CN105699658B - A kind of autism detection mark and its detection method - Google Patents
A kind of autism detection mark and its detection method Download PDFInfo
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Abstract
The present invention discloses a kind of autism detection mark and its detection method, one group of serum differential protein provided by the invention detects available for autism, when the serum differential protein is used for autism detection, the specificity and accuracy of detection can be improved, there is certain objectivity simultaneously, better than single disease marker.The present invention determines to whether there is serum differential protein in serum using high throughput proteomics method, and these albumen are carried out to detect one by one by elisa technique or these albumen detect simultaneously using protein chip technology, it is determined that the change in concentration of the serum differential protein detected, compared with existing detection method (autism Survey), detection method specificity, sensitivity and accuracy are high.
Description
Technical field
The present invention relates to disease detection technical field, more particularly to a kind of autism detection mark and its detection method.
Background technology
Autism is also known as self-closing disease, is a kind of nervous system disorder disease, and the incidence of disease increases year by year.Due to the cause of disease
And pathogenesis not yet illustrates, at present without specific treatment medicine and specific detection mark, but early discovery, early intervention can be obvious
Improve detection and treatment.Detection Main Basiss scale, clinical symptoms, patient or the family members of current autism describe, clinician
Clinical experience etc., there is certain subjectivity, easily cause detection delay or mistaken diagnosis.
Therefore, prior art has yet to be improved and developed.
The content of the invention
In view of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of autism detection mark and its inspection
Survey method, it is intended to which solving existing detection method has certain subjectivity, easily causes the problem of detecting delay or mistaken diagnosis.
Technical scheme is as follows：
A kind of autism detects mark, wherein, the autism detection mark is Autism children and normal healthy controls
Serum differential protein, the serum differential protein is by actin, α -1- antitrypsins, α-actin -1, α-enol
Change enzyme, proangiotensin, Antithrombin Ⅲ, apolipoprotein 1, apolipoprotein 4, apo E, β-cellule albumen, calcium
Heregulin, calreticulin, Complement C_3, complement C5, EH regions include albumen, ferritin family homolog 3, fibronectin, fibre
Albumen -1, integrin alpha-IIb, a α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolk 2, turn
Ferritin, Src substrate skins filamentous actin, talin -1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, stick together
Spot albumen, vitronectin and vitamin D binding protein are formed.
A kind of detection method based on autism as described above detection mark, wherein, using high throughput protein group
Method determines serum differential protein as described above whether is deposited in serum.
The detection method of the autism detection mark, wherein it is determined that also being wrapped after serum differential protein in serum being present
Include：Serum differential protein is detected one by one using Enzyme-multiplied immune technique, it is determined that the concentration of the serum differential protein detected
Change.
The detection method of the autism detection mark, wherein it is determined that also being wrapped after serum differential protein in serum being present
Include：Serum differential protein is detected one by one using protein chip technology, it is determined that the concentration of the serum differential protein detected
Change.
Beneficial effect：The above-mentioned serum differential protein of the present invention detects available for autism, and above-mentioned 29 differential proteins are made
When being used for autism detection for one group of serum differential protein, the specificity and accuracy of detection can be improved, while is had certain
Objectivity, better than single disease marker.
Embodiment
The present invention provides a kind of autism detection mark and its detection method, to make the purpose of the present invention, technical scheme
And effect is clearer, clear and definite, the present invention is described in more detail below.It should be appreciated that specific implementation described herein
Example is not intended to limit the present invention only to explain the present invention.
The present invention provides a kind of autism detection mark, wherein, the autism detection mark is Autism children
With the serum differential protein of normal healthy controls, the serum differential protein moves egg by actin, α -1- antitrypsins, α-flesh
In vain -1, α-enolase, proangiotensin, Antithrombin Ⅲ, apolipoprotein 1, apolipoprotein 4, apo E, β-small
Cell protein, calmodulin, calreticulin, Complement C_3, complement C5, EH regions include albumen, ferritin family homolog 3,
Fibronectin, fine albumen -1, integrin alpha-IIb, a α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB
Kinsfolk 2, transferrins, Src substrate skins filamentous actin, talin -1, THBS1, transition endoplasmic reticulum adenosine three
Phosphatase, vinculin, vitronectin and vitamin D binding protein are formed.
Blood can be reflected conveniently and preferably body pathology physiology course and preferably come as disease marker due to sampling
One of source.But search out specificity and accuracy is high, the serum protein markers of clinical detection can be used for, acquired a certain degree of difficulty.
Previously research shows that autism belongs to multifactor relevant disease.Therefore, autism detection mark, especially haemocyanin are being found
During mark, it is difficult to search out single serum protein markers.Therefore, the present invention provides above-mentioned serum differential protein and (amounted to
29) it is used for autism detection, and above-mentioned 29 serum differential proteins are used for as one group of (or series) serum differential protein
Autism detects, and takes the mode of the composite marker thing to improve the specificity and accuracy of detection, while have certain visitor
The property seen, better than single disease marker.It should be noted that any three kinds in the above-mentioned differential protein of the present invention (amounting to 29) or
More than three kinds albumen can be used as autism detection mark, any three kinds or three kinds in above-mentioned differential protein (amounting to 29)
When above albumen is used to detect autism as autism detection mark, equally with certain objectivity, specificity and standard
True property.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
In actin, α -1- antitrypsins, α-actin -1.Above-mentioned albumen can be used as autism detection mark, be used for
Autism equally has certain objectivity, specificity and accuracy when detecting.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
In actin, α -1- antitrypsins, α-actin -1, α-enolase, proangiotensin, Antithrombin Ⅲ.
Above-mentioned albumen can be used as autism detection mark, equally have certain objectivity, specificity when being detected for autism
And accuracy.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
In apolipoprotein 1, calreticulin and Complement C_3.Above-mentioned albumen can be used as autism detection mark, be examined for autism
Equally there is certain objectivity, specificity and accuracy during survey.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
In apolipoprotein 1, calmodulin, calreticulin, fibronectin, fine albumen -1 and integrin alpha-IIb.Above-mentioned albumen equally may be used
Mark is detected as autism, equally there is certain objectivity, specificity and accuracy when being detected for autism.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
Ferritin family homolog 3, fibronectin, fine albumen -1, integrin alpha-IIb, a α-trypsin ihhibitor heavy chain H1,
Microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skins filamentous actin, talin -1 and platelet response egg
In vain -1.Above-mentioned albumen equally can be used as autism detection mark, when detect for autism equally with certain objectivity,
Specificity and accuracy.
As the preferred embodiment of the present invention, the present invention can determine whether deposited in serum using high throughput protein group method
Albumen, ferritin family homolog 3, fibre are included in apolipoprotein 4, apo E, β-cellule albumen, complement C5, EH regions
Even albumen, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skins filamentous actin, talin -1, blood platelet
Reactive protein -1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and vitamin D binding protein.Above-mentioned egg
Autism detection mark can be equally used as in vain, and equally there is certain objectivity, specificity and standard when being detected for autism
True property.
Mark is detected based on above-mentioned autism, the present invention also provides a kind of based on autism as described above detection mark
Detection method, wherein, determine to whether there is serum difference as described above in serum using high throughput protein group method
Albumen.The present invention determines to whether there is above-mentioned 29 differential proteins in serum using high throughput protein group method, and works as blood
When above-mentioned 29 differential proteins be present in clear, there is up-regulated expression protein 11 in above-mentioned 29 differential proteins, express down-regulation protein
18.
Further, it is determined that also include after serum differential protein in serum being present：It is poor to serum using Enzyme-multiplied immune technique
M-band is detected one by one, it is determined that the change in concentration of the serum differential protein detected.Using high throughput protein group
Method determines that above-mentioned 29 differential proteins in serum be present (has up-regulated expression protein 11, under expression in 29 differential proteins
Heregulin 18) after, above-mentioned serum differential protein is detected one by one using Enzyme-multiplied immune technique (ELISA), it is determined that detection
The change in concentration of the serum differential protein arrived.Compared with existing autism detection method, autism detection method tool of the present invention
There are specificity, sensitivity and the high advantage of accuracy.
Further, present invention determine that also including after serum differential protein in serum being present：Using protein chip technology pair
Serum differential protein is detected one by one, it is determined that the change in concentration of the serum differential protein detected.Using high flux egg
White matter group method determine to exist in serum above-mentioned 29 differential proteins (have up-regulated expression protein 11 in 29 differential proteins,
Express down-regulation protein 18) after, the present invention can also be detected one by one using protein chip technology to serum differential protein, really
The change in concentration for the serum differential protein that regular inspection measures.Compared with existing autism detection method, autism detection of the present invention
Method is with the high advantage of specific, sensitivity and accuracy.
Below by specific embodiment, the present invention is described in detail.
Embodiment 1
Autism children serum and healthy children serum are gathered respectively, and autism is suffered from using high throughput proteomics method
Youngster's serum and healthy children serum are compared analysis.As a result obtain：Compared with compareing healthy children serum, in Autism children
Serum differential protein 29 is obtained in serum, wherein 11 expressing protein up-regulations, 18 expressing proteins are lowered, refers to table 1 below.
Then 29 differential proteins are detected one by one using Enzyme-multiplied immune technique, or using protein chip technology to 29 differences
Albumen is detected one by one, it is determined that the change in concentration of the serum differential protein detected.The present invention passes through high throughput proteomics
Method, by being analyzed compared with healthy children serum Autism children serum, compared with the control, obtain serum difference
Protein 29, up-regulated expression protein 11, down-regulation protein 18 is expressed, and these albumen are carried out one by one by elisa technique
Detection to these albumen detect simultaneously using protein chip technology, it is determined that the concentration of the serum differential protein detected becomes
Change.Compared with existing detection method (autism Survey), detection method specificity, sensitivity and accuracy
It is high.
Table 1, autism children and healthy children serum differential protein
In summary, a kind of autism detection mark of present invention offer and its detection method, one group provided by the invention
Serum differential protein detects available for autism when being detected for autism, can improve the specificity and accuracy of detection, simultaneously
With certain objectivity, better than single disease marker.The present invention uses high throughput proteomics method, by autism
Infant is analyzed compared with healthy children serum, obtains the two serum differential protein 29, compared with the control, up-regulated expression
Protein 11, down-regulation protein 18 is expressed, and these albumen are carried out to detect one by one or using protein chip by elisa technique
Technology to these albumen detect simultaneously, it is determined that the change in concentration of the serum differential protein detected, with existing detection side
Method (autism Survey) is compared, and detection method specificity, sensitivity and accuracy are high.
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can
To be improved or converted according to the above description, all these modifications and variations should all belong to the guarantor of appended claims of the present invention
Protect scope.
Claims (4)
1. one group of serum differential protein is preparing the application in being used to detect the reagent of autism, it is characterised in that the serum
Differential protein is by actin, α -1- antitrypsins, α-actinine -1, α-enolase, proangiotensin, anti-freezing
Hemase-III, Apolipoprotein A1, Apo A4, apo E, β-cellule albumen, calmodulin, calreticulin, benefit
Body C3, complement C5, EH regions include albumen 3, ferritin family homolog 3, fibronectin, fine albumen -1, integrin alpha-IIb,
Between α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolks 2, transferrins, Src substrate skins
Filamentous actin, talin -1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and
Vitamin D binding protein is formed；
α -1- the antitrypsins, proangiotensin, Antithrombin Ⅲ, Apo A4, apo E, Complement C_3,
Complement C5, fibronectin, fine albumen -1, a α-trypsin ihhibitor heavy chain H1, vitronectin should in the detection of autism
With middle up-regulated expression；
The actin, α-actinine -1, α-enolase, Apolipoprotein A1, β-cellule albumen, calmodulin,
Calreticulin, EH regions include albumen 3, ferritin family homolog 3, integrin alpha-IIb, microtubule-associated proteins RP/
EB kinsfolk 2, transferrins, Src substrate skins filamentous actin, talin -1, THBS1, transition endoplasmic reticulum gland
Guanosine triphosphate enzyme, vinculin, vitamin D binding protein are expressed in the detection application of autism and lowered.
2. application according to claim 1, it is characterised in that determined using high throughput protein group method be in serum
The no serum differential protein existed described in claim 1.
3. application according to claim 2, it is characterised in that determine also to include after serum differential protein in serum being present：
Serum differential protein is detected one by one using Enzyme-multiplied immune technique, it is determined that the concentration of the serum differential protein detected becomes
Change.
4. application according to claim 2, it is characterised in that determine also to include after serum differential protein in serum being present：
Serum differential protein is detected one by one using protein chip technology, it is determined that the concentration of the serum differential protein detected becomes
Change.
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| CN106093441B (en) * | 2016-07-27 | 2017-10-20 | 西安交通大学 | A kind of purposes of autism seroglycoid label |
| CN109813912B (en) * | 2019-01-04 | 2021-12-28 | 深圳大学 | Application of group of serum differential protein combinations in preparation of reagent for detecting autism |
| CN109608534B (en) * | 2019-01-23 | 2019-08-09 | 东北师范大学 | A kind of cortical actin mutant 8KQ and application |
| CN109946447A (en) * | 2019-03-13 | 2019-06-28 | 深圳大学 | A kind of diagnosis marker for detecting autism-spectrum obstacle, equipment and application |
| KR102415395B1 (en) * | 2020-09-09 | 2022-06-30 | 한국생명공학연구원 | Method for diagnosing early neurodevelopmental disorder using biomarker and kit using the same |
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| EP2490028B1 (en) * | 2009-10-14 | 2016-11-30 | National University Corporation Hamamatsu University School of Medicine | Method and marker for determination of degree of risk of onset of high-functioning autism |
| CN103290135A (en) * | 2013-06-26 | 2013-09-11 | 北京迈基诺基因科技有限责任公司 | Screening method of infantile autism gene |
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