa, and elongation at break exists
About 75.16%.It addition, the preparation method preparation process in the present invention is simple, pore size is easily controlled
There is the structural stability of good pattern.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to reality
Execute the required accompanying drawing used in example or description of the prior art to be briefly described, it should be apparent that below,
Accompanying drawing in description is only embodiments of the invention, for those of ordinary skill in the art, not
On the premise of paying creative work, it is also possible to obtain other accompanying drawing according to the accompanying drawing provided.
Fig. 1 is the scanning of the PLGA/MWCNTs functionalization composite porous material that the embodiment of the present invention 1 obtains
Electronic Speculum figure；
Fig. 2 is the scanning of the PLGA/MWCNTs functionalization composite porous material that the embodiment of the present invention 2 obtains
Electronic Speculum figure；
Fig. 3 is that cell is compound porous in the PLGA/MWCNTs functionalization of the embodiment of the present invention 1, embodiment 2
Adhesive tendencies on material and blank slide；
Fig. 4 is that cell is compound porous in the PLGA/MWCNTs functionalization of the embodiment of the present invention 1, embodiment 2
Proliferative conditions on material and blank slide；
Fig. 5 is on cell PLGA/MWCNTs functionalization composite porous material in the embodiment of the present invention 2
Adhere to and the scanning electron microscope (SEM) photograph of propagation；
Fig. 6 is the scanning of the PLGA/SWCNTs functionalization composite porous material that the embodiment of the present invention 3 obtains
Electronic Speculum figure.
Detailed description of the invention
The invention provides the preparation method of a kind of functionalization composite porous material, comprise the following steps:
A) pore creating material suspension is laid in carrier surface, obtains being attached to the pore-creating of carrier surface after drying
Agent template；
B) Matrix Solution is taped against in described pore creating material template, obtains compound casting films after drying；
Described Matrix Solution includes PLGA, CNT and solvent.
C) described compound casting films is immersed in acid solution, obtain functionalization composite porous material.
The functionalization composite porous material that preparation method in the present invention obtains has higher mechanical strength.
Pore creating material suspension is laid in carrier surface by the present invention, obtains after drying being attached to carrier surface
Pore creating material template, in the present invention, described pore creating material suspension is the unit for uniform suspension of pore creating material and water,
Described pore creating material suspension preferably includes melamine resin microsphere suspension liquid；Described pore creating material suspends
The mass concentration of liquid is preferably 3～5%, more preferably 3.5～4.5%, and most preferably 4%；Described pore creating material
Particle diameter be preferably 100～10000nm, more preferably 1000～9000nm, concrete, the present invention's
In embodiment, can be 2 μm or 7 μm, the particle diameter provided such as Berlin, Germany microgranule company limited be respectively
It is 2 ± 0.06 μm, the melamine resin microsphere of 7 ± 0.15 μm.
The present invention does not has special restriction to the kind of described carrier, it is preferred to use smooth the consolidating of surfacing
Body carrier, such as glass slide.In the present invention, described dry temperature preferably 20～40 DEG C, more preferably
25～35 DEG C；The described dry time is preferably 24～48 hours, more preferably 30～40 hours；Obtain
Pore creating material template is substantially attached to pore creating material microsphere layer on glass slide, and described pore creating material template can be
Monolayer, it is also possible to be multilamellar, depend on the needs.
After obtaining pore creating material template, Matrix Solution is taped against described pore creating material template surface by the present invention, is dried
After obtain compound casting films, Matrix Solution is preferably taped against in pore creating material template by the present invention, obtains hygrometric state stream
Prolong film, then described hygrometric state casting films is vibrated, obtain compound casting films after drying.To described hygrometric state stream
Prolonging film and carrying out the purpose of slight oscillatory is the gap allowing Matrix Solution to be completely filled with described pore creating material template.
The time of described vibration is preferably 10～60min, more preferably 20～50min, most preferably 30～40min；
The frequency of described vibration is preferably 120～240 times/min, more preferably 150～200 times/min.
In the present invention, described Matrix Solution includes matrix material, CNT and solvent, described matrix
Material includes that PLGA (PLGA), polylactic acid (PLA), fibroin albumen and shell are poly-
One or more in sugar；Wherein, the molecular weight of PLGA is 81000g/mol；Viscosity is 0.61dL/g.
