CN105750212B - A kind of screening technique of the kind of super enrichment huge sum of money platymiscium - Google Patents
A kind of screening technique of the kind of super enrichment huge sum of money platymiscium Download PDFInfo
- Publication number
- CN105750212B CN105750212B CN201610250906.8A CN201610250906A CN105750212B CN 105750212 B CN105750212 B CN 105750212B CN 201610250906 A CN201610250906 A CN 201610250906A CN 105750212 B CN105750212 B CN 105750212B
- Authority
- CN
- China
- Prior art keywords
- plant
- huge sum
- soil
- seed
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
Abstract
The invention provides a kind of screening technique of the kind of super enrichment huge sum of money platymiscium, it is related to agricultural technology field and soil remediation technology field.This method includes setting screening criteria concentration, chooses the kind of primary dcreening operation plant, screens the several steps of kind of super enrichment huge sum of money platymiscium.This method operation element amount is small, and demand area is small, and cost is low, and selection cycle is short, and as a result stability is good, and practicality is high, suitable for large-scale promotion.
Description
Technical field
The present invention relates to agricultural technology field and soil remediation technology field, in particular to a kind of super enrichment huge sum of money
The screening technique of the kind of platymiscium.
Background technology
Soil is one of important resource of human society, is developing agricultural, industry and carries out urban modernization construction
Material base.But lived due to digging up mine with smelting, industry and the mankind such as traffic emission, discarded object stacking, agricultural chemicals and fertilizer application
Dynamic strong influence, soil pollution getting worse.The especially exploitation and smelting of mineral resources, grand strategy money is provided for country
While source, the serious consequences such as environmental pollution, geological disaster, ecological disruption are also result in.
And in numerous remediation contaminated soil methods, compared with traditional chemically and physically Treatment process, phytoremediation
(phytoremediation) by its is economical, environment-friendly, can keep the features such as soil productivity, non-secondary pollution, by
The extensive concern of academia.Phytoremediation technology using plant ultraproduct it is tired or accumulate the Phytoextraction reparation of sexual function,
Waved using the Phytostabilization of root system of plant control contamination and recovery ecological functions, using the plant of Plant Transformation function
Hair is repaired.At present, it is believed that it is not very high agricultural land soil that Phytoextraction reparation, which is applied to heavy metal pollution degree, pass through
The super enrichment huge sum of money platymiscium of several seasons or several years is planted, then the heavy metal accumulation in soil to aboveground vegetation part uses normal
Further handled after rule agricultural methods harvest, heavy metal-polluted soil concentration is dropped to below standard of soil environment quality, make grain
Food crop can keep the safety in production.And plant stability technology is then more suitable for the ecology in high heavy metal pollution place caused by industry and mining
Recover, the diffusion mobility of heavy metal element caused by reduce rainwash erosion, wind erosion etc..
In the prior art, the kind of super enrichment huge sum of money platymiscium is screened, is first had in highly polluted regional observation energy
Enough growing plants, collection then is sampled to the plant for there are enrichment potentiality, all using the foster side of Liquid Culture or earth culture
Formula is cultivated, and then checks the content of heavy metal in soil, plant shoot and root, determines whether that super enrichment heavy metal is planted
The kind of thing.Such a method workload is big, and demand area is big, and cost is high, and cycle length, as a result stability is also poor.
The content of the invention
It is an object of the invention to provide a kind of screening technique of the kind of super enrichment huge sum of money platymiscium, this screening technique tool
There is the advantages of workload is small, and demand area is small, and cost is low, and selection cycle is short.
The present invention is solved its technical problem and realized using following technical scheme：
A kind of screening technique of the kind of super enrichment huge sum of money platymiscium, comprises the following steps：
Set screening criteria concentration：The more seeds of plant by pretreatment are respectively put into different multiple heavy of concentration
In metallic test solution, germination culture is carried out, chooses the Heavy metal solution where the seed for the plant that seedling-erecting ratio is 50%
Concentration be screening criteria concentration, seedling-erecting ratio=(B/C) X 100%, B is upright seedling number, and C is germination number；
Choose the kind of primary dcreening operation plant：From multiple kinds of plant, the seed of each kind by pretreatment is put
Enter in the heavy metal screening solution with screening criteria concentration, carry out germination culture, calculate the seedling-erecting ratio of the seed of each kind,
Select the kind that kind corresponding to preceding 10~20% high seed of seedling-erecting ratio is primary dcreening operation plant；And
Screen the kind of super enrichment huge sum of money platymiscium：The seed of the kind of each primary dcreening operation plant by pretreatment is distinguished
It is seeded in more parts of different soil of heavy metal concentration, and carries out field management, until it is grown to plant, determines every part
The content of beary metal of root and the weight of plant shoot of plant corresponding to the content of beary metal of soil and every part of soil
Tenor, when plant survival, and the content of beary metal of its plant shoot is higher than the content of beary metal of root, and
The substance withdrawl syndrome of its plant shoot is more than the substance withdrawl syndrome of root, and the huge sum of money before the soil remediation where it
When belonging to the content of beary metal that content is more than after administering, the product that the kind belonging to plant is super enrichment huge sum of money platymiscium are determined
Kind.
Relative to prior art, the present invention includes following beneficial effect：The present invention is by setting multiple test concentrations, then
The selection of science is carried out according to half lethal concentration, the base concentration that plant can adapt to is determined, obtains normal concentration；Then,
Using step-sizing method, preliminary screening is carried out with the sensitive sprouting stage of plant heavy metal；It is further by potted plant mode again
Screening, determines the adsorption capacity of plant heavy metal, the product of super enrichment huge sum of money platymiscium is determined by detecting content of beary metal
Kind.Wherein, seedling-erecting ratio concept is introduced, preliminary screening is carried out using the very sensitive sprouting stage of plant heavy metal, is eliminated pair
The material of heavy metal sensitive, significantly reduces workload, improves operating efficiency, by calculating seedling-erecting ratio come rapid, accurate
Carry out screen.Because when the seed of plant germinates under the stress of heavy metal environment, in fact it could happen that：(1) seed is not sprouted；
(2) after seed is sprouted, because the amount of the heavy metal of absorption is more in subsequent several days, the growth of the root of seed is suppressed, root
Short, not long root or root blackening, it can not uprightly be lieed down；(3) seed can be sprouted, and root growth is normal, cauline leaf
Can normal growth, such a is just the stronger kind of super enrichment ability.
Being additionally, since the plant germination phase is generally several days, it is possible to is repeatedly screened in a short time, plant sprouting stage
Biological volume it is also smaller, area occupied is small, interior can just be screened, also not by natural causes such as rainwater in experimentation
Influence, be easy to manual adjustment.This method is simple to operate, and cost input is less, and it makes use of multiple plant varieties, multiple heavy metals
Concentration is tested, and scope of experiment is big, and data are reliable, and as a result stability is preferable, and selection cycle is also short.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
The screening technique of the kind of the super enrichment huge sum of money platymiscium of the embodiment of the present invention is specifically described below.
The screening technique of the kind of super enrichment huge sum of money platymiscium, comprises the following steps：
Set screening criteria concentration：The more seeds of plant by pretreatment are respectively put into different multiple heavy of concentration
In metallic test solution, germination culture is carried out, chooses the Heavy metal solution where the seed for the plant that seedling-erecting ratio is 50%
Concentration be screening criteria concentration, seedling-erecting ratio=(B/C) X 100%, B is upright seedling number, and C is germination number；
Choose the kind of primary dcreening operation plant：From multiple kinds of plant, the seed of each kind by pretreatment is put
Enter in the heavy metal screening solution with screening criteria concentration, carry out germination culture, calculate the seedling-erecting ratio of the seed of each kind,
Select the kind that kind corresponding to preceding 10~20% high seed of seedling-erecting ratio is primary dcreening operation plant；And
Screen the kind of super enrichment huge sum of money platymiscium：The seed of the kind of each primary dcreening operation plant by pretreatment is distinguished
It is seeded in more parts of different soil of heavy metal concentration, and carries out field management, until it is grown to plant, determines every part
The content of beary metal of root and the weight of plant shoot of plant corresponding to the content of beary metal of soil and every part of soil
Tenor, when plant survival, and the content of beary metal of its plant shoot is higher than the content of beary metal of root, and
The substance withdrawl syndrome of its plant shoot is more than the substance withdrawl syndrome of root, and the huge sum of money before the soil remediation where it
When belonging to the content of beary metal that content is more than after administering, the product that the kind belonging to plant is super enrichment huge sum of money platymiscium are determined
Kind.
Wherein, upright seedling be cotyledon green can adhere to without lodging sign, root without yellow, stem and can absorb water, can be normal
The seedling of growth.
In the present invention, heavy metal solution is at least one of the hydrate soln of caddy 2/5, nitric acid cadmium solution.It is preferred that
The hydrate soln of caddy 2/5.Certainly, such a setting is not intended as limiting, in fact, heavy metal solution should be according to actual weight
Metallic pollution is set, and chooses experimental solutions, simulating pollution environment.Administered as ciliate desert-grass can be selected in arsenic pollution, lead contamination
Roundleaf penny cress can be selected to be administered.
If heavy metal solution selects cadmium-containing solution, plant is preferably rape.Because nursery it is experimentally confirmed that rape for cadmium
The reparation of pollution is optimal plant, and it has some following advantage：
(1) rape has superpower adsorption capacity to cadmium, is to accumulate one of horizontal highest plant of heavy metal, rape in the world
2000ppm is reached to the accumulation capability of cadmium, 1000ppm is reached to the accumulation capability of zinc, in general, the purification cycle of rape is 3~5
Year；
(2) Oilseed rape biomass is big, is easy to gather in, and its climatic adaptation ability is also relatively strong, and rape belongs to winter unique large oil
To expect crop, and rice soil out of question, the income of peasant will not be reduced, south rice field major part winter leaves unused, and light warm water is sufficient,
It is adapted to rapeseed cultivation；
(3) if the vegetable seed planted out is free of cadmium, the oil squeezed out can be used for eating；If the vegetable seed planted out contains cadmium,
The oil then squeezed out can be used for power plant, avoids throwing aside and causes the secondary pollution of cadmium；
(4) china rape refers generally to cabbage type rape, belongs to same category with Indian mustard, two species genes have height
Homology is spent, is easy to filter out and select from cabbage type rape the kind of super enrichment huge sum of money platymiscium.
Certainly, the selection of rape is not intended as limiting, and the selection of plant should be depending on actual heavy metal pollution.If
Cadmium pollution, then except rape, plant is also an option that as Thlaspi caerulescens, Sedum alfredii Hance, pteridophyte maidenhair spleenwort, wild amaranth
Deng, simply Sedum alfredii Hance, pteridophyte maidenhair spleenwort, the biomass of wild amaranth are too small, and plant is shorter, harvest be difficult to machinery
Change, the absolute magnitude of cadmium is very limited.It is weaker in the adaptability of China and the biomass of Indian mustard is less than normal, it is used for
Limited potential is administered in cadmium pollution.
