CN105688269B - Modified gelatin hemostatic material and preparation method thereof - Google Patents
Modified gelatin hemostatic material and preparation method thereof Download PDFInfo
- Publication number
- CN105688269B CN105688269B CN201610075837.1A CN201610075837A CN105688269B CN 105688269 B CN105688269 B CN 105688269B CN 201610075837 A CN201610075837 A CN 201610075837A CN 105688269 B CN105688269 B CN 105688269B
- Authority
- CN
- China
- Prior art keywords
- gelatin
- hemostatic material
- modified gelatin
- modified
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/104—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a modified gelatin hemostatic material and a preparation method thereof. The preparation method of the modified gelatin hemostatic material comprises the following steps: preparing gelatin into a gelatin solution, adding an aldehyde cross-linking agent with the content of 0.001-0.05% relative to the gelatin, treating for 15-45 min under the microwave with the power of 100-600W, and then cleaning and drying. The gelatin hemostatic material prepared by the method has small swelling rate and can be used for treating narrow wound surfaces; and the content of the aldehyde crosslinking agent is low, and the gelatin has better safety compared with the existing gelatin product.
Description
Technical Field
The invention relates to the field of medical treatment, in particular to a modified gelatin material with a hemostatic effect.
Background
The gelatin has good biocompatibility, biodegradability and bioactivity, and has wide application in the field of biological medical treatment. However, uncrosslinked gelatin is readily soluble in water and easily degradable in vivo, and its dried product has a brittle texture, poor mechanical strength and high swelling degree. Therefore, in practical applications, crosslinking modification is often performed by a certain method to meet the performance requirements of the applications.
Bleeding is the most common condition encountered in surgical operations, and the existing hemostatic products are mainly in block or bulk shapes, such as sponges, gauze, dressings, and the like, which are not suitable for uneven wounds or narrow wounds, because the hemostatic products cannot form a good fit with the wound or cannot reach the surface of the wound at all. There is therefore a need for a hemostatic material that is capable of flowing. Although the existing powdery gelatin hemostatic materials are hydrated and then extruded by an injector, the hemostatic materials have good applicability to general uneven and/or narrow wound surfaces. However, the swelling rate of the existing gelatin hemostatic materials is high, and many nerves exist in some narrow parts, such as tumor cavities, nasal cavities, dura mater, spinal venous plexus and the like, and after the gelatin is swelled, the nerves are easily compressed, so that patients feel uncomfortable, and even serious consequences can be caused.
Therefore, the development of a hemostatic material with a small swelling ratio is urgently needed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a modified gelatin hemostatic material. The modified gelatin hemostatic material has a small swelling ratio and is suitable for treating narrow wounds.
The invention also aims to provide a preparation method of the modified gelatin hemostatic material.
The invention also aims to provide application of the modified gelatin hemostatic material.
The invention achieves the above object by the following technical scheme.
A preparation method of a modified gelatin hemostatic material comprises the following steps: preparing gelatin into a gelatin solution, adding an aldehyde cross-linking agent accounting for 0.001-0.05% of the mass of the gelatin, treating for 15-45 min under the microwave with the power of 100-600W, and then cleaning and drying.
The invention can obtain the modified gelatin hemostatic material with low swelling ratio under the condition of extremely low content of the cross-linking agent through the action of the microwave, so that the hemostatic material has the characteristics of small toxic and side effect and low swelling ratio, and can be suitable for treating narrow wounds.
Preferably, the modified gelatin hemostatic material is in a powder or granule form.
Preferably, the swelling ratio of the modified gelatin hemostatic material is 5-10%.
Preferably, the weight percentage concentration of the gelatin in the gelatin solution is 1-20%.
Preferably, the aldehyde crosslinker is glutaraldehyde and/or formaldehyde.
Preferably, the power of the microwave is 400-600W.
Preferably, the time of the microwave treatment is 15-30 min.
Preferably, the solvent of the gelatin solution is purified water, physiological saline, water for injection or PBS neutral solution.
