CN105766629A - Method for fast propagation of Bhesasinensis seedlings through micro cuttage - Google Patents
Method for fast propagation of Bhesasinensis seedlings through micro cuttage Download PDFInfo
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- CN105766629A CN105766629A CN201410770956.XA CN201410770956A CN105766629A CN 105766629 A CN105766629 A CN 105766629A CN 201410770956 A CN201410770956 A CN 201410770956A CN 105766629 A CN105766629 A CN 105766629A
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Abstract
The invention discloses a method for fast propagation of Bhesasinensis seedlings through micro cuttage. The method comprises the following steps: inoculating Bhesasinensis seeds to a germination culture medium, and carrying out culture until the Bhesasinensis seeds grow to seedlings; cutting the obtained seedlings into stem segments, inserting the stem segments into a micro-cuttage culture medium, then carrying out culture until lateral buds grow out, cutting the lateral buds into stem segments, then inserting the stem segments into a fresh micro-cuttage culture medium, and carrying out culture; and cutting the obtained lateral buds into stem segments, then inoculating the stem segments to a rooting culture medium, and carrying out culture until the stem segments root so as to obtain Bhesasinensis seedlings. The method provided by the invention has the advantages of easy and practicable process, simple operation and fast propagation speed, solves the problems of less seed number, low germination rate and slow propagation speed in seedling propagation by utilizing the Bhesasinensis seeds, realizes large-scale and fast propagation of the Bhesasinensis seedlings in long term, can rapidly and continuously obtain Bhesasinensis test-tube seedlings in large quantity, and has strong practicability.
Description
Technical field
The invention belongs to agricultural biological technical field, be specially a kind of method utilizing stem section micro-cuttage Fast-propagation bhesa sinensis seedling.
Background technology
Bhesa sinensis (Bhesasinensis) belongs to Celastraceae bhesa sinensis platymiscium, half aiphyllium, only finds 3 strain bearing trees and 7 strain treelets in Guangxi coast, be China almost disappear endemic species, endangered species, first-grade state protection plant.China is this one only.The bhesa sinensis that Guangxi finds is the kind that the distribution of this genus is the most northern.Study of China plants of seed plant flora is geographical and tropical relationship has important scientific value.Bhesa sinensis florescence 7～JIUYUE, 3～April of next year, fruit maturation, was difficult to sexual propagation. without update step in the succession of community, it is badly in need of artificial propagation at present.
The traditional propagation method of bhesa sinensis is seminal propagation, and the method there is the problem that (1) seed resource is in short supply, batch capture difficulty；(2) percentage of seedgermination is low, only reaches about 50%.
Recently, cottage propagation becomes the research emphasis of bhesa sinensis sapling multiplication.The existing problem of cottage propagation is: (1) cottage propagation survival rate is low；(2) cutting resource scarcity, batch breeds difficulty.These problems seriously hinder the large-scale production of bhesa sinensis seedling, thus cannot meet the demand recovering afforestation.For that purpose it is necessary to foundation energy is a large amount of, the production technology system of fast-propagation bhesa sinensis seedling.
Summary of the invention
A kind of method that it is an object of the invention to provide micro-cuttage Fast-propagation bhesa sinensis seedling, is that one utilizes stem section sprouting of lateral bud and the fireballing feature of elongation in test tube, it is achieved that the purpose of bhesa sinensis seedling fast-propagation.
The present invention utilizes a small amount of seed to obtain aseptic seedling, with aseptic seedling stem sections for outer implant, promotes that lateral bud is constantly sprouted by test tube micro-cuttage and carries out fast breeding, it is achieved bhesa sinensis seedling vast propagation.
The technical solution adopted in the present invention: a kind of method of micro-cuttage Fast-propagation bhesa sinensis seedling, including the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process.
1, the acquisition process of aseptic seedling
Choose the ripe seed of bhesa sinensis sterilized after, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine, α-naphthaleneacetic acid.
2, stem section micro-cuttage process
Seedling step 1 obtained is cut into stem section, is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to and grows lateral bud, then lateral bud is cut into stem section, is then downwardly in fresh micro-cuttage culture medium by its base portion and cultivates.Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine, heteroauxing.
3, stem section rooting process
Lateral bud step 2 obtained is cut into stem section, and is inoculated on root media, and first light culture carries out illumination cultivation again, and taking root to stem section to obtain bhesa sinensis seedling.Described root media is with 1/2MS culture medium for minimal medium additional IAA, IBA.
Present invention process is simple; simple to operate; growth rate is fast; solve and utilize the problem that in bhesa sinensis seminal propagation seedling, seed amount is few, germination percentage is low, reproduction speed is slow; achieve the long-term extensive fast breeding of bhesa sinensis seedling, can in a large number, quickly, continue to obtain high-quality bhesa sinensis test tube Seedling, practical; efficient method is provided, for protecting wild bhesa sinensis kind matter to open a practicable approach for bhesa sinensis vast propagation propagation.
Detailed description of the invention
The invention will be further described below.
The method utilizing test tube micro-cuttage Fast-propagation bhesa sinensis seedling provided by the present invention, including the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process.
1), the acquisition process of aseptic seedling
3, April choose the ripe complete seed of bhesa sinensis sterilized after, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, cultivation temperature degree is 25～28 DEG C, and intensity of illumination is 15～20 μm of ol m-2·s-1, light application time is 10h d-1, cultivate 20～30 days；Described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 0.5mg L-1, α-naphthaleneacetic acid 0.1mg L-1。
2), stem section micro-cuttage process
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature degree is 25～28 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 10h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 1.5.0mg L-1, heteroauxing 0.1mg L-1。
3), stem section rooting process
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3～4 days again, and taking root to stem section to obtain bhesa sinensis seedling.Described root media is with 1/2MS culture medium for minimal medium additional IAA1.0mg L-1、IBA0.5mg·L-1。
Claims (1)
1. the method for a micro-cuttage Fast-propagation bhesa sinensis seedling, it is characterised in that: include the acquisition process of aseptic seedling, stem section micro-cuttage process, stem section rooting process；
1), the acquisition process of aseptic seedling
Choose the ripe excellent seed of bhesa sinensis sterilized after, seed is inoculated into germination culture medium and carries out being cultured to and grow up to seedling, cultivation temperature is 25～28 DEG C, and intensity of illumination is 15～20 μm of ol m-2·s-1, light application time is 10h d-1, cultivate 20～30 days；Described germination culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 0.5mg L-1, α-naphthaleneacetic acid 0.1mg L-1；
2), stem section micro-cuttage process
Seedling step 1 obtained is cut into the stem section with 1～2 joint, it is downwardly in micro-cuttage culture medium by the base portion of stem section to carry out being cultured to growing lateral bud, lateral bud is cut into the stem section with 1～2 joint again, then its base portion is downwardly in fresh micro-cuttage culture medium and cultivates, cultivation temperature is 25～28 DEG C, and intensity of illumination is 30～40 μm of ol m-2·s-1, light application time is 10h d-1；Described micro-cuttage culture medium is with MS culture medium for minimal medium additional 6-benzyladenine 1.5mg L-1, heteroauxing 0.1mg L-1；
3), stem section rooting process
Lateral bud step 2 obtained is cut into the stem section with 1～2 joint, and is inoculated on root media, and first light culture carries out illumination cultivation 20～30 days in 3～4 days again, and taking root to stem section to obtain bhesa sinensis seedling；Described root media is with 1/2MS culture medium for minimal medium additional IAA1.0mg L-1、IBA0.5mg·L-1。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410770956.XA CN105766629A (en) | 2014-12-15 | 2014-12-15 | Method for fast propagation of Bhesasinensis seedlings through micro cuttage |
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| CN201410770956.XA CN105766629A (en) | 2014-12-15 | 2014-12-15 | Method for fast propagation of Bhesasinensis seedlings through micro cuttage |
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| CN105766629A true CN105766629A (en) | 2016-07-20 |
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| CN201410770956.XA Pending CN105766629A (en) | 2014-12-15 | 2014-12-15 | Method for fast propagation of Bhesasinensis seedlings through micro cuttage |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
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2014
- 2014-12-15 CN CN201410770956.XA patent/CN105766629A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
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Application publication date: 20160720 |
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| WD01 | Invention patent application deemed withdrawn after publication |