Described CNT can be SWCN (SWCNTs), it is also possible to be multi-walled carbon nano-tubes
(MWCNTs)；The diameter of described CNT is preferably 30～70nm, more preferably 40～60nm,
Most preferably 45～55nm；The length of described CNT is preferably 100～400nm, more preferably
150～350nm, most preferably 200～300nm.The matter of PLGA in described Matrix Solution
Amount concentration is preferably (0.06～0.14) g/mL, and more preferably (0.08～0.12) g/mL, concrete,
In embodiments of the invention, can be 0.06g/mL, 0.08g/mL, 0.10g/mL, 0.12g/mL or
0.14g/mL.Described CNT is preferably (0.1～1) with the mass ratio of PLGA:
100, more preferably (0.2～0.9): 100, concrete, in an embodiment of the present invention, can be 0.5:100.
Described solvent is preferably the mixed solution of oxolane and dimethylformamide, described oxolane and diformazan
The volume ratio of base Methanamide is preferably (1～5): 1, more preferably (2～4): 1, most preferably 3:1.
In the present invention, in described pore-creating and suspension, the volume ratio of pore creating material and described matrix material is preferred
For 1:(2～3), more preferably 1:2.5.
The present invention preferably by PLGA when stirring, add oxolane and two
In the mixed organic solvents of methylformamide, fully dissolve, form PLGA solution, the most again carbon is received
Mitron, under conditions of stirring, is dispersed in PLGA solution, obtains Matrix Solution, i.e.
PLGA/CNTs solution.
In the present invention, described being dried is preferably vacuum drying, and described vacuum drying vacuum is preferably
0.02MPa；The described vacuum drying time is preferably 24～48 hours；Described vacuum drying temperature is preferred
It it is 20～40 DEG C.
After obtaining compound casting films, described compound casting films is preferably immersed in acid solution by the present invention,
To functionalization composite porous material.Described acid solution preferably includes hydrochloric acid solution or sulfuric acid solution；Described
The molar concentration of acid solution is preferably 0.5～2mol/L, more preferably 1mol/L, by described compound curtain coating
After film immerses acid solution, after 0.5～2 hour, pore creating material dissolves, and carrier and compound casting films completely disengage from,
Again the compound casting films of described disengaging is carried out and is vacuum dried, obtain functionalization composite porous material.
In the present invention, the concentration of acid solution and the dissolution time of pore creating material need to strictly be controlled, during dissolving
Between long, easily cause porous material to deform, dissolution time is too short, make pore creating material dissolve insufficient, this
Pore creating material dissolution time in invention is preferably 0.5～2 hour, more preferably 1～1.5 hour；Acid solution
Concentration is excessive, can destroy porous material, and concentration is too small, can affect the dissolving of pore creating material.
According to above-mentioned preparation method prepare for circular, membranaceous functionalization composite porous material, its thickness
It is preferably 0.001～0.1cm, more preferably 0.002～0.05cm.
Preparation method in the present invention has the advantage that
(1) curtain coating embrane method and particle leaching is utilized to prepare PLGA/CNTs functionalization composite porous material,
Its mechanical performance is made to be significantly improved；Additionally material central hole structure be formed by microsphere dissolution and reality
Existing, the most particularly suitable prepare the porous material that thickness is less；
(2) by using the microsphere of different-grain diameter, it is achieved the controlled preparation to aperture of porous material, hole shape
Looks and structural stability are good, and porosity is 67%～about 70.1%；
(3) micro nano structure of PLGA/CNTs functionalization composite porous material has bigger specific surface
Long-pending (0.2～0.5m2/ g), the three-dimensional porous structure of its uniqueness can promote adhesion and the propagation of cell.
In order to further illustrate the present invention, a kind of functionalization provided the present invention below in conjunction with embodiment is multiple
Conjunction porous material is described in detail, but can not be understood as limiting the scope of the present invention.
In the examples below, PLGA is purchased from Jinan Dai Gang biologic material products company, melamino-formaldehyde
Resin microsphere (MF) suspension provides for Berlin, Germany microgranule company limited, and microspherulite diameter is respectively
2 ± 0.06 μm, 7 ± 0.15 μm.
Porosity computing formula:
In formula, m, r and h represent the quality of perforated membrane, radius and thickness respectively.ρbBasis for polymer
Body density, ε is porosity.
Embodiment 1
By the PLGA (PLGA) of 1g under stirring, join oxolane with
Dimethylformamide volume ratio is in the mixed organic solvents of 3:1, fully dissolves, and is made into concentration and is
The solution of 0.1g/mL；The MWCNTs of 0.005g is dispersed in PLGA solution, makes
PLGA/MWCNTs mixed solution.Measure the melamine resin microsphere that 5 μ L particle diameters are 2 μm
(MF) suspension, is laid on the circular slide fully cleaned, and is completely dispersed and is dried, is attached to
MF microsphere template on slide.The PLGA/MWCNTs mixed solution measuring 10 μ L again is taped against MF
On microsphere template, then by the hygrometric state casting films slight oscillatory 30 minutes of mixed solution/MF template, purpose
It is the gap allowing mixed solution to be completely filled with MF colloid template, vacuum drying 48 hours subsequently；Finally,
The slide of the compound PLGA/MWCNTs/MF casting films being dried immerses in the hydrochloric acid solution of 1mol/L,
After 1 hour, MF microsphere dissolves, and slide and PLGA/MWCNTs casting films completely disengage from, the most clearly
Wash casting films, be then vacuum dried, obtain the membranaceous PLGA/MWCNTs of circle that thickness is 0.003cm
Functionalization composite porous material.
Porous material quality m that the present embodiment obtains is about 0.001g, radius r and is about 0.5cm, thickness h
It is about 0.003cm, ρbFor 1.25g/cm3.Calculate according to above-mentioned formula, the porous material in the present embodiment
Porosity is 67%.
PLGA/MWCNTs functionalization composite porous material pattern in the present embodiment is as it is shown in figure 1, scheme
The scanning electron microscope of the PLGA/MWCNTs functionalization composite porous material that 1 obtains for the embodiment of the present invention 1
Figure.As seen from Figure 1, the hole of PLGA/MWCNTs functionalization composite porous material in the present embodiment
Footpath is 2 μm.
Measuring (Bei Shide instrument) through specific surface instrument, in the present embodiment, PLGA/MWCNTs functionalization is multiple
The specific surface area closing porous material is 0.2～0.5m2/g。
The present invention uses universal testing machine (INSTRON 3365) to test in the present embodiment
The mechanical performance of PLGA/MWCNTs functionalization composite porous material, its tensile break stress is
3.5MPa, elongation at break is 60.5%.
Embodiment 2
By the PLGA (PLGA) of 1.2g under stirring, join oxolane with
Dimethylformamide volume ratio is in the mixed organic solvents of 3:1, fully dissolves, and is made into concentration and is
The solution of 0.12g/mL；The MWCNTs of 0.012g is dispersed in PLGA solution, makes
PLGA/MWCNTs mixed solution；Measure the melamine resin microsphere that 5 μ L particle diameters are 7 μm
(MF) suspension, is laid on the circular slide fully cleaned, and is completely dispersed and is dried, is attached to
MF microsphere template on slide.The PLGA/MWCNTs mixed solution measuring 10 μ L again is taped against MF
On microsphere template, then by the hygrometric state casting films slight oscillatory 20 minutes of mixed solution/MF template, purpose
It is the gap allowing mixed solution to be completely filled with MF colloid template, vacuum drying 48 hours subsequently；Finally,
The slide of the compound PLGA/MWCNTs/MF casting films being dried immerses in the hydrochloric acid solution of 1mol/L,
After 1.5 hours, MF microsphere dissolves, and slide and PLGA/MWCNTs casting films completely disengage from, the most clearly
Wash casting films, be then vacuum dried, obtain the membranaceous PLGA/MWCNTs of circle that thickness is 0.0039cm
Functionalization composite porous material.
Porous material quality m that the present embodiment obtains is about 0.0012g, radius r and is about 0.5cm, thickness
H is about 0.0039cm, ρbFor 1.25g/cm3.Calculate according to above-mentioned formula, the porous material in the present embodiment
Material porosity is 69%.
PLGA/MWCNTs functionalization composite porous material pattern in the present embodiment is as in figure 2 it is shown, scheme
The scanning electron microscope of the PLGA/MWCNTs functionalization composite porous material that 2 obtain for the embodiment of the present invention 2
Figure.As shown in Figure 2, in the present embodiment, the aperture of PLGA/MWCNTs functionalization composite porous material is
7μm。
Measuring (Bei Shide instrument) through specific surface instrument, in the present embodiment, PLGA/MWCNTs functionalization is multiple
The specific surface area closing porous material is 0.05～0.1m2/g。
The present invention uses universal testing machine (INSTRON 3365) to test in the present embodiment
The mechanical performance of PLGA/MWCNTs functionalization composite porous material, its tensile break stress is
4.1MPa, elongation at break is 75.16%.
The compound porous material of PLGA/MWCNTs functionalization that Example 1 and Example 2 of the present invention is obtained
24 well culture plates put into by material and blank slide, add 75% ethanol, soaking disinfection 4 hours, then
Sucking-off ethanol, dries, with PBS solution soak rinsing 3 times, each 20 minutes.Then DMEM is used
About culture medium soak-out material half an hour, then suck culture medium, put into CO2After incubator a few hours,
Inoculate according to the cell density in 20000, every hole.The cell cultivating certain time is taken out from incubator,
Liquid-transfering gun sops up original culture fluid, rinses 3 times by the PBS solution through preheating, and every hole adds preheating
To 37 DEG C not added with 10% hyclone and dual anti-DMEM culture medium 360 μ L, add and be preheating to 37 DEG C
MTT 40 μ L, cultivate 4 hours, will have purple precipitation produce, then culture medium is sucked, every hole adds
Enter 400 μ L DMSO, dissolution precipitation 20 minutes, become purple solution, then pipette solution with liquid-transfering gun
100 μ L add in every hole of 96 well culture plates, detect wavelength 570nm in Multifunction fluorescent microplate reader
The fluorescent value at place, what size can reflect living cells sticks trend.
As seen in figures 3-5, Fig. 3 is that cell is at the embodiment of the present invention 1, embodiment 2 and blank slide to result
On adhesive tendencies, wherein, the adhesive tendencies that 1 is cell on blank slide, 2 is that cell is in the present invention
The adhesive tendencies on PLGA/MWCNTs functionalization composite porous material in embodiment 1,3 is cell
The adhesive tendencies on PLGA/MWCNTs functionalization composite porous material in the embodiment of the present invention 2.
As seen from Figure 3, prolongation over time, compared with the blank slide, cell is on the porous material
Stick more and more；Fig. 4 is that cell is at the embodiment of the present invention 1, the PLGA/MWCNTs of embodiment 2
Proliferative conditions on functionalization composite porous material and blank slide, wherein, 1 is that cell is on blank slide
Proliferative conditions, 2 is that the PLGA/MWCNTs functionalization in the cell embodiment of the present invention 1 is compound porous
Proliferative conditions on material, 3 is that the PLGA/MWCNTs functionalization in the cell embodiment of the present invention 2 is multiple
Close the proliferative conditions on porous material.As seen from Figure 4, along with the increase of cultivated days, with blank
Slide is compared, and cell proliferative conditions on the porous material is good.Fig. 5 is that cell is in the embodiment of the present invention 2
In PLGA/MWCNTs functionalization composite porous material on the scanning electron microscope (SEM) photograph that adheres to and breed.
Embodiment 3
By the PLGA (PLGA) of 1g under stirring, join oxolane and two
Methylformamide volume ratio is in the mixed organic solvents of 3:1, fully dissolves, and being made into concentration is 0.1g/mL
Solution；The SWCNTs of 0.01g is dispersed in PLGA solution, makes PLGA/SWCNTs
Mixed solution；Measure melamine resin microsphere (MF) suspension that 5 μ L particle diameters are 7 μm, flat
Being laid on the circular slide fully cleaned, be completely dispersed and be dried, the MF obtaining being attached on slide is micro-
Ball template.The PLGA/SWCNTs mixed solution measuring 10 μ L again is taped against on MF microsphere template, so
After by the hygrometric state casting films slight oscillatory 20 minutes of mixed solution/MF template, it is therefore an objective to allow mixed solution
It is completely filled with the gap of MF colloid template, subsequently vacuum drying 48 hours；Finally, be dried is compound
The slide of PLGA/SWCNTs/MF casting films immerses in the hydrochloric acid solution of 1mol/L, after 1.5 hours,
MF microsphere dissolves, and slide and PLGA/MWCNTs casting films completely disengage from, and fully clean casting films,
Then it is vacuum dried, obtains the membranaceous PLGA/MWCNTs functionalization of circle that thickness is 0.0034cm multiple
Close porous material.
Porous material quality m in the present embodiment is about 0.001g, radius r and is about 0.5cm, thickness h
It is about 0.0034cm, ρbFor 1.25g/cm3.Being computed, the porosity of porous material in the present embodiment is
70.1%.
