CN105837837B - A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver - Google Patents
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver Download PDFInfo
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Abstract
The preparation method of the invention discloses a kind of medical chitosan temperature-sensitive hydrogel containing nano silver.Products obtained therefrom of the present invention is a kind of novel medical material.The temperature-sensitive hydrogel, using staphylococcus aureus as the gram-positive bacteria of representative, to using Candida albicans as the fungi of representative, all has preferable bacteriostasis, has a broad antifungal spectrum, no cytotoxicity, high-efficiency low-toxicity to using Escherichia coli as the Gram-negative bacteria of representative；Uniformly layer of gel film can be formed in wound surface after sprinkling, bacteriostasis persistently, gas permeability and hygroscopicity it is good, have the function of wound healing, hemostasis, analgesic, anti-inflammatory sterilization, it can be used for burning, scald, the irregular wound of large area such as wound, hemostasis, analgesic rapidly, and prevention infection, wound healing, it can also be used to other scytitis such as bedsore, skin ulcer, tinea of feet and hands, women vaginitis, cervicitis, cervical erosion.
Description
Technical field
The present invention relates to medical material fields, more particularly to a kind of medical chitosan temperature-sensitive hydrogel containing nano silver
Preparation method.
Background technique
Chitosan is the unique a large amount of existing alkaline polysaccharides of nature, is a kind of sun that chitin is formed after deacetylated
Nonionic polysaccharide.Chitosan is widely present in the cell wall of the shell of arthropod, fungi and yeast, and stock number is very rich,
There are the characteristics such as biodegradable, nontoxic, antibacterial simultaneously, there is good biocompatibility, deer horn glue-chitosan cytoskeleton
The enhancing of bone cell proliferation vigor, carboxymethyl chitosan can be promoted to have facilitation, basilar memebrane base to the proliferation secretion of schwann cell
Matter/chitosan inducing bone mesenchymal stem cell is at cartilage differentiation etc..There is a large amount of hydroxyl and amino, easily in chitosan molecule
It is modified by sulphation, complex can be formed in conjunction with metal ion, form a series of new chitosan derivatives, be provided with more
Biological function, therefore obtained in-depth study in medicine, field of biology and be widely applied, Transforming growth factor-β3
With the compound fibroin albumen of -2 co-transfection bone marrow mesenchymal stem cells of Matrix Metalloproteinase Inhibitors/chitosan biological bracket
Rabbit cartilage defect is repaired, is a kind of ideal medical material.
Silver ion in early stage just since its excellent antibiotic and sterilizing performance is by people institute extensive concern, medical material with
The fields such as anti-biotic material have broad application prospects.There is no apparent cell poly- with the cell after low concentration silver ion treatment
The variation of collection, cytomorphosis, cell dissolution etc., it can be seen that a small amount of silver ion does not have apparent harmful effect to human body, together
Shi Yin has good bactericidal effect.Nano silver is the novel antibacterial product developed based on nanotechnology, is had than Ag+
There is more stable physicochemical characteristics, nano silver is not likely to produce drug resistance due to quantum effect, small-size effect, and nano silver is anti-
Bacterium mechanism of action: dose dependent promotes bacterium apoptosis, accelerates the healing of wound, has in the fields such as medical material and anti-biotic material
Have broad application prospects.
Temperature sensitive type water gel refers to the variation with ambient temperature and generates the intellectual material of stimulating responsive.Temperature
There is a certain proportion of hydrophilic and hydrophobic grouping, the variation of temperature can influence dredging for these groups in sensitive hydrogel structure
Hydrogen bond action between water effect and macromolecular chain, to influence its metamorphosis.The most common temperature-sensitive hydrogel is poly- N-
Isopropylacrylamide hydrogel, existing hydrophobic grouping has hydrophilic radical again in the system of this hydrogel, these groups can be with
It acts on hydrone, also has an effect with hydrone outside molecule in the molecule.Compared with synthesizing macromolecule, natural polymer is solidifying
Glue has many advantages, such as hypotoxicity, biocompatibility and degradability.Thermo-sensitive aquagel can be according to temperature change
Different physical aspects.Under room temperature be liquid, into after tissue, body temperature effect under gelation and be fixed on local organization
In.Chitosan only keeps solution state under acidic environment, and when pH value is increased to 6.2, chitosan solution starts to sink
It forms sediment, if sodium glycero-phosphate is added, which can keep solution state in neutral conditions, which has temperature sensitivity,
Solution state is kept below body temperature, and gel can be formed when temperature is increased to body temperature.When it has good biocompatibility, in vivo degradation
Between and setting time the advantages that being adjustably controlled, lead bioengineering, field of medicaments to obtain extensive concern in engineering etc..
105037820 A(application number 201510412714.8 of application publication number CN) Chinese patent literature disclose one
Kind can form chitosan/oxidized graphene/R- phosphoglycerol disodium temperature-sensitive hydrogel of gel under the conditions of being lower than 37 DEG C, pass through
Rheometer test have studied chitosan concentration, two na concn of R- phosphoglycerol and graphene oxide concentration to aquagel at
The influence of glue temperature and gelation time.105288700 A(application number 201510847097.4 of application publication number CN) China specially
Sharp document discloses a kind of medical temperature-sensitive hydrogel dressing and preparation method thereof, medical temperature-sensitive hydrogel dressing, including with
The raw material components of lower weight percent: 2-3% chitosan, 6-9% hydroxypropyl methyl cellulose, 50-60% glycerol, 1-2% acetic acid,
0.5-1% sodium carbonate, surplus are water.104984401 A(application number 201510434128.3 of application publication number CN) China specially
Sharp document discloses a kind of preparation method of temperature-sensitive hydrogel/tricalcium phosphate material, and this method steps flow chart includes by temperature sensitive water
Gel dissolves at low temperature, tricalcium phosphate powder, the random moulding of mixing material is added by a certain percentage into any desired shape.
Currently, using chitosan as carrier, dispersing agent and protectant temperature sensitive water-setting of the medical chitosan containing nano silver
The preparation method and application of glue has not been reported.
Summary of the invention
In view of the deficiencies of the prior art, an object of the present invention is to provide a kind of medical chitosan temperature containing nano silver
The preparation method of quick hydrogel.Medical chitosan temperature-sensitive hydrogel of the gained containing nano silver of the invention is at room temperature can
Colloidal solution is flowed, and is changed into immobilising gel under the conditions of 37 DEG C, is a kind of novel medical material.The temperature sensitive water-setting
Glue is to using Escherichia coli as the Gram-negative bacteria of representative, using staphylococcus aureus as the gram-positive bacteria of representative, to
Candida albicans is the fungi of representative, all has preferable bacteriostasis, has a broad antifungal spectrum, no cytotoxicity, high-efficiency low-toxicity；Uniformly
After sprinkling can wound surface formed layer of gel film, bacteriostasis persistently, gas permeability and hygroscopicity it is good, have promote wound
Face healing, hemostasis, analgesic, anti-inflammatory sterilization effect, can be used for burning, scald, the irregular wound of large area such as wound, rapidly only
Blood, analgesic, and prevent infection, wound healing, it can also be used to bedsore, skin ulcer, tinea of feet and hands, women vaginitis, uterine neck
Other scytitis such as inflammation, cervical erosion.
The second object of the present invention is to provide above-mentioned temperature-sensitive hydrogel treatment burn, scald, wound, bedsore, skin ulcer,
The application in drug that tinea of feet and hands, women vaginitis, cervicitis, cervical erosion, reduction scar generate.
The preparation method of the above-mentioned medical chitosan temperature-sensitive hydrogel containing nano silver is:
(1) chitosan and silver nitrate are ground into fine powder respectively, mixed, the mass ratio of chitosan and silver nitrate is 100:
0.002-0.1 sufficiently quickly grinds 1.5-4 hours, ultraviolet light 4-20 hours, obtains chitosan nano silver composite；
(2) by chitosan nano silver composite obtained by step (1) with concentration be 0.1-0.25mol/L aqueous acetic acid it is molten
Solution is configured to the chitosan nano silver complex solution that mass concentration is 1-2.5%；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:1-10:3, is quickly stirred,
The disodium hydrogen phosphate buffer of saturation is added dropwise into resulting solution, is quickly stirred when being added, until pH to 6.82-
7.00；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after a certain period of time, by can
The colloidal solution of flowing is changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
It is preferred that being step (1), the chitosan is purchased from Zhejiang Province gold shell Biochemie Co., Ltd, emerging biological section of Zhejiang Australia
Skill Co., Ltd or Shandong Jia Feng marine organisms Science and Technology Ltd..
It is preferred that being step (1), the mass ratio of chitosan and silver nitrate is 100:0.005-0.05.
It is preferred that being step (1), sufficiently quickly grind 1.8-3.5 hours.
It is preferred that it is step (1), ultraviolet light 6-18 hours.
It with concentration is 0.12-0.2mol/L by chitosan nano silver composite obtained by step (1) it is preferred that be step (2)
Aqueous acetic acid dissolution.
It is preferred that being step (2), it is configured to the chitosan nano silver complex solution that mass concentration is 1.5-2.2%.
It is preferred that being step (3), the volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:
1.5-10:2.5.
It is preferred that being step (3), until pH to 6.85-6.95.
More preferred, step (1) chitosan is purchased from Zhejiang Province gold shell Biochemie Co., Ltd.
More preferred, the mass ratio of step (1) chitosan and silver nitrate is 100:0.02.
More preferred, step (1) is sufficiently quickly ground 2.5 hours.
More preferred, step (1) ultraviolet light 16 hours.
More preferred, chitosan nano silver composite obtained by step (1) is 0.16mol/L's with concentration by step (2)
Aqueous acetic acid dissolution.
More preferred, chitosan nano silver composite obtained by step (1) is 0.16mol/L's with concentration by step (2)
Aqueous acetic acid dissolution.
More preferred, step (3) chitosan nano silver complex solution and the volume ratio of sodium β-glycerophosphate solution are
10:2.
More preferred, step (3) is until pH to 6.9.
The present invention also provides the medical chitosan temperature-sensitive hydrogel containing nano silver biomedical aspect application, especially
Its application in terms of biocidal property.
Chitosan nano silver composite is made with solid phase method in the present invention, and contains the medical of nano silver as raw material preparation
Chitosan temperature-sensitive hydrogel has the function of antibacterial, promotion wound healing, reduces scar generation, hemostasis, and has good guarantor
Wet, gas permeability, no cytotoxicity, good antimicrobial effect, persistent.The present invention can also reduce the use of antibiotic, and make
Standby simple process, has broad application prospects.In addition to this excellent effect of the invention is also manifested as follows:
1, the characteristics of having both nano silver and chitosan and advantage not only have the antibiotic property of nano silver, are also equipped with chitosan
Promote cell growth, reduces the effect that scar generates；
2, chitosan nano silver composite is made by carrier of chitosan during preparation, chitosan is as nano silver
Dispersing agent and protective agent, make nano silver is uniform and stable to be distributed in temperature-sensitive hydrogel system.
3, present invention medical chitosan temperature-sensitive hydrogel of the gained containing nano silver is to blue by the leather of representative of Escherichia coli
Family name's negative bacterium, to using Candida albicans as the fungi of representative, has using staphylococcus aureus as the gram-positive bacteria of representative
There is preferable contact bacteriostasis.
4, medical chitosan temperature-sensitive hydrogel of the present invention gained containing nano silver can preferably prevent trauma surface infestation, reduce
Scar generates, while having good moisturizing, gas permeability, integrates multi-functional.
Specific embodiment
The technical solution of the present invention will be described in detail below with reference to the embodiments and experimental examples, but protection scope is without being limited thereto.
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver of embodiment 1:
(1) by chitosan (chitosan used in the present embodiment is purchased from Zhejiang ZHEJIANG AOXING BIOTECHNOLOGY CO., LTD) and nitric acid
Silver is ground into fine powder respectively, mixes, and the mass ratio of chitosan and silver nitrate is 100:0.002, sufficiently quick grinding 1.5 hours, purple
Outer light irradiates 4 hours, obtains chitosan nano silver composite；
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.1mol/L, is matched
The chitosan nano silver complex solution that mass concentration is 1% is made；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:1, is quickly stirred, to institute
The disodium hydrogen phosphate buffer of saturation is added dropwise in the solution obtained, is quickly stirred when being added, until pH to 6.82；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after 5min, by flowable
Colloidal solution be changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver of embodiment 2:
(1) by chitosan (chitosan used in the present embodiment is purchased from Zhejiang ZHEJIANG AOXING BIOTECHNOLOGY CO., LTD) and nitric acid
Silver is ground into fine powder respectively, mixes, and the mass ratio of chitosan and silver nitrate is 100:0.1, sufficiently quick grinding 4 hours, ultraviolet light
Irradiation 20 hours, obtains chitosan nano silver composite；
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.25mol/L,
It is configured to the chitosan nano silver complex solution that mass concentration is 2.5%；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:3, is quickly stirred, to institute
The disodium hydrogen phosphate buffer of saturation is added dropwise in the solution obtained, is quickly stirred when being added, until pH to 7.00；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after 4.5min, by that can flow
Dynamic colloidal solution is changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver of embodiment 3:
(1) chitosan (being purchased from Shandong Jia Feng marine organisms Science and Technology Ltd.) is ground into fine powder with silver nitrate respectively, is mixed
It closes, the mass ratio of chitosan and silver nitrate is 100:0.005, sufficiently quickly grinds 1.8 hours, ultraviolet light 6 hours, obtains shell
Glycan nano-ag composite；
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.12mol/L,
It is configured to the chitosan nano silver complex solution that mass concentration is 1.5%；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:1.5, is quickly stirred, to
The disodium hydrogen phosphate buffer of saturation is added dropwise in resulting solution, is quickly stirred when being added, until pH to 6.85；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after 4min, by flowable
Colloidal solution be changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver of embodiment 4:
(1) chitosan (being purchased from Shandong Jia Feng marine organisms Science and Technology Ltd.) is ground into fine powder with silver nitrate respectively, is mixed
It closes, the mass ratio of chitosan and silver nitrate is 100:0.05, sufficiently quickly grinds 3.5 hours, ultraviolet light 18 hours, obtains shell
Glycan nano-ag composite；
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.2mol/L, is matched
The chitosan nano silver complex solution that mass concentration is 2.2% is made；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:2.5, is quickly stirred, to
The disodium hydrogen phosphate buffer of saturation is added dropwise in resulting solution, is quickly stirred when being added, until pH to 6.95；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after 3min, by flowable
Colloidal solution be changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
A kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver of embodiment 5:
(1) chitosan (being purchased from Zhejiang Province gold shell Biochemie Co., Ltd) is ground into fine powder with silver nitrate respectively, is mixed, shell
The mass ratio of glycan and silver nitrate is 100:0.02, sufficiently quickly grinds 2.5 hours, ultraviolet light 16 hours, obtains chitosan
Nano-ag composite；
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.16mol/L,
It is configured to the chitosan nano silver complex solution that mass concentration is 1.8%；
(3) 56% sodium β-glycerophosphate solution is added into chitosan nano silver complex solution obtained by step (2),
The volume ratio of chitosan nano silver complex solution and sodium β-glycerophosphate solution is 10:2, is quickly stirred, to institute
The disodium hydrogen phosphate buffer of saturation is added dropwise in the solution obtained, is quickly stirred when being added, until pH to 6.9；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after 2.5min, by that can flow
Dynamic colloidal solution is changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
The bacteriostasis research of 1 medical chitosan temperature-sensitive hydrogel of the present invention gained containing nano silver of test example
Bacterium: G+ bacteria (G+): S. atreus clinical bacterium (offer of the Liaocheng City hospital of traditional Chinese hospital), gold
Staphylococcus aureus reference culture ATCC25923(Liaocheng People's Hospital provides).
Gram-negative bacterium (G-): Escherichia coli clinical strains, pneumoniae clinical bacterial strain, cloaca intestines bar
Bacterium clinical strains, streptococcus pneumonia clinical strains, P. aeruginosa clinical bacterial strain (being provided by the Liaocheng City hospital of traditional Chinese hospital)；Greatly
Enterobacteria reference culture ATCC25922, pseudomonas aeruginosa reference culture ATCC27853, haemophilus influenzae clinical strains are (
It is provided by Liaocheng People's Hospital).
Fungi: Candida albicans, Candida glabrata, candida krusei (being provided by the Liaocheng City hospital of traditional Chinese hospital).
Each strain of preservation is subjected to activation culture respectively, after growing single colonie, with a little thallus of oese picking in dress
In the test tube for having 9mL sterile saline, shaken well prepares 10 respectively7 The suspension of cfu/mL.Aseptically, exist
Add suitable culture medium in the culture dish of 90mm, after solidification plus 100 microlitres of bacterium solutions, coating uniformly, stand 20-30min.It will preparation
Temperature-sensitive hydrogel sample be made into respectively diameter be 0.4cm circle, be placed on solid medium, on same culture dish successively
The embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 are placed, is repeated 6 times, in 37 DEG C of constant incubator
Middle culture is for 24 hours；Whether there is or not bacterial growths for the contact surface of observation sample and culture medium, and measure the size of inhibition zone, using SPSS
The analysis of v11.5 software statistics, data are indicated with mean ± standard deviation (x ± S).
Bacteriostasis of each sample to 3 kinds of fungies
Each strain of preservation is subjected to activation culture respectively, preparing concentration respectively is 1 × 106The bacterium solution of cfu/mL, it is other
Operation is same as above, and cultivates 48h in 37 DEG C of constant incubator, and observing the contact surface of sample and culture medium, whether there is or not bacterial growth, measurements
The size of inhibition zone.It is analyzed using SPSS software statistics, measurement data is indicated with mean ± standard deviation (x ± S).
Temperature-sensitive hydrogel containing nano silver is to 10 kinds of bacteriums: Escherichia coli clinical strains and its reference culture ATCC25922,
S. atreus clinical bacterial strain and its reference culture ATCC25923, P. aeruginosa clinical bacterial strain and its reference culture
ATCC27853, enterobacter cloacae, streptococcus pneumonia, haemophilus influenzae, Klebsiella oxytoca antibacterial circle diameter are shown in Table 1.
Table 1: products obtained therefrom of the present invention is to the contact of 10 kinds of bacteriums antibacterial antibacterial circle diameter (unit: cm；Sample diameter
0.4cm)
As can be seen from Table 1, products obtained therefrom of the present invention to the contact bacteriostatic experiment for testing 10 kinds of bacteriums used the result shows that:
Products obtained therefrom of the present invention, which has, preferably contacts bacteriostasis, and the contact surface of sample and culture medium is without bacterial growth.
Products obtained therefrom of the present invention is to three kinds of fungies: the antibacterial suppression of contact of Candida albicans, Candida glabrata, candida krusei
Bacterium loop diameter is shown in Table 2.
Table 2: each sample is to the contact of three kinds of fungies antibacterial antibacterial circle diameter (unit: cm；Sample diameter 0.4cm)
As can be seen from Table 2, products obtained therefrom of the present invention all has experiment three kinds of fungies used and preferably contacts antibacterial work
With the contact surface of gel sample and culture medium is grown without fungi.Antibacterial work of the products obtained therefrom of the present invention to Candida albicans
With most obvious, followed by Candida glabrata is markedly less than first two fungi to the bacteriostasis of candida krusei.
Experimental result is shown: products obtained therefrom of the present invention is to using Escherichia coli as the Gram-negative bacteria of representative, to golden yellow
Color staphylococcus is the gram-positive bacteria of representative, is pressed down to preferable contact using Candida albicans as the fungi of representative, is all had
Bacterium effect.Staphylococcus aureus, Escherichia coli, Candida albicans are surgery, skin and gynecological infection (such as vaginitis, uterine neck
Inflammation, cervical erosion, bedsore, skin ulcer, burn, scald, wound, scytitis, tinea of feet and hands) common causative strain.
Bath temperature is shown in Table 3 to gelation influence.
Table 3: bath temperature influences gelation
As can be seen from Table 3,37 DEG C of transition temperatures for products obtained therefrom of the present invention, when being lower than 37 DEG C, product will not be sent out
Raw sol-gel transition, just can be changed into immobilising hydrogel from flowable colloidal solution when being higher than 37 DEG C.
Finally it should be noted that embodiment is the optimal specific embodiment of the present invention, it is not limited to this
Invention, although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, according to
It is so possible to modify the technical solutions described in the foregoing embodiments, or part of technical characteristic is equally replaced
It changes.All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention
Protection scope within.
Claims (8)
1. a kind of preparation method of the medical chitosan temperature-sensitive hydrogel containing nano silver, step
(1) chitosan and silver nitrate are ground into fine powder respectively, mixed, the mass ratio of chitosan and silver nitrate is 100:0.005-
0.05, it sufficiently quickly grinds 1.8-3.5 hours, ultraviolet light 6-18 hours, obtains chitosan nano silver composite；Its feature exists
In step (2)-(4) are as follows:
(2) chitosan nano silver composite obtained by step (1) is dissolved with the aqueous acetic acid that concentration is 0.12-0.2mol/L,
It is configured to the chitosan nano silver complex solution that mass concentration is 1.8%；
(3) 56% sodium β-glycerophosphate solution, chitosan are added into chitosan nano silver complex solution obtained by step (2)
The volume ratio of nano-ag composite solution and sodium β-glycerophosphate solution is 10:1.5-10:2.5, is quickly stirred, to resulting molten
The disodium hydrogen phosphate buffer of saturation is added dropwise in liquid, is quickly stirred when being added, until pH to 6.85-6.95；
(4) step (3) acquired solution is put into 37 DEG C of thermostat water baths, phase transition occurs after a certain period of time, by flowable
Colloidal solution be changed into immobilising hydrogel to get the medical chitosan temperature-sensitive hydrogel containing nano silver.
2. preparation method as described in claim 1, which is characterized in that the mass ratio of step (1) chitosan and silver nitrate is
100:0.02.
3. preparation method as described in claim 1, which is characterized in that step (1) is sufficiently quickly ground 2.5 hours.
4. preparation method as described in claim 1, which is characterized in that step (1) ultraviolet light 16 hours.
5. preparation method as described in claim 1, which is characterized in that step (2): by chitosan nano silver obtained by step (1)
Compound is dissolved with the aqueous acetic acid that concentration is 0.16mol/L.
6. preparation method as described in claim 1, which is characterized in that step (3): chitosan nano silver complex solution and β-
The volume ratio of phosphoglycerol sodium solution is 10:2.
7. preparation method as described in claim 1, which is characterized in that step (3): until pH to 6.9.
It burns, scald 8. medical chitosan temperature-sensitive hydrogel obtained by preparation method as described in claim 1 is treated in preparation
Wound, bedsore, skin ulcer, tinea of feet and hands, women vaginitis, cervicitis, cervical erosion, is reduced in the drug that scar generates wound
Application.
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| CN108727833B (en) * | 2017-12-07 | 2020-09-18 | 西南交通大学 | Preparation method of adhesive super-strong antibacterial hydrogel for bone/cartilage repair |
| CN111067862B (en) * | 2019-12-25 | 2021-03-30 | 山西大学 | Temperature-sensitive hydrogel with visual diagnosis and infection wound treatment functions and preparation method thereof |
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| CN1593385A (en) * | 2004-06-22 | 2005-03-16 | 天津大学 | Gel capable of injecting temperature sensitive complex and its preparation method |
| CN101176789A (en) * | 2006-11-11 | 2008-05-14 | 中国海洋大学 | Method for preparing temperature sensitive sustained-release gel of chitosan fat complexes |
| CN102358787A (en) * | 2009-08-11 | 2012-02-22 | 聊城大学 | Solid phase synthesis method of silver and chitosan and/or chitosan derivative nanometer compound |
| CN102399378A (en) * | 2010-09-07 | 2012-04-04 | 中国人民解放军总医院 | Temperature sensitive chitosan hydrogel and its preparation method |
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| CN101176789A (en) * | 2006-11-11 | 2008-05-14 | 中国海洋大学 | Method for preparing temperature sensitive sustained-release gel of chitosan fat complexes |
| CN102358787A (en) * | 2009-08-11 | 2012-02-22 | 聊城大学 | Solid phase synthesis method of silver and chitosan and/or chitosan derivative nanometer compound |
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