CN105717243A - Interface for two-dimensional liquid chromatography - Google Patents
Interface for two-dimensional liquid chromatography Download PDFInfo
- Publication number
- CN105717243A CN105717243A CN201410723395.8A CN201410723395A CN105717243A CN 105717243 A CN105717243 A CN 105717243A CN 201410723395 A CN201410723395 A CN 201410723395A CN 105717243 A CN105717243 A CN 105717243A
- Authority
- CN
- China
- Prior art keywords
- liquid chromatography
- way valve
- dimensional
- interface
- hollow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to an interface for two-dimensional liquid chromatography, specifically to an interface based on a hollow fibrous membrane and used for two-dimensional coupled liquid chromatography. The interface comprises a hollow fibrous membrane assembly, a ten-way valve, two fraction collecting quantitative loops, a heater, a vacuum pump and a damper tube. When a first dimensional chromatographic distillate passes through the hollow fibrous membrane assembly in the interface, solvents in the distillate are continuously removed, and a sample in the distillate is retained and passes through the hollow fibrous membrane assembly without loss. The quantitative loops in the ten-way valve of the interface collect and cut the concentrated fraction and transfer the fraction to a second dimensional chromatographic column for subsequent separation. The interface provided by the invention reduces the sample introduction volume of second dimensional liquid chromatography, increases the concentration of the sample, prevents reduction in separation efficiency of a two-dimensional chromatographic column due to large-volume sample introduction and improves separation efficiency and detection sensitivity. The interface is applicable to coupled liquid chromatography of various separation modes and can greatly improve the orthogonal degree, peak capacity and detection limits of two-dimensional liquid chromatography and broaden the application scope of two-dimensional liquid chromatography.
Description
Technical field
The present invention relates to liquid chromatograph instrument analysis field, relate in particular to a kind of two-dimensional liquid chromatography interface.This interface can remove the most of solvent in the first dimension liquid chromatograph distillation continuously, reduces the sampling volume of two-dimensional liquid chromatography, adds sample concentration, improves detection sensitivity, is suitable for various clastotype liquid chromatograph couplings.
Background technology
The impact of the first dimension flowing relative sample later separation and detection is always up the root problem of restriction two-dimensional liquid chromatography development and practical application: when (1) bidimensional mobile phase does not dissolve each other or viscosity differences is bigger, two dimensional separation is unstable；(2) relative second dimension chromatographic column first ties up mobile phase when being strong solvent, the second dimension sample introduction widening diffusion；(3) sample separates through the first dimension, is tieed up mobile phase by first and dilutes, spread, it is necessary to " again focusing on "；(4) volume cutting fraction generally far surpasses the maximum sampling volume of the second dimension.During due to the existence of the problems referred to above, design and structure two-dimensional liquid chromatography system, its clastotype and separation condition are affected by great restriction, and intercept, peak capacity and theoretical value difference that system is actual are bigger, it is impossible to give full play to the advantage of two dimensional separation.Meanwhile, the detection limit of system has been also affected by strong influence.
One of substitutive characteristics of Two way chromatograms is that bidimensional separation does not independently interfere with each other, and the problem that affects how effectively solving the first dimension flowing relative sample later separation and detection is always up the emphasis of two-dimensional liquid chromatography research.In recent years, along with the development of high performance liquid chromatograph device hardware, two-dimensional liquid chromatography technology also obtain significant progress, applies increasingly extensive, but the problems referred to above are not solved, and still continues to use traditional certain methods.As being widely adopted at present first ties up fine inner diameter long column, is easily separated at low flow rates, and second ties up the method that thick post runs under high flow rate.This method alleviates, by reducing the amount of the first dimension mobile phase, the series of problems that in cutting fraction, first dimension mobile phase entrance the second dimension is brought.Dugo et al. establishes NPLC the method based on the method and sets up analysis classes carotene, capillary column is used in the first dimension, significantly reduce the volume of cutting fraction, and a small amount of positive mobile phase is when entering the second dimension chromatographic column, by a large amount of Flow Injection Chemiluminescence Method phase dilutions, reverse phase separation can can't be caused appreciable impact.But the problem of the dilution of sample and " focusing " is not still solved, whole two-dimentional system volume containing the sample reduces simultaneously, is unfavorable for the separation detection of low content component.
Thoroughly solving the problems referred to above the most direct the simplest thinking is adopt " cold-trap " design in a similar two-dimensional gas chromatography interface in the interface, the first dimension mobile phase in cutting fraction is removed, it is achieved the concentration of sample and " focusing " before cutting fraction enters the second dimension chromatographic column.Realize this thinking to need to meet two primary conditions: the transfer that (1) sample is pollution-free, lossless；(2) time removing solvent must be equal with the fraction collection time, to meet the practical application request of comprehensive two dimensional gas chromatography.The Interface design embodying this thinking has trapping and part evaporation.
Trapping is the quantitative loop using trapping column to replace in interface, under first dimension elution requirement, sample adsorbs at trapping column stigma, and tie up sample under elution requirement second and be eluted into the second dimension chromatographic column completely and carry out later separation, it is achieved that concentration and " focusing " to sample.The filler of trapping column and the selection of bidimensional mobile phase are had certain restriction by this method, and bidimensional separation can not be truly realized separate, and trapping needs to obtain good effect under given conditions.
Owing to mobile phase boiling point is much smaller than sample, the first dimension mobile phase in evaporative removal cutting fraction therefore can be passed through, it is achieved the concentration of sample and " focusing ".Nineteen eighty-two, Wise et al. establishes the polycyclic arene compound of NPLC/RPLC systematic analysis oil product kind, introduces nitrogen in the interface and purges cutting fraction, the positive phase solvent in evaporative removal fraction.But its evaporation rate is slower, it is impossible to meet the requirement that the first dimension is continuously separated and complicated operation, it is impossible to automatization.Guan Yafeng researcher seminar of the Dalian Chemistry and Physics Institute has developed a kind of vacuum auxiliary-solvent evaporation interface, it is possible to continuous evaporation removes flow up to 1mL/min organic solvent, nonvolatile element retained concentration in interface quantitative loop in sample.Vacuum auxiliary-solvent evaporation interface solves the problem that the first dimension uses solvent during high volatile organic solvent to remove online, but this interface can not realize the online removal of the relatively low intensive polar solvent of volatility.
The development of this style interface to solve following two key issue: (1) allows for adapting to various the first different dimension solvent.Desirable two-dimensional liquid chromatography, the clastotype of bidimensional is different and separate does not interfere with each other, it is possible to each independent optimization separation condition.And liquid chromatograph has multiple clastotype, it is possible to use mobile phase kind a lot, particularly when the first dimension makes gradient elution, interface must also adapt to the change of mixed flow phase concentration and pressure.(2) removal rate of solvent.The flow of conventional liquid phase chromatograph is generally at 1mL/min, and interface at least needs to reduce more than first dimension one order of magnitude of flow, and the removal of solvents time must with the fraction collection time equal demand that could meet Comprehensive two-dimensional LC practical application.Traditional interface trapping and part evaporation interface are restricted in principle, it is impossible to widely use in two-dimensional liquid chromatography as a kind of general method.
Summary of the invention
It is an object of the invention to provide a kind of combined with two-dimensional liquid chromatography interface based on hollow-fibre membrane, when first ties up the hollow fiber film assembly that chromatograph distillation passes through in interface, the solvent in distillation is continuously removed, and the sample in distillation is then lossless to be passed through.Quantitative loop in interface ten-way valve collects the fraction after cutting concentration, and is transferred to the second dimension chromatographic column and carries out later separation.
For achieving the above object, the technical solution used in the present invention is:
A kind of two-dimensional liquid chromatography interface, it is characterised in that: it is made up of hollow fiber film assembly, ten-way valve, fraction collection quantitative loop 1, fraction collection quantitative loop 2, heater, vacuum pump and damper tube；The two ends of described fraction collection quantitative loop 1 be connected to ten-way valve 2., 7. position, the two ends of fraction collection quantitative loop 2 be connected to ten-way valve 5., 10. on position；9. the position of ten-way valve is tieed up liquid chromatography pump with second and is connected, and the 6. position of ten-way valve is tieed up liquid chromatograph column inlet with second and is connected, and the 4. position of ten-way valve is connected with damper tube；Described hollow fiber film assembly is made up of hollow-fibre membrane and vacuum chamber, and hollow-fibre membrane is placed in the inside of vacuum chamber, makes to be formed between hollow-fibre membrane outer wall and the inwall of vacuum chamber the cavity of sealing；The arrival end of hollow-fibre membrane and the first dimension liquid chromatograph column outlet are connected, and the port of export of hollow-fibre membrane is connected with ten-way valve 1. position；The sidewall of vacuum chamber there is an interface be connected with vacuum pump；Hollow fiber film assembly is heated by heater.
Described ten-way valve can be replaced by one or two or more kinds combination in more than 2 eight logical valve, six-way valve, cross valves.
Described ten-way valve, fraction collection quantitative loop 1 and fraction collection quantitative loop 2 can replace by a six-way valve and a fraction collection quantitative loop, for heartcut formula two-dimensional liquid chromatography interface.
Described heater adopts the mode of Infrared Heating to be hollow fiber film assembly heating, to maintain hollow fiber film assembly constant temperature.
Pressure in described hollow-fibre membrane is adjusted by the length and internal diameter changing damper tube.
The threshold value that retains of described hollow-fibre membrane ties up the solvent molecule size that mobile phase uses more than first, less than sample molecule size.
Ten-way valve (200) 3., 8. position be connected.
When adopting such scheme, sample is after first ties up chromatographic column separation, during by hollow fiber film assembly, due to first tie up that the solvent molecule that mobile phase uses is smaller in size than hollow-fibre membrane retain threshold value, under the promotion of film pressure at both sides difference, solvent is rejected to outside through hollow-fibre membrane and is removed by evaporation, and the molecular dimension of sample is retained more than the threshold value that retains of film, and sample is concentrated.Vacuum pump is the vacuum needed for hollow fiber film assembly offer, makes the solvent passing through hollow-fibre membrane to evaporate in real time, improves the solvent speed through hollow-fibre membrane；Meanwhile, heater is hollow fiber film assembly heating, it is provided that solvent evaporation institute calorific requirement, and makes hollow fiber film assembly maintain higher temperature, improves the solvent speed through hollow-fibre membrane.By adjusting the damper tube being connected on ten-way valve, change the pressure in hollow-fibre membrane, it is possible to regulate the solvent speed through hollow-fibre membrane.It is connected on ten-way valve two quantitative loop and collects the fraction after cutting through hollow fiber film assembly in turn, tieed up mobile phase by second and promote, be transferred to Two way chromatograms and carry out later separation detection.
Compared with prior art, present invention have the advantage that
1. membrane separation technique is introduced in two-dimensional liquid chromatography interface, the most of solvent in the first dimension liquid chromatograph distillation can be removed continuously in real time, reduce the sampling volume of two-dimensional liquid chromatography, increase sample concentration, avoid reducing because of large volume sample injection the separation efficiency of Two way chromatograms post, improve separation efficiency and detection sensitivity.
2. the removal rate of solvent is fast, and when the first dimension flow velocity reaches 1mL/min, interface also can remove the solvent of more than 90% continuously in real time, and the first dimension chromatograph can use conventional liquid phase chromatographic column.By adjusting the damper tube on ten-way valve, it is possible to regulate the removal rate of solvent, simple and convenient.
3. applied range, not by the restriction of solvent species and the first dimension gradient elution.Interface can apply to various clastotype liquid chromatograph coupling, and bidimensional is separate not to be interfere with each other, it is possible to each independent optimization separation condition, greatly improves the intercept of two-dimensional liquid chromatography, peak capacity and detection limit.
Accompanying drawing explanation
Fig. 1 is the two-dimensional liquid chromatography interface diagram of the present invention.In figure: 100-hollow fiber film assembly；101-hollow-fibre membrane；102-vacuum chamber；200-ten-way valve；301-fraction collection quantitative loop 1；302-fraction collection quantitative loop 2；400-heater；500-vacuum pump；600-damper tube.
Fig. 2 is the Comprehensive two-dimensional LC system connection diagram of the two-dimensional liquid chromatography interface adopting the present invention.In figure: 1-first ties up chromatographic column；2-second ties up chromatographic column；3-liquid chromatography pump.
Fig. 3 is the heartcut pattern two-dimensional liquid chromatography system connection diagram of the two-dimensional liquid chromatography interface adopting the present invention.In figure: 100-hollow fiber film assembly；101-hollow-fibre membrane；102-vacuum chamber；201-six-way valve；303-fraction collection quantitative loop 3；400-heater；500-vacuum pump；600-damper tube.1-first ties up chromatographic column；2-second ties up chromatographic column；3-liquid chromatography pump；4-one-dimensional detector.
Fig. 4 is the separation spectrogram that embodiment 1 adopts Comprehensive two-dimensional LC analytical standard protein solution.
Fig. 5 is that embodiment 2 adopts two-dimensional liquid chromatography to analyze the separation spectrogram of polystyrene solution.Wherein (a) is the first dimension separation spectrogram, and (b), (c), (d) respectively the first dimension separates the second dimension of corresponding cutting of components in spectrogram and separates spectrogram.
Detailed description of the invention
A kind of two-dimensional liquid chromatography interface based on hollow-fibre membrane, as shown in Figure 1；Adopt the two-dimensional liquid chromatography system of this interface as shown in Figure 2.Sample is tieed up chromatogram pump by first and is promoted, and ties up through first and flows out after post after chromatographic column 1 separates, by hollow fiber film assembly 100.When first dimension distillation is by hollow fiber film assembly 100, owing to the most of solvent in distillation is removed, the flow of distillation is greatly reduced, and the sample in distillation is then retained, and lossless passes through.Distillation is by, after hollow fiber film assembly 100, being collected storage by the fraction collection quantitative loop 301 on ten-way valve 200.After fraction collection quantitative loop 301 gathers fraction, rotating ten-way valve 200, the fraction collected in fraction collection quantitative loop 301 is transferred in the second dimension chromatographic column 2 and is carried out two dimensional separation by the second dimension chromatogram pump 3.Meanwhile, fraction collection quantitative loop 302 is collected and is stored the first dimension distillation.The time of fraction collection is identical with the time that Two way chromatograms completes first separation, depends on that the volume of fraction collection quantitative loop 301,302 and distillation are by the flow velocity after hollow fiber film assembly 100.After fraction collection quantitative loop 302 gathers fraction, rotate ten-way valve 200, the fraction collected in fraction collection quantitative loop 302 is transferred to and is carried out two dimensional separation in the second dimension chromatographic column 2 by the second dimension chromatogram pump 3, and fraction collection quantitative loop 301 is collected and stored the first dimension distillation simultaneously.So move in circles, until completing the Comprehensive two-dimensional LC separation detection of sample.
Embodiment 1
Experimental provision is as shown in Figure 2.First dimension chromatographic column is fill the WatersDEAE8HR Weak anion-exchange chromatography post 100mm × 4.6mm in 8 μm of particle diameter apertures.Second dimension chromatographic column is fill the HypersilBDSC18 reversed phase chromatographic column 35mm × 4.6mm of 5 μm of particle diameters.Hollow-fibre membrane internal diameter 0.4mm, the length 10cm used in hollow fiber film assembly, molecular cut off is 2000.Assembly vacuum cavity pressure 200Pa, assembly temperature is 50 DEG C.Fraction collection quantitative loop volume is 150 μ L, and the first dimension mobile phase is after hollow-fibre membrane, and flow-reduction is 67 μ L/min, and the second dimension chromatography column feed materials frequency and interface valve switching frequency are 1 time/2min.Sample is standard protein solution, sampling volume 10 μ L.Detector is Variable wavelength UV detector, detects wavelength 210nm.Analysis condition: the first dimension mobile phase mobile phase pH=7.5, A is 20mmol/LTris-HC1, B is A+0.5mol/LNaC1；Flow velocity 1.0mL/min, gradient elution program is 0～8min, 0～60%B.Second dimension mobile phase A is the water containing 0.1% trifluoroacetic acid (TFA), and B is the acetonitrile containing 0.1%TFA；Flow velocity 3.0mL/min, gradient elution program is 0～1.5min, 20%～80%B, 1.5～2.0min, 80%～20%B.Full two dimensional separation spectrogram is shown in Fig. 4, wherein 1-ribonuclease, 2-cytochrome C, 3-lysozyme (Lys), 4-egg albumin, 5-bovine serum albumin.
Embodiment 2
Experimental provision is as shown in Figure 3.First dimension chromatographic column is fill the HypersilBDSC18 reversed phase chromatographic column 250mm × 4.6mm of 5 μm of particle diameters.Second dimension chromatographic column is fill the Zr-CarbonC18 chromatographic column 50mm × 4.6mm of 3 μm of particle diameters.The doughnut retaining molecular weight used in hollow fiber film assembly is 1000, internal diameter 0.2mm, length 15cm.Assembly vacuum cavity pressure 200Pa, assembly temperature is 50 DEG C.Fraction collection quantitative loop volume is 120 μ L, and the first dimension mobile phase is after hollow-fibre membrane, and flow-reduction is 50 μ L/min.Sample is polystyrene solution, sampling volume 5 μ L.Detector is Variable wavelength UV detector, detects wavelength 262nm.Analysis condition: the first dimension mobile phase mobile phase methanol, flow velocity 1.0mL/min.Second dimension mobile phase acetonitrile, flow velocity 1.0mL/min, isocratic elution.Two dimensional separation spectrogram is shown in Fig. 5.
Claims (7)
1. a two-dimensional liquid chromatography interface, it is characterised in that: it is made up of hollow fiber film assembly (100), ten-way valve (200), fraction collection quantitative loop 1 (301), fraction collection quantitative loop 2 (302), heater (400), vacuum pump (500) and damper tube (600)；The two ends of described fraction collection quantitative loop 1 (301) be connected to ten-way valve (200) 2., 7. position, the two ends of fraction collection quantitative loop 2 (302) be connected to ten-way valve (200) 5., 10. on position；9. the position of ten-way valve (200) is tieed up liquid chromatography pump with second and is connected, and the 6. position of ten-way valve (200) is tieed up liquid chromatograph column inlet with second and is connected, and the 4. position of ten-way valve (200) is connected with damper tube (600)；Described hollow fiber film assembly (100) is made up of hollow-fibre membrane (101) and vacuum chamber (102), hollow-fibre membrane (101) is placed in the inside of vacuum chamber (102), makes to be formed between the inwall of hollow-fibre membrane (101) outer wall and vacuum chamber (102) cavity of sealing；The arrival end of hollow-fibre membrane (101) and the first dimension liquid chromatograph column outlet are connected, and the port of export of hollow-fibre membrane (101) is connected with ten-way valve (200) 1. position；The sidewall of vacuum chamber (102) there is an interface be connected with vacuum pump (500)；Hollow fiber film assembly (100) is heated by heater (400).
2. two-dimensional liquid chromatography interface according to claim 1, it is characterised in that: described ten-way valve (200) is replaced by one or two or more kinds combination in more than 2 eight logical valve, six-way valve, cross valves.
3. two-dimensional liquid chromatography interface according to claim 1, it is characterized in that: a six-way valve of described ten-way valve (200), fraction collection quantitative loop 1 (301) and fraction collection quantitative loop 2 (302) and a fraction collection quantitative loop replace, for heartcut formula two-dimensional liquid chromatography interface.
4. two-dimensional liquid chromatography interface according to claim 1, it is characterized in that: described heater (400) adopts the mode of Infrared Heating to be hollow fiber film assembly (100) heating, to maintain hollow fiber film assembly (100) constant temperature.
5. two-dimensional liquid chromatography interface according to claim 1, it is characterised in that: the pressure in described hollow-fibre membrane (101) is adjusted by the length and internal diameter changing damper tube (600).
6. two-dimensional liquid chromatography interface according to claim 1, it is characterised in that: the threshold value that retains of described hollow-fibre membrane (101) ties up the solvent molecule size that mobile phase uses more than first, less than sample molecule size.
7. two-dimensional liquid chromatography interface according to claim 1, it is characterised in that: ten-way valve (200) 3., 8. position be connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723395.8A CN105717243A (en) | 2014-12-03 | 2014-12-03 | Interface for two-dimensional liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410723395.8A CN105717243A (en) | 2014-12-03 | 2014-12-03 | Interface for two-dimensional liquid chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105717243A true CN105717243A (en) | 2016-06-29 |
Family
ID=56146596
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410723395.8A Pending CN105717243A (en) | 2014-12-03 | 2014-12-03 | Interface for two-dimensional liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105717243A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106443043A (en) * | 2016-11-15 | 2017-02-22 | 成都市食品药品检验研究院 | Continuous hydride generation atomic fluorescence sample injection system |
| US20200209124A1 (en) * | 2017-05-19 | 2020-07-02 | University Of Tasmania | Device and method for interfacing two separation techniques |
| CN112946292A (en) * | 2019-12-10 | 2021-06-11 | 中国科学院大连化学物理研究所 | Method for quantitatively detecting target protein in single cell |
| CN115326967A (en) * | 2022-08-16 | 2022-11-11 | 成都蓝湖科技有限公司 | Sample pretreatment system for on-line chromatographic analyzer for measuring natural gas components |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448691A (en) * | 1980-09-02 | 1984-05-15 | The Dow Chemical Company | Liquid chromatographic method and apparatus with hollow fiber device for post-column derivatization |
| JP2002214216A (en) * | 2001-01-12 | 2002-07-31 | Mitsubishi Electric Corp | High-speed analyzing instrument for monochlorobenzene and high-speed analyzing method using the same |
| JP2003262625A (en) * | 2002-03-07 | 2003-09-19 | Mitsubishi Electric Corp | System and method for monitoring air-borne organic matter |
| CN1477391A (en) * | 2002-08-21 | 2004-02-25 | 中国科学院大连化学物理研究所 | Method for sedparating bialogical macromolecule by using two-dimensinal or multi-dimensinal capillary electrophoresis and its used interface |
| CN101545889A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Vacuum auxiliary-solvent evaporation interface combined with two-dimensional liquid chromatography |
| CN203772816U (en) * | 2014-03-07 | 2014-08-13 | 天津出入境检验检疫局动植物与食品检测中心 | Two-dimensional liquid chromatography-mass spectrometer for detecting strong polar compounds |
-
2014
- 2014-12-03 CN CN201410723395.8A patent/CN105717243A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4448691A (en) * | 1980-09-02 | 1984-05-15 | The Dow Chemical Company | Liquid chromatographic method and apparatus with hollow fiber device for post-column derivatization |
| JP2002214216A (en) * | 2001-01-12 | 2002-07-31 | Mitsubishi Electric Corp | High-speed analyzing instrument for monochlorobenzene and high-speed analyzing method using the same |
| JP2003262625A (en) * | 2002-03-07 | 2003-09-19 | Mitsubishi Electric Corp | System and method for monitoring air-borne organic matter |
| CN1477391A (en) * | 2002-08-21 | 2004-02-25 | 中国科学院大连化学物理研究所 | Method for sedparating bialogical macromolecule by using two-dimensinal or multi-dimensinal capillary electrophoresis and its used interface |
| CN101545889A (en) * | 2008-03-28 | 2009-09-30 | 中国科学院大连化学物理研究所 | Vacuum auxiliary-solvent evaporation interface combined with two-dimensional liquid chromatography |
| CN203772816U (en) * | 2014-03-07 | 2014-08-13 | 天津出入境检验检疫局动植物与食品检测中心 | Two-dimensional liquid chromatography-mass spectrometer for detecting strong polar compounds |
Non-Patent Citations (4)
| Title |
|---|
| C. TRAGAS ET AL.: "On-line coupling of high performance gel filtration chromatography with imaged capillary isoelectric ocusing using a membrane interface", 《ELECTROPHORESIS》 * |
| HUIMING YUAN ET AL.: "Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion", 《ANALYTICAL CHEMISTRY》 * |
| XIAOQIANG QIAO ET AL.: "High sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization", 《JOURNAL OF CHROMATOGRAPHY B》 * |
| 田宏哲 等: "真空溶剂蒸发全二维液相色谱接口及其应用", 《分析化学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106443043A (en) * | 2016-11-15 | 2017-02-22 | 成都市食品药品检验研究院 | Continuous hydride generation atomic fluorescence sample injection system |
| CN106443043B (en) * | 2016-11-15 | 2019-02-12 | 成都市食品药品检验研究院 | Atomic fluorescence sampling system occurs for continuously hydrogenating object |
| US20200209124A1 (en) * | 2017-05-19 | 2020-07-02 | University Of Tasmania | Device and method for interfacing two separation techniques |
| CN112946292A (en) * | 2019-12-10 | 2021-06-11 | 中国科学院大连化学物理研究所 | Method for quantitatively detecting target protein in single cell |
| CN112946292B (en) * | 2019-12-10 | 2022-06-21 | 中国科学院大连化学物理研究所 | Method for quantitatively detecting target protein in single cell |
| CN115326967A (en) * | 2022-08-16 | 2022-11-11 | 成都蓝湖科技有限公司 | Sample pretreatment system for on-line chromatographic analyzer for measuring natural gas components |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11340199B2 (en) | Systems and methods for two-dimensional chromatography | |
| CN104713973B (en) | There is two-dimentional preparative hplc instrument system and the application thereof of on-line preconcentration function | |
| JP4009671B2 (en) | Discontinuous countercurrent chromatography method and apparatus | |
| US8968561B2 (en) | Preparative separation/purification system | |
| CN105717243A (en) | Interface for two-dimensional liquid chromatography | |
| CN108562678B (en) | Three-dimensional liquid chromatographic separation system based on full online detection of same detector | |
| Malerod et al. | Recent advances in on-line multidimensional liquid chromatography | |
| JP2014514541A (en) | Valves and splitting systems for multidimensional liquid analysis | |
| Ding et al. | A vacuum assisted dynamic evaporation interface for two-dimensional normal phase/reverse phase liquid chromatography | |
| Li et al. | On-line comprehensive two-dimensional normal-phase liquid chromatography× reversed-phase liquid chromatography for preparative isolation of toad venom | |
| CN105319310A (en) | A method and system for monitoring biomolecule separations by mass spectrometry | |
| US20170010243A1 (en) | Multiple column chromatographic system and methods of use | |
| CN107589190A (en) | The double SPE high performance liquid chromatography on-line coupling equipment of large volume sample injection | |
| CN101545889A (en) | Vacuum auxiliary-solvent evaporation interface combined with two-dimensional liquid chromatography | |
| JP6260719B2 (en) | Liquid chromatograph | |
| CN203772816U (en) | Two-dimensional liquid chromatography-mass spectrometer for detecting strong polar compounds | |
| CN106075954A (en) | Type two-dimensional liquid chromatography and application thereof is arrheaed with multiple-way valve as switching device | |
| CN108490107A (en) | A kind of liquid chromatograph and working method for complex sample multi-cycle separation | |
| Stoll | Mixing and Mixers in liquid chromatography—Why, When, and How Much? Part I, The Pump | |
| CN205786515U (en) | A kind of arrhea type two-dimensional liquid chromatography with two multiple-way valves as switching device | |
| CN107764920A (en) | Comprehensive two dimensional gas chromatography instrument and its optimization modulator approach | |
| CN109932464B (en) | Multidimensional chromatography system and multidimensional chromatography separation method | |
| Liu et al. | Two‐dimensional LC–MS analysis of components in Swertia franchetiana Smith | |
| CN211505348U (en) | Multidimensional liquid chromatography separation device | |
| CN215415250U (en) | Production type circulating multidimensional liquid chromatography separation system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160629 |
|
| RJ01 | Rejection of invention patent application after publication |