CN105779531B - A kind of method of improved high temperature liquid water preprocessing lignocellulose biolobic material - Google Patents
A kind of method of improved high temperature liquid water preprocessing lignocellulose biolobic material Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000007781 pre-processing Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 title claims abstract description 11
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- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229920005610 lignin Polymers 0.000 claims abstract description 21
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 claims description 6
- 239000007791 liquid phase Substances 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- 239000012074 organic phase Substances 0.000 claims description 4
- 241000609240 Ambelania acida Species 0.000 claims description 3
- 241001520808 Panicum virgatum Species 0.000 claims description 3
- 241000219000 Populus Species 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 239000008346 aqueous phase Substances 0.000 claims description 3
- 239000010905 bagasse Substances 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 244000138286 Sorghum saccharatum Species 0.000 claims description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 239000003570 air Substances 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 238000005191 phase separation Methods 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims description 2
- 239000010907 stover Substances 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 11
- 102000004190 Enzymes Human genes 0.000 abstract description 11
- 229940088598 enzyme Drugs 0.000 abstract description 11
- 230000003301 hydrolyzing effect Effects 0.000 abstract description 9
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- 239000000413 hydrolysate Substances 0.000 abstract description 2
- 238000006467 substitution reaction Methods 0.000 abstract 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 12
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 230000001461 cytolytic effect Effects 0.000 description 3
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 229920001221 xylan Polymers 0.000 description 3
- 150000004823 xylans Chemical class 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 229920002522 Wood fibre Polymers 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000002025 wood fiber Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005815 base catalysis Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000025938 carbohydrate utilization Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 230000002478 diastatic effect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
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- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a kind of methods of improved high temperature liquid water preprocessing lignocellulose biolobic material, this method in hydrolytic process by adding biology base polar non-solute, part water is substituted with biology base polar non-solute, not only increase system Mass and heat transfer effect, lignin from biomass and surface class lignin are also dissolved simultaneously, to realize the effectively hydrolyzing of hemicellulose, the efficient removal of lignin, and reduce water consumption, solvent can recycle；The inherently biomass hydrolysate such as biology base polar non-solute such as tetrahydrofuran, furfural, substitution part water is conducive to raising system mass transfer characteristic and lignin dissolution, solving hemicellulose in the method for high temperature liquid water preprocessing lignocellulose biolobic material can not further hydrolyze, the removal efficiency of lignin is low, the problem of releasing of subsequent fiber element enzyme and cellulose hydrolyzes is hindered, also solves the problems, such as that highly water intensive, cellulase dosage is high.
Description
Technical field:
The present invention relates to the technical fields that biomass is converted to new energy, and in particular to a kind of improved high temperature liquid water is pre-
The method for handling lignocellulose-like biomass.
Background technique:
The biorefinery production energy and high valuable chemicals are carried out using lignocellulose-like biomass, for ensureing state
Family's energy and economic security have positive effect, and have for slowing down the environmental pollution got worse also highly important existing
Sincere justice.By in lignocellulose-like biomass glycosyl high molecular polymer-hemicellulose and cellulose be hydrolyzed to and can ferment
Property sugar, be production cellulosic ethanol, butanol, the derived energy chemical products such as biogasoline, and then realize the indispensability basis of biorefinery.
But the close space three-dimensional structure of cellulose-hemicellulose-lignin constitutes the natural anti-rotation screen of wood fiber biomass
Barrier removes lignin/hemicellulose steric hindrance by preconditioning technique, increases surface of material aperture and surface area, reduction cellulose
Crystallinity and the degree of polymerization, and then broken biological matter converts barrier, for improving the more of cellulase hydrolysis rate and subsequent microorganism
Sugar utilization is most important.
High-temperature liquid state water pretreatment (Liquid Hot Water) refers to using temperature between 150 DEG C -350 DEG C, pressure
Compressed liquid water higher than its saturated vapor pressure is the method for reaction medium.Because itself H+ and OH- concentration is higher by about than normal water
101.5 times and itself has the function of acid-base catalysis.Compared with the chemical pretreatments such as traditional soda acid, ammonia fiber explosion, this method without
Chemical reagent addition, and catabolite is few, thus have become the research hotspot of preprocessing technical field.However document [Bioresource
Technology144 (2013) 210-215] in the shortcomings that mentioning high-temperature liquid state water pretreatment there are highly water intensive, highly energy-consumings, it is such as sweet
Bagasse handles 20min under 160 DEG C, 5% concentration, and residue cellulase hydrolysis rate is 76.3%, when further decreasing water consumption,
Residue enzymatic hydrolyzation is only 22.1% under 20% concentration；The reduction of water reduces the Mass and heat transfer efficiency of reaction system, in biology
Matter and high temperature liquid water contact interface, hemicellulose is hydrolyzed to xylan, but xylan can not be diffused into water phase further
Hydrolysis, be trapped in biological surface xylan can further polymerization reaction generate class lignin product, on the one hand inhibit partly
The hydrolysis of cellulose, the releasing on the other hand hindering subsequent fiber element enzyme and cellulose hydrolyze.In addition, high concentration of substrate is located in advance
Hemicellulose conversion coefficient is lower during reason, is respectively 74.2% and 28.1% under 5% and 20% concentration.If in preprocessing process
Most of hemicellulose cannot be removed, subsequent residue enzymatic hydrolysis certainly will be will lead to, pentose/hexose common fermentation difficulty increases.
At the same time, low delignification rate makes enzyme dosage during residue cellulase hydrolysis high, these limit this
Popularization and application of the green preconditioning technique in lignocellulose-like biomass diastatic fermentation field.
Summary of the invention:
The object of the present invention is to provide a kind of method of improved high temperature liquid water preprocessing lignocellulose biolobic material,
By adding biology base polar non-solute in hydrolytic process, the mass transfer characteristic of system is not only improved, also while dissolving life
Lignin and surface class lignin, to realize the efficient removal of the effectively hydrolyzing of hemicellulose, lignin, and drop in substance
Low water usage amount.The inherently biomass hydrolysate such as biology base polar non-solute such as tetrahydrofuran, furfural substitutes part
Water is conducive to raising system mass transfer characteristic and lignin dissolution, solves high temperature liquid water preprocessing lignocellulose class biology
Hemicellulose can not be hydrolyzed further in the method for matter, and the removal efficiency of lignin is low, hinder subsequent fiber element enzyme and cellulose
Releasing hydrolysis the problem of, also solve the problems, such as that highly water intensive, cellulase dosage is high.
The present invention is achieved by the following technical programs:
A kind of method of improved high temperature liquid water preprocessing lignocellulose biolobic material, this method includes following step
It is rapid:
It is that 100-200 mesh lignocellulose-like biomass is added in hydrolysis reactor by granularity, volumetric concentration, which is added, is
Biology base polar non-solute/water mixed liquid of 1%-70%, substrate mass concentration are 10%~20%, are stirred evenly,
5-60min is hydrolyzed at 160-200 DEG C, product is separated by solid-liquid separation after reaction, and most of cellulose and part wood are retained in solid phase
Quality, obtained liquid phase carry out organic phase/aqueous phase separation again, and hemicellulose derived carbohydrate rich in, has in obtained water phase
Machine mutually recycles to obtain biology base polar non-solute；Solid phase residue pH4.8 carries out cellulose hydrolyzation 48h, and every gram of substrate adds
Add 5~15FPU of cellulase, obtains the liquid phase rich in glucose and the solid residue rich in lignin.
The biology base polar non-solute includes tetrahydrofuran, furfural etc..
Preferably, according to the property of different lignocellulose-like biomass, be passed through in the reactor ammonia, carbon dioxide,
One of sulfur dioxide, oxygen, air, nitrogen pressurize, so that reaction environment is alkalinity or acidity or oxidation or inertia
Environment.
Preferably, the biology base polar non-solute of recycling is recycled as the reaction solution of next round hydrolysis.
The lignocellulose-like biomass is selected from sweet sorghum slag, bagasse, poplar bits, corncob, corn stover, wheat straw
Stalk, straw, switchgrass, energy grass etc..
The features of the present invention and it is a technical advantage that:
(1) it by adding biology base polar non-solute in hydrolytic process, is replaced with biology base polar non-solute
For part water, system Mass and heat transfer effect is not only increased, also while dissolving lignin from biomass and surface class lignin, from
And realize the efficient removal of the effectively hydrolyzing of hemicellulose, lignin, and reduce water consumption, solvent, which can recycle circulation, to be made
With；
(2) this method combines the dissolution characteristics of high temperature liquid water self-catalysis and polar non-solute, realizes half fiber
Dimension element is molten altogether with lignin, reduces the dosage of subsequent fiber element enzyme；
(3) compared with conventional high-temperature liquid water law, not only delignification rate increases, but also obtained hemicellulose is derivative
Sugared concentration is high, is conducive to subsequent transformation and utilizes；
(4) compared with conventional organic solvents method, during hemicellulose derived carbohydrate is soluble in the aqueous phase, lignin is dissolved in organic phase,
Product separation process is simpler, and the inherently biomass by hydrolyzation such as biology base polar non-solute such as tetrahydrofuran, furfural
Product, process are more green.
Specific embodiment:
It is to further explanation of the invention, rather than limiting the invention below.
Embodiment 1: high temperature liquid water/Tetrahydrofuran System
Using switchgrass as raw material, it is crushed to 100-200 mesh, is launched in intermittent stirring reaction under high pressure narrow-necked earthen jar, is pressed
It is tetrahydrofuran/water mixed liquid that 10% addition volumetric concentration is 1% according to substrate mass concentration, closed reaction system is heated to
165~175 DEG C of speeds of agitator are 500rpm, and being passed through carbon dioxide and keeping reaction pressure is that 3Mpa hydrolyzes 40min, and reaction terminates
Afterwards, collect reactant be separated by solid-liquid separation, solid-phase component analysis it is found that cellulose retention rate be 90.1%, delignification rate
It is 74.5%, liquid phase is directly distilled to recover tetrahydrofuran and is recycled as next reaction solution, and remaining water phase is through high performance liquid chromatography
Analysis is about not added with 3 times of tetrahydrofuran situation it is found that total xylose (including xylose and xylo-oligosaccharide) rate of recovery is 81.8%
(high temperature liquid water treatment conditions are identical).Solid phase residue is added cellulolytic enzyme and is hydrolyzed, and substrate mass concentration is 10%,
After hydrolyzing 48h under the conditions of enzyme concentration 5FPU/g, pH4.8,50 DEG C, 150rpm, cellulase hydrolysis rate is 82.5%, is about not added with
Tetrahydrofuran handles 4 times of residue enzymatic hydrolyzation.
Embodiment 2: high temperature liquid water/Tetrahydrofuran System
Using straw as raw material, it is crushed to 100-200 mesh, is launched in intermittent stirring reaction under high pressure narrow-necked earthen jar, according to
Substrate mass concentration is tetrahydrofuran/water mixed liquid that 15% addition volumetric concentration is 70%, and closed reaction system is heated to
175~185 DEG C of speeds of agitator are 500rpm, and being passed through nitrogen and keeping reaction pressure is that 4Mpa hydrolyzes 30min, after reaction, are received
Collection reactant is separated by solid-liquid separation, it is found that cellulose retention rate is 94.4%, delignification rate is for solid-phase component analysis
82.3%, liquid phase is directly distilled to recover tetrahydrofuran and is recycled as next reaction solution, and remaining water phase is through high performance liquid chromatography point
Analysis is about not added with 4 times of tetrahydrofuran situation it is found that total xylose (including xylose and xylo-oligosaccharide) rate of recovery is 89.2%
(high temperature liquid water treatment conditions are identical).Solid phase residue is added cellulolytic enzyme and is hydrolyzed, and substrate mass concentration is 10%,
After hydrolyzing 48h under the conditions of enzyme concentration 10FPU/g, pH4.8,50 DEG C, 150rpm, cellulase hydrolysis rate is 88.2%, is not added about
Add 5 times of tetrahydrofuran processing residue enzymatic hydrolyzation.
Embodiment 3: high temperature liquid water/furfural system
Using poplar bits as raw material, it is crushed to 100-200 mesh, is launched in reaction under high pressure narrow-necked earthen jar, according to substrate matter
Amount concentration is furfural/water mixed liquid that 20% addition volumetric concentration is 50%, and closed reaction system is heated to 185~195 DEG C,
Speed of agitator is 500rpm, and being passed through sulfur dioxide and keeping reaction pressure is that 5Mpa hydrolyzes 50min, after reaction, is directly carried out
It is separated by solid-liquid separation, water layer is mainly sugared product, and furfural layer is mainly sugared catabolite and lignin degradation products, and water phase is through efficient liquid
Analysis of hplc is about not added with the 5 of furfural situation it is found that total xylose (including xylose and xylo-oligosaccharide) rate of recovery is 75.7%
Again (high temperature liquid water treatment conditions are identical).The furfural extraction in furfural layer and solid residue is arrived with low boiling point solvents such as acetone
In acetone, extract liquor carries out acetone and furfural separation by distillation, and obtained furfural circulation is for processing next time.Solid residue at
It is found that cellulose retention rate is 89.9%, cellulolytic enzyme is added in delignification rate 91.1%, solid phase residue for analysis
It is hydrolyzed, substrate mass concentration 10%, enzyme concentration 15FPU/g, pH4.8,50 DEG C, after hydrolyzing 48h under the conditions of 150rpm, fiber
Plain enzymatic hydrolyzation is 89.6%, is about not added with 7 times of furfural processing residue enzymatic hydrolyzation.
Above embodiments prove, substitute high temperature liquid water, Ke Yishi using biology base polar non-solute part
The high-efficiency pretreatment of existing wood fiber biomass, it is bad to compensate for low delignification rate in conventional high-temperature liquid water preprocessing process
Gesture reduces cellulase dosage, balances the contradictory relation of water consumption and pretreating effect, it is seen that the improved high-temperature liquid state
The economy of water pretreatment process is greatly improved.
Claims (4)
1. a kind of method of improved high temperature liquid water preprocessing lignocellulose biolobic material, which is characterized in that this method packet
It includes following steps: being that 100-200 mesh lignocellulose-like biomass is added in hydrolysis reactor by granularity, volumetric concentration, which is added, is
Biology base polar non-solute/water mixed liquid of 1%-70%, the biology base polar non-solute be selected from tetrahydrofuran,
Furfural；Substrate mass concentration is 10%~20%, stirs evenly, 5-60min is hydrolyzed at 160-200 DEG C, and product carries out after reaction
It is separated by solid-liquid separation, most of cellulose is retained in solid phase and partial lignin, obtained liquid phase carry out organic phase/aqueous phase separation again,
Hemicellulose derived carbohydrate rich in obtained water phase, organic phase recycle to obtain biology base polar non-solute；It will consolidate
Phase residue pH4.8 carries out cellulose hydrolyzation 48h, and every gram of substrate adds 5~15FPU of cellulase.
2. the method for high temperature liquid water preprocessing lignocellulose biolobic material according to claim 1, which is characterized in that
One of ammonia, carbon dioxide, sulfur dioxide, oxygen, air, nitrogen is passed through in the reactor to pressurize.
3. the method for high temperature liquid water preprocessing lignocellulose biolobic material according to claim 1, which is characterized in that
Recycle the reaction solution that obtained biology base polar non-solute is recycled as next round hydrolysis.
4. the method for high temperature liquid water preprocessing lignocellulose biolobic material according to claim 1, which is characterized in that
The lignocellulose-like biomass be selected from sweet sorghum slag, bagasse, poplar bits, corncob, corn stover, Wheat Straw, straw,
One of switchgrass, energy grass.
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| CN107385119A (en) * | 2017-08-28 | 2017-11-24 | 华南理工大学 | A kind of method that corncob rapidly and efficiently prepares xylose hydrolysis fluid |
| CN109874928B (en) * | 2019-01-16 | 2022-10-04 | 中国科学院广州能源研究所 | Hydrothermal depolymerization method for improving quality of silage raw materials |
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| CN101586136A (en) * | 2009-07-03 | 2009-11-25 | 中国科学院广州能源研究所 | Method of high-efficiency pretreatment on biomass |
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|---|
| co-solvent pretreatment reduces costly enzyme requirements for high sugar and ethanol yields from lignocellulosic biomass;Thanh Yen Nguyen et al;《ChemSusChem》;20151231(第8期);摘要，第1717页左栏第3段，右栏第1段 * |
| 基于纤维素溶剂的木质纤维素预处理方法的研究进展;王钱钱等;《造纸科学与技术》;20151231;第34卷(第5期);第15-26页 * |
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