CN105769373A - Automatic cerebral cortex cooling inactivation system compatible with optical imaging and electrophysiological recording - Google Patents
Automatic cerebral cortex cooling inactivation system compatible with optical imaging and electrophysiological recording Download PDFInfo
- Publication number
- CN105769373A CN105769373A CN201610195714.1A CN201610195714A CN105769373A CN 105769373 A CN105769373 A CN 105769373A CN 201610195714 A CN201610195714 A CN 201610195714A CN 105769373 A CN105769373 A CN 105769373A
- Authority
- CN
- China
- Prior art keywords
- cortex
- cooling tube
- chassis
- temperature
- titanium alloy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
Abstract
The invention discloses an automatic cerebral cortex cooling inactivation system compatible with optical imaging and electrophysiological recording. The system mainly comprises two parts, namely, an automatic temperature control circuit and a titanium alloy implanting device, wherein the automatic temperature control circuit is used for controlling and regulating the temperature of the system, and the titanium alloy implanting device is a main structure of the system. By the aid of an electronic and mechanical system, on one hand, a single-chip microcomputer is used for performing automatic constant-temperature control on the temperature of the cortex and communicates with a computer to record the temperature of the cortex in real time, so that an experimenter is not required to continuously and manually perform temperature compensation regulation in the experimental process, and damage to the cortex due to freezing caused by excessive low temperature due to misoperation is avoided; on the other hand, by the aid of the inactivation and imaging integrated titanium alloy implanting device adjustable in three dimensions, a micro cooling tube can be positioned in the target cortex accurately, and synchronous recording, observation and stimulation can be performed with microelectrode, cortex optical imaging, two-photon imaging, optogenetics and other technologies during inactivation.
Description
Technical field
The present invention relates to automatization's cortex cooling inactivating system of a kind of compatible optical imaging and electrophysiological recording, this technology is mainly used in animal nerve Physiology Experiment research.
Background technology
The work that the information transmission between the function of a certain cortex and cortex is studied by the method for cooling inactivation is utilized to start from early in the twentieth century (survey article introducing the development of cortex falling temperature technique referring to Brooks).The research of early stage has wrapped up the wire chamber of thin rubber membrane by placing an one side on cortex surface, makes the frozen water of 4 DEG C flow there through, plays the effect reducing cortex temperature.The cortex scope of the method inactivation is relatively big, but concrete deactivated area is wayward, and is not used to the cortical region in inactivation brain ditch.
20 century 70s, along with the development of semiconductor technology, occur in that the experimental technique utilizing semiconductor refrigerating original paper to carry out cortex cooling, by placing a fritter silver strip on cortex for conducting heat, silver strip opposite side and semiconductor chilling plate laminating, lower the temperature to cortex；The representative studies work that the method carries out is utilized to refer to.Owing to the shape of silver strip is easily according to the cutting of brain district shape and processing, it is possible to deactivated area is controlled within specific scope.But its scope of application is caused restriction by the new problem that semiconductor chilling plate introduces: the hot side of semiconductor chilling plate can release more heat transfer, typically require and carry out water-cooling, cause single unit system thickness relatively big, be unsuitable for being implanted on cortex for a long time under animal waking state and carry out chronic experiment；In addition the electric current flow through during cooling piece work is relatively big, brain district aside can carry out extracellular electrophysiological recording (signal only has μ V level) simultaneously and produce larger interference.
Salsbury and Horel adopts in nineteen eighty-three reported first the miniature cooling tube of implanted to carry out the experimental technique of cortex cooling, Lomber et al. has carried out systematic test in the cooling Inactivation Effect to the method in 1999, has write correlation technique article and specific embodiments and effect are introduced.The program is by target cortex place implantable miniature cooling tube, under the driving of water pump, making the cold ethanol flowing through dry ice alcohol bath flow through from miniature cooling tube, reduce the purpose of cortex temperature, and principle is shown in Fig. 1.
The advantage of the method is in that cooling tube can bend to plan as required and carry out the shape corresponding to cortical region of deactivation experiment, and can be implanted on cortex for a long time or not cause cortical lesions in brain ditch.Additionally due to be connected to the only water pipe at cortex place and the thermocouple probe line that electric current is very faint, electrophysiological recording is not interfered with.But the method needs the artificial reading according to thermometer that water pump carries out constantly adjusting the temperature that could maintain cortex, careless slightly easily cause the too low icing of temperature and damage cortex, operate complex, therefore and be widely applied very much.In addition miniature cooling tube and joint are fixed on skull by dental cement by the method, it is impossible to mutually compatible with the cortex optical imagery risen in recent years, double; two (many) photon imaging technology, it is impossible to realize carrying out deactivation experiment under imaging.
Summary of the invention
The present invention is on the basis of Salsbury and Lomber et al. method, by a set of electronics and mechanical system, utilize single-chip microcomputer that cortex temperature is carried out automatic constant-temperature control on the one hand, and with the temperature of computer communication real time record cortex, make experimenter need not constantly be performed manually by temperature-compensating in experimentation to regulate, it is to avoid misoperation causes the too low damage cortex that freezes of temperature；On the other hand by three dimension adjustable inactivation imaging integration titanium alloy implanting device, make miniature cooling tube can be positioned at target cortex position accurately, and the technology such as microelectrode, cortex optical imagery, two-photon imaging and light heredity can be utilized while inactivation to carry out synchronous recording observation and stimulation.
For achieving the above object, the technical solution used in the present invention is automatization's cortex cooling inactivating system of a kind of compatible optical imaging and electrophysiological recording, native system is broadly divided into automatic temp. controlling circuit and titanium alloy implanting device two parts, automatic temp. controlling circuit is in order to controlling and regulating system temperature, and titanium alloy implanting device is the agent structure of native system；The automatic temp. controlling circuit temperature measurement circuit by host computer, control single chip computer with based on operational amplifier forms (Fig. 2).Temperature measurement circuit utilizes operational amplifier to read the thermoelectric voltage of T-shaped thermocouple probe, is sent to control single chip computer by spi bus after AD conversion.Temperature measurement circuit uses lithium battery independently-powered, high-speed photoelectric coupler is used to isolate with the spi bus of microcontroller communication, and isolate circuit part entirety to need to carry out electromagnetic shielding, to ensure that temp measuring system will not introduce outer source noise and electrophysiological recording is produced interference.After control single chip computer reads brain temperature data by spi bus, according to the target temperature set with PWM (Pulse-widthmodulation, pulse width modulation) mode control the rotating speed of refrigeration pump, the cold ethanol of final control flows through the flow velocity of cooling tube, makes brain temperature stable near design temperature.The real time temperature of cerebral tissue is turned UART serial ports by control single chip computer by USB and is sent to host computer, and is recorded by host computer.The orders such as target temperature, the beginning of refrigeration work and the stopping freezing are turned UART serial ports by host computer by USB and are sent to single-chip microcomputer.Thermograph and system on host computer control to be completed by independently developed software.
Titanium alloy implanting device is made up of pellosil 9, chassis 1, slide block 4, cover plate 2, top cover 3, side cover 5, wiring tower 6 and wiring tower lid 7.Wherein, pellosil 9 inserts under cerebral dura mater by the shirt rim stretched out, and relies on intracranial pressure and cerebral dura mater inwall gluing, sealing and is fixed on skull and windows place, protection cerebral tissue, and provides transparent imaging window.Chassis 1 is fixed on skull by three titanium alloy nails 10, is used for supporting and fixing cooling tube 8 and other parts.Cooling tube 8 traverse from the hole of slide block 4, relative position can adjust, and after final position is determined, M2 jackscrew is fixed on slide block 4.Slide block 4 can slide up and down in the fluting on chassis 1, or left-right rotation angle, and for adjusting the cooling tube 8 position on cortex, position is fixed by the slot M1.4 jackscrew on side of chassis 1 after determining.Cover plate 2 is fixed on chassis 1 by the flat head screw of eight symmetrical M1.4, is used for transferring record or imaging system, the size of cover plate 2 threaded upper ends and specification carry out adaptation according to the needs of record and imaging system.Wiring tower 6 is fixed on skull by three titanium alloy nails, for connecting thermocouple probe line 11, the installing hole of the internal binding post 12 (OMEGASMTC-CO-S and SMTC-CU-S) being provided with for installing thermocouple of wiring tower 6, insert perforate after needing before using to be connected thermocouple with binding post 12, and seal from the bottom of wiring tower 6 with orthopaedics cement.Top cover 2 is by being threadably mounted on cover plate 2, and centre is provided with passage, and seals by screwing in M2 screw.Side cover 3 is fixed on chassis 1 by two M1.4 screws.Wiring tower lid 7 is by being threadably mounted on wiring tower 6.Top cover 2, side cover 3 and wiring tower lid 7 are used for protecting that cooling tube 8, pellosil 9, cortex and binding post 12 be not contaminated, corrosion and external force damage, open after needing with povidone iodine, the outside of this system to be carried out disinfection in experimentation.
Pellosil 9 is formed by transparent silica gel compacting.
Wiring tower 6 is processed by polyetheretherketonematerials materials, and other parts process by titanium alloy (Ti-6Al-4VELI).
Accompanying drawing explanation
Fig. 1 is cortex chiller schematic diagram.
Fig. 2 is the circuit theory diagrams that automatic temperature-adjusting controls system.
Fig. 3 is the structure chart of titanium alloy implanting device.
Fig. 4 .1 is the schematic diagram that titanium alloy implanting device is installed in operation.
Fig. 4 .2 is the schematic diagram that titanium alloy implanting device A-A section is installed in operation.
Fig. 5 is the implementation result figure of cortex cooling.
In figure: 1, chassis, 2, cover plate, 3, top cover, 4, slide block, 5, side cover, 6, wiring tower, 7, wiring tower lid, 8, cooling tube, 9, pellosil, 10, titanium alloy nail, 11, thermocouple probe line, 12, binding post.
Detailed description of the invention
Fig. 1 is cortex chiller schematic diagram.The cool principle that the present invention adopts is identical with Salsbury and Lomber et al., but on their basis, add automatic temperature-adjusting control system, and have changed the fixed form of cooling tube, use the titanium alloy implanting device fixing cooling tube compatible with optical imagery, electrophysiological recording.
The method according to Salsbury and Lomber et al. is needed before experiment; rustless steel lumbar puncture needle (or thin stainless steel tube) is bent to cooling tube 8 and T-shaped thermocouple probe (using OMEGATT-T-30-SLE to manufacture) is welded on cooling tube root (position relationship is shown in Fig. 3); after protecting with politef heat-shrink tube; cooling tube end is inserted in the hole of slide block; and inflow and inflow entrance are separated certain angle, it is prevented that slide block comes off.Thermocouple probe line 11 end loads onto the binding post 12 (constantan and copper both threads use OMEGASMTC-CO-S and SMTC-CU-S respectively) of same metal material, and load the hole of wiring tower from bottom after, hole is shut by (Fig. 3) with bone cement.
Before carrying out cortex cooling deactivation experiment, need animal is performed the operation, implant cooling tube and titanium alloy implanting device, specifically comprise the following steps that after animal is stably anaesthetized and is fixed on operating-table, carry out operation of opening cranium in target cortex position, on skull centered by target location, open diameter and be about the circular bone window of 20mm (the cortical region range size that bone window diameter can inactivate according to schedule is adjusted, accordingly, the diameter of implanting device also should adjust therewith).Then cutting off cerebral dura mater, expose and treat inactivation brain district, map out the position that cooling tube 8 is contained on cortex, on pellosil 9, cooling tube punctures a circular duck eye through place with No. 12 injection needles.By cooling tube 8 head traverse from pellosil 9, adjust position and the angle of cooling tube 8, and the shirt rim of pellosil 9 is inserted in the cerebral dura mater gap with cortex.Chassis 1 is placed on skull, careful for slide block 4 is inserted from the groove on chassis 1 simultaneously, adjust position and the angle of chassis 1, slide block 4, cooling tube 8 and pellosil 9, make cooling tube be positioned exactly at target cortex place to be inactivated.With titanium alloy nail 10 (2.7mm diameter), chassis 1 is fixed on skull, tightens the jackscrew on chassis 1 and slide block 4 after the position of slide block 4 and cooling tube 8 is carried out finally fine setting, cooling tube 8 is fixed, and with screw, cover plate 2 is fixed on chassis 1.Wiring tower 6 is fixed on the skull on side with titanium alloy nail 10 (2.7mm diameter) after fixing by chassis 1; bone cement is inserted in the gap of chassis 1 and skull and the upper and lower gap of slide block 4 place fluting and seal, and the thermocouple probe line 11 between cooling tube 8 to wiring tower 6 is protected.Finally screwing on top cover 3 and bleed screw, load onto side cover 5 and wiring tower lid 7, skin suture (makes skin be looped around around titanium alloy implanting device, it is impossible to open top cover, side cover and wiring tower lid during impact experiment), completes operation.Position relationship reference Fig. 4 .1-4.2 of all parts and skull, cortex after installation.
The implementation result of cortex cooling asks for an interview Fig. 5, before visible cooling near (the 1st minute) cooling tube a certain neuronic action potential (spike) in cortex to provide frequency significantly high, after cooling inactivation, (the 3rd minute) cell almost no longer produces action potential, and after temperature recovery, the discharge activities of (the 6th minute) cell is recovered.
Claims (4)
1. automatization's cortex cooling inactivating system of a compatible optical imaging and electrophysiological recording, it is characterized in that: native system is broadly divided into automatic temp. controlling circuit and titanium alloy implanting device two parts, automatic temp. controlling circuit is in order to controlling and regulating system temperature, and titanium alloy implanting device is the agent structure of native system；The automatic temp. controlling circuit temperature measurement circuit by host computer, control single chip computer with based on operational amplifier forms；Temperature measurement circuit utilizes operational amplifier to read the thermoelectric voltage of T-shaped thermocouple probe, is sent to control single chip computer by spi bus after AD conversion；Temperature measurement circuit uses lithium battery independently-powered, high-speed photoelectric coupler is used to isolate with the spi bus of microcontroller communication, and isolate circuit part entirety to need to carry out electromagnetic shielding, to ensure that temp measuring system will not introduce outer source noise and electrophysiological recording is produced interference；After control single chip computer reads brain temperature data by spi bus, controlling the rotating speed of refrigeration pump in the way of PWM according to the target temperature set, the cold ethanol of final control flows through the flow velocity of cooling tube, makes brain temperature stable near design temperature；The real time temperature of cerebral tissue is turned UART serial ports by control single chip computer by USB and is sent to host computer, and is recorded by host computer；The orders such as target temperature, the beginning of refrigeration work and the stopping freezing are turned UART serial ports by host computer by USB and are sent to single-chip microcomputer；Thermograph and system on host computer control to be completed by independently developed software；
Titanium alloy implanting device is made up of pellosil (9), chassis (1), slide block (4), cover plate (2), top cover (3), side cover (5), wiring tower (6) and wiring tower lid (7)；Wherein, pellosil (9) inserts under cerebral dura mater by the shirt rim stretched out, relies on intracranial pressure and cerebral dura mater inwall gluing, sealing and is fixed on skull and windows place, protection cerebral tissue, and provides transparent imaging window；Chassis (1) is fixed on skull by three titanium alloy nails (10), is used for supporting and fixing cooling tube (8) and other parts；Cooling tube (8) traverse from the hole of slide block (4), relative position can adjust, and after final position is determined, M2 jackscrew is fixed on slide block (4)；Slide block (4) can slide up and down in the fluting of chassis (1), or left-right rotation angle, for adjusting the cooling tube (8) position on cortex, position is fixed by the M1.4 jackscrew on chassis (1) fluting side after determining；Cover plate (2) is fixed on chassis (1) by the flat head screw of eight symmetrical M1.4, record for transferring or imaging system, the size of cover plate (2) threaded upper ends and specification carry out adaptation according to the needs of record and imaging system；Wiring tower (6) is fixed on skull by three titanium alloy nails, for connecting thermocouple probe line (11), wiring tower (6) is internal is provided with binding post (the 12) (installing hole for installing thermocouple, insert perforate after needing before using to be connected thermocouple with binding post (12), and seal from the bottom of wiring tower (6) with orthopaedics cement；Top cover (2) is by being threadably mounted on cover plate (2), and centre is provided with passage, and seals by screwing in M2 screw；Side cover (3) is fixed on chassis (1) by two M1.4 screws；Wiring tower lid (7) is by being threadably mounted on wiring tower (6)；Top cover (2), side cover (3) and wiring tower lid (7) are used for protecting that cooling tube (8), pellosil (9), cortex and binding post (12) be not contaminated, corrosion and external force damage, open after needing with povidone iodine, the outside of this system to be carried out disinfection in experimentation.
2. automatization's cortex cooling inactivating system of a kind of compatible optical imaging according to claim 1 and electrophysiological recording, it is characterised in that: pellosil (9) is formed by transparent silica gel compacting.
3. automatization's cortex cooling inactivating system of a kind of compatible optical imaging according to claim 1 and electrophysiological recording, it is characterised in that: wiring tower (6) is processed by polyetheretherketonematerials materials.
4. utilize the implementation that the system described in claim 1 carries out; it is characterized in that: before experiment; rustless steel lumbar puncture needle or thin stainless steel tube are bent to cooling tube (8) and T-shaped thermocouple probe is welded on cooling tube root; after protecting with politef heat-shrink tube; cooling tube end is inserted in the hole of slide block; and inflow and inflow entrance are separated certain angle, it is prevented that slide block comes off；Thermocouple probe line (11) end loads onto the binding post (12) of same metal material, is shut by hole with bone cement load the hole of wiring tower from bottom after；
Before carrying out cortex cooling deactivation experiment, need animal is performed the operation, implant cooling tube and titanium alloy implanting device, specifically comprise the following steps that after animal is stably anaesthetized and is fixed on operating-table, operation of opening cranium is carried out in target cortex position, on skull centered by target location, open diameter and be about the circular bone window of 20mm；Then cut off cerebral dura mater, expose and treat inactivation brain district, map out the position that cooling tube (8) is contained on cortex, puncture a circular duck eye through place with (12) number injection needle at the upper cooling tube of pellosil (9)；By the traverse from pellosil (9) of cooling tube (8) head, adjust position and the angle of cooling tube (8), and the shirt rim of pellosil (9) is inserted in the cerebral dura mater gap with cortex；Chassis (1) is placed on skull, careful for slide block (4) is inserted from the groove of chassis (1) simultaneously, adjust position and the angle of chassis (1), slide block (4), cooling tube (8) and pellosil (9), make cooling tube be positioned exactly at target cortex place to be inactivated；With titanium alloy nail (10), chassis (1) is fixed on skull, the jackscrew on chassis (1) and slide block (4) is tightened after the position of slide block (4) and cooling tube (8) is carried out finally fine setting, cooling tube (8) is fixed, and with screw, cover plate (2) is fixed on chassis (1)；Wiring tower (6) is fixed on the skull on side with titanium alloy nail (10) after fixing by chassis (1); bone cement is inserted in the gap of chassis (1) and skull and the upper and lower gap of slide block (4) place fluting and seal, and the thermocouple probe line (11) between cooling tube (8) to wiring tower (6) is protected；Finally screw on top cover (3) and bleed screw, load onto side cover (5) and wiring tower lid (7), skin suture, complete operation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610195714.1A CN105769373B (en) | 2016-03-30 | 2016-03-30 | A kind of compatible optical imaging and the automation cortex cooling inactivating system of electrophysiological recording |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610195714.1A CN105769373B (en) | 2016-03-30 | 2016-03-30 | A kind of compatible optical imaging and the automation cortex cooling inactivating system of electrophysiological recording |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105769373A true CN105769373A (en) | 2016-07-20 |
| CN105769373B CN105769373B (en) | 2018-06-19 |
Family
ID=56395450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610195714.1A Expired - Fee Related CN105769373B (en) | 2016-03-30 | 2016-03-30 | A kind of compatible optical imaging and the automation cortex cooling inactivating system of electrophysiological recording |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105769373B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113712575A (en) * | 2021-07-16 | 2021-11-30 | 清华大学 | Whole brain multi-modal neural activity detection photoelectric brain-computer interface system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103006344A (en) * | 2012-12-05 | 2013-04-03 | 山东大学 | Semiconductor refrigerating frostbite model instrument |
| CN103501719A (en) * | 2011-03-02 | 2014-01-08 | 远藤护理有限公司 | Cryogenic medical system |
| WO2014039925A2 (en) * | 2012-09-07 | 2014-03-13 | Yale University | Brian cooling system |
| CN103800116A (en) * | 2012-11-07 | 2014-05-21 | 赵银香 | Medical human body cooling device controlled by single-chip microcomputer |
| CN104490372A (en) * | 2014-12-30 | 2015-04-08 | 续嘉 | Cooling device |
-
2016
- 2016-03-30 CN CN201610195714.1A patent/CN105769373B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103501719A (en) * | 2011-03-02 | 2014-01-08 | 远藤护理有限公司 | Cryogenic medical system |
| WO2014039925A2 (en) * | 2012-09-07 | 2014-03-13 | Yale University | Brian cooling system |
| CN103800116A (en) * | 2012-11-07 | 2014-05-21 | 赵银香 | Medical human body cooling device controlled by single-chip microcomputer |
| CN103006344A (en) * | 2012-12-05 | 2013-04-03 | 山东大学 | Semiconductor refrigerating frostbite model instrument |
| CN104490372A (en) * | 2014-12-30 | 2015-04-08 | 续嘉 | Cooling device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113712575A (en) * | 2021-07-16 | 2021-11-30 | 清华大学 | Whole brain multi-modal neural activity detection photoelectric brain-computer interface system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105769373B (en) | 2018-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7917222B1 (en) | Cerebral or organ interface system | |
| Muralidharan | External ventricular drains: Management and complications | |
| Erber et al. | Localization of short‐term memory in the brain of the bee, Apis mellifera | |
| US7177678B1 (en) | Bi-directional cerebral interface system | |
| DE69921449D1 (en) | METHOD FOR TREATING BRAIN DISEASES BY MEANS OF CONTROLLED HEAT SUPPLY | |
| CN107874896A (en) | Method and apparatus for low-temperature treatment skin histology | |
| US8454701B2 (en) | Surgical method for implanting a housing-interface assembly in a bone wall | |
| WO2009020862A3 (en) | Vestibular stimulation apparatus and associated methods of use | |
| WO2016165634A1 (en) | Cold and hot treatment device for melanoma | |
| TW290460B (en) | Perfusion hyperthermia treatment system and method | |
| Tai et al. | Temperature prediction in high speed bone grinding using motor PWM signal | |
| CN105769373A (en) | Automatic cerebral cortex cooling inactivation system compatible with optical imaging and electrophysiological recording | |
| CN1868415A (en) | Cold circulation microwave tumor therapeutic apparatus | |
| JP2021532873A (en) | Methods, devices, and systems to improve skin properties | |
| US20060015160A1 (en) | Method and apparatus for cooling spinal cord and fluid | |
| Anatychuk et al. | Thermoelectric device for temperature and heat flux density measurement ALTEC-10008 | |
| Wong et al. | Critical temperature transitions in laser-mediated cartilage reshaping | |
| CN1973788A (en) | Automatic brain tumor heat treating apparatus | |
| Zacarian | How accurate is temperature monitoring in cryosurgery and is there an alternative? | |
| CN103071246B (en) | The neuroactive prosthetic device of near infrared light | |
| Kastek et al. | Intraoperative application of thermal camera for the assessment of during surgical resection or biopsy of human’s brain tumors | |
| Caversaccio et al. | Cholesterol granuloma of the petrous apex: benefit of computer-aided surgery | |
| CN1903149A (en) | Method for lowering temp. and equipment therefor | |
| CN215534974U (en) | Thermosetting electrode and thermosetting system with same | |
| CN208625903U (en) | A kind of non-intrusion type head fixing device for primate test of eye movement |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180619 Termination date: 20200330 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |