CN105693040B - A kind of processing method of phosphorus-containing wastewater - Google Patents
A kind of processing method of phosphorus-containing wastewater Download PDFInfo
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- CN105693040B CN105693040B CN201610243184.3A CN201610243184A CN105693040B CN 105693040 B CN105693040 B CN 105693040B CN 201610243184 A CN201610243184 A CN 201610243184A CN 105693040 B CN105693040 B CN 105693040B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The present invention provides a kind of high-efficiency sewage processing method, including pretreatment, biochemical treatment, precipitation process, precipitation dirt concentration, thickened sludge dehydration.The method of the present invention all has preferable degradation capability, and the removal rate of phosphorus can reach more than 98%.Also have simultaneously go to denitrogenate well, the effect of ammonia, there is preferable commercial exploitation.
Description
Technical field
The present invention relates to a kind of sewage water treatment methods, belong to integrated wastewater processing technology field.
Background technology
Along with industrial development and the improvement of people's living standards, trade effluent amount and city domestic sewage
Amount just surges with surprising rapidity, these sewage or or is polluting river for the survival of mankind, river and lake, structure
One of the reason of environment for human survival being assisted into prestige.
Modern wastewater treatment method is broadly divided into physical treatment process, chemical logos and biological treatment three classes.At physics
Logos by physical action detaches, recycles the waste water for the pollutant (including oil film and oil droplet) in suspended state do not understood in waste water
Logos can be divided into gravitational separation process, centrifugal separation and sieving retention method etc..The processing unit for belonging to gravitational separation process has：It is heavy
It forms sediment, float (air supporting) etc., the processing equipment accordingly used is setting pot, sedimentation basin, oil separator, gas pond and its auxiliary equipment etc..
And existing various sewage water treatment methods, processing capacity are extremely limited, either sewage loads, still
Wastewater treatment efficiency all much can not meet the actual requirement of timely processing industry and sanitary sewage.Because at existing sewage
In reason method, such as biochemical process, aeration oxygen replenishing method, filtration method, not only investment is big, takes up a large area, and operating cost is high, and locates
Reason ability and treatment effect are undesirable；For another example simple Coagulation Method, then there is poor processing effects, and operating cost is high, and easily
Cause secondary pollution problems.In addition, existing sewage water treatment method, mostly there is the mating pipe network system of needs and such as
The problems such as what reuse water.Therefore, the task of top priority is to seek a kind of effective sewage water treatment method, increasingly tight to solve
The sewage discharge problem of weight.
Invention content
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of method of efficient sewerage processing and answer
With.
A kind of high-efficiency sewage processing method, it is characterised in that it includes following process steps：Pretreatment：Sewage was sent into
Filter tank removes bulky grain object and fiber impurity therein, is re-fed into setting pot, removes proportion in 2-3g/cm3, grain size is in 0.2mm
Above sand grains is sent into regulating reservoir；Biochemical treatment：Sewage is introduced into the anoxic pond in denitrification process, controls hydraulic retention
Time 10-15 hour, control dissolved oxygen are less than 0.5mg/L, remove BOD, while the nitrogen in NO3- is converted into nitrogen；By anoxic
The sewage that pond comes out introduces anaerobic pond, controls hydraulic detention time 10-15 hours, absorbs acetic acid, formic acid, ethyl alcohol etc. in sewage
Organic matter；The sewage that anaerobic pond comes out is introduced into the aerobic tank in nitrifying process, control hydraulic detention time 5-8 hours is adjusted
Whole pH value of sewage water 6.5-9 by the effect of polyP bacteria, makes the ammonium oxidation in sewage into nitrate, while by PO43 in sewage
In P excess sucking, so as to complete dephosphorization；Precipitation process：0.15-0.3Kg is added in sewage per ton to be prepared by following ratio
Into intermixture：Precipitating reagent: flocculant=8-9: 2-1, and the sewage for being mixed with intermixture is sent into clarifying basin, precipitation 2-3 is small
When, supernatant water up to standard and precipitation dirt are isolated, is arranged outside supernatant water；Precipitate dirt concentration：By the precipitation in clarifying basin
Dirt is sent into concentration basin, and flocculant, stirring concentration 10-15 hours, sludge after control concentration are added by the injected volume of 10-18mg/L
Moisture content is less than 97%；Thickened sludge dehydration：Thickened sludge is sent into blender, height is added by the input amount of 50mg/L
Molecular flocculant after stirring 0.3-0.5 hours, is sent into filter press, is pressed into the mud cake that moisture content is less than 75%.
The precipitating reagent is polysilicic acid aluminum chloride (iron), polysilicate sulfuric acid aluminium (iron), the diatom that diatom amount is 90-98%
One or more of soil, aluminium polychloride.
The flocculant is common inorganic salts and the high scores such as polyacrylamide, aluminium chloride, aluminum sulfate, ferric trichloride
One or more of subclass flocculant.
In sewage water treatment method of the present invention, the processing step of processing carries out successively in the following order：Pretreatment, it is raw
Change is handled, precipitation process, is precipitated dirt concentration, thickened sludge dehydration or is carried out successively in the following order：Pre- place
Reason, precipitation process, biochemical treatment precipitate dirt concentration, thickened sludge dehydration.
Wherein polyP bacteria is polyP bacteria commonly used in the art.Or the special efficacy disclosed in CN101386822A is gathered
Phosphorus bacterium, the bacterium are Pseudomonas fluorescens Pseudomonas fluorescens.) HJP07, preserving number is CGMCC NO
2164。
The present invention provides a kind of Qujjl-1 albumen to polyP bacteria with enhancing dephosphorization effect, the ammonia of the albumen simultaneously
Base acid sequence such as Genbank：Shown in WP_042484464.
Preferably, the polyP bacteria is the Pseudomonas fluorescens HJP07 for the gene for being transferred to coding Qujjl-1 albumen.
Meanwhile the present invention provides a series of Qujjl-1 protein variants, with stronger poly- phosphorus effect.By shown in
Variant amino acids when being expressed in recombinating polyP bacteria, it is found that it is poly- recombinant bacterium has relative to the polyP bacteria of the unconverted gene
Phosphorus effect improves at least more than 30% characteristic, has preferable effect.
The variant is in Qujjl-1 albumen (Genbank：WP_042484464 the basis of original amino acid)
On, following mutation 24D/V, 60Q/F, 122Q/G, 153E/S, 183N/G, 199I/A, 219N/S, 236A/G are carried out respectively,
253P/A, 309F/G, 367S/P, 373D/G.(24D/V represents that the D amino acid substitutions in original series the 24th are V amino
Acid).
Preferably, polyP bacteria of the invention for be transferred to any one following Qujjl-1 protein variant of coding (relative to
Qujjl-1 albumen original amino acid carries out being mutated 24D/V, 60Q/F, 122Q/G, 153E/S, 183N/G as follows respectively,
199I/A, 219N/S, 236A/G, 253P/A, 309F/G, 367S/P or 373D/G) gene Pseudomonas fluorescens HJP07.
Simultaneously the present invention provides a kind of preparation method of Qujjl-1 genes, by polyP bacteria under high concentration phosphorus and environment
72h is cultivated respectively under without phosphorus environment.It centrifuges, collection thalline, ultrasonic disruption under cryogenic conditions adds in DTT, then purifies egg
In vain, bidirectional two-dimensional electrophoresis is carried out, by the difference of protein expression profiles before and after analysis domestication, searches out 5, the albumen of differential expression,
It is sequenced by mass spectrum, obtains corresponding amino acid sequence.It is compared by blast, obtains its corresponding nucleotide sequence.It is logical
It crosses in the channel genes to expression bacterium, is tested by poly- phosphorus, it was found that Qujjl-1 genes have preferable poly- phosphorus reaction.
Simultaneously the present invention provides a kind of polyP bacteria for having imported Qujjl-1 genes, using DNAMAN software Design primers,
BamHI and SalI restriction enzyme sites have been separately added into, amplification has been carried out and obtains target fragment, it is (logical that PCR amplification obtains Qujjl-1 genes
Cross multiplex PCR to introduce corresponding mutational site in the gene order of its coding, so as to obtain different mutants gene),
Double digestion PCR product is carried out with BamHI and SalI, the cloning vector with also passing through BamHI and SalI double digestions
PWB980 is connected, and the recombinant plasmid transformed being proved to be successful is entered in polyP bacteria, obtains the genetic engineering bacterium of degradation phosphorus.
A kind of application of genetic engineering bacterium for phosphorus of degrading in phosphorus of degrading.
The advantages and positive effects of the present invention are：
I, the present invention provides a kind of method of efficient sewage disposal, and one is cloned into from from polyP bacteria genome
Gene, by DNA recombinant techniques, clonal expression builds the polyP bacteria recombinated in vitro, and the Yi Walk that go forward side by side verify that this recombinant bacterium drops
Phosphorus characteristic obtains the genetically engineered bacteria for having stronger degradation capability to phosphorus, this will provide the industrialized production of phosphorus degradation bacteria
New theoretical foundation.
2nd, the recombinant bacterium that the present invention is built all has the phosphorus of various concentration preferable degradation capability, and the removal rate of phosphorus can reach
To more than 98%.Also have simultaneously go to denitrogenate well, the effect of ammonia, there is preferable commercial exploitation.
Specific embodiment
Zuo Jin ー of the present invention are walked with reference to specific embodiment and are described in detail, following embodiment is descriptive, is not limit
Qualitatively, it is impossible to which protection scope of the present invention is limited with this.
The method of 1 sewage disposal of embodiment
A kind of high-efficiency sewage processing method, it is characterised in that it includes following process steps：
Handled sewage quality is as follows：CODcr450mg/L, BOD5150mg/L, SS≤200mg/L, NH3-N≤
130mg/L, T-P≤100mg/L, pH=6.5-9.0.
(1) it pre-processes：Sewage is sent into filtering ponds, wherein original phosphorus content is miscellaneous to remove bulky grain object and fiber therein
Matter, is re-fed into setting pot, removes proportion in 2-3g/cm3, and grain size is sent into regulating reservoir in the sand grains of more than 0.2mm；
(2) biochemical treatment：Sewage is introduced into the anoxic pond in denitrification process, control hydraulic detention time 8-12 is small
When, control dissolved oxygen is less than 0.6mg/L, removes BOD, while the nitrogen in NO3- is converted into nitrogen；The dirt that anoxic pond is come out
Water introduces anaerobic pond, controls hydraulic detention time 16-17 hours, the organic matters such as acetic acid, formic acid, ethyl alcohol in absorption sewage；It will
The sewage that anaerobic pond comes out is introduced into the aerobic tank in nitrifying process, and control hydraulic detention time 4-5 hours adjusts pH value of sewage water
6.5-9 by the effect of Pseudomonas fluorescens HJP07, makes the ammonium oxidation in sewage into nitrate, while will be in sewage
P excess sucking in PO43-, so as to complete dephosphorization；
(3) precipitation process：The intermixture that 0.15-0.3Kg is configured to by following ratio is added in sewage per ton：Precipitating reagent
: flocculant=8-9: 2-1, and the sewage that will be mixed with intermixture is sent into clarifying basin, is precipitated 2-3 hours, is isolated up to standard upper
Clear water and precipitation dirt, supernatant water are arranged outside；
(3) dirt concentration is precipitated：Precipitation dirt in clarifying basin is sent into concentration basin, by the dispensing of 10-18mg/L
Amount adds flocculant, and stirring concentration 10-15 hours, moisture percentage in sewage sludge is less than 97% after control concentration；
(4) thickened sludge dehydration：Thickened sludge is sent into blender, macromolecule is added by the input amount of 50mg/L
Flocculant after stirring 0.3-0.5 hours, is sent into filter press, is pressed into the mud cake that moisture content is less than 75%.
The precipitating reagent is polysilicic acid aluminum chloride (iron), diatom amount is 90% diatomite, the flocculant are polypropylene
Amide.
After processing, sewage quality CODcr50mg/L, BOD501mg/L, SS=10mg/L, NH3-N=20mg/L, T-P
=10mg/L, pH=9.
The structure of 2 polyP bacteria genetic engineering bacterium of embodiment
According to DNAMAN software Design primers are utilized, it is separately added into BamHI and SalI restriction enzyme sites, forward primer sequence
For：ATGTCGCAACTCAATTCCAA；Reverse primer sequences are：GGAGTGCAGCTGTACGGGGTC.
It carries out amplification and obtains target fragment, PCR amplification obtains Qujjl-1 genes, and clip size is；1200bp or so,
By sequencing, it is found that amplification is correct.
By multiplex PCR by corresponding 24D/V, 60Q/F, 122Q/G, 153E/S, 183N/G, 199I/A, 236A/G,
253P/A, 309F/G, 367S/P, 373D/G mutational site are introduced between in the gene order of its coding and (can refer to existing skill
The preparation method of art can obtain).It so as to obtain different mutants gene, is loaded into expression vector PWB980, will test
It demonstrate,proves successful recombinant plasmid transformed to enter in polyP bacteria, obtains the genetic engineering bacterium of degradation phosphorus.
The dephosphorization compliance test result of 3 polyP bacteria genetic engineering bacterium of embodiment
According to the method for embodiment 1, corresponding sewage disposal experiment is carried out, different recombinant bacteriums is respectively adopted and carries out poly- phosphorus
Reaction, is found through experiments that, 24D/V, 60Q/F, 122Q/G, 153E/S, 183N/G, 199I/A, 236A/G, 253P/A, 309F/
G, 367S/P, 373D/G these types mutant bacteria all with relative to original bacteria with the effect being remarkably reinforced, and 219N/S bacterium
Show the poly- phosphorus effect not enhanced.Total processing time is not more than 3 days, and result is as follows：Sewage is same a collection of sewage, so as to
Guaranteed conditions are consistent.
