CN105699371A - Reagent for detecting aldehyde contained in liquid and preparation method - Google Patents
Reagent for detecting aldehyde contained in liquid and preparation method Download PDFInfo
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- CN105699371A CN105699371A CN201610058126.3A CN201610058126A CN105699371A CN 105699371 A CN105699371 A CN 105699371A CN 201610058126 A CN201610058126 A CN 201610058126A CN 105699371 A CN105699371 A CN 105699371A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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Abstract
The invention provides a reagent for detecting aldehyde contained in liquid and a preparation method. The reagent comprises the following components by weight part: 14.4-16.7 parts of sodium thiosulfate, 7.2-8.3 parts of phosphoric acid, 2.1-2.7 parts of basic fuchsin and 972.2-976.4 parts of deionized water. The method comprises the following steps: 1) preparing raw solutions; 2) de-coloring and performing standing reaction; 3) adjusting pH value; 4) preparing the reagent. The reagent provided by the invention is the reagent only containing one basic fuchsin and capable of quickly reacting with aldehyde; a nitrogen protecting layer is established on a detection reagent, any oxidizing reaction of the reagent can be stopped and the guarantee period can be prolonged; the prepared portable kit can be easily operated and is portable; the aldehyde concentrations in samples can be compared and judged by utilizing a colourimetric card after developing, so as to evaluate the oxidative stress level.
Description
Technical field
The present invention relates to chemical field, be related specifically to a kind of reagent and preparation method detecting and containing aldehyde in liquid。
Background technology
Oxidative stress (OxidativeStress) refers to that vivo oxidation and antioxidation are unbalance, produces a large amount of intermediate oxidation product, is a kind of negative effect produced in vivo by free radical, it is believed that be cause old and feeble and disease a key factor。Now increasing data show, oxidative stress and the disease association such as cardiovascular and cerebrovascular disease, diabetes。When animal or plant cell generation oxidative stress, lipid oxidation can be there is, and malonaldehyde (Malondialdehyde, MDA) natural product of a kind of organism lipid oxidation it is identified as, can be released in urine, therefore can judge vivo oxidation stress level by the MDA in detection urine。Malonaldehyde (MDA) is taken as the metabolite of lipid oxidation。Aldehyde quantitatively can pass through use equipment such as spectrophotometer, fluoremetry, high performance liquid chromatograph, gas chromatography mass spectrometer etc. detect, the existence of aldehyde can also be measured by other method, but the more applicable laboratory of these methods uses, be not suitable for individual among the people quickly and easily low cost and judge。Also some are had to be suitable for quickly and easily measuring the method invention of aldehyde radical or technology, but these inventions or the total reagent buffer capacity haveing the drawback that used by detection of technology are inadequate, false-positive result is there will be, because colour developing is relevant to final pH value for detecting the sample as the more inherently buffer of the content of organic matter such as urine, perspiration。And market lacks the MDA easy detection test kit that buffer itself ability is stronger。
Aldehyde is also present in diet, such as Chinese liquor etc., and in fixing foaming seafood (formaldehyde), therefore can be instructed the healthy living of people by the detection of aldehyde。Aldehyde quantitatively can pass through use equipment such as spectrophotometer, fluoremetry, high performance liquid chromatograph, gas chromatography mass spectrometer etc. detect, it is also possible to measured the existence of aldehyde by other method。Such as, F.Feigl is at ElsevierPublishingCompany, Ind. " QualitativeAnalysisBySpotTests " published, 3rd edition, described in 395 pages, a droplet sample solution is instilled in the test tube containing 2ml72% sulphuric acid, mix homogeneously, it is subsequently adding a small amount of solid ChromatropicAcid (1,8-Dihydrooxynapthianene-36disulfate),, if there being the existence of aldehyde in sample, there is brilliant violet color in the water-bath of 60 DEG C about 10 minutes, it is said the aldehyde that sensitivity is about 3ppm of this test。
