CN105749278A - Porcine pseudorabies-preventing composition, preparation method and application - Google Patents
Porcine pseudorabies-preventing composition, preparation method and application Download PDFInfo
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Abstract
The invention provides a porcine pseudorabies-preventing composition, a preparation method and application, and relates to the field of feed additives. The porcine pseudorabies-preventing composition comprises 75-85 parts of a traditional Chinese medicine composition and 15-25 parts of a Western medicine composition, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 30-40 parts of the root of Chinese pulsatilla, 30-40 parts of uncaria rhynchophylla, 15-20 parts of kudzuvine roots, 15-20 parts of pine needles, 20-30 parts of fructus forsythia, 25-35 parts of honeysuckle and 6-10 parts of gynostemma pentaphylla; the Western medicine composition is prepared from the following components in parts by volume in manners of mixing and depressurized drying: 75-85 parts of a porcine pseudorabies-preventing egg yolk antibody, 15-25 parts of a porcine pseudorabies-preventing transfer factor and 0.2-0.3 part of an inactivator. The invention further provides a preparation method of the porcine pseudorabies-preventing composition and feed containing the porcine pseudorabies-preventing composition. The porcine pseudorabies-preventing composition provided by the invention is used for preventing and treating porcine pseudorabies and is relatively high in protection rate.
Description
Technical field
The present invention relates to feed additive field, be specifically related to the composition of a kind of resisting pstudorabies, preparation method and application.
Background technology
Pseudorabies virus (Pseudorabies virus, PRV) belongs to herpetoviridae (Herpesviridae), herpesvirus suis belongs to,
Virion is circular, diameter 150～180nm, nucleocapsid a diameter of 105～110nm.The host that PRV infects
Spectrum is wide, including pig, ox, sheep, mouse, rabbit, cat, dog etc..Pig is the unique species can survived after PRV infects, because of
This is also the reservoir host of this virus.Present after other host infection in addition to pig and very itch, generate heat and nervous symptoms, and finally
It is attributed to death.This means that if purifying this virus from swinery will cause the thorough elimination of this disease.PRV can
Vertical transmission, again can horizontal transmission.And this virus once infects, lifelong band poison, under certain conditions, the disease of latence
Poison can be activated, and causes recurrent infection and to external diffusion.
Since 2011, porcine pseudorabies virus variant is widely current in China, causes serious economic loss.Lactation is young
The pig incidence of disease is up to 100%, and the death rate is up to about 95%；The weanling pig performance incidence of disease is 20%～40%, and the death rate can
Reach about 30%；Adult Pig appearance is vomitted, suffers from diarrhoea, is coughed, and with death；Produce sow show as sterile, return feelings rate high,
Repeat breeding.The large quantities of miscarriage of in-pig, produces stillborn foetus or mummy tire.This time no matter the maximum feature of porcine pseudorabies epidemic situation is
The traditional pseudorabies vaccine of immunity again without immunity, all has morbidity in various degree.
Vaccine immunity remains the important measures of preventing and treating pseudorabies at present.But after traditional vaccine immunity, swinery still has higher
The incidence of disease, and relatively low to the protective rate of new variation strain, preventing and treating and elimination to this disease cause obstacle.
Summary of the invention
It is an object of the invention to overcome above-mentioned weak point, it is provided that the composition of a kind of resisting pstudorabies, may be used for prevention
With treatment porcine pseudorabies, the highest to tradition strain and the protective rate of new variation strain.
It is a further object of the present invention to provide the preparation method of the composition of resisting pstudorabies, the method is simple, safety, cost
Low.
Another object of the present invention is to provide the feed containing described resisting pstudorabies composition, may be used for prevention and treatment pig
Pseudoabies is higher to the protective rate of tradition strain and new variation strain.
