CN105831387A - Chinese chestnut-kudzu vine root composite peptide powder and preparing method thereof - Google Patents
Chinese chestnut-kudzu vine root composite peptide powder and preparing method thereof Download PDFInfo
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- CN105831387A CN105831387A CN201610214810.6A CN201610214810A CN105831387A CN 105831387 A CN105831387 A CN 105831387A CN 201610214810 A CN201610214810 A CN 201610214810A CN 105831387 A CN105831387 A CN 105831387A
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- Prior art keywords
- powder
- semen castaneae
- radix puerariae
- kudzu vine
- chinese chestnut
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
Chinese chestnut-kudzu vine root composite peptide powder is prepared from Chinese chestnut and kudzu vine root. The weight ratio of the Chinese chestnut to the kudzu vine root is 1-99:1-99. Through combination of the Chinese chestnut and the kudzu vine root, the composite peptide powder is high in nutrient contents, rich in trace elements, and capable of promoting human body metabolism, strengthening brain, improving intelligence, preventing cardiovascular and cerebrovascular diseases and facilitating recovery from constipation, chronic enteritis and other diseases, and has effects of protecting health and prolonging life. According to a peptide liquid prepared through a method mentioned above, effective components in the raw materials are fully extracted, active compounds in the raw materials are preserved to the utmost, comprehensive absorption of a human body is facilitated, a process is advanced and reasonable and quality is controllable.
Description
Technical field
The present invention relates to technical field of bioengineering, be specifically related to a kind of Semen Castaneae, Radix Puerariae complex peptides powder and
Preparation method.
Background technology
Semen Castaneae, is also called Semen Castaneae, hair Semen Castaneae etc., belongs to perennial plant, and it contains abundant nutrient
Matter, in every 100 grams starch-containing 51～60%, protein 5.7～10.7%, fat 2～7.4%, calcium 15 milligrams,
Ferrum 1.2 milligrams, 1.32 milligrams of zinc, vitamin e1 1.45 milligrams, 0.15 milligram of riboflavin, Catergen .5
The mineral such as milligram, crude fibre, carotene, vitamin A, B, C and calcium, phosphorus, potassium, is available for human body
The nutrient absorbed and utilize is up to 98%.Its content of starch is the highest, and the carbohydrate of dry plate chestnut reaches
77%, suitable with the 75% of Cereals class；It it is 2.4 times of Rhizoma Solani tuber osi.The vitamin B1 of Semen Castaneae, B2 rich content,
The content of vitamin B2 is at least 4 times of rice, every 100 grams possibly together with 24 milligrams of vitamin Cs, this is grain
Can not compare.Vitamin C contained by fresh Semen Castaneae contains more than the Fructus Lycopersici esculenti that vitamin C is abundant than generally acknowledging,
More than ten times of Fructus Mali pumilae especially.Semen Castaneae is sweet in flavor and cold in property, has the function of nourishing stomach and spleen, Kidney-invigorating and tendon-reinforcing.Semen Castaneae institute
Can provide high heat containing high starchiness, and potassium helps maintenance normal heartbeat rule, cellulose then can strengthen intestinal
Road, keeps Excretory system normal operation.Auxiliary treatment is suffered from a deficiency of the kidney useful by Semen Castaneae, therefore also known as " kidney fruit ".
Although Semen Castaneae has the highest nutritive value, but due to its storage difficulty, contains substantial amounts of in addition
Starch, current people are confined to fry outside the roughing food such as Semen Castaneae, Chinese chestnut sauce with it as canned food, sugar,
It is merely able to seasonal edible for people, and can not the most well develop the nutrition that Semen Castaneae is abundant
It is worth, its added value can not be improved.
Radix Puerariae is a kind of wild plant, and containing multiple flavones ingredient, main active is Dai, big
Bean glycoside, puerarin, puerarin-7-xyloside etc..Radix Puerariae is good, gas is flat, sweet in the mouth, have heat clearing away, fall
Fire, all effects of toxin expelling, modern medicine study shows: flavone compound puerarin is to hypertension, high blood
Fat, hyperglycemia and obesity and cardiovascular and cerebrovascular disease have certain curative effect.Its tradition powder of Radix Puerariae, molecular weight be big,
It is difficult to after people is edible absorb and utilize, because its molecular weight is little, effective ingredient easily absorbs profit after making kudzu root peptides
With, eating effect can be strengthened, play physiological activity.
Summary of the invention
The technical problem to be solved is to provide a kind of nutrient composition content high, edible safety
Semen Castaneae, Radix Puerariae complex peptides powder.
The technical problem to be solved realizes by the following technical solutions:
A kind of Semen Castaneae, Radix Puerariae complex peptides powder, be prepared from using Semen Castaneae and Radix Puerariae as raw material, wherein Semen Castaneae
It is 1-99:1-99 with the weight proportion of Radix Puerariae；
The preparation method of above-mentioned complex peptides powder is as follows:
(1), after the fresh Semen Castaneae after maturation and fresh Radix Puerariae being cleaned out, it it is 55-65 DEG C in temperature
Dried 40-50 minute in cold air drying equipment, then pulverized 80 mesh sieves, obtained compound full powder；
(2) by the humidity regulation of compound full powder to 40-42%, send in microwave processing equipment, in Microwave Frequency
Being spaced microwave treatment under rate 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10
Minute, it is carried out continuously 3-4 time；
(3) the compound full powder after microwave treatment is taken by weight 1 (compound): 12-18 (water) left and right
Add water, soak 1.5 hours in 75 DEG C of water-baths, be subsequently adding Esterified Enzyme bacterium solution and stir and keep temperature
Extract 1～4 hour at 40～60 DEG C, obtain extracting solution stand-by；
Wherein the addition of Esterified Enzyme bacterium solution is the 0.1-1% of compound full grain weight amount；Esterified Enzyme bacterium solution is applied
Can strengthen the aroma-producing function of Gly-His-Lys in Gly-His-Lys enzyme treatment process, keep product natural flavour mountaineous achieved with
Remarkable result.
(4) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain sucking filtration
After peptide liquid；
(5) the peptide liquid after sucking filtration is carried out vacuum spray drying, collect dried object, pulverize and i.e. obtain complex peptides
Powder.
The preparation method of above-mentioned Esterified Enzyme bacterium solution is as follows:
(1) from aged pit mud, filter out quality pit mud 20g, in 68-69 DEG C of heat treatment 10 minutes, be fabricated to
Esterified Enzyme purifying agaric liquid；
(2) preparation culture medium, and sterilization treatment, culture medium is made up of the component of following mass percent:
Quality pit mud 1.5%, Semen arachidis hypogaeae dregs 0.5%, distiller grains powder 2%, yeast rich in selenium powder 0.2%, koji powder 0.8%, Chinese yellow eel
Fishbone powder 2%, Lumbricus powder 2%, sodium acetate 0.3%, ammonium sulfate 0.02%, magnesium sulfate 0.04%, dehydrated alcohol
2%, magnesium phosphate 0.05%, dipotassium hydrogen phosphate 0.2%, pure water 88.39%；
(3) one-level screening, is inoculated into above-mentioned refined solution with the inoculum concentration of 30% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain primary seed solution；
(4) two grades of screenings, are inoculated into primary seed solution with the inoculum concentration of 22.5% and prepare and after sterilizing
Culture medium, in constant incubator keep 30-40 DEG C cultivate 7 days 8 days, obtain secondary seed solution；
(5) three level screen, is inoculated into secondary seed solution with the inoculum concentration of 16% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain three grades of seed liquor；
(6) the vigorous three grade seed liquor filtered out carries out conventional amplification culture more i.e. can obtain producing using
Esterified Enzyme bacterium solution.
The invention has the beneficial effects as follows: the present invention uses Semen Castaneae to coordinate with Radix Puerariae, the complex peptides powder battalion of goods
Form point high, and rich in trace element, it is possible to promote human metabolism, nourishing the brain and improving intelligence, prevention heart and brain
The generation of angiopathy, contributes to the recovery of the disease such as constipation, chronic enteritis, has the merit of life lengthening
Effect.The peptide liquid using said method to prepare, can fully extract the effective ingredient in raw material, make in raw material
Active substance maximize the retention, be easy to again human body overall absorption, technique is advanced, rationally,
Quality controllable.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and effect and be readily apparent from
Solve, below in conjunction with specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of Semen Castaneae, Radix Puerariae complex peptides powder, be prepared from using Semen Castaneae and Radix Puerariae as raw material, wherein Semen Castaneae
It is 1:1 with the weight proportion of Radix Puerariae；
The preparation method of above-mentioned complex peptides powder is as follows:
(1), after the fresh Semen Castaneae after maturation and fresh Radix Puerariae being cleaned out, it it is 55-65 DEG C in temperature
Dried 40-50 minute in cold air drying equipment, then pulverized 80 mesh sieves, obtained compound full powder；
(2) by the humidity regulation of compound full powder to 40-42%, send in microwave processing equipment, in Microwave Frequency
Being spaced microwave treatment under rate 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10
Minute, it is carried out continuously 3-4 time；
(3) take the compound full powder after microwave treatment to add water, in 75 DEG C of water-baths by weight about 1:12-18
Middle immersion 1.5 hours, is subsequently adding Esterified Enzyme bacterium solution and stirs and maintain the temperature at 40～60 DEG C of extractions
1～4 hour, obtain extracting solution stand-by；
Wherein the addition of Esterified Enzyme bacterium solution is the 0.1-1% of compound full grain weight amount；Esterified Enzyme bacterium solution is applied
Can strengthen the aroma-producing function of Gly-His-Lys in Gly-His-Lys enzyme treatment process, keep product natural flavour mountaineous achieved with
Remarkable result.
(4) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain sucking filtration
After peptide liquid；
(5) the peptide liquid after sucking filtration is carried out vacuum spray drying, collect dried object, pulverize and i.e. obtain complex peptides
Powder.
The preparation method of above-mentioned Esterified Enzyme bacterium solution is as follows:
(1) from aged pit mud, filter out quality pit mud 20g, in 68-69 DEG C of heat treatment 10 minutes, be fabricated to
Esterified Enzyme purifying agaric liquid；
(2) preparation culture medium, and sterilization treatment, culture medium is made up of the component of following mass percent:
Quality pit mud 1.5%, Semen arachidis hypogaeae dregs 0.5%, distiller grains powder 2%, yeast rich in selenium powder 0.2%, koji powder 0.8%, Chinese yellow eel
Fishbone powder 2%, Lumbricus powder 2%, sodium acetate 0.3%, ammonium sulfate 0.02%, magnesium sulfate 0.04%, dehydrated alcohol
2%, magnesium phosphate 0.05%, dipotassium hydrogen phosphate 0.2%, pure water 88.39%；
(3) one-level screening, is inoculated into above-mentioned refined solution with the inoculum concentration of 30% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain primary seed solution；
(4) two grades of screenings, are inoculated into primary seed solution with the inoculum concentration of 22.5% and prepare and after sterilizing
Culture medium, in constant incubator keep 30-40 DEG C cultivate 7 days 8 days, obtain secondary seed solution；
(5) three level screen, is inoculated into secondary seed solution with the inoculum concentration of 16% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain three grades of seed liquor；
(6) the vigorous three grade seed liquor filtered out carries out conventional amplification culture more i.e. can obtain producing using
Esterified Enzyme bacterium solution.
Embodiment 2
A kind of Semen Castaneae, Radix Puerariae complex peptides powder, be prepared from using Semen Castaneae and Radix Puerariae as raw material, wherein Semen Castaneae
It is 5:3 with the weight proportion of Radix Puerariae；
The preparation method of above-mentioned complex peptides powder is as follows:
(1), after the fresh Semen Castaneae after maturation and fresh Radix Puerariae being cleaned out, it it is 55-65 DEG C in temperature
Dried 40-50 minute in cold air drying equipment, then pulverized 80 mesh sieves, obtained compound full powder；
(2) by the humidity regulation of compound full powder to 40-42%, send in microwave processing equipment, in Microwave Frequency
Being spaced microwave treatment under rate 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10
Minute, it is carried out continuously 3-4 time；
(3) take the compound full powder after microwave treatment to add water, in 75 DEG C of water-baths by weight about 1:12-18
Middle immersion 1.5 hours, is subsequently adding Esterified Enzyme bacterium solution and stirs and maintain the temperature at 40～60 DEG C of extractions
1～4 hour, obtain extracting solution stand-by；
Wherein the addition of Esterified Enzyme bacterium solution is the 0.1-1% of compound full grain weight amount；Esterified Enzyme bacterium solution is applied
Can strengthen the aroma-producing function of Gly-His-Lys in Gly-His-Lys enzyme treatment process, keep product natural flavour mountaineous achieved with
Remarkable result.
(4) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain sucking filtration
After peptide liquid；
(5) the peptide liquid after sucking filtration is carried out vacuum spray drying, collect dried object, pulverize and i.e. obtain complex peptides
Powder.
