CN105823741B - The detection method of butyraldehyde content in a kind of air - Google Patents
The detection method of butyraldehyde content in a kind of air Download PDFInfo
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Abstract
The invention belongs to air detection technical field, and in particular to the detection method of butyraldehyde content in a kind of air.The detection method is realized especially by following steps：Centrifugal treating spectrophotometer detects after sampling the solvent analysis freezing.The determining instrument spectrophotometer popularity rate that the present invention uses is high, cheap, effectively reduces testing cost；Detection process is relatively easy, easy to operate；The present invention carries out parsing joint deepfreeze using double solvents, farthest improves Detection accuracy, reduces testing cost.
Description
Technical field
The invention belongs to air detection technical field, and in particular to the detection method of butyraldehyde content in a kind of air.
Background technology
Butyraldehyde is moderate noxious material, and butyraldehyde has that asphyxiating aldehyde is smelly in air, has to stimulate to eye, respiratory mucosa and makees
With.The short-time contact safe level of butyraldehyde is 10mg/m in workplace air3.Butyraldehyde content is to protecting in accurate detection air
It is significant to protect operating personnel's health.The industries such as petrochemical industry, rubber, fire coal can produce butyraldehyde, motor-vehicle tail-gas,
Also contain butyraldehyde in cooking fume, smoke from cigarette.
At present, the method for measuring butyraldehyde in air mainly has gas chromatography and liquid chromatography.Wherein gas chromatography
Gathered using thermal desorption type silicone tube, sample introduction after thermal desorption, after chromatography post separation, flame ionization ditector detection；Liquid
Phase chromatography is gathered using 2,4-dinitrophenylhydrazine silicone tube, is eluted with acetonitrile ultrasound, after high performance liquid chromatography separation, two poles
The shortcomings that pipe array detector detects, the prior art is as follows：（1）Gas chromatography need to configure pyrolysis haustorium at the same time, which exists
The frequency of use in laboratory is not high, causes testing cost to raise；Calibrating gas need to carry out secondary dilution before use, and it is accurate to exist
Spend the risk reduced；If the butyraldehyde concentration in stripping gas exceedes measurement range, measured again after need to being diluted with clean air, meeting
Cause measurement result relatively low.（2）The 2,4-dinitrophenylhydrazine silicone tube preparation process used in liquid chromatography is cumbersome, collection effect
Rate is not high；Frequency of use of the diode array detector detection in laboratory be not high, causes testing cost to raise.Since butyraldehyde should
For spectrophotometer method, during preparing detection liquid, formaldehyde in sample etc. understands interference detection results and fourth to a certain extent
Aldehyde cannot parse completely, cause accuracy rate of testing result to reduce.
The content of the invention
The present invention is to overcome the shortcomings of existing analysis method, there is provided a kind of solvent-borne type silicone tube gathers, mixed solvent desorbs,
The detection method of butyraldehyde content in Air by Spectrophotometry, its detection process is simple, used instrument and equipment popularity rate
Height, measurement result is accurately and reliably.
The technical scheme is that：
The present invention provides a kind of detection method of butyraldehyde content in air, comprise the following steps：
（1）In sampled point, solvent-borne type silicone tube both ends collection air sample is opened；
（2）Silica gel in sample and blank silicone tube is respectively put into solvent desorption bottle, it is each to add 5.00mL ethanol-the third
Alcohol mixed solvent, after closing, shakes 1min, desorbs 30min, obtain stripping liquid；
（3）Stripping liquid is placed in -15 DEG C of freezing 30min, centrifugal treating is carried out after taking-up immediately, takes supernatant；
（4）1.00mL supernatants are taken in colorimetric cylinder, add 1.0mL2,4- dinitrophenylhydrazines alcohol saturated solution and
0.05mL hydrochloric acid, Gai Sai, places 30min, cooling, adds the potassium hydroxide-ethanol solution of 5.0mL100g/L, mix in 50 DEG C of water-baths
It is even, after placing 5min, in measuring absorbance on spectrophotometer.
Further, the solvent-borne type silicone tube is the flow collection air sample with 500mL/min, and acquisition time is
30min。
The volume of ethanol and propyl alcohol in used mixed solvent-ethanol-propanol solvent mixture when the present invention is parsed
Than for 92:8.
Further, step（4）In, on spectrophotometer at 480nm wavelength measure absorbance.
Parsed, done in the prior art, when being parsed using single alcohol molten using mixed solvent in the present invention
Agent, since single alcohol can not produce interaction as the surface of surfactant molecule and silica gel, it is impossible to reach complete parsing,
So as to influence testing result.The present invention is carried out in use, work of the propyl alcohol except playing solvent using ethanol-propanol solvent mixture
With what is more important can be covered in sorbing material surface as surfactant molecule, at utmost be parsed.This hair
It is bright it is creative desorbed solution is freezed at low temperature in advance, formaldehyde easily forms precipitation in low temperature, after centrifugal treating,
Content of formaldehyde in desorbed solution, joint mixed solvent synergistic effect, so as to improve the accuracy of detection can be reduced.In addition, this Shen
Inventor please has found by many experiments, changes the volume content of ethanol-propyl alcohol, and the accuracy of its testing result has been dropped
It is low.
Advantages of the present invention is：
（1）Determining instrument spectrophotometer popularity rate that this method uses is high, cheap, effectively reduce detection into
This；Detection process is relatively easy, easy to operate；
（2）The present invention carries out parsing joint deepfreeze using double solvents, farthest improves Detection accuracy.
Embodiment
Embodiment 1
The detection method of butyraldehyde content, step are as follows in a kind of air：
（1）In sampled point, silicone tube both ends are opened with the flow collection 30min air samples of 500mL/min, by another silicon
Sebific duct is brought to sampled point, opens silicone tube both ends, is not connected to sampler and is placed 30min；
（2）Silica gel in sample and blank silicone tube is respectively put into solvent desorption bottle, it is each to add 5.00mL ethanol-the third
Alcohol mixed solvent, after closing, shakes 1min, desorbs 30min, obtain stripping liquid；Stripping liquid is placed in -15 DEG C of freezing 30min, is taken out
Carry out centrifugal treating immediately afterwards, take supernatant；
The volume ratio of the ethanol and propyl alcohol is 92:8；
（3）Standard solution is prepared：0.0500g butyraldehyde is weighed in 100mL volumetric flasks, scale is settled to ethanol, shakes
It is even, this solution 2.00mL is pipetted in 100mL volumetric flasks, scale is settled to ethanol, shakes up, and butyraldehyde concentration is 10 in this solution
μg/mL；
（4）The drafting of standard curve：0.00mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL, 1.00mL fourth are pipetted respectively
Aldehyde standard solution adds absorbing liquid to 2.0mL in 6 color-comparison tubes, be made into 0.0 μ g/mL, 2.0 μ g/mL, 4.0 μ g/mL,
6.0 μ g/mL, 8.0 μ g/mL, 10.0 μ g/mL butyraldehyde series.Added into each standard series pipe 1.0mL2,4- dinitrophenylhydrazines-
Alcohol saturated solution and 1 drop hydrochloric acid, Gai Sai, 30min are placed in 50 DEG C of water-baths, cooling, add the potassium hydroxide of 5.0mL100g/L-
Ethanol solution, mix, place 5min after, on spectrophotometer 480nm at measure absorbance.With the absorbance that measures to fourth
Aldehyde concentration（μg/mL）Standard curve is drawn, equation of linear regression is A=0.0101c+0.0118, coefficient R2=0.9997；
（5）Sample measures：1.00mL supernatants are taken in colorimetric cylinder, add 1.0mL2,4- dinitrophenylhydrazine ethanol saturations
Solution and 0.05mL hydrochloric acid, Gai Sai, places 30min, cooling, adds the ethanolic potassium hydroxide of 5.0mL100g/L molten in 50 DEG C of water-baths
Liquid, mixes, after placing 5min, in measuring absorbance on spectrophotometer；The absorbance for measuring sample solution is 0.103, blank
The absorbance of sample solution is 0.000.
（6）It is 10.0 μ g/mL to look into standard curve and obtain butyraldehyde concentration in sample solution, butyraldehyde concentration in blank sample solution
For 0.0 μ g/mL；
（7）It is 1.7mg/m that the butyraldehyde concentration in sampled point air is calculated by formula3；
Specific formula for calculation is：
In formula：The concentration values of butyraldehyde in C-air, unit are every cubic metre of milligram（mg/m3）；
The concentration values of butyraldehyde, unit are micrograms per millilitre in c-determination sample absorbing liquid（μg/mL）；
c0The concentration values of butyraldehyde in-measure blank sample absorbing liquid, unit is micrograms per millilitre（μg/mL）；
2.5-sample extension rate；
The standard sample volume of V-sample.
Comparative example 1
The detection method of butyraldehyde content in a kind of air, specific steps are essentially identical with embodiment 1, and difference is：
When sample parses, using only ethanol single solvent, other steps are the same as embodiment 1.
Comparative example 2
The detection method of butyraldehyde content in a kind of air, specific steps are essentially identical with embodiment 1, and difference is：
Using single etoh solvent during parsing, at the same omit " by stripping liquid be placed in -15 DEG C freezing 30min, carried out immediately after taking-up from
Heart processing, takes supernatant " step processing, other steps are the same as embodiment 1.
Effect test
1st, the butyraldehyde standard solution of 15 μ L is accurately added into blank silicone tube（Concentration is 500 μ g/mL）, it is utilized respectively reality
The detection of example 1 and the method progress butyraldehyde content of comparative example 1-2 offers is applied, concrete outcome is shown in Table 1.
Table 1
2nd, the butyraldehyde standard solution of 15 μ L is accurately added into blank silicone tube（Concentration is 500 μ g/mL）, then add few
Perhaps formaldehyde standard solution, is utilized respectively the detection that the method that embodiment 1 and comparative example 2 provide carries out butyraldehyde content, concrete outcome
It is shown in Table 2.
Table 2
3rd, recovery test（Method accuracy test）：It is accurate respectively into blank silicone tube to add different volumes butyraldehyde mark
Quasi- solution（Concentration is 500 μ g/mL）, its rate of recovery is measured using the method for the offer of embodiment 1.Determination of recovery rates the results are shown in Table/
3。
Table 3
4th, standard deviation（Method precision）Experiment:11 blank silicone tubes are taken, add 20 μ L butyraldehyde standards thereto
Solution（Concentration is 500 μ g/mL）, the method provided by embodiment 1 is measured, according to the absorbance measured and linear regression side
Journey, calculates butyraldehyde concentration, and result of calculation is shown in Table 4.
4 precision measurement result of table（n=11）
Above-mentioned specific embodiment is only that spirit of the present invention is illustrated.The technology people of the technical field of the invention
Member can make described specific embodiment various modifications, supplement or with substituting in a similar way.But not
The spirit or beyond the scope of the appended claims of the present invention can be deviateed.
Claims (4)
1. the detection method of butyraldehyde content in a kind of air, it is characterised in that comprise the following steps：
（1）In sampled point, solvent-borne type silicone tube both ends collection air sample is opened；
（2）Silica gel in sample and blank silicone tube is respectively put into solvent desorption bottle, each 5.00mL ethanol-propyl alcohol that adds mixes
Bonding solvent, after closing, shakes 1min, desorbs 30min, obtain stripping liquid；
（3）Stripping liquid is placed in -15 DEG C of freezing 30min, centrifugal treating is carried out after taking-up immediately, takes supernatant；
（4）1.00mL supernatants are taken in colorimetric cylinder, add 1.0mL2,4- dinitrophenylhydrazines alcohol saturated solution and 0.05mL salt
Acid, Gai Sai, places 30min, cooling, adds the potassium hydroxide-ethanol solution of 5.0mL100g/L, mix, place in 50 DEG C of water-baths
After 5min, in measuring absorbance on spectrophotometer；
（5）Configuration standard solution：0.0500g butyraldehyde is weighed in 100mL volumetric flasks, scale is settled to ethanol, shakes up, pipette
This solution 2.00mL is settled to scale in 100mL volumetric flasks, with ethanol, shakes up, and butyraldehyde concentration is 10 μ g/mL in this solution；
0.00mL, 0.20mL, 0.40mL, 0.60mL, 0.80mL, 1.00mL butyraldehyde standard solution are pipetted respectively in 6 color-comparison tubes
In, add absorbing liquid to be made into 0.0 μ g/mL, 2.0 μ g/mL, 4.0 μ g/mL, 6.0 μ g/mL, 8.0 μ g/mL, 10.0 μ g/mL to 2.0mL
Butyraldehyde series；1.0mL2 is added into each standard series pipe, drip hydrochloric acid for 4- dinitrophenylhydrazines-alcohol saturated solution and 1, Gai Sai,
30min is placed in 50 DEG C of water-baths, cooling, adds the potassium hydroxide-ethanol solution of 5.0mL100g/L, mixes, after placing 5min, in
Absorbance is measured on spectrophotometer at 480nm；With the absorbance that measures to butyraldehyde concentration（μg/mL）Draw standard curve,
Equation of linear regression is A=0.0101c+0.0118, coefficient R2=0.9997；
（6）Look into standard curve and obtain butyraldehyde concentration in sample solution, butyraldehyde concentration in blank sample solution；Calculate and adopt by formula
Butyraldehyde concentration in sampling point air；
The formula is：
In formula：The concentration values of butyraldehyde in C-air, unit are every cubic metre of milligram；Butyraldehyde in c-determination sample absorbing liquid
Concentration values, unit are micrograms per millilitre；c0The concentration values of butyraldehyde, unit are every for microgram in-measure blank sample absorbing liquid
Milliliter；2.5-sample extension rate；The standard sample volume of V-sample.
2. detection method according to claim 1, it is characterised in that the solvent-borne type silicone tube is with 500mL/min
Flow collection air sample, acquisition time 30min.
3. detection method according to claim 1, it is characterised in that ethanol and third in the ethanol-propanol solvent mixture
The volume ratio of alcohol is 92:8.
4. detection method according to claim 1, it is characterised in that step（4）In, in 480nm ripples on spectrophotometer
Strong point measures absorbance.
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| CN112129748A (en) * | 2019-06-25 | 2020-12-25 | 内蒙古伊泰煤基新材料研究院有限公司 | Method suitable for determining carbonyl compounds in FT synthetic oil |
| CN110794061A (en) * | 2019-11-14 | 2020-02-14 | 苏州新世纪检测技术服务有限公司 | Butyraldehyde automatic thermal analysis gas chromatography detection method |
| CN111257264B (en) * | 2020-03-18 | 2023-02-28 | 上海纽脉医疗科技有限公司 | Method for detecting aldehyde group content in biological tissue |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19800537A1 (en) * | 1998-01-09 | 1998-10-08 | Andrea Bueldt | N-Methyl-4-hydrazino-7-nitro-benzo-2-oxa-1,3-diazole |
| JPH1164218A (en) * | 1997-08-26 | 1999-03-05 | Matsushita Electric Works Ltd | Method for quantifying concentration of body fluid component |
| TW200408803A (en) * | 2002-11-18 | 2004-06-01 | Jiun-Huei Li | Method for detecting volatile aldehyde |
| CN102565262A (en) * | 2011-12-23 | 2012-07-11 | 江苏中烟工业有限责任公司 | Determination method for hydrogen sulfide in mainstream smoke of cigarette |
| KR20120129387A (en) * | 2011-05-20 | 2012-11-28 | 주식회사 씨맥 | Potassium ion concentration measuring method and the measuring kit using thereof |
| CN102818864A (en) * | 2012-06-20 | 2012-12-12 | 上海博物馆 | Passive sampling detection method of formaldehyde in environment |
| CN103257137A (en) * | 2012-02-17 | 2013-08-21 | 谱尼测试科技股份有限公司 | Method of determining content of methyl ethyl ketone peroxide in workplace air through UV spectrophotometer |
| CN104569245A (en) * | 2014-12-08 | 2015-04-29 | 江苏泰洁检测技术有限公司 | Method for measuring concentration of cyclohexylamine in aliphatic amines of workplace |
-
2016
- 2016-03-11 CN CN201610137888.2A patent/CN105823741B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1164218A (en) * | 1997-08-26 | 1999-03-05 | Matsushita Electric Works Ltd | Method for quantifying concentration of body fluid component |
| DE19800537A1 (en) * | 1998-01-09 | 1998-10-08 | Andrea Bueldt | N-Methyl-4-hydrazino-7-nitro-benzo-2-oxa-1,3-diazole |
| TW200408803A (en) * | 2002-11-18 | 2004-06-01 | Jiun-Huei Li | Method for detecting volatile aldehyde |
| KR20120129387A (en) * | 2011-05-20 | 2012-11-28 | 주식회사 씨맥 | Potassium ion concentration measuring method and the measuring kit using thereof |
| CN102565262A (en) * | 2011-12-23 | 2012-07-11 | 江苏中烟工业有限责任公司 | Determination method for hydrogen sulfide in mainstream smoke of cigarette |
| CN103257137A (en) * | 2012-02-17 | 2013-08-21 | 谱尼测试科技股份有限公司 | Method of determining content of methyl ethyl ketone peroxide in workplace air through UV spectrophotometer |
| CN102818864A (en) * | 2012-06-20 | 2012-12-12 | 上海博物馆 | Passive sampling detection method of formaldehyde in environment |
| CN104569245A (en) * | 2014-12-08 | 2015-04-29 | 江苏泰洁检测技术有限公司 | Method for measuring concentration of cyclohexylamine in aliphatic amines of workplace |
Non-Patent Citations (1)
| Title |
|---|
| Simultaneous determination of airborne acetaldehyde, acetone,2-butanone, and cyclohexanone using sampling tubes with 2,4-dinitrophenylhydrazine-coated solid sorbent;Norbert Binding 等;《Toxicology Letters》;19981231;第96卷;全文 * |
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