The composition of a kind of resisting pstudorabies, the composition of described resisting pstudorabies includes the Chinese medicine composition of 75-85 weight portion
Western medicine compound with 15-25 weight portion；
Described Chinese medicine composition is made up of the Chinese medicine material of following weight portion: Chinese bulbul 30-40 part, yncaria stem with hooks 30-40 part, the root of kudzu vine
15-20 part, pine needle 15-20 part, capsule of weeping forsythia 20-30 part, honeysuckle 25-35 part, gynostemma pentaphylla 6-10 part；
Described Western medicine compound is the component mixing of volumes below part, makes after drying under reduced pressure: vitelline antibody for resisting pstudorabies
75-85 part, resisting pstudorabies transfer factor 15-25 part, inactivator 0.2-0.3 part.
In the present invention, described vitelline antibody for resisting pstudorabies is adopted and is prepared with the following method: use swine pseudorabies vaccine
Immune health laying hen, collects height and exempts from egg, take yolk, mix with water, obtain vitelline antibody for resisting pstudorabies；Institute
The mass ratio stating yolk and water is 1:2-4；Described resisting pstudorabies transfer factor is adopted and is prepared with the following method: takes immunity and crosses pig puppet
The spleen of the pig of rabies live vaccine, adds the water of pig spleen volume 1.5-2.5, after homogenate, multigelation, regulates pH to 6.5-7.0,
Centrifugal, take supernatant, obtain resisting pstudorabies transfer factor.
Preferably in technical scheme, the composition of described resisting pstudorabies includes the Chinese medicine composition of 80 weight portions and 20 parts
Western medicine compound.
Preferably in technical scheme, described Chinese medicine composition is made up of the Chinese medicine material of following weight portion: the Chinese bulbul 35 parts, yncaria stem with hooks
35 parts；The root of kudzu vine 18 parts, 18 parts of pine needle, the capsule of weeping forsythia 25 parts, honeysuckle 30 parts, gynostemma pentaphylla 8 parts；Described Western medicine compound
It is the freeze-dried powder made of drying under reduced pressure after the component mixing of volumes below part: vitelline antibody for resisting pstudorabies 80 parts, anti-pig puppet is mad
Dog disease transfer factor 20 parts, inactivator 0.2-0.3 part.
In the present invention, described inactivator is 37-40% formalin.
The present invention also provides for the preparation method of described resisting pstudorabies composition, comprises the following steps:
(1) Chinese medicine composition is prepared: take the Chinese bulbul, yncaria stem with hooks, the root of kudzu vine, pine needle, the capsule of weeping forsythia, honeysuckle and gynostemma pentaphylla and be separately dried,
Grinding and sieving, mixes, and obtains described Chinese medicine composition；
(2) Western medicine compound is prepared；
(3) Western medicine compound is mixed with described Chinese medicine composition, obtain described resisting pstudorabies composition.
In the present invention, described Western medicine compound is adopted and is prepared with the following method: use swine pseudorabies vaccine immune health to produce
Laying hen, collects height and exempts from egg, take yolk, mix with water, obtain vitelline antibody for resisting pstudorabies；Described yolk and water
Mass ratio be 1:2-4；Take the spleen that the pig of pseudorabies disease live-vaccine is crossed in immunity, add the water of pig spleen volume 1.5-2.5 times,
After homogenate, multigelation, regulate pH to 6.5-7.0, centrifugal, take supernatant, obtain resisting pstudorabies transfer factor；Will be anti-
Porcine pseudorabies Yolk antibody, resisting pstudorabies transfer factor and inactivator mixing, filtration sterilization, obtain institute after drying under reduced pressure
State Western medicine compound.
The present invention also provides for the feed containing described resisting pstudorabies composition.
Preferably in technical scheme, in described feed, the mass percent of resisting pstudorabies composition is 0.15%-0.7%.
The each Chinese medicine material characteristic that the present invention relates to is as follows:
The Chinese bulbul: anti-oxidant, anti-inflammatory, cool blood, heat-clearing, removing toxic substances.Cure mainly toxic-heat and blood stasis, a kind of malaria, the diseases such as blood is defeated in battle, hemorrhoid hemorrhage.
Yncaria stem with hooks: calm, hypotensive effect.Curing mainly the flat liver of heat-clearing, relieving convulsion and spasm, heat contains dynamic wind, eclampsia, dizziness due to hyperactivity of liver-YANG, irascibility
Distending pain in the head.
The root of kudzu vine: expelling pathogenic factors from muscles and skin is brought down a fever, promoting eruption, promote the production of body fluid to quench thirst, rise sun antidiarrheal, generate heat for table card, stiff nape and back, measles without adequate eruption,
Pyreticosis is thirsty, and the deficiency of Yin is quenched one's thirst, and heat is purged heat dysentery, splenasthenic diarrhea.
Pine needle: control expelling wind and activating blood flow, improving eyesight, calm the nerves, removing toxic substances, antipruritic.
The capsule of weeping forsythia: function cures mainly heat-clearing, removing toxic substances, dissipating bind, detumescence.Control warm, erysipelas, macula, large carbuncle pyogenic infections, scrofula.
Honeysuckle: there is effect of bloodization silt clearing heat and detoxicating, cool, cure mainly affection of exogenous wind-heat, seasonal febrile diseases from the beginning of, sore serious case of furuncle, redness
Heat pain, passing stool with pus and blood " etc..
Gynostemma pentaphylla: replenish qi to invigorate the spleen, preventing phlegm from forming and stopping coughing, clearing heat and detoxicating, cure mainly: body void is weak；Nephrasthenia emission；Leukopenia；
Hyperlipidemia；Virus hepatitis；Chronic gastroenteritis；Chronic bronchitis.
The composition of resisting pstudorabies of the present invention, may be used for prevention and treatment porcine pseudorabies, to tradition strain and new variation
The protective rate of strain is the highest.When being added with the 400 or 600g present composition in 100kg feed, protective rate is up to 100%.
Finding through overtesting, when individually adding Chinese medicine composition or Western medicine compound in feed, protective rate is less than adding the present invention simultaneously
The protective rate of composition.The present composition is used to have only to add in feed, it is to avoid to need to catch pig during vaccine inoculation,
Expend a large amount of manpower, use more convenient, efficient.The preparation method of the present composition is simple, safety, low cost.
Detailed description of the invention
The composition A of embodiment 1 resisting pstudorabies
The composition A of resisting pstudorabies, including Chinese medicine composition and the Western medicine compound of 20 weight portions of 80 weight portions.Tool
Preparation is as follows:
(1) Chinese medicine composition is prepared
Following Chinese medicine material is weighed respectively: the Chinese bulbul 35 parts, yncaria stem with hooks 35 parts, the root of kudzu vine 18 parts, pine needle 18 according to weight portion
Part, the capsule of weeping forsythia 25 parts, honeysuckle 30 parts, gynostemma pentaphylla 8 parts.Each Chinese medicine material is separately dried, pulverizes, cross 700 mesh sieve,
Take the Chinese medicine material powder under sieve.Chinese medicine material powder under sieve is mixed, obtains Chinese medicine composition.
(2) Western medicine compound is prepared
Western medicine compound is that the component of volumes below number mixes, and makes after drying under reduced pressure: resisting pstudorabies yolk resists
Body 80 parts, resisting pstudorabies transfer factor 20 parts, 40% formalin 0.2-0.3 part.
Vitelline antibody for resisting pstudorabies preparation method is as follows: (pseudorabies virus makes a variation in 1. pseudorabies virus AH02LA strain
Strain, derives from National Research Center of Veterinary Biologicals Engineering and Technology, is disclosed in the application for a patent for invention of Application No. 2015103883909),
Employing conventional method is bred, formalin-inactivated, and inactivated vaccine, every 1ml viral level 107.1 TCID50 are made in white-oil adjuvant emulsification；
2. taking step 1. inactivated vaccine 1mL intramuscular injection immune health laying hen, head is spaced after exempting from and exempted from two Tuesdays, two exempt from after be spaced two weeks
Three exempt from, each 1mL；3. two exempt from after and three exempt from after 1 week wing venous blood collection, separate serum, carry out serum neutralization test detect pig
Pseudorabies virus antibody titer (Yang Wenping, Gu Zhenqing, Sun Haifeng, etc. the antigenicity of the popular strain of Pseudorabies virus and serum
Neutralize specificity analysis [J]. herding and animal doctor, 2014, (10): 11-14.), when antibody titer is more than or equal to 1:256, collects height and exempt from
Egg；4. being cleaned up by collected high-immunity egg, eggshell surface is sterilized, and is dried, puts in superclean bench, aseptic collects egg
Yellow.Adding the sterile purified water of its quality 3 times in the yolk collected, stir, 4 DEG C stand 12h, use filter pump
Reclaim supernatant, obtain vitelline antibody for resisting pstudorabies.
Resisting pstudorabies transfer factor preparation method is as follows: slaughterhouse selects at least intramuscular injection porcine pseudorabies virus live vaccine (to contain
Have Bartha-k61 strain, purchased from Cimmeria animal health Co., Ltd or Shuo Teng animal health-care product Co., Ltd) growing and fattening pigs of twice,
Take its spleen, spleen is put in sterile saline, remove surface manadesma and knot hoof tissue, cleaner with normal saline flushing.
Adding the sterilizing deionized water of its 2 times of volumes in gained spleen, homogenate, homogenate sonicator processes 10min, broken
Homogenate pH value after freeze thawing ,-40 DEG C of multigelations 3 times, is finally transferred to 6.8 by broken liquid, and at 4 DEG C, 8000r/min is centrifuged 30min,
Collect supernatant and be resisting pstudorabies transfer factor.
Vitelline antibody for resisting pstudorabies, resisting pstudorabies transfer factor, inactivator are mixed according to above-mentioned volume parts,
Degerming through 0.45 μm and 0.1 μm membrane filtration successively, under the conditions of 50-56 DEG C, carry out drying under reduced pressure make dry powder, obtain Western medicine
Composition.
(3) mixing
Count by weight, accurately weigh Chinese medicine composition 80 parts, Western medicine compound 20 parts, mix, obtain anti-pig pseudo-
Rabic composition A.
The composition B of embodiment 2 resisting pstudorabies
The composition B of resisting pstudorabies, including the component of following parts by weight: Chinese medicine composition 75 parts, Western medicine compound 25
Part.Wherein, the preparation method of Chinese medicine composition is with embodiment 1.
Western medicine compound is the component mixing of volumes below number, makes: vitelline antibody for resisting pstudorabies 75 after drying under reduced pressure
Part, resisting pstudorabies transfer factor 25 parts, inactivator (40% formalin) 0.2-0.3 part.Resisting pstudorabies yolk
The preparation method of antibody and resisting pstudorabies transfer factor is with embodiment 1.By vitelline antibody for resisting pstudorabies, anti-pseudorabies
Sick transfer factor, inactivator mix, degerming through 0.45 μm and 0.1 μm membrane filtration successively, enter under the conditions of 50-56 DEG C
Row drying under reduced pressure makes dry powder, obtains Western medicine compound.
Count by weight, accurately weigh Chinese medicine composition 75 parts, Western medicine compound 25 parts, mix, obtain anti-pig pseudo-
Rabic composition B.
The composition C of embodiment 3 resisting pstudorabies
The composition C of resisting pstudorabies, including the component of following parts by weight: Chinese medicine composition 85 parts, Western medicine compound 15
Part.
Wherein, the preparation method of Chinese medicine composition is with embodiment 1.
Western medicine compound is the component mixing of volumes below number, makes: vitelline antibody for resisting pstudorabies 85 after drying under reduced pressure
Part, resisting pstudorabies transfer factor 15 parts, inactivator (40% formalin) 0.2-0.3 part.Resisting pstudorabies poison ovum
The preparation method of yellow antibody and resisting pstudorabies transfer factor is with embodiment 1.By mad to vitelline antibody for resisting pstudorabies, anti-pig puppet
Dog disease transfer factor, inactivator mix, degerming, under the conditions of 50-56 DEG C through 0.45 μm and 0.1 μm membrane filtration successively
Carry out drying under reduced pressure and make dry powder, obtain Western medicine compound.
Count by weight, accurately weigh Chinese medicine composition 85 parts, Western medicine compound 15 parts, mix, obtain anti-pig pseudo-
Rabic composition C.
Experimental example 4
The composition A (being abbreviated as composition A) of the resisting pstudorabies of preparation in embodiment 1 is added to feed, checking group
Poison Vaccine effectiveness is attacked in porcine pseudorabies virus AH02LA strain (pseudorabies virus variant) by compound A.
No matter whether adding present composition A in feed, the feed mode of each group piglet is free choice feeding, 24h not fracture.
Selecting 140 pseudorabies feminine gender piglets, be randomly divided into 7 groups, often group 20, packet is described as follows:
G1 group (prophylactic group): composition A is added and feeds G1 group test pig, composition A to commercially available conventional feed
Addition is that every 100kg feed adds 200g composition A.After composition A is used in conjunction 5 days, use porcine pseudorabies virus
Poison is attacked in AH02LA strain (10 7.1TCID50), observes incidence.
G2 group (therapeutic administratp group A): use porcine pseudorabies virus AH02LA strain (107.1TCID50) poison G2 group examination is attacked
Testing pig, and used composition A feeding experiment pig the same day in attacking poison, composition A addition is that the commercially available conventional feed of every 100kg adds
Add 200g composition A.After being used in conjunction 5 days, observe incidence.
G3 group (therapeutic administratp group B): use porcine pseudorabies virus AH02LA strain (107.1TCID50) poison G3 group examination is attacked
Testing pig, and used composition A feeding experiment pig the same day in attacking poison, composition A addition is that the commercially available conventional feed of every 100kg adds
Add 400g composition A.It is used in conjunction 5 days, observes incidence.
G4 group (therapeutic administratp group C): use porcine pseudorabies virus AH02LA strain (107.1TCID50) poison G4 group examination is attacked
Testing pig, and used composition A feeding experiment pig the same day in attacking poison, composition A addition is that the commercially available conventional feed of every 100kg adds
Add 600g composition A.After being used in conjunction 5 days, observe incidence.
G5 group (vaccine control group): G5 group test pig feeds commercially available conventional feed, commercially available tradition pseudorabies live vaccine (Wuhan
Zhong Bo Biological Co., Ltd., containing porcine pseudorabies virus Bartha K-61 strain) intramuscular injection immunity, every pig 1ml,
After immunity 14 days, use porcine pseudorabies virus AH02LA strain (107.1TCID50) attack poison, observe incidence.
G6 group (attacks poison control group): G6 group test pig feeds commercially available conventional feed., use porcine pseudorabies virus AH02LA strain
(107.1TCID50) attack poison, observe incidence.
G7 group (negative control group): G7 group test pig feeds commercially available conventional feed, does not attack poison, observes incidence.
Clinical onset situation is observed: porcine pseudorabies virus AH02LA strain (107.1TCID50) viewing test pig fever after poison is attacked,
Nasal mucus, the clinical symptoms such as nervous symptoms, calculate the incidence of disease, the death rate and protective rate, observe number of days for attacking malicious latter 10 days.
Incidence of disease computational methods: the incidence of disease=affected animal number/experimental animal number × 100%.
Death rate computational methods: the death rate=dead animal number/experimental animal number × 100%.
Protective rate calculation: without clinical symptoms number of animals/experimental animal number × 100% after protective rate=off-test.
Table 1 respectively organizes experiment pig morbidity and death condition statistical form
Each group experiment pig morbidity is shown in Table 1 with death condition.Every 100kg feed adds 400g or 600g combination as can be seen from Table 1
During thing A, porcine pseudorabies virus variant AH02LA strain is had the protective rate of 100%.Every 100kg feed adds 400g
And combination of the above thing A, can effectively cure porcine pseudorabies as treatment consumption.Feed adds before attacking poison composition A, also
Prevention porcine pseudorabies there is extraordinary effect.
Embodiment 5
Selecting 25 age in days pseudorabies feminine gender piglet 75, be randomly divided into 5 groups, often group 15, each group profile is as follows:
G1 group (Chinese medicine composition group): G1 group piglet feeds the Chinese medicine composition of preparation in embodiment 1, and every 100kg is commercially available often
Rule feed adds 320g Chinese medicine composition.
G2 group (Western medicine compound group): G2 group piglet feeds the Western medicine compound of preparation in embodiment 1, in every 100kg feed
Western medicine compound 80g.
G3 group (composition A group): G3 group piglet feed composition A, every 100kg feed adds 400g composition A (containing real
Execute Chinese medicine composition 320g Western medicine compound 80g in example 1).
G4 group (attacks poison comparison): G4 group piglet only attacks poison, not medicine feed.
G5 group (negative control): G5 group piglet not medicine feed, does not attack poison
Wherein, G1, G2, G3 and G4 group attacks poison porcine pseudorabies virus AH02LA strain (107.1TCID50), poison mode is attacked
For collunarium 1 milliliter, intramuscular injection 1 milliliter.After attacking poison, the same day starts to feed as described above, observes 14 days, calculates morbidity number,
Death toll, the statistics death rate and protective rate.
The result of medication treatment after poison is attacked in table 2 pseudorabies virus AH02LA strain
The most individually Chinese medicine composition or individually Western medicine compound, make a variation to pseudorabies virus
Strain AH02LA strain all has certain protective effect, but protective effect is limited, and protective rate is respectively less than 60%, but both share, can
Increasing its protected effect to pseudorabies virus variant AH02LA strain, protective rate reaches 100%.Result illustrates this invention
Composition, compares independent Chinese medicine or independent Western medicine compound, and treatment porcine pseudorabies effect is more notable.
Embodiment 6
This example demonstrates that composition A prepared by embodiment 1 is the highest to tradition strain and new variant protective rate.
Selecting 24 20 age in days pseudorabies feminine gender piglets at random, be randomly divided into 4 groups, G1 and G2 group immunity pseudorabies passes
System Bartha-K61 vaccine (purchased from Wuhan Chopper Biology Co., Ltd.), G3 and G4 is the most immune.G1 and G3 after 28 days
Group attacks poison PRV PRV-JS strain (tradition strain, be disclosed in the patent of invention of Patent No. 201210467482.2), attacks poison mode
For collunarium 1 milliliter, intramuscular injection 1 milliliter, every milliliter of viral level is 107.1TCID50；G2 and G4 group attacks poison pseudoabies
Virus AH02LA strain, attacking poison mode is collunarium 1 milliliter, and intramuscular injection 1 milliliter, every milliliter of viral level is 107.1TCID50。
Attacking the malicious same day, G3 and G4 group feed composition A, every 100kg feed adds 400g composition A, is used in conjunction 14 days, the phase
Between observe each group of M & M.Result is as shown in table 3, and no matter composition A is to tradition strain or to variant, all
There is preferably protection, without morbidity and death.Traditional vaccine K61 vaccine has preferably protection to tradition strain, but to variant
Protective rate only has 50%.
Table 3 composition A is to tradition strain and the protection situation of new variant
Table 3 result shows: composition A is no matter to tradition strain or novel variant strain all has preferable protective rate.
Experimental example 7
Pig farm, Jiangsu, child care pig 2500 bull, occur in that expiratory dyspnea, cough, have a running nose, nervous symptoms, diarrhoea, have
The clinical symptoms such as vomiting, the incidence of disease is about 35%, the death rate about 25%.Taking tonsillotome, brain tissue is through laboratory PCR
Detection, is diagnosed as porcine pseudorabies.Eliminate without using composition C spice administration in embodiment 1 after therapeutic value pig, per ton raise
Material adds 2kg, free choice feeding, uses 7 days continuously.3rd day state of an illness starts to take a turn for the better, and stops diffusion, and after medication 5 days, the state of an illness obtains
To stable, do not occur repeatedly after drug withdrawal.
As it has been described above, the preferred embodiments of the only present invention, it is illustrative rather than determinate, can be according to limited range
List several embodiments, therefore changing and modifications under without departing from present general inventive concept, the protection model of the present invention should be belonged to
Within enclosing.
Claims (9)
1. the composition of a resisting pstudorabies, it is characterised in that the composition of described resisting pstudorabies includes Chinese medicine composition and the Western medicine compound of 15-25 weight portion of 75-85 weight portion；
Described Chinese medicine composition is made up of the Chinese medicine material of following weight portion: Chinese bulbul 30-40 part, yncaria stem with hooks 30-40 part, root of kudzu vine 15-20 part, pine needle
15-20 part, capsule of weeping forsythia 20-30 part, honeysuckle 25-35 part, gynostemma pentaphylla 6-10 part；
Described Western medicine compound is the component mixing of volumes below part, makes after drying under reduced pressure: vitelline antibody for resisting pstudorabies 75-85 part, resisting pstudorabies transfer factor 15-25 part, inactivator 0.2-0.3 part.
Prepare resisting pstudorabies composition the most according to claim 1, it is characterized in that described vitelline antibody for resisting pstudorabies is adopted to prepare with the following method: use swine pseudorabies vaccine immune health laying hen, collect height and exempt from egg, take yolk, mix with water, obtain vitelline antibody for resisting pstudorabies；Described yolk is 1:2-4 with the mass ratio of water；Described resisting pstudorabies transfer factor is adopted and is prepared with the following method: takes the spleen that the pig of pseudorabies disease live-vaccine is crossed in immunity, adds the water of pig spleen volume 1.5-2.5, after homogenate, multigelation, regulates pH to 6.5-7.0, centrifugal, take supernatant, obtain resisting pstudorabies transfer factor.
The composition of resisting pstudorabies the most according to claim 1 or claim 2, it is characterised in that the composition of described resisting pstudorabies includes the Chinese medicine composition of 80 weight portions and the Western medicine compound of 20 parts.
The composition of resisting pstudorabies the most according to claim 3, it is characterised in that described Chinese medicine composition is made up of the Chinese medicine material of following weight portion: the Chinese bulbul
35 parts, yncaria stem with hooks 35 parts；The root of kudzu vine 18 parts, 18 parts of pine needle, the capsule of weeping forsythia 25 parts, honeysuckle 30 parts, gynostemma pentaphylla 8 parts；Described Western medicine compound be volumes below part component mixing after drying under reduced pressure make: vitelline antibody for resisting pstudorabies 80 parts, resisting pstudorabies transfer factor 20 parts, inactivator 0.2-0.3 part.
5. according to the composition of the described resisting pstudorabies of one of claim 1-4, it is characterised in that described inactivator is 37-40% formalin.
6. the preparation method of one of claim 1-5 described resisting pstudorabies composition, comprises the following steps:
(1) Chinese medicine composition is prepared: take the Chinese bulbul, yncaria stem with hooks, the root of kudzu vine, pine needle, the capsule of weeping forsythia, honeysuckle and gynostemma pentaphylla and be separately dried, grinding and sieving, mix, obtain described Chinese medicine composition；
(2) Western medicine compound is prepared；
(3) Western medicine compound is mixed with described Chinese medicine composition, obtain described resisting pstudorabies composition.
Prepare resisting pstudorabies composition the most according to claim 6, it is characterized in that described Western medicine compound is adopted to prepare with the following method: use swine pseudorabies vaccine immune health laying hen, collect height and exempt from egg, take yolk, mix with water, obtain vitelline antibody for resisting pstudorabies；Described yolk is 1:2-4 with the mass ratio of water；Take the spleen that the pig of pseudorabies disease live-vaccine is crossed in immunity, add the water of pig spleen volume 1.5-2.5 times, after homogenate, multigelation, regulate pH to 6.5-7.0, centrifugal, take supernatant, obtain resisting pstudorabies transfer factor；Vitelline antibody for resisting pstudorabies, resisting pstudorabies transfer factor and inactivator are mixed, filtration sterilization, after drying under reduced pressure, obtain described Western medicine compound.
8. contain the feed of one of claim 1-5 described resisting pstudorabies composition.
Feed the most according to claim 8, it is characterised in that in described feed, the mass percent of resisting pstudorabies composition is 0.15%-0.7%.
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| CN112868929A (en) * | 2021-01-13 | 2021-06-01 | 江西正邦动物保健品有限公司 | Pig additive containing saccharicterpenin and preparation method and application thereof |
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