The preparation method of above-mentioned Esterified Enzyme bacterium solution is as follows:
(1) from aged pit mud, filter out quality pit mud 20g, in 68-69 DEG C of heat treatment 10 minutes, be fabricated to
Esterified Enzyme purifying agaric liquid；
(2) preparation culture medium, and sterilization treatment, culture medium is made up of the component of following mass percent:
Quality pit mud 1.5%, Semen arachidis hypogaeae dregs 0.5%, distiller grains powder 2%, yeast rich in selenium powder 0.2%, koji powder 0.8%, Chinese yellow eel
Fishbone powder 2%, Lumbricus powder 2%, sodium acetate 0.3%, ammonium sulfate 0.02%, magnesium sulfate 0.04%, dehydrated alcohol
2%, magnesium phosphate 0.05%, dipotassium hydrogen phosphate 0.2%, pure water 88.39%；
(3) one-level screening, is inoculated into above-mentioned refined solution with the inoculum concentration of 30% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain primary seed solution；
(4) two grades of screenings, are inoculated into primary seed solution with the inoculum concentration of 22.5% and prepare and after sterilizing
Culture medium, in constant incubator keep 30-40 DEG C cultivate 7 days 8 days, obtain secondary seed solution；
(5) three level screen, is inoculated into secondary seed solution with the inoculum concentration of 16% and prepares and after sterilizing
Culture medium, keeps 30-40 DEG C in constant incubator and cultivates 7 days 8 days, obtain three grades of seed liquor；
(6) the vigorous three grade seed liquor filtered out carries out conventional amplification culture more i.e. can obtain producing using
Esterified Enzyme bacterium solution.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.One's own profession
Skilled person will appreciate that of industry, the present invention is not restricted to the described embodiments, above-described embodiment and explanation
The principle that the present invention is simply described described in book, without departing from the spirit and scope of the present invention,
The present invention also has various changes and modifications, and these changes and improvements both fall within claimed invention model
In enclosing.Claimed scope is defined by appending claims and equivalent thereof.
Claims (1)
1. a Semen Castaneae, Radix Puerariae complex peptides powder, it is characterised in that prepare as raw material using Semen Castaneae and Radix Puerariae
Forming, wherein the weight proportion of Semen Castaneae and Radix Puerariae is 1-99:1-99；
The preparation method of above-mentioned complex peptides powder is as follows:
(1), after the fresh Semen Castaneae after maturation and fresh Radix Puerariae being cleaned out, it it is 55-65 DEG C in temperature
Dried 40-50 minute in cold air drying equipment, then pulverized 80 mesh sieves, obtained compound full powder；
(2) by the humidity regulation of compound full powder to 40-42%, send in microwave processing equipment, in Microwave Frequency
Being spaced microwave treatment under rate 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10
Minute, it is carried out continuously 3-4 time；
(3) take the compound full powder after microwave treatment to add water, in 75 DEG C of water-baths by weight about 1:12-18
Middle immersion 1.5 hours, is subsequently adding Esterified Enzyme bacterium solution and stirs and maintain the temperature at 40～60 DEG C of extractions
1～4 hour, obtain extracting solution stand-by；
Wherein the addition of Esterified Enzyme bacterium solution is the 0.1-1% of compound full grain weight amount；
(4) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain sucking filtration
After peptide liquid；
(5) the peptide liquid after sucking filtration is carried out vacuum spray drying, collect dried object, pulverize and i.e. obtain complex peptides
Powder.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101353688A (en) * | 2008-09-27 | 2009-01-28 | 邹远东 | Preparation and use of kudzu root peptides |
| CN103478397A (en) * | 2013-08-16 | 2014-01-01 | 金寨双河源农业技术开发有限公司 | Total chestnut peptide nourishment and preparation method thereof |
| CN105368905A (en) * | 2015-12-09 | 2016-03-02 | 曾志亮 | Microwave-assisted method for preparing pea protein polypeptides |
-
2016
- 2016-04-07 CN CN201610214810.6A patent/CN105831387A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101353688A (en) * | 2008-09-27 | 2009-01-28 | 邹远东 | Preparation and use of kudzu root peptides |
| CN103478397A (en) * | 2013-08-16 | 2014-01-01 | 金寨双河源农业技术开发有限公司 | Total chestnut peptide nourishment and preparation method thereof |
| CN105368905A (en) * | 2015-12-09 | 2016-03-02 | 曾志亮 | Microwave-assisted method for preparing pea protein polypeptides |
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