CN105837849B - A kind of preparation method of functionalization composite porous material - Google Patents

A kind of preparation method of functionalization composite porous material Download PDF

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CN105837849B
CN105837849B CN201610300686.5A CN201610300686A CN105837849B CN 105837849 B CN105837849 B CN 105837849B CN 201610300686 A CN201610300686 A CN 201610300686A CN 105837849 B CN105837849 B CN 105837849B
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pore creating
porous material
creating material
preparation method
composite porous
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CN105837849A (en
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刘福娟
王萍
徐岚
何吉欢
张岩
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苏州大学
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Abstract

The present invention provides a kind of preparation methods of functionalization composite porous material, comprising the following steps: A) pore creating material suspension is laid in carrier surface, the pore creating material template for being attached to carrier surface is obtained after dry;B) Matrix Solution is taped against in the pore creating material template, obtains compound casting films after dry;Described matrix solution includes polylactide glycolate copolymer, carbon nanotube and solvent.C) the compound casting films are immersed in acid solution, obtain functionalization composite porous material.Particle leaching is combined with curtain coating embrane method and prepares porous support materials by the present invention, porous material obtained can simulate extracellular matrix in form and function, and there are good biocompatibility and good mechanical strength, the experimental results showed that, functionalization composite porous material tensile break stress made from preparation method in the present invention is 3.5~4.1MPa, and elongation at break is 75.16% or so.

Description

A kind of preparation method of functionalization composite porous material

Technical field

The invention belongs to porous material technical field more particularly to a kind of preparation methods of functionalization composite porous material.

Background technique

Biomedical applications mainly include tissue engineering bracket, drug release, biosensor, medical implant, affine Film and wound dressing etc..In recent years, unlimited potential is shown in terms of promoting human health due to organizational project, Significant progress is also achieved to their research.By taking organizational project as an example, the defect of tissue, organ caused by disease and wound Or dysfunction is one of the main harm that human health is faced.How to overcome in self or allosome tissue, organ transplant and deposits " with wound repair wound ", donor source is insufficient the defects of, fundamentally solve tissue, organ defect reparation and function weight The problems such as building, it has also become the international advanced subject of life science.Wherein having the preparation of the porous material of functionalization is One of the key technology of histoorgan recovery project.

When as tissue engineering bracket, the porous material of functionalization can for cell stick, grow, migrating and proliferation mentions For good template.Therefore, by means of the development of modern science and technology, how to make impaired histoorgan obtain regeneration completely or Tissue or organ required for copying in vitro carry out surrogate therapeutic and become as biology, preclinical medicine and clinical medicine pass The focus of note not only has important scientific value, but also there are also huge application prospects.

Porous polymer film material is widely used in tissue engineering bracket, because these materials can simulate natural fine The physical characteristic of extracellular matrix (ECM).In organizational project, polymer support, which has, connects good porous structure, they are Cell provides interim physical template, is convenient for cell adhesion, migration, proliferation, differentiation, neoblastic regeneration and three-dimensional structure.Most Eventually, bracket should be substituted by the natural extracellular matrix that cell is secreted.Biodegradable high polymer is that one kind has temptation very much Candidate material because they have structured integralities, last natural decomposition leaves the tissue newly formed, will undertake tissue Required mechanical load.Porous material is as stenter to implant animal or human body, it is necessary to have two functions, one is having one Fixed mechanical strength is to keep former organized shape;The second is induced cell growth and differentiation are had certain bioactivity, To guide the regeneration of damaged tissues, and timbering material can finally be degraded and absorbed.

Currently, preparing porous support materials there are many methods, mainly there is particle leaching, fibres bond, gas foaming, cold Dry, 3 D-printing and the mutually preparation methods such as separation is lyophilized.Though these preparation methods differ from one another but still have shortcoming.For example, Particle leaching is that soluble particle is added in the base as pore creating material, such as salt, sucrose, mannitol, sodium bicarbonate, phosphorus Acid dihydride sodium and sodium glutamate etc. are soaked into particular solution after matrix solidification, pore creating material therein are made to dissolve out and stay Lower hole.The method is simple and easy, and still, porous support materials mechanical strength made from this method is not high.

Summary of the invention

The present invention provides a kind of preparation method of functionalization composite porous material, what the preparation method in the present invention obtained Functionalization composite porous material mechanical strength with higher.

The present invention provides a kind of preparation method of functionalization composite porous material, comprising the following steps:

A pore creating material suspension) is laid in carrier surface, obtains the pore creating material template for being attached to carrier surface after dry;

B) Matrix Solution is taped against in the pore creating material template, obtains compound casting films after dry;

Described matrix solution includes basis material, carbon nanotube and solvent;

Described matrix material include one of polylactide glycolate copolymer, polylactic acid, fibroin albumen and chitosan or It is several;

C) the compound casting films are immersed in acid solution, obtain functionalization composite porous material.

Preferably, the pore creating material suspension is selected from melamine resin microsphere suspension liquid.

Preferably, the partial size of pore creating material is 100~10000nm in the pore creating material suspension.

Preferably, the mass concentration of the pore creating material suspension is 3~5%.

Preferably, the mass concentration of polylactide glycolate copolymer is (0.06~0.14) g/mL in described matrix solution.

Preferably, the mass ratio of the carbon nanotube and polylactide glycolate copolymer is (0.1~1): 100.

Preferably, the solvent is mixed solution, hexafluoroisopropanol or the chloroform of tetrahydrofuran and dimethylformamide;

The volume ratio of tetrahydrofuran and dimethylformamide in the mixed solution of the tetrahydrofuran and dimethylformamide For (1~5): 1.

Preferably, the polylactide glycolate copolymer and the volume ratio of pore creating material in pore creating material suspension are (2~3): 1。

Preferably, the step B) specifically:

Matrix Solution is taped against in pore creating material template, hygrometric state casting films are obtained, then vibrates the hygrometric state casting films, is done Compound casting films are obtained after dry.

Preferably, the time of the oscillation is 10~60min.

The present invention provides a kind of preparation methods of functionalization composite porous material, comprising the following steps: A) by pore creating material Suspension is laid in carrier surface, obtains the pore creating material template for being attached to carrier surface after dry;B Matrix Solution) is taped against institute It states in pore creating material template, obtains compound casting films after dry;Described matrix solution includes polylactide glycolate copolymer, carbon nanometer Pipe and solvent.C) the compound casting films are immersed in acid solution, obtain functionalization composite porous material.The present invention is by particle Leaching combines with curtain coating embrane method and prepares porous material, and porous material obtained can simulate extracellularly in form and function Matrix, and have good biocompatibility and good mechanical strength, the experimental results showed that, the preparation method system in the present invention The functionalization composite porous material tensile break stress obtained is 3.5~4.1MPa, and elongation at break is 75.16% or so.In addition, Preparation method preparation process in the present invention is simple, and pore size is easy to control the structural stability with good pattern.

Detailed description of the invention

In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis The attached drawing of offer obtains other attached drawings.

Fig. 1 is the scanning electron microscope (SEM) photograph for the PLGA/MWCNTs functionalization composite porous material that the embodiment of the present invention 1 obtains;

Fig. 2 is the scanning electron microscope (SEM) photograph for the PLGA/MWCNTs functionalization composite porous material that the embodiment of the present invention 2 obtains;

Fig. 3 is cell in the embodiment of the present invention 1, the PLGA/MWCNTs functionalization composite porous material of embodiment 2 and blank Adhesive tendencies on slide;

Fig. 4 is cell in the embodiment of the present invention 1, the PLGA/MWCNTs functionalization composite porous material of embodiment 2 and blank Proliferative conditions on slide;

Fig. 5 is that cell is adhered to and is proliferated on the PLGA/MWCNTs functionalization composite porous material in the embodiment of the present invention 2 Scanning electron microscope (SEM) photograph;

Fig. 6 is the scanning electron microscope (SEM) photograph for the PLGA/SWCNTs functionalization composite porous material that the embodiment of the present invention 3 obtains.

Specific embodiment

The present invention provides a kind of preparation methods of functionalization composite porous material, comprising the following steps:

A pore creating material suspension) is laid in carrier surface, obtains the pore creating material template for being attached to carrier surface after dry;

B) Matrix Solution is taped against in the pore creating material template, obtains compound casting films after dry;

Described matrix solution includes polylactide glycolate copolymer, carbon nanotube and solvent.

C) the compound casting films are immersed in acid solution, obtain functionalization composite porous material.

The functionalization composite porous material mechanical strength with higher that preparation method in the present invention obtains.

Pore creating material suspension is laid in carrier surface by the present invention, obtains the pore creating material mould for being attached to carrier surface after dry Plate, in the present invention, the pore creating material suspension are the unit for uniform suspension of pore creating material and water, and the pore creating material suspension preferably wraps Include melamine resin microsphere suspension liquid;The mass concentration of the pore creating material suspension is preferably 3~5%, more preferably 3.5~4.5%, most preferably 4%;The partial size of the pore creating material is preferably 100~10000nm, more preferably 1000~ 9000nm, specifically, in an embodiment of the present invention, can be 2 μm or 7 μm, as Berlin, Germany particle Co., Ltd provides Partial size is respectively 2 ± 0.06 μm, 7 ± 0.15 μm of melamine resin microballoon.

The present invention does not have special limitation to the type of the carrier, it is preferred to use the solid carrier of smooth surface, Such as glass slide.In the present invention, preferably 20~40 DEG C of the temperature of the drying, more preferably 25~35 DEG C;The drying Time is preferably 24~48 hours, and more preferably 30~40 hours;Obtained pore creating material template is substantially attached to glass slide Upper pore creating material microballoon layer, the pore creating material template can be single layer, be also possible to multilayer, depend on the needs.

After obtaining pore creating material template, Matrix Solution is taped against the pore creating material template surface by the present invention, is answered after dry Casting films are closed, Matrix Solution is preferably taped against in pore creating material template by the present invention, hygrometric state casting films is obtained, then by the hygrometric state stream Prolong film oscillation, obtains compound casting films after dry.It is to allow matrix molten to the purpose that the hygrometric state casting films carry out slight oscillatory Liquid is completely filled with the gap of the pore creating material template.The time of the oscillation is preferably 10~60min, more preferably 20~ 50min, most preferably 30~40min;The frequency of the oscillation is preferably 120~240 times/min, more preferably 150~200 Secondary/min.

In the present invention, described matrix solution includes basis material, carbon nanotube and solvent, and described matrix material includes poly- One or more of lactic acid glycolate copolymer (PLGA), polylactic acid (PLA), fibroin albumen and chitosan;Wherein, PLGA Molecular weight is 81000g/mol;Viscosity is 0.61dL/g.The carbon nanotube can be single-walled carbon nanotube (SWCNTs), can also To be multi-walled carbon nanotube (MWCNTs);The diameter of the carbon nanotube is preferably 30~70nm, more preferably 40~60nm, most Preferably 45~55nm;The length of the carbon nanotube is preferably 100~400nm, more preferably 150~350nm, most preferably 200~300nm.The mass concentration of polylactide glycolate copolymer is preferably (0.06~0.14) g/mL in described matrix solution, More preferably (0.08~0.12) g/mL, specifically, in an embodiment of the present invention, can be 0.06g/mL, 0.08g/mL, 0.10g/mL, 0.12g/mL or 0.14g/mL.The mass ratio of the carbon nanotube and polylactide glycolate copolymer is preferably (0.1~1): 100, more preferably (0.2~0.9): 100, specifically, in an embodiment of the present invention, can be 0.5:100. The solvent is preferably the mixed solution of tetrahydrofuran and dimethylformamide, the body of the tetrahydrofuran and dimethylformamide Product is than being preferably (1~5): 1, more preferably (2~4): 1, most preferably 3:1.

In the present invention, in the pore-creating and suspension the volume ratio of pore creating material and described matrix material be preferably 1:(2~ 3), more preferably 1:2.5.

The present invention preferably by polylactide glycolate copolymer while stirring, tetrahydrofuran and dimethyl formyl is added In the mixed organic solvents of amine, sufficiently dissolve, formed PLGA solution, then again by carbon nanotube under stirring conditions, uniformly It is dispersed in PLGA solution, obtains Matrix Solution, i.e. PLGA/CNTs solution.

In the present invention, the drying is preferably dried in vacuo, and the vacuum drying vacuum degree is preferably 0.02MPa; The vacuum drying time is preferably 24~48 hours;The vacuum drying temperature is preferably 20~40 DEG C.

After obtaining compound casting films, the present invention preferably immerses the compound casting films in acid solution, obtains functionalization Composite porous material.The acid solution preferably includes hydrochloric acid solution or sulfuric acid solution;The molar concentration of the acid solution is excellent It is selected as 0.5~2mol/L, more preferably 1mol/L, after the compound casting films are immersed acid solution, after 0.5~2 hour, is made Hole agent dissolution, carrier and compound casting films completely disengage, then the compound casting films of the disengaging are cleaned and are dried in vacuo, Obtain functionalization composite porous material.

In the present invention, the concentration of strict control acid solution and the dissolution time of pore creating material are needed, dissolution time is too long, holds Easily porous material is caused to deform, dissolution time is too short, keeps pore creating material dissolution insufficient, the pore creating material dissolution time in the present invention is excellent It is selected as 0.5~2 hour, more preferably 1~1.5 hour;Acid solution concentration is excessive, can destroy porous material, and concentration is too small, meeting Influence the dissolution of pore creating material.

According to being round, membranaceous functionalization composite porous material made from above-mentioned preparation method, thickness is preferably 0.001~0.1cm, more preferably 0.002~0.05cm.

Preparation method in the present invention has the advantage that

(1) PLGA/CNTs functionalization composite porous material is prepared using curtain coating embrane method and particle leaching, makes its mechanicalness It can be significantly improved;In addition the formation of material central hole structure is to dissolve out to realize by microballoon, and institute is especially suitable in this way Preferably prepare the lesser porous material of thickness;

(2) by using the microballoon of different-grain diameter, the controllable preparation to aperture of porous material is realized, hole pattern and structure are steady Qualitative good, porosity is 67%~70.1% or so;

(3) micro nano structure of PLGA/CNTs functionalization composite porous material have biggish specific surface area (0.2~ 0.5m2/ g), unique three-dimensional porous structure can promote the adherency and proliferation of cell.

In order to further illustrate the present invention, with reference to embodiments to a kind of compound porous material of functionalization provided by the invention Material is described in detail, but cannot be understood as limiting the scope of the present invention.

In the examples below, PLGA is purchased from Jinan Dai Gang biologic material products company, melamine resin microballoon (MF) suspension is what Berlin, Germany particle Co., Ltd provided, and microspherulite diameter is respectively 2 ± 0.06 μm, 7 ± 0.15 μm.

Porosity calculation formula:

In formula, m, r and h respectively represent the quality of perforated membrane, radius and thickness.ρbFor the bulk density of polymer, ε is hole Gap rate.

Embodiment 1

By the polylactide glycolate copolymer (PLGA) of 1g under stirring, it is added to tetrahydrofuran and dimethyl formyl Amine volume ratio is sufficiently to dissolve in the mixed organic solvents of 3:1, is made into the solution that concentration is 0.1g/mL;By 0.005g's MWCNTs is dispersed in PLGA solution, and PLGA/MWCNTs mixed solution is made.Measure the melamine that 5 μ L partial sizes are 2 μm Formaldehyde resin microballoon (MF) suspension is laid on sufficiently clean round slide, is completely dispersed and dries, obtain being attached to glass The MF microsphere template of on piece.The PLGA/MWCNTs mixed solution for measuring 10 μ L again is taped against on MF microsphere template, then will be mixed molten Liquid/MF template hygrometric state casting films slight oscillatory 30 minutes, it is therefore an objective to mixed solution be allowed to be completely filled between MF colloid template Gap is then dried in vacuo 48 hours;Finally, the slide for the compound PLGA/MWCNTs/MF casting films dried immerses 1mol/L's In hydrochloric acid solution, after 1 hour, the dissolution of MF microballoon, slide and PLGA/MWCNTs casting films are completely disengaged, sufficiently cleaning casting films, Then it is dried in vacuo to get the membranaceous PLGA/MWCNTs functionalization composite porous material of circle with a thickness of 0.003cm.

The porous material quality m that the present embodiment obtains is about 0.001g, and radius r is about 0.5cm, and thickness h is about 0.003cm, ρbFor 1.25g/cm3.It is calculated according to above-mentioned formula, the porosity of porous material in the present embodiment is 67%.

As shown in FIG. 1, FIG. 1 is of the invention real for PLGA/MWCNTs functionalization composite porous material pattern in the present embodiment Apply the scanning electron microscope (SEM) photograph for the PLGA/MWCNTs functionalization composite porous material that example 1 obtains.As seen from Figure 1, in the present embodiment The aperture of PLGA/MWCNTs functionalization composite porous material is 2 μm.

(Bei Shide instrument) is measured through specific surface instrument, PLGA/MWCNTs functionalization composite porous material in the present embodiment Specific surface area is 0.2~0.5m2/g。

The present invention tests PLGA/MWCNTs function in the present embodiment using universal testing machine (INSTRON 3365) Change the mechanical performance of composite porous material, tensile break stress 3.5MPa, elongation at break 60.5%.

Embodiment 2

By the polylactide glycolate copolymer (PLGA) of 1.2g under stirring, it is added to tetrahydrofuran and dimethyl methyl Amide volume ratio is sufficiently to dissolve in the mixed organic solvents of 3:1, is made into the solution that concentration is 0.12g/mL;By 0.012g's MWCNTs is dispersed in PLGA solution, and PLGA/MWCNTs mixed solution is made;Measure the melamine that 5 μ L partial sizes are 7 μm Formaldehyde resin microballoon (MF) suspension is laid on sufficiently clean round slide, is completely dispersed and dries, obtain being attached to glass The MF microsphere template of on piece.The PLGA/MWCNTs mixed solution for measuring 10 μ L again is taped against on MF microsphere template, then will be mixed molten Liquid/MF template hygrometric state casting films slight oscillatory 20 minutes, it is therefore an objective to mixed solution be allowed to be completely filled between MF colloid template Gap is then dried in vacuo 48 hours;Finally, the slide for the compound PLGA/MWCNTs/MF casting films dried immerses 1mol/L's In hydrochloric acid solution, after 1.5 hours, the dissolution of MF microballoon, slide and PLGA/MWCNTs casting films are completely disengaged, sufficiently cleaning curtain coating Then film is dried in vacuo to get the membranaceous PLGA/MWCNTs functionalization composite porous material of circle with a thickness of 0.0039cm.

The porous material quality m that the present embodiment obtains is about 0.0012g, and radius r is about 0.5cm, and thickness h is about 0.0039cm, ρbFor 1.25g/cm3.It is calculated according to above-mentioned formula, the porosity of porous material in the present embodiment is 69%.

PLGA/MWCNTs functionalization composite porous material pattern in the present embodiment is as shown in Fig. 2, Fig. 2 is that the present invention is real Apply the scanning electron microscope (SEM) photograph for the PLGA/MWCNTs functionalization composite porous material that example 2 obtains.As shown in Figure 2, in the present embodiment The aperture of PLGA/MWCNTs functionalization composite porous material is 7 μm.

(Bei Shide instrument) is measured through specific surface instrument, PLGA/MWCNTs functionalization composite porous material in the present embodiment Specific surface area is 0.05~0.1m2/g。

The present invention tests PLGA/MWCNTs function in the present embodiment using universal testing machine (INSTRON 3365) Change the mechanical performance of composite porous material, tensile break stress 4.1MPa, elongation at break 75.16%.

The PLGA/MWCNTs functionalization composite porous material and blank that Example 1 and Example 2 of the present invention is obtained Slide is put into 24 well culture plates, and 75% alcohol is added, soaking disinfection 4 hours, alcohol is then sucked out, dries, is impregnated with PBS solution Rinsing 3 times, every time 20 minutes.Then or so DMEM culture medium soak-out material half an hour is used, culture medium is then sucked, is put into CO2 After incubator a few hours, it is inoculated with according to 20000 cell densities in every hole.The cell of culture to certain time is taken from incubator Out, liquid-transfering gun sops up original culture solution, is rinsed 3 times with the PBS solution by preheating, every hole be added be preheating to 37 DEG C not added with 10% fetal calf serum and dual anti-360 μ L of DMEM culture medium, addition are preheating to 37 DEG C of 40 μ L of MTT, cultivate 4 hours, will there is purple Color precipitating generates, and then culture medium is sucked, and 400 μ L DMSO are added in every hole, and dissolution precipitating 20 minutes becomes purple solution, so It pipettes 100 μ L of solution with liquid-transfering gun afterwards to be added in every hole of 96 well culture plates, the Detection wavelength in Multifunction fluorescent microplate reader Fluorescent value at 570nm, what size can reflect living cells sticks trend.

As a result as seen in figures 3-5, Fig. 3 is that adherency of the cell on the embodiment of the present invention 1, embodiment 2 and blank slide becomes Gesture, wherein 1 is adhesive tendencies of the cell on blank slide, and 2 be PLGA/MWCNTs function of the cell in the embodiment of the present invention 1 The adhesive tendencies on composite porous material can be changed, 3 is compound for PLGA/MWCNTs functionalization of the cell in the embodiment of the present invention 2 Adhesive tendencies on porous material.As seen from Figure 3, with the extension of time, compared with blank slide, cell is in porous material Sticking on material is more and more;Fig. 4 is that cell is compound more in the embodiment of the present invention 1, the PLGA/MWCNTs functionalization of embodiment 2 Proliferative conditions on Porous materials and blank slide, wherein 1 is proliferative conditions of the cell on blank slide, and 2 be the cell present invention The proliferative conditions on PLGA/MWCNTs functionalization composite porous material in embodiment 1,3 is in the cell embodiment of the present invention 2 Proliferative conditions on PLGA/MWCNTs functionalization composite porous material.As seen from Figure 4, with the increase of cultivated days, with Blank slide is compared, and the proliferative conditions of cell on the porous material are good.Fig. 5 is PLGA/ of the cell in the embodiment of the present invention 2 The scanning electron microscope (SEM) photograph for adhering to and being proliferated on MWCNTs functionalization composite porous material.

Embodiment 3

By the polylactide glycolate copolymer (PLGA) of 1g under stirring, it is added to tetrahydrofuran and dimethyl formyl Amine volume ratio is sufficiently to dissolve in the mixed organic solvents of 3:1, is made into the solution that concentration is 0.1g/mL;By 0.01g's SWCNTs is dispersed in PLGA solution, and PLGA/SWCNTs mixed solution is made;Measure the melamine that 5 μ L partial sizes are 7 μm Formaldehyde resin microballoon (MF) suspension is laid on sufficiently clean round slide, is completely dispersed and dries, obtain being attached to glass The MF microsphere template of on piece.The PLGA/SWCNTs mixed solution for measuring 10 μ L again is taped against on MF microsphere template, then will be mixed molten Liquid/MF template hygrometric state casting films slight oscillatory 20 minutes, it is therefore an objective to mixed solution be allowed to be completely filled between MF colloid template Gap is then dried in vacuo 48 hours;Finally, the slide for the compound PLGA/SWCNTs/MF casting films dried immerses 1mol/L's In hydrochloric acid solution, after 1.5 hours, the dissolution of MF microballoon, slide and PLGA/MWCNTs casting films are completely disengaged, sufficiently cleaning curtain coating Then film is dried in vacuo to get the membranaceous PLGA/MWCNTs functionalization composite porous material of circle with a thickness of 0.0034cm.

Porous material quality m in the present embodiment is about 0.001g, and radius r is about 0.5cm, and thickness h is about 0.0034cm, ρbFor 1.25g/cm3.It is computed, the porosity of porous material in the present embodiment is 70.1%.

PLGA/SWCNTs functionalization composite porous material pattern in the present embodiment is as shown in fig. 6, Fig. 6 is that the present invention is real Apply the scanning electron microscope (SEM) photograph for the PLGA/SWCNTs functionalization composite porous material that example 3 obtains.It will be appreciated from fig. 6 that in the present embodiment The aperture of PLGA/SWCNTs functionalization composite porous material is 7 μm.

(Bei Shide instrument) is measured through specific surface instrument, PLGA/SWCNTs functionalization composite porous material in the present embodiment Specific surface area is 0.1~0.3m2/g。

The present invention tests PLGA/SWCNTs function in the present embodiment using universal testing machine (INSTRON 3365) Change the mechanical performance of composite porous material, tensile break stress 3.8MPa, elongation at break 69.3%.

The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (8)

1. a kind of preparation method of functionalization composite porous material, comprising the following steps:
A pore creating material suspension) is laid in carrier surface, obtains the pore creating material template for being attached to carrier surface after dry;
B) Matrix Solution is taped against in the pore creating material template, obtains compound casting films after dry;
Described matrix solution includes polylactide glycolate copolymer, carbon nanotube and solvent;
The mass concentration of polylactide glycolate copolymer is (0.1~0.12) g/mL in described matrix solution;
The mass ratio of the carbon nanotube and polylactide glycolate copolymer is (0.5~1): 100;
C) the compound casting films are immersed in the hydrochloric acid solution of 1mol/L, obtain functionalization composite porous material.
2. preparation method according to claim 1, which is characterized in that the pore creating material suspension is selected from melamino-formaldehyde Resin microsphere suspension.
3. preparation method according to claim 2, which is characterized in that the partial size of pore creating material is in the pore creating material suspension 100~10000nm.
4. preparation method according to claim 1, which is characterized in that the mass concentration of the pore creating material suspension be 3~ 5%.
5. preparation method according to claim 1, which is characterized in that the solvent is tetrahydrofuran and dimethylformamide Mixed solution, hexafluoroisopropanol or chloroform;
The volume ratio of tetrahydrofuran and dimethylformamide is (1 in the mixed solution of the tetrahydrofuran and dimethylformamide ~5): 1.
6. preparation method according to claim 1, which is characterized in that the polylactide glycolate copolymer and pore creating material are outstanding The volume ratio of pore creating material is (2~3) in supernatant liquid: 1.
7. preparation method described in any one according to claim 1~6, which is characterized in that the step B) specifically:
Matrix Solution is taped against in pore creating material template, hygrometric state casting films are obtained, then vibrates the hygrometric state casting films, after dry Obtain compound casting films.
8. preparation method according to claim 7, which is characterized in that the time of the oscillation is 10~60min.
CN201610300686.5A 2016-05-09 2016-05-09 A kind of preparation method of functionalization composite porous material CN105837849B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101979103A (en) * 2010-10-26 2011-02-23 中南大学 Method for preparing porous tissue engineering scaffold
CN103638561A (en) * 2013-11-29 2014-03-19 苏州大学 Preparation method of micro-nano bioactive porous material

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7713778B2 (en) * 2003-02-13 2010-05-11 Univ California Nanostructured casting of organic and bio-polymers in porous silicon templates
US20090114089A1 (en) * 2007-11-02 2009-05-07 Chunqing Liu Microporous Aluminophosphate Molecular Sieve Membranes for Highly Selective Separations

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101979103A (en) * 2010-10-26 2011-02-23 中南大学 Method for preparing porous tissue engineering scaffold
CN103638561A (en) * 2013-11-29 2014-03-19 苏州大学 Preparation method of micro-nano bioactive porous material

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