CN105802969A - lncRNA ENST00000581794.1 and preparation, diagnostic agent, medicine or kit and application - Google Patents
lncRNA ENST00000581794.1 and preparation, diagnostic agent, medicine or kit and application Download PDFInfo
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Abstract
The invention relates to the technical field of genetic engineering, and discloses long-chain non-encoded RNA ENST00000581794.1 as well as a kit and an application thereof. The sequence of the long-chain non-encoded RNA ENST00000581794.1 is as shown in SEQ ID NO:1. A long-chain non-encoded expression profile chip technique is used, and a piece of lncRNA ENST00000581794.1 which is remarkably and highly expressed in blood of patients suffering from acute myocardial infarction can be screened through differential analysis, so that study on pathogenesis of acute myocardial infarction is further enriched.
Description
Technical field
The present invention relates to gene engineering technology field, be a kind of long-chain non-coding RNA ENST00000581794.1 and test kit thereof and application, be i.e. lncRNAENST00000581794.1 and preparation or diagnostic agent or medicine or test kit and application.
Background technology
Acute myocardial infarction (acutemyocardialinfarction, AMI) as coronary heart disease (Coronaryheartdisease, CHD) type comparatively serious in, its morbidity is anxious, case fatality rate is high, and people ' s health defines serious threat.The diagnosis of current acute myocardial infarction depends on clinical manifestation, Electrocardiographic dynamic change and serum biomarkers more.How acute myocardial infarction being diagnosed and prevents earlier, is always up the important topic of our research.And along with the Human Genome Project completes the development with biochip technology, the Disease-causing gene of many single-gene disorders is found, and it is applied in the middle of the Clinics and Practices of disease.And AMI is as a kind of disease affected by multiple nature-nurture factor, hereditism's effect in AMI falls ill is not clear and definite yet.
Long-chain non-coding RNA (longnoncodingRNA, lncRNA) is the macromole ncRNA that a class can play regulating and controlling effect in various biological process.It is widely distributed, and length is typically greater than 200 bases, due to lack effective open reading frame (ORF) and without or seldom have the ability of encoding proteins.As a uncharted field in molecular biology, lncRNA is the effect that the form with RNA plays that in the multiple aspects such as epigenetics, transcriptional control, post-transcriptional control controlling gene is expressed.As the important component part of mammalian transcription group, the function of lncRNA needs to further investigate at present.Originally lncRNA is considered as the by-product that RNA polymerase II is transcribed, and is subgenomic transcription " noise ", does not have biological function.But result of study in recent years shows, lncRNA important function in cell normal physiological activity and participate in multiple disease and develop.LncRNA can implement its function by Genomic Imprinting, chromatin reconstruct, the mechanism such as regulation and control, mRNA degraded and translational control of shearing.
Summary of the invention
The invention provides the preparation of a kind of long-chain non-coding RNA ENST00000581794.1 or diagnostic agent or medicine or test kit, overcome the deficiency of above-mentioned prior art, it can effectively solve acute myocardial infarction as a kind of disease affected by multiple nature-nurture factor, but the problem that the effect that hereditism is in acute myocardial infarction is clear and definite not yet, present invention discover that the generation of its expression or dysfunction and human cardiovascular disease and develop closely related, long-chain non-coding RNA is expected to become cardiovascular disease diagnosis, the mark of prediction prognosis, simultaneously also treatment for cardiovascular diseases provides new target spot.
One of technical scheme is realized by following measures: a kind of long-chain non-coding RNA ENST00000581794.1 of high expressed in patients of acute myocardial infarction blood, it is characterised in that the sequence of this long-chain non-coding RNA ENST00000581794.1 is such as shown in SEQIDNO:1.
Further optimization and/or improvements to foregoing invention technical scheme are presented herein below:
Above-mentioned long-chain non-coding lncRNAENST00000581794.1 is the total length of ENST00000581794:1.
The two of technical scheme are realized by following measures: the preparation of long-chain non-coding RNA ENST00000581794.1 or diagnostic agent or medicine or test kit in a kind of vitro detection blood, said preparation or diagnostic agent or medicine or test kit include specific primer to, standard DNA template, PCR reactant liquor, wherein, specific primer is to including forward primer and downstream primer, the nucleotides sequence of forward primer is classified as SEQIDNo:2, and the nucleotides sequence of downstream primer is classified as SEQIDNo:3.
Be presented herein below to foregoing invention technical scheme two further optimization and/or improvements:
This test kit above-mentioned is fluorescent quantificationally PCR detecting kit.
Above-mentioned specific primer to for SYBRGreen, Taqman probe, molecular beacon, double cross probe, combined probe detection.
Above-mentioned reactant liquor is PCR reactant liquor is fluorescence quantitative PCR reaction solution.
Above-mentioned fluorescence quantitative PCR reaction solution includes dNTP, Mg2+, Taq enzyme.
Above-mentioned also include fluorescent dye.
The three of technical scheme are realized by following measures: a kind of detection is according to the preparation of the long-chain non-coding RNA ENST00000581794.1 one of technical scheme Suo Shu or diagnostic agent or medicine or test kit, it is characterized in that carrying out in the steps below: the first step, extract blood total serum IgE；Second step, prepares cDNA；3rd step, quantitative amplification lncRNAENST00000581794.1.
The four of technical scheme are realized by following measures: a kind of application being used in preparation according to the long-chain non-coding RNA ENST00000581794.1 one of technical scheme Suo Shu detecting the test kit of acute myocardial infarction or the novel targets as detection acute myocardial infarction medicine.
nullThe present invention utilizes long-chain non-coding chip of expression spectrum technology，Pass through variation analysis，Screen a lncRNAENST00000581794.1 for significantly high expression in patients of acute myocardial infarction blood，Enrich the research of acute myocardial infarction mechanism further，Simultaneously，The reagent of vitro detection acute myocardial infarction related gene of the present invention and long-chain non-coding RNA ENST00000581794.1 and the test kit of vitro detection acute myocardial infarction related gene of the present invention and long-chain non-coding RNA ENST00000581794.1 can be used in the detection of the expression of long-chain non-coding RNA ENST00000581794.1 of the present invention，The screening results of acute myocardial infarction is known by the testing result of the expression of long-chain non-coding RNA ENST00000581794.1 of the present invention，New direction is provided for diagnosing and treating of acute myocardial infarction.
Accompanying drawing explanation
Accompanying drawing 1 is long-chain non-coding RNA chip of expression spectrum detection ENST00000581794.1 detected signal value in normal healthy controls group with acute myocardial infarction group in the present invention.
Accompanying drawing 2 be the sequential design in the present invention for long-chain non-coding RNA ENST00000581794.1 a pair specific primer pcr amplification after row agarose gel electrophoresis test primer effect.
Accompanying drawing 3 is that in the present invention, qRT-PCR detects ENST00000581794.1 relative expression quantity in normal healthy controls group with acute myocardial infarction group.
Detailed description of the invention
The present invention, not by the restriction of following embodiment, can determine specific embodiment according to technical scheme and practical situation.
In the present invention, for the ease of describing, the Butut mode that the description of the relative position relation of each parts is all according to Figure of description 1 is described, as: the position relationship of upper and lower, left and right etc. is based on what the Butut direction of Figure of description was determined.
The present invention utilizes long-chain non-coding chip of expression spectrum technology, pass through variation analysis, screen a lncRNAENST00000581794.1 for significantly high expression in patients of acute myocardial infarction blood, its transcript regions is positioned at No. 7 chromosomes, and original position is 30,606,019-30,634,385, total length 28367bp.Compared with the blood content of Healthy People, lncRNAENST00000581794.1 is significantly high expression in patients of acute myocardial infarction blood, and is further characterized by lncRNAENST00000581794.1 and is significantly higher than Healthy People in patients of acute myocardial infarction blood in the fluorescent quantitation of sample is tested.This novel lncRNAENST00000581794.1 is by the research of abundant acute myocardial infarction mechanism further, and also early diagnosis and Prognosis scoveillance for acute myocardial infarction provide new blood molecules mark and therapy target.
Each 3 of the blood preparation of patients of acute myocardial infarction provided by the invention and Healthy People, totally 6 example.After extracting total serum IgE by step needed for the Trizol reagent (article No. 15596-026) of ABI company, the chip product (AgilenthumanlncRNA+mRNAArrayV4.0) adopting Beijing Bo Ao biological engineering company limited detects, filtering out a lncRNAENST00000581794.1 for notable low expression in patients of acute myocardial infarction blood, its nucleotide sequence is such as shown in SEQIDNo.1.Later stage is through carrying out quantitative fluorescent PCR checking to sample, it has been found that in 6 samples, patients of acute myocardial infarction is significantly higher than the expression of Healthy People lncRNAENST00000581794.1.LncRNAENST00000581794.1 is as the novel targets of acute myocardial infarction, and clinical treatment and drug development for acute myocardial infarction are provided fundamental basis.
Embodiment 1, a kind of long-chain non-coding RNA ENST00000581794.1 of high expressed in patients of acute myocardial infarction blood, the sequence of this long-chain non-coding RNA ENST00000581794.1 is such as shown in SEQIDNO:1.
Embodiment 2, as the optimization of embodiment 1, long-chain non-coding lncRNAENST00000581794.1 is the total length of ENST00000581794:1.
Embodiment 3, the preparation of long-chain non-coding RNA ENST00000581794.1 according to embodiment 1 and embodiment 2 or diagnostic agent or medicine or test kit in a kind of vitro detection blood, said preparation or diagnostic agent or medicine or test kit include specific primer to, standard DNA template, PCR reactant liquor, wherein, specific primer is to including forward primer and downstream primer, the nucleotides sequence of forward primer is classified as SEQIDNo:2, and the nucleotides sequence of downstream primer is classified as SEQIDNo:3.
Embodiment 4, as the optimization of embodiment 3, this test kit is fluorescent quantificationally PCR detecting kit.
Embodiment 5, as the optimization of embodiment 3 or embodiment 4, specific primer to for SYBRGreen, Taqman probe, molecular beacon, double cross probe, combined probe detection.
Embodiment 6, as the optimization of embodiment 3, embodiment 4 and embodiment 5, PCR reactant liquor is fluorescence quantitative PCR reaction solution.
Embodiment 7, as the optimization of embodiment 6, fluorescence quantitative PCR reaction solution includes dNTP, Mg2+, Taq enzyme.
Embodiment 8, as the optimization of embodiment 3, embodiment 4, embodiment 5, embodiment 6 and embodiment 7, this test kit also includes fluorescent dye.
The using method of the dye class PCR kit for fluorescence quantitative of this vitro detection acute myocardial infarction, quantitative fluorescent PCR system:
Forward primer, downstream primer each 0.25ul (10uM)；DNA profiling cDNA0.5ul；PowerSYBRGreenPCRMaster (2 ×) 5ul, adds Nuclease-FreeWater4ul, total capacity 10ul.Quantitative fluorescent PCR program: the first step 95 DEG C 10 minutes；Second step, 95 DEG C of 15S, 60 DEG C 1 minute；3rd step, 95 DEG C of 15S, 60 DEG C 1 minute, 95 DEG C of 15S, totally 40 circulations.
Embodiment 9, the method for a kind of detection long-chain non-coding RNA ENST00000581794.1 according to embodiment 1 and embodiment 2, carry out in the steps below: the first step, extract blood total serum IgE；Second step, prepares cDNA；3rd step, quantitative amplification lncRNAENST00000581794.1.
Described method specifically includes following steps:
One, extract blood total serum IgE: extract total serum IgE according to reagent needed for the Trizol reagent (article No. 15596-026) of ABI company and step, then with 7300realtimePCRsystem nucleic acid quantification instrument quantitative (AppliedBiosystemsAB) quantitative extracted purity and concentration.
Two, sample cDNA is prepared: adopt Beijing Tian Gen biochemical technology company FastQuantcDNA the first chain synthetic agent box test kit (article No. KR106) the total serum IgE reverse transcription synthesis cDNA to extracting.
1, template ribonucleic acid is thawed on ice；5×gDNABuffer、FQ-RTPrimerMix、10×FastRTBuffer、RNase-FreeddH2O thaws in room temperature (15-25 DEG C), is immediately placed on ice after defrosting.Every kind of solution vortex oscillation being mixed before using, brief centrifugation remains in the liquid of tube wall to collect.
2, opening secondary structure, reaction system and condition are as shown in table 1；
The removal system of the genomic DNA of said components is prepared mixed liquor, thoroughly mixes.Brief centrifugation, is placed in 42 DEG C, hatches 3min.It is subsequently placed in and places on ice.
3, reverse transcription reaction, reaction system and condition are as shown in table 2；
By the Mix in reverse transcription reaction, it is added in the reactant liquor of gDNA removal step, fully mixes.42 DEG C, hatch 15min.95 DEG C, being put on ice after hatching 3min, the cDNA obtained can be used for subsequent experimental or cryopreservation.
Three, amplification lncRNAENST00000581794.1: adopt the PowerSYBRGreenPCRMasterMix fluorescence quantitative kit (article No. 4367659) of ABI company, carry out fluorescent quantitative PCR with the cDNA of reverse transcription for template.
Quantitative fluorescent PCR system is as shown in table 3；
Quantitative fluorescent PCR program: the first step 95 DEG C 10 minutes；2nd step, 95 DEG C of 15S, 60 DEG C 1 minute；3rd step, 95 DEG C of 15S, 60 DEG C 1 minute, 95 DEG C of 15S, totally 40 circulations.
Embodiment 10, a kind of long-chain non-coding RNA ENST00000581794.1 according to embodiment 1 and embodiment 2 is used for detecting the test kit of acute myocardial infarction or being used as to detect the application of the novel targets of acute myocardial infarction medicine in preparation.
For the method detecting acute myocardial infarction, said method comprising the steps of:
The first step, extracts blood total serum IgE；
Second step, prepares sample cDNA；
3rd step, expands lncRNAENST00000581794.1, and judges according to relative quantification result.
Embodiment 11: the lncRNA chip expression analysis of patients of acute myocardial infarction and healthy human blood.
One, material and method
1. material
Blood sample comes from patients of acute myocardial infarction and each 3 of healthy human blood's sample.
2. method
(1) extraction of patients of acute myocardial infarction and healthy human blood's sample total serum IgE: " preparation process of blood sample for gene microarray analysis " that provide according to Bo Ao Bioisystech Co., Ltd extracts patients of acute myocardial infarction and healthy human blood's sample total serum IgE.Specific experiment material includes: lymphocyte separation medium (TBD biotech development center, Tianjin)；Sterile saline (common 0.9% normal saline sterilizing)；Trizol reagent (Invitrogen company).
(2)The detection of lncRNA+mRNA chip of expression spectrum is initial with the total serum IgE of detected sample, carries out amplification in vitro and fluorescent labeling, and labeling process adoptsBiochip common tags test kit.Mainly comprise the steps:
1) reverse transcription synthesizes the first chain cDNA: initiate with TotalRNA, T7Oligo (dT) Primer and T7-random primer containing T7 promoter sequence, both can in conjunction with the mRNA with poly (A), can also other, without the RNA of poly (A), uses the first chain EnzymeMix to synthesize the first chain cDNA in conjunction with except rRNA.
2) the second chain DNA is synthesized: with the second chain EnzymeMix, the RNA chain in DNA-RNA heterozygote is converted into the second chain cDNA, synthetic dsdna.
3) in vitro transcription synthesis cRNA: with the second chain cDNA for template, utilizes T7EnzymeMix to synthesize cRNA.
4) cRNA purification: use RNA purification column purification cRNA, removes the salt in reaction, the reagent such as enzyme, and cRNA is carried out quantitatively, Quality Control.
5) reverse transcription: with cRNA for template, RandomPrimer is primer, and CbcScript II enzyme carries out reverse transcription.The cDNA that purification recovery reverse transcription obtains is also quantitatively.
6) labelling: with the cDNA product of reverse transcription for template, RandomPrimer is primer, with KlenowFragment enzymatic synthesis cDNA complementary strand and mix the dNTP (Cy3-dCTP, Cy5-dCTP) with fluorophor, purification quantitative mark product.Namely chip hybridization is can be used for fluorophor DNA.
7) chip hybridization and cleaning: take 1 μ LcDNA and be dissolved in hybridization solution, 45 DEG C of hybridized overnight.Taking-up chip is washed dry instrument at rich SlideWasher8 chip difficult to understand and is carried out, and cleaning procedure is as follows: washing liquid I:0.2%SDS, 2 × SSC, and 42 DEG C of 120S clean 2 times.Washing liquid II:0.2%SDS, 2 × SSC, 42 DEG C of 80S cleans 3 times.
After cleaning procedure completes, centrifuge dripping, to be scanned.
8) chip scanning, data analysis, differential gene screens: use Agilent chip scanner (G2565CA) that the chip after cleaning is scanned, and obtains hybridization picture.Use AgilentFeatureExtraction (v10.7) software that hybridization picture is analyzed and extracts data.Then use AgilentGeneSpring software data to be normalized and variation analysis, and carry out differential gene screening with GeneSpringGX software.
Two, result
Long-chain non-coding chip of expression spectrum detection ENST00000581794.1 detected signal value in normal healthy controls group with acute myocardial infarction group is as shown in Figure 1.Chip examination finds the lncRNAs of a plurality of up-regulated and down-regulated expression.Wherein lncRNAENST00000581794.1 demonstrates and expresses notable rise in patients of acute myocardial infarction, and Fc value is 2.067 times, wherein, upper it can be seen that P is less than 0.05 by figure, has statistical significance.In view of it is likely to exist in patients of acute myocardial infarction specific expressed, the present invention adopts the sample of chip detection to carry out the repeated authentication of index by following example.
Embodiment 12:qRT-PCR repeated authentication lncRNAENST00000581794.1 differential expression in patients of acute myocardial infarction and Healthy People.
One, experiment material
Choose the sample of chip testing, the differential expression of lncRNAENST00000581794.1 is carried out qRT-PCR checking.
Two, experimental technique and result
1. primer specificity is identified
(1) design of specific primer: extract the relevant transcript sequence of lncRNAENST00000581794.1 from Ensemble data base, and design primer by the sequence according to transcript by the design of primers instrument (PrimerBLAST) of NCBI；
Primer sequence after design is as follows:
Forward primer: SEQIDNo:2
Downstream primer: SEQIDNo:3
(2) patients of acute myocardial infarction and healthy human blood are extracted total serum IgE according to reagent needed for the Trizol reagent (article No. 15596-026) of ABI company and step, then with 7300realtimePCRsystem nucleic acid quantification instrument quantitative (AppliedBiosystemsAB) quantitative extracted purity and concentration.
Adopt Beijing Tian Gen biochemical technology company FastQuantcDNA the first chain synthetic agent box test kit (article No. KR106) the total serum IgE reverse transcription synthesis cDNA to extracting.
Template ribonucleic acid is thawed by the first step on ice；5×gDNABuffer、FQ-RTPrimerMix、10×FastRTBuffer、RNase-FreeddH2O thaws in room temperature (15-25 DEG C), is immediately placed on ice after defrosting.Every kind of solution vortex oscillation being mixed before using, brief centrifugation remains in the liquid of tube wall to collect.
It is as shown in table 4 that second step opens secondary structure, reaction system and condition,
The removal system of the genomic DNA of said components is prepared mixed liquor, thoroughly mixes.Brief centrifugation, is placed in 42 DEG C, hatches 3min.It is subsequently placed in and places on ice.
3rd step reverse transcription reaction, reaction system and condition are as shown in table 5:
By the Mix in reverse transcription reaction, it is added in the reactant liquor of gDNA removal step, fully mixes.42 DEG C, hatch 15min.95 DEG C, being put on ice after hatching 3min, the cDNA obtained can be used for subsequent experimental or cryopreservation.
(3) amplification lncRNAENST00000581794.1: adopt the PowerSYBRGreenPCRMasterMix fluorescence quantitative kit (article No. 4367659) of ABI company, carry out fluorescent quantitative PCR with the cDNA of reverse transcription for template.
Quantitative fluorescent PCR system is as shown in table 6；
Quantitative fluorescent PCR program: the first step 95 DEG C 10 minutes；2nd step, 95 DEG C of 15S, 60 DEG C 1 minute；3rd step, 95 DEG C of 15S, 60 DEG C 1 minute, 95 DEG C of 15S, totally 40 circulations.
Electrophoresis detection, selects 0120000Marker (Beijing CoWin Bioscience Co., Ltd., article No. CW0632) result as shown in Figure 2: amplified fragments size is identical with expection, amplified production only one of which band.This primer pair meets above-mentioned standard.The specific primer of upstream, its sequence is shown in sequence table SEQ IDNo.2, the specific primer in downstream, and its sequence is shown in sequence table SEQ IDNo.2.
2. the preparation of standard DNA template
According to lncRNAENST00000581794.1 base sequence (its nucleotide sequence is such as shown in sequence table SEQ IDNo.1), entrust the raw work synthesis in Shanghai.
Sampling 2ul synthetic product, is connected to Puc-TTA cloning vehicle (Beijing CoWin Bioscience Co., Ltd., article No. CW0801), is transformed into subsequently in DH5a competent cell.By the specific primer screening positive clone that sequence is SEQIDNo.2 and SEQIDNo.3, extract plasmid DNA, plasmid DNA is quantitative with 7300realtimePCRsystem nucleic acid quantification instrument, and (standard DNA template concentration range is 10 as standard curve to do 10 times of serial dilutions2-106Copy/ul).
3. sensitivity experiments is by standard
Being diluted in proportion by standard DNA template plasmid is 102、103、104、105、106Copy/ul, carries out fluorescence quantitative PCR detection sensitivity.Concentration limit is 102Copy/ul.
4. synthesis cRNA template
Take each 3 of the total serum IgE of above-mentioned acute myocardial infarction and Healthy People, adopt Beijing Tian Gen biochemical technology company FastQuantcDNA the first chain synthetic agent box test kit (article No. KR106) the total serum IgE reverse transcription synthesis cDNA to extracting.
Template ribonucleic acid is thawed by the first step on ice；5 × gDNABuffer, FQ-RTPrimerMix, 10 × FastRTBuffer, RNase-FreeddH2O thaw in room temperature (15-25 DEG C), are immediately placed on ice after defrosting.Every kind of solution vortex oscillation being mixed before using, brief centrifugation remains in the liquid of tube wall to collect.
It is as shown in table 7 that second step opens secondary structure, reaction system and condition:
The removal system of the genomic DNA of said components is prepared mixed liquor, thoroughly mixes.Brief centrifugation, is placed in 42 DEG C, hatches 3min.It is subsequently placed in and places on ice.
3rd step reverse transcription reaction, reaction system and condition are as shown in table 8:
By the Mix in reverse transcription reaction, it is added in the reactant liquor of gDNA removal step, fully mixes.42 DEG C, hatch 15min.95 DEG C, being put on ice after hatching 3min, the cDNA obtained can be used for subsequent experimental or cryopreservation.
5. fluorescence quantitative PCR detection lncRNAENST00000581794.1
Adopt the PowerSYBRGreenPCRMasterMix fluorescence quantitative kit (article No. 4367659) of ABI company, carry out fluorescent quantitative PCR with the cDNA of reverse transcription for template.
Quantitative fluorescent PCR system is as shown in table 9:
Quantitative fluorescent PCR program: the first step 95 DEG C 10 minutes；2nd step, 95 DEG C of 15S, 60 DEG C 1 minute；3rd step, 95 DEG C of 15S, 60 DEG C 1 minute, 95 DEG C of 15S, totally 40 circulations.
QRT-PCR detects ENST00000581794.1 relative expression quantity in normal healthy controls group with acute myocardial infarction group as shown in Figure 3, the relative quantification formula according to qRT-PCR: 2-△△ctCalculate lncRNAENST00000581794.1 expression in patients of acute myocardial infarction (AMI) and Healthy People (N) respectively, patients of acute myocardial infarction shown in result (AMI) compares with the lncRNAENST00000581794.1 expression of Healthy People, and they are 2 years old-△△ctValue respectively 1.2790,1.4681,0.7556, its meansigma methods is 1.3735.These results suggest that, lncRNAENST00000581794.1 is general high expressed in patients of acute myocardial infarction blood, and this is consistent with foregoing lncRNA chip results ENST00000581794.1 rise 2.067219 times, wherein, by in figure it can be seen that P is less than 0.05, there is statistical significance.Therefore, lncRNAENST00000581794.1 can as the new blood biomarker of Diagnosis of Acute Myocardial Infarction, for the examination of acute myocardial infarction.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can increase and decrease non-essential technical characteristic according to actual needs, meet the demand of different situations.
Table 1
| Reagent | Consumption |
| 5×gDNA Buffer | 2ul |
| Total serum IgE | 1-2ug |
| DEPC water | Complement to 10uL |
Table 2
| Reagent | Consumption |
| 10×Fast RT Buffer | 2ul |
| RT Enzyme Mix | 1ul |
| FQ-RT Primer Mix | 2ul |
| RNase-Free ddH2O | Supply 10 μ l |
Table 3
| Reagent | Consumption |
| Power SYBR Green PCR Master(2×) | 5ul |
| CDNA sample | 0.5ul |
| Forward Primer(10μM) | 0.25ul |
| Reverse Primer(10μM) | 0.25ul |
| Nuclease-Free Water | 4ul |
| Total capacity | 10ul |
Table 4
| Reagent | Consumption |
| 5×gDNA Buffer | 2ul |
| Total serum IgE | 1-2ug |
| DEPC water | Complement to 10uL |
Table 5
| Reagent | Consumption |
| 10×Fast RT Buffer | 2ul |
| RT Enzyme Mix | 1ul |
| FQ-RT Primer Mix | 2ul |
| RNase-Free ddH2O | Supply 10 μ l |
Table 6
| Reagent | Consumption |
| Power SYBR Green PCR Master(2×) | 5ul |
| CDNA sample | 0.5ul |
| Forward Primer(10μM) | 0.25ul |
| Reverse Primer(10μM) | 0.25ul |
| Nuclease-Free Water | 4ul |
| Total capacity | 10ul |
Table 7
| Reagent | Consumption |
| 5×gDNA Buffer | 2ul |
| Total serum IgE | 1-2ug |
| DEPC water | Complement to 10uL |
Table 8
| Reagent | Consumption |
| 10×Fast RT Buffer | 2ul 8 --> |
| RT Enzyme Mix | 1ul |
| FQ-RT Primer Mix | 2ul |
| RNase-Free ddH2O | Supply 10 μ l |
Table 9
| Reagent | Consumption |
| Power SYBR Green PCR Master(2×) | 5ul |
| CDNA sample | 0.5ul |
| Forward Primer(10μM) | 0.25ul |
| Reverse Primer(10μM) | 0.25ul |
| Nuclease-Free Water | 4ul |
| Total capacity | 10ul |
Sequence table
<110>No.1 Hospital Attached to Xinjiang Medical Univ.
<120>long-chain non-coding RNA ENST00000581794.1 is as the detection of the blood molecules mark of Diagnosis of Acute Myocardial Infarction and application thereof
<130>
<160>
<170>
<210>1
<211>28367
<212>DNA
<213>Homosapiens
<400>1
1agacgtaagtgattaacttcttctaatccataactaaggtgtgaagaccttcttctggag
61cctcagtaaatgtattgaatctagatgggtccaagtgctggggtgattacccttatcaca
121tctcctgctaaatcacggaggtttgaggagttcatttagacctccaataaaacttgtgtc
181ttcagacccccagtaaaaacttttcttttcttttttttttttttgagatggagtctcact
241ctgttgcccaggctgaagtgcagtggtgtgatcccaggtcactgcaacctccgctttctg
301ggttcaagcgattctcctgccccagcctcctgagtagttgggactacaggctcccgccac
361catgcttggctcattttggtacttttagtagagacaggagtttcaccatgttggccagga
421ggctggtctcaaactcctgacctcaggtgatctgcctgcctcagcctcccaaagtgctga
481gattataggtgtgagccaccgtacccagccaataaaaacttacttaatcctaaatgggtc
541tggttaagaattccttcctcctcttgtcatggttcaaggcacaggaaagacttaggcaaa
601aacttgtggtgggcttttgttctattccagcctttgtataaggttactggctctttcaac
661ttttaatatttaacttaaccactcagtcagcgctgaaacagttgttacagaggcctgcct
721gttcagctgctagagagacctaggatgccacagtgtggatgggtagaatttgggatggga
781agtagactaaggagggaattttcagccaagggtacagtgtccttggagagaatttgatgt
841atacattttaactgttccagcctcaactgatgcttggttcatctcccaggactctgcttt
901ccccacagtcctctccaatgggtgatccttcatccctgggctgctctagaccacaacctc
961tgtgtcttgggtaaaaccttgctccactgaagttcttggcacttccagtctagtcagtgc
1021ccaccatctgccaacctcctactgtctgccttcatcagtgaagaccgtaatcataggctc
1081tcagcttccttcccactcagaagcctcccgtgaccctggatgacatcaacacattcccat
1141gaagggccgtactaacacccaagcctaccctattgcttcagtctcttgacctacaatcat
1201ccccatctccacccacattaatgacaattctcatggcaaataatgacaatttgtcattat
1261tcagaaatgtaccgtttctgagatataggtttattatccctaatctgaaaatctgaaatt
1321tgaaatgctctaaaatccaaaaccttttgagcaccattgtgatgctcaaaggaaacaatc
1381attggagaattttggatttcacgttttcagattaggggtgctgaacctgtataatgcaaa
1441tattccaaaatctgaaaacatctgaaatctgaaacacttctggtcccaagcattttggat
1501aagaaatatgcaaccagtattaccttcaacatcccactctctctaactgtaaacttccat
1561ccttctagctctctaatttacttatttcttcacttctccttgtaaatgttaaaggaaaat
1621aaatcttgggacctcaaagtcactaagccaaagggaaaagtcaatctggaaactgcacgc
1681aaacctgcctctcattatattcctaaacaagatagctagacagaactcaaagtcatccct
1741ctgctcacgtgagacaaatgcatagctgattgcttcctttgccctgttgtttcactgagc
1801caggctaaggcttaagtgattagtcctgtaaattgtgtattcagtgaaaggcttattaga
1861aactcaaaagaatgcaactgtttttctcttatctacctatcacctggaagccccctccct
1921gtttccagtcatcccacctttttggaccacctgatatatgtcttaacatatattgattga
1981tatctcatgtctccctaaaatgtgtaaaaccaagctgtgccccaaccaccttgcacacgt
2041caacagaacctcctgaggctatgtcatggatgtgtccttaaccttggcaaaataaacttt
2101ctaaattgactgagacccatatcagatattttgtgttcacaattggtaatcacagaggga
2161ttctgagtgaagatgcctcgatctttgacaaatctcctatcagtgcgtggtagcagcctg
2221agctgtctttatggctgaaaccaataggacaattggctgaggcctgggagccaccccacc
2281cccagagaatccctgatctccccaaatttgttgagatctgaagtttattttgctgtgcaa
2341ctccttttttggagttttacttgcttccaacaaggaaggcaaattttcctgcgtccatga
2401tgatggaaggcaggtaactcctttctggagtttgagctcgtttccagcagggaagatgag
2461tttcagttttttcctgcttctaggatgggagagagcagacttcagcctgagactcattac
2521taggtaagtagctgaattggagttttgtcttggctagagttaagactaacagccagctgg
2581tcttaatttctccttactattagagcactcagtgaccacataaattgtgtgattgtttgt
2641ttgtttcacttaactgtttttgttgttgttgttgttgctgtttgtttctgtttcttgttg
2701ttttcatctttttcccattgggtttgaccaactctatccaacttgatcaaatccgaagga
2761aaattccaaattatggagaacaagacctctgaattggctaaattcctgcaactgaaaaag
2821aaaaaagaaaaaaacagctagcaaaaggaaaaaaaaaatttttttgactttgactacctt
2881aggggctttatttacataacaaggccatcttttgctaaccaggccaaactgaaagagcaa
2941tggttgtcccccagcactgcagtttggaaactaagattctgtccttattttcatcatgac
3001tgcctaagtttggttcctaaatcaagccctttctggtttggtatttgcattacttttgaa
3061atatcagcagtttgtactagctaaaatacagtaatgagatttgaaggttttttttttaag
3121gagcttagtggctaaaagcttaaataaaagctaacatccaaggtgtgtgtgtgtgtgtgt
3181gtgtgtgtgtgtagttaaggccttttttatttctctccttcaactttttgagcaaaagtt
3241tttttcttctccgtcaaccaaattctgttttcttcattttacttctgctgcctcttcttt
3301ctcttgctaccctgtgctgcatgaggaatctaaaatagtttctaacaccctgggattcct
3361taaagaaaacagagaaggcactagactcccttttggggagcaacctctgtttctccttat
3421ggaactcaaagagtgtaaaagagaaaatttcgtctcagatcttacagtgcttccttttgt
3481atagtgttacctggtttctttctttcttcttttttttttttttttaactaaaataattat
3541tttagtcaaagagtaacaacagaggttactgttgggtgtttaaagttaggaagagagtag
3601ttcagacacttagagaaacatttttgttaaaaaaaaaaaaaaggagagtgcactcataaa
3661accatcacatggtcttagcctcataataattctctttttggagacccggaattcagtgtg
3721ggctctgcccagagctcagagatccagttaaaaggcagactaaatttagaactacttata
3781taaataaagtcagtcttctaatataatcctatgatagatttctataattttatgtttgat
3841ttggcattcgtttttaatctcctcctactagtgagaggtgaaaccagctggacttcctgg
3901gtcgagtggggacttggggaacttttctgtctagctagaggattataaacacaccaatca
3961gtgctctgtgtctagctaaaggattgtaaatataccaatcagcactctgtaaaaacgcac
4021caatcagcgctctgtgtctagctaaaggattgtaaatgcaccaagcagcactctataaca
4081atgcaccaatcagtgctctgtgtctagctaaaggattgtaaatgcaccaagcagcactct
4141ataacaatgcaccaatcagtgctctgtgtctagctaaaggattgtaaatgcaccaatcag
4201cactctgtggctaaaggattgtaaatgcaccaatcagcactctgtaaaaatgcaccaatc
4261agcactctgtgtctagctaaaggattgtaaatgcgccaatcagcactctgtaaaatgcac
4321caatcagcactctgtgaaatggaccaatcagtgctctgtaaaatggaccaatcaccagga
4381cgtgggtggggacgaataagggaataaaagctggccaccacagccagcagcatcaacccg
4441ctcaggtccctttccatgctgtggaagctttgttctttcgctcttcataataaatcttgc
4501tgctgctcactctttgggtccacactacctttaattgctgtaacactcaccatgagggtc
4561tgcagcttcattcctgaagtgagaccacaaacccaccagaaggaacaaacaaccctggac
4621acgccacctttaagagctgtaacactcactgcaaggtccgaggcttctttcttgaagtca
4681gagagaccaagaacccaccagaaggaataaattctggaaacactagcaccagactctttc
4741tctctgtaccttgagatgtaaatttcactatctgatttttttcatctaagagttccttta
4801atatgcaaatttagggttatcttagctgacaactgcctagggtaataagatcaagaaatt
4861agaagtctagaactggaggaaaaaaagaggtcttgtgaaatgataagctctacttctatc
4921tgcatgtataatacatctatgtatttatgtgtcatgtatatgatgtttcactactagaaa
4981catgtaaaagtgctctaattaattggcttaaagaaaaaaaggggctttaaatcaaatact
5041gtatcagagaaaatagagattttaagccaaatgtcttttcaagttcatgtgacttaagta
5101tatctttaataaataagctggttttgaaagtagtcatgaaataaaattagaattagaatg
5161gcttcagaattgtcaacatacactattgtttaaatttattggaacagtggtttcatattt
5221atctctgctagatgccaaaatttggcatgagggttataaaacaatgaatgcagcccaaag
5281gaaaattatctctatgtaaaatttcataaataaggcatttaatattgtttagttaatgaa
5341aacagctaaatcctaagttattggcaaaaaaaaaaaaaaaaaaaaaaaaaaccatttttc
5401taaccttaagttttttacttaggaaaacctgaaattcgcagtctataaaaatgattaact
5461gggaaataactttcaatgataactctcagttttcaaaagtaatctgagtaaactattaaa
5521aaaattaattaggtaaatgtaatggaataaatgcttgtgattaaacttgttatataattt
5581agaatctaaaattatattaagttaaataataggtactcattaaacatctgggtcatttcc
5641aatttaaaatattaggccaggtgcagtggctcacacctgtaatcccagcactttgggagg
5701ctgaggcagacagatcatagtcaggagatcgagatcatcctggctaacacagtgaaaccc
5761cgtctctactaaaaatacaaaaaattagccaggcgtggtggtgtgcacctctagtcccag
5821ctaccctggaggctgaggcaggaggatggcgtgaacccgggaggcagaacttgcagtgag
5881ccaagattgtgccactgcactccagcctgggtgacagagcaagactctgtctcaagaaaa
5941aaaaaaaattacagaaaaatatttttctaaaaaaagaaatgtgttcttatttaaaggaaa
6001ataattttttatctaattcaaggctatttaaaacttatgaaacaaggtaaaagtaatcgt
6061taaataaaagcaatgtaaagaaagttataaaaggtatttctggcaagaaaagttagaagg
6121aagataattgtatatgagaaatattcttgtatggtaaaattttggcctagaataaaacga
6181ctggttgtttaagaaagagggatgttcaggacaaaccagaaagttcaagtgttactggta
6241gaggatcttgactatgagtcatccaggttcttggtgttttgaacaaagaattggacaaaa
6301cacacaaagcaacgaaggaatgaagcaatgaaagcacaaatttattgaaatgaaagtaca
6361cttcacaaagtgggagtaggctccagcaagcagctcaagagcactggttacagaattttc
6421tggggtttacatacccgctagaggtttcccattggttacttggttacaccctatgtaaac
6481gaaggagtagcccatggccagtctgattggttgtgggaggggaccaatctgaggtacttt
6541ccattttttatctgcaatgcagtgcaaagggagtagcctctgatccttttgttacttggg
6601tgtggagaggtggggttttccttttcattcaatcctagaaactcagcacaaatcagcctt
6661aggttccctgtctccagaccctattctcctgcctcattttcccctgagagatgtgatccc
6721cataaatctttctgggaggcagagggacccatggtctttcttccataactacttcatgct
6781gacttggggcacagtccctacctactggggattacagaactctcgccctgctctgtctag
6841tggagacaaggttacttcttggtggccggggttgacatcattatctgaaactgactggtg
6901gccctgctgcattatcataatggtctctaggcaagaagaaataaatctggttaaaagatt
6961taacaaacatgatccaaaagccaaggcaagtataataattaatagtggactggccagagg
7021aaggagatgtgagccccaaattagcattttgactaggcgccccatgactcagacagctgt
7081tgtcatatcttatggtaccaatcggctagtttttgggtggcatcccttactaatcctgtt
7141tggttgacataaaaacagcattcttcctctaggaaaaagacataagccaccttcttcagc
7201atttaggagatccagtccccttctattttacaaagccaccactgccaaagactctatctg
7261attttgtaaggtgatgatacttttgggcaatgtcacctaagctttccataaaatccttgg
7321acaagcattggtaataggatagggaaactgcaaggctgctaactctcattcctactcctg
7381ctgttattcctagctctaccgaaagtggtatcagttggatggctcatttgtgtctggtgg
7441ttgcagttaaaagtataatgaaggattggttgttgggagctatattaatttcgggggcta
7501aataaacaactgtataggttccagtccaattggctggtaaaacataagtaggaactggtc
7561ccacacgggaaaaagagtccttgtttttcaagacaaaattgttttctatggtgaacatgt
7621gggttagcttgttgttttcactttcccaagttattaaagtccctggtaaggtggccaagg
7681gaaaatgtgaacacagctagtgggcctaccttaacggtatttagactatatatttaaagt
7741gtccttgactagggatggtttccataaaaactgtacaggttttctaaatgatgcagcttg
7801ggtaactgcatagcttacatgatcatgtgagggtttaatctgtatggtgtagttacattg
7861gtgactttagatgtcccagggcctgaccaaaattttggcttctctcaatacaaagtgttg
7921cctggaattttagtgctttgcgcactgatatgtgcattgagattgggccccaaatgggtt
7981tctgaggggatgcaaccccagaaagtttgctctgataggactggggaaggtttactgctc
8041attctgacattgtaacccttgtcatttccataattttaaggctatataagtcagccaggt
8101tcattaatttgaaaggagtttctcccttgtagttaggatgttaagttattttttcaaggg
8161cccaagattgggctgggatcaggattggtagtctgtttggatgatgatggagataaacaa
8221agccaacagtgccttgccaaagctggactggtggtatttaacagtctttgtgttaaagtt
8281tttaataaatacccagaatccattaattaccagaggggtaaagcgaaactcttttgtaaa
8341ataaagctgattatcagtatgcatggtcttagtatacacaggctgtgggtgaccattatg
8401gtataaaaaaaaaatacttatttgttattttgttctatagataaaaattaattaaaacac
8461tttaaaagaagtgattcaatccatttggaagaaagcaattaaattgtaaaaatataaaaa
8521tggctactattatatccagcttacagtaaatatacaagaaggacaccaaggaaagttgat
8581aggaatttacttatcttttggctgccttttaaacaggtacttcaggtcttctacaggttc
8641acaggtttagtggctgatgggatcttcaggttccaggtctgggggcttcagttattgcag
8701gctggaccctggaaagacgtatccaactaactattcccagtactttgactggagaaggca
8761tggccagtaccactgaaaatagtcccttccatttgggttgtaattgttcagcaggtgatc
8821cctccttccatgttttaacaagtactttatctcctggcctgattttgggttgctggttag
8881ttcctggtgtggggagtctttgagttctaaacttttgtaaagcctgctgaaattgtccca
8941ggttaattacgcatgttactaaactggctgtttctggatcagtaattagatcattattta
9001aaaatggccagccaggagcggtggttcatgcctgtaatcccagcattttgggaggttgag
9061gcaggcgaatcacctgaggtcaggaggtcaagaccagcctggccaacatgatgaaaccct
9121gtctctactaaaaatacaaaaactagtcaggcatggtggcacatgcctgtaatcccagct
9181actcaggaggctgaggcaggagaattgcttgaaccagggaggcagaggttgcagtgagcc
9241aagactgtgccattgcactccagcctgggtgacaagagcaaaactccatctcaaaaaaaa
9301atggccttctgtataacatttcatatgagctcatattaatttttgctctaggagaattaa
9361gggttcttaagagggctacaggcaataagctgacccaaatttctgatgttttctaacata
9421gcttagctaacacccattttaagagcttgattagccctttctactttcccatgtaaataa
9481tttttgattctgagagccttagcagccccttgagttctaaaagatgggtctttatcactt
9541tagaggctttggggtaacccaaactaggggatttatttcctttaaaagaagcttagcaac
9601ttcatgagccttctttgttctcatagagcaagcttcaacccagccagtaaaagtatctat
9661tactactaccaaaaacttgtatactttgcacactggcatatgggtgaagtctaattgcca
9721gtcttcccctttaactttagccaatatgttcacacacagaatctcttttacaattaattt
9781ttcataagccttccacaacttgttcaaaccttttagattattcctatctcacttaaaacc
9841atcctttcaccctctaaacttaggcaagaaatccacattcccatgctttttaccaaaggt
9901acattatccttttcttacatgaattcttttcataacttacccagaccatctacaaaatgc
9961ttggactttctgagttttcctaaacatctttctttttaaacaaccagtcattttacgtta
10021ggacaagaatttatcgtacaagatcttttctcctacaaagccaccatctctacaatcttc
10081cctcacaaaaacatatcttcatatatataactttcttcacatctctcccctacttactgg
10141ttccttactaccttgtttcataaataactcttttttttaaagtccataatttgaattaac
10201tttcagataacctccaaactacacaaaataattctttctcaccaataacacattttttgg
10261catattttgtatacagaattatatattaactataattcatatccttatgtattagtctgt
10321tttcatgctgctgataaagacatacttaagactgggcaatttacaaaagaaagaggttta
10381ttggacttacatttccacatgctgggaaggcctcacaatcatggtggaaggcaaggaaga
10441gcaaatcacatcttatgcggatggcagcaggcaaaaggagagcttgtgcagggcaactcc
10501cattttcaaaaccatcagatctcatgagacccattcgctatcatgagaacagcataattc
10561aatcatctctcacccactggatccctccccacaacacacggtaattatgggagttataag
10621aagagatttgggtggggatgcaaagccaaaccatatcatcttagtaaccttaaattttag
10681tgaaaccttaggaagcaaaaaatcctaaactatcagatattagcattttatagatgagaa
10741taattccacaatttttggaaacatattcctccctatcataaccctttcttaattggaaat
10801gactgagatatccaatgggcatcaaaaatacttttaagattttaaattacacaaaattta
10861cctaatcattcatcccatttacatgtacttaagtctttcatttttaacagtttatctaga
10921ttacttctgaaaactgagatattagacaccatcatttaaagttagttatttctttgttaa
10981ccatttttaatagccaatgaacattatgtgttcacctaaataagaacctcacagttaaat
11041acataggtgtttttgccaataacccagaagatttagctaacaatattaaattagtctcat
11101ttgtcaaagaagccacataaactaaggccattttgttttggctgggttaatagctttata
11161atcttctatgccaaatgctgacacttcaacatttctagcaaagacaaatataaaacccag
11221acaaaaatgtatgctgacaattctgaagatatttctattttcattttaccaataatttta
11281aagccagctatttaatgaagatttacttaagtcacatgaacttgaaaattgcttggactt
11341atttatttaatttatgagtgcttttttatttataagctgatttggttgacacaacatata
11401acaataagtgtacatacaaataaacacatctagacatgtatacacacacacaaacgaaga
11461tccaatagcttttaccttggaactctagccatgagatatcaatacaagctcacaggtttt
11521acatggttacactttgtttgctccaataggtaatccagtgaaggctgtgaaccaaacatt
11581tttaaagcagttccggtggcagtttgatttttaaaggccaaacctcctcagactccaaag
11641acactggagccaaatagcaccaaaggagagcaccatatgctgaccaggcctgacccttct
11701taggacagcagcaccaaagcctggatacatgcaacgtcatcccgctttcccattcaacag
11761caaactccagattccaaacaatactggggccaagtattattgcaaaagaatatcaaatgt
11821ttcctccttagattatcagggtcaaattcatttcaatggttgaggatgcagccaagtggg
11881aagtcaggtggaataaatggagtgtttcctgtagtggtctgcaagagagagacaattcta
11941aggaggccttagtactagacctgagaacctttgcctagggcatcccctttggagaggctg
12001aggtctggaggtggatcccctaggcatccccctttgaggtccagtctaaaagtgtcagac
12061atctctgaccttaggtgggcaccagtgttgctttgtatgttttccctccagaggcaatgg
12121cctaatatgagcttttctcttgtccctggatgaaggtctcagcttctagcttcctaccaa
12181tttagataggccatatcttcctgcattccttgcaagactaaggaggttcccgaacactgc
12241agagttaacggtaccaaggctagtgggatattccccatgaagaaatggtgcagaggttat
12301ataaaagattgggtttatattgggtatataaaagatatatagaatttctacggggttcag
12361ggcaagcaaactgatgccaaagcaatgaccaccaagctcccttaagtggaggtccaagat
12421gtagaatgtgttctaaagcttgtgggtcccaaaggtcctcagccagtcaatagattcagt
12481aggagaagggaggtcaaaatatctaagagatattatcttttgtccttacaggccaacttt
12541atgggagaatttagcttaagaaagagggtttaagtcgcctgaaatgtgtgtgagttcacc
12601ctggacgagcttctgctgccaattgcatcacaggtagggatcagggactataactcaaaa
12661agatacaaaagagtccttccgccttctgggcaggggagctatcccaggttcactccttgg
12721ccttcaggtaacagtagagagtggtcccggccagtttccctcaattaccaagaagctact
12781aggaaacggccactgaaagactgaaaaaaggaaaaggactcaggtccctcacctgaactg
12841ggcagtggtggtcaggcactttcacacagaaacctttcagtttcaccagagagtggccct
12901gttagaaacctgtggttgcctctgtgcttaggcactgtccaccgagggttccaagctgga
12961aagggaaaaagagacagagaaaagagttcccctgtaaggagcagagaggaaaagggaaat
13021caagagaagaaaaataaatcccaaactttgggcttacctcctggctggctcgccaatata
13081tgttactggtggagggtcttgactatgagtcatccaggttcttggcattttgaacaagga
13141actggacaaaatgcacaaaccaagcaatgaaagaatgaagcaaagaacgcacagatttat
13201ttaaatgaaagtacactccacagactgggattgggctccagcaaggagctcaagaggccg
13261gttacagaatttcctggagtttaaataccctctagaagttttccattggttacttggtta
13321taccctatgtaagtgaaggagtggcccacgacaagtctgattggttgtgggaggggacca
13381atcagaggtactttccatttttatctgtgaggcagtgcaaagacagtagcctctgatact
13441tttgttacttgggtgtggagaggtggggttttccttgtgattcagttctaggaagtcagg
13501gcaaattggccttagattccctgcctccagaccctattcttctacctcacaagcatgtca
13561tgaatggtctggagtaaatcataagcggatttatggaaaaaaaagcttttagataatcaa
13621gttgtctataattaaagggaaattatttttaatggtctttctaggaattgggttttggta
13681ttttctaaaaaccatttatatactgaagaatcttagcactttgggaggccaaggcaggtg
13741gctcacatggtcacaggttcaagaccagcctggccaagatggtgaaaccccgtctctact
13801aaaaatacaaaaattagccaggcgtggtggcagacgcctgtaatcccagctactcgggag
13861gctgaggcagagagttgcttgaacccaggaggcggagtttgcagtgagctgagattgtac
13921cactgcactccagcttgggtgacagagtgagactctgtcacacacacaaaaaaaaaaaaa
13981aaaaattggttagaacaatggaattttcttgaagtattgcttcattcttaataaaattaa
14041aattgataaaagacattataattttttaagtaaatttcaacttgtattgcatctcactgt
14101tttcagctttttctccccttttaaagtgcctgaaataatcactctattcttcaactcatt
14161ttcagcttctgtaagcttcttttttttcgggttctgttgtggtctgatactaaaaatgtt
14221ttctcttaaaggtctaaaggaaatgtttccttccaacataatattctgtgcttttggctt
14281taaattgttctatgaaataaaaaattttcacttacgacccaggaaacactcttcctatgt
14341ctaactgtgtccagaattggttcctgcctatgggttcttggtctcactgacttcaagaat
14401gaagccgcagaccctcgtggtgagtgttacaattcttaaagatggtatgtctggagtttg
14461ttccttcagatgttcagatgtgtccagagtttcttacttctggtgggttcgtggtcttgc
14521tgacttcaggagtgaagccgcagacctttgcaatgagtgttacagctcataaaggtagtg
14581cagacccaaagcgtgagcagcagcaagatttattgtgaagagcaaaagaacaaagcttcc
14641acagtgtggaagaggacctcagccagttgcagctgctggtttgggtggcgagcttttact
14701cccttatttggccctgcccatgccctgctgatcggtccattctacagagcgctgattggt
14761ccactttacagagtgctgattggtccatttttagagtgctgattggtgcatttacaaacc
14821tttagctagacacagagcgctgattggtgcgtttttacagagtgctgattggtctgttta
14881caaacctttagctagacgcagagcactgattggtgtgtttttactagtgctgattgttgt
14941gcttacaaacttttagctagacacagagcgctgattggtgcgtttttacagagtgctgat
15001tggtgtgtttacaaacctttagctagatgcagagcactgattggtgtgtttttacgagtg
15061ctgattgttgtgcttacaaacttttagctagacacagagcgctgattggtgcgtttttac
15121agagtgctgattggtgcatttacaaacctttaggtagacacagagcgctgattggtgtgt
15181ttacaatcctttagctagacagaaaagttctccaagtccccacccgacccagaagcccag
15241ctggcttcacctctcataactaattcaagtatctctttaattagttttgacttgcagtta
15301atctaaatggattctccacagggaacagcagtcgcagtgcagaagctctttgcttttgct
15361tttgggtaactggcctaacaaacagattttacactttatattgaaataattcctatgtca
15421ttattactaagttttggtctgcttagaaaaaactgagattaaaagaaaaactatttttca
15481ttaaggttattacatctgtgtaactttctgtatgtgcttttaaagtccttgtgccatcaa
15541gttacagggtttagactcctgggtctaaaaaggatgccaagtccggctaaatcttaaaca
15601ctgacagcagttaaagctttatcttcagaccctgtagaagataccaatcaaaataaactg
15661tgttcatgagacacagggccagaaattaaaactattcaactcctcaagacccaggaacta
15721tcatggaagacatgggtgtgtgacattgtaagggtcaattttgagagataaaataagttt
15781agtttctctataaattaaccattaatgtcgaaggcactgatgcaagactagcatatgggc
15841ctctgagtcagattaacaaggttttcttggagcgttaacccactccttaataaaaggtta
15901taaaaaaggcttatggaaattacatcttatggtcaagatgattaaactatttaatattat
15961aggctgtttataaaattttggagaacaagtttaattggcctcatcctgtctttattaagg
16021cttattgtttgggaaatgaagtctcctctcaaagaataaaggttttcgccttaaaaaaaa
16081tctttgagttatcgctttgtctaaatgaatgactttacaatgacctgtgatcctactttg
16141tgatattaagtgttttaaactttttatgtttgacaaaccttcgacaatcaaattctaact
16201ttggtcctcattaattttttgatattagtcccctgaagtccaaaagagacatatttggtt
16261tatttgatataataaaatcatacaggaagtattgtcaagtataaaacaatgtttaatctt
16321ctttggattatatttatgcacatatgttattggtatatgtttgagaattgtatgaaattc
16381ctgtgattctgatatgacttggcatatgttatcagtggtaattattattattatgtaatt
16441gtggtaattataattatgtaaaattgttgtatgccacagaagtaaccaaatatctttgtc
16501aattgtgtctttatgactgttctaagacttttgttatccacagttgttttacttttatcc
16561tattcaaaaggtggttttataatcagctgtgggactctgacaggtgctcttgaatgcagg
16621cttctaataactttggagactgtgacactagaatagaggaaaaacttccaagactccagt
16681ggagagctgaaatgttcatgaatatcaagcagaaaagaagttaactgcatggactgaact
16741aatagaaaactgaaatagtccttttatgactttttgcctaaaacattgctgaccctttgt
16801ttttcagagccaagaaaacttttcttttgagctatttatagcttttaacaatggagtaaa
16861gtatatttctgtaaacaaaatttggagcatatttctctctacctgatttctccaaaattt
16921agaaactagttgtgagtattcttaacttatggcaatatagttatttgcatacatgcaata
16981agaatctttcctctgagaaagattctttctcaagaatgttcctttgtaacagggcacaat
17041tggagacactggttattttaccaaggttttgactggaataggatgctttcagatacaaac
17101agactcctttaaagaatgaaagttgactttagagccaataaaaactccttgagaaacctg
17161gtctcataccttgtctgcacagtcccggaacagggtgacctgtagtgagtaaagaatgtc
17221actttctgacaggcccaggagccccaagttttcttgggaccttgaagtgaggaattcact
17281caactaacgcaaggatttgcaggcacaggccaggcttaatgcattaaagttaaatctgag
17341attcttagtggaataaagttctagcaaagccaattaaaaaaaaagaggagcctatatagc
17401aaataattattcttctgactttatgcaaatactccagccaagtataataagactacaact
17461tattttgcaaatgaatttgtcctatgacttgtctttagtgaaaatgagactggagagagg
17521aaaattatatttcaacataaactatagtacacctgttattagattctagtcttgcctaat
17581gtttttcagttttttttattttctacaatttagattgaattcaaaaatttttcctggcta
17641caagtctccaaaataatgttttcaatttttttcttctttcgtttacttccccccacccca
17701tttttcctgatttgaaatcacttaaaattaagcagtctttcttaaagccctacaaactga
17761agctagacaatgtaaacttcagaagaaaataatagcaacctatttacacacataaaccac
17821tttcatacctgcctactgacatggacttcagagtaatacggcctatattgattttccagg
17881attcttcttcattttgtttgtttgttgttgtttttcttccttcctccccctattttctct
17941tcaaaggacatgagacttcataacctactaaaaatgagctttccaaataacgtggtactt
18001atccatctaggaataaactatcctagccatgagagatcagacaaagcctgagaccagaga
18061cttgttttcttctaaaatgctgtctctgaaagattttaaaaagaaaaacggggagatgtg
18121agaggaaaataaatctcaggactccaaaattactaagccaaagggaaaagccaagctggg
18181aactgtggggcaaacctgcgtcccattgtactcctaaacaagatagcaacaaagataaaa
18241aagctacatacctccctcacaatttgcccacaagaaaattccctgtagacaaaagacaga
18301cagaactcaaagtcatccttttgctcatgtgagataaatgcatatctaattgcttccttt
18361tccctattgtttcactaagccagactaaggcataagtgactattcctgtaagttatgtat
18421tcagtgaaaggttaattagaaactcaaaagaatgcagtcgtacctatgacctggaaaccc
18481aggtcataggtatgtatcagtgaaaggctaattagaaactcaaaagaatgcaaatgtacc
18541tgtcaccagggagccccctccctgcttcatgttgtcccatctttccagacgaaactgatg
18601tacatcttacatatattgattgatgtctcatatctccctaaaatgtataaaaccaagttg
18661tgccccaaccaccttggacacatgttgtcacgttgtcaagacctcctgaagctgtcttgg
18721atacatgcttaatcttagcaaaattaactctctaaattgactgagacctatctcaggtat
18781tttgggttcagataaagttttagatatggactcactgtaaaagcaaggcttgttgtttct
18841aagaactatactgcggagatgaaaagagggggactttcaggctggaaactatcacatcca
18901cattcctttgcacatcagcagcagagtctttctttatacatggacacaatccaggctcct
18961ccctgagaactgccttgcagtcagcaggagatgaagggtgttaggacacttagaactgag
19021aagagttggccgggcacggtggctcatggctgtaatcacagcacttcgggaagccaagac
19081gggcagatcactgaagtcaggatttcaagaccagcctggccaacaccctgtctctattaa
19141aaaatataaaaaaattacctgtgcatggtggtgtgtgccagtaatcccagctactcagga
19201ggcagaggcaagaaaatcgcttgaacccaggaggtggaggttgcagcgagctgagattgc
19261gccactgtacgccagcctggcagcctgcgtgacagagcgggactccgtctcaaaaaaaaa
19321aaaaagagaagagtttatgatataatgcaagaagggaggggcagaccttaaagacataaa
19381cttactacgtttcaacaccatacatttgaaaggggccacaggactggcccagaaagtact
19441ccatatgtataaaagggctgcagtttgtccggtggtatagaacattaagaatctatttgt
19501ttgttgagacttcagctttctcttactccaaacatgctgtgctgttttgtcatagagagc
19561tgatgtcttcagtagcagataccccaagtctcttcatgcctcttctttatccctatcaat
19621tctctctctttacttccttccccttccaatctagaagcctatattagtcagctcaggctg
19681ccataataaaataccatagactgggtggcttacgcaatagaaacgtacttattcacagtt
19741ctagagactagaaatccaaaatcagagtgccagcatggtcaggttctggcatgggccctc
19801ttcctagcttgcagatggctgccttctcactgtatcctcacacgacagaaagagagagca
19861aacttcctgatgtcttataggagcactaatcccagcatgagggctctattttgtaacttc
19921atctaaacctaattacctcccaaaaggccccatctccaactactgatacattgggggtta
19981gggcttcaacatatatattttggggggcacaattagcccaaaatatggcccaagtctatc
20041attttggtcattctcttgctggtggtcttactctcttgcccttttgtcctcctggcgcaa
20101ccttttagtcaaaccccagtctgggattaggccagtagtccctttccatggggcagctcc
20161cagctgttgagcactgccagggaaaagcacagcagattaaggacctgtggccactgaaaa
20221ttagggtctcctcagattcatgtactgatatgaaagaactatcacttatacatgggtctt
20281tagttcaaaaaacagcaagatgcatttcagtaggcttaatacaatgatttctgtagaaat
20341gtaatgtgttatctatgtgaaatggacttgtattagcttagaaatagtacaagttggctg
20401ggcgcgctggctcacacctgtaatcccagcactttgggaggccgaggcaggcagatcacc
20461tgaggttgggagatcgagaccagcctgaccaacatggagaaaccccgtctctactaaaaa
20521tacatggtatttttgtaggcatggtggcaggtgcctgtaattccagctactagtgatgct
20581gaggcaggagaatcacttgaacccgggagggggaggttgtggtgagctgagattgtgcca
20641ttgcactccagcctgggcaacaagagcaaaactccgtctcaaaaaaaaaaaaaaaaaaaa
20701agccaaaagtttggtcactcagtgtcaagctggaacacaggagtcctttcaaaggttcag
20761aatcccatcgctaggacccagtctccgccagagcaacacaagaccatttctccctgagac
20821cctggcactgtcacagtcagtgaacacatctccatttattctggcatccacgggccttgg
20881ttctgcaggctttcacctcagagaaaatcttgtcactctggccacttctgcttctcactc
20941tccaagcccctgaatgcctgcccccgacccaaccctactttaacagattagaagataaag
21001ttttcccttatctatgccctgggaatcaaggagggagggaagagaaacaaacctcttccc
21061tcctgatgcttctgtaccagcaaaatcaaaagagaattaaatcttacaacaaattgcact
21121gcttcagctattgacactgtcccacagaattcgagggacctcacctcctcgtttttggac
21181gacagaggctgaaacaaatctttgctaagggaggctggaagttggggcaaaacattatta
21241atgggtcacaggccagaaaggagagtcaatgagagaggattaatgaggccagatcatctg
21301gtccacaggctgggctgactcatcctgcattctgaggcttgatcttcagtgcacctacct
21361gtgttggctcctgctagggctgcgtgagcctaaggaaataagactaaaaaaatcaagcct
21421tgtgtatgagtctgttttcatgctgctaataaagacatacccaggagtgggcaatttaca
21481aaagaaagaggtttaattggacttacagttccacgtggctggggaagcctcacaattatg
21541gcggaaggcaaggaggagcaagtcacatcttacatggatggcagcaggcaaagagagctt
21601gtgcaggaaaactcccccttataataaccatcatatctcgtgagacttattcactatcat
21661gagaacaacacaggaaagacctggccccatgattcaattacctcccactgggtcccttcc
21721acaacatattggaattcaagatgagatttgggtgaggacacagccaaaccatatcacttt
21781ggaactctcagcttctcaaggggtaaatgttgattaaatgactatatgacaggtaccagg
21841gctcttatatggcacagaagattcagggaggatactacctcactgctgtgacactcactg
21901tcaaagccccaagaggcaccttgaagggacttcacttctcaagaaaaggaattgtatact
21961cagttcagggaagattcctgtattagttatctactgttatgtaacaaattactctaaaac
22021tagcagcttaaaaaacaacaaatatttattgtctcaatgtttttgaaagtcaaaaatatg
22081gaagaagtttagctggcaagttctggatcagggtctttcataaggttagatttgagatgt
22141catctggggctgcagtcatctgaaggctggactggggctgaaaaatctacctcctgcata
22201gctcaaccccatggctgttggcaggaggccacaggtcctcactgactattggcactaatc
22261ataaaaaagttggagtggctatattaatgttaggctaaatatattataagataaaaagta
22321tattttgagatgaagaagaatatttcataataaaaaaaatgccatttgccagtaagatat
22381aacaattataaatgtgtacatgcctcataacaaattcaaaatacataaagcaaacgtgaa
22441agaactaaagggagaaatagacaaatccacagtcgtggttagatactttaacttcttctc
22501ttagttactgatagaactagataaaaaattagtaatgatcatgaaagttgtcagaatcta
22561agtggaattacttatgtcaaactctaacaaaaataaataaataaggctgggaggctgaga
22621agcagggcggtgtgtgggggggtgttgcacacatatctatgataagaacacgattacaaa
22681gactctctgaagagctcttacccacccatgcctgtaagaagaacttttgtcaaagacttc
22741ccaaactgctacatgaatcacaatgatagctagccagatgtacaacaacacctgcctgac
22801acactgtcttcactaatgaactggcttcaactcctgtgataagcccctgtaaccaatatt
22861ctctgtttcaaggcaaattatgtgtccttctccctgttgcctttaaaagcttccccttgc
22921ctcagcctcttcagttatgcctatggtcccaagagccctatatcctggatttgcaagtcc
22981cctgcacatctcaaataaactcaatatctctggagaatctccctctgtctgttatttggg
23041ttgaagtaatacagaagatctgaacaacactatcaaccaccttggtctaattgacatgta
23101taaaacactacatacgataactggataatacacaatttttaagtacacatggagtgttcg
23161ccaagatataccatatgttgagtcataaacaagtcaataaatttcaaaaggctttctcag
23221atcacacaggaattaaactagaaagcaacaattaaaataataggatatgttaaaaagccc
23281taaatatttggaaattaaataacacttttctcgacaaggagtaagtcaaagaagaaatca
23341taaagaaaattagaaagtactttgaactaaatgaaaatgaaaacaatgtatcaaaaattt
23401gtggatttaactaaagcagtaagtagaaggaaatctataatttcaaatgtttgtcttaga
23461agaaaggcttataaattttcatattgagaagctaaaaaagaagagcaaattaaacccaga
23521gtaagtaaaaaggaaaacattataaagataaaagtggaaatcaatggaatagaaaataga
23581caaaataatagagaacattaacaaacccaaaacttttatgtttaacaaaattgataaaat
23641tggtaagcttttagcaagactaatcaagaaaagagagggagagaaaaaaactaattactg
23701gtatcaggaatgaaagaggagatttcattccagatcttatacatatattcaaataatact
23761actggaatattatgaacaactttaaaacaataaatctgataacttatatgaaatggccag
23821attccctgaaaaacaaaacttaccaaaagtggcaaaagatgaaatagaaagcatggatgg
23881ctctgcaactgcaaaaaaacttttccacaaagaaaactccagaattcactggtgaattct
23941atcaaatggttatggaagaaatgataacaatcttacaaatattgaaatttcagaaaatag
24001agaagaaagaaactctgattttataaagccaggccaaaacctggcaaagatacaagaaaa
24061gaaaacaatacactgattgcctgtatgttcatttttcatgaacatacaggcaaaaatctt
24121tatcaagatgttagtaaataaaatccagcagaatataagaaagacaaaaccaagttgaat
24181ttgttagcattacactaaatggagaaacattgaacacttttccctctaatatcaggaaca
24241aggcaagatgttcattcactgcttctattcaatattgtgcaagatgacctagactacgca
24301accacgcaagtaaaagaaaaaggcataaatattggaaagaaagaagtaaaactgattcta
24361tttacaaagtatatgcatattaatacagaaaatcctaaggaatcggccgggcgcggtggc
24421tcacgcctgtaatcccagcactttgggaggccgaggcgggtggatcatgaggtcaggaga
24481tcgagaccatcctggctaacaaggtgaaaccccgtctctactaaaaatacaaaaaattag
24541ccgggcgcggtggcgggcgcctgtagtcccagctactcgggaggctgaggcaggagaatg
24601gcgtgaacccgggaagcggagcttgcagtgagccgagattgcgccactgcagtccgcagt
24661ccggcctgggcgacagagcgagactccgtctcaaaaaaaaaaaaaaaaaaaaaaaaaatc
24721ctaaggaatctacaaccccccgaatctacaacccccgcccctacaaaacaaataatagca
24781ctaataagtgaaagtctcaatgtcactatacaaaatcaattatatttctctacattaaca
24841gcaaatcaatggaaaataaaataacattcacaagactcaaagaataaaatacttaggaat
24901atatttatcaaaatgcattcagtgctttaaactatgaattctccccaaattaaactacag
24961gttcacaattccagcaggtatttttacaaagttgataagtcaattctaaaatttatatag
25021aaatagaaagaacctggaatagaaaaacaattttgaaaaagaagaaagggtagacaatat
25081acattatttgacttcaggaattatcctcaagcaatagtcatcaaggaagtgtgatactag
25141cataaagattgatagataaatggatcattagaacagaatagagaccccagatataggtcc
25201accttatatggtgaacttatttttgacaaaggcactagagctgatcaatggcagtaattt
25261tttgtaagccctacttttttccaacaaatgatgctgtagcaactggatatctatatggga
25321agaatgaactttgactcttacctaaaactatacacaaaagttaattcaagatgcatcata
25381gacctaaacataaaactactaaaattttggacaaaaatatgggtgaatatctgagagtag
25441gcaaagattttttagaacccagaaaaaaaacaaattgataaattagacttcatcaaaatt
25501aaaaatttctgcttatcaaaaccatcattaacaaacaggcaagccacagactggaagaaa
25561atatttatgaaacatgtacctagcaaaggactgtatccagcatatctaaatctcctataa
25621ttcaatattaaaaagcaaagacaaaaaatgtggatgtttcacaaaagaagaggtacaaat
25681gaccactaagcacatgaaaacacaatcaactctattaatcaccaggaaaatgcatacaag
25741tcacaatgagatacaactattagaatagctaaaatctggaaggccggcaacatcaaatgt
25801taacaaggatgtggagcaactagaactcttatatattgctcttaagaacataaaataata
25861cgaccacattggaaaaacttgtgacaatctaataaaattaaatatgcatctattgtttga
25921ctcagtaattccgcacctagatatttacccaagagaaatgaaaacatatagctacaaaaa
25981aagatgtatgagaatgttcacagcagcattattcataatggtctacaatatgaaacaacc
26041caaatgtctatcaactagtgaataaattaataaattatagtatattcacacaatgggata
26101cttcagagcaaaaaagggcaagtttctgatatgtgaaacaatatggattaatctctcaca
26161cattatggtcaattaaagacatcagacacagagtacatattacattatttcatttataca
26221acagtctagatctaagaggaaaataaatgagaatagtgatcgcctggttgtggagggtgg
26281gatgaccgggatgacatgcaagggaactttgtggagtcaggggaaaatgttctttatctt
26341gcttggtgttggggttacaaaaggatattgctttgtcgaaacccattgaactatacattt
26401ttgttcattttaatgtatgcaaaatttatttccctcaaagaaatgaactttaataaagca
26461agaaaaatatatgatcatacatacacaatactagggcctcttgcagggcacttaaagagc
26521ccaacagagaaaggggctaaactcacttgaagcttcattagctatgtagaaaatctgcct
26581ctgcccccagctcacattctggcccaacactcacttagttgtgtgaccatggacaagctg
26641tttgatatctcagtcccttgaatcccacatgtgaaaattctgaataactatagaacctat
26701ctcactaaattggtgaataaattccatgagataaattagaactaactggtacatagtgag
26761tgctcaacaaacataaactattatcattattgtgcagacacagttttcaaaagaaaactg
26821tttgggaaattcttccaagggccatgaagagcctactgaagtgtcctgagaagagaaggg
26881acacagtcagatttgcctttcagaaataatgactgggccgagtgcagtggctcacacctg
26941taatcccagcactttggaaggctgaggcgggcggatcacgaggtcaggagttcgagacca
27001gcatgaccaacatggtgaaacctcgtctctactaaaaataaaaaaattagctgggtgtgg
27061tggcgcacgcctgtaatcccagctactcaggaggctgaggcaggagaatcgcctgaaccc
27121gggaggcggaggttgcagtgagccaagatcacgccactgcactccagcctgggtgaaaga
27181gcgagactccgtctggaaaaaaaaagaaaaagaaaaaagaaagaaagaatgactgaagca
27241gcagtggggaggatgaattggtcggggaggagggaagaacggatttggagtcctttcagg
27301actgtcctaacatgaaaatggatgagacattattgatctaaggaattttttagaaataaa
27361atagttttattcaaaggatatagaatttcaacattttaataaaaatctaaataaaaataa
27421aagtaaaacttaaaatgatttttcacatttaaagatattaaaataaattcaaaattgcct
27481agtaaaataaaagccaaataaattataattaatatcaaatcttgaaataaaagtgtttaa
27541aaatataattgttcaatattgcagaaggttcttaagcaacccaccctgctcatgttgaat
27601tgtaagtacttctccagaactgcacactggaacgggaagagaaataacatggactttcac
27661accattaggaaaggagacttcattatactgagcccgtctactcctgcacactcctggggt
27721gggattgtgggtcacagggagacgtacgtgcatactgccgaagagctcccaagtggctgg
27781ctgcaccagttaacactcccctgagcagtgtaggggagtctcagagggtcacttggcatt
27841ttccatcattttcattttagccatttttgcaggtgtgcgcaatgctgtttttataaccaa
27901cacatcatttttctcttcctttcctggagggggaacccagcttttctaggccctccaccc
27961tttgggcaggaggaactgggtgtgtggggcggggggaagccatctctgggtggttggtca
28021gctggggcctaaccacaggaggcagatagactgaggctatgccaagcagaaagacttatc
28081aggcacctcttgcctttcctcatctctgccggttcaaatcctgctcgccccgcaagggca
28141gattggaatttaccttctccagaaagcctggggtgactctatcccctcactcctcctccc
28201ccgttcgccagcttttgtcttgacatcggcggaccaagaatgcgtgactctgggcaaatc
28261attaacctctctgggcctatttccttatctggggaacatctgcacttgagcaacagaagt
28321ctaagatccctatctgatttgatgttttgcgacccttttaaggcgca
<210>2
<211>20
<212>DNA
<213>artificial sequence
<400>2
cccattggagaggactgtgg
<210>3
<211>22
<212>DNA
<213>artificial sequence
Claims (10)
1. the long-chain non-coding RNA ENST00000581794.1 of a high expressed in patients of acute myocardial infarction blood, it is characterised in that the sequence of long-chain non-coding RNA ENST00000581794.1 is such as shown in SEQIDNO:1.
2. the long-chain non-coding RNA ENST00000581794.1 of high expressed in patients of acute myocardial infarction blood according to claim 1, it is characterised in that this long-chain non-coding lncRNAENST00000581794.1 is the total length of ENST00000581794:1.
3. the preparation of long-chain non-coding RNA ENST00000581794.1 according to claim 1 and 2 or diagnostic agent or medicine or test kit in a vitro detection blood, it is characterized in that said preparation or diagnostic agent or medicine or test kit include specific primer to, standard DNA template, PCR reactant liquor, wherein, specific primer is to including forward primer and downstream primer, the nucleotides sequence of forward primer is classified as SEQIDNo:2, and the nucleotides sequence of downstream primer is classified as SEQIDNo:3.
4. the preparation of long-chain non-coding RNA ENST00000581794.1 according to claim 3 or diagnostic agent or medicine or test kit, it is characterised in that this test kit is fluorescent quantificationally PCR detecting kit.
5. the preparation of the long-chain non-coding RNA ENST00000581794.1 according to claim 3 or 4 or diagnostic agent or medicine or test kit, it is characterised in that specific primer to for SYBRGreen, Taqman probe, molecular beacon, double cross probe, combined probe detection.
6. the preparation of the long-chain non-coding RNA ENST00000581794.1 according to claim 3 or 4 or 5 or diagnostic agent or medicine or test kit, it is characterised in that PCR reactant liquor is fluorescence quantitative PCR reaction solution.
7. the preparation of long-chain non-coding RNA ENST00000581794.1 according to claim 6 or diagnostic agent or medicine or test kit, it is characterised in that fluorescence quantitative PCR reaction solution includes dNTP, Mg2+, Taq enzyme.
8. the preparation of the long-chain non-coding RNA ENST00000581794.1 according to claim 3 or 4 or 5 or 6 or 7 or diagnostic agent or medicine or test kit, it is characterised in that also include fluorescent dye.
9. one kind detect long-chain non-coding RNA ENST00000581794.1 according to claim 1 and 2 method, it is characterised in that carry out in the steps below: the first step, extract blood total serum IgE；Second step, prepares cDNA；3rd step, quantitative amplification lncRNAENST00000581794.1.
10. a long-chain non-coding RNA ENST00000581794.1 according to claim 1 and 2 is used for detecting the test kit of acute myocardial infarction or being used as to detect the application of the novel targets of acute myocardial infarction medicine in preparation.
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