CN105753167A - Method and system for removing antibiotic resistance genes in aquaculture environment - Google Patents
Method and system for removing antibiotic resistance genes in aquaculture environment Download PDFInfo
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- CN105753167A CN105753167A CN201610316571.5A CN201610316571A CN105753167A CN 105753167 A CN105753167 A CN 105753167A CN 201610316571 A CN201610316571 A CN 201610316571A CN 105753167 A CN105753167 A CN 105753167A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/008—Mobile apparatus and plants, e.g. mounted on a vehicle
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a method and a system for removing antibiotic resistance genes in an aquaculture environment. The method comprises the following steps: (1) constructing an ecological floating bed in an aquaculture pond: the ecological floating bed is arranged in the aquaculture pond with aquatic organisms and aquatic plants are planted on the ecological floating bed; (2) constructing a vertical flow artificial wetland and a water circulation system of the vertical flow artificial wetland: the upper layer of the vertical flow artificial wetland is a soil layer, the lower layer thereof is a gravel layer, wetland plants are planted in the soil layer, water in the aquaculture pond successively flows through the soil layer and the gravel layer, and then reflows to the aquaculture pond to form the water circulation system after entering the vertical flow artificial wetland. The method and the system for removing the antibiotic resistance genes in the aquaculture environment can effectively lower COD (Chemical Oxygen Demand) and the content of ammonia nitrogen in the aquaculture environment, and further can effectively control spreading of the antibiotic resistance genes in the environment to effectively reduce harm of the antibiotic resistance genes in the aquaculture environment on human health and provide scientific and reliable technical support for human health and environmental protection.
Description
Technical field
The present invention relates to the water body purification technology in culture environment of aquatic products, particularly relate to a kind of remove the method and system of antibiotics resistance gene in culture environment of aquatic products.
Background technology
China is the big aquaculture big country of the first in the world, accounts for the 70% of world's cultivation total output.But in recent years due to aquaculture intensivization development, causing polluting aggravation, cause breeding environment quality constantly to worsen, causal organism kind increases to be accelerated with spread speed.The main method of current disease control is to use antibiotics, and antibiotic, except for disease preventing and treating, also has and promotes plant growing and save the effect of the aspects such as nutritional labeling.But owing to antibiotic use kind and quantity are lacked the constraint of corresponding rules and regulations by culture fishery, therefore, cause the antibiotic long-term abuse in culture fishery.Antibiotic a large amount of use not only can cause the antibiotic remains in aquatic animal body, and in use, antibiotic president's forward exchange in the antibiotic drained with feces and the feedstuff not eaten by biology combines in basic sediment, the pollutant of bed mud are in constantly accumulation and release process, thus causing the deterioration of aquaculture system integrated environment, remain in antibiotic in organism and culture environment of aquatic products, meeting inducing antibiotic resistance gene (antibioticresistancegenes, ARGs) produce, antibacterial is produced environmental selection pressure.
In recent years, antibiotics resistance gene is as a kind of novel environmental contaminants, increasingly paid attention to widely, be concentrated mainly on the pollution distribution of ARGs in the environment such as municipal sludge sewage, livestock and poultry farm, the mouth of the river, soil microbial community at present both at home and abroad about the research of ARGs.Research is pointed out, ARGs can persistently remain in the environment, can with Mobile Genetic Elements (mobilegeneticelements such as plasmid, transposon, phage, integrons, MGEs) combine, the boundary that hereditary material can only transmit between relationship is broken by Horizontal transfer effect, thus effectively propagating in bacterial community or between different bacterium colony, more microorganism is made to obtain this antibiotic resistance, and then propagate in the environment, spread, thus publilc health and food hygiene, drinking water safety etc. are constituted a threat to.But current research is also extremely limited for datas such as ARGs source in the environment, propagation and flooding mechanisms, and especially in culture environment of aquatic products, the pollution of ARGs not yet causes the enough attention of people.ARGs in aquaculture system is possible not only to aquaculture regional environment is caused direct pollution, can also by ecological circulation Transport And Transformation to evolutionary development in soil and Water Environment System, thus transferring to human body, Aquatic products consumption person and whole ecosystem cause huge latency environment pollute and health risk.
Therefore, finding the method that can effectively process the antibiotics resistance gene in culture environment of aquatic products is need the key issue of solution badly.
Summary of the invention
The shortcoming of prior art in view of the above, it is an object of the invention to provide and a kind of remove the method and system of antibiotics resistance gene in culture environment of aquatic products, for solving the problems such as the ARGs residual quantity height of aquaculture system in prior art, serious environment pollution.
For achieving the above object and other relevant purposes, first aspect present invention provides a kind of and removes the method for antibiotics resistance gene in culture environment of aquatic products, comprises the steps:
(1) build in aquaculture pond biological floating bed: cultivation have hydrobiological aquaculture pond is arranged biological floating bed, planting aquatic plants on biological floating bed.Water plant absorbs the organic substances such as N, the P in aquaculture pond water body and by growth metabolism effect purifying water body water quality mainly through aqueous bio, plays the effect removing antibiotics resistance gene.
(2) vertical current constructed wetland and water circulation system thereof are built: the upper strata of vertical current constructed wetland is soil horizon, lower floor is metalling, wetland plant is planted at soil horizon, after the water of aquaculture pond enters vertical current constructed wetland, flow through soil horizon, metalling successively, it is back to aquaculture pond again, forms water circulation system.
Further, in described step (1), it is 10-20% that the biological floating bed gross area accounts for the percentage ratio of aquaculture pond water surface area.
Further, in described step (1), the water plant of biological floating bed upper plantation is one or more combinations in water spinach, Herba Oenanthes Javanicae, Caulis Zizaniae caduciflorae, Canna generalis Bailey, the fire hose, Herba Eichhorniae, Rhizoma sagittariae sagittifoliae.
Further, in the aquaculture pond of described step (1), the aquatile of cultivation is fresh-water fishes.
Further, described fresh-water fishes are the one or more combination in Ctenopharyngodon idellus, Carassius auratus, Cyprinus carpio, Hypophthalmichthys molitrix.
Further, described step (1) biological floating bed for degradable polystyrene foam plate, this cystosepiment has degradable characteristic, environmental protection and energy saving, environmentally safe.
Further, in described step (2), the soil horizon thickness >=50cm of vertical current constructed wetland.
Further, in described step (2), the fractured-rock layer thickness of vertical current constructed wetland is 20～30cm.
Further, in described step (2), the ballast grain sizes of the metalling of vertical current constructed wetland is 5-20mm.
Further, in described step (2), the edaphic soil of vertical current constructed wetland is local soil or soil moved in improve the original, is specially organic abundant topsoil.
Further, in described step (2), the hydraulic detention time of vertical current constructed wetland is 10～15h.
Preferably, in described step (2), the hydraulic detention time of vertical current constructed wetland is 12h.
Further, in described step (2), on soil horizon, the wetland plant of plantation is one or more combinations in Herba Scirpi Tabernaemontani, phragmites communis, Rhizoma Acori Graminei, Mugil cephalus grass.
Further, in described step (2), the planting density of soil horizon wetland plant is: Herba Scirpi Tabernaemontani 50 strains/m2, 10 clumps/m of phragmites communis2, Rhizoma Acori Graminei 30 strains/m2, Mugil cephalus grass 15 strains/m2。
Second aspect present invention provides a kind of and removes the system of antibiotics resistance gene in culture environment of aquatic products, including aquaculture pond, vertical current constructed wetland, described aquaculture pond is provided with biological floating bed, biological floating bed upper kind is implanted with water plant, aquaculture pond is connected to vertical current constructed wetland by pipeline, described vertical current constructed wetland includes soil horizon and is positioned at the metalling bottom soil horizon, soil horizon kind is implanted with wetland plant, vertical current constructed wetland is connected to aquaculture pond again through pipeline, the water entering vertical current constructed wetland sequentially passes through soil horizon, metalling, it flow to aquaculture pond again through back of pipeline.
As mentioned above, the present invention removes the method and system of antibiotics resistance gene in culture environment of aquatic products, have the advantages that the process technique that invention introduces a set of advanced person, the degraded of antibiotics resistance gene in culture environment of aquatic products is removed and serves significant effect.Due to the method that comprehensively have employed microorganism, plant, chemistry, physics combine, it is possible not only to make the COD in aquaculture water, ammonia nitrogen to be removed, can also effectively remove antibiotics resistance gene in aquaculture system, moreover it is possible to by the recycling in addition of the water in aquaculture pond.Therefore, the method and system of the present invention can fast and effeciently remove antibiotics resistance gene in culture environment of aquatic products on the one hand, there is economic cost clear superiority such as low grade on the other hand, the water environment in purifying aquatic product cultivating pond, effectively reduce the content of this kind of polluter of antibiotics resistance gene in environment, effectively control the diffusion of antibiotics resistance gene, create the growing environment of health for aquaculture organisms, and then improve the safety of the food prepared by aquaculture organisms.
Accompanying drawing explanation
Fig. 1 is shown as the aquiculture waste water process chart of the present invention；
Fig. 2 layout schematic diagram that to be shown as on the aquaculture pond of the present invention biological floating bed；
Fig. 3 is shown as the biological floating bed plant fixing hole schematic diagram of the present invention；
Fig. 4 is shown as the vertical current constructed wetland generalized section of the present invention.
Detailed description of the invention
Below by way of specific instantiation, embodiments of the present invention being described, those skilled in the art the content disclosed by this specification can understand other advantages and effect of the present invention easily.The present invention can also be carried out by additionally different detailed description of the invention or apply, and the every details in this specification based on different viewpoints and application, can also carry out various modification or change under the spirit without departing from the present invention.
Embodiment 1
A kind of remove the method for antibiotics resistance gene in culture environment of aquatic products, comprise the steps:
(1) structure of biological floating bed botanical system
Raising together with Ctenopharyngodon idellus, Hypophthalmichthys molitrix, Carassius auratus, Cyprinus carpio in test pond, totally two groups, aquaculture pool area is 1 mu, depth of water 3m.The cultivation density of aquaculture pond is: Ctenopharyngodon idellus is 400 tails/mu, and stocking size is about 200g/ tail；Hypophthalmichthys molitrix is 200 tails/mu, and stocking size is about 200g/ tail；Carassius auratus and Cyprinus carpio are 100 tails/mu, and stocking size is about 150g/ tail.Control measures are consistent, and one group of fish pond is left intact, and build biological floating bed plant in one group of fish pond.The biological floating bed fish pond area that accounts for is 10%, biological floating bed test plant is water spinach, biological floating bed employing degradable polystyrene foam plate (300cm × 100cm × 10cm), with the field planting hole of spaced Φ=4 of 20～30cm～5cm in floating bed, 2～3 strain water spinach plant are implanted in every hole.Regularly water spinach can being plucked every 1 week, the water spinach of harvesting can be sold into market, produces economic benefit.Water spinach in biological floating bed botanical system is adsorbed mainly by organic substances such as N, the P in aquaculture water environment, plays the effect removing antibiotics resistance gene.The layout schematic diagram that to be illustrated in figure 2 in the present invention biological floating bed on aquaculture pond.
(2) structure of vertical current constructed wetland
The fundamental of vertical current constructed wetland is the microbial population in water penetration substrate (soil, rubble), wetland plant and soil matrix, and artificial swamp removes the mechanism of action of pollutant mainly by the microorganism contained in the substrate in wetland, wetland plant and substrate by physics, chemistry and biological agent synergistic purification water body.The material that the present embodiment is chosen is economic performance and the good rubble of absorption property, and soil is local soil or soil moved in improve the original, it is preferred to local soil, draw materials conveniently, the present embodiment selects the purple soil that the Chengdu Plain content of organic matter is abundant, and soil horizon thickness is 50cm, and fractured-rock layer thickness is 20cm.Soil horizon plantation plant is Rhizoma Acori Graminei, and planting density is 30 strains/m2, the vertical current constructed wetland gross area is 100m2, it is arranged to 10 ridges, every ridge arranges 3 water distribution pipes.In the present embodiment, owing to the level height of vertical current constructed wetland is higher than aquaculture pond, therefore, water pump is adopted to extract the water of aquaculture pond to regulating reservoir, regulating reservoir primarily serves the effect of the flow controlling water, simultaneously, it may have the effect of regulating water quality, entering vertical current constructed wetland then through water distribution pipe, the hydraulic detention time of vertical current constructed wetland is 12h.The water of vertical current constructed wetland is back to cultivation fish pond then through collecting conduit.Primarily serving the purpose of of vertical current constructed wetland removes the materials such as COD, ammonia nitrogen and antibiotics resistance gene further under the metabolism of wetland ecotourism and soil microorganism.
It is illustrated in figure 1 the aquiculture waste water process chart of the present invention, is illustrated in figure 3 the plant fixing hole schematic diagram that the present invention is biological floating bed, is illustrated in figure 4 vertical current constructed wetland generalized section of the present invention.
(3) project and method are measured
1. sampling: in 2 months of test, sample once every two weeks, choose cultivation have the fish of same number and kind and be not aquaculture pond water (sample number into spectrum: the 1-1 of any process, 1-2, 1-3, 1-4), the present embodiment is provided with biological floating bed aquaculture pond water (sample number into spectrum: 2-1, 2-2, 2-3, 2-4), the present embodiment vertical current constructed wetland water outlet (sample number into spectrum: 3-1, 3-2, 3-3, 3-4), each some position sample takes 3L water sample, 1L is for the test of basic physical and chemical index, 2L crosses two 0.22 μm of filter membranes, obtain the plankton in water body.
2. measure the basic physical and chemical index of water quality: include pH, ammonia nitrogen, COD content.
3. antibiotics resistance gene assay.
1) STb gene is extracted: according to FastDNATMThe operating procedure of SPINKitforSoil (MP, BIO) extracts the STb gene on filter membrane.
2) PCR reaction: PCR is polymerase chain reaction.Biliographic data information, determine selected ARGs primer: Tetracyclines resistant gene (tetA, tetM), sulfonamides resistant gene (sulI, sulII), quinolones resistant gene (qnrA, qnrB), I type integron (int1), transposon (Tn916/1545) and bacterial universal primers 16SrRNA universal primer (338F/518R).Determining antibiotics resistance gene PCR amplification system, the PCR amplification system solution used in this research is 25 μ L, including: 12.5 μ L2 × TaqPCRMastermix (sky root, Beijing), the 1 forward and reverse primer of μ L (10 μMs), 10ngDNA extract, ddH2O makes reaction system to 25 μ L.Pcr amplification condition is: enter 35 circulations after 94 DEG C of degeneration 5min, and each circulation includes 94 DEG C of reaction 30s, reacts 40s therewith under corresponding annealing temperature, afterwards annealing 90s at 72 DEG C again, after having circulated at 72 DEG C reaction 10min.Selected primer and corresponding annealing temperature are as shown in table 1.
The primer of table 1 antibiotics resistance gene, annealing temperature and fragment length
List of references 1:Li, D.etal.Determinationandfateofoxytetracyclineandrelatedco mpoundsinoxytetracyclineproductionwastewaterandthereceiv ingriver.EnvironmentalToxicologyandChemistry27,80-86 (2008).
List of references 2:Aminov, R., Garrigues-Jeanjean, N.&Mackie, R.Molecularecologyoftetracyclineresistance:developmentan dvalidationofprimersfordetectionoftetracyclineresistance genesencodingribosomalprotectionproteins.Appliedandenvir onmentalmicrobiology67,22-32 (2001).
List of references 3:Pei, R., Kim, S.-C., Carlson, K.H.&Pruden, A.Effectofriverlandscapeonthesedimentconcentrationsofant ibioticsandcorrespondingantibioticresistancegenes (ARG) .WaterResearch40,2427-2435 (2006).
List of references 4:Wu, J.-J., Ko, W.-C., Tsai, S.-H.&Yan, J.-J.Prevalenceofplasmid-mediatedquinoloneresistancedete rminantsQnrA, QnrB, andQnrSamongclinicalisolatesofEnterobactercloacaeinaTaiw anesehospital.Antimicrobialagentsandchemotherapy51,1223-1227 (2007).
List of references 5:Goldstein, C.etal.Incidenceofclass1and2integrasesinclinicalandcomme nsalbacteriafromlivestock, companionanimals, andexotics.Antimicrobialagentsandchemotherapy45,723-726 (2001).
List of references 6:Roberts, A.P.etal.TransferofTn916-likeelementsinmicrocosmdentalpl aques.Antimicrobialagentsandchemotherapy45,2943-2946 (2001).
List of references 7:Koike, S.etal.Monitoringandsourcetrackingoftetracyclineresistan cegenesinlagoonsandgroundwateradjacenttoswineproductionf acilitiesovera3-yearperiod.Appliedandenvironmentalmicrob iology73,4813-4823 (2007).
3) ARGs real-time quantitative PCR detection (qPCR)
PCR primer purification reclaims: use purification kit to be purified recovery PCR primer.
Prepared by plasmid: the segment genome after reclaiming is connected to pGEM-TEasy carrier (Promega, USA), then in transformed competence colibacillus cell E.coliDH5 α, is applied in the LB culture medium of Amp-Xgal/IPTG/X-Gal, cultivates 16h～24h at 37 DEG C.2-3 white macula clone of random choose carries out regular-PCR.By PCR result comparison, the positive colony bacterium solution that picking target fragment and known products length meet.
With reference to the explanation extracting test kit, utilize the plasmid of plasmid extraction kit (Tiangen, Beijing) the correct clone of abstraction sequence, measure its DNA concentration, and it is become real-time fluorescence quantitative PCR bioassay standard product with 10 times for interval serial dilution.Concentration according to plasmid DNA, and in conjunction with the molecular weight of plasmid DNA, calculate copy number (the μ L of plasmid DNA-1)。
Plasmid DNA molecule amount=(length of the purpose fragment length+cloning vehicle of insertion)/660
The making of standard curve and real-time fluorescence quantitative PCR: adopt real time fluorescence quantifying PCR method to measure ARGs content in sample, sample amounts: Real-TimePCR reaction system is 25 μ L, including 2ng template DNA, 10 μ L2.5 × LSYBRRealMasterMix (TianGen, Beijing), 1.5 μ Lof20 × SYBR solution (TianGen, Beijing), each primer final concentration 4pmol, reaction carries out in Axygen company 200 μ L dome PCR pipe.QPCR response procedures is: after 94 DEG C of 2min of denaturation, 94 DEG C of degeneration 15s, and with reference to annealing 20s under each primer annealing temperature in table 1,68 DEG C extend 30s, 40 circulations.In each circulation, phosphor collection carries out after extension.Sample is carried out together with standard substance Real-TimePCR detection, includes each sample of negative control and set 3 repetitions.
Calculate the gene copy number in sample according to standard curve, utilize following equation, calculate and obtain each gene copy number in every gram of sample:
(4) content of antibiotics resistance gene in each process section water body, the content of integron and relative amount testing result such as table 2 below to 16SrRNA, learnt by table 2 below, in vertical current constructed wetland water outlet place, it is not detected by quinolone antibiotic resistant gene qnrA and qnrB.
The content of antibiotics resistance gene in each process section water body of table 2
Illustrate: copy number, unit copies/mL；Relative amount, log (16SrRNA gene dosage/all kinds of resistant gene content)；ND represents and does not detect
Calculating corresponding clearance according to the content results of antibiotics resistance gene in each process section water body, when the 30th day, the clearance of tetracycline antibiotics resistant gene tetA reached 99.999%, and the clearance of tetB has reached 99.995%；Sulfonamides resistant gene sulI clearance when the 60th day has reached 99.995%, and sulII reaches 99.997%；Vertical current constructed wetland water outlet place qnrA and qnrB has reached not detect level；Transposon intI clearance when the 60th day has reached 99.999%.
Measured COD, ammonia nitrogen are added up simultaneously, when the 60th day ammonia nitrogen in water body, COD clearance respectively reached 85.7% and 90.5%.
In sum, antibiotics resistance gene in culture environment of aquatic products is removed by the present invention mainly through biological floating bed and vertical current constructed wetland, on the one hand, antibiotics resistance gene in water body is carried out original position removal by biological floating bed in aquaculture pond, on the other hand, after water in aquaculture pond flow to vertical current constructed wetland, antibiotics resistance gene in water body is carried out dystopy removal by artificial swamp, the water of artificial swamp is back to aquaculture pond after treatment, realize recycling of water, the method can effectively reduce COD and ammonia nitrogen in culture environment of aquatic products, can effectively control again antibiotics resistance gene propagation in the environment, effectively prevent the antibiotics resistance gene harm to human health in culture environment of aquatic products, science and reliable technical support is provided for human health and environmental conservation.
Above-described embodiment is illustrative principles of the invention and effect thereof only, not for the restriction present invention.Above-described embodiment all under the spirit and category of the present invention, can be modified or change by any those skilled in the art.Therefore, art has usually intellectual such as modifying without departing from all equivalences completed under disclosed spirit and technological thought or change, must be contained by the claim of the present invention.
Claims (10)
1. remove the method for antibiotics resistance gene in culture environment of aquatic products for one kind, it is characterised in that comprise the steps:
(1) build in aquaculture pond biological floating bed: cultivation have hydrobiological aquaculture pond is arranged biological floating bed, planting aquatic plants on biological floating bed；
(2) vertical current constructed wetland and water circulation system thereof are built: the upper strata of vertical current constructed wetland is soil horizon, lower floor is metalling, wetland plant is planted at soil horizon, after the water of aquaculture pond enters vertical current constructed wetland, flow through soil horizon, metalling successively, it is back to aquaculture pond again, forms water circulation system.
2. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in described step (1), it is 10-20% that the biological floating bed gross area accounts for the percentage ratio of aquaculture pond water surface area.
3. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterized in that: in described step (1), the water plant of biological floating bed upper plantation is one or more combinations in water spinach, Herba Oenanthes Javanicae, Caulis Zizaniae caduciflorae, Canna generalis Bailey, the fire hose, Herba Eichhorniae, Rhizoma sagittariae sagittifoliae.
4. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in the aquaculture pond of described step (1), the aquatile of cultivation is fresh-water fishes.
5. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in described step (2), the soil horizon thickness >=50cm of vertical current constructed wetland.
6. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in described step (2), the fractured-rock layer thickness of vertical current constructed wetland is 20～30cm.
7. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in described step (2), the edaphic soil of vertical current constructed wetland is local soil or soil moved in improve the original.
8. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterised in that: in described step (2), the hydraulic detention time of vertical current constructed wetland is 10～15h.
9. the method for antibiotics resistance gene in removal culture environment of aquatic products according to claim 1, it is characterized in that: in described step (2), on soil horizon, the wetland plant of plantation is one or more combinations in Herba Scirpi Tabernaemontani, phragmites communis, Rhizoma Acori Graminei, Mugil cephalus grass.
10. remove the system of antibiotics resistance gene in culture environment of aquatic products for one kind, it is characterized in that, including aquaculture pond, vertical current constructed wetland, described aquaculture pond is provided with biological floating bed, biological floating bed upper kind is implanted with water plant, aquaculture pond is connected to vertical current constructed wetland by pipeline, described vertical current constructed wetland includes soil horizon and is positioned at the metalling bottom soil horizon, soil horizon kind is implanted with wetland plant, vertical current constructed wetland is connected to aquaculture pond again through pipeline, the water entering vertical current constructed wetland sequentially passes through soil horizon, metalling, it flow to aquaculture pond again through back of pipeline.
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| CN106698652A (en) * | 2016-12-13 | 2017-05-24 | 同济大学 | Method for removing antibiotics resistance gene in sewage |
| CN109734247A (en) * | 2019-01-30 | 2019-05-10 | 中国环境科学研究院 | A kind of ecological waste water processing and utilizing system |
| CN112777822A (en) * | 2021-01-12 | 2021-05-11 | 浙江科技学院 | Method for intercepting antibiotic resistance genes in aquaculture tail water by using straw charcoal |
| CN113875631A (en) * | 2021-08-23 | 2022-01-04 | 河海大学 | Chicken manure ecological treatment and recycling system and operation method |
| CN114477459A (en) * | 2022-01-17 | 2022-05-13 | 哈尔滨工业大学 | Method for reducing antibiotic resistance genes in aquaculture wastewater |
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| CN113875631B (en) * | 2021-08-23 | 2022-09-30 | 河海大学 | Chicken manure ecological treatment and recycling system and operation method |
| CN114477459A (en) * | 2022-01-17 | 2022-05-13 | 哈尔滨工业大学 | Method for reducing antibiotic resistance genes in aquaculture wastewater |
| CN114477459B (en) * | 2022-01-17 | 2023-02-07 | 哈尔滨工业大学 | Method for reducing antibiotic resistance genes in aquaculture wastewater |
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