CN105785560A - Dark field microscope used in cell culture online observation - Google Patents
Dark field microscope used in cell culture online observation Download PDFInfo
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- CN105785560A CN105785560A CN201610325563.7A CN201610325563A CN105785560A CN 105785560 A CN105785560 A CN 105785560A CN 201610325563 A CN201610325563 A CN 201610325563A CN 105785560 A CN105785560 A CN 105785560A
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- dark field
- field microscope
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- moveable support
- object lens
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- 238000004113 cell culture Methods 0.000 title abstract 3
- 238000005286 illumination Methods 0.000 claims abstract description 35
- 230000007246 mechanism Effects 0.000 claims description 6
- 238000003491 array Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 abstract 3
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 description 21
- 230000008859 change Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000006854 communication Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009432 framing Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000004531 microgranule Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention provides a dark field microscope used in cell culture online observation. The microscope comprises at least: an illumination component, a movable support, a supporting pedestal, an object lens, a photosensitive component, a pipe diameter unit, an A/D conversion unit, a power supply module, and a control system; the illumination component is arranged at the top of the movable support; the movable support is arranged on the supporting pedestal; the pipe diameter unit is connected to a lower part of the movable support; the object lens is arranged on the pipe diameter unit and under the illumination component; the photosensitive component is arranged inside the pipe diameter unit and is used for collecting light source signals of the object lens; the A/D conversion unit is connected with the photosensitive component; the control system is connected with the A/D conversion unit; The power supply module is used for powering the dark field microscope used in cell culture online observation. By means of the microscope, direct, repeated and real-time observation to observed objects can be realized without sampling or preparing specimen.
Description
Technical field
The present invention relates to a kind of dark field microscope, particularly relate to a kind of dark field microscope cultivating online observation for cell.
Background technology
Dark field microscope is mainly by using special dark field condenser lens to make illuminating ray skew not enter object lens, and only the scattering light of sample enters object lens.Thus obtain bright picture on a dark background, contrary with dark field illumination, the light of illumination directly arrives imaging plane, claims bright field illumination.
Tyndall phenomenon (Tyndallphenomenon) refers to when light beam injects colloidal sol, from the side of light beam it can be seen that a shinny light beam, in the communication process of light, when light is irradiated to particle, as particle diameter is slightly less than lambda1-wavelength, scattering of light, each microgranule is then occurred just to become a luminous point, from the side it can be seen that a light beam.
Dark field microscope make use of above-mentioned principle just.The special optical structure of dark field microscope make illumination incident light can not within incident object lens, but, sample is illuminated and sends reflection light.During microscopy, because not observing directly illuminating ray, the planar field of view vertical with optical axis is black dull, can it can be clearly seen that the bright object image formed by scattering light and reflection light in deeply dark background, and image and background cause great contrast.Sample in visual field, is illuminated by oblique ray, can from the various body structure surface scattering of sample and reflection light, it is seen that the bright profile of many organelles, such as nucleus, mitochondrion, vacuole and some inclusions etc..If the cell divided, its all kinds of spindle fibers and chromosome also can be got a glimpse of.
But first major part dark field microscope all has the problem that at present, generally, user must first extract specimen before observation specimen, it is then placed within microscope slide, it is then placed within object lens could observe, operates very loaded down with trivial details, be unfavorable for the observation at any time of specimen.
Summary of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide a kind of efficient, can realize Real Time Observation cultivate the online dark field microscope observed for cell.
nullFor achieving the above object and other relevant purposes，The present invention provides a kind of and cultivates the online dark field microscope observed for cell，Described microscope at least includes: illumination component、Moveable support、Support base、Object lens、Photo-sensitive cell、Caliber unit、A/D converting unit、Power module and control system，Described illumination component is arranged on the top of Moveable support，Described Moveable support is arranged on support base，Described caliber unit is connected to Moveable support bottom，Described object lens are arranged on caliber unit and corresponding to the lower section of illumination component，Described photo-sensitive cell is arranged on the light signal in caliber unit for gathering on object lens，Described A/D converting unit connects photo-sensitive cell，Described control system connects A/D converting unit，Described power module is cultivate the online dark field microscope observed for cell to power.
Preferably, described illumination component includes the lamp socket of circle, and is arranged on lamp socket to surround the illuminating lamp of several concentric arrays, and the described concentrically ringed center of circle is consistent with the home position of lamp socket.
Preferably, described lamp socket is 10-30 centimetre to the distance of object lens.
Preferably, the light source of described illuminating lamp forms dark field with object lens, meets the needs of dark field illumination.
Preferably, described illuminating lamp is LED.
Preferably, described support base includes motor, slide mechanism and base, described slide mechanism includes slide block and slide rail, preferably, described control system connects and controls motor, described motor connection sliding block, described slide block is slidably connected on base through slide rail and can move horizontally, and described Moveable support is arranged on slide block.
Preferably, described caliber unit is slidably connected on Moveable support, and caliber unit connects Moveable support place and is provided with chute, and described caliber unit can slide up and down along chute.
Preferably, described control system includes:
Moveable support control unit, for controlling the movement of Moveable support；
Illumination component control unit, for controlling the switch of illumination component；
Check unit, for checking the content that under current state, object lens observe；
Storage element, for storing the content observed needing to preserve.
Preferably, described Moveable support has hollow structure, and described Moveable support control unit is placed in hollow structure.
Preferably, described photo-sensitive cell is ccd sensor.
As it has been described above, the present invention's cultivates the online dark field microscope observed for cell, have the advantages that
Adopting this device can realize repeatedly observing continuously of sample, but without repeatedly sampling, when observing sample, only moving Moveable support by control system, to be placed between photo-sensitive cell and object lens by sample more convenient to operate and quick every time；Decrease the destruction to sample when making microscopy sample so that testing result is more objective simultaneously.
Accompanying drawing explanation
Fig. 1 shows the overall structure schematic diagram of dark field microscope in the present embodiment；
Fig. 2 is shown as the structural framing figure of control system；
Fig. 3 is shown as dark field illumination principle schematic.
Wherein, 1, lamp socket, 2, Moveable support；3, object lens, 4, photo-sensitive cell, 5, support base, 6, caliber unit, 7, control system, 501, motor, 502, slide block, 503, base.
Detailed description of the invention
By particular specific embodiment, embodiments of the present invention being described below, those skilled in the art the content disclosed by this specification can understand other advantages and effect of the present invention easily.
Refer to Fig. 1-3.Notice, the structure of this specification institute accompanying drawings depicted, ratio, size etc., all only in order to coordinate the disclosed content of description, understand for those skilled in the art and read, it is not limited to the enforceable qualifications of the present invention, therefore do not have technical essential meaning, the adjustment of the modification of any structure, the change of proportionate relationship or size, under not affecting effect that the present invention can be generated by and the purpose that can reach, all should still drop on disclosed technology contents and obtain in the scope that can contain.Simultaneously, in this specification cited as " on ", D score, "left", "right", " centre " and " one " etc. term, it is merely convenient to understanding of narration, and it is not used to limit the enforceable scope of the present invention, the change of its relativeness or adjustment, changing under technology contents without essence, when being also considered as the enforceable category of the present invention.
The present invention provides a kind of and cultivates the online dark field microscope observed for cell, microscope includes but not limited to: illumination component, Moveable support 2, support base 5, object lens 3, photo-sensitive cell 4, caliber unit 6, A/D converting unit, controls system and power module.Illumination component is arranged on Moveable support 2 top, Moveable support 2 is arranged on support base 5, caliber unit 6 is arranged on Moveable support 2 bottom, object lens 3 are arranged on caliber unit 6 and corresponding to the lower section of illumination component, photo-sensitive cell 4 is arranged in caliber unit 6 for gathering the light signal on object lens 3, A/D converting unit connects photo-sensitive cell 4, control system connects A/D converting unit, and power module is cultivate the online dark field microscope observed for cell to power.
Photo-sensitive cell adopts ccd sensor in the present embodiment, and the picture signal that ccd sensor obtains is passed to control system by A/D converting unit.Control system also include setting in a computer check unit, for checking the content that under current state, object lens observe and storage element, for storing the content observed needing to preserve.
In the present embodiment, illumination component is arranged on the top of Moveable support 2, illumination component includes but not limited to the lamp socket 1 of circle and is arranged on the LED surrounding 5 concentric arrays on the lamp socket 1 of circle, object lens are 25 centimetres to the distance of lamp socket, general could be arranged to 10-30 centimetre, each concentric circular includes ten to 20 LED, provides light source for whole device.On each concentric circular, the lamp on circumference can be opened simultaneously or close.
As it is shown on figure 3, illumination component also includes lens, lens are located at the lower section in LED so that the light that LED sends reflects after lens, meet the needs of dark field illumination with this.The angle of the light that illumination component sends and object lens 3 trunnion axis is less than angle A, and described angle A is the light angle with object lens 3 trunnion axis in the bosom of illumination component.
In the present embodiment, support base 5 and include motor 501, slide mechanism and base 503, slide mechanism includes slide block 502 and slide rail, slide block 502 is connected on base 503 through slide rail, motor 501 connection control system is also located at one end of base 503, slide block 502 connects motor 501, and Moveable support 2 is arranged on slide block 502.Those of ordinary skill in the art knows motor 501 and motor 501 common in the market can be adopted to realize, and motor 501 can moving and then the relative position of whole microscopical Moveable support 2 is changed with movable slider 502.
In the present embodiment, caliber unit 6 is slidably connected on Moveable support 2, caliber unit 6 connects Moveable support 2 place and is provided with chute, and caliber unit 6 can slide up and down along chute, and on Moveable support, the relative both sides of 2 arrange through hole, two through holes are respectively provided with screw, when needs regulate and raise caliber unit 6, it is possible to unclamp screw, mobile caliber unit 6, when being adjusted to appropriate location, tighten screw.Certainly the adjustment that the adjustment of this rising and reduction is simply trickle, maximal regulated scope is 5 centimetres.The device of similar buckle known on other markets can certainly be adopted for playing fixing effect.Certainly, control system 7 can also be utilized as required to control the switch of LED, with the needs of satisfied observation.
Referring to Fig. 2, for further optimizing design scheme, control system 7 includes but not limited to illumination component control unit；Moveable support control unit, for instance user can by input specific numerical value control motor so that control Moveable support 2 displacement；Check unit, the person of being used primarily for check under current state by object lens it is observed that content；Storage element, is mainly used in storage user and thinks the content observed needing to preserve, in order to again checking.
In another embodiment, Moveable support 2 has hollow structure, and illumination component control unit is arranged in hollow structure.
Those skilled in the art all understands, system controlled as described above is about the process that illumination component is controlled, and process and the process of the movement of control support base 5 that the picture signal that CCD obtains converts all can utilize computer of the prior art, integrated circuit modules, PLD, other hardware or existing software module to realize.
Adopt this device when observing sample, particularly in cell bags or cell factory during cultured cells, there is big advantage.When observing sample, utilizing control system 7 to move movable supporting frame 2 makes whole culture vessel be placed between illumination component and object lens 3, when observing end, control system 7 is utilized to move Moveable support 2, make it away from culture vessel, then utilize control system to move the position of Moveable support 2 when needing and again observing.
So, the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
Above-described embodiment is illustrative principles of the invention and effect thereof only, not for the restriction present invention.Above-described embodiment all under the spirit and category of the present invention, can be modified or change by any those skilled in the art.Therefore, art has usually intellectual such as modifying without departing from all equivalences completed under disclosed spirit and technological thought or change, must be contained by the claim of the present invention.
Claims (10)
1. cultivate the online dark field microscope observed for cell for one kind, it is characterized in that, described microscope at least includes: illumination component, Moveable support, support base, object lens, photo-sensitive cell, caliber unit, A/D converting unit, power module and control system, described illumination component is arranged on the top of Moveable support, described Moveable support is arranged on support base, described caliber unit is connected to Moveable support bottom, described object lens are arranged on caliber unit and corresponding to the lower section of illumination component, described photo-sensitive cell is arranged on the light signal in caliber unit for gathering on object lens, described A/D converting unit connects photo-sensitive cell, described control system connects A/D converting unit, described power module is cultivate the online dark field microscope observed for cell to power.
2. the dark field microscope cultivating online observation for cell according to claim 1, it is characterized in that: described illumination component includes the lamp socket of circle, and it being arranged on lamp socket to surround the illuminating lamp of several concentric arrays, the described concentrically ringed center of circle is consistent with the home position of lamp socket.
3. the dark field microscope cultivating online observation for cell according to claim 2, it is characterised in that: described lamp socket is 10-30 centimetre to the distance of object lens.
4. the dark field microscope cultivating online observation for cell according to claim 3, it is characterised in that: the light source of described illuminating lamp forms dark field with object lens, meets the needs of dark field illumination.
5. the dark field microscope cultivating online observation for cell according to claim 3, it is characterised in that: described illuminating lamp is LED.
6. the dark field microscope cultivating online observation for cell according to claim 1, it is characterized in that: described support base includes motor, slide mechanism and base, described slide mechanism includes slide block and slide rail, described control system connects and controls motor, described motor connection sliding block, described slide block is slidably connected on base through slide rail and can move horizontally, and described Moveable support is arranged on slide block.
7. the dark field microscope cultivating online observation for cell according to claim 1, it is characterized in that: described caliber unit is slidably connected on Moveable support, caliber unit connects Moveable support place and is provided with chute, and described caliber unit can slide up and down along chute.
8. the dark field microscope cultivating online observation for cell according to claim 1, it is characterised in that: described control system includes:
Moveable support control unit, for controlling the movement of Moveable support；
Illumination component control unit, for controlling the switch of illumination component；
Check unit, for checking the content that under current state, object lens observe；
Storage element, for storing the content observed needing to preserve.
9. the dark field microscope cultivating online observation for cell according to claim 8, it is characterised in that: described Moveable support has hollow structure, and described Moveable support control unit is placed in hollow structure.
10. the dark field microscope cultivating online observation for cell according to claim 1, it is characterised in that: described photo-sensitive cell is ccd sensor.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610325563.7A CN105785560B (en) | 2016-05-16 | 2016-05-16 | Dark field microscope for cell culture on-line observation |
| PCT/CN2016/110035 WO2017107837A1 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
| US16/064,414 US10934513B2 (en) | 2015-12-23 | 2016-12-15 | Fully automated continuous cell culture system |
| JP2018533724A JP6889723B2 (en) | 2015-12-23 | 2016-12-15 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610325563.7A CN105785560B (en) | 2016-05-16 | 2016-05-16 | Dark field microscope for cell culture on-line observation |
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| Publication Number | Publication Date |
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| CN105785560A true CN105785560A (en) | 2016-07-20 |
| CN105785560B CN105785560B (en) | 2022-11-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610325563.7A Active CN105785560B (en) | 2015-12-23 | 2016-05-16 | Dark field microscope for cell culture on-line observation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
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| CN205750090U (en) * | 2016-05-16 | 2016-11-30 | 上海吉凯基因科技有限公司 | The online dark field microscope observed is cultivated for cell |
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2016
- 2016-05-16 CN CN201610325563.7A patent/CN105785560B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101048492A (en) * | 2004-11-09 | 2007-10-03 | 株式会社日立医药 | Cell cultivating device, image processing device and cell detecting system |
| CN102628983A (en) * | 2011-02-03 | 2012-08-08 | 徕卡显微系统(瑞士)股份公司 | Transillumination device for a microscope |
| CN103197413A (en) * | 2013-04-15 | 2013-07-10 | 厦门大学 | Optics microscopic imaging method capable of generating three-dimensional images |
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| CN204575517U (en) * | 2015-04-29 | 2015-08-19 | 南京英恩特环境技术有限公司 | A kind of micro-imaging dew point hygrometer of dark field illumination |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| US10934513B2 (en) | 2015-12-23 | 2021-03-02 | Shanghai GenBase Biotechnology Co., Ltd. | Fully automated continuous cell culture system |
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Application publication date: 20160720 Assignee: HUADAO (SHANGHAI) BIOPHARMA Co.,Ltd. Assignor: SHANGHAI JI KAI GENE TECHNOLOGY Co.,Ltd. Contract record no.: 2017310000066 Denomination of invention: Dark field microscope used in cell culture online observation License type: Exclusive License Record date: 20171207 |
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