CN105753250B - A kind of purification method of city domestic sewage - Google Patents

A kind of purification method of city domestic sewage Download PDF

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CN105753250B
CN105753250B CN201610156358.2A CN201610156358A CN105753250B CN 105753250 B CN105753250 B CN 105753250B CN 201610156358 A CN201610156358 A CN 201610156358A CN 105753250 B CN105753250 B CN 105753250B
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algae
sewage
bridge
mixed
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CN105753250A (en
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王培磊
吕慎金
刘笑天
李爱水
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Linyi Dongxu Food Co.,Ltd.
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Linyi University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

The invention discloses a kind of purification method of city domestic sewage, belongs to field of environment protection.This method principle is:The nitrogen in sanitary sewage, phosphorus are absorbed using the bridge grown in block faces curved algae Cymbella and shank algae Synedra, so as to achieve the purpose that to denitrogenate, phosphorus and other organic matters.This method has the advantages that energy saving, efficient, effluent quality is good, floor space is small, small investment, and processing cost is low, easily operated.After measured, in waste water 98% nitrogen and 96% phosphorus is can remove by handling.

Description

A kind of purification method of city domestic sewage
Technical field
The invention belongs to field of environment protection, more particularly to a kind of purification method of city domestic sewage.
Background technology
On the purification method of city domestic sewage, past Chen Jiandan discloses a kind of city domestic sewage treatment process (application number CN201510758947.3), the technique include precipitation, active sludge treatment, sterilize and etc..The invention is provided City domestic sewage treatment process, water is sprayed in the form of dry fog, then with ultraviolet disinfection;Dry fog particle diameter itself is smaller, purple Outside line can more comprehensively sterilize, its effect can meet completely《People's Republic of China's standards for drinking water quality》 GB5749-2006 requirements, but infrastructure investment is big, it is troublesome in poeration.
In addition, Wang Chunai discloses a kind of purifying treatment method (application number of city domestic sewage CN201410058396.5 the pre-treatment step that), including successively carries out, biological respinse step, filtration step, sterilisation step and Adsorption step.Pre-treatment step can remove sandstone and large-sized floating material, and the water source of stabilization is provided for follow-up processing;It is raw Thing reactions steps are realized the low-carbon of city domestic sewage, are efficiently removed, and have processing load height, high treating effect, without additional Organic and inorganic carbon source, oxygen demand and regurgitant volume are low;Filtration step further eliminates tiny particles content and suspension in sewage Thing;Sterilisation step is gone out except the hygienic hazards such as bacterium, virus biology included in sewage;Adsorption step, can efficiently remove Calcium ions and magnesium ions, fluorine ion and other heavy metal ion in sewage.Product water after purified treatment, pollutant are gone substantially Remove, water stabilization, water quality is better than country-level A standards.But this method takes up a large area, cost is higher, easily causes secondary pollution. In order to overcome the shortcomings of current technology, the present invention provides a kind of two kinds of freshwater benthic diatom purifying city sanitary sewages of utilization Method.Bridge curved algae Cymbella and shank algae Synedra, which are two kinds, can be attached to the tables such as stone, wooden unit, stub, large-scale water plant Look unfamiliar long freshwater benthic diatom.This method principle is:It will be lived using the curved algae of the bridge grown in block faces and shank algae dirty Nitrogen, phosphorus in water absorb, so as to achieve the purpose that to denitrogenate phosphorus and other organic matters;In other words, the curved algae of bridge, shank algae are by sewage In nitrogen, phosphorus be used as the nutritive salt of metabolism, then with precipitating reagent quick lime by the curved algae of bridge in algae solution and Shank algae is precipitated and separates, so as to reach removal purpose.This method have it is energy saving, efficient, effluent quality is good, floor space The features such as small, small investment, processing cost is low, easily operated.After measured, in waste water 98% nitrogen and 96% is can remove by handling Phosphorus.
The content of the invention
The object of the present invention is to provide a kind of purification method of city domestic sewage, this purpose is by following technical solution Realize:
A kind of mixed-culture medium of purification method for city domestic sewage, mixed-culture medium are the curved algae of bridge and shank algae Mixed-culture medium, the curved algae of the bridge and shank algae mixed-culture medium by following parts by weight composition:Pretreated life 12-18 parts of sewage living, 0.04-0.09 parts of calcium chloride, 0.2-0.7 parts of potassium silicate, 0.03-0.08 parts of magnesium sulfate, manganese sulfate 0.03- 0.08 part, 0.005-0.009 parts of zinc chloride, 0.0003-0.0005 parts of copper chloride, 0.02-0.07 parts of frerrous chloride, sodium molybdate 0.0001-0.0003 parts, 0.0004-0.0007 parts of b diammonium edta sodium, 0.004-0.008 parts of cobalt chloride, vanadic anhydride 0.006-0.008 parts, 0.0004-0.0008 parts of vitamin B1,0.0007-0.0009 parts of vitamin B12, vitamin H0.0004-0.0007 parts, 0.002-0.008 parts of folic acid, 0.007-0.009 parts of inositol, 3-6 parts of composite Chinese herbal liquid, indoles second It is 0.002-0.007 parts sour, 1000 parts of distilled water;Wherein composite Chinese herbal liquid and preparation method thereof:Glutinous rehmannia 3-7 is taken by ratio of weight and the number of copies Part, 4-9 parts of radix bupleuri, 1-3 parts of matrimony vine, 8-12 parts of folium artemisiae argyi, 3-6 parts of purple perilla, 5-7 parts of the bark of eucommia, 300 parts of pure water, heating, boils, Then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
Preferably, the pretreated sanitary sewage pre-treatment step is as follows:First with the silk cover filtering sewage of 60 mesh, remove Remove floating material, glass bits, hair, silt, leaf, polybag larger solid content in equal volume, after sewage is introduced it is 30 meters long, wide In 20 meters, deeply 0.8 meter of cement aeration tank, pond is in east-west, to receive more sunlights;The depth of water is maintained at 60 lis Rice;The amount that 60 grams of quick limes are added by every cubic metre of sewage is uniformly splashed quick lime, is added every 30 minutes, then by every cubic metre of sewage Enter 45 grams of amount full pool spilling head alum, be then fully aerated, stirred 60 minutes with air compressor machine, make lime and alum fully dissolve, Mix and fully chemically reacted with dirty Organic substance in water, when then natural sedimentation, fermentation, exposure 48 are small, skim water surface bubble The floating materials such as foam, do not stir bottom of pond, take sewage at the middle and upper levels, are fitted into lighttight black plastic bucket, are sealed, spare.
The purification method of the mixed-culture medium processing sanitary sewage of the purification method for city domestic sewage, step Rapid 1:The curved algae of bridge and shank algae inoculation method:The common plastics bucket for taking a volume to be 40 liters, adds the curved algae of above-mentioned bridge and shank 25 liters of algae mixed-culture medium, then takes in exponential phase of growth, clean, pollution-free, the curved algae algae of eugonic bridge and shank Algae algae is inoculated with respectively, and inoculum density is bridge 4.5 ten thousand cells/mls of curved algae, and 3.5 ten thousand cells/mls of shank algae, gently shake It is even, bung hole is muffled with one block of plastic film, avoids polluting, it is stand-by;
Step 2:The curved algae of bridge and shank algae mixed culturing method:One 25 liters of vitreous distilled water bottle is taken, is washed, Distilled water bottle after washing is sterilized 20 minutes using disinfectant again, and the disinfectant is Chinese herbal medicine composite disinfectant;It is described Chinese herbal medicine composite disinfectant constituent (ratio of weight and number):Red 2-4 parts of dish of the back of the body, 6-9 parts of cordate houttuynia, 5-8 parts of radix bupleuri, Longtube Ground Ivy Herb 7-12 parts, 10-15 parts of wrinkled giant hyssop, 7-12 parts of rhizoma atractylodis, 8-15 parts of the fruit of summer cypress, 5-7 parts of Chinese violet, ophiopogon 3-6 part, Radix Isatidis 0.5- 0.9 part, 10-14 parts of the tuber of stemona, 5-8 parts of Herba Andrographitis, 2-6 parts of dandelion, 1-3 parts of Phellinus;Then from the distilled water bottle bottle after disinfection Mouth puts small stone block into the 2/3 of distilled water bottle volume, and every piece of stone weight is 100-120 grams, is then added from bottleneck described The algae solution being inoculated with untill just stone block is not come;Bottleneck is sealed with 2 layers of clean newspaper and rubber band, this is distilled Water bottle puts the culture of microdisk electrode room, culturing room's air-conditioning temperature control into together with algae solution therein and stone block, and fluorescent tube provides illumination, Carry out regulating illumination intensity to open the quantity of fluorescent tube;Co-culture 8 days, intensity of illumination 6000lux, 25 DEG C of temperature, light are set Cycle is set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when illumination, 8 is dark when small, moves in circles;Arrive 8th day end, stops culture, the tap of algae solution from Distilled water bucket bottom is put into the plastic barrel that a volume is 30 liters, this When major part frustule be attached to gravelstone head surface, small part is in algae solution;
Step 3:Microalgae and water body separation method:Added every liter of algae solution is pressed in the algae solution in described 30 liters of plastic barrel 5 grams of amount adds quick lime, is gently mixed, and when standing 8 is small, frustule and lime bits sink to bottom at this time, are inhaled with siphon pipe Supernatant is taken, the clean water after as handling abandons bottom sediment, and purification is completed.
Purification method for the mixed-culture medium processing sanitary sewage of the purification method of city domestic sewage, it is preferable that The second wheel sewage disposal is carried out, the second wheel sewage treatment step is as follows:When carrying out the second wheel sewage disposal, because stone surface is attached The curved algae of substantial amounts of bridge and shank algae, without carrying out inoculation algae again, it is only necessary to by the curved algae of the above-mentioned bridge prepared and shank algae Mixed-culture medium be added to it is described untill bleeding off in the distilled water bottle of algae solution and just submerging stone, then with 2 layers of clean report Paper and rubber band seal bottleneck, put this Distilled water bucket into the culture of microdisk electrode room, culture together with algae solution therein and stone block Room air-conditioning temperature control, fluorescent tube provide illumination, carry out regulating illumination intensity to open the quantity of fluorescent tube, co-culture 8 days, set Intensity of illumination 6000lux, 25 DEG C of temperature, photoperiod are set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when Illumination, 8 is dark when small, moves in circles;To the 8th day end, stop culture, put the tap of algae solution from distilled water bottle bottom into one A volume is in 30 liters of plastic barrel, and so repeatedly, it is all to terminate to carry out 5 wheel cultures altogether, algae solution aging at this time, and in algae solution A large amount of miscellaneous bacterias, other microalgaes, protozoan etc. have been infiltrated, if it is desired to sewage is continued with, it is necessary to which stone is taken out from bottleneck, will Distilled water bottle and stone wash, and repeat above-mentioned all operations from step 1 to step 3.
Preferably, a kind of purification method of city domestic sewage can also be summarized as follows 5 steps:
The curved algae of step Bridge 1 and the curved algae of preparation bridge of shank algae mixed-culture medium and shank algae mixed-culture medium are by following weight The composition of number:12-18 parts of pretreated sanitary sewage, 0.04-0.09 parts of calcium chloride, 0.2-0.7 parts of potassium silicate, sulphur Sour magnesium 0.03-0.08 parts, 0.03-0.08 parts of manganese sulfate, 0.005-0.009 parts of zinc chloride, 0.0003-0.0005 parts of copper chloride, 0.02-0.07 parts of frerrous chloride, 0.0001-0.0003 parts of sodium molybdate, 0.0004-0.0007 parts of b diammonium edta sodium, chlorination 0.004-0.008 parts of cobalt, 0.006-0.008 parts of vanadic anhydride, 0.0004-0.0008 parts of vitamin B1, vitamin B12 0.0007-0.0009 parts, 0.0004-0.0007 parts of biotin, 0.002-0.008 parts of folic acid, 0.007-0.009 parts of inositol, 3-6 parts of composite Chinese herbal liquid, 0.002-0.007 parts of heteroauxin, 1000 parts of distilled water.Wherein composite Chinese herbal liquid preparation side Method:Take 3-7 parts of glutinous rehmannia by weight, 4-9 parts of radix bupleuri, 1-3 parts of matrimony vine, 8-12 parts of folium artemisiae argyi, 3-6 parts of purple perilla, 5-7 parts of the bark of eucommia is pure 300 parts of water purification, heating, boils, and then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
The physiological function of each component is as follows in nutrient solution:Sanitary sewage is mainly provided required for the curved algae of bridge and the growth of shank algae Nitrogen, phosphorus;Calcium chloride, potassium silicate, magnesium sulfate, manganese sulfate, zinc chloride, copper chloride, frerrous chloride, sodium molybdate, second diammonium tetrem Sour sodium, cobalt chloride, vanadic anhydride mainly provide microelements of calcium, silicon, magnesium, manganese, zinc, copper, iron, molybdenum, sodium, cobalt, vanadium;Vitamin B1, vitamin B12, biotin, folic acid, inositol mainly provide various vitamins;Composite Chinese herbal liquid is mainly used for killing and presses down Bacterium, virus and protozoan in algae solution processed;Heteroauxin is a kind of plant growth substance, and the curved algae of bridge and shank algae are given birth to With fine facilitation.
