CN105738617B - A kind of bladder chalone C latex intensified is than turbid detection kit and application thereof - Google Patents
A kind of bladder chalone C latex intensified is than turbid detection kit and application thereof Download PDFInfo
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- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
- G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
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Abstract
The present invention provides a kind of bladder chalone C latex intensified than turbid detection kit and application thereof, the kit, which includes reagent R1 and reagent R2, the reagent R1, includes buffer solution, and inorganic salts, surfactant, preservative, stabilizer and interference eliminate albumen；The reagent R2 includes buffer solution, inorganic salts, surfactant, preservative, stabilizer and polystyrene latex particles mixture；The polystyrene latex particles mixture is crosslinked with anti-bladder chalone C antibody；Kit of the present invention is based on Latex-enhanced immunoturbidimetric assay (PETIA), can be commonly used to the analysis of all kinds of automatic clinical chemistry analyzers, when use it is required minute it is short, specificity is good, and precision is high, and accuracy is good.
Description
Technical field
The present invention relates to immunologic assay analysis field, more particularly to a kind of bladder chalone C latex intensified is than turbid detection reagent
Box and application thereof.
Background technology
Since 1985, bladder chalone C (cystatin C) has been considered as detecting the good sign thing of renal function, due to
It has numerous superior from the influence of many physiological and pathological factors compared with other markers of glomerular filtration rate(GFR (GFR)
Property.Cystatin C are also played during series of physiological and pathological to be acted on, and has important clinical meaning.Bladder chalone C is one
Kind is the good marker for renal function diagnosis, it is thin to be present in internal all cores by the protein of CST3 gene codes
In born of the same parents.Bladder chalone C can freely filter mesangium, be the important symbol for indicating glomerulus rate filtering (GFR) function.
The development of immunoassay can date back 20th century mid-term, measure liquid phase immune agglutination at this time and cause optical density change
Optical technology developed.Occurs skeptophylaxis precipitation assay from the 1970s, i.e., immune transmission turbidimetric assay
With immune scattering turbidimetric assay, immunoturbidimetry, which measures, since then is gradually widely used in what protein content in clinical body fluid detected
Field.The immune turbid determination techniques of transmittance are widely used on automatic clinical chemistry analyzer, be immunized scattering turbidimetric assay then according to
Hold in the palm in special protein instrument, all realize high speed, sensitive and automation.
More commonly used at present is colloidal gold immunity chromatography, but the method sensitiveness is bad, bad, the Zhi Nengyong of repeatability
In qualitative detection, the effect of for clinical patient observation have limitation.Latex particle enhancing turbidimetry is relatively accurate at present, is stablized
Detection method.Turbidimetry (Turbidimetry)：Ray Of Light is molten by the suspension with fine particle and colloid
During liquid, remitted its fury that this solution be subject to two factors of light scattering and light absorbs is influenced that light can be made, in the degree and solution of decrease
The content of fine particle is directly proportional, by measuring transmitted intensity, derives the concentration of test substance in solution.To detect antigen
Exemplified by, in the case that amount of antibody is enough in reaction solution, determined antigen is more, and the antigen antibody complex of formation is also more, thoroughly
It is more to penetrate the remitted its fury of light, while is examined with other physical factors, such as antigen-antibody reaction time, light source power and wavelength, light
It is closely related to survey the distance between device and reaction cup etc..But there is also the bad linear dependence of precision is not strong for current technique
Problem.
The content of the invention
For the above problem of the prior art, the present invention provides a kind of bladder chalone C latex intensified than turbid detection reagent
Box and application thereof, the kit are based on Latex-enhanced immunoturbidimetric assay (PETIA), can be commonly used to all kinds of full-automatic raw
Change analyzer analysis, when use it is required minute it is short, specificity is good, and precision is high, and accuracy is good.
In order to achieve the above object, the present invention adopts the following technical scheme that：A kind of bladder chalone C latex intensified is than turbid detection examination
Agent box, including reagent R1 and reagent R2, the reagent R1 include buffer solution, inorganic salts, surfactant, preservative, stabilizer
And interference eliminates albumen；The reagent R2 includes buffer solution, inorganic salts, surfactant, preservative, stabilizer and polystyrene
Latex particle mixture；The polystyrene latex particles mixture is crosslinked with anti-bladder chalone C antibody；
The polystyrene latex particles mixture is major diameter polystyrene latex particles and minor diameter polystyrene colloidal
The mixture of newborn particle, the major diameter polystyrene latex particles average grain diameter is 400nm~600nm, and the minor diameter gathers
Styrene latex average grain diameter is 16nm~32nm, major diameter polystyrene latex particles:Minor diameter polystyrene latex particles
=(1-3):(7-9).
As it is further preferably, on the major diameter polystyrene latex particles and minor diameter polystyrene latex particles
All it is crosslinked with anti-bladder chalone C polyclonal antibody.Polystyrene latex particles are crosslinked the system of anti-bladder chalone C antibody in the reagent R2
Preparation Method is chemical crosslink technique, is specially the amino residue in anti-bladder chalone C antibody sequence and the polystyrene latex after activation
Particle surface carboxyl is chemically crosslinked by EDC.
As further preferably, each component and content of the reagent R1 are：
30~120mmol/L of buffer solution
80~300mmol/L of inorganic salts
3~10mmol/L of surfactant
Preservative 0.01%~1.0%
Interference eliminates albumen 0.5~1%
Stabilizer 1~10%.
Based on the quality of reagent R1, percentage is mass percent.
As further preferably, each component and content of the reagent R2 are：
30~120mmol/L of buffer solution
80~300mmol/L of inorganic salts
3~10mmol/L of surfactant
Preservative 0.01%~1.0%
Stabilizer 1~10%
Polystyrene latex particles mixture 0.1-1%.
Based on the quality of reagent R2, percentage is mass percent.
As further preferably, the buffer solution is 3- morpholine -2s-hydroxy-propanesulfonic acid (MOPSO), 2- (N- morpholines)
Ethyl sulfonic acid (MES), 4- hydroxyethyl piperazineethanesulfonic acids (HEPES), 3- [double (2- ethoxys) amino of N-N-] -2- hydroxy-propanesulfonic acids
(DIPSO), N- tri- (methylol) methylamino -2- hydroxy-propanesulfonic acids (TAPSO), N-2- hydroxyethyl piperazine-N'-3- propane sulfonic acid
(HEPPS) and the one or more in 3- (three (methylol) methyl) amino -1- propane sulfonic acid (TAPS)；The surfactant is
Fatty alcohol polyoxyethylene ether, octyl phenol polyoxyethylene ether, polyoxyethylene stearic acid ester, laurate polyoxyethylene ester, oleic acid polyoxy
One or more in vinyl acetate and lauryl amine polyoxyethylene ether；The stabilizer for protein, inorganic salts, metal chelating agent and
One or more in antioxidant；The interference eliminates the HBR blocking agents that albumen is Scantibodies companies；It is described inorganic
Salt is sodium chloride, the one or more in potassium chloride and magnesium sulfate；The preservative is Sodium azide, thimerosal and Proclin-
One or more in 300.
As further preferably, the stabilizer is chicken egg white and casein (casein).
As further preferably, each component and content of the reagent R1 are respectively：
As further preferably, each component and content of the reagent R2 are respectively：
As further preferably, the pH value of 2- (N- morpholines) ethyl sulfonic acids (MES) is 5.5-6.7.
As further preferably, the kit further includes the normative reference product of bladder chalone C reagent, the normative reference
Product recombinate the human serum of bladder chalone C albumen or the liquid of similar serum matrix for addition people.
As it is further preferably, the people recombinate bladder chalone C albumen by Pichia anomala expression, by affine ion exchange
Obtain after purification.
Preferably the normative reference product of the bladder chalone C reagent have 5 concentration as further, are respectively 0.5mg/L,
1mg/L, 2mg/L, 4mg/L, 8mg/L.
Purposes of the bladder chalone C latex intensified as described above than turbid detection kit bladder chalone C content in serum is detected.
In conclusion beneficial effects of the present invention are as follows：
(1), kit of the present invention measures bladder chalone C using Latex-enhanced immunoturbidimetric assay, is exaggerated detection signal,
Detection sensitivity is improved, shortens detection time.
(2), for the present invention using the emulsion reagent of mixing particle diameter, the latex particle ratio of wherein small particle is big, improves line
Property；Contain a certain proportion of large-sized latex particle at the same time, sensitivity is also higher.
(3), kit of the present invention has been specifically added the material for preventing interference, good in anti-interference performance.Meanwhile surfactant
Addition also improve precision.
(4), anti-bladder chalone C antibody is measured using automatic clinical chemistry analyzer, not only makes operation more easy, improved automatic
Change degree, and also a saving the financial cost of a small amount of sample detection experiment.Automatic clinical chemistry analyzer is multiple batches of to a small amount of
Sample is detected, and single batch is almost identical to the cost of a large amount of sample detections；Therefore, automatic clinical chemistry analyzer can fit
For the detection to a small amount of sample, and do not improve financial cost.
Embodiment
The present invention is by providing a kind of bladder chalone C latex intensified than turbid detection kit and application thereof, the kit base
In Latex-enhanced immunoturbidimetric assay (PETIA), all kinds of automatic clinical chemistry analyzers analyses can be commonly used to, when use is required
Minute it is short, specificity is good, and precision is high, and accuracy is good.
In order to better understand the above technical scheme, above-mentioned technical proposal is done in detail below in conjunction with specific embodiment
Thin explanation.
The embodiment of the present application bladder chalone C latex intensified is than turbid detection kit, including reagent R1 and reagent R2, the reagent
R1 includes buffer solution, and inorganic salts, surfactant, preservative, stabilizer and interference eliminate albumen；The reagent R2 includes buffering
Liquid, inorganic salts, surfactant, preservative, stabilizer and polystyrene latex particles mixture；
The polystyrene latex particles mixture is major diameter polystyrene latex particles and minor diameter polystyrene colloidal
The mixture of newborn particle, the major diameter polystyrene latex particles average grain diameter is 400nm~600nm, and the minor diameter gathers
Styrene latex average grain diameter is 16nm~32nm, major diameter polystyrene latex particles:Minor diameter polystyrene latex particles
=(1-3):(7-9).
The polystyrene latex particles mixture is crosslinked with anti-bladder chalone C antibody；The major diameter polystyrene latex
Anti- bladder chalone C polyclonal antibody is all crosslinked with particle and minor diameter polystyrene latex particles.The polystyrene latex
The preparation method that grain is crosslinked anti-bladder chalone C antibody is chemical crosslink technique, is specially the amino residue in anti-bladder chalone C antibody sequence
It is chemically crosslinked with the polystyrene latex particles surface carboxyl groups after activation by EDC.
The each component and content of the reagent R1 be respectively：
30~120mmol/L of buffer solution
80~300mmol/L of inorganic salts
3~10mmol/L of surfactant
Preservative 0.01%~1.0%
Interference eliminates albumen 0.5~1%
Stabilizer 1~10%.
Based on the quality of reagent R1, percentage is mass percent.
The each component and content of the reagent R2 be：
30~120mmol/L of buffer solution
80~300mmol/L of inorganic salts
3~10mmol/L of surfactant
Preservative 0.01%~1.0%
Stabilizer 1~10%
Polystyrene latex particles mixture 0.1-1%.
Based on the quality of reagent R2, percentage is mass percent.
The buffer solution is 3- morpholine -2s-hydroxy-propanesulfonic acid (MOPSO), 2- (N- morpholines) ethyl sulfonic acid (MES), 4- hydroxyl second
Base piperazine ethanesulfonic acid (HEPES), 3- [double (2- ethoxys) amino of N-N-] -2- hydroxy-propanesulfonic acids (DIPSO), N- tri- (methylol)
Methylamino -2- hydroxy-propanesulfonic acids (TAPSO), N-2- hydroxyethyl piperazine-N'-3- propane sulfonic acid (HEPPS) and 3- (three (methylol) first
Base) one or more in amino -1- propane sulfonic acid (TAPS)；The surfactant is fatty alcohol polyoxyethylene ether, octyl phenol
Polyoxyethylene ether, polyoxyethylene stearic acid ester, laurate polyoxyethylene ester, polyoxyethylene oleic acid ester and lauryl amine polyoxyethylene ether
In one or more；The stabilizer is the one or more in protein, inorganic salts, metal chelating agent and antioxidant；
The interference eliminates the HBR blocking agents that albumen is Scantibodies companies；The inorganic salts are sodium chloride, potassium chloride and sulfuric acid
One or more in magnesium；The preservative is the one or more in Sodium azide, thimerosal and Proclin-300.
The stabilizer is preferably chicken egg white and casein (casein).
Specifically, each component and concentration range of the reagent R1 are respectively：
Specifically, each component and concentration range of the reagent R2 are respectively：
The pH value of 2- (N- morpholines) ethyl sulfonic acids (MES) is 5.5-6.7.
The kit further includes the normative reference product of bladder chalone C reagent, and the normative reference product recombinate Guang for addition people
The liquid of the human serum of chalone C protein or similar serum matrix.
The people recombinates bladder chalone C albumen and is obtained by Pichia anomala expression, after affine ion-exchange purification.
The normative reference product of the bladder chalone C reagent have 5 concentration, respectively 0.5mg/L, 1mg/L, 2mg/L, 4mg/L,
8mg/L。
Embodiment 1
The each component and concentration range of the reagent R1 be：
The each component and concentration range of the reagent R2 be：
Reagent R1 is colourless transparent solution, and reagent R2 is homogeneous milky white solution, the reference calibrations product of bladder chalone C reagent
Also it is colourless transparent solution.
Embodiment 2
The each component and concentration range of the reagent R1 be：
The each component and concentration range of the reagent R2 be：
Reagent R1 is colourless transparent solution, and R2 is homogeneous milky white solution, and the reference calibrations product of bladder chalone C reagent are also
Colourless transparent solution.
1 R1 agent formulations of comparative example and embodiment 1 are identical, and R2 agent formulations and embodiment 1 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 400nm~
600nm, the minor diameter polystyrene latex average grain diameter are 16nm~32nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(7-10):(0-3).
2 R1 agent formulations of comparative example and embodiment 2 are identical, and R2 agent formulations and embodiment 2 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 400nm~
600nm, the minor diameter polystyrene latex average grain diameter are 10nm~12nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(1-3):(7-9).
3 R1 agent formulations of comparative example and embodiment 1 are identical, and R2 agent formulations and embodiment 1 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 400nm~
600nm, the minor diameter polystyrene latex average grain diameter are 40nm~100nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(1-3):(7-9).
4 R1 agent formulations of comparative example and embodiment 2 are identical, and R2 agent formulations and embodiment 2 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 200nm~
300nm, the minor diameter polystyrene latex average grain diameter are 16nm~32nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(1-3):(7-9).
5 R1 agent formulations of comparative example and embodiment 1 are identical, and R2 agent formulations and embodiment 1 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 700nm~
1000nm, the minor diameter polystyrene latex average grain diameter are 16nm~32nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(1-3):(7-9).
6 R1 agent formulations of comparative example and embodiment 2 are identical, and R2 agent formulations and embodiment 2 are different except latex diameter
Outside, remaining component is identical, and R2 latex diameters are respectively：The major diameter polystyrene latex particles average grain diameter for 400nm~
600nm, the minor diameter polystyrene latex average grain diameter are 16nm~32nm, major diameter polystyrene latex particles:It is small straight
Footpath polystyrene latex particles=(5-6):(4-5).
The correlation of the embodiment of the present application bladder chalone C kit, sensitivity, precision test:
Selected clinical sample is the patients serum that hospital collects, and the embodiment of the present application detection kit is detected in sample
The flow of bladder chalone C content is as follows：To 5uL samples to be detected, (calibration pipe makees sample with calibration object, and blank is using distilled water as sample
Product, calibration object are purchased from Landau company of Britain) in add the reagent R1 of 150uL and fully mix, constant temperature for 5 minutes in 37 DEG C, then to mixed
50uL reagent R2 are added in zoarium system, are mixed, 37 DEG C of constant temperature are after 1 minute, and blank tube zeroing, wavelength 540nm, measures each pipe extinction
A1 is spent, each pipe absorbance A 2 is measured after 4 minutes, calculates Δ A=A2-A1.Become according to multiple spot calibration object concentration and corresponding absorbance
Change value Δ A, determines working curve, sample absorbance change is corresponding on working curve using multiple spot gamma correction pattern
Concentration value is measured concentration.Following contrast agents are the bladder chalone C latex intensified ratio of double emulsion reagent methods of Ningbo Meikang
Turbid kit.
1 correlation is tested
It is full-automatic using Hitachi 7180 using the two kinds of reagents and contrast agents of Example 1 and Example 2 of the present invention respectively
Biochemical Analyzer is carried out at the same time measure to 50 parts of human serums (including normal and monstrosity) by each autoregressive parameter.Measured value is carried out
Linear regression, obtaining regression equation is：Y1=1.014x+1.8423, y2=1.024x+1.6553 related coefficient is：R21=
0.9965, related coefficient is：(y1 is 1 reagent of the embodiment of the present invention to R22=0.9947, and y2 is 2 reagent of the embodiment of the present invention, and x is
Contrast agents), the results showed that reagent of the present invention and contrast agents correlation are fine, and further relating to reagent of the present invention has well
Specificity and accuracy.In addition this reagent is applicable not only to 7180 automatic clinical chemistry analyzer of Hitachi, it may also be used for Hitachi, Bake
The full-automatic and semi-automatic biochemical analyzer of other series of graceful grade, design parameter can make the appropriate adjustments according to major parameter.Make respectively
With the two kinds of reagents and contrast agents of comparative example 1 of the present invention to comparative example 6 using 7180 full-automatic biochemical of Hitachi point
Analyzer is carried out at the same time measure to 50 parts of human serums (including normal and monstrosity) by each autoregressive parameter.Measured value is linearly returned
Return, obtaining regression equation is：Y1=1.113x+2.8526, y2=1.210x+2.0417, y3=1.035x+1.9591, y4=
1.073x+1.7660, y5=1.014x+1.9873, y6=1.009x+1.7860.R12=0.9878, R12=0.9274, R12
=0.9782, R12=0.9679, R12=0.9769, R12=0.9790, R12=0.9567,
2 sensitivity experiments
The standard deviation of 10-20 blank sample signal strength of the appraisal procedure of detection sensitivity, and 3-15 are a minimum non-
The standard deviation of zero sample signal strength, gained is calculated with statistical software EP Evaluator release 6.Experimental result is shown
Reagent of the present invention has good sensitivity, can comply fully with the improvement of U.S. clinical laboratory and amend legislation.Sensitivity is respectively：This
The bladder chalone C of invention is respectively 0.26mg/L and 0.32mg/L, better than contrast agents 0.37mg/L.
1 reagent detection sensitivity of table
3. precision test
Use the human serum sample of 2 kinds of different bladder chalone C contents, the withinrun precision of measure detection kit of the present invention.
The result shows that the withinrun precision of the detection kit of the embodiment of the present invention 1 and 2 is 1.47% and 2.86%；2.2% He
3.295%, the scheme provided according to NCCLS EP15-A files carries out the performance verification of precision.With three kinds of reagents of different manufacturers
Five kinds of serum specimens are measured daily 4 times, measure 5d altogether, calculate batch interior percentage variation of different manufacturers blood fat reagent detection
(CV), judge whether to be less than the data that producer provides.
Table 2
Same method measure comparative example 1 arrives the reagent of comparative example 6, as a result such as following table, it is seen that Data Representation is bright
Aobvious too late the embodiment of the present invention 1 and 2：
Table 3
4. the comparison of the interference free performance of two kinds of kits：
Four kinds of chaff interferents are added in Roche quality-control product (sign value 8.2mg/l) and form pooled serum, measurement result is as follows：
Total bilirubin determination reagent kit of the present invention and the contrast of existing kit measurement interference free performance：
Table 4
Technical solution in above-mentioned the embodiment of the present application, at least has the following technical effect that or advantage：
(1), kit of the present invention measures bladder chalone C using Latex-enhanced immunoturbidimetric assay, is exaggerated detection signal,
Detection sensitivity is improved, shortens detection time.
(2), for the present invention using the emulsion reagent of mixing particle diameter, the latex particle ratio of wherein small particle is big, improves line
Property；Contain a certain proportion of large-sized latex particle at the same time, sensitivity is also higher.
(3), kit of the present invention has been specifically added the material for preventing interference, good in anti-interference performance.Meanwhile surfactant
Addition also improve precision.
(4), anti-bladder chalone C antibody is measured using automatic clinical chemistry analyzer, not only makes operation more easy, improved automatic
Change degree, and also a saving the financial cost of a small amount of sample detection experiment.Automatic clinical chemistry analyzer is multiple batches of to a small amount of
Sample is detected, and single batch is almost identical to the cost of a large amount of sample detections；Therefore, automatic clinical chemistry analyzer can fit
For the detection to a small amount of sample, and do not improve financial cost.
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Property concept, then can make these embodiments other change and modification.So appended claims be intended to be construed to include it is excellent
Select embodiment and fall into all change and modification of the scope of the invention.Obviously, those skilled in the art can be to the present invention
Carry out various modification and variations without departing from the spirit and scope of the present invention.If in this way, these modifications and changes of the present invention
Belong within the scope of the claims in the present invention and its equivalent technologies, then the present invention is also intended to exist comprising these modification and variations
It is interior.
Claims (3)
1. a kind of bladder chalone C latex intensified is than turbid detection kit, it is characterised in that：Including reagent R1 and reagent R2, the examination
The each component and content of agent R1 be respectively：
The each component and content of the reagent R2 be respectively：
The polystyrene latex particles mixture is crosslinked with anti-bladder chalone C antibody；
The polystyrene latex particles mixture is major diameter polystyrene latex particles and minor diameter polystyrene latex
The mixture of grain, the major diameter polystyrene latex particles average grain diameter are 400nm~600nm, the minor diameter polyphenyl second
Alkene latex average grain diameter is 16nm~32nm, and major diameter polystyrene latex particles are matched somebody with somebody with minor diameter polystyrene latex particles
Than for (1-3):(7-9).
2. bladder chalone C latex intensified according to claim 1 is than turbid detection kit, it is characterised in that：The kit
The normative reference product of bladder chalone C reagent are further included, the normative reference product recombinate the human serum of bladder chalone C albumen for addition people.
3. bladder chalone C latex intensified according to claim 2 is than turbid detection kit, it is characterised in that：The bladder chalone C
The normative reference product of reagent have 5 concentration, are respectively 0.5mg/L, 1mg/L, 2mg/L, 4mg/L and 8mg/L.
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| CN101377492B (en) * | 2007-08-29 | 2014-04-23 | 北京九强生物技术股份有限公司 | Bladder chalone C determining reagent kit |
| US20100240150A1 (en) * | 2007-11-21 | 2010-09-23 | Arkray, Inc. | Measuring reagent and turbidimetric immunoassay and sample analysis tool using the same |
| CN101498729A (en) * | 2009-03-09 | 2009-08-05 | 浙江理工大学 | Immunochromatography test paper for fast diagnosis of human granulocytic anaplasmosis and its preparation |
| EP2799874A4 (en) * | 2011-12-28 | 2015-08-12 | Sekisui Medical Co Ltd | Latex particles for measuring particle agglomeration |
| CN102590524B (en) * | 2011-12-30 | 2016-01-06 | 北京九强生物技术股份有限公司 | Neutrophil gelatinase-associated lipocalin detection kit |
| CN102590497B (en) * | 2012-01-13 | 2014-01-01 | 宁波美康生物科技股份有限公司 | Cysteine protease inhibitor C test kit |
| CN102636647B (en) * | 2012-03-31 | 2014-05-21 | 戴国华 | Ketamine-collaurum test paper for detection of saliva |
| CN102636653A (en) * | 2012-04-19 | 2012-08-15 | 上海蓝怡科技有限公司 | Compounded latex particle-enveloped cystatin C detection kit |
| CN102879569A (en) * | 2012-09-18 | 2013-01-16 | 苏州照康生物技术有限公司 | Immunoturbidimetry kit for detecting cysteine proteinase inhibitor C and preparation method thereof |
| CN103472235A (en) * | 2013-08-26 | 2013-12-25 | 河北省科学院生物研究所 | Long-acting protein solution stabilizing agent |
| CN103823070A (en) * | 2014-03-21 | 2014-05-28 | 北京强申医学科技有限公司 | Cystatin C determination kit with high sensitivity |
| CN104360081B (en) * | 2014-12-05 | 2016-03-09 | 重庆中元生物技术有限公司 | Cystatin C detection kit of a kind of improvement and preparation method thereof |
| CN105158476A (en) * | 2015-06-03 | 2015-12-16 | 南京闻智生物科技有限公司 | Full-scale range C-reactive protein latex-enhanced immunoturbidimetry detection kit |
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