For the ease of describing, in embodiments of the invention, heavy metal solution is disposed as cadmium solution, and plant is from oil
Dish.
When setting screening criteria concentration, Comprehensive Traits can be selected excellent or promote mainly the 1~2 of kind variety seeds,
Then concentration gradient reasonable in design, as 10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg,
Seven cadmium solutions of 120mg/kg (Heavy metal solution) processing and a control treatment, each processing at least two repeat.When
So, it can also be provided that other concentration gradients, such as 30mg/kg, 50mg/kg, 70mg/kg.
When setting screening criteria concentration, vegetable seeds is respectively put into the different multiple Heavy metal solution of concentration
In, the seed in each culture dish carries out the processing of the Heavy metal solution of a concentration, if any seven cadmium solution (heavy metals
Test solution) processing and a control treatment, then need to use eight culture dishes, corresponding one of each culture dish is handled；If each place
Two repetitions are managed, then needs again plus eight culture dishes, each processing corresponds to two culture dishes.Load certain amount in each culture dish
Seed, such as 50,43,66.In the pretreatment of setting screening criteria concentration step, including will be each
Seed in culture dish carries out sterilizing.4~6g/kg medicining liquid dipping 8~12min of seed can be utilized, in this way, energy
Enough prevent later stage seed from going mouldy, while the operation also has the function that to urge kind；Then, using water cleaning seed is handled, wash
Fall the thimerosal of the surface of the seed attachment, avoid influenceing later experiments.Wherein, thimerosal is sodium hypochlorite, liquor potassic permanganate, liter
At least one of mercury solution, and water is handled as at least one of deionized water after pure water, ultra-pure water, sterilizing, from this
A little water are handled, and can effectively avoid introducing bacterium.
In addition, when to seed sterilizing, can also be before using thimerosal, first with 700 in order to sterilize thoroughly
~800g/kg alcohol-pickled 4~6min of seed, then pours out alcohol, adds thimerosal processing, after thimerosal processing,
Thimerosal is poured out, then continuously cleans seed 2~4 times with pure water, then cleaned one time with ultra-pure water or sterilized water for injection.
Moved on in the pretreatment of setting screening criteria concentration step and then by seed in culture dish, while repetition is set
And control, then toward the cadmium solution (Heavy metal solution) that 3~5ml of addition is prepared in culture dish, carry out mark.In seed
Shifting put during, 2~3 layers of filter paper of place mat preferably under seed, in this way, after cadmium solution is added dropwise, seed can more preferably also more
It is uniform to absorb cadmium solution.And when preparing cadmium solution, prepared preferably by the hydrate of caddy 2/5 and processing water.
Then, all culture dishes are placed in 22~25 DEG C of temperature, periodicity of illumination is bright 15~17h of phase, dark 7~9h of phase
Germination culture is carried out in environment.Until the 6th~8 day, count seedling-erecting ratio.Need to calculate upright seedling number in seedling-erecting ratio, i.e., upright seedling
Quantity, upright seedling refer to cotyledon green without yellow, and stem is without lodging sign, and root can be adsorbed on filter paper and can absorbed water, Neng Gouzheng
The seedling being frequently grown.Certainly, if not being lined with filter paper in culture dish, it can observe whether its root can adhere to and can absorb water.This
Outside, when counting seedling-erecting ratio, germination percentage can also be counted simultaneously, so, it is possible to ensure that experimental data is more accurate, reliable.One
As for, the normal concentration of homozygous rape seed is 60~80mg/kg, and the normal concentration of cross-bred rape seed be 80~
120mg/kg。
In the step of choosing the kind of primary dcreening operation plant, (exist in the pretreatment that seed is carried out to above-mentioned sterilizing
The pretreatment of the sterilizing carried out in setting screening criteria concentration) before, pretreatment can also include：By seed 20~35
20~30h is dried at DEG C.It so, it is possible the germination percentage of raising seed.After the pretreatment of sterilizing is carried out, seed is put into
It is lined with the culture dish of 2~3 layers of filter paper, sets up repetition and control, is added dropwise into culture dish (except the culture dish of control experiment)
The cadmium solution (heavy metal screening solution) of normal concentration, then carries out germination culture.Certainly, the condition of the germination culture also with it is upper
The condition for stating germination culture (the germination culture carried out in screening criteria concentration is set) is identical.Again when germination, meter
Calculate germination percentage and seedling-erecting ratio.
Germination percentage=(chitting piece number/seed sum) X 100%,
Seedling-erecting ratio=(upright seedling number/chitting piece number) X 100%.
During selection, regardless of whether with reference to germination percentage, it is corresponding that preceding 10~20% high seed of seedling-erecting ratio can be chosen
Plant variety be primary dcreening operation plant kind because seedling-erecting ratio is high, then in general, germination percentage is also high.
In the kind of screening super enrichment huge sum of money platymiscium, the heavy metal concentration of more parts of soil is to be handled through concentration gradient
Concentration, process range are.
Concentration gradient can be established as：10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg,
And for example 75mg/kg, 80mg/kg, 85mg/kg, 90mg/kg, 95mg/kg, 100mg/kg, 105mg/kg.Utilize the water of caddy 2/5
Polymer solution and/or nitric acid cadmium solution are handled, and are handled preferably by the hydrate soln of caddy 2/5.Chlorination can be weighed
The hydrate of cadmium 2/5, it is dissolved in above-mentioned processing water, according to concentration needs, solution is made, sprayer can be utilized, by various concentrations
Spray solution in soil, more parts of different soil of heavy metal concentration are made.It is noted that in order to avoid later experiments
Accuracy so that each position content of beary metal of the plant grown up to is uniform, so needing to mix heavy metal solution and soil
It is even.And in order to realize the purpose, can before soil mixes with solution, first make soil through air-drying, sort, picking, be levigate,
Sieving, which is dried, crushed, sieve etc., to be handled, and ensures the gas permeability and mellowness of soil, so that it is preferably well mixed with solution.
Before the seed sowing of the kind of each primary dcreening operation plant, it can also be pre-processed, i.e., be dried seed
Processing, to improve the germination percentage of seed.Then sow, then apply fertilizer to every part of soil, the mass ratio of fertilizer and soil is 5:
7000~8000.Fertilizer can provide nutrient necessary to one or more kinds of plants, and can improve soil property, improve
Soil fertility level.In fertilizer, nitrogen content is 12~18%, and phosphorus content is 12~18%, and potassium content is 12~18%.Applying fertilizer
Before, fertilizer can be dissolved in a small amount of water, dissolve fertilizer, form fertilizer solution, spray fertilizer solution to be applied fertilizer, in this way,
Fertilising is more uniform.Then normal field management is carried out, until seed growth is plant, then determines cadmium content.Measure
Cadmium content can be further：After being set seeds respectively before plant bolting with plant blossom, the content of beary metal of every part of soil is determined
And the content of beary metal of the content of beary metal of the root of plant and plant shoot corresponding to every part of soil.It is in this way, logical
Detection is crossed, is capable of determining that the rape in which period is higher to the bioaccumulation efficiency of cadmium, because the main long-term cropping of certain areas
Be not rape, if bioaccumulation efficiency is relatively higher during rape bolting, according to plant growth situation, illustrate rape seedling stage just
Through the purpose that can reach rehabilitating soil, then can just pull out rape before rape bolting, continue to plant under this area
Season crop, in this way, the normal plantation of these regional crops would not be influenceed.In addition, after plant blossom is set seeds, can also be simultaneously
The content of beary metal for the seed that plant corresponding to determining every part of soil is born.By the test, it is determined that in the vegetable seed born
Cadmium content, so as to determine the purposes of vegetable seed, if cadmium content is no more than food sanitation standard (cadmium content≤0.2mg/kg) in vegetable seed, press
The oil squeezed out can be used for eating；If cadmium content exceedes food sanitation standard in vegetable seed, the oil squeezed out can be used for bioelectrogenesis
Factory.Finally according to hyperaccumulative plant or the selection standard of super enrichment huge sum of money platymiscium, the kind of super enrichment huge sum of money platymiscium is determined.
Wherein, when determining cadmium content, can be determined using the methods of atomic absorption spectrography (AAS).It is and rich in screening excess of export
After the kind for collecting huge sum of money platymiscium, it can be demonstrated in cadmium pollution, pushed away than more serious area according to the growth characteristic of kind
Wide and application, verifies its concentration effect to cadmium.
More than, rape can select the cabbage type rape planted extensively to be tested.
The feature and performance of the present invention are described in further detail with reference to embodiments：
Embodiment one
Embodiments of the invention one provide a kind of super enrichment rape screening technique, comprise the following steps,
A. 400 seeds are selected, seed 8min is soaked using 4g/kg sodium hypochlorite, then pours out sodium hypochlorite, continuously
With pure water by wash seeds 2 times；
B. the hydrate of caddy 2/5 and pure water are utilized, sets up 10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/
The cadmium test solution of 7 concentration gradients of kg, 100mg/kg, 120mg/kg；
C. 400 rape seeds are divided into 8 parts, be individually positioned in 8 culture dishes, number consecutively is 1~No. 8, often
Individual 50 rape seeds of culture dish, wherein 1~No. 7 culture dish be added dropwise successively 3ml concentration be 10~120mg/kg cadmium test it is molten
Liquid, retain the clear water that equivalent is added dropwise in No. 8 culture dishes, as a control group, set up 2 repetitions；
D. step c all culture dishes are placed in 22 DEG C, periodicity of illumination is bright phase 15h, in dark phase 9h environment, is sent out
Bud culture, the 6th day statistics seedling-erecting ratio, the concentration for choosing the cadmium test solution that No. 4 culture dishes are added dropwise is screening criteria concentration；
E. from blue or green oil 14, blue or green oil 331, Zhejiang oil 601, in it is double No. 4, in it is double No. 8, in it is double No. 9, in it is double No. 10, in it is double
No. 11, rich oil 6, Zhejiang oil 50, Zhejiang oil 601, Zhejiang oil 50, oil grind No. 10, middle oily 6766, holy light 402, river oil 36, river
Oil 37, China's oil are miscellaneous 12, China's oil is miscellaneous No. 6, oily 100 rape varieties such as No. 8 of DS, each 50 seeds of variety adoption, by each product
The rape seed of kind is pre-processed using step a method；Then the seed of pretreated 100 rape varieties is put
Entering in 100 culture dishes, the screening criteria concentration that 3ml steps d confirms is added dropwise in each culture dish, sets up control and 2 repetitions, then
All culture dishes are placed in 22 DEG C, periodicity of illumination is bright phase 15h, in dark phase 9h environment, carries out germination culture；Count within 6th day
The seedling-erecting ratio of the seed of each rape variety, select preceding 10% high seed of seedling-erecting ratio corresponding to rape variety be primary dcreening operation rape
Kind, as oil grind 817, in double 10 kinds such as No. 11；
F. utilize the hydrate of caddy 2/5 and pure water, prepare cadmium solution, then take 10 groups of Culture basins, be designated as the 1st group~
10th group, every group of 7 Culture basins, label 1~7, every group is taken 6 parts of soil, sprays cadmium solution respectively into per a soil,
It is well mixed, the cadmium solution concentration for making 6 parts of soil be respectively 10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg,
100mg/kg, be then uniformly mixed with the soil of cadmium solution by 6 parts and be fitted into 6 Culture basins, according to 10mg/kg, 20mg/kg,
40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg concentration order are fitted into 1~No. 6 Culture basin, then take 1 part of soil, past
Isometric aqueous solution is wherein sprayed, is then charged into No. 7 Culture basins, as a control group；
G., the seed that oil is ground to 817 10 kinds of grade is all divided into 7 parts, every part of 50 seeds, in conventional seed planting season, divides
It is not seeded in the 1st group~the 10th group of Culture basin, then carries out field management, until seed is all grown to rapeseed plants, then
Determine the cadmium content of the soil in each Culture basin, rapeseed plants corresponding to every part of soil root cadmium content and Plant aboveground
The cadmium content in portion, the selection standard of super enrichment rape is contrasted according to the cadmium content before and after soil remediation, double No. 11 kinds in learning
The cadmium content in soil is reduced, and meets the selection standard of super enrichment rape, is defined as super enrichment rape variety.
