CN105699658A - Autism detection marker and detection method thereof - Google Patents
Autism detection marker and detection method thereof Download PDFInfo
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Abstract
The invention discloses an autism detection marker and a detection method thereof. A set of serum differential proteins provided by the invention can be utilized to detect autism. When the serum differential proteins are used for detecting autism, the detection specificity and accuracy can be promoted, and meanwhile, the result is objective, and the autism detection marker is better than a single disease marker. A high-flux proteomics method is adopted for confirming if the serum differential proteins exist in serum; ELISA technique is adopted for detecting these proteins one by one or a protein chip technique is adopted for detecting these proteins at the same time; the concentration change of the detected serum differential proteins can be confirmed. Compared with the present detection method (autism detection table), the detection method provided by the invention has the advantages of high specificity, sensitivity and accuracy.
Description
Technical field
The present invention relates to disease detection technical field, particularly relate to a kind of autism detection mark and detection method thereof。
Background technology
Autism, also known as infantile autism, is a kind of nervous system disorder disease, and sickness rate increases year by year。Owing to the cause of disease and pathogenesis not yet illustrate, at present without specific treatment medicine and specific detection mark, but early discovery, early intervention can obviously improve detection and treatment。Current autistic detection Main Basis scale, clinical symptoms, patient or family members describe, the clinical experience etc. of clinician, have certain subjectivity, easily cause detection and postpone or mistaken diagnosis。
Therefore, prior art has yet to be improved and developed。
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of autism detection mark and detection method thereof, it is intended to solve existing detection method and there is certain subjectivity, the problem easily causing detection delay or mistaken diagnosis。
Technical scheme is as follows:
A kind of autism detection mark, wherein, described autism detection mark is the serum differential protein of Autism children and normal healthy controls, described serum differential protein is by actin, α-1-antitrypsin, α-actin-1, α-Enolase, proangiotensin, Antithrombin Ⅲ, apolipoprotein 1, apolipoprotein 4, apo E, β-minicell albumen, calmodulin, CaM, calreticulin, Complement C_3, complement C5, EH region comprises albumen, ferritin family homolog 3, fibronectin, fine albumen-1, integrin alpha-IIb, between α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skin filamentous actin, talin-1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and vitamin D binding protein are constituted。
A kind of detection method detecting mark based on autism described above, wherein, adopts high throughput protein group method determines whether deposit serum differential protein as above in serum。
The detection method of described autism detection mark, wherein it is determined that also include after there is serum differential protein in serum: adopt Enzyme-multiplied immune technique that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。
The detection method of described autism detection mark, wherein it is determined that also include after there is serum differential protein in serum: adopt protein chip technology that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。
Beneficial effect: the above-mentioned serum differential protein of the present invention can be used for autism detection, and above-mentioned 29 differential proteins as one group of serum differential protein for autism detection time, specificity and the accuracy of detection can be improved, there is certain objectivity simultaneously, be better than single disease marker。
Detailed description of the invention
The present invention provides a kind of autism detection mark and detection method thereof, and for making the purpose of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below。Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention。
The present invention provides a kind of autism detection mark, wherein, described autism detection mark is the serum differential protein of Autism children and normal healthy controls, described serum differential protein is by actin, α-1-antitrypsin, α-actin-1, α-Enolase, proangiotensin, Antithrombin Ⅲ, apolipoprotein 1, apolipoprotein 4, apo E, β-minicell albumen, calmodulin, CaM, calreticulin, Complement C_3, complement C5, EH region comprises albumen, ferritin family homolog 3, fibronectin, fine albumen-1, integrin alpha-IIb, between α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skin filamentous actin, talin-1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and vitamin D binding protein are constituted。
Blood is owing to sampling convenient and can better reflect that body pathology physiological process becomes preferably one of source of disease marker。But search out specificity and accuracy is high, the serum protein markers of Clinical detection can be used for, acquire a certain degree of difficulty。Previously research shows that autism belongs to multifactor relevant disease。Therefore, finding autism detection mark, especially during serum protein markers, it is difficult to search out single serum protein markers。Therefore, the present invention provides above-mentioned serum differential protein (amounting to 29) to detect for autism, and above-mentioned 29 serum differential proteins as one group of (or claiming series) serum differential protein for autism detection, the mode taking this composite marker thing can improve specificity and the accuracy of detection, there is certain objectivity simultaneously, be better than single disease marker。It should be noted that, any three kinds or more albumen in the above-mentioned differential protein of the present invention (altogether 29) can be used as autism detection mark, any three kinds or more albumen in above-mentioned differential protein (amount to 29) as autism detect mark be used for detecting autism time, there is certain objectivity, specificity and accuracy equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether there is actin in serum, α-1-antitrypsin, α-actin-1。Above-mentioned albumen can be used as autism detection mark, has certain objectivity, specificity and accuracy for autism when detecting equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether there is actin in serum, α-1-antitrypsin, α-actin-1, α-Enolase, proangiotensin, Antithrombin Ⅲ。Above-mentioned albumen can be used as autism detection mark, has certain objectivity, specificity and accuracy for autism when detecting equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether to there is apolipoprotein 1, calreticulin and Complement C_3 in serum。Above-mentioned albumen can be used as autism detection mark, has certain objectivity, specificity and accuracy for autism when detecting equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether there is apolipoprotein 1 in serum, calmodulin, CaM, calreticulin, fibronectin, fine albumen-1 and integrin alpha-IIb。Above-mentioned albumen can detect mark as autism equally, has certain objectivity, specificity and accuracy for autism when detecting equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether there is ferritin family homolog 3 in serum, fibronectin, fine albumen-1, integrin alpha-IIb, a α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skin filamentous actin, talin-1 and THBS1。Above-mentioned albumen can detect mark as autism equally, has certain objectivity, specificity and accuracy for autism when detecting equally。
As the preferred embodiment of the present invention, the present invention can adopt high throughput protein group method to determine whether there is apolipoprotein 4 in serum, apo E, β-minicell albumen, complement C5, EH region comprise albumen, ferritin family homolog 3, fibronectin, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skin filamentous actin, talin-1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and vitamin D binding protein。Above-mentioned albumen can detect mark as autism equally, has certain objectivity, specificity and accuracy for autism when detecting equally。
Detecting mark based on above-mentioned autism, the present invention also provides for a kind of detection method detecting mark based on autism described above, wherein, adopts high throughput protein group method determines whether there is serum differential protein as above in serum。The present invention adopts high throughput protein group method to determine whether to there are above-mentioned 29 differential proteins in serum, and when there is above-mentioned 29 differential proteins in serum, has up-regulated protein 11 in above-mentioned 29 differential proteins, down-regulated expression protein 18。
Further, it is determined that also include after serum exists serum differential protein: adopt Enzyme-multiplied immune technique that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。Namely determine that there are above-mentioned 29 differential proteins in serum (has up-regulated protein 11 in 29 differential proteins in employing high throughput protein group method, down-regulated expression protein 18) after, adopt Enzyme-multiplied immune technique (ELISA) that above-mentioned serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。Compared with existing autism detection method, autism detection method of the present invention has the advantage that specificity, sensitivity and accuracy are high。
Further, present invention determine that in serum and also include after there is serum differential protein: adopt protein chip technology that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。Namely determine that there are above-mentioned 29 differential proteins in serum (has up-regulated protein 11 in 29 differential proteins in employing high throughput protein group method, down-regulated expression protein 18) after, the present invention can also adopt protein chip technology that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。Compared with existing autism detection method, autism detection method of the present invention has the advantage that specificity, sensitivity and accuracy are high。
Below by specific embodiment, the present invention is described in detail。
Embodiment 1
Gather Autism children serum and healthy children serum respectively, adopt high throughput proteomics method that Autism children serum and healthy children serum are compared analysis。Result obtains: compared with comparison healthy children serum, obtain serum differential protein 29 in Autism children serum, and wherein 11 expressing proteins raise, and 18 expressing proteins are lowered, and refer to table 1 below。Then adopt Enzyme-multiplied immune technique that 29 differential proteins are detected one by one, or adopt protein chip technology that 29 differential proteins are detected one by one, it is determined that the concentration change of the serum differential protein detected。The present invention passes through high throughput proteomics method, by Autism children serum and healthy children serum are compared analysis, compared with the control, obtain serum differential protein 29, up-regulated protein 11, down-regulated expression protein 18, and carry out detecting or adopt protein chip technology that these albumen are detected simultaneously one by one to these albumen by elisa technique, it is determined that the concentration change of the serum differential protein detected。Compared with existing detection method (autism Survey), detection method specificity, sensitivity and accuracy are high。
Table 1, autistic children belong and healthy children serum differential protein
In sum, the present invention provides a kind of autism detection mark and detection method thereof, and one group of serum differential protein provided by the invention can be used for autism detection when detect for autism, can improve the specificity and accuracy that detect, there is certain objectivity simultaneously, be better than single disease marker。The present invention adopts high throughput proteomics method, by Autism children and healthy children serum are compared analysis, obtain the two serum differential protein 29, compared with the control, up-regulated protein 11, down-regulated expression protein 18, and carry out detecting or adopt protein chip technology that these albumen are detected simultaneously one by one to these albumen by elisa technique, determine the concentration change of the serum differential protein detected, compared with existing detection method (autism Survey), detection method specificity, sensitivity and accuracy are high。
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, it is possible to improved according to the above description or convert, all these improve and convert the protection domain that all should belong to claims of the present invention。
Claims (4)
1. an autism detection mark, it is characterized in that, described autism detection mark is the serum differential protein of Autism children and normal healthy controls, described serum differential protein is by actin, α-1-antitrypsin, α-actin-1, α-Enolase, proangiotensin, Antithrombin Ⅲ, apolipoprotein 1, apolipoprotein 4, apo E, β-minicell albumen, calmodulin, CaM, calreticulin, Complement C_3, complement C5, EH region comprises albumen, ferritin family homolog 3, fibronectin, fine albumen-1, integrin alpha-IIb, between α-trypsin ihhibitor heavy chain H1, microtubule-associated proteins RP/EB kinsfolk 2, transferrins, Src substrate skin filamentous actin, talin-1, THBS1, transition endoplasmic reticulum adenosine triphosphatase, vinculin, vitronectin and vitamin D binding protein are constituted。
2. the detection method detecting mark based on autism described in claim 1, it is characterised in that adopt high throughput protein group method to determine the serum differential protein whether existed in serum described in claim 1。
3. autism detects the detection method of mark according to claim 2, it is characterized in that, determine and also include after serum exists serum differential protein: adopt Enzyme-multiplied immune technique that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。
4. autism detects the detection method of mark according to claim 2, it is characterized in that, determine and also include after serum exists serum differential protein: adopt protein chip technology that serum differential protein is detected one by one, it is determined that the concentration change of the serum differential protein detected。
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Cited By (5)
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| CN106093441A (en) * | 2016-07-27 | 2016-11-09 | 西安交通大学 | A kind of purposes of autism seroglycoid label |
| CN109608534A (en) * | 2019-01-23 | 2019-04-12 | 东北师范大学 | A kind of cortical actin mutant 8KQ and application |
| CN109813912A (en) * | 2019-01-04 | 2019-05-28 | 深圳大学 | One group of serum differential protein combination is preparing the application in the reagent for detecting autism |
| CN109946447A (en) * | 2019-03-13 | 2019-06-28 | 深圳大学 | A kind of diagnosis marker for detecting autism-spectrum obstacle, equipment and application |
| KR20220033346A (en) * | 2020-09-09 | 2022-03-16 | 한국생명공학연구원 | Method for diagnosing early neurodevelopmental disorder using biomarker and kit using the same |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106093441A (en) * | 2016-07-27 | 2016-11-09 | 西安交通大学 | A kind of purposes of autism seroglycoid label |
| CN109813912A (en) * | 2019-01-04 | 2019-05-28 | 深圳大学 | One group of serum differential protein combination is preparing the application in the reagent for detecting autism |
| CN109813912B (en) * | 2019-01-04 | 2021-12-28 | 深圳大学 | Application of group of serum differential protein combinations in preparation of reagent for detecting autism |
| CN109608534A (en) * | 2019-01-23 | 2019-04-12 | 东北师范大学 | A kind of cortical actin mutant 8KQ and application |
| CN109946447A (en) * | 2019-03-13 | 2019-06-28 | 深圳大学 | A kind of diagnosis marker for detecting autism-spectrum obstacle, equipment and application |
| KR20220033346A (en) * | 2020-09-09 | 2022-03-16 | 한국생명공학연구원 | Method for diagnosing early neurodevelopmental disorder using biomarker and kit using the same |
| KR102415395B1 (en) * | 2020-09-09 | 2022-06-30 | 한국생명공학연구원 | Method for diagnosing early neurodevelopmental disorder using biomarker and kit using the same |
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