Background technique
In using multiple light courcess excitation fluorescence concentrating device, for the consistency for guaranteeing different laser excitation regions, usually need
To be calculated by stringent light path, the fine adjustment of μm rank, come guarantee different laser reach optical axis height at targets it is identical,
Spot center point is overlapped.It is existing to apply in the technology of sequenator, generally on the basis of a light source, directly project, in addition
Light source by reflecting mirror change optical path and reference light source and close beam and realize purpose.
It in the related art, include two light sources a: reference light source and one in multiple light courcess excitation fluorescence concentrating device
Non-referenced light source.The light that reference light source projects is emitted directly toward dichroscope, and transmits away, and the light that non-referenced light source projects passes through
Directive dichroscope after the reflection of reflecting mirror, and reflect away.Two light beams for transmiting and reflecting by dichroscope are closed one
It rises and forms the further directive system light path of light beam.When in concentrating device including more than two light sources, the number of reflecting mirror
It can accordingly increase, the light beam eventually formed is the synthesis of light emitted by multiple light sources.
This kind of structure can have the following problem:
1, to guarantee the collimation of reflecting mirror and dichroscope when two light sources, the light after capable of just making conjunction beam does not have differential seat angle
Different, this, which is adjusted, needs higher accuracy.And when light source increases to three, reflecting mirror becomes two, two reflecting mirrors with
Dichroscope should keeping parallelism.If light source is more, need the component of keeping parallelism degree also more, calculate tune-up amount it is big and
Complexity, system adjustment can be fairly cumbersome time-consuming.
2, with the increase of light source, the usage amount of eyeglass also increases, then optical path takes up space and can also greatly increase.
3, since light refractive index in glass is different, then after dichroscope light have the offset of micron dimension
(dichroscope thickness is generally 1mm).In order to ensure the consistency for closing beam, non-referenced light source need to be translated in micron dimension, especially
For multiple light courcess system, reference light source needs repeatedly to pass through dichroscope, if the wavelength and process of the light beam that reference light source issues
When the wavelength difference of the light beam of the non-referenced light source after reflection, then the offset at dichroscope is also different, optical path meeting
It becomes more complicated, not only needs accurate calculating, more again increase adjusting difficulty.
Summary of the invention
The present invention is directed to solve at least some of the technical problems in related technologies.For this purpose, the present invention mentions
A kind of multiple light courcess concentrating device is gone out, the tune-up difficulty of the multiple light courcess concentrating device and required precision reduce.
The invention also provides a kind of sequenator optical systems with above-mentioned multiple light courcess concentrating device.
Multiple light courcess concentrating device according to embodiments of the present invention, comprising: at least two light sources, at least two light sources issue
Light beam respectively downward and in parallel;At least one reflecting mirror, the number of the reflecting mirror are less than the number of the light source, and at least one
A reflecting mirror is correspondingly arranged on the injection direction of at least one light beam and each reflecting mirror is respectively relative to institute
Light beam is stated to be obliquely installed;At least one dichroscope, at least one described dichroscope are located at the institute of the not set reflecting mirror
It states on the injection direction of the light of light source, the light by dichroscope transmission and the light by dichroscope reflection
Line is projected from the lower section of the dichroscope;Coupled lens, the coupled lens are located at the lower section of the dichroscope and are located at
On injection direction by the light of the dichroscope.
Multiple light courcess concentrating device according to an embodiment of the present invention so that by dichroscope transmit and emit light beam into
It can complete to close beam by coupled lens before entering system light path, to reduce tune-up complexity, adjustment part is being entered into light
It is completed before road, reduces tune-up required precision, and optimize optical system space layout.
In addition, multiple light courcess concentrating device according to the above embodiment of the present invention can also have following additional technology special
Sign:
According to one embodiment of present invention, the reflecting mirror includes multiple, multiple mirror parallel settings and edge
The injection direction of the light beam is spaced apart.
According to one embodiment of present invention, the light source includes first light source and second light source, the dichroscope packet
One is included, the dichroscope is located at below the first light source, and the reflecting mirror includes one, and the reflecting mirror is located at described
Below second light source, the reflecting mirror and the dichroscope are arranged in parallel.
According to one embodiment of present invention, height is consistent in the horizontal direction with the dichroscope for the reflecting mirror.
It according to one embodiment of present invention, further include output optical fibre, the output optical fibre is located under the coupled lens
Side simultaneously exports light by the dichroscope.
Sequenator optical system according to an embodiment of the present invention, including multiple light courcess optically focused according to an embodiment of the present invention are set
It is standby.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
Multiple light courcess concentrating device 100 according to an embodiment of the present invention is described in detail with reference to the accompanying drawing.
Shown in referring to Figures 1 and 2, multiple light courcess concentrating device 100 according to an embodiment of the present invention includes: at least two light
Source, at least one reflecting mirror 3, at least one dichroscope 2 and coupled lens 4.
The light beam that at least two light sources issue is respectively downward and in parallel.The number of reflecting mirror 3 is less than the number of light source.Tool is extremely
A few reflecting mirror 3 is correspondingly arranged on the injection direction of at least one light beam, and each reflecting mirror 3 is respectively relative to corresponding light
Beam is obliquely installed.At least one dichroscope 2 is located on the injection direction of the light of the light source of not set reflecting mirror 3.By two
The light transmitted to Look mirror 2 and the light by the reflection of dichroscope 2 are projected from the lower section of dichroscope 2.Coupled lens 4 are located at
It the lower section of dichroscope 2 and is located across on the injection direction of light of dichroscope 2.
Multiple light courcess concentrating device 100 according to an embodiment of the present invention, by the way that coupled lens 4 are arranged, so that by dichroic
The light beam of the transmission of mirror 2 and reflection can be completed to close beam before entering system light path, reduce tune-up complexity, adjustment part is existed
It is completed before into optical path, reduces required precision;And optimize optical system space layout.
Thus, it is only necessary to guarantee the light beam energy directive coupled lens 4 completely that all light sources issue, not need to make each light source
The center of the light beam of injection is all overlapped, that is, the problem of without the concern for light shift, greatly reduces calculation amount and adjustment
Difficulty.
If multiple homochromy low-power lasers coupled together, will also there be sizable side to excitation light power is improved
It helps.Due to current application the LD laser of High Speed Modulation general power only in 100mw rank, but its excitation power efficiency of fluorescence
It is relatively weak to be difficult to detect, it is easy to mix with bias light or stray light, the glimmering of excitation can be correspondinglyd increase if improving laser power
Optical power not only increases the signal-to-noise ratio of whole system in this way, also can accordingly shorten the time for exposure, improve flux system.
Reflecting mirror 3 may include it is multiple, multiple reflecting mirrors 3 be arranged in parallel and along the injection direction of light beam be spaced apart.For example,
As shown in Figure 1, light source includes first light source 11 and second light source 12 in an example of the invention, first light source 11 is base
Quasi-optical source, second light source 12 are non-referenced light source.Reflecting mirror 3 includes one, and reflecting mirror 3 is located at the lower section of second light source 12.Two
It include one to Look mirror 2, dichroscope 2 is located at 11 lower section of first light source, and reflecting mirror 3 and dichroscope 2 are arranged in parallel.
The light beam that first light source 11 projects can be emitted directly toward dichroscope 2.Second light source 12 project light beam can directive it is anti-
Mirror 3 is penetrated, and is reflected on dichroscope 2 by reflecting mirror 3.Dichroscope 2 can carry out the light beam that second light source 12 projects
Reflection, and the light beam projected to first light source 11 transmits.Two light beams for reflecting and transmiting by dichroscope 2 can be with one
It rises and projects, and continue directive coupled lens 4, by latter directive system light path of the coupling of coupled lens 4.
As shown in Fig. 2, light source includes first light source 11, second light source 12 and third light source 13, on the basis of first light source 11
Light source, second light source 12 and third light source 13 are non-referenced light source.Reflecting mirror 3 includes two, and the first reflecting mirror 31 and second is anti-
Penetrate mirror 32.First reflecting mirror 31 is located at 12 lower section of second light source, and the second reflecting mirror 32 is located at 13 lower section of third light source.Dichroscope
2 include two, the first dichroscope 21 and the second dichroscope 22.First dichroscope 21 is located at 11 lower section of first light source, the
Two dichroscopes 22 are located at the lower section of the first dichroscope 21.
The light beam that first light source 11 projects can be emitted directly toward the first dichroscope 21.The light beam that second light source 12 projects can be penetrated
It is reflected on the first dichroscope 21 to the first reflecting mirror 31, and by the first reflecting mirror 31.First dichroscope 21 can be right
The light beam that second light source 12 projects is reflected, and the light beam projected to first light source 11 transmits, the two light beams can be with
Occur to close beam, and the second dichroscope of directive 22 together.The second reflecting mirror of light beam directive 32 that third light source 13 projects, and pass through
Second reflecting mirror 32 is reflected on the second dichroscope 22.Second dichroscope 22 can transmit combined beam light, and to
The reflected light of three light sources 13 is reflected, this final two light beams can close beam and project from the second dichroscope 22, and continue
Directive coupled lens 4, by latter directive optical system of the coupling of coupled lens 4.
As shown in Figure 1, reflecting mirror 3 is with dichroscope 2, height can be consistent in the horizontal direction.To guarantee by reflection
The light beam that mirror 3 reflects can be irradiated on dichroscope 2.It is understood that the above feelings for being only two and three with light source
Condition is described in detail.Light source is that multiple situations is similar, here, being not described in detail.
As depicted in figs. 1 and 2, multiple light courcess concentrating device 100 further includes output optical fibre 5.Output optical fibre 5 is located at coupled lens
4 lower sections simultaneously export light by dichroscope 2.Light beam after coupling by coupled lens 4 can enter output optical fibre 5
One end is simultaneously exported from the other end of output optical fibre 5.When 5 long enough of output optical fibre of selection, it may be implemented for light source to be placed on and appoint
It what is the need for the place wanted, or entire light source can be moved on to outside system, be not take up original optical path space completely.
It is understood that light channel structure described above is light source feelings of outgoing beam from the top down along the vertical direction
Shape, light channel structure be not limited in it is described above, for example, light source can also project from left to right in the horizontal direction.Specifically,
The corresponding position of each component and angular relationship should carry out appropriate adjustment according to the direction of light beam.
Sequenator optical system according to an embodiment of the present invention includes multiple light courcess concentrating device according to an embodiment of the present invention
100.Since multiple light courcess concentrating device 100 according to an embodiment of the present invention has above-mentioned beneficial technical effect, according to this
The sequenator optical system of inventive embodiments reduces tune-up complexity and precision, the space layout of optical system are more preferable.
For Traditional Space coupling scheme, the present invention is tried out in actual test platform, reduces many tune
The lens rack device for saving the coincidence of two kinds of exciting lights has just readily obtained swashing for the different wave length of coaxial output without calculating adjustment
It shines;The occupied space of laser reduces, whole instrument smallerization.
In addition, the existing small-power co-wavelength laser in multiple markets is coupled based on this thinking, can save
The cost of exploitation or purchase high power laser, obtains the exciting light of higher-wattage, exposure needed for being further reduced biochip
Between light time, the flux of sequenator is improved.
Other of multiple light courcess concentrating device according to an embodiment of the present invention and sequenator optical system constitute and operation pair
For those skilled in the art be it is known, be not described in detail herein.
Light source in the present invention can be laser light source, be also possible to common beam light source.
In the description of the present invention, it is to be understood that, term " center ", " length ", " width ", " thickness ", "upper",
The orientation of the instructions such as "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside" or position are closed
System is merely for convenience of description of the present invention and simplification of the description to be based on the orientation or positional relationship shown in the drawings, rather than indicates
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For limitation of the present invention.
In the description of the present invention, the meaning of " plurality " is two or more, unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral;It can be mechanical connect
It connects, is also possible to be electrically connected;It can be directly connected, can also can be in two elements indirectly connected through an intermediary
The interaction relationship of the connection in portion or two elements.It for the ordinary skill in the art, can be according to specific feelings
Condition understands the concrete meaning of above-mentioned term in the present invention.
In the description of this specification, the description meaning of reference term " one embodiment ", " some embodiments ", " example " etc.
Refer to that particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one reality of the invention
It applies in example or example.In the present specification, schematic expression of the above terms be necessarily directed to identical embodiment or
Example.Moreover, particular features, structures, materials, or characteristics described can in any one or more of the embodiments or examples with
Suitable mode combines.In addition, without conflicting with each other, those skilled in the art can will describe in this specification
Different embodiments or examples and the features of different embodiments or examples be combined.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.