PLGA/SWCNTs functionalization composite porous material pattern in the present embodiment as shown in Figure 6, is schemed
The scanning electron microscope of the PLGA/SWCNTs functionalization composite porous material that 6 obtain for the embodiment of the present invention 3
Figure.It will be appreciated from fig. 6 that the aperture of PLGA/SWCNTs functionalization composite porous material is in the present embodiment
7μm。
Measuring (Bei Shide instrument) through specific surface instrument, in the present embodiment, PLGA/SWCNTs functionalization is multiple
The specific surface area closing porous material is 0.1～0.3m2/g。
The present invention uses universal testing machine (INSTRON 3365) to test in the present embodiment
The mechanical performance of PLGA/SWCNTs functionalization composite porous material, its tensile break stress is 3.8MPa,
Elongation at break is 69.3%.
The above is only the preferred embodiment of the present invention, it is noted that general for the art
For logical technical staff, under the premise without departing from the principles of the invention, it is also possible to make some improvement and profit
Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a preparation method for functionalization composite porous material, comprises the following steps:
A) pore creating material suspension is laid in carrier surface, obtains being attached to the pore-creating of carrier surface after drying
Agent template；
B) Matrix Solution is taped against in described pore creating material template, obtains compound casting films after drying；
Described Matrix Solution includes matrix material, CNT and solvent；
Described matrix material includes in PLGA, polylactic acid, fibroin albumen and chitosan
One or more；
C) described compound casting films is immersed in acid solution, obtain functionalization composite porous material.
Preparation method the most according to claim 1, it is characterised in that described pore creating material suspension selects
From melamine resin microsphere suspension liquid.
Preparation method the most according to claim 2, it is characterised in that in described pore creating material suspension
The particle diameter of pore creating material is 100～10000nm.
Preparation method the most according to claim 1, it is characterised in that described pore creating material suspension
Mass concentration is 3～5%.
Preparation method the most according to claim 1, it is characterised in that poly-breast in described Matrix Solution
The mass concentration of acid ethanol copolymer is (0.06～0.14) g/mL.
Preparation method the most according to claim 4, it is characterised in that described CNT and poly-breast
The mass ratio of acid ethanol copolymer is (0.1～1): 100.
Preparation method the most according to claim 1, it is characterised in that described solvent is oxolane
Mixed solution, hexafluoroisopropanol or chloroform with dimethylformamide；
Described oxolane and oxolane in the mixed solution of dimethylformamide and dimethylformamide
Volume ratio is (1～5): 1.
Preparation method the most according to claim 1, it is characterised in that described poly (lactic acid-glycolic acid) is altogether
Polymers is (2～3) with the volume ratio of pore creating material in pore creating material suspension: 1.
9. according to the preparation method described in claim 1～8 any one, it is characterised in that described step
B) particularly as follows:
Matrix Solution is taped against in pore creating material template, obtains hygrometric state casting films, then described hygrometric state is cast
Film vibrates, and obtains compound casting films after drying.
Preparation method the most according to claim 9, it is characterised in that the time of described vibration is
10～60min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610300686.5A CN105837849B (en) | 2016-05-09 | 2016-05-09 | A kind of preparation method of functionalization composite porous material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610300686.5A CN105837849B (en) | 2016-05-09 | 2016-05-09 | A kind of preparation method of functionalization composite porous material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105837849A true CN105837849A (en) | 2016-08-10 |
| CN105837849B CN105837849B (en) | 2019-04-26 |
Family
ID=56591162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610300686.5A Active CN105837849B (en) | 2016-05-09 | 2016-05-09 | A kind of preparation method of functionalization composite porous material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105837849B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987686A (en) * | 2017-12-22 | 2018-05-04 | 枞阳县三金颜料有限责任公司 | A kind of water paint of high film extensibility |
| CN115715824A (en) * | 2022-10-12 | 2023-02-28 | 杭州洛兮生物科技有限公司 | Bone scaffold material based on modified single-walled carbon nanotubes and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004071949A2 (en) * | 2003-02-13 | 2004-08-26 | The Regents Of The University Of California | Nanostructured casting of organic and bio-polymers in porous silicon templates |
| US20090114089A1 (en) * | 2007-11-02 | 2009-05-07 | Chunqing Liu | Microporous Aluminophosphate Molecular Sieve Membranes for Highly Selective Separations |
| CN101979103A (en) * | 2010-10-26 | 2011-02-23 | 中南大学 | Method for preparing porous tissue engineering scaffold |
| CN103638561A (en) * | 2013-11-29 | 2014-03-19 | 苏州大学 | Preparation method of micro-nano bioactive porous material |
-
2016
- 2016-05-09 CN CN201610300686.5A patent/CN105837849B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004071949A2 (en) * | 2003-02-13 | 2004-08-26 | The Regents Of The University Of California | Nanostructured casting of organic and bio-polymers in porous silicon templates |
| US20090114089A1 (en) * | 2007-11-02 | 2009-05-07 | Chunqing Liu | Microporous Aluminophosphate Molecular Sieve Membranes for Highly Selective Separations |
| CN101979103A (en) * | 2010-10-26 | 2011-02-23 | 中南大学 | Method for preparing porous tissue engineering scaffold |
| CN103638561A (en) * | 2013-11-29 | 2014-03-19 | 苏州大学 | Preparation method of micro-nano bioactive porous material |
Non-Patent Citations (2)
| Title |
|---|
| 潘志娟: "《纤维材料近代测试技术》", 30 September 2005 * |
| 许海燕等: "《纳米生物医学技术》", 28 February 2009 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987686A (en) * | 2017-12-22 | 2018-05-04 | 枞阳县三金颜料有限责任公司 | A kind of water paint of high film extensibility |
| CN115715824A (en) * | 2022-10-12 | 2023-02-28 | 杭州洛兮生物科技有限公司 | Bone scaffold material based on modified single-walled carbon nanotubes and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105837849B (en) | 2019-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Geetha Bai et al. | Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering | |
| Huang et al. | Triple-layer vascular grafts fabricated by combined E-Jet 3D printing and electrospinning | |
| Taskin et al. | Three-dimensional polydopamine functionalized coiled microfibrous scaffolds enhance human mesenchymal stem cells colonization and mild myofibroblastic differentiation | |
| Park et al. | Creation of a hybrid scaffold with dual configuration of aligned and random electrospun fibers | |
| Wang et al. | Coaxial extrusion of tubular tissue constructs using a gelatin/GelMA blend bioink | |
| Sharifi et al. | Polycaprolactone microfibrous scaffolds to navigate neural stem cells | |
| Abel et al. | Combination of electrospinning with other techniques for the fabrication of 3D polymeric and composite nanofibrous scaffolds with improved cellular interactions | |
| Uttayarat et al. | Micropatterning of three-dimensional electrospun polyurethane vascular grafts | |
| Kim et al. | Hybrid process for fabricating 3D hierarchical scaffolds combining rapid prototyping and electrospinning | |
| US8815276B2 (en) | Three-dimensional nanostructured hybrid scaffold and manufacture thereof | |
| Safaeijavan et al. | Biological behavior study of gelatin coated PCL nanofiberous electrospun scaffolds using fibroblasts | |
| Yao et al. | Strategies to improve nanofibrous scaffolds for vascular tissue engineering | |
| Burton et al. | The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells | |
| Beckett et al. | Enhancement of the mechanical properties of hydrogels with continuous fibrous reinforcement | |
| Pooyan et al. | Design of a cellulose-based nanocomposite as a potential polymeric scaffold in tissue engineering | |
| Jing et al. | Electrospinning homogeneous nanofibrous poly (propylene carbonate)/gelatin composite scaffolds for tissue engineering | |
| Lee et al. | Sliding fibers: slidable, injectable, and gel-like electrospun nanofibers as versatile cell carriers | |
| Rajasekaran et al. | Role of nanofibers on MSCs fate: Influence of fiber morphologies, compositions and external stimuli | |
| Babu et al. | Controlling structure with injectable biomaterials to better mimic tissue heterogeneity and anisotropy | |
| Tian et al. | Fabrication of nanocomposite bioelastomer porous scaffold based on chitin nanocrystal supported emulsion-freeze-casting | |
| Salifu et al. | Electrospinning of nanocomposite fibrillar tubular and flat scaffolds with controlled fiber orientation | |
| Malladi et al. | Continuous formation of ultrathin, strong collagen sheets with tunable anisotropy and compaction | |
| Wood et al. | Wet-laid soy fiber reinforced hydrogel scaffold: Fabrication, mechano-morphological and cell studies | |
| Kalhori et al. | Cardiovascular 3D bioprinting: A review on cardiac tissue development | |
| CN105837849A (en) | Preparation method of functionalized composite porous material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |