CN105784906A - Establishing method of squalene as identification marker of olive oil and camellia seed oil - Google Patents
Establishing method of squalene as identification marker of olive oil and camellia seed oil Download PDFInfo
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Abstract
The invention discloses an establishing method of squalene as an identification marker of olive oil and camellia seed oil. The method includes the steps of firstly, preparing a squalene standard product into different concentrations, detecting peak areas of different concentrations through a gas chromatograph, and calculating the standard curve and the calculation formula of the relation of the peak areas and the concentrations; secondly, conducting high-temperature saponification on camellia seed oil and olive oil extracted from camellia seeds and olive fruit respectively, or a trace sample of commercial oil, extracting squalene with petroleum ether, and detecting the peak areas of squalene in samples through the gas chromatograph to obtain the squalene contents of various samples; finally, conducting differential statistics and analysis on the squalene contents of all camellia seed oil and olive oil to obtain the squalene contents of camellia seed oil and olive oil. The sample preprocessing is simple, data is visual and reliable, and repeatability is high.
Description
Technical field
The present invention relates to oils and fats detection field in food inspection, especially, the present invention relates to a kind of Squalene content and differentiate gas-chromatography detection method and the discrimination standard of foundation as olive oil and camellia seed oil.
Background technology
Olive oil is that one originates in Mediterranean ancient oil kind, there is minimizing cardiovascular and cerebrovascular disease occur, improve the multiple efficacies such as digestion function, anticancer, anti-aging, blood circulation promoting and nervous system development, its nutritive value gains public acceptance already in the world, it is described as " liquid golden ", " vegetable oil queen ".Camellia seed oil is that the China domestic ancient oil extracted from Theaceae tea oil tree seed is planted, and its chemical property, especially fatty acid composition is closely similar with olive oil.Also having significantly high nutritive value because it has prevention effect such as cardiovascular disease, oxidation and removing free radicals, camellia seed oil is described as " east olive oil ", " long life oil ".
China is the original producton location of oil tea, is camellia seed oil manufacturing country maximum in the world, is deeply welcome by domestic consumer, and because of limits throughput, price is in up-trend in recent years.The olive oil of domestic market is mainly from Europe import.Olive oil is divided into Marc oil two class of force feed and solvent extraction, and generally squeezing olive oil is as edible oil, and price is higher, and Marc oil is generally as iundustrial oil, for cosmetics and cosmetics of everyday use, feedstuff, inexpensive, is even significantly less than domestic camellia seed oil.The camellia seed oil that past Chinese market occurs is usually the Petiolus Trachycarpi wet goods that its price is relatively low.In detecting along with edible oil, gas chromatograph is commonly used, by fatty acid profile and content, camellia seed oil is pretended to be to be easy to differentiate with its conventional edible oil, start to pretend to be camellia seed oil with the import poor quality marc olive oil that fatty acid profile and ratio are similar to camellia seed oil for this illegal businessman in recent years, investigated and prosecuted in Zhejiang Province and reinstated marc olive oil more and pretend to be the important case of camellia seed oil.According to Quzhou daily paper on August 8th, 2012, this quality supervision department of city is once for pretending to be the illegal discreditable behavior of camellia seed oil to carry out special administration with olive fruit residual oil." olea europaea fruit residual oil " was also once pretended to be by the focus efforts on special areas camellia seed oil activity of Huoshan County of Anhui Province camellia seed oil as investigating and prosecuting content.But there is not yet the ready-made detection method that can effectively differentiate that camellia seed oil pretended to be by olive oil so far.
Not about the discrimination method of the olive oil that fatty acid composition is similar in existing Chinese national standard " GB11765-2003 camellia seed oil ", existing disclosed patent application there is the content utilizing physical detection means to differentiate olive oil kind and olive oil quality, as adopted the olive oil fast detection method (application number 200810106530.9) of Raman spectrum characteristic peak signal intensity ratio, but in adulterated oils and fats used by it and the discriminating of not mentioned camellia seed oil and olive oil, and required instrument price is expensive, not easily operating, data analysis is loaded down with trivial details.It is related to the patent that camellia seed oil differentiates, such as a kind of simple and quick method (application number CN201010217382.5) differentiating oil-tea camellia seed oil, describe according to this patent application, utilize in camellia seed oil containing steroid component, the principle that this composition and developer can develop the color is to distinguish other vegetable oil without steroidal, the shortcoming of this method is in that olive oil and other vegetable oil containing steroid component cannot use, and chromogenic reaction is accurate not, it is impossible to accomplish quantitative analysis.Document and some standards relate to camellia seed oil and mixes pseudo-discrimination method, principle used is camellia seed oil fatty acid composition, if Yan Xiaoli, Xu Xin are in " adulterated oil Oleum Camelliae qualitative identification-gas chromatography " (" food engineering " 12 phases in 2011), its shortcoming of method of middle such principle of utilization is in that the olive oil being difficult to similar to camellia seed oil fatty acid composition is distinguished.Zhou Jianping and Guo Hua " research of camellia seed oil method for quantitatively determining " " Chinese oil " the 28th volume the 2nd phase 55-57 page in 2003 have employed acetic anhydride-concentrated sulphuric acid colour developing and sends out and differentiate colored complex method in camellia seed oil, but its principles of chemistry are probably reacted with the tea saponin phase remained, but we are verified finding that this method is bad to the camellia seed oil effect that purity is higher, repeatability is bad.
The mensuration being related in camellia seed oil in " in camellia seed oil the gas chromatography determination of Squalene content " (" assay office " the 30th volume o. 11th 104-106 page in 2011) of Zhong Donglian etc. Squalene content, the mensuration being related in olive oil in " different cultivars and the detection analysis of Squalene in Maturity olive oil | " of Geng Shuxiang etc. (" China's grain and oil journal " the 28th volume the 2nd phase 123-129 page in 2013) Squalene content, but these two sections of papers pertain only to the methodology demonstration of Squalene detection, it does not have oils and fats differentiates related fields research contents.Long Zhenghai, kingly way flat " camellia seed oil and olive oil chemical constitution study " (" China's grain and oil journal " the 23rd volume the 2nd phase 121-123 page in 2008) report in olive oil that Squalene content is apparently higher than camellia seed oil, but its adopted sample is commodity, only has 1 sample size respectively, do not have to adopt the reliable method extracting oil product from former fruit, there is no universality and representative meaning, more do not do Method validation.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide a kind of Squalene and differentiate the method for building up of label as olive oil and camellia seed oil.
It is an object of the invention to be achieved through the following technical solutions.
A kind of Squalene differentiates the method for building up of label as olive oil and camellia seed oil, comprises the following steps:
(1) take Squalene standard substance, be configured to gradient solution with chromatographic grade normal hexane, measured by GC, Criterion curve；
(2) olive fruit is crushed, extract olive oil by physical squeezing method；Camellia seed oil is extracted by chemical-solvent method；
(3) microstandard olive oil or camellia seed oil are taken；The sampling amount of olive oil is 5mg, and camellia seed oil is 100mg；
(4) add potassium hydroxide-ethanol solution and sample is carried out high temperature saponification process；
(5) it is cooled to room temperature, after adding saturated nacl aqueous solution, uses the Squalene in petroleum ether extraction sample；
(6) stratification, adds pure water, is washed till neutrality after separation of supernatant；
(7) stratification, adds a small amount of anhydrous sodium sulfate after separation of supernatant；
(8) centrifugal after concussion, take supernatant and dry up；
(9) film is crossed after adding organic solvent redissolution；Described organic solvent is normal hexane, crosses the organic membrane of 0.45 μm；
(10) after nitrogen dries up, the addition normal hexane laggard GC of redissolution detects；
(11) bring Squalene peak area on gas chromatogram into standard curve and calculate Squalene content；
(12) camellia seed oil and olive oil Squalene content are carried out difference analysis；
(13) utilize GC-MS to Squalene in sample and the appearance time of standard substance Squalene, fragmentation pattern and chemical constitution comparison.
The equation of linear regression that standard curve described in step (11) draws: y=0.3662x-1.0791, R2=0.9999.
In described step (11), Squalene content respectively 0.15 ± 0.07mg/g and 6.72 ± 2.05mg/g, p < 0.01 in olive oil and camellia seed oil in olive oil, camellia seed oil.
In described step (4), potassium hydroxide-ethanol solution concentration is 2mol/L, and addition is 5mL, and saponification temperature is 85 DEG C, and saponification time is 60min.
In described step (8), centrifugal speed is 2000r/min, and centrifugation time is 5min.
Being HP-5MS (30m × 0.25mm × 0.25 μm) by chromatographic column used in described step (11), instrument is Agilent7890A type gas chromatogram instrument.
Described method, further GC conditions is: carrier gas: high pure nitrogen；Injector temperature: 250 DEG C；Detector FID temperature 270 DEG C；Column temperature 100 DEG C, 2min, 15 DEG C/min is warming up to 290 DEG C, 5min；Sample size 1 μ L, split ratio 50:1.
A kind of described Squalene differentiates the application of label as olive oil and camellia seed oil, comprise the following steps: from olive oil to be measured or camellia seed oil, take sample carry out high temperature saponification process, use petroleum ether extraction Squalene, the peak area of Squalene in testing sample is obtained with gas chromatograph detection, peak area is substituted into described standard curve, obtains the Squalene content of testing sample；By the Squalene of testing sample with compared with the Squalene content in olive oil or reference oil Oleum Camelliae, it is judged that olive oil to be measured or the Species origin of camellia seed oil or the true and false.
The invention has the beneficial effects as follows:
(1) present invention is with Squalene for label, and olive oil and camellia seed oil are differentiated, method is easy, and the laboratory generally with gas chromatograph can be carried out；
(2) the method is reproducible, reliable results, and recovery of standard addition 100%-106.3%, relative standard deviation is at 0.13%-0.81%.
(3) the method sampling amount is few, greatly reduces the loss of sample, and detection efficiency is high；
(4) the method can provide very big reference to currently without the unified standard differentiating olive oil and camellia seed oil；
(5) the method directly extracts oils and fats from raw material, greatly avoids the impact of squalene content in the course of processing and the artificial factor added.
Accompanying drawing explanation
Below in conjunction with specific embodiments and the drawings, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention rather than the restriction scope of the invention.
Fig. 1 is Squalene standard curve collection of illustrative plates；
Fig. 2 is camellia seed oil and olive oil Squalene content difference analysis；
Fig. 3 extracts camellia seed oil GC-MS collection of illustrative plates；
Fig. 4 extracts Squalene structural formula in camellia seed oil；
Fig. 5 is fresh squeezing olive oil GC-MS collection of illustrative plates；
Fig. 6 is Squalene structural formula in fresh squeezing olive oil；
Fig. 7 is Squalene standard specimen GC-MS collection of illustrative plates；
Fig. 8 is Squalene guide sample structure formula.
Detailed description of the invention
Instant invention demonstrates multiple from carrying and the content of Squalene in commercially available olive oil and camellia seed oil, it is provided that a kind of Squalene differentiates method for building up and the application of label as olive oil and camellia seed oil.The present invention adopts chemical method, using comparatively conventional gas chromatograph as detecting instrument, by simple pretreatment process, can angle of departure zamene preferably, can directly making result by the comparison of peak area to judge, the Squalene concentration in sample or content then can accurately be calculated by the standard curve set up by external standard method, thus olive oil and camellia seed oil are made quantitative identification accurately, method is reproducible, and data are intuitive and reliable.
1, take Squalene standard substance, be configured to gradient solution, detect with gas chromatogram instrument, obtained standard curve and concentration computing formula by the relation of concentration and peak area.
2, extracting camellia seed oil and olive oil from Semen Camelliae and Fructus Canarii albi fruit, camellia seed oil adopts extract by solvents, and Extraction solvent is normal hexane, and olive oil adopts physical squeezing method to extract.
3, take trace (Oleum Camelliae is 100mg, and olive oil is 5mg) testing sample in 30mL test tube, add the 2mol/LKOH-alcoholic solution of 5mL；It is placed in 85 DEG C of water-baths after saponification 1h and removes cooling；Add the saturated NaCl solution of 5mL, add 4mL petroleum ether；Fully stratification after concussion, takes supernatant in test tube, adds 4mL pure water and is washed to neutrality, takes supernatant liquid in another test tube, and add a small amount of anhydrous sodium sulfate；With the centrifugal 5min of 2000r/min after concussion；Transfer supernatant is in new test tube, and nitrogen is blown to no liquid；Added after 2mL normal hexane (analytical pure) fully dissolves film (the organic filter membrane of ф 13*0.45 μm)；Nitrogen is blown to do again, and 100 μ L normal hexane (chromatographically pure) redissolve laggard row gas chromatographic detection.Detecting step is as follows:
4, gas chromatographic analysis: with Agilent7890A type gas chromatograph for determination fatty acid, gather collection of illustrative plates the spectral data that Treatment Analysis gathers with Agilent chem workstation.Chromatographic peak adopts Squalene standard specimen to identify.Quantitative manner adopts area-method, and quantitative approach is external standard method.
5, spectrogram acquisition process: adopt qualitative with the method that standard substance retention time compares, namely qualitative according to relative retention value；Adopt quantified by external standard method, namely by the peak area Criterion curve of variable concentrations Squalene standard sample, bring testing sample peak area into standard curve and can calculate respective concentration.
6, analytical conditions for gas chromatography: chromatographic condition: HP-5MS (30m × 0.25mm × 0.25 μm) capillary column；Carrier gas: high pure nitrogen；Injector temperature: 250 DEG C；Detector (FID) temperature 270 DEG C；Column temperature 100 DEG C (2min), 15 DEG C/min is warming up to 290 DEG C (5min)；Sample size 1 μ L, split ratio 50:1.
7, utilize GC-MS to Squalene in sample and the appearance time of standard substance Squalene, fragmentation pattern and chemical constitution comparison.GC-MS chromatographic condition is identical with GC, and Mass Spectrometry Conditions is: solvent delay 3.5min；Ion source: EI；Ion source temperature: 230 DEG C；Electron energy 70eV；MSD transmission line temperature: 250 DEG C；Squalene qualitative ion m/z69.1 and 221.0.
Embodiment one: Criterion curve
With normal hexane for solvent, compound concentration is the Squalene standard solution of 10mg/L-700mg/L.With Squalene concentration (mg/L) for abscissa, corresponding peak area is that vertical coordinate does standard curve (Fig. 1), the equation of linear regression of Squalene: y=0.3662x-1.0791 (R2=0.9999).Minimum detectability (LOD): with the 3 of baseline noise times of corresponding concentration for standard, this method is 2.38 μ g/mL.
When detecting concrete sample, Squalene peak area substitution regression equation in sample can be calculated and obtains sample concentration, then concentration is substituted into formula calculated below, can convert and obtain corresponding content (μ g/g):
In formula:
X: Squalene content in raw sample oil, unit is milligram every gram (mg/g)；
Cs: Squalene content in sample introduction solution, is substituted into standard curve by Squalene peak area in sample and calculates gained, and unit is milligrams per liter (mg/L)；
V: sample introduction overall solution volume, unit is microlitre (μ L)；
M: testing sample sampling amount during pre-treatment, unit is gram (g).
Embodiment two: sample collecting and Squalene assay
1, Semen Camelliae and olive fruit collection and oils and fats extract
That detects in the present embodiment has 30 kinds of camellia seed oils and olive oil (table 1).Wherein the Semen Camelliae of numbering 1-21 and the olive fruit of numbering 23-26 are provided by Inst. of Subtropical Forestry, Chinese Academy of Forestry Sciences, and Semen Camelliae derives from Jinhua, Zhejiang and Jiangxi, and olive fruit derives from Qing Chuan, Sichuan.
Table 1 detected sample is detailed
Extracting the core after being pulverized by fresh olive fruit, add a small amount of water, centrifugal after fully pulverizing and stirring, supernatant is fresh squeezing olive oil.Take appropriate camellia seed kernel and be placed in 50 DEG C of baking ovens certain time.It is cooled to room temperature after taking out from baking oven, accurate weighing dry after camellia seed kernel 6g, with shears camellia seed kernel shredded and is placed in glass tubing, add normal hexane by solid-liquid ratio 1:5g/mL, under 50 DEG C of conditions of bath temperature, centrifugal layering after ultrasound on extracting 30min, takes supernatant.Supernatant containing camellia seed oil is revolved steaming post-drying to constant weight, namely obtains camellia seed oil.
2, sample pre-treatments
Take trace (Oleum Camelliae is 100mg, and olive oil is 5mg) testing sample in 30mL test tube, add 2mol/LKOH-ethanol (5.6g potassium hydroxide, the 50mL dehydrated alcohol) solution of 5mL；It is placed in 85 DEG C of water-baths after saponification 1h and removes cooling；Add the saturated NaCl solution of 5mL, add 4mL petroleum ether；Fully stratification after concussion, takes supernatant in test tube, adds 4mL pure water and is washed to neutrality, takes supernatant liquid in another test tube, and add a small amount of anhydrous sodium sulfate；With the centrifugal 5min of 2000r/min after concussion；Transfer supernatant is in new test tube, and nitrogen is blown to no liquid；Added after 2mL normal hexane (analytical pure) fully dissolves film (0.45 μm of organic filter membrane)；Nitrogen is blown to do again, sample introduction after 100 μ L normal hexane (chromatographically pure) redissolution.
3, instrument and condition
INSTRUMENT MODEL: Agilent7890A；Chromatographic condition: HP-5MS (30m × 0.25mm × 0.25 μm) capillary column；Carrier gas: high pure nitrogen；Injector temperature: 250 DEG C；Detector (FID) temperature 270 DEG C；Column temperature 100 DEG C (2min), 15 DEG C/min is warming up to 290 DEG C (5min)；Sample size 1 μ L, split ratio 50:1.
4, testing result
The peak area of the Squalene gone out by gas chromatographic detection after pre-treatment is substituted into standard curve, obtains the Squalene concentration in sample, then substitute into formula calculated below,Can converting and obtain corresponding content (μ g/g): in formula: X: Squalene content in sample oil, unit is milligram every gram (mg/g)；Cs: Squalene concentration in sample introduction solution, is substituted into standard curve by Squalene peak area in sample and calculates gained, and unit is milligrams per liter (mg/L)；V: sample introduction overall solution volume, unit is microlitre (μ L)；M: testing sample sampling amount during pre-treatment, unit is gram (g).
In 24 kinds of camellia seed oils, the testing result of Squalene content is as shown in table 2, and in 6 kinds of olive oil, the testing result of Squalene content is as shown in table 3.
Table 2 various camellia seed oil Squalene assay result
The Squalene assay result of the various olive oil of table 3
5, Squalene content analysis in olive oil and camellia seed oil
Undertaken contrasting (Fig. 2) by Squalene content in 24 kinds of camellia seed oils and 6 kinds of olive oil, Squalene content respectively 6.72 ± 2.05mg/g and the 0.15 ± 0.07mg/g of associative list 2 and the visible olive oil of table 3 and camellia seed oil, the Squalene content of olive oil is 44.8 times of camellia seed oil, having significant difference (p < 0.01), this result shows that Squalene is the label of a kind of effective discriminating olive oil and camellia seed oil.
Embodiment two: Squalene mark-on reclaims
Taking 100mg camellia seed oil, carry out sample pre-treatments by the description in specific implementation method, each sample repeats 5 times.Taking two parts of 100mg camellia seed oil sample, the Squalene standard solution added containing 5 μ g and 10 μ g carries out recovery testu respectively simultaneously.Method precision and the response rate are in Table 4.Result indicates that the method response rate is between 100%-106.3%, and relative standard deviation is between 0.13%-0.81%.
Recovery testu result in table 4 camellia seed oil
Embodiment three: GC-MS is to Squalene in sample and standard substance comparison
1, sample pre-treatments
Take trace (Oleum Camelliae is 100mg, and olive oil is 5mg) testing sample in 30mL test tube, add 2mol/LKOH-ethanol (5.6g potassium hydroxide, the 50mL dehydrated alcohol) solution of 5mL；It is placed in 85 DEG C of water-baths after saponification 1h and removes cooling；Add the saturated NaCl solution of 5mL, add 4mL petroleum ether；Fully stratification after concussion, takes supernatant in test tube, adds 4mL pure water and is washed to neutrality, takes supernatant liquid in another test tube, and add a small amount of anhydrous sodium sulfate；With the centrifugal 5min of 2000r/min after concussion；Transfer supernatant is in new test tube, and nitrogen is blown to no liquid；Added after 2mL normal hexane (analytical pure) fully dissolves film (0.45 μm of organic filter membrane)；Nitrogen is blown to do again, sample introduction after 100 μ L normal hexane (chromatographically pure) redissolution.
2, instrument condition
INSTRUMENT MODEL: Agilent6890B；Chromatographic condition: HP-5MSUI (30m × 0.25mm × 0.25 μm) superelevation inertia capillary column；Carrier gas: high-purity helium；Injector temperature: 250 DEG C；Column temperature 100 DEG C (2min), 15 DEG C/min is warming up to 290 DEG C (5min)；Sample size 1 μ L.Mass Spectrometry Conditions: solvent delay 3.5min；Ion source: EI；Ion source temperature: 230 DEG C；Electron energy 70eV；MSD transmission line temperature: 250 DEG C；Squalene qualitative ion m/z69.1 and 221.0.
3, interpretation of result
Camellia seed oil and olive oil are after pre-treatment, and sample is by GC-MS detection (such as Fig. 3 and Fig. 5), and the material the highest with similarity after Data Comparison in mass spectral database is Squalene.Directly enter GC-MS detection (such as Fig. 7) with Squalene standard specimen, it has been found that retention time fits like a glove, and the mass spectrum of Squalene standard substance is shown in Fig. 8, and in sample, the mass spectrum of Squalene is shown in Fig. 4 and Fig. 6.Squalene and the retention time of standard substance Squalene in sample, chemical structural formula are consistent with fragment ion figure as seen from the above.
Claims (8)
1. a Squalene differentiates the method for building up of label as olive oil and camellia seed oil, it is characterised in that comprise the following steps:
(1) take Squalene standard substance, be configured to gradient solution with chromatographic grade normal hexane, measured by GC, Criterion curve；
(2) olive fruit is crushed, extract olive oil by physical squeezing method；Camellia seed oil is extracted by chemical-solvent method；
(3) microstandard olive oil or camellia seed oil are taken；The sampling amount of olive oil is 5mg, and camellia seed oil is 100mg；
(4) add potassium hydroxide-ethanol solution and sample is carried out high temperature saponification process；
(5) it is cooled to room temperature, after adding saturated nacl aqueous solution, uses the Squalene in petroleum ether extraction sample；
(6) stratification, adds pure water, is washed till neutrality after separation of supernatant；
(7) stratification, adds a small amount of anhydrous sodium sulfate after separation of supernatant；
(8) centrifugal after concussion, take supernatant and dry up；
(9) film is crossed after adding organic solvent redissolution；Described organic solvent is normal hexane, crosses the organic membrane of 0.45 μm；
(10) after nitrogen dries up, the addition normal hexane laggard GC of redissolution detects；
(11) bring Squalene peak area on gas chromatogram into standard curve and calculate Squalene content；
(12) camellia seed oil and olive oil Squalene content are carried out difference analysis；
(13) utilize GC-MS to Squalene in sample and the appearance time of standard substance Squalene, fragmentation pattern and chemical constitution comparison.
2. the method for claim 1, it is characterised in that the equation of linear regression that standard curve described in step (11) draws: y=0.3662x-1.0791, R2=0.9999。
3. the method for claim 1, it is characterized in that, in described step (11), Squalene Content respectively 0.15 ± 0.07mg/g and 6.72 ± 2.05mg/g, p < 0.01 in olive oil and camellia seed oil in olive oil, camellia seed oil.
4. the method for claim 1, it is characterised in that in described step (4), potassium hydroxide-ethanol solution concentration is 2mol/L, addition is 5mL, and saponification temperature is 85 DEG C, and saponification time is 60min.
5. the method for claim 1, it is characterised in that in described step (8), centrifugal speed is 2000r/min, and centrifugation time is 5min.
6. the method for claim 1, it is characterised in that being HP-5MS by chromatographic column used in described step (11), instrument is Agilent7890A type gas chromatogram instrument.
7. method as claimed in claim 6, it is characterised in that GC conditions described further is: carrier gas: high pure nitrogen；Injector temperature: 250 DEG C；Detector FID temperature 270 DEG C；Column temperature 100 DEG C, 2min, 15 DEG C/min is warming up to 290 DEG C, 5min；Sample size 1 μ L, split ratio 50:1.
8. a Squalene as claimed in claim 1 differentiates the application of label as olive oil and camellia seed oil, it is characterized in that, comprise the following steps: from olive oil to be measured or camellia seed oil, take sample carry out high temperature saponification process, use petroleum ether extraction Squalene, the peak area of Squalene in testing sample is obtained with gas chromatograph detection, peak area is substituted into described standard curve, obtains the Squalene content of testing sample；By the Squalene of testing sample with compared with the Squalene content in olive oil or reference oil Oleum Camelliae, it is judged that olive oil to be measured or the Species origin of camellia seed oil or the true and false.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017173638A1 (en) * | 2016-04-07 | 2017-10-12 | 浙江大学 | Method for using squalene as identification marker of olive oil and camellia seed oil |
| CN115010572A (en) * | 2022-07-07 | 2022-09-06 | 广州美林生态科技有限公司 | Method for extracting squalene and vitamin E |
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| WO2017173638A1 (en) * | 2016-04-07 | 2017-10-12 | 浙江大学 | Method for using squalene as identification marker of olive oil and camellia seed oil |
| CN115010572A (en) * | 2022-07-07 | 2022-09-06 | 广州美林生态科技有限公司 | Method for extracting squalene and vitamin E |
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