CN105803002A - Method for preparing hydrogen by virtue of xylose - Google Patents
Method for preparing hydrogen by virtue of xylose Download PDFInfo
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- CN105803002A CN105803002A CN201610320613.2A CN201610320613A CN105803002A CN 105803002 A CN105803002 A CN 105803002A CN 201610320613 A CN201610320613 A CN 201610320613A CN 105803002 A CN105803002 A CN 105803002A
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Abstract
The invention discloses a method for preparing hydrogen by virtue of xylose. The method comprises the following steps: 1) preparing a klebsiella oxytoca GS-4-08 strain and cultivating so as to obtain a seed solution; 2) inoculating the seed solution into a sterilized hydrogen producing culture medium, and then conducting aerobic growth at 35 DEG C, so that a great amount of klebsiella oxytoca GS-4-08 strain culture solutions are cultivated; 3) conducting nitrogen exposing treatment on the culture solutions, and after the treatment, adding the culture solution, which is cultivated to a last exponential growth stage through the aerobic growth, to a xylose solution according to an inoculation rate of 100%; and 4) conducting an anaerobic reaction and collecting hydrogen. The method disclosed by the invention, which is used for producing the hydrogen through anaerobic fermentation of microorganisms, has the advantages of being able to controlling pollution and protecting environment, capable of achieving a high production capacity and the like, and the method is broad in development prospect. Abundant and cheap renewable lignocellulosic biomasses exist in nature; monosaccharide, as a main hydrolysis ingredient of lignocellulose, contains about 30-40% of five-carbon sugars; therefore, the effective utilization of the hydrogen producing property of the xylose has an important significance for expanding a substrate scope, reducing the cost of biological hydrogen production and achieving the industrialization of the biological hydrogen production.
Description
Technical field
The invention belongs to new energy field, relate to a kind of method utilizing xylose to prepare hydrogen.
Background technology
Hydrogen, as efficient, cleaning, the reproducible energy, is increasingly paid close attention to by people.In multiple hydrogen production process, utilizing microbiological anaerobic fermentation of biomass (such as organic wastewater, organic waste etc.) hydrogen manufacturing, possess the multiple superioritys such as pollution treatment, environmental protection and production capacity, DEVELOPMENT PROSPECT is wide.Researchers have done a lot of fruitful research work, and one of them expands substrate utilization scope exactly.In nature, a large amount of reproducible wood fiber biomass are abundant and cheap, if it is possible to solve to utilize lignocellulose finally to obtain this key issue of hydrogen as raw material, and it will make to obtain cheap hydrogen and realize industrialization to be possibly realized.Lignocellulose is mainly made up of lignin, cellulose and hemicellulose, the hydrolysing component of cellulose and hemicellulose is mainly monosaccharide and a small amount of simple polysaccharide, the hexose that wherein monosaccharide is mainly based on glucose accounts for 60% ~ 70%, pentose based on xylose accounts for 30% ~ 40%, and prepare hydrogen previously with respect to glucose and reported, xylose is not then applied because of problems such as difficult decomposition.Therefore the pentose hydrogen-producing characteristic such as research and utilization xylose, for extension substrate spectrum, reduces biological hydrogen production cost, it is achieved the industrialization of biological hydrogen production has great importance.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, it is an object of the invention to provide a kind of method utilizing xylose to prepare hydrogen, reduces biological hydrogen production cost, it is achieved the industrialization of biological hydrogen production.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is:
A kind of method utilizing xylose to prepare hydrogen, comprises the following steps:
1) takeKlebsiella oxytocaGS-4-08 strain, cultivates and obtains seed liquor；
2) take in the bacterial growth media after seed liquor is inoculated in sterilizing, at 35 DEG C, then carry out aerobic growth；It is substantial amounts of that cultivation obtainsKlebsiella oxytocaGS-4-08 bacteria culture fluid；
3) carry out exposing nitrogen treatment to product hydrogen culture medium, after exposing nitrogen, be added to produce in hydrogen culture medium according to the rate of vaccination of 100% by culture fluid during aerobic growth to exponential growth latter stage；
4) anaerobic reaction, collects hydrogen.
In step 1), cultivate seed liquor with growth medium.
Step 2) in, the formula producing hydrogen culture medium is: 0.5g/L KH2PO4、1.57g/L Na2HPO4·12H2O、0.1g/L MgSO4·7H2O。
Step 2) in, the pH value producing hydrogen culture medium is about 7.0.
Step 2) in, at 35 DEG C, carry out aerobic growth 12h.
In step 3), xylose solution concentration is 2 ~ 8g/L.
In step 4), more than anaerobic reaction 48h.
Beneficial effect: in nature, a large amount of reproducible wood fiber biomass are abundant and cheap, lignocellulose hydrolysing component is mainly monosaccharide, pentose in monosaccharide accounts for 30 ~ 40%, therefore, effectively utilize xylose hydrogen-producing characteristic, for extension substrate spectrum, reduce biological hydrogen production cost, it is achieved the industrialization of biological hydrogen production has great importance.The present invention utilizesKlebsiella oxytocaGS-4-08 strain anaerobic fermentation xylose hydrogen manufacturing, conversion ratio is 38.5mL hydrogen/g xylose, possesses the multiple superioritys such as pollution treatment, environmental protection and production capacity, and DEVELOPMENT PROSPECT is wide, can realize industrialization.
Accompanying drawing explanation
Fig. 1 is the product hydrogen result figure under different xylose concentration；
Fig. 2 is the total hydrogen output result figure under the rate of vaccination of 1%.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
A kind of method utilizing xylose to prepare hydrogen, comprises the following steps:
1) takeKlebsiella oxytocaGS-4-08 strain (strain disclosed in CN105524944A), activates with growth medium, it is thus achieved that seed liquor；The formula of growth medium is: yeast extract 5g/L, peptone 10g/L, sodium chloride 10g/L.
2) take seed liquor, be inoculated in the growth medium after sterilizing, antibacterial activation temperature 35 DEG C, rotating speed 180r/min, aerobic growth 12 hours；Carry out again exposing nitrogen treatment；The formula producing hydrogen culture medium (fermentation medium) is: KH2PO4
0.5g/L、Na2HPO4·12H2O 1.57 g/L、 MgSO4·7H2O 0.1g/L。
3), after exposing nitrogen, it is added to produce in hydrogen culture medium according to the rate of vaccination of 100% by culture fluid during aerobic growth to exponential growth latter stage；Adding xylose in hydrogen culture medium as substrate producing, in the anaerobic reaction (fermentation) of 72h, at interval of the volume being sampled collection hydrogen in 12 hours and content, anaerobic reaction (fermentation) temperature is 35 DEG C.
Total hydrogen output under 2 ~ 8g/L xylose concentration, result is as shown in Figure 1, it can be seen that when substrate xylose concentration respectively 2,4,6,8g/L time, produce hydrogen amount be respectively 72.69mL, 93.73,100.48,104.28mL, xylose rate is respectively 93.45%, 63.45%, 58.3%, 62.5%.Therefore in anaerobic fermentation process, when increasing concentration of substrate, hydrogen output can be accordingly increased, but xylose rate is maximum when 2g/L.
Embodiment 2
Under the conditions of 1% rate of vaccination, the method for hydrogen prepared by xylose, comprises the following steps:
1) takeKlebsiella oxytocaGS-4-08 strain (strain disclosed in CN105524944A), activates with growth medium, it is thus achieved that seed liquor；The formula of growth medium is: yeast extract 5g/L, peptone 10g/L, sodium chloride 10g/L.
2) take seed liquor, be inoculated in the growth medium after sterilizing, antibacterial activation temperature 35 DEG C, rotating speed 180r/min, aerobic growth 12 hours；Carry out again exposing nitrogen treatment；The formula producing hydrogen culture medium (fermentation medium) is: KH2PO4
0.5g/L、Na2HPO4·12H2O 1.57 g/L、 MgSO4·7H2O 0.1g/L。
3), after exposing nitrogen, it is added to produce in hydrogen culture medium according to the rate of vaccination of 1% by culture fluid during aerobic growth to exponential growth latter stage；Adding xylose in hydrogen culture medium as substrate producing, in the anaerobic reaction (fermentation) of 72h, at interval of the volume being sampled collection hydrogen in 12 hours and content, anaerobic reaction (fermentation) temperature is 35 DEG C.
Total hydrogen output under the rate of vaccination of 1%, result, as shown in fig. 2, it can be seen that when xylose concentration is 2g/L, hydrogen output is under 48.8mL, with the same terms, connects the hydrogen output producing hydrogen culture medium that bacterium rate is 100% and reduces 13.8mL.Therefore, connecing bacterium rate is also one of the factor affecting and carrying out producing hydrogen with xylose for substrate, properly increases and connects bacterium rate and can increase the yield of hydrogen.
Claims (7)
1. one kind utilizes the method that hydrogen prepared by xylose, it is characterised in that comprise the following steps:
1) takeKlebsiella oxytocaGS-4-08 strain, cultivates and obtains seed liquor；
2) take in the bacterial growth media after seed liquor is inoculated in sterilizing, at 35 DEG C, then carry out aerobic growth；It is substantial amounts of that cultivation obtainsKlebsiella oxytocaGS-4-08 bacteria culture fluid；
3) carry out exposing nitrogen treatment to product hydrogen culture medium, after exposing nitrogen, be added to produce in hydrogen culture medium according to the rate of vaccination of 100% by bacteria culture fluid during aerobic growth to exponential growth latter stage；
4) anaerobic reaction, collects hydrogen.
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that in step 1), cultivates seed liquor with growth medium.
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that step 2) in, the formula producing hydrogen culture medium is: 0.5g/L KH2PO4、1.57g/L Na2HPO4·12H2O、0.1g/L MgSO4·7H2O。
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that step 2) in, the pH value producing hydrogen culture medium is about 7.0.
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that step 2) in, at 35 DEG C, carry out aerobic growth 12h.
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that in step 3), xylose solution concentration is 2 ~ 8g/L.
The method utilizing xylose to prepare hydrogen the most according to claim 1, it is characterised in that in step 4), more than anaerobic reaction 48h.
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| CN201610320613.2A CN105803002B (en) | 2016-05-16 | 2016-05-16 | A method of hydrogen is prepared using xylose |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114574418A (en) * | 2022-04-08 | 2022-06-03 | 成都理工大学 | Recombinant escherichia coli and hydrogen production application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445810A (en) * | 2007-11-27 | 2009-06-03 | 中国科学院过程工程研究所 | Method for preparing hydrogen by fermenting biologically pretreated straw |
| GB2478791A (en) * | 2010-03-19 | 2011-09-21 | Qteros Inc | Ethanol production by genetically-modified bacteria |
| CN102286538A (en) * | 2011-08-30 | 2011-12-21 | 哈尔滨工业大学 | Method for producing hydrogen utilizing cellulose |
| CN105524944A (en) * | 2015-11-09 | 2016-04-27 | 南京林业大学 | Preparation method and application of microbial flocculant and application of related strains in preparing microbial flocculant |
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- 2016-05-16 CN CN201610320613.2A patent/CN105803002B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445810A (en) * | 2007-11-27 | 2009-06-03 | 中国科学院过程工程研究所 | Method for preparing hydrogen by fermenting biologically pretreated straw |
| GB2478791A (en) * | 2010-03-19 | 2011-09-21 | Qteros Inc | Ethanol production by genetically-modified bacteria |
| US20110230682A1 (en) * | 2010-03-19 | 2011-09-22 | Qteros, Inc. | Microorganisms with inactivated lactate dehydrogenase gene (ldh) for chemical production |
| CN102286538A (en) * | 2011-08-30 | 2011-12-21 | 哈尔滨工业大学 | Method for producing hydrogen utilizing cellulose |
| CN105524944A (en) * | 2015-11-09 | 2016-04-27 | 南京林业大学 | Preparation method and application of microbial flocculant and application of related strains in preparing microbial flocculant |
Non-Patent Citations (3)
| Title |
|---|
| LEI YU 等: "Isolation and characterization of a Klebsiella oxytoca strain for simultaneous azo-dye anaerobic reduction and bio-hydrogen production", 《APPL MICROBIOL BIOTECHNOL》 * |
| 冯燕青 等: "Klebsiella oxytoca发酵木糖生产2 , 3 -丁二醇的研究", 《林产化学与工业》 * |
| 王爱杰 等: "木质纤维素生物转化产氢技术现状与发展趋势", 《生物工程学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114574418A (en) * | 2022-04-08 | 2022-06-03 | 成都理工大学 | Recombinant escherichia coli and hydrogen production application |
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Application publication date: 20160727 Assignee: Suzhou Seaview electromechanical Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: X2019320000045 Denomination of invention: Method for preparing hydrogen by virtue of xylose Granted publication date: 20190621 License type: Common License Record date: 20190823 Application publication date: 20160727 Assignee: Reliance Environmental Technology (Suzhou) Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: X2019320000044 Denomination of invention: Method for preparing hydrogen by virtue of xylose Granted publication date: 20190621 License type: Common License Record date: 20190823 |
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Effective date of registration: 20201125 Address after: Room N6, room 307, 3 / F, room 1, No. 268, Dengyun Road, Yushan Town, Kunshan City, Suzhou City, Jiangsu Province Patentee after: KELEIBOSHI ENVIRONMENTAL PROTECTION TECHNOLOGY (SUZHOU) Co.,Ltd. Address before: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Patentee before: NANJING FORESTRY University |
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