Embodiment two
Embodiments of the invention two provide a kind of super enrichment heavy metal foliage filter screening method, comprise the following steps,
A. 315 seeds are selected, seed 12min is soaked using 6g/kg liquor potassic permanganate, then pours out potassium permanganate
Solution, continuously with pure water by wash seeds 4 times；
B. cadmium nitrate and ultra-pure water are utilized, set up 30mg/kg, 50mg/kg, 70mg/kg, 90mg/kg, 110mg/kg,
The cadmium test solution of 6 concentration gradients of 130mg/kg；
C. 315 rape seeds are divided into 7 parts, be individually positioned in 7 culture dishes, number consecutively is 1~No. 7, often
Individual 45 rape seeds of culture dish, wherein 1~No. 6 culture dish be added dropwise successively 5ml concentration be 30~130mg/kg cadmium test it is molten
Liquid, retain the clear water that equivalent is added dropwise in No. 7 culture dishes, as a control group, set up 3 repetitions；
D. step c all culture dishes are placed in 25 DEG C, periodicity of illumination is bright phase 17h, in dark phase 7h environment, is sent out
Bud culture, the 8th day statistics seedling-erecting ratio, the concentration for choosing the cadmium test solution that No. 5 culture dishes are added dropwise is screening criteria concentration；
E. from wide source 58, contain Hunan oil No. 9, oil grind No. 10, moral oil No. 158, kernel oil 202, China oil it is miscellaneous No. 12, often it is miscellaneous
Oil No. 2, China oil it is miscellaneous No. 62, magnificent Hunan oil No. 10, holy light 77, Keeping high quality, China oil it is miscellaneous No. 15, Zhejiang oil No. 28, oil grind No. 817, China
Hunan oil 12, moral oil 5, spring cloud 763, Deyou 8, rich oil are miscellaneous No. 108, oily 150 rape varieties such as No. 737 of Feng, each
45 seeds of variety adoption, the rape seed of each kind is pre-processed using step a method；Then will pretreatment
The seed of 150 rape varieties afterwards is put into 150 culture dishes, and the screening criteria that 5ml steps d confirms is added dropwise in each culture dish
Concentration, control and 3 repetitions are set up, then all culture dishes are placed in 25 DEG C, periodicity of illumination is bright phase 17h, dark phase 7h environment
In, carry out germination culture；The seedling-erecting ratio of the 8th day seed for counting each rape variety, select preceding 20% high kind of seedling-erecting ratio
Rape variety corresponding to son is primary dcreening operation rape variety, i.e., oil grinds No. 10, Feng is oily No. 737, China's oil is miscellaneous No. 62, moral oil No. 5 etc. 30
Rape variety；
F. cadmium nitrate and ultra-pure water are utilized, cadmium solution is prepared, then takes 30 groups of Culture basins, be designated as the 1st group~the 30th group,
Every group of 8 Culture basins, label 1~8, every group is taken 7 parts of soil, sprays cadmium solution respectively into per a soil, and mixing is equal
Even, the cadmium solution concentration for making 7 parts of soil is respectively 30mg/kg, 50mg/kg, 70mg/kg, 90mg/kg, 110mg/kg, 130mg/
Kg, 150mg/kg, the soil for being then uniformly mixed with 7 parts cadmium solution is fitted into 7 Culture basins, according to 30mg/kg, 50mg/
Kg, 70mg/kg, 90mg/kg, 110mg/kg, 130mg/kg, 150mg/kg concentration order are fitted into 1~No. 7 Culture basin, then
1 part of soil is taken, sprays isometric aqueous solution thereto, is then charged into No. 8 Culture basins, as a control group；
G., the seed that oil is ground to 30 kinds of No. 10 grades is all divided into 8 parts, every part of 45 seeds, in conventional seed planting season,
It is seeded in the 1st group~the 30th group of Culture basin, each Culture basin is applied fertilizer respectively then, fertilizer and the soil in Culture basin
Mass ratio is 5:8000, then field management is carried out, until seed is all grown to rapeseed plants, then determine the soil in each Culture basin
The cadmium content of root and the cadmium content of plant shoot of rapeseed plants corresponding to the cadmium content of earth, every part of soil, according to soil
The selection standard of front and rear cadmium content contrast super enrichment rape is administered, learns that Feng oil 737, the kind of moral oil 5 make in soil
Cadmium content reduce, and meet the selection standard of super enrichment rape, be defined as super enrichment rape variety.
Embodiment three
Embodiments of the invention three provide a kind of super enrichment heavy metal foliage filter screening method, comprise the following steps,
A. 400 double No. 11 seeds in selecting, using the alcohol-pickled seed 5min that mass concentration is 75%, wine is poured out
Essence, seed 10min is soaked followed by 5g/kg sodium hypochlorite, pours out sodium hypochlorite, then with pure water by wash seeds 3 times,
Cleaned 1 time with ultra-pure water again；
B. the hydrate of caddy 2/5 and pure water are utilized, sets up 10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/
The cadmium test solution of 7 concentration gradients of kg, 100mg/kg, 120mg/kg；
C. 400 rape seeds are divided into 8 parts, are individually positioned in 8 culture dishes for being lined with 2 layers of filter paper, compile successively
Number be 1~No. 8, each 50 rape seeds of culture dish, wherein it is 10~120mg/ that 4ml concentration, which is added dropwise, in 1~No. 7 culture dish successively
Kg cadmium test solution, retains the clear water that equivalent is added dropwise in No. 8 culture dishes, as a control group, sets up 2 repetitions；
D. step c all culture dishes are placed in 23 DEG C, periodicity of illumination is bright phase 16h, in dark phase 8h environment, is sent out
Bud culture, the 7th day statistics germination percentage and seedling-erecting ratio, the concentration for choosing the cadmium test solution that No. 5 culture dishes are added dropwise is screening criteria
Concentration, i.e. 80mg/kg；
E. miscellaneous No. 10 moral oil, southern oily miscellaneous No. 1, magnificent Anhui oil 2, section's pleasure 1, Deyou 8, moral oil 5 magnificent oily miscellaneous 14 are selected
Number, China's oil is miscellaneous No. 16, Hunan is miscellaneous No. 695, Hunan oil 17, in it is double No. 4, in it is double No. 8, in double 200 rape varieties such as No. 11, wherein
Including 156 conventional varieties (homozygous kind) and 44 Hybrids, each 50 seeds of variety adoption；
F. all seeds of step e all kinds are placed at 28 DEG C and shine 24h, all seeds are then used into step
The alcohol-pickled method to ultra-pure water cleaning is pre-processed in a；The seed of pretreated 200 rape varieties is put into again
It is lined with 200 culture dishes of 2 layers of filter paper, the screening criteria concentration that 4ml steps d confirms is added dropwise in each culture dish, sets up control
With 2 repetitions, then all culture dishes are placed in 23 DEG C, periodicity of illumination is bright phase 16h, in dark phase 8h environment, carries out germination training
Support；The germination percentage and seedling-erecting ratio of the 7th day seed for counting each rape variety, using germination percentage as with reference to while select it is straight
Rape variety corresponding to preceding 10% high seed of seedling rate is primary dcreening operation rape variety, as Hunan oil 15, China it is double No. 3, in it is double No. 11,
Moral oil 20 kinds of No. 5 grades；
F. utilize the hydrate of caddy 2/5 and pure water, prepare cadmium solution, then take 20 groups of Culture basins, be designated as the 1st group~
20th group, every group of 7 Culture basins, label 1~7；
Every group is taken 6 parts of soil, every part of soil first pass through it is air-dried, sort, pick, be levigate, sieving is dried, crushed, sieving
Processing, cadmium solution is then sprayed respectively into per a soil, is well mixed, the cadmium solution concentration for making 6 parts of soil is respectively
10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg, then uniformly it is mixed with cadmium solution by 6 parts
Soil is fitted into 6 Culture basins, according to the dense of 10mg/kg, 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg
Degree order is fitted into 1~No. 6 Culture basin, then takes 1 part of soil, sprays isometric aqueous solution thereto, is then charged into No. 7 trainings
Support in basin, as a control group, 2 repetitions are set；
G. the seed of 20 kinds of double No. 11 grades is all placed in shining 24h at 28 DEG C by, then in conventional seed planting season, point
It is not seeded in the 1st group~the 20th group of Culture basin, then each Culture basin is applied fertilizer, the matter of fertilizer and the soil in Culture basin
Amount is than being 5:7000, nitrogenous 15% in fertilizer, it is phosphorous 15%, containing potassium 15%；
Field management is carried out again, until seed is all grown to rapeseed plants, the soil in each Culture basin is determined before bolting
The cadmium content of the cadmium content of the root of rapeseed plants corresponding to the cadmium content of earth, every part of soil and plant shoot (stem, leaf),
Determined again after blossoming and having seeds the cadmium content of the soil in each Culture basin, rapeseed plants corresponding to every part of soil root cadmium
The cadmium content for the seed that content and plant shoot (stem, leaf), rape are born, contrasted according to the cadmium content before and after soil remediation super
The selection standard of rape is enriched with, learns that oily No. 5 kinds of moral reduce the cadmium content in soil, and meet the choosing of super enrichment rape
Standard is taken, is defined as super enrichment rape variety.
In the present embodiment, cadmium detection accepted standard：Root and stalk accepted standard are GB/T 13082-91 AAS, soil
Earth accepted standard is US EPA METHOD, and seed accepted standard is GB5009.15-2014 GFAAS, determines that rape is super rich
Collection ability.
In the prior art, 200m is typically utilized2Greenhouse carry out potted plant, cost is high, it is necessary to certain floor space, management
High, it is necessary to which planting for 3 generations can just complete to screen with screening cost, the cycle needs 2~3 years.And the side for utilizing the present embodiment to provide
Method is screened, and the cycle below 2 years, reduces by more than 70% in management and in cost.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (11)
1. a kind of screening technique of the kind of super enrichment huge sum of money platymiscium, it is characterised in that comprise the following steps：
Set screening criteria concentration：The more seeds of plant by pretreatment are respectively put into the different multiple heavy metals of concentration
Test in solution, carry out germination culture, choose the Heavy metal solution where the seed for the plant that seedling-erecting ratio is 50%
Concentration be the screening criteria concentration, the seedling-erecting ratio=(B/C) X 100%, B is upright seedling number, and C is germination number；
Choose the kind of primary dcreening operation plant：From multiple kinds of the plant, by the kind of each kind by pretreatment
Son, it is put into the heavy metal screening solution with the screening criteria concentration, carries out germination culture, calculate each kind
The seedling-erecting ratio of seed, the kind corresponding to preceding 10~20% high seed of seedling-erecting ratio is selected as the primary dcreening operation plant
Kind；And
Screen the kind of super enrichment huge sum of money platymiscium：The seed of the kind of each primary dcreening operation plant by pretreatment is distinguished
It is seeded in more parts of different soil of heavy metal concentration, and carries out field management, until it is grown to plant, determines every part
The content of beary metal of the root of the plant corresponding to the content of beary metal of the soil and every part of soil and plant
The content of beary metal on top, when plant survival, and the content of beary metal of its plant shoot is higher than the weight of root
Tenor, and the substance withdrawl syndrome of its plant shoot is more than the substance withdrawl syndrome of root, and the soil where it
When content of beary metal before earth improvement is more than the content of beary metal after administering, it is super rich to determine the kind belonging to the plant
Collect the kind of huge sum of money platymiscium.
2. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that in the choosing
The seed of each plant variety is pre-processed in the step of taking the kind of primary dcreening operation plant to be included：Seed is dried in the air at 20~35 DEG C
Shine 20~30h；Each primary dcreening operation plant variety is pre-processed in the step of screening super enrichment heavy metal plant variety
The seed includes：The seed is dried into 20~30h at 20~35 DEG C.
3. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that the plant
For rape, the heavy metal is cadmium.
4. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 3, it is characterised in that the huge sum of money
Category solution is at least one of the hydrate soln of caddy 2/5, nitric acid cadmium solution.
5. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 4, it is characterised in that the huge sum of money
Category solution is the hydrate soln of caddy 2/5.
6. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that in the sieve
In the step of selecting the kind of super enrichment huge sum of money platymiscium, the heavy metal concentration of the more parts of soil is 0.3~150mg/kg.
7. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that in the sieve
In the step of selecting the kind of super enrichment huge sum of money platymiscium, measure content of beary metal is further：Taken out respectively in the plant
Before tongue and after blossoming and having seeds, the plant corresponding to the content of beary metal and every part of soil of every part of soil is determined respectively
The content of beary metal of the root of thing plant and the content of beary metal of plant shoot.
8. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 7, it is characterised in that in the plant
After thing plant blossom is set seeds, the heavy metal for the seed that the plant corresponding to determining every part of soil bears also is needed to contain
Amount.
9. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that in the sieve
In the step of selecting the kind of super enrichment huge sum of money platymiscium, after the sowing, before the field management is carried out, also wrap
Include：To soil application fertilizer every part described, the mass ratio of the fertilizer and the soil is 5:7000~8000.
10. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 9, it is characterised in that the fertilizer
In material, nitrogen content is 12~18%, and phosphorus content is 12~18%, and potassium content is 12~18%.
11. the screening technique of the kind of super enrichment huge sum of money platymiscium according to claim 1, it is characterised in that described
In the step of kind of the step of setting screening criteria concentration and the selection primary dcreening operation plant, the condition of the germination culture is equal
For：22~25 DEG C of temperature, periodicity of illumination are bright 15~17h of phase, dark 7~9h of phase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610250906.8A CN105750212B (en) | 2016-04-20 | 2016-04-20 | A kind of screening technique of the kind of super enrichment huge sum of money platymiscium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610250906.8A CN105750212B (en) | 2016-04-20 | 2016-04-20 | A kind of screening technique of the kind of super enrichment huge sum of money platymiscium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105750212A CN105750212A (en) | 2016-07-13 |
| CN105750212B true CN105750212B (en) | 2018-03-27 |
Family
ID=56325398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610250906.8A Active CN105750212B (en) | 2016-04-20 | 2016-04-20 | A kind of screening technique of the kind of super enrichment huge sum of money platymiscium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105750212B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107241981B (en) * | 2017-05-19 | 2021-04-13 | 贵州省蚕业研究所 | Method for screening cadmium-resistant or low-cadmium excellent germplasm resources of capsicum |
| CN109892175B (en) * | 2019-01-09 | 2021-11-05 | 云南省农业科学院经济作物研究所 | Identification method of lead-resistant industrial hemp germplasm resource |
| CN110918639A (en) * | 2020-01-17 | 2020-03-27 | 湖南超富集生态科技有限公司 | Method for repairing cadmium-polluted soil by using activation of Sedum plumbizincicola |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6313374B1 (en) * | 1997-11-04 | 2001-11-06 | University Of Guelph | Method of using pelarogonium sp. as hyperaccumulators for remediating contaminated soil |
| CN1568668A (en) * | 2003-07-16 | 2005-01-26 | 中国科学院沈阳应用生态研究所 | Screening method for heavy metal enriched / accumulated plant |
| CN101743905A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for selecting crop varieties with low heavy metal accumulation |
| CN102246642A (en) * | 2011-04-11 | 2011-11-23 | 黄石理工学院 | Method for screening heavy metal hyperaccumulator by using mining wasteland |
| CN103639183A (en) * | 2013-12-20 | 2014-03-19 | 湖南农业大学 | Method for restoring heavy-metal-contaminated soil by utilizing oil sunflower planting |
| CN104984987A (en) * | 2015-06-23 | 2015-10-21 | 沈阳大学 | Method for screening lead/cadmium repair tolerant plants from metal smelting region |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7214516B2 (en) * | 2004-04-01 | 2007-05-08 | University Of Maryland | Bacterial effects on metal accumulation by plants |
-
2016
- 2016-04-20 CN CN201610250906.8A patent/CN105750212B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6313374B1 (en) * | 1997-11-04 | 2001-11-06 | University Of Guelph | Method of using pelarogonium sp. as hyperaccumulators for remediating contaminated soil |
| CN1568668A (en) * | 2003-07-16 | 2005-01-26 | 中国科学院沈阳应用生态研究所 | Screening method for heavy metal enriched / accumulated plant |
| CN101743905A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for selecting crop varieties with low heavy metal accumulation |
| CN102246642A (en) * | 2011-04-11 | 2011-11-23 | 黄石理工学院 | Method for screening heavy metal hyperaccumulator by using mining wasteland |
| CN103639183A (en) * | 2013-12-20 | 2014-03-19 | 湖南农业大学 | Method for restoring heavy-metal-contaminated soil by utilizing oil sunflower planting |
| CN104984987A (en) * | 2015-06-23 | 2015-10-21 | 沈阳大学 | Method for screening lead/cadmium repair tolerant plants from metal smelting region |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105750212A (en) | 2016-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107066823B (en) | Based on plant, soil, microorganism heavy-metal contaminated soil repairing effect integrated evaluating method | |
| Wei et al. | Effect of fertilizer amendments on phytoremediation of Cd-contaminated soil by a newly discovered hyperaccumulator Solanum nigrum L. | |
| CN104529668B (en) | Metal mine soil conditioner, and mine ecological remediation method using metal mine soil conditioner. | |
| Xiu-Zhen et al. | Growth, cadmium and zinc accumulation of ornamental sunflower (Helianthus annuus L.) in contaminated soil with different amendments | |
| CN101743905B (en) | Method for selecting crop varieties with low heavy metal accumulation | |
| Kolbas et al. | Copper phytoextraction in tandem with oilseed production using commercial cultivars and mutant lines of sunflower | |
| CN107695088A (en) | The method that As Cd combined pollution agricultural land soils are repaired using super enriching plant biomass carbon combined system | |
| CN101393191B (en) | Screening method for heavy metal low accumulated and low enriched vegetable variety | |
| Zhong et al. | Effect of continuous cropping on soil chemical properties and crop yield in banana plantation. | |
| CN103480643A (en) | Method for restoring cadmium/lead single or combined polluted soil by using hyperaccumulating flower emilia sonchifolia | |
| CN105750212B (en) | A kind of screening technique of the kind of super enrichment huge sum of money platymiscium | |
| Esringü et al. | The roles of diethylenetriamine pentaacetate (DTPA) and ethylenediamine disuccinate (EDDS) in remediation of selenium from contaminated soil by Brussels sprouts (Brassica oleracea var. gemmifera) | |
| CN102246642A (en) | Method for screening heavy metal hyperaccumulator by using mining wasteland | |
| CN108817048A (en) | A method of Cd contaminated soil is repaired using work between herbaceous plant and water spinach | |
| Shallari et al. | Availability of nickel in soils for the hyperaccumulator Alyssum murale Waldst. & Kit. | |
| CN108393338B (en) | Method for repairing metal strontium polluted soil by reinforced ryegrass and application | |
| Phiri et al. | Plant growth, mycorrhizal association, nutrient uptake and phosphorus dynamics in a volcanic-ash soil in Colombia as affected by the establishment of Tithonia diversifolia | |
| CN104607444A (en) | Method for restoring Cr-polluted soil by utilizing cyperus rotundus L. | |
| CN101912872B (en) | Method for restoring cadmium contaminated soil by using cypress seedlings | |
| CN103238439B (en) | Method for screening cadmium rejection varieties of leaf-edible rapes | |
| Li et al. | Soil boron content and the effects of boron application on yields of maize, soybean, rice and sugarbeet in Heilongjiang Province, PR China | |
| CN105149333A (en) | Method for restoring strontium-contaminated soil through oilseed rape | |
| Yang et al. | Variations of growth, nitrogen accumulation and nitrogen use efficiency among 18 willow clones under two nitrogen regimes | |
| CN107117714A (en) | A kind of method of vegetable plant floating bed purifying city urban river water | |
| Da Silva et al. | Screening maize inbred lines for tolerance to low-P stress condition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |