CN105707057A - Method for unfreezing gamete or embryo on frozen carrier and vessel special for vitrification unfreezing - Google Patents
Method for unfreezing gamete or embryo on frozen carrier and vessel special for vitrification unfreezing Download PDFInfo
- Publication number
- CN105707057A CN105707057A CN201610242377.7A CN201610242377A CN105707057A CN 105707057 A CN105707057 A CN 105707057A CN 201610242377 A CN201610242377 A CN 201610242377A CN 105707057 A CN105707057 A CN 105707057A
- Authority
- CN
- China
- Prior art keywords
- gamete
- embryo
- defrosting
- fluid apertures
- unfreezing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a method for unfreezing a gamete or an embryo on a frozen carrier.The method sequentially includes the following steps that a vessel special for vitrification unfreezing is prepared, a vessel disc of the vessel special for vitrification unfreezing is provided with an unfreezing liquid hole with the upper portion being open, the left side wall of the unfreezing liquid hole is an inclined plane, and the bottom of the unfreezing liquid hole is a flat bottom; unfreezing liquid is added into the unfreezing liquid hole; a frozen carrying pole is adopted to take the gamete or the embryo out of liquid nitrogen, the gamete or the embryo is located on a blade of the frozen carrying pole, and the blade is made to downwards move in an inclined mode along the left side wall of the unfreezing liquid hole till the blade is soaked in the unfreezing liquid; a frozen carrying pole is utilized to observe that the gamete or the embryo falls.The method has the advantages that the gamete or the embryo on the frozen carrier can be unfrozen rapidly and accurately, so that developmental potentiality and pregnancy outcome of the gamete or the embryo are improved.The invention further discloses the vessel special for vitrification unfreezing.
Description
Technical field
The present invention relates to field of medical technology, especially relate to a kind of gamete or embryo thawing method on freezing carrier.The invention still further relates to gamete on this freezing carrier or the vitrification used in embryo thawing method is thawed special ware.
Background technology
At present, when gamete on freezing carrier or embryo are thawed, thawing liquid required in culture dish is more, and need to adjust in time field of microscope during defrosting operation, do not simply fail to realize quick operating purpose, also very easily the defrosting effect of gamete or embryo is produced certain impact, eventually affect evolutive potentiality and the pregnancy outcome of gamete or embryo.
Summary of the invention
It is an object of the invention to provide a kind of gamete or embryo thawing method on freezing carrier, it has and can gamete on freezing carrier or embryo be thawed rapidly and accurately, thus improving the evolutive potentiality of gamete or embryo and the feature of pregnancy outcome.The invention still further relates to gamete on this freezing carrier or the vitrification used in embryo thawing method is thawed special ware.
First technical scheme of the present invention is:
Gamete or embryo thawing method on freezing carrier, described method comprises the following steps successively:
(1) the standby vitrification special ware of defrosting, this vitrification special ware of defrosting includes ware dish, ware lid, this ware dish is provided with the defrosting fluid apertures of upper opening, seen from above, this defrosting fluid apertures is isosceles triangle, and from the side, this defrosting fluid apertures is in less below and that straight flange is on right side right-angled trapezium, thus, the left side wall of this defrosting fluid apertures is inclined-plane, bottom is flat；
(2) in aforementioned defrosting fluid apertures, thawing solution is added；
(3) freezing load bar is adopted gamete or embryo to be taken out from liquid nitrogen, and gamete or embryo are positioned on the blade of this freezing load bar, make this blade move down sideling along the left side wall of this defrosting fluid apertures, make this blade be inserted to the bottom of this defrosting fluid apertures and be submerged in thawing solution；
(4) adopt microscope to observe that gamete or embryo drop to after among this defrosting fluid apertures, take this freezing load bar away, keep gamete or embryo at this defrosting fluid apertures inner equilibrium 60s；
(5) gamete or embryo's priority are moved into and complete gamete or embryo thawing on freezing carrier in diluent, washing liquid, culture fluid.
On the freezing carrier of the present invention, the advantage of gamete or embryo thawing method is:
Rapidly and accurately gamete on freezing carrier or embryo can be thawed, thus improving evolutive potentiality and the pregnancy outcome of gamete or embryo.Reason is in that: during freezing carrier is inserted directly into, along inclined-plane, the defrosting fluid apertures having added thawing solution, and period need not adjust microscope such that it is able to will be located in rapidly the gamete on blade or embryo comes off in defrosting fluid apertures, greatly improve work efficiency.
Second technical scheme of the present invention is:
The vitrification special ware of defrosting, including ware dish, ware lid, described ware dish is provided with the defrosting fluid apertures of upper opening, seen from above, this defrosting fluid apertures is isosceles triangle, and from the side, this defrosting fluid apertures is in less below and that straight flange is on right side right-angled trapezium, thus, the left side wall of this defrosting fluid apertures is inclined-plane, bottom is flat.
The vitrification of the present invention thaws special ware compared to the prior art advantageously: the blade inserted along inclined-plane when the bottom of defrosting fluid apertures and defrosting is substantially in same level, and required defrosting liquid measure is less, in operation without regulating microscope, it is favorably improved operating efficiency, therefore there is good practicality.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described:
Fig. 1 is the top view of the vitrification special ware of defrosting in embodiments of the invention 1 and 2；
Fig. 2 is the front sectional view of the vitrification special ware of defrosting in embodiments of the invention 1 and 2.
In figure:
10, ware dish；
20, defrosting fluid apertures, 21, left side wall, 22, bottom；
30, freezing load bar, 31, blade.
Detailed description of the invention
Embodiment 1
See shown in Fig. 1 and Fig. 2: the vitrification special ware of defrosting, for gamete on freezing carrier or embryo thawing.
Concretely, this special ware includes ware dish 10, ware lid (not shown on figure).This ware dish 10 is provided with the defrosting fluid apertures 20 of upper opening, and this ware lid is for covering in this defrosting fluid apertures 20 at ordinary times.Seen from above, this defrosting fluid apertures 20 is in isosceles triangle, it is preferred to equilateral triangle.From the side, this defrosting fluid apertures 20 is in less below and that straight flange is on right side right-angled trapezium.That is, the right angle of this right-angled trapezium is positioned at the lower right corner.Thus, the left side wall 21 of this defrosting fluid apertures 20 is that inclined-plane, bottom 22 are for flat.So, when to gamete on freezing carrier or embryo thawing, the thawing solution put in it is less, is favorably improved the efficiency of operation of thawing.
Embodiment 2
See shown in Fig. 1 and Fig. 2: gamete or embryo thawing method on freezing carrier, comprise the following steps:
(1) the standby vitrification special ware of defrosting.This vitrification special ware of defrosting is the special ware described in embodiment 1.
(2) in the defrosting fluid apertures 20 of this vitrification special ware of defrosting, thawing solution is added.
(3) freezing load bar 30 is adopted gamete or embryo to be taken out from liquid nitrogen, and gamete or embryo are positioned on the blade 31 of this freezing load bar 30, make this blade 31 move down sideling along the left side wall 21 of this defrosting fluid apertures 20, make this blade 31 be inserted to the bottom 22 of this defrosting fluid apertures 20 and be submerged in thawing solution.
(4) adopt microscope to observe that gamete or embryo drop to after among this defrosting fluid apertures 20, take this freezing load bar 30 away, keep gamete or embryo at this defrosting fluid apertures 20 inner equilibrium 60s.Such as.
(5) gamete or embryo's priority are moved into and complete gamete or embryo thawing on freezing carrier in diluent, washing liquid, culture fluid.
Through repeatedly testing, adopting on the freezing carrier of the present invention after gamete or embryo thawing method, thawing solution relatively commonsense method saves at least 50%, and defrosting efficiency improves at least 50%.Illustrate that the method adopting the present invention improves work efficiency, necessarily improve evolutive potentiality and the pregnancy outcome of gamete or embryo.
The foregoing is only the preferred embodiments of the present invention; not thereby the scope of the claims of the present invention is limited; every equivalent structure utilizing description of the present invention and accompanying drawing content to make or equivalence flow process conversion; or directly or indirectly it is used in other relevant technical fields, all in like manner include in the scope of patent protection of the present invention.
Claims (2)
1. gamete or embryo thawing method on freezing carrier, it is characterised in that: described method comprises the following steps successively:
(1) the standby vitrification special ware of defrosting, this vitrification special ware of defrosting includes ware dish, ware lid, this ware dish is provided with the defrosting fluid apertures of upper opening, seen from above, this defrosting fluid apertures is isosceles triangle, and from the side, this defrosting fluid apertures is in less below and that straight flange is on right side right-angled trapezium, thus, the left side wall of this defrosting fluid apertures is inclined-plane, bottom is flat；
(2) in aforementioned defrosting fluid apertures, thawing solution is added；
(3) freezing load bar is adopted gamete or embryo to be taken out from liquid nitrogen, and gamete or embryo are positioned on the blade of this freezing load bar, make this blade move down sideling along the left side wall of this defrosting fluid apertures, make this blade be inserted to the bottom of this defrosting fluid apertures and be submerged in thawing solution；
(4) adopt microscope to observe that gamete or embryo drop to after among this defrosting fluid apertures, take this freezing load bar away, keep gamete or embryo at this defrosting fluid apertures inner equilibrium 60s；
(5) gamete or embryo's priority are moved into and complete gamete or embryo thawing on freezing carrier in diluent, washing liquid, culture fluid.
2. the special ware of vitrification defrosting, including ware dish, ware lid, it is characterized in that: described ware dish is provided with the defrosting fluid apertures of upper opening, seen from above, this defrosting fluid apertures is isosceles triangle, and from the side, this defrosting fluid apertures is in less below and that straight flange is on right side right-angled trapezium, thus, the left side wall of this defrosting fluid apertures is inclined-plane, bottom is flat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242377.7A CN105707057B (en) | 2016-04-19 | 2016-04-19 | Gamete or embryo thawing method and the special ware of vitrifying defrosting on freezing carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610242377.7A CN105707057B (en) | 2016-04-19 | 2016-04-19 | Gamete or embryo thawing method and the special ware of vitrifying defrosting on freezing carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105707057A true CN105707057A (en) | 2016-06-29 |
| CN105707057B CN105707057B (en) | 2018-05-22 |
Family
ID=56160276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610242377.7A Expired - Fee Related CN105707057B (en) | 2016-04-19 | 2016-04-19 | Gamete or embryo thawing method and the special ware of vitrifying defrosting on freezing carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105707057B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632785A (en) * | 2021-08-17 | 2021-11-12 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203728851U (en) * | 2014-03-18 | 2014-07-23 | 于艳 | Vitrification refrigeration and resurrection culture plate |
| CN203851692U (en) * | 2014-06-01 | 2014-10-01 | 戴志俊 | Special vitrification vessel |
| CN205624137U (en) * | 2016-04-19 | 2016-10-12 | 戴志俊 | Glass dissolves and freezes special ware |
-
2016
- 2016-04-19 CN CN201610242377.7A patent/CN105707057B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203728851U (en) * | 2014-03-18 | 2014-07-23 | 于艳 | Vitrification refrigeration and resurrection culture plate |
| CN203851692U (en) * | 2014-06-01 | 2014-10-01 | 戴志俊 | Special vitrification vessel |
| CN205624137U (en) * | 2016-04-19 | 2016-10-12 | 戴志俊 | Glass dissolves and freezes special ware |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632785A (en) * | 2021-08-17 | 2021-11-12 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
| CN113632785B (en) * | 2021-08-17 | 2024-04-05 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105707057B (en) | 2018-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102669088B (en) | Mammal embryo and oocyte vitrification freezing carrier | |
| CN105707057A (en) | Method for unfreezing gamete or embryo on frozen carrier and vessel special for vitrification unfreezing | |
| CN205624137U (en) | Glass dissolves and freezes special ware | |
| CN110155514A (en) | A kind of vegetable logistics case of the metamorphosis heat absorption technology based on water | |
| CN102719427A (en) | Method for extracting DNA (deoxyribonucleic acid) by alkaline cracking | |
| CN201653784U (en) | Low-temperature device for impact test | |
| CN107352165B (en) | Access device of single sample box in liquid nitrogen jar | |
| CN205611617U (en) | Glass dissolves and freezes special ware | |
| CN204536096U (en) | Liquid nitrogen cryopreservation bucket | |
| CN201324729Y (en) | Automatic rinsing tank | |
| CN203385587U (en) | Novel sampling device | |
| CN201454550U (en) | Rock sample/soil sample chemical solution saturation device | |
| CN105494595A (en) | Quick-frozen food cooling pond | |
| CN102993979A (en) | Continuous turpentine clarifying tank device | |
| CN209338517U (en) | A kind of PCR pipe frame of simple competent cell liquid nitrogen flash freezer | |
| KR100712602B1 (en) | Micro pipet for removal of a nucleus from an egg | |
| CN204393831U (en) | Freezing serum thaw shelf | |
| CN210425693U (en) | Sample storage box for fish pathogen infection test | |
| CN203830283U (en) | Immersing suction tank | |
| CN203985845U (en) | A kind of lichee refrigeration automatic production line | |
| CN201404715Y (en) | Gas-liquid separator convenient for guiding liquid | |
| CN203529025U (en) | Blood cooling tank | |
| CN203516132U (en) | Intelligent centrifugal pump water injection device | |
| CN220376702U (en) | Protease cutting device | |
| CN205470281U (en) | Be used for frozen multilayer tray of rock specimen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180522 Termination date: 20190419 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |