CN105801881A - Hyaluronic acid silica gel and preparation method thereof - Google Patents
Hyaluronic acid silica gel and preparation method thereof Download PDFInfo
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- CN105801881A CN105801881A CN201610214952.2A CN201610214952A CN105801881A CN 105801881 A CN105801881 A CN 105801881A CN 201610214952 A CN201610214952 A CN 201610214952A CN 105801881 A CN105801881 A CN 105801881A
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- China
- Prior art keywords
- molecular weight
- hyaluronate sodium
- hyaluronic acid
- weight ranges
- sodium
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 title claims abstract description 76
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 31
- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 31
- 229960003160 hyaluronic acid Drugs 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000000741 silica gel Substances 0.000 title abstract 5
- 229910002027 silica gel Inorganic materials 0.000 title abstract 5
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 74
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 68
- -1 polydimethylsiloxane Polymers 0.000 claims abstract description 50
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 32
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 23
- 239000008213 purified water Substances 0.000 claims abstract description 22
- 239000000377 silicon dioxide Substances 0.000 claims description 37
- 239000000017 hydrogel Substances 0.000 claims description 34
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims description 32
- 239000012071 phase Substances 0.000 claims description 20
- 239000008346 aqueous phase Substances 0.000 claims description 18
- 230000001804 emulsifying effect Effects 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 11
- 229920002521 macromolecule Polymers 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 8
- 238000013019 agitation Methods 0.000 claims description 7
- 229930182478 glucoside Natural products 0.000 claims description 7
- 150000008131 glucosides Chemical class 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- 239000000499 gel Substances 0.000 claims description 5
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical compound [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 claims description 4
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 1
- MJEMIOXXNCZZFK-UHFFFAOYSA-N ethylone Chemical compound CCNC(C)C(=O)C1=CC=C2OCOC2=C1 MJEMIOXXNCZZFK-UHFFFAOYSA-N 0.000 claims 1
- 231100000241 scar Toxicity 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 8
- 208000032544 Cicatrix Diseases 0.000 abstract description 5
- 230000037387 scars Effects 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
- 210000002950 fibroblast Anatomy 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 abstract 1
- 235000011187 glycerol Nutrition 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 229940010747 sodium hyaluronate Drugs 0.000 abstract 1
- 210000000589 cicatrix Anatomy 0.000 description 25
- 239000003921 oil Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 12
- 238000005303 weighing Methods 0.000 description 8
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 7
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 7
- 229960002591 hydroxyproline Drugs 0.000 description 7
- 230000008439 repair process Effects 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 7
- 240000007711 Peperomia pellucida Species 0.000 description 6
- 210000000835 hypertrophic cicatrix Anatomy 0.000 description 6
- 208000003251 Pruritus Diseases 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 230000002980 postoperative effect Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 206010052428 Wound Diseases 0.000 description 4
- 230000036407 pain Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- YZUUTMGDONTGTN-UHFFFAOYSA-N nonaethylene glycol Chemical compound OCCOCCOCCOCCOCCOCCOCCOCCOCCO YZUUTMGDONTGTN-UHFFFAOYSA-N 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 206010040882 skin lesion Diseases 0.000 description 3
- 231100000444 skin lesion Toxicity 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 231100001274 therapeutic index Toxicity 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 206010065952 Hyperpathia Diseases 0.000 description 2
- 206010062575 Muscle contracture Diseases 0.000 description 2
- 206010053615 Thermal burn Diseases 0.000 description 2
- 208000006111 contracture Diseases 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 208000035126 Facies Diseases 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010039580 Scar Diseases 0.000 description 1
- 206010043189 Telangiectasia Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000002316 cosmetic surgery Methods 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000015177 dried meat Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000003463 hyperproliferative effect Effects 0.000 description 1
- 230000001969 hypertrophic effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000011505 plaster Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 235000008113 selfheal Nutrition 0.000 description 1
- 238000007390 skin biopsy Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000009056 telangiectasis Diseases 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L83/00—Compositions of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon only; Compositions of derivatives of such polymers
- C08L83/04—Polysiloxanes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2383/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen, or carbon only; Derivatives of such polymers
- C08J2383/04—Polysiloxanes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/02—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
- C08L2205/025—Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2205/00—Polymer mixtures characterised by other features
- C08L2205/03—Polymer mixtures characterised by other features containing three or more polymers in a blend
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a hyaluronic acid silica gel and a preparation method thereof, wherein the hyaluronic acid silica gel is prepared from the following components in parts by mass: 10 to 50 percent of polydimethylsiloxane, 1 to 10 percent of emulsifier, 1 to 20 percent of glycerin, 0.01 to 10 percent of sodium hyaluronate and 28.99 to 69 percent of purified water. The hyaluronic acid silica gel provided by the invention can effectively inhibit excessive proliferation of fibroblasts, and simultaneously provides a wet environment for scars, so that the formation of scars is inhibited. The effect of repairing scars is remarkable. Meanwhile, the hyaluronic acid silica gel provided by the invention is simple in preparation method and low in production cost.
Description
Technical field
The present invention relates to a kind of biomedicine field, relate generally to a kind of hyaluronic acid Silica hydrogel group that cicatrix is had repair
Compound and preparation thereof.
Technical background
Cicatrix is that the reason such as physics, biochemistry causes human body skin soft tissue major injury the most normally not repair,
Turning by fibrous tissue replacement, staying and affecting function affects again the local symptom of outward appearance.Cicatrix growth exceedes certain limit, just
Various complication can occur, the destruction of such as profile and functional activity obstacle etc., bring the huge human body painful and spiritual to patient
Misery, the cicatrix especially burnt, scald, leave over after severe trauma.Several years of scar hyperplasia phase almost allow patient's hardship not
May say.The atrophy phase then makes again patient changed beyond recognition, dysfunction, causes the great body of patient, heart double combination obstacle.Along with
The progress of modern society, likes to be beautiful and not only represents a kind of right, also illustrates that a kind of respect to oneself, and cicatrix is drainer for plastic surgery
One of modal problem in outpatient service, except the impact of outward appearance, in fact has many cicatrixes to have and painful scratches where it itches, aches
Pain and dry and cracked, produces scar contracture if unfortunate, more can affect that extremities joint is movable or the normal function of face, these scars further
The treatment of trace non-positive especially can not.
Existing market is the Silica hydrogel scar plaster or Silica hydrogel made with single polysiloxanes mostly, but this series products is to cicatrix
Repair simply passively locks water, does not have the moisture-keeping functions that actively absorbs water, and cicatrix repairing effect is not ideal enough.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of hyaluronic acid Silica hydrogel that can effectively remove scar.
Second object of the present invention is to provide the preparation method of a kind of preparation technology simple hyaluronic acid Silica hydrogel.
Technical scheme is summarized as follows:
A kind of hyaluronic acid Silica hydrogel, is made up of following component by quality: polydimethylsiloxane 10%-50%, emulsifying agent 1%-10%,
Glycerol 1%-20%, hyaluronate sodium 0.01%-10% and purified water 28.99%-69%.
Described compositions includes following composition by mass percentage: polydimethylsiloxane 30%, emulsifying agent 4%, glycerol 10%,
Hyaluronate sodium 0.3% and purified water 55.7%.
Described hyaluronate sodium is 1:(1～4 by mass ratio): the molecular weight ranges of (1～20) is 1000000～2000000
Macromolecule hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 100000～400000 and molecular weight ranges exist
The micromolecule hyaluronic acid sodium composition of 5000～10000.
Described hyaluronate sodium by the molecular weight ranges that mass ratio is 1:4:10 1400000～1600000 macromolecule transparent
Matter acid sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 200000～300000 and molecular weight ranges 7000～8000 little
Numerator sodium hyaluronate forms.
Described emulsifying agent is octyldimethyl siloxanes ethyoxyl glucoside, PEG-9 polydimethylsiloxanes ethyl poly dimethyl silicon
Oxygen alkane, lauryl KF-6028 and polyglycereol-3 disiloxane polydimethylsiloxane
In one.
The preparation method of a kind of hyaluronic acid Silica hydrogel, comprises the steps:
1) weigh raw material by mass percentage: polydimethylsiloxane 10%-50%, emulsifying agent 1%-10%, glycerol 1%-20%,
Hyaluronate sodium 0.01%-10% and purified water 10%-87.99%；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 80 90 DEG C under conditions of being 10-20 rev/min, keep 10-20 minute at 80 90 DEG C, obtain oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 80-90 DEG C, keep 10-20 minute at 80 90 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 500-800 second under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Described step (1) is: weigh raw material by mass percentage: polydimethylsiloxane 30%, emulsifying agent 4%, glycerol 10%,
Hyaluronate sodium 0.3% and purified water 55.7%.
Described hyaluronate sodium is 1:(1～4 by mass ratio): the molecular weight ranges of (1～20) is 1000000～2000000
Macromolecule hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 100000～400000 and molecular weight ranges exist
The micromolecule hyaluronic acid sodium composition of 5000～10000.
Described hyaluronate sodium by the molecular weight ranges that mass ratio is 1:4:10 1400000～1600000 macromolecule transparent
Matter acid sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 200000～300000 and molecular weight ranges 7000～8000 little
Numerator sodium hyaluronate forms.
Described emulsifying agent is octyldimethyl siloxanes ethyoxyl glucoside, PEG-9 polydimethylsiloxanes ethyl poly dimethyl silicon
Oxygen alkane, lauryl KF-6028 and polyglycereol-3 disiloxane polydimethylsiloxane
In one.
A kind of hyaluronic acid Silica hydrogel of the present invention, it is possible to effectively suppression fibroblast hyper-proliferative, provides moist for cicatrix simultaneously
Environment, thus suppress the formation of cicatrix.The effect repairing cicatrix is notable.Meanwhile, prepared by the hyaluronic acid Silica hydrogel of this present invention
Method is simple, and production cost is relatively low.
Detailed description of the invention
Below by specific embodiment, the present invention is further illustrated.
Embodiment 1
A kind of hyaluronic acid Silica hydrogel, is made up of following component by quality: polydimethylsiloxane 10%, PEG-9 poly dimethyl silicon
Oxygen ethyl polydimethylsiloxane (emulsifying agent) 10%, glycerol 1%, hyaluronate sodium 10% and purified water 69%.
The hyaluronate sodium of the present embodiment by the molecular weight ranges that mass ratio is 1:2:8 1000000～1200000 macromolecule
Hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 300000～400000 and molecular weight ranges are 5000～7000
Micromolecule hyaluronic acid sodium composition.
Embodiment 2
A kind of hyaluronic acid Silica hydrogel, is made up of following component by quality: polydimethylsiloxane 50%, and lauryl PEG-9 gathers
Dimethyl silica ethyl polydimethylsiloxane (emulsifying agent) 1%, glycerol 20%, hyaluronate sodium 0.01% and purified water 28.99%.
The hyaluronate sodium of the present embodiment by the molecular weight ranges that mass ratio is 1:1:1 1800000～2000000 macromolecule
Hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 100000～200000 and molecular weight ranges are 8000～10000
Micromolecule hyaluronic acid sodium composition.
Embodiment 3
A kind of hyaluronic acid Silica hydrogel, is made up of following component by quality: polydimethylsiloxane 30%, octyldimethyl silica
Alkane ethyoxyl glucoside (emulsifying agent) 4%, glycerol 10%, hyaluronate sodium 0.3% and purified water 55.7%.
The hyaluronate sodium of the present embodiment by the molecular weight ranges that mass ratio is 1:4:10 1400000～1600000 macromole
Amount hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 200000～300000 and molecular weight ranges are 7000～8000
Micromolecule hyaluronic acid sodium composition.
Embodiment 4
A kind of hyaluronic acid Silica hydrogel, is made up of following component by quality: polydimethylsiloxane 25%, polyglycereol-3 two silica
Alkane polydimethylsiloxane (emulsifying agent) 7%, glycerol 12%, hyaluronate sodium 4% and purified water 52%.
The hyaluronate sodium of the present embodiment by the molecular weight ranges that mass ratio is 1:4:20 1700000～1800000 macromole
Amount hyaluronate sodium, the Middle molecule hyaluronate sodium of molecular weight ranges 200000～400000 and molecular weight ranges are 6000～8000
Micromolecule hyaluronic acid sodium composition.
Embodiment 5
The preparation method of a kind of hyaluronic acid Silica hydrogel, comprises the steps:
1) raw material is weighed by embodiment 1；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 85 DEG C under conditions of being 20 revs/min, keeps 15 minutes at 85 DEG C, obtains oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 80 DEG C, keep 20 minutes at 80 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 500 seconds under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Embodiment 6
The preparation method of a kind of hyaluronic acid Silica hydrogel, comprises the steps:
1) raw material is weighed by embodiment 2；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 80 DEG C under conditions of being 10 revs/min, keeps 20 minutes at 80 DEG C, obtains oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 90 DEG C, keep 20 minutes at 90 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 800 seconds under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Embodiment 7
The preparation method of a kind of hyaluronic acid Silica hydrogel, comprises the steps:
1) raw material is weighed by embodiment 3；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 90 DEG C under conditions of being 15 revs/min, keeps 10 minutes at 90 DEG C, obtains oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 85 DEG C, keep 10 minutes at 85 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 600 seconds under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Embodiment 8
The preparation method of a kind of hyaluronic acid Silica hydrogel, comprises the steps:
1) raw material is weighed by embodiment 4；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 84 DEG C under conditions of being 17 revs/min, keeps 12 minutes at 84 DEG C, obtains oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 83 DEG C, keep 18 minutes at 83 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 700 seconds under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Embodiment 9
The preparation of matched group and preparation:
Compare 1 group: buy commercially available Silica hydrogel dressing (Shanghai food medicine prison tool (accurate) word 2012 the 2640953rd)
Compare 2 groups: the embodiment 12 in Chinese patent 2011103847533 (denomination of invention scar repair material) makes
Scar repair material, its preparation method is as follows: be under agitation dissolved in 80g water by the chitosan lactate 4g of deacetylation 85%,
Add the polyoxyethylene 20 sorbitan monolaurate 2g of emulsification, wetting agent glycerol 8g, mixing, obtain transparent gel
Shape solution；Under army, kinematic viscosity is that the polydimethylsiloxane 50g of 100000cst joins in described gelatinous solution,
Mix homogeneously, i.e. obtains a kind of scar repair material.
Compare 3 groups: take by quality: 30g polydimethylsiloxane, 4g octyldimethyl siloxanes ethyoxyl glucoside, 10g
Glycerol, 0.3g molecular weight is the hyaluronate sodium of 1400000～1600000, and 55.7g purified water makes according to embodiment 7
Bright matter acid Silica hydrogel.
Compare 4 groups: take by quality: 30g polydimethylsiloxane, 4g octyldimethyl siloxanes ethyoxyl glucoside, 10g
Glycerol, 0.3g molecular weight is the hyaluronate sodium of 200000～300000, and 55.7g purified water makes transparent according to embodiment 7
Matter acid sodium Silica hydrogel.
Compare 5 groups: take by quality: 30g polydimethylsiloxane, 4g octyldimethyl siloxanes ethyoxyl glucoside, 10g
Glycerol, 0.3g molecular weight is the hyaluronate sodium of 6000～8000, and 55.7g purified water makes hyaluronic acid according to embodiment 7
Sodium Silica hydrogel.
Embodiment 10
Wettability test
Moist test direction principle is drawn with reference to " 2015 editions Chinese Pharmacopoeias (the 4th) " guideline 9103 medicine
1, take dry tool plug glass weighing botle (external diameter is 50mm, a height of 15mm) and be placed in suitable 25 DEG C ± 1 the previous day
DEG C thermostatic drier (ammonium chloride or ammonium sulfate saturated solution are placed in bottom) or growth cabinet (design temperature is 25 DEG C ± 1 DEG C,
Relative humidity is 80% ± 2%) in, precise weighing (m1)。
2. the hyaluronate sodium Silica hydrogel that prepared by Example 5-8, compare 1 group, compare 3 groups, compare 4 groups, compare 5 groups,
Trehalose, hyaluronate sodium sodium are appropriate, put in above-mentioned weighing botle and are laid in weighing botle, and test sample thickness is typically about 1mm,
Precise weighing (m2)。
Weighing botle is uncovered 3., and with bottle cap with being placed under the conditions of above-mentioned constant temperature and humidity 24 hours.
4. build weighing botle lid, precise weighing (m3)。
Percentage weight increase=(m3-m2)/(m2-m1) × 100%
5. draw moist feature description and draw defining of moist weightening finish.
Deliquescence: absorb enough water and divide formation liquid.
Great draw moist: draw wet weightening finish not less than 15%.
Have draw moist: draw wet weightening finish less than 15% but not less than 2%.
Slightly draw moist: draw wet weightening finish less than 2% but not less than 0.2%.
Nothing or moist almost without drawing: draw wet weightening finish less than 0.2%.
Table 1, draw moist experimental result
| Percentage weight increase (%) | Draw moist | |
| Embodiment 5 | 12.15% | Have draw moist |
| Embodiment 6 | 13.36% | Have draw moist |
| Embodiment 7 | 15.16% | Great draw moist |
| Embodiment 8 | 13.64% | Have draw moist |
| Compare 1 group | 1.67% | Slightly draw moist |
| Compare 3 groups | 8.19% | Have draw moist |
| Compare 4 groups | 7.61% | Have draw moist |
| Compare 5 groups | 6.38% | Have draw moist |
| Trehalose | 4.29% | Have draw moist |
| Hyaluronate sodium | 5.78% | Have draw moist |
Experiment shows, embodiment 7 is great draw moist, embodiment 5,6,8 groups, compare 3-5 group, trehalose and hyaluronate sodium
Have and draw moist, and compare 1 group and slightly draw moist, it was demonstrated that simple Silica hydrogel patch does not have the moisture-retaining capacity of actively water suction.That is,
The product of the present invention can actively absorb water moisturizing, repairs one moistening environment of offer for cicatrix.And can be found out by experiment
Effective than other of embodiment 7.
Embodiment 11
CK skinanalysis apparatus is used to measure skin moisture-keeping degree.
Volunteer cleans arm, dries, and measures the basic value of now skin, takes test products 0.05g uniform application in 1cm2's
On skin, measure 0h, 1h, 2h, 4h skin moisture-keeping.
Test and be divided into following groups:
Embodiment 5 groups；
Embodiment 6 groups；
Embodiment 7 groups；
Embodiment 8 groups；
Compare 1 group；
Compare 2 groups；
Compare 3 groups；
Compare 4 groups；
Compare 5 groups；
Table 2, moistening effect result of the test
*Compare with compareing 1 group, P ＜ 0.01
As seen from Table 2, embodiment 5,6,7,8 groups is better than compareing 1,2,3,4,5 groups.
Embodiment 12
Inhibitory action to rabbit ear hypertrophic cicatrix.
Choosing 100 cleaning grade New Zealand large ear rabbits, male and female half and half, body weight 2.5-3kg, Changsha sky duty biotechnology is limited
Company.White rabbit adaptability is fed 48 hours.Use Su Mian Xin 0.2ml/kg intramuscular injection anesthesia, rabbit ear facies ventralis skin 75% wine
Essence sterilization, operates under stringent asepsis requirements, excises epidermis, corium and perichondrium, cartilage-preserving with skin biopsy apparatus and scalpel.
Every ear prepares the standard wound surface of 6 diameter 2cm.Postoperative wound surface exposes, and treats its self-heal, the Hypertrophic scar of the rabbit ear after about 23 days
Trace initially forms, and 100 white rabbits is randomly divided into 10 groups, is grouped as follows:
Embodiment 5 groups；
Embodiment 6 groups；
Embodiment 7 groups；
Embodiment 8 groups；
Compare 1 group；
Compare 2 groups；
Compare 3 groups；
Compare 4 groups；
Compare 5 groups；
Blank group.
Within postoperative 23 days, rise, partial smearing test products at hypertrophic cicatrix, every day 2 times, after postoperative 49 days, white rabbit put to death,
Cut hypertrophic cicatrix tissue specimen.
One. cicatrix thickness measurement: the 21st, 28,35,42,49 days after surgery, record the size of hypertrophic cicatrix, color,
Hardness, use slide gauge simultaneously, measure the thickness of cicatrix after being administered.
Table 3, cicatrix thickness measurement (mm)
| Group | 21 days | 28 days | 35 days | 42 days | 49 days |
| Compare 1 group | 1.08 | 1.40 | 1.79* | 1.92* | 2.32* |
| Compare 2 groups | 1.08 | 1.37 | 1.74* | 1.89* | 2.17* |
| Compare 3 groups | 1.06 | 1.38 | 1.70* | 1.87* | 2.11* |
| Compare 4 groups | 1.07 | 1.37 | 1.67* | 1.83* | 2.10* |
| Compare 5 groups | 1.07 | 1.38 | 1.66* | 1.85* | 2.13* |
| Embodiment 5 groups | 1.06 | 1.31 | 1.51*△ | 1.60*△ | 1.89*△ |
| Embodiment 6 groups | 1.07 | 1.32 | 1.50*△ | 1.61*△ | 1.88*△ |
| Embodiment 7 groups | 1.08 | 1.30 | 1.49*△ | 1.59*△ | 1.81*△ |
| Embodiment 8 groups | 1.05 | 1.32 | 1.51*△ | 1.62*△ | 1.87*△ |
| Blank | 1.08 | 1.57 | 2.34 | 2.98 | 3.27 |
* P < 0.01 is compared with blank；△ P < 0.05 with compare compared with in the of 1 group
Start that hypertrophic cicatrix occurs after each group rabbit ear wound surface postoperation recovery, and the lump constantly exceeding surface can be contacted, Hypertrophic
Cicatrix initially forms the time and is about 21 days, it can be seen that embodiment 5,6,7,8 groups with compare 1,2,3,4,5 groups of equal energy
Make rabbit ear hypertrophy thickness slow down (with blank ratio), but embodiment 9,10,11,12 groups becomes apparent from than each matched group effect.
Two. hydroxyproline determination: take postoperative 49 days rabbit ear scar tissue about 30mg and put in 50ml conical flask, add 6M salt
Acid 10ml, is placed at electric drying oven with forced convection 105 DEG C hydrolysis 16 hours, adjusts pH to 6.8-6.9, by hydroxyproline test kit
Be sequentially added into reagent solution, mixing, 60 DEG C of water-baths 15 minutes, after cooling, 3500 revs/min centrifugal 10 minutes, take supernatant and exist
At 550nm, 1cm optical path, survey absorbance.Hydroxyproline content is calculated according to formula in test kit.
Hydroxyproline content in table 4, scar tissue
| Group | Hydroxyproline content |
| Compare 1 group | 5.75±0.31* |
| Compare 2 groups | 5.61±0.29* |
| Compare 3 groups | 5.57±0.30* |
| Compare 4 groups | 5.58±0.30* |
| Compare 5 groups | 5.56±0.31 |
| Embodiment 5 groups | 4.30±0.23*△ |
| Embodiment 6 groups | 4.31±0.24*△ |
| Embodiment 7 groups | 4.28±0.21*△ |
| Embodiment 8 groups | 4.31±0.26*△ |
| Blank | 9.24±0.20 |
* P < 0.01 is compared with blank；△ P < 0.05 with compare compared with in the of 1 group
Hydroxyproline is one of collagen protein characteristic component, and it is excessive with fibroblast that the process of cicatrization is mainly collagen protein
Increment, i.e. hydroxyproline content is the highest, and cicatrix is the most serious.Postoperative 49 days, table 4 showed 5,6,7,8 groups of hydroxyl dried meat of embodiment
Histidine content the most relatively compare 1,2,3,4,5 groups the fewest, there is statistical significance.
Cicatrix is effectively suppressed to generate by a kind of hyaluronic acid Silica hydrogel of the above-mentioned description of test present invention.
Embodiment 13
Clinical trial:
The selection of case: choose 306 example patients, wherein male 128 people, women 178 people altogether, the age be 7-66 year, adult
243 examples, child 63 example；Case is the cicatrix that acne infects, burns, scalds, wound or Post operation wound healing are formed.
Case is randomly divided into:
Embodiment 5 groups；
Embodiment 6 groups；
Embodiment 7 groups；
Embodiment 8 groups；
Compare 1 group；
Compare 2 groups；
Compare 3 groups；
Compare 4 groups；
Compare 5 groups.
Test products is applied on cicatrix for early, middle and late three times every day, massages until fully absorbing on cicatrix with finger；20 days
It it is a course for the treatment of.
Skin lesion color score: crimson or scarlet companion's telangiectasis meter 3 points；Light red, disappear after pressing meter 2 points；It is the reddest,
Some ash dark count 1 point；Normal skin tone meter 0 point.
The height scoring of skin lesion protuberance: height counts 4 points at more than 4mm；4mm >=highly ＞ 2mm counts 3 points；2mm >=highly ＞ 1mm
Count 2 points；Highly≤1mm counts 1 point；Smooth or depression counts 0 point.
Skin lesion hardness is marked: normal meter 0 point, soft (skin is flexible under minimum resistance) counts 1 point；Submissive (
Energy amoebula under pressure) count 2 points；Hard (the most flexible, to be moved into bulk, pressure is had resistance) counts 3 points；Curved
Bent (tissue such as rope form, cicatrix can be shunk back when stretching) meter 4 points；(the permanent cripetura of cicatrix causes maimed and distortion) of contracture
Count 5 points.
Pruritus is marked: acutely or persistence pruritus with scratch meter 3 points；Often have but less violent, meter 2 points can be stood；
Sometimes itch and count 1 point；Without meter 0 point of itching.
Touch a tender spot and mark: have the strongest " hyperpathia ", tenderness meter 3 points when touching；Pain is had when medium hyperpathia, pressing
Feel but meter 2 points can be stood；The inconspicuous meter of pain 1 point when sometimes having pain, pressing；Painless meter 0 point.
By above-mentioned each index scoring addition be cicatrix order of severity overall score, overall score 1-5 is divided into slightly, 6-11 be divided into moderate,
12-18 is divided into severe.
Recruitment evaluation standard: overall score * 100% before curative effect index=(overall score after overall score-treatment before treatment)/treatment.Assessment
By being almost recovered, four standards effective, effective, invalid.
Recovery from illness: therapeutic index >=90%；Effective: therapeutic index 70%-89%；Effective: therapeutic index 30%-69%；Invalid: to treat
Effect index ＜ 30%.Effective percentage by recovery from illness+effective+effectively in terms of.
Table 5, clinical test results
| Group | Case load | Recovery from illness | Effective | Effectively | Invalid | Effective percentage |
| Compare 1 group | 34 | 22 | 2 | 4 | 6 | 82.35% |
| Compare 2 groups | 34 | 22 | 3 | 4 | 5 | 85.29% |
| Compare 3 groups | 34 | 24 | 3 | 3 | 4 | 88.24% |
| Compare 4 groups | 34 | 23 | 2 | 4 | 5 | 85.29% |
| Compare 5 groups | 34 | 23 | 3 | 4 | 4 | 88.24% |
| Embodiment 5 groups | 34 | 25 | 4 | 3 | 2 | 94.12% |
| Embodiment 6 groups | 34 | 25 | 4 | 3 | 2 | 94.12% |
| Embodiment 7 groups | 34 | 26 | 5 | 2 | 1 | 97.06% |
| Embodiment 8 groups | 34 | 25 | 5 | 1 | 3 | 91.18% |
The hyaluronate sodium Silica hydrogel of the present invention is relatively higher than matched group at the effective percentage for the treatment of cicatrix as can be seen from the above table, enters one
Step illustrates that the hyaluronate sodium matched combined of different molecular weight effect in terms for the treatment of cicatrix is better than single a kind of hyaluronic acid
Sodium, and reasonably proportion relation is the most crucial.
Claims (10)
1. a hyaluronic acid Silica hydrogel, is characterized in that being made up of following component by quality: polydimethylsiloxane 10%-50%,
Emulsifying agent 1%-10%, glycerol 1%-20%, hyaluronate sodium 0.01%-10% and purified water 28.99%-69%.
Gel the most according to claim 1, is characterized in that described compositions includes following composition by mass percentage: poly-two
Methylsiloxane 30%, emulsifying agent 4%, glycerol 10%, hyaluronate sodium 0.3% and purified water 55.7%.
Gel the most according to claim 1 and 2, is characterized in that described hyaluronate sodium is 1:(1～4 by mass ratio):
The molecular weight ranges of (1～20) 1000000～2000000 macromolecule hyaluronate sodium, molecular weight ranges 100000～
The Middle molecule hyaluronate sodium of 400000 and molecular weight ranges form at the micromolecule hyaluronic acid sodium of 5000～10000.
Gel the most according to claim 3, is characterized in that described hyaluronate sodium is by the molecular weight model that mass ratio is 1:4:10
Be trapped among 1400000～1600000 macromolecule hyaluronate sodium, molecular weight ranges 200000～300000 Middle molecule transparent
Matter acid sodium and molecular weight ranges form at the micromolecule hyaluronic acid sodium of 7000～8000.
Gel the most according to claim 1 and 2, is characterized in that described emulsifying agent is octyldimethyl siloxanes ethyoxyl Portugal
Polyglycoside, KF-6028, the lauryl PEG-9 poly-diformazan of polydimethylsiloxanes ethyl
One in radical siloxane and polyglycereol-3 disiloxane polydimethylsiloxane.
6. a preparation method for hyaluronic acid Silica hydrogel, is characterized in that comprising the steps:
1) weigh raw material by mass percentage: polydimethylsiloxane 10%-50%, emulsifying agent 1%-10%, glycerol 1%-20%,
Hyaluronate sodium 0.01%-10% and purified water 10%-87.99%；
2) use vacuum homogeneous emulsifying machine, polydimethylsiloxane and emulsifying agent be placed in the oil phase tank of vacuum homogeneous emulsifying machine,
Low whipping speed is heated to 80 90 DEG C under conditions of being 10-20 rev/min, keep 10-20 minute at 80 90 DEG C, obtain oil phase；
3) hyaluronate sodium, purified water and glycerol are put in the aqueous phase tank of described vacuum homogeneous emulsifying machine, under agitation, heating
To 80-90 DEG C, keep 10-20 minute at 80 90 DEG C, obtain aqueous phase；
4) by oil phase and aqueous phase homogenizing 500-800 second under vacuum；
5) it is cooled to room temperature, obtains a kind of hyaluronic acid Silica hydrogel.
Method the most according to claim 6, is characterized in that described step (1) is: weigh raw material by mass percentage: poly-
Dimethyl siloxane 30%, emulsifying agent 4%, glycerol 10%, hyaluronate sodium 0.3% and purified water 55.7%.
8., according to the method described in claim 6 or 7, it is characterized in that described hyaluronate sodium is 1:(1～4 by mass ratio):
The molecular weight ranges of (1～20) 1000000～2000000 macromolecule hyaluronate sodium, molecular weight ranges 100000～
The Middle molecule hyaluronate sodium of 400000 and molecular weight ranges form at the micromolecule hyaluronic acid sodium of 5000～10000.
Method the most according to claim 8, is characterized in that described hyaluronate sodium is by the molecular weight model that mass ratio is 1:4:10
Be trapped among molecular weight ranges 1400000～1600000 macromolecule hyaluronate sodium, molecular weight ranges 200000～300000
Middle molecule hyaluronate sodium and molecular weight ranges 7000～8000 micromolecule hyaluronic acid sodium form.
10., according to the method described in claim 6 or 7, it is characterized in that described emulsifying agent is octyldimethyl siloxanes ethyoxyl
Glucoside, KF-6028, lauryl PEG-9 polydimethylsiloxanes ethyl poly-two
One in methylsiloxane and polyglycereol-3 disiloxane polydimethylsiloxane.
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| CN104382843A (en) * | 2014-10-28 | 2015-03-04 | 天津嘉氏堂科技有限公司 | Skin repair gel |
| CN105012228A (en) * | 2015-08-17 | 2015-11-04 | 郑州和济生物科技股份有限公司 | Hyaluronic acid and silica gel composition for scar prevention and early repair and preparation method of hyaluronic acid and silica gel composition |
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| CN101745329A (en) * | 2008-12-01 | 2010-06-23 | 赢创德固赛(中国)投资有限公司 | Method for preparing stable oil-in-water emulsion with high oil phase content and the emulsion prepared and the application thereof |
| CN102921314A (en) * | 2012-10-25 | 2013-02-13 | 贵阳时代沃顿科技有限公司 | Compound reverse osmosis membrane with interpenetrating network desalting layer and preparation method of membrane |
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| CN105012228A (en) * | 2015-08-17 | 2015-11-04 | 郑州和济生物科技股份有限公司 | Hyaluronic acid and silica gel composition for scar prevention and early repair and preparation method of hyaluronic acid and silica gel composition |
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| CN110664660A (en) * | 2019-10-18 | 2020-01-10 | 瑞希(重庆)生物科技有限公司 | Scar-resistant composition and preparation method thereof |
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