CN105823753A - Detection method for content of manganese of jujube crown layer - Google Patents
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- 238000001514 detection method Methods 0.000 title claims abstract description 28
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 title abstract description 7
- 229910052748 manganese Inorganic materials 0.000 title abstract description 7
- 239000011572 manganese Substances 0.000 title abstract description 7
- 240000008866 Ziziphus nummularia Species 0.000 title 1
- 238000002835 absorbance Methods 0.000 claims abstract description 13
- 238000012937 correction Methods 0.000 claims abstract description 4
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 4
- 244000126002 Ziziphus vulgaris Species 0.000 claims description 39
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims description 39
- 238000001035 drying Methods 0.000 claims description 7
- 238000000227 grinding Methods 0.000 claims description 6
- 238000009499 grossing Methods 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000000151 deposition Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000000428 dust Substances 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims description 3
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- 229910052573 porcelain Inorganic materials 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 241001247821 Ziziphus Species 0.000 abstract description 4
- 238000004497 NIR spectroscopy Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical compound CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Biochemistry (AREA)
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Abstract
The invention discloses a detection method for the content of manganese of a jujube crown layer .The detection method includes the following steps that leaves of the to-be-detected jujube crown layer are collected according to the representativeness, put into an oven, fixed and dried to be ground and screened by a 100-mesh sieve; the spectroscopic data of the jujube-leaf ground samples is collected through a near-infrared spectroscopy; the spectroscopic data is subjected to baseline correction to be smoothed; the absorbance of the samples is extracted respectively; the absorbance of all the samples is substituted into a detection model, and the content of the manganese of the jujube crown layer is calculated and obtained .The detection method has the advantages of being free of pollution, easy and convenient to operate, rapid and accurate, and is suitable for application and popularization.
Description
Technical field
The invention belongs to plant cultivation field of engineering technology, relate to the detection method of a kind of Fe content, specifically, relate to the detection method of a kind of jujube tree canopy Fe content.
Background technology
Manganese is the requisite nutrient of jujube growth, and quick obtaining manganese information is monitoring growth and development of jujube trees and the premise of quality guarantee.In prior art, the detection method of jujube tree canopy calcium content needs to prepare chemical reagent, and during mensuration, chemistry tail liquid emissions damages to environment with to human body, and there is complex operation step, the defect of detection time length.It is badly in need of the detection method of pollution-free, easy and simple to handle, the most accurate a kind of jujube tree canopy Fe content at present.
Summary of the invention
In order to overcome defect present in prior art, the present invention proposes a kind of pollution-free, easy and simple to handle, the detection method of the most accurate jujube tree canopy Fe content, the method achieve the quick and precisely detection of jujube tree canopy Fe content, need not prepare any chemical reagent, avoid the chemistry tail liquid emissions pollution to environment and the injury to human body during mensuration, also greatly simplify operating procedure simultaneously, shorten the detection time, the demand requiring to obtain canopy Fe content data in agricultural production in the large area Fructus Jujubae garden short time can be met, for in jujube tree field management manganese precisely use the means of quick obtaining dose foundation of providing.
Its technical scheme is as follows:
The detection method of a kind of jujube tree canopy Fe content, comprises the following steps:
(1) gather the blade of Fe content jujube tree canopy to be determined, be loaded in the paper envelopes of numbering.
(2), after being rinsed sample, complete drying.
(3) grinding is sieved, and is loaded in sample bag.
(4) sample absorbance is measured with near infrared spectrometer.
(5) spectroscopic data is carried out baseline correction and smoothing processing.
null(6) each sample is extracted in wave number 4130.775、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance.
(7) absorbance of each wave number is substituted into jujube tree canopy Fe content detection model:
Y=-0.0506+0.6966982x1-51.53961x2+5.067605x3+30.03444x4+32.61036x5+9.048948x6+20.93617x7+22.49618x8-15.85952x9-2.812272x10-2.336486x11-15.28067x12-11.5837x13-10.21183x14-16.17481x15-18.89252x16-21.76718x17-4.380602x18+3.0373x19-1.631123x20+20.24516x21+23.09162x22+5.86936x23-1.412536x24+13.94756x25-10.13427x26+3.814411x27-4.677543x28+8.645446x29+8.624619x30-0.2701266x31-7.749272x32-10.77078x33-6.269518x34+3.088822x35+2.387544x36+23.77513x37+21.62175x38-3.452066x39-7.632906x40-10.21808x41-29.61866x42+14.43646x43+20.04975x4 4-11.31772x45-17.52046x46
Wherein, y is jujube tree canopy Fe content, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、x14、x15、x16、x17、x18、x19、x20、x21、x22、x23、x24、x25、x26、x27、x28、x29、x30、x31、x32、x33、x34、x35、x36、x37、x38、x39、x40、x41、x42、x43、x44、x45、x46nullIt is respectively jujube tree canopy sample in wave number 4130.775、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance.
Preferably, the blade gathering Fe content jujube tree canopy to be determined described in step (1), the Chinese Jujube gathered must be representative, need to gather each 10 of position, canopy upper, middle and lower blade during collection, and each canopy gathers 30 blades.
Preferably, sample is rinsed described in step (2), need first rinse out after the depositing dust that blade is detained again with distilled water flushing 1～2 times with tap water.
Preferably, the drying that completes described in step (2), particularly as follows: first complete 30 minutes at 105 degrees Celsius, is dried to constant weight under 80 degrees celsius.
Preferably, the ground sieve described in step (3) is particularly as follows: be ground with porcelain mortar immediately after sample drying is the coldest, and the degree of grinding is that all samples all can pass through 100 mesh sieves.
Preferably, the sample bag described in step (3) is required to be pulling mouth and seals bag, in case the sample moisture absorption.
Preferably, the smoothing processing described in step (5) is specially Savitzky-Golay, and smooth window is 5.
The invention have the benefit that
Present invention achieves the quick and precisely detection of jujube tree canopy Fe content, need not prepare any chemical reagent, avoid the chemistry tail liquid emissions pollution to environment and the injury to human body during mensuration, also greatly simplify operating procedure simultaneously, shorten the detection time, require to obtain the demand of canopy Fe content data in meeting large area Fructus Jujubae garden short time in agricultural production, in jujube tree field management manganese precisely use the means of quick obtaining dose foundation of providing.The method has feature pollution-free, easy and simple to handle, the most accurate, is suitable for popularization and application.
Accompanying drawing explanation
Fig. 1 is jujube tree canopy Fe content measured value and detected value degree of fitting.
Detailed description of the invention
With detailed description of the invention, technical scheme is described in more detail below in conjunction with the accompanying drawings.
The detection method of a kind of jujube tree canopy Fe content, comprises the following steps:
(1) gathering Fe content jujube tree canopy leaves to be determined, each canopy gathers each-10 of the blade of 3 levels in upper, middle and lower, and each canopy gathers 30 Chinese Jujubes altogether, and the blade being collected is put in the paper envelopes bag of numbering.This example acquires 90 canopy samples in difference Tuan Changzao garden, Alar City, Xinjiang altogether.
(2) the jujube tree canopy sample that is collected first is rinsed with tap water with laboratory, after the depositing dust of the residual cleaning blade surface, then with distilled water flushing 1-2 time, be loaded in the envelope of reference numeral.
(3) envelope being put in baking oven, dry 30 minutes prior to 105 degrees Celsius, drying to constant weight temperature being adjusted to 80 degrees Celsius.
(4) after the sample dried is cooled to room temperature, grinding Folium Jujubae with porcelain mortar, after grinding, complete 100 mesh sieves of whole mistakes, install with pulling mouth plastic sealing bag, in case the sample moisture absorption.
(5) ground sample is put in the transparent culture dish build-in test near-infrared data of diameter 5cm, and the near infrared spectrometer of this example is AntarisIIFT-NIR Fourier transformation type spectrogrph, spectral range: 4000～10000cm-1, spectral resolution: 4cm-1, scanning times: 32 times, the meansigma methods of 32 times is the absorbance that this sample is final.
(6) after spectral data being carried out baseline correction, smoothing by Savitzky-Golay method, smooth window is 5.
null(7) each sample 4130.775 is extracted、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance, substitute into detection model:
Y=-0.0506+0.6966982x1-51.53961x2+5.067605x3+30.03444x4+32.61036x5+9.048948x6+20.93617x7+22.49618x8-15.85952x9-2.812272x10-2.336486x11-15.28067x12-11.5837x13-10.21183x14-16.17481x15-18.89252x16-21.76718x17-4.380602x18+3.0373x19-1.631123x20+20.24516x21+23.09162x22+5.86936x23-1.412536x24+13.94756x25-10.13427x26+3.814411x27-4.677543x28+8.645446x29+8.624619x30-0.2701266x31-7.749272x32-10.77078x33-6.269518x34+3.088822x35+2.387544x36+23.77513x37+21.62175x38-3.452066x39-7.632906x40-10.21808x41-29.61866x42+14.43646x43+20.04975x4 4-11.31772x45-17.52046x46
Wherein, y is jujube tree canopy Fe content, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、x14、x15、x16、x17、x18、x19、x20、x21、x22、x23、x24、x25、x26、x27、x28、x29、x30、x31、x32、x33、x34、x35、x36、x37、x38、x39、x40、x41、x42、x43、x44、x45、x46nullIt is respectively jujube tree canopy sample in wave number 4130.775、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance.
Table 1 and Fig. 1 shows the degree of fitting in this example between actual value and detected value.Therebetween meansigma methods, maximum and minima difference is little, the coefficient of determination (R2) reach 0.77, root-mean-square error (RMSE) is only 0.00478mg/g, average relative error (MRE) only 5.695%, and the ratio (RPD) of standard deviation and root-mean-square error up to 2.097, according to evaluation criterion, RPD ＞ 2.0 illustrates that model can the attribute of high-precision estimation sample.Thus illustrating, the method for the present invention can the most quickly measure the Fe content of jujube tree canopy.
Table 1 jujube tree canopy Fe content actual value is added up with detected value
The above; it is only the present invention preferably detailed description of the invention; protection scope of the present invention is not limited to this; any those familiar with the art is in the technical scope of present disclosure, and the simple change of the technical scheme that can become apparent to or equivalence are replaced and each fallen within protection scope of the present invention.
Claims (7)
1. the detection method of a jujube tree canopy Fe content, it is characterised in that comprise the following steps:
(1) gather the blade of Fe content jujube tree canopy to be determined, be loaded in the paper envelopes of numbering；
(2), after being rinsed sample, complete drying；
(3) grinding is sieved, and is loaded in sample bag；
(4) sample absorbance is measured with near infrared spectrometer；
(5) spectroscopic data is carried out baseline correction and smoothing processing；
null(6) each sample is extracted in wave number 4130.775、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance；
(7) absorbance of each wave number is substituted into jujube tree canopy Fe content detection model:
Y=-0.0506+0.6966982x1-51.53961x2+5.067605x3+30.03444x4+32.61036x5+9.048948x6+20.93617x7+22.49618x8-15.85952x9-2.812272x10-2.336486x11-15.28067x12-11.5837x13-10.21183x14-16.17481x15-18.89252x16-21.76718x17-4.380602x18+3.0373x19-1.631123x20+20.24516x21+23.09162x22+5.86936x23-1.412536x24+13.94756x25-10.13427x26+3.814411x27-4.677543x28+8.645446x29+8.624619x30-0.2701266x31-7.749272x32-10.77078x33-6.269518x34+3.088822x35+2.387544x36+23.77513x37+21.62175x38-3.452066x39-7.632906x40-10.21808x41-29.61866x42+14.43646x43+20.04975x4 4-11.31772x45-17.52046x46
Wherein, y is jujube tree canopy Fe content, and unit is mg/g, x1、x2、x3、x4、x5、x6、x7、x8、x9、x10、x11、x12、x13、x14、x15、x16、x17、x18、x19、x20、x21、x22、x23、x24、x25、x26、x27、x28、x29、x30、x31、x32、x33、x34、x35、x36、x37、x38、x39、x40、x41、x42、x43、x44、x45、x46nullIt is respectively jujube tree canopy sample in wave number 4130.775、6749.633、6792.06、6795.917、6799.773、6803.63、6819.058、6822.915、6857.627、6861.484、6865.341、6869.198、6873.055、6876.912、6880.769、6884.626、6888.483、6923.195、6927.052、6930.909、6934.766、6938.623、6942.48、6946.337、6950.194、6957.908、6973.335、6977.192、6981.049、6984.906、6988.763、6992.62、6996.477、7000.334、7004.191、7308.889、7440.024、7443.881、7497.878、7501.735、7505.592、7517.163、9129.361、9133.218、9387.775、9391.633cm-1Absorbance.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterized in that, the blade gathering Fe content jujube tree canopy to be determined described in step (1), the Chinese Jujube gathered must be representative, need to gather each 10 of position, canopy upper, middle and lower blade during collection, each canopy gathers 30 blades.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterised in that being rinsed sample described in step (2), needs first rinse out after the depositing dust that blade is detained again with distilled water flushing 1～2 times with tap water.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterised in that the drying that completes described in step (2), particularly as follows: first complete 30 minutes at 105 degrees Celsius, is dried to constant weight under 80 degrees celsius.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterized in that, ground sieve described in step (3) is particularly as follows: be ground with porcelain mortar immediately after sample drying is the coldest, and the degree of grinding is that all samples all can pass through 100 mesh sieves.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterised in that the sample bag described in step (3) is required to be pulling mouth and seals bag, in case the sample moisture absorption.
The detection method of jujube tree canopy Fe content the most according to claim 1, it is characterised in that the smoothing processing described in step (5) is specially Savitzky-Golay, smooth window is 5.
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|---|---|---|---|---|
| CN106248627A (en) * | 2016-10-09 | 2016-12-21 | 塔里木大学 | A kind of detection method of the humus content of the soil |
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