The curved algae of step Bridge 2 and the inoculation of shank algae take the common plastics bucket that a volume is 40 liters, and the bridge added described in step 1 is curved 25 liters of algae and shank algae mixed-culture medium, then take in exponential phase of growth, clean, pollution-free, the eugonic curved algae algae of bridge Kind and shank algae algae be inoculated with respectively, inoculum density is bridge 4.5 ten thousand cells/mls of curved algae, 3.5 ten thousand cell/millis of shank algae Rise, gently shake up, muffle bung hole with one block of plastic film, avoid polluting, it is stand-by;
Step 3 microdisk electrode takes one 25 liters of vitreous distilled water bottle, washes, from bottleneck put into small stone block to The 2/3 of distilled water bottle volume, every piece of stone weight is 100-120 grams, and the algae being inoculated with described in step 2 is then added from bottleneck Liquid is not untill coming stone block just;Bottleneck is sealed with 2 layers of clean newspaper and rubber band, by this distilled water bottle together with wherein Algae solution and stone block put into microdisk electrode room culture, culturing room's air-conditioning temperature control, fluorescent tube provide illumination, to open fluorescent tube Quantity carrys out regulating illumination intensity;Co-culture 8 days, intensity of illumination 6000lux is set, and 25 DEG C of temperature, the photoperiod is set to L16+ D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when illumination, 8 is dark when small, moves in circles;To the 8th day end, stop Culture, the tap of algae solution from distilled water bottle bottom is put into the plastic barrel that a volume is 30 liters, and most of algae is thin at this time Born of the same parents are attached to gravelstone head surface, and small part is in algae solution.
The amount that every liter of 5 grams of algae solution addition is pressed in algae solution described in step 3 is added raw stone by step 4 microalgae and water body separation Ash, is gently mixed, and when standing 8 is small, frustule and lime bits sink to bottom at this time, draws supernatant with siphon pipe, as locates Clean water after reason, abandons bottom sediment, and purification is completed.
Step 5 second takes turns sewage disposal when carrying out the second wheel sewage disposal, and because of stone surface, to be attached to substantial amounts of bridge curved Algae and shank algae, without carrying out inoculation algae again, it is only necessary to which the curved algae of bridge for preparing step 1 and shank algae mixed-culture medium add Untill being bled off described in step 3 in the distilled water bottle of algae solution and just submerge stone.Then with 2 layers of clean newspaper and rubber band Bottleneck is sealed, this distilled water bottle is put into microdisk electrode room together with algae solution therein and stone block and cultivates, culturing room's air-conditioning temperature control, Fluorescent tube provides illumination, carrys out regulating illumination intensity to open the quantity of fluorescent tube.Co-culture 8 days, intensity of illumination is set 6000lux, 25 DEG C of temperature, photoperiod are set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when illumination, 8 is small When it is dark, move in circles;To the 8th day end, stop culture, putting the tap of algae solution from distilled water bottle bottom into a volume is In 30 liters of plastic barrel.So repeatedly, it is all to terminate to carry out 5 wheel cultures altogether.Algae solution aging at this time, and infiltrated in algae solution big Miscellaneous bacteria, other microalgaes, protozoan etc. are measured, if it is desired to continuing with sewage, it is necessary to stone be taken out from bottleneck, the stone renewed Head, repeats all operations from step 1 to step 4.
Beneficial outcomes
This method principle is:It will be lived using the bridge grown in block faces curved algae Cymbella and shank algae Synedra Nitrogen in sewage, phosphorus absorb, so as to achieve the purpose that to denitrogenate, phosphorus and other organic matters.This method have it is energy saving, efficient, go out The advantages that water water quality is good, floor space is small, small investment, and processing cost is low, easily operated.After measured, give up by handling can remove 98% nitrogen and 96% phosphorus in water.
Brief description of the drawings
The curved algae of bridge is cultivated in Fig. 1 distilled water bottles and shank algae carrys out the schematic diagram of purifying city sanitary sewage.
Scheme medium and small stone block and be immersed in micro algae culturing liquid, bottle mouth position is sealed with 2 layers of clean newspaper and rubber band.
Embodiment
Further the technology is illustrated with reference to embodiment.
A kind of mixed-culture medium of purification method for city domestic sewage, mixed-culture medium are the curved algae of bridge and shank algae Mixed-culture medium, the curved algae of the bridge and shank algae mixed-culture medium by following parts by weight composition:Pretreated life 12-18 parts of sewage living, 0.04-0.09 parts of calcium chloride, 0.2-0.7 parts of potassium silicate, 0.03-0.08 parts of magnesium sulfate, manganese sulfate 0.03- 0.08 part, 0.005-0.009 parts of zinc chloride, 0.0003-0.0005 parts of copper chloride, 0.02-0.07 parts of frerrous chloride, sodium molybdate 0.0001-0.0003 parts, 0.0004-0.0007 parts of b diammonium edta sodium, 0.004-0.008 parts of cobalt chloride, vanadic anhydride 0.006-0.008 parts, 0.0004-0.0008 parts of vitamin B1,0.0007-0.0009 parts of vitamin B12, vitamin H0.0004-0.0007 parts, 0.002-0.008 parts of folic acid, 0.007-0.009 parts of inositol, 3-6 parts of composite Chinese herbal liquid, indoles second It is 0.002-0.007 parts sour, 1000 parts of distilled water;Wherein composite Chinese herbal liquid and preparation method thereof:Glutinous rehmannia 3-7 is taken by ratio of weight and the number of copies Part, 4-9 parts of radix bupleuri, 1-3 parts of matrimony vine, 8-12 parts of folium artemisiae argyi, 3-6 parts of purple perilla, 5-7 parts of the bark of eucommia, 300 parts of pure water, heating, boils, Then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
Preferably, the pretreated sanitary sewage pre-treatment step is as follows:First with the silk cover filtering sewage of 60 mesh, remove Remove floating material, glass bits, hair, silt, leaf, polybag larger solid content in equal volume, after sewage is introduced it is 30 meters long, wide In 20 meters, deeply 0.8 meter of cement aeration tank, pond is in east-west, to receive more sunlights;The depth of water is maintained at 60 lis Rice;The amount that 60 grams of quick limes are added by every cubic metre of sewage is uniformly splashed quick lime, is added every 30 minutes, then by every cubic metre of sewage Enter 45 grams of amount full pool spilling head alum, be then fully aerated, stirred 60 minutes with air compressor machine, make lime and alum fully dissolve, Mix and fully chemically reacted with dirty Organic substance in water, when then natural sedimentation, fermentation, exposure 48 are small, skim water surface bubble The floating materials such as foam, do not stir bottom of pond, take sewage at the middle and upper levels, are fitted into lighttight black plastic bucket, are sealed, spare.
The purification method of the mixed-culture medium processing sanitary sewage of the purification method for city domestic sewage, step Rapid 1:The curved algae of bridge and shank algae inoculation method:The common plastics bucket for taking a volume to be 40 liters, adds the curved algae of above-mentioned bridge and shank 25 liters of algae mixed-culture medium, then takes in exponential phase of growth, clean, pollution-free, the curved algae algae of eugonic bridge and shank Algae algae is inoculated with respectively, and inoculum density is bridge 4.5 ten thousand cells/mls of curved algae, and 3.5 ten thousand cells/mls of shank algae, gently shake It is even, bung hole is muffled with one block of plastic film, avoids polluting, it is stand-by;
Step 2:The curved algae of bridge and shank algae mixed culturing method:One 25 liters of vitreous distilled water bottle is taken, is washed, Distilled water bottle after washing is sterilized 20 minutes using disinfectant again, and the disinfectant is Chinese herbal medicine composite disinfectant;It is described Chinese herbal medicine composite disinfectant constituent (ratio of weight and number):Red 2-4 parts of dish of the back of the body, 6-9 parts of cordate houttuynia, 5-8 parts of radix bupleuri, Longtube Ground Ivy Herb 7-12 parts, 10-15 parts of wrinkled giant hyssop, 7-12 parts of rhizoma atractylodis, 8-15 parts of the fruit of summer cypress, 5-7 parts of Chinese violet, ophiopogon 3-6 part, Radix Isatidis 0.5- 0.9 part, 10-14 parts of the tuber of stemona, 5-8 parts of Herba Andrographitis, 2-6 parts of dandelion, 1-3 parts of Phellinus;Then from the distilled water bottle bottle after disinfection Mouth puts small stone block into the 2/3 of distilled water bottle volume, and every piece of stone weight is 100-120 grams, is then added from bottleneck described The algae solution being inoculated with untill just stone block is not come;Bottleneck is sealed with 2 layers of clean newspaper and rubber band, this is distilled Water bottle puts the culture of microdisk electrode room, culturing room's air-conditioning temperature control into together with algae solution therein and stone block, and fluorescent tube provides illumination, Carry out regulating illumination intensity to open the quantity of fluorescent tube;Co-culture 8 days, intensity of illumination 6000lux, 25 DEG C of temperature, light are set Cycle is set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when illumination, 8 is dark when small, moves in circles;Arrive 8th day end, stops culture, the tap of algae solution from Distilled water bucket bottom is put into the plastic barrel that a volume is 30 liters, this When major part frustule be attached to gravelstone head surface, small part is in algae solution;
Step 3:Microalgae and water body separation method:Added every liter of algae solution is pressed in the algae solution in described 30 liters of plastic barrel 5 grams of amount adds quick lime, is gently mixed, and when standing 8 is small, frustule and lime bits sink to bottom at this time, are inhaled with siphon pipe Supernatant is taken, the clean water after as handling abandons bottom sediment, and purification is completed.
Purification method for the mixed-culture medium processing sanitary sewage of the purification method of city domestic sewage, it is preferable that The second wheel sewage disposal is carried out, the second wheel sewage treatment step is as follows:When carrying out the second wheel sewage disposal, because stone surface is attached The curved algae of substantial amounts of bridge and shank algae, without carrying out inoculation algae again, it is only necessary to by the curved algae of the above-mentioned bridge prepared and shank algae Mixed-culture medium be added to it is described untill bleeding off in the distilled water bottle of algae solution and just submerging stone, then with 2 layers of clean report Paper and rubber band seal bottleneck, put this Distilled water bucket into the culture of microdisk electrode room, culture together with algae solution therein and stone block Room air-conditioning temperature control, fluorescent tube provide illumination, carry out regulating illumination intensity to open the quantity of fluorescent tube, co-culture 8 days, set Intensity of illumination 6000lux, 25 DEG C of temperature, photoperiod are set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when Illumination, 8 is dark when small, moves in circles;To the 8th day end, stop culture, put the tap of algae solution from distilled water bottle bottom into one A volume is in 30 liters of plastic barrel, and so repeatedly, it is all to terminate to carry out 5 wheel cultures altogether, algae solution aging at this time, and in algae solution A large amount of miscellaneous bacterias, other microalgaes, protozoan etc. have been infiltrated, if it is desired to sewage is continued with, it is necessary to which stone is taken out from bottleneck, will Distilled water bottle and stone wash, and repeat above-mentioned all operations from step 1 to step 3.
The curved algae of application examples Bridge 1 and shank algae mixed-culture medium by following parts by weight composition:Pretreated life 13 parts of sewage living, 0.05 part of calcium chloride, 0.2 part of potassium silicate, 0.03 part of magnesium sulfate, 0.04 part of manganese sulfate, 0.006 part of zinc chloride, 0.0003 part of copper chloride, 0.03 part of frerrous chloride, 0.0001 part of sodium molybdate, 0.0004 part of b diammonium edta sodium, cobalt chloride 0.004 part, 0.006 part of vanadic anhydride, 0.0004 part of vitamin B1,0.0007 part of vitamin B12, biotin 0.0004 Part, 0.003 part of folic acid, 0.007 part of inositol, 3 parts of composite Chinese herbal liquid, 0.003 part of heteroauxin, 1000 parts of distilled water.Wherein Composite Chinese herbal liquid and preparation method thereof:4 parts of glutinous rehmannia, 6 parts of radix bupleuri, 1 part of matrimony vine, 9 parts of folium artemisiae argyi, 4 parts of purple perilla, the bark of eucommia 5 are taken by weight Part, 300 parts of pure water, heating, boils, and then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
The curved algae of application examples Bridge 2 and shank algae mixed-culture medium by following parts by weight composition:Pretreated life 14 parts of sewage living, 0.07 part of calcium chloride, 0.5 part of potassium silicate, 0.04 part of magnesium sulfate, 0.04 part of manganese sulfate, 0.008 part of zinc chloride, 0.0005 part of copper chloride, 0.04 part of frerrous chloride, 0.0002 part of sodium molybdate, 0.0005 part of b diammonium edta sodium, cobalt chloride 0.006 part, 0.007 part of vanadic anhydride, 0.0005 part of vitamin B1,0.0008 part of vitamin B12, biotin 0.0006 Part, 0.005 part of folic acid, 0.008 part of inositol, 4 parts of composite Chinese herbal liquid, 0.005 part of heteroauxin, 1000 parts of distilled water.Wherein Composite Chinese herbal liquid and preparation method thereof:5 parts of glutinous rehmannia, 6 parts of radix bupleuri, 2 parts of matrimony vine, 10 parts of folium artemisiae argyi, 5 parts of purple perilla, the bark of eucommia 6 are taken by weight Part, 300 parts of pure water, heating, boils, and then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
The curved algae of 3 bridge of application examples and shank algae mixed-culture medium by following parts by weight composition:Pretreated life 17 parts of sewage living, 0.08 part of calcium chloride, 0.7 part of potassium silicate, 0.08 part of magnesium sulfate, 0.07 part of manganese sulfate, 0.008 part of zinc chloride, 0.0005 part of copper chloride, 0.06 part of frerrous chloride, 0.0003 part of sodium molybdate, 0.0007 part of b diammonium edta sodium, cobalt chloride 0.008 part, 0.008 part of vanadic anhydride, 0.0007 part of vitamin B1,0.0009 part of vitamin B12, biotin 0.0007 Part, 0.007 part of folic acid, 0.008 part of inositol, 6 parts of composite Chinese herbal liquid, 0.007 part of heteroauxin, 1000 parts of distilled water.Wherein Composite Chinese herbal liquid and preparation method thereof:7 parts of glutinous rehmannia, 8 parts of radix bupleuri, 3 parts of matrimony vine, 11 parts of folium artemisiae argyi, 6 parts of purple perilla, the bark of eucommia 7 are taken by weight Part, 300 parts of pure water, heating, boils, and then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
Sanitary sewage preprocess method sanitary sewage used is used to before supporting algae need to be pre-processed, and method is:First with 60 Purpose silk cover filtering sewage, removes floating material, glass bits, hair, silt, leaf, polybag larger solid content in equal volume, after Sewage is introduced into 30 meters long, 20 meters wide, 0.8 meter deep cement aeration tank, pond is in east-west, to receive more sunlight Irradiation;The depth of water is maintained at 60 centimetres;The amount that 60 grams of quick limes are added by every cubic metre of sewage is uniformly splashed quick lime, every 30 points Clock, then the amount full pool spilling head alum by every cubic metre of 45 grams of sewage addition, are then fully aerated with air compressor machine, stirred 60 minutes, Lime and alum are fully dissolved, are mixed and fully be chemically reacted with dirty Organic substance in water, then natural sedimentation, fermentation, Expose 48 it is small when, skim the floating materials such as water surface foam, do not stir bottom of pond, take sewage at the middle and upper levels, load the modeling of lighttight black In charging basket, it is sealed, it is spare;
Stone used should be cleaned in advance, be placed in distilled water and heat, and boil 5 minutes, and disinfection, uses after cooling;
Calcium chloride used, potassium silicate, magnesium sulfate, manganese sulfate, zinc chloride, copper chloride, frerrous chloride, sodium molybdate, second diammonium Tetraacethyl sodium, cobalt chloride, vanadic anhydride, vitamin B1, vitamin B12, biotin, folic acid, inositol, heteroauxin chemistry Medicine purity is both needed to the pure rank of chemistry;
The curved algae of bridge of the inoculation and shank algae should be purified in advance, and (this area conventional method, carries as long as can realize It is pure, such as Guo Shi extraction methods), algae is purebred and be in eugonic exponential phase of growth during ensuring inoculation;
The distilled water bottle non-refractory can not heat retort sterilization on stove or directly be scalded with boiling water when scrubbing, it should Washed with 8% dilute sulfuric acid or 15% dilute hydrochloric acid, then with distilled water rinse 3-5 times.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that:It still may be used To modify to the technical solution described in previous embodiment, or equivalent substitution is carried out to which part technical characteristic;And These modifications are replaced, and the essence of appropriate technical solution is departed from the spirit and model of technical solution of the embodiment of the present invention Enclose.

Claims (3)

1. the mixed-culture medium of a kind of purification method for city domestic sewage, it is characterised in that the mixed-culture medium is The curved algae of bridge and shank algae mixed-culture medium, the curved algae of the bridge and shank algae mixed-culture medium by following parts by weight ingredient groups Into:12-18 parts of pretreated sanitary sewage, 0.04-0.09 parts of calcium chloride, 0.2-0.7 parts of potassium silicate, magnesium sulfate 0.03- 0.08 part, 0.03-0.08 parts of manganese sulfate, 0.005-0.009 parts of zinc chloride, 0.0003-0.0005 parts of copper chloride, frerrous chloride 0.02-0.07 parts, 0.0001-0.0003 parts of sodium molybdate, 0.0004-0.0007 parts of b diammonium edta sodium, cobalt chloride 0.004- 0.008 part, 0.006-0.008 parts of vanadic anhydride, 0.0004-0.0008 parts of vitamin B1, vitamin B12 0.0007- 0.0009 part, 0.0004-0.0007 parts of biotin, 0.002-0.008 parts of folic acid, 0.007-0.009 parts of inositol, compound medium-height grass 3-6 parts of liquid, 0.002-0.007 parts of heteroauxin, 1000 parts of distilled water;Wherein composite Chinese herbal liquid and preparation method thereof:By weight Portion rate takes 3-7 parts of glutinous rehmannia, 4-9 parts of radix bupleuri, 1-3 parts of matrimony vine, 8-12 parts of folium artemisiae argyi, 3-6 parts of purple perilla, 5-7 parts of the bark of eucommia, pure water 300 parts, heating, boils, and then slow boiling continues to boil 3 minutes, ceases fire, is cooled to room temperature, abandons the dregs of a decoction, taking liquid.
2. it is used for the mixed-culture medium of the purification method of city domestic sewage according to claim 1, it is characterised in that described Pretreated sanitary sewage pre-treatment step is as follows:First with the silk cover filtering sewage of 60 mesh, remove floating material, glass bits, Hair, silt, leaf, polybag larger solid content in equal volume, after sewage is introduced to 30 meters long, 20 meters wide, 0.8 meter deep cement In aeration tank, pond is in east-west, to receive more sunlights;The depth of water is maintained at 60 centimetres;By every cubic metre of sewage The amount for adding 60 grams of quick limes is uniformly splashed quick lime, every 30 minutes, then is added by every cubic metre of sewage 45 grams of the full pond of amount and is sprinkled Alum is spilt, is then fully aerated, stirred 60 minutes with air compressor machine, lime and alum are fully dissolved, are mixed and with having in sewage Machine thing fully chemically reacts, and when then natural sedimentation, fermentation, exposure 48 are small, skim the floating materials such as water surface foam, does not stir Bottom of pond, takes sewage at the middle and upper levels, is fitted into lighttight black plastic bucket, is sealed, spare.
3. the mixed-culture medium processing sanitary sewage of the purification method according to claim 1 for city domestic sewage Purification method, it is characterised in that
Step 1:The curved algae of bridge and shank algae inoculation method:The common plastics bucket that a volume is 40 liters is taken, adds claim 1 or 2 25 liters of the curved algae of the bridge and shank algae mixed-culture medium, then take in exponential phase of growth, clean, pollution-free, growth is vigorous The curved algae algae of bridge and shank algae algae be inoculated with respectively, inoculum density is bridge 4.5 ten thousand cells/mls of curved algae, shank algae 3.5 ten thousand A cells/ml, gently shakes up, and muffles bung hole with one block of plastic film, avoids polluting, stand-by;
Step 2:The curved algae of bridge and shank algae mixed culturing method:One 25 liters of vitreous distilled water bottle is taken, washes, scrubs Distilled water bottle after clean is sterilized 20 minutes using disinfectant again, and the disinfectant is Chinese herbal medicine composite disinfectant;The medium-height grass Medicine composite disinfectant constituent, by ratio of weight and the number of copies:Red 2-4 parts of dish of the back of the body, 6-9 parts of cordate houttuynia, 5-8 parts of radix bupleuri, Longtube Ground Ivy Herb 7- 12 parts, 10-15 parts of wrinkled giant hyssop, 7-12 parts of rhizoma atractylodis, 8-15 parts of the fruit of summer cypress, 5-7 parts of Chinese violet, ophiopogon 3-6 part, Radix Isatidis 0.5- 0.9 part, 10-14 parts of the tuber of stemona, 5-8 parts of Herba Andrographitis, 2-6 parts of dandelion, 1-3 parts of Phellinus;Then from the distilled water bottle bottle after disinfection Mouth puts small stone block into the 2/3 of distilled water bottle volume, and every piece of stone weight is 100-120 grams, then adds right from bottleneck It is required that the algae solution being inoculated with described in 3 is not untill coming stone block just;Bottle is sealed with 2 layers of clean newspaper and rubber band Mouthful, put this distilled water bottle into the culture of microdisk electrode room, culturing room's air-conditioning temperature control, fluorescent lamp together with algae solution therein and stone block Pipe provides illumination, carrys out regulating illumination intensity to open the quantity of fluorescent tube;Co-culture 8 days, intensity of illumination 6000lux be set, 25 DEG C of temperature, photoperiod are set to L16+D8, i.e., 16 it is small when illumination, 8 is dark when small, then again 16 it is small when illumination, 8 is dark when small, Move in circles;To the 8th day end, stop culture, it is 30 liters to put the tap of algae solution from Distilled water bucket bottom into a volume In plastic barrel, most of frustule is attached to gravelstone head surface at this time, and small part is in algae solution;
Step 3:Microalgae and water body separation method:5 grams are added by every liter of algae solution is pressed in the algae solution in described 30 liters of plastic barrel Amount add quick lime, be gently mixed, stand 8 it is small when, frustule and lime bits sink to bottom at this time, are drawn with siphon pipe The stillness of night, the clean water after as handling abandon bottom sediment, and purification is completed.
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