It can be seen that using the present processes, the efficiency of 3d dephosphorizations than the prior art will be high.It therefore, can be with
For large-scale sewage disposal.And also there is preferable effect in the removal of nitrogen and ammonia, by detection, find substantially
There is no the residuals of ammonia and nitrogen.
Tested simultaneously using the TP of 80,120,160 concentration, can also equally achieve the effect that it is identical, it is and existing
The method of technology all shows the removal ability of reduction in the Tp of high concentration.Therefore, method of the invention has stronger
Adaptability.
Claims (4)
1. a kind of high-efficiency sewage processing method, including following process steps：Pretreatment：Sewage is sent into filtering ponds, removal is wherein
Bulky grain object and fiber impurity, be re-fed into setting pot, remove proportion in 2-3g/cm3, grain size sent in the sand grains of more than 0.2mm
Enter regulating reservoir；Biochemical treatment：Sewage is introduced into the anoxic pond in denitrification process, control hydraulic detention time 10-15 hours,
Dissolved oxygen is controlled to be less than 0.5mg/L, removes BOD, while by NO3 -In nitrogen be converted into nitrogen；The sewage that anoxic pond comes out is drawn
Enter anaerobic pond, control hydraulic detention time 10-15 hours absorbs acetic acid, formic acid, the ethyl alcohol in sewage；Anaerobic pond is come out
Sewage is introduced into the aerobic tank in nitrifying process, and control hydraulic detention time 5-8 hours adjusts pH value of sewage water 6.5-9, by poly-
The effect of phosphorus bacterium makes the ammonium oxidation in sewage into nitrate, while by PO in sewage4 3- P excess sucks, so as to complete dephosphorization；
Precipitation process：The intermixture that 0.15-0.3Kg is configured to by following ratio is added in sewage per ton：Precipitating reagent: flocculant=8-
9: 2-1, and the sewage for being mixed with intermixture is sent into clarifying basin, it precipitates 2-3 hours, isolates supernatant water up to standard and precipitation is dirty
Object, supernatant water are arranged outside；Precipitate dirt concentration：Precipitation dirt in clarifying basin is sent into concentration basin, by the throwing of 10-18mg/L
Flocculant is high-volume added, stirring concentration 10-15 hours, moisture percentage in sewage sludge is less than 97% after control concentration；At thickened sludge dehydration
Reason：Thickened sludge is sent into blender, high polymer coagulant is added by the input amount of 50mg/L, after stirring 0.3-0.5 hours,
Filter press is sent into, is pressed into the mud cake that moisture content is less than 75%；It is characterized in that：The polyP bacteria is has been transferred to Qujjl-1
The albumen of Pseudomonas fluorescens HJP07, Qujjl-1 the protein variant gene code of protein variant gene is relative to Qujjl-1 eggs
For white original amino acid there are the variation of 253P/A, Qujjl-1 albumen original amino acid is Genbank：WP_
042484464。
2. the method as described in claim 1, it is characterised in that：The precipitating reagent for polysilicic acid aluminum chloride, polysilicate sulfuric acid aluminium,
Diatom amount is one or more of the diatomite of 90-98%, aluminium polychloride.
3. the method as described in claim 1, it is characterised in that：The flocculant is polyacrylamide, aluminium chloride, sulfuric acid
One or more of aluminium, ferric trichloride.
4.Qujjl-1 protein variants, it is characterised in that：It is mutated as follows relative to Qujjl-1 albumen original amino acid
253P/A, Qujjl-1 albumen original amino acid referring to：Genbank：WP_042484464.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1669956A (en) * | 2004-03-18 | 2005-09-21 | 昆明柯利欣环保科技有限公司 | High efficiency sewage treatment method |
| CN103992013A (en) * | 2014-06-09 | 2014-08-20 | 江苏德鑫环保科技有限公司 | Method and equipment for treating high-concentration refractory chemical wastewater |
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2015
- 2015-05-20 CN CN201610243184.3A patent/CN105693040B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1669956A (en) * | 2004-03-18 | 2005-09-21 | 昆明柯利欣环保科技有限公司 | High efficiency sewage treatment method |
| CN103992013A (en) * | 2014-06-09 | 2014-08-20 | 江苏德鑫环保科技有限公司 | Method and equipment for treating high-concentration refractory chemical wastewater |
Non-Patent Citations (1)
| Title |
|---|
| WP_042484464;genbank;《genbank》;20150109;第1页 * |
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Effective date of registration: 20180523 Address after: 528012 Zhongyi Road, Chancheng District, Foshan, Guangdong Province, No. 58 Applicant after: Foshan new source sewage treatment Co., Ltd. Address before: 330022 Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi Applicant before: Yu Suping |
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