Feigl describes the detection method of another kind of aldehyde at publication 339-340 page, droplet water being likely to containing aldehyde (or alcohol) solution example is dropped on spot plate, then mix with a sulfurous acid and a magenta/sulfuric acid solution, in about 2 to 30 minutes, mixed liquor shows red to blue, and the degree of depth of mixed liquor color is relevant to the amount of the aldehyde existed in detected solution。It is reported that this detection method can detect the formaldehyde of about 1 microgram in a sample。The detection method reaction of above aldehyde operates not rapidly and not easily, for instance first method needs to heat test tube solution 10 minutes in water bath with thermostatic control, therefore urgently seeks a kind of simple method。
Utilizing the invention that basic fuchsin reagent detection aldehyde exists to also have a lot, one of them is a kind of aldehyde detection method disclosed in U.S. Patent No. 6,165,797。This detectable comprises about 90-110 part 20% acetic acid, (solution A is by the sodium pyrosulfite of 18-22 gram for the solution B of the solution A of about 13.5-16.5 part and about 4.5-5.5 part, the acid of 9-11 milliliter strong phosphoric acid and 450-550 ml deionized water composition, solution B is dissolved in about 90-110 milliliter reagent A by 0.45-0.55 gram of basic fuchsin and forms)。Acidity is too strong because of the acetic acid used to have been found that this reagent, and it is very fast to be added thereto to urine the reaction time, causes occurring color to change due to urine pH value。Therefore false positive occurs。The patent of invention also having an invention to be domestic publication number CN100338463C, according to its statement, first sodium pyrosulfite is about 8-12g, phosphatase 11 0ml, basic fuchsin is about 4.8-5.2g, it is dissolved in the deionized water of about 995-1005ml, then takes 0.1ml mentioned reagent and 1ml sample mix, judge the level of the MDA existed in urine according to chromogenic reaction。Because phosphoric acid acid used is relatively strong, during for urine detection, buffer capacity is inadequate, and basic fuchsin colour developing is not only correlated with aldehyde, is also correlated with solution ph, so testing result also there will be false positive。If can not by pH of mixed value stabilization a less scope, it is impossible to unanimously and reliably detecting the existence of aldehyde in sample, the accuracy of detection is also subjected to challenge。
It is thus desirable to the reagent of a kind of improvement is for detecting the aldehyde of trace in urine or food, for instructing living habit and the health diet of people, reduce the generation of disease, and this detection can individual independently be conveniently accomplished。
Summary of the invention
It is an object of the invention to provide a kind of reagent and preparation method detecting and containing aldehyde in liquid, its purpose one is the aldehyde of trace in detection urine or goods, for instructing living habit and the health diet of people；Purpose two is for avoiding false-positive appearance in testing result。
For achieving the above object, the technical scheme is that
A kind of detect in liquid containing the reagent of aldehyde, it is characterised in that: described reagent includes the component of following parts by weight of component:
Sodium thiosulfate is about 14.4-16.7 part；Phosphoric acid is about 7.2-8.3 part；Basic fuchsin is about 2.1-2.7 part；Deionized water 972.2-976.4 part。
A kind of preparation method detecting the reagent containing aldehyde in liquid, it is characterised in that: described method carries out as follows:
1, preparation stock solution: the deionized water that parts by weight of component is 972.2-976.4 part is injected in container, take the basic fuchsin that parts by weight of component is 2.1-2.7 part and the sodium thiosulfate that parts by weight of component is 14.4-16.7 part is dissolved in the deionized water that parts by weight of component is 972.2-976.4 part, it is subsequently adding the phosphoric acid that parts by weight of component is 7.2-8.3 part, it is sufficiently mixed 10-30 minute, makes stock solution standby；
2, decolouring and standing and reacting: the stock solution of preparation in step 1 is injected in hermetic container, it is simultaneously introduced the activated carbon decolorizing of 20-30g, at normal temperatures and pressures, seals lucifuge and place 24-36 hour, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
3, pH value is regulated: the solution obtained in step 2 is slowly added to citric acid solution, and by the pH regulator of solution to 1.95-2.15, makes reagent；
4, prepare test kit: encapsulated with 0.1-0.3ml dosage ampoule bottle nitrogen charging by the reagent of gained in step 3, be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
The positive effect of the present invention is:
1, the present invention is containing only the reagent having a kind of basic fuchsin, rapid with aldehyde reaction, and establishes a nitrogen blanket in detectable, prevents any oxidation reaction of this reagent, extends the shelf-life；
2, Portable kit prepared by the present invention, it is easy to operation, and easy to carry, utilize the colorimetric card after colour developing to compare the height of aldehyde concentration in judgement sample, thus evaluating oxidative stress level；
3, the present invention avoids false-positive appearance in the detection。
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described。
Embodiment one
1, the deionized water that parts by weight of component is 972.2 parts is injected in container, take the basic fuchsin that parts by weight of component is 2.1 parts and the sodium thiosulfate that parts by weight of component is 14.4 parts is dissolved in wherein, it is subsequently adding the phosphoric acid that parts by weight of component is 7.2 parts, is sufficiently mixed 10 minutes, makes stock solution standby；
2, the stock solution of preparation in step 1 is injected in hermetic container, is simultaneously introduced the activated carbon decolorizing of 20g, at normal temperatures and pressures, seal lucifuge and place 24 hours, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
3, the solution obtained in step 2 is slowly added to citric acid solution, and by the pH regulator of solution to 1.95, makes reagent；
4, the reagent of gained in step 3 is encapsulated with 0.1ml dosage ampoule bottle or other similar containers nitrogen charging, be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
Embodiment two
1, the deionized water that parts by weight of component is 974 parts is injected in container, take the basic fuchsin that parts by weight of component is 2.3 parts and the sodium thiosulfate that parts by weight of component is 15 parts is dissolved in wherein, it is subsequently adding the phosphoric acid that parts by weight of component is 7.5 parts, is sufficiently mixed 15 minutes, makes stock solution standby；
2, the stock solution of preparation in step 1 is injected in hermetic container, is simultaneously introduced the activated carbon decolorizing of 24g, at normal temperatures and pressures, seal lucifuge and place 28 hours, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
3, the solution obtained in step 2 is slowly added to citric acid solution, and by the pH regulator of solution to 1.95, makes reagent；
4, the reagent of gained in step 3 is encapsulated with 0.2ml dosage ampoule bottle or other similar containers nitrogen charging, be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
Embodiment three
1, the deionized water that parts by weight of component is 975 parts is injected in container, take the basic fuchsin that parts by weight of component is 2.5 parts and the sodium thiosulfate that parts by weight of component is 16 parts is dissolved in wherein, it is subsequently adding the phosphoric acid that parts by weight of component is 7.8 parts, is sufficiently mixed 20 minutes, makes stock solution standby；
2, the stock solution of preparation in step 1 is injected in hermetic container, is simultaneously introduced the activated carbon decolorizing of 26g, at normal temperatures and pressures, seal lucifuge and place 32 hours, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
3, the solution obtained in step 2 is slowly added to citric acid solution, and by the pH regulator of solution to 2.0, makes reagent；
4, the reagent of gained in step 3 is encapsulated with 0.3ml dosage ampoule bottle or other similar containers nitrogen charging, be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
Embodiment four
1, the deionized water that parts by weight of component is 976.4 parts is injected in container, take the basic fuchsin that parts by weight of component is 2.7 parts and the sodium thiosulfate that parts by weight of component is 16.7 parts is dissolved in wherein, it is subsequently adding the phosphoric acid that parts by weight of component is 8.3 parts, is sufficiently mixed 30 minutes, makes stock solution standby；
2, the stock solution of preparation in step 1 is injected in hermetic container, is simultaneously introduced the activated carbon decolorizing of 30g, at normal temperatures and pressures, seal lucifuge and place 36 hours, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
3, the solution obtained in step 2 is slowly added to citric acid solution, and by the pH regulator of solution to 2.15, makes reagent；
4, the reagent of gained in step 3 is encapsulated with 0.3ml dosage ampoule bottle or other similar containers nitrogen charging, be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
Exemplary application
The sample liquids of about 1ml is injected in the detectable of 0.2-0.6ml, mix 2-5 minute, if mixed liquor is colourless, then testing result display fluid to be measured is negative, and in sample liquids, the content of aldehyde is lower than 2ppm；If pinkish-purple occurs in mixing liquid, then may infer that in fluid to be measured, the content of aldehyde is more than 2ppm, and the color of mixing liquid is more deep, in the liquid of test sample, the concentration of aldehyde is more high。
The above is only the non-limiting embodiment of the present invention; for the person of ordinary skill of the art; under the premise conceived without departing from the invention and do not make creative work, it is also possible to making some deformation and improvement, these broadly fall into protection scope of the present invention。
Claims (2)
1. one kind is detected the reagent containing aldehyde in liquid, it is characterised in that: described reagent includes the component of following parts by weight of component:
Sodium thiosulfate is 14.4-16.7 part；Phosphoric acid is 7.2-8.3 part；Basic fuchsin is 2.1-2.7 part；Deionized water 972.2-976.4 part。
2. a kind of preparation method detecting the reagent containing aldehyde in liquid, it is characterised in that: described method carries out as follows:
(1), preparation stock solution: the deionized water that parts by weight of component is 972.2-976.4 part is injected in container, take the basic fuchsin that parts by weight of component is 2.1-2.7 part and the sodium thiosulfate that parts by weight of component is 14.4-16.7 part is dissolved in the deionized water that parts by weight of component is 972.2-976.4 part, it is subsequently adding the phosphoric acid that parts by weight of component is 7.2-8.3 part, it is sufficiently mixed 10-30 minute, makes stock solution standby；
(2), decolouring and standing and reacting: the stock solution of preparation in step (1) is injected in hermetic container, it is simultaneously introduced the activated carbon decolorizing of 20-30g, at normal temperatures and pressures, seals lucifuge and place 24-36 hour, carry out decolouring and standing and reacting, after having reacted, liquid is filtered；
(3), pH value is regulated: the solution obtained in step (2) is slowly added to citric acid solution, and by the pH regulator of solution to 1.95-2.15, makes reagent；
(4), prepare test kit: encapsulated with 0.1-0.3ml dosage ampoule bottle or similar containers nitrogen charging by the reagent of gained in step (3), be equipped with plastic suction pipe, connect liquid bag and colorimetric card makes Portable kit。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610058126.3A CN105699371A (en) | 2016-01-28 | 2016-01-28 | Reagent for detecting aldehyde contained in liquid and preparation method |
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| CN201610058126.3A CN105699371A (en) | 2016-01-28 | 2016-01-28 | Reagent for detecting aldehyde contained in liquid and preparation method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165301A (en) * | 1996-03-01 | 1997-11-19 | 美国拜尔公司 | Improved method for detection of protein |
| US6165797A (en) * | 1999-02-19 | 2000-12-26 | Bio-Defense Nutritionals, Inc. | Methods for testing oxidative stress |
| CN1672045A (en) * | 2002-07-30 | 2005-09-21 | 梅迪－泰克控股公司 | Agent for detecting malondialdehyde, method of making the same, and test kit for use thereof |
| KR20130043385A (en) * | 2011-10-20 | 2013-04-30 | 홍성창 | Reagent composition for the quantitation of active oxygen |
| CN103901029A (en) * | 2014-03-27 | 2014-07-02 | 华中科技大学 | Oxidative damage in-vitro detection method and detection kit |
| CN104024852A (en) * | 2012-01-31 | 2014-09-03 | Dfi株式会社 | Means for detecting oxygen free radicals in human body |
-
2016
- 2016-01-28 CN CN201610058126.3A patent/CN105699371A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165301A (en) * | 1996-03-01 | 1997-11-19 | 美国拜尔公司 | Improved method for detection of protein |
| US6165797A (en) * | 1999-02-19 | 2000-12-26 | Bio-Defense Nutritionals, Inc. | Methods for testing oxidative stress |
| CN1672045A (en) * | 2002-07-30 | 2005-09-21 | 梅迪－泰克控股公司 | Agent for detecting malondialdehyde, method of making the same, and test kit for use thereof |
| KR20130043385A (en) * | 2011-10-20 | 2013-04-30 | 홍성창 | Reagent composition for the quantitation of active oxygen |
| CN104024852A (en) * | 2012-01-31 | 2014-09-03 | Dfi株式会社 | Means for detecting oxygen free radicals in human body |
| CN103901029A (en) * | 2014-03-27 | 2014-07-02 | 华中科技大学 | Oxidative damage in-vitro detection method and detection kit |
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Application publication date: 20160622 |