CN103149303A - Rapid determination method of fatty acid and squalene in plant oil - Google Patents
Rapid determination method of fatty acid and squalene in plant oil Download PDFInfo
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- CN103149303A CN103149303A CN2013100833260A CN201310083326A CN103149303A CN 103149303 A CN103149303 A CN 103149303A CN 2013100833260 A CN2013100833260 A CN 2013100833260A CN 201310083326 A CN201310083326 A CN 201310083326A CN 103149303 A CN103149303 A CN 103149303A
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- 235000014113 dietary fatty acids Nutrition 0.000 title claims abstract description 34
- 239000000194 fatty acid Substances 0.000 title claims abstract description 34
- 229930195729 fatty acid Natural products 0.000 title claims abstract description 34
- 150000004665 fatty acids Chemical class 0.000 title claims abstract description 34
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 title claims abstract description 31
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 title claims abstract description 31
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 229940031439 squalene Drugs 0.000 title claims abstract description 31
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title abstract description 17
- 239000010773 plant oil Substances 0.000 title abstract description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 24
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 24
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 238000004458 analytical method Methods 0.000 claims abstract description 11
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000004817 gas chromatography Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 238000010606 normalization Methods 0.000 claims abstract description 6
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 17
- 239000008158 vegetable oil Substances 0.000 claims description 17
- 239000007789 gas Substances 0.000 claims description 11
- 238000003556 assay Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 5
- 239000012159 carrier gas Substances 0.000 claims description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 5
- 239000001307 helium Substances 0.000 claims description 5
- 229910052734 helium Inorganic materials 0.000 claims description 5
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 5
- 238000003825 pressing Methods 0.000 claims description 5
- 239000010453 quartz Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000001228 spectrum Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 claims description 3
- 230000032050 esterification Effects 0.000 abstract description 7
- 238000005886 esterification reaction Methods 0.000 abstract description 7
- 239000002904 solvent Substances 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000000926 separation method Methods 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 abstract description 2
- 238000001819 mass spectrum Methods 0.000 abstract 2
- 238000005070 sampling Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 235000008390 olive oil Nutrition 0.000 description 25
- 239000004006 olive oil Substances 0.000 description 25
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 6
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 6
- VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 6
- BITHHVVYSMSWAG-KTKRTIGZSA-N (11Z)-icos-11-enoic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCC(O)=O BITHHVVYSMSWAG-KTKRTIGZSA-N 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 3
- ZVRMGCSSSYZGSM-CCEZHUSRSA-N (E)-hexadec-2-enoic acid Chemical compound CCCCCCCCCCCCC\C=C\C(O)=O ZVRMGCSSSYZGSM-CCEZHUSRSA-N 0.000 description 3
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 3
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 3
- 239000005642 Oleic acid Substances 0.000 description 3
- 235000021314 Palmitic acid Nutrition 0.000 description 3
- 235000021355 Stearic acid Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229940108623 eicosenoic acid Drugs 0.000 description 3
- BITHHVVYSMSWAG-UHFFFAOYSA-N eicosenoic acid Natural products CCCCCCCCC=CCCCCCCCCCC(O)=O BITHHVVYSMSWAG-UHFFFAOYSA-N 0.000 description 3
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 3
- 235000020778 linoleic acid Nutrition 0.000 description 3
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 3
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 3
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 3
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 3
- 239000008117 stearic acid Substances 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 230000005477 standard model Effects 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
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Abstract
The invention provides a rapid determination method of fatty acid and squalene in plant oil. The method comprises the following steps of: adding n-hexane in the plant oil according to 35-50mL/g of plant oil to fully dissolve the plant oil; adding a mixed solution of potassium hydroxide and methanol according to 5.5-7.5mL/g of plant oil, oscillating for 3-5 minutes, and carrying out centrifugal separation for 5-8 minutes to obtain an upper sampling solution, wherein the concentration of the mixed solution is 2mol/L; and carrying out GC (gas chromatography) and MS (mass spectrum) analysis in a gas supply phase chromatographic instrument, and calculating according to a conventional area normalization method to obtain the components of the fatty acid and the percentage composition of the functional ingredient squalene. The extraction of sample fatty acid and the methyl esterification of the fatty acid are accomplished synchronously, and at the same time, squalene is extracted in a solvent well, so that the method is rapid, simple and less in solvent amount, and has no harm to human health, therefore, the lower detection limit is realized. The method is applicable to the detection of fatty acid and squalene contents in any plant oil.
Description
Technical field
The present invention relates to a kind of plant component assay method, in especially a kind of olive oil, the rapid assay methods of fatty acid and squalene, belong to technical field of analytical chemistry.
Technical background
In plant extract, plant component are analyzed, usually relate to the mensuration of main fatty acid and functional components in vegetable oil.existing technical standard is all with loaded down with trivial details, complicated operation, and need multiple toxic reagent (as the concentrated sulphuric acid, sherwood oil, benzene, ether etc.) the esterification method that coordinates, carry out in earlier stage of olive oil processing, because treatment effect is undesirable, when the olive oil that will process is sent into when analyzing in gas chromatograph, can cause chromatographic peak not occur, or the separating effect of fatty acid and squalene two principal ingredients is undesirable, and when the main fatty acid composition in the separation olive oil and functional components, also need two different capillary posts of polarity, be that fatty acid analysis will use polar column, the analysis of functional components squalene will be used non-polar column, and in the separation olive oil, fatty acid is also different from the earlier stage processing method of squalene, namely separate two kinds of compositions and need two kinds of esterification methods.Thereby be to cause the main cause that the plant extract analysis cost is high, precision of analysis is not enough all the time.Therefore, be necessary fully prior art is improved.
Summary of the invention
For overcoming in prior art, loaded down with trivial details, complex operations that the Plant Oil Fatty Acid Methyl Ester method exists, time-consuming, effort, and use harmful, toxic reagent, cost is high, the deficiencies such as serious waste of resources, the invention provides a kind of convenient, fast, cost is low, and sample only needs once process early stage, can realize the method for separating and assaying of main fatty acid and functional components squalene in vegetable oil with same root chromatogram column.
The present invention realizes by following technical proposal: the rapid assay methods of fatty acid and squalene in a vegetable oil is characterized in that through the following step:
A, the amount of pressing 35 ~ 50mL/g vegetable oil add normal hexane in vegetable oil, vegetable oil is dissolved fully, by the amount of 5.5 ~ 7.5mL/g vegetable oil, adding concentration is the potassium hydroxide of 2mol/L and the mixed solution of methyl alcohol again, vibration 3～5min, centrifuging 5～8min gets upper sample liquid;
B, steps A gained test liquid is supplied gas carry out GC, MS in chromatography and analyze, and calculate routinely the percentage composition of each fatty acid composition and functional components squalene with area normalization method, wherein:
GC analysis condition: a HP-5MS quartz capillary column; Temperature programme: the heating rate by 3 ℃/min is warming up to 300 ℃; 290 ℃ of injector temperatures; Post flow 1.0mL/min; Press 100kPa before post; Sample size 0.5～1.0 μ L; Split ratio 10: 1; Carrier gas is high-purity helium;
MS analysis condition: ionization mode EI; Electron energy 70; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; 150 ℃ of quadrupole rod temperature; Mass range 35 ~ 500; Employing wiley7n.1 standard spectrum storehouse is retrieved qualitative.
Described normal hexane, potassium hydroxide and methyl alcohol are commercial chromatographic grade product.
The gas chromatograph model of described step B is HP6890GC/5973MS gas chromatography-GC-MS.
the present invention has following advantages and effect: when adopting such scheme to measure in vegetable oil main fatty acid and squalene, its esterification method is quick, simple and direct, and the quantity of solvent of using is little, to health without any harm, be easy to recycle, can greatly reduce solvent cost during batch production, the shortening time, reduce unnecessary waste, economize on resources, when using the method simultaneously, the extraction of sample fatty acid and the esterification of fatty acid are synchronously completed, squalene is extracted well in solvent simultaneously, selected nonpolar capillary column is good with separating of each fatty acid methyl ester to squalene in same heating schedule, quantitatively accurately, and realized lower detection limit.Use the content of squalene in GC-MS and squalene standard model method mensuration olive oil, the preci-sion and accuracy of its method is good, can reach needed testing goal, and this method also is applicable to the detection of fatty acid and squalene content in other vegetable oil.
Description of drawings
Fig. 1 is olive oil gas chromatogram of the present invention.
In Fig. 1 as can be known, the inventive method only needs 35min just can complete simultaneously separating of main fatty acid composition and functional components squalene in vegetable oil, good separation, conventional method needs time (three times of times of 35min) more than 3 times could realize separating of main fatty acid and functional components in vegetable oil, does not wherein also comprise changing pillar and equilibration time also has time for sample pretreatment; The esterification time only needs 15min, and the methyl esterification of fatty acid time of conventional method approximately needs 60 ~ 90min; The analysis of a plant oil sample only needs 50 ~ 60min, and conventional method needs 100 ~ 130min, and two kinds of compositions need 200 ~ 260min.
Embodiment
The present invention will be further described below in conjunction with embodiment.
Embodiment 1
The rapid assay methods of fatty acid and squalene in a kind of olive oil is characterized in that through the following step:
A, the amount of pressing 35mL/g olive oil add normal hexane 2ml in the 40mg olive oil, olive oil is dissolved fully, press the amount of 5.5mL/g olive oil, adding concentration is the potassium hydroxide of 2mol/L and the mixed solution 0.3mL of methyl alcohol again, vibration 3min, centrifuging 5min gets upper sample liquid;
B, steps A gained test liquid is supplied gas carry out GC, MS in chromatography and analyze, and calculate routinely the percentage composition of each fatty acid composition and functional components squalene in olive oil with area normalization method, be respectively: total fatty acid content 94.10% (is respectively: gaidic acid 1.05%, palmitic acid 14.14%, linoleic acid 16.01%, oleic acid 60.44%, stearic acid 1.50%, eicosenoic acid 0.29%, arachidic acid 0.30%, the unknown 0.20%, mountain Yu's acid 0.17%); Squalene content 5.90%.
Wherein:
GC analysis condition: a HP-5MS quartz capillary column (30m * 0.32mm * 0.25 μ m); Temperature programme: the heating rate by 3 ℃/min is warming up to 300 ℃; 290 ℃ of injector temperatures; Post flow 1.0mL/min; Press 100kPa before post; Sample size 0.5～1.0 μ L; Split ratio 10: 1; Carrier gas is high-purity helium;
MS analysis condition: ionization mode EI; Electron energy 70; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; 150 ℃ of quadrupole rod temperature; Mass range 35 ~ 500; Employing wiley7n.1 standard spectrum storehouse is retrieved qualitative.
Embodiment 2
The rapid assay methods of fatty acid and squalene in a kind of olive oil is characterized in that through the following step:
A, the amount of pressing 40mL/g olive oil add normal hexane 2mL in the 50mg olive oil, olive oil is dissolved fully, press the amount of 6mL/g olive oil, adding concentration is the potassium hydroxide of 2mol/L and the mixed solution 0.3mL of methyl alcohol again, vibration 4min, centrifuging 6min gets upper sample liquid;
B, steps A gained test liquid is supplied gas carry out GC, MS in chromatography and analyze, and calculate routinely the percentage composition of each fatty acid composition and functional components squalene in olive oil with area normalization method, be respectively: content of fatty acid 91.70% (is respectively: gaidic acid 1.36%, palmitic acid 15.18%, linoleic acid 10.14%, oleic acid 62.33%, stearic acid 1.58%, eicosenoic acid 0.31%, arachidic acid 0.33%, the unknown 0.17%, mountain Yu's acid 0.30%); Squalene content 8.30%.
Wherein:
GC analysis condition: a HP-5MS quartz capillary column (30m * 0.32mm * 0.25 μ m); Temperature programme: the heating rate by 3 ℃/min is warming up to 300 ℃; 290 ℃ of injector temperatures; Post flow 1.0mL/min; Press 100kPa before post; Sample size 0.5～1.0 μ L; Split ratio 10: 1; Carrier gas is high-purity helium;
MS analysis condition: ionization mode EI; Electron energy 70; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; 150 ℃ of quadrupole rod temperature; Mass range 35 ~ 500; Employing wiley7n.1 standard spectrum storehouse is retrieved qualitative.
Embodiment 3
The rapid assay methods of fatty acid and squalene in a kind of olive oil is characterized in that through the following step:
A, the amount of pressing 50mL/g olive oil add normal hexane 2mL in the 60mg olive oil, olive oil is dissolved fully, press the amount of 7.5mL/g olive oil, adding concentration is the potassium hydroxide of 2mol/L and the mixed solution 0.3mL of methyl alcohol again, vibration 5min, centrifuging 8min gets upper sample liquid;
B, steps A gained test liquid is supplied gas carry out GC, MS in chromatography and analyze, and calculate routinely the percentage composition of each fatty acid composition and functional components squalene in olive oil with area normalization method, be respectively: content of fatty acid 95.97% (is respectively: gaidic acid 1.07%, palmitic acid 14.10%, linoleic acid 8.01%, oleic acid 69.99%, stearic acid 1.60%, eicosenoic acid 0.24%, arachidic acid 0.32%, the unknown 0.24%, mountain Yu's acid 0.40%); Squalene content 4.03%.
Wherein:
GC analysis condition: a HP-5MS quartz capillary column (30m * 0.32mm * 0.25 μ m); Temperature programme: the heating rate by 3 ℃/min is warming up to 300 ℃; 290 ℃ of injector temperatures; Post flow 1.0mL/min; Press 100kPa before post; Sample size 0.5～1.0 μ L; Split ratio 10: 1; Carrier gas is high-purity helium;
MS analysis condition: ionization mode EI; Electron energy 70; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; 150 ℃ of quadrupole rod temperature; Mass range 35 ~ 500; Employing wiley7n.1 standard spectrum storehouse is retrieved qualitative.
Claims (3)
1. the rapid assay methods of fatty acid and squalene in a vegetable oil is characterized in that through the following step:
A, the amount of pressing 35 ~ 50mL/g vegetable oil add normal hexane in vegetable oil, vegetable oil is dissolved fully, by the amount of 5.5 ~ 7.5mL/g vegetable oil, adding concentration is the potassium hydroxide of 2mol/L and the mixed solution of methyl alcohol again, vibration 3～5min, centrifuging 5～8min gets upper sample liquid;
B, steps A gained test liquid is supplied gas carry out GC, MS in chromatography and analyze, and calculate routinely the percentage composition of each fatty acid composition and functional components squalene with area normalization method, wherein:
GC analysis condition: a HP-5MS quartz capillary column; Temperature programme: the heating rate by 3 ℃/min is warming up to 300 ℃; 290 ℃ of injector temperatures; Post flow 1.0mL/min; Press 100kPa before post; Sample size 0.5～1.0 μ L; Split ratio 10: 1; Carrier gas is high-purity helium;
MS analysis condition: ionization mode EI; Electron energy 70; 250 ℃ of transmission line temperature; 230 ℃ of ion source temperatures; 150 ℃ of quadrupole rod temperature; Mass range 35 ~ 500; Employing wiley7n.1 standard spectrum storehouse is retrieved qualitative.
2. rapid assay methods as claimed in claim 1, is characterized in that described normal hexane, potassium hydroxide and methyl alcohol are commercial chromatographic grade product.
3. rapid assay methods as claimed in claim 1, is characterized in that the gas chromatograph model of described step B is: HP6890GC/5973MS gas chromatography-GC-MS.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784906A (en) * | 2016-04-07 | 2016-07-20 | 浙江大学 | Establishing method of squalene as identification marker of olive oil and camellia seed oil |
| CN106872607A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of method that high-resolution determines trans-fatty acid in ganoderma lucidum spore oil |
| WO2017173638A1 (en) * | 2016-04-07 | 2017-10-12 | 浙江大学 | Method for using squalene as identification marker of olive oil and camellia seed oil |
| CN107389847A (en) * | 2017-06-05 | 2017-11-24 | 中国农业科学院蜜蜂研究所 | A kind of method of lipid component in quick analysis Bee Pollen |
| CN108663453A (en) * | 2018-05-28 | 2018-10-16 | 贵州茅台酒股份有限公司 | The assay method of squalene content in a kind of sorghum |
| CN109001306A (en) * | 2018-06-01 | 2018-12-14 | 南昌大学 | The prediction technique of squalene and sterol index in a kind of tea oil |
| CN110031558A (en) * | 2019-04-11 | 2019-07-19 | 山东省食品药品检验研究院 | The rapid detection method of Fatty Acids from Vegetable Oil and squalene |
| CN113588854A (en) * | 2020-12-21 | 2021-11-02 | 四川国为制药有限公司 | Method for detecting fatty acid composition |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105784906A (en) * | 2016-04-07 | 2016-07-20 | 浙江大学 | Establishing method of squalene as identification marker of olive oil and camellia seed oil |
| WO2017173638A1 (en) * | 2016-04-07 | 2017-10-12 | 浙江大学 | Method for using squalene as identification marker of olive oil and camellia seed oil |
| CN106872607A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of method that high-resolution determines trans-fatty acid in ganoderma lucidum spore oil |
| CN107389847A (en) * | 2017-06-05 | 2017-11-24 | 中国农业科学院蜜蜂研究所 | A kind of method of lipid component in quick analysis Bee Pollen |
| CN107389847B (en) * | 2017-06-05 | 2020-01-07 | 中国农业科学院蜜蜂研究所 | Method for rapidly analyzing lipid components in bee pollen |
| CN108663453A (en) * | 2018-05-28 | 2018-10-16 | 贵州茅台酒股份有限公司 | The assay method of squalene content in a kind of sorghum |
| CN108663453B (en) * | 2018-05-28 | 2021-02-23 | 贵州茅台酒股份有限公司 | Method for determining content of squalene in sorghum |
| CN109001306A (en) * | 2018-06-01 | 2018-12-14 | 南昌大学 | The prediction technique of squalene and sterol index in a kind of tea oil |
| CN110031558A (en) * | 2019-04-11 | 2019-07-19 | 山东省食品药品检验研究院 | The rapid detection method of Fatty Acids from Vegetable Oil and squalene |
| CN113588854A (en) * | 2020-12-21 | 2021-11-02 | 四川国为制药有限公司 | Method for detecting fatty acid composition |
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