The preparation method of the modified gelatin hemostatic material comprises the following steps:
s1, dissolving gelatin in a solvent to prepare a gelatin solution;
s2, adding a cross-linking agent into the gelatin solution;
s3, treating the solution with microwave with power of 100-600W for 15-45 min;
s4, filtering, and removing the solvent to obtain modified gelatin;
and S5, cleaning the modified gelatin, removing the unreacted cross-linking agent, and drying to obtain the gelatin hemostatic material.
In the present invention, the drying may be carried out by a conventional drying method such as vacuum drying or freeze drying.
Preferably, the step of drying comprises:
s6, placing the cleaned modified gelatin in a refrigerator at the temperature of-20-0 ℃ or in liquid nitrogen for prefreezing to solidify the gelatin;
s7, freeze drying to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery or granular gelatin hemostatic material.
Preferably, in S5, the washing modified gelatin is obtained by washing the modified gelatin with water, ethanol or methanol or a mixture of ethanol and methanol and water in sequence until the pH value is neutral.
Preferably, the barrier used in the filtration in s4 is a nonwoven fabric or a filter membrane with dense gaps. The barrier only allows liquid solvent to pass through but not gelatin particles.
The invention also discloses application of the modified gelatin hemostatic material in preparation of wound treatment materials. The use method of the modified gelatin hemostatic material comprises the following steps: the modified gelatin hemostatic material is weighed and added with a certain amount of normal saline to quickly form uniform sticky matter which has fluidity and can be injected to the wound surface through an injector to achieve the hemostatic effect.
Compared with the prior art, the invention has the following beneficial effects:
the modified gelatin hemostatic material prepared by the invention has a small swelling ratio of only 5-10%, and can meet the requirements of narrow wound treatment; by the microwave treatment technology, the cross-linking agent with the dosage far lower than that of the prior art can be adopted to obtain the modified gelatin hemostatic material with low swelling ratio, and the content of the cross-linking agent is low, so that the toxicity is lower than that of the prior gelatin product, and the safety is improved; the prepared modified gelatin hemostatic material can be dissolved in normal saline to quickly form uniform sticky substances, has good fluidity, can be injected by an injector, and is suitable for being used as a material for wound treatment.
Detailed Description
The present invention is described below with reference to specific examples, however, the scope of the present invention is not limited to the following examples, and the descriptions of the examples are only used to help understanding the method of the present invention and the core idea thereof. Any modification, equivalent replacement, and improvement made within the spirit and principle of this patent shall be included in the protection scope of the present claims.
Example 1
S1, dissolving 10g of gelatin in 90g of pure water to prepare a gelatin solution with the concentration of 10%, and suspending for 1h to fully swell the gelatin;
s2, adding a glutaraldehyde crosslinking agent, wherein the addition amount of glutaraldehyde accounts for 0.01% of the mass of gelatin;
s3, treating the solution with microwave with power of 150W for 20 min;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 3 times, washing with ethanol for 3 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, pre-freezing the cleaned modified gelatin in a refrigerator at the temperature of-20 ℃ for 20 hours to solidify the modified gelatin;
s7, drying for 24 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery gelatin hemostatic material.
Example 2
S1, dissolving 5g of gelatin in 95g of pure water to prepare a gelatin solution with the concentration of 5%, and suspending for 1h to fully swell the gelatin;
s2, adding a glutaraldehyde crosslinking agent, wherein the addition amount of glutaraldehyde accounts for 0.05% of the mass of gelatin;
s3, treating the solution with microwave with power of 400W for 15 min;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 4 times, washing with ethanol for 2 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, placing the cleaned modified gelatin in liquid nitrogen for prefreezing to solidify the gelatin, filtering the massive frozen object, grinding, and adding liquid nitrogen to prevent the frozen object from melting;
s7, drying for 24 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the granular gelatin hemostatic material.
Example 3
S1, dissolving 10g of gelatin in 90g of pure water to prepare a gelatin solution with the concentration of 10%, and suspending for 1h to fully swell the gelatin;
s2, adding a glutaraldehyde crosslinking agent, wherein the addition amount of glutaraldehyde accounts for 0.001% of the mass of gelatin;
s3, treating the solution with microwave with power of 600W for 30 min;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 5 times, washing with ethanol for 3 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, pre-freezing the cleaned modified gelatin in a refrigerator at the temperature of-20 ℃ for 4 hours to solidify the modified gelatin;
s7, drying for 48 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery gelatin hemostatic material.
Example 4
S1, dissolving 8g of gelatin in 92g of pure water to prepare a gelatin solution with the concentration of 8%, and suspending for 1h to fully swell the gelatin;
s2, adding a formaldehyde cross-linking agent, wherein the adding amount of formaldehyde accounts for 0.007% of the mass of the gelatin;
s3, treating the solution with microwave with power of 500W for 15 min;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 3 times, washing with ethanol for 3 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, pre-freezing the cleaned modified gelatin in a refrigerator at the temperature of-20 ℃ for 20 hours to solidify the modified gelatin;
s7, drying for 24 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery gelatin hemostatic material.
Comparative example 1
S1, dissolving 10g of gelatin in 90g of pure water to prepare a gelatin solution with the concentration of 10%, and suspending for 1h to fully swell the gelatin;
s2, adding a glutaraldehyde crosslinking agent, wherein the addition amount of glutaraldehyde accounts for 0.001% of the mass of gelatin;
s3, stirring for 72 hours;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 5 times, washing with ethanol for 3 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, pre-freezing the cleaned modified gelatin in a refrigerator at the temperature of-20 ℃ for 4 hours to solidify the modified gelatin;
s7, drying for 48 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery gelatin hemostatic material.
Comparative example 2
S1, dissolving 10g of gelatin in 90g of pure water to prepare a gelatin solution with the concentration of 10%, and suspending for 1h to fully swell the gelatin;
s2, adding a glutaraldehyde crosslinking agent, wherein the addition amount of glutaraldehyde accounts for 0.01% of the mass of gelatin;
s3, treating the solution with microwave with power of 700W for 20 min;
s4, filtering by using non-woven fabric, and removing the solvent to obtain modified gelatin;
s5, washing with clear water for 3 times, washing with ethanol for 3 times, washing the modified gelatin with clear water, and removing unreacted cross-linking agent;
s6, pre-freezing the cleaned modified gelatin in a refrigerator at the temperature of-20 ℃ for 20 hours to solidify the modified gelatin;
s7, drying for 24 hours by using a low-temperature low-pressure freeze dryer to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery gelatin hemostatic material.
Swelling ratio measurement
The swelling ratio was determined by the following method: taking 1g of the modified gelatin hemostatic material prepared by the method of the invention, soaking the material in 100g of PBS buffer solution with the pH value of 7.4 +/-0.2, standing at room temperature for 24h, filtering out gelatin, wiping off residual water by using filter paper, and weighing. According to the following formula:
swelling ratio = (sample wet weight-sample dry weight)/sample dry weight 100%
And calculating the swelling ratio of the gelatin.
The swelling ratio test results of the modified gelatin hemostatic materials of examples 1-4 and comparative examples 1-2 are shown in table 1. As can be seen from the comparison of example 3 with comparative example 1, the swelling ratio of the modified gelatin subjected to microwave treatment was significantly reduced; according to the comparison between example 1 and comparative example 2, too high microwave power results in an increase in the swelling ratio of the modified gelatin; the swelling ratio of the modified gelatin is obviously reduced compared with that of the unmodified gelatin.
TABLE 1 swelling Rate test results for the products of examples and comparative examples
| Test object | Swelling ratio (%) |
| Practice ofExample 1 | 7.5 |
| Example 2 | 5.6 |
| Example 3 | 9.3 |
| Example 4 | 6.8 |
| Comparative example 1 | 95.1 |
| Comparative example 2 | 80.7 |
| Gelatin before modification | 150~300 |
Biosafety testing
In order to examine the safety of the modified gelatin of the present invention, a skin irritation test, a cytotoxicity test and a sensitization test were performed by the following methods:
(1) preparing a leaching solution: according to GB/T16886.12-2005, taking the modified gelatin hemostatic material to prepare leaching liquor;
(2) skin irritation test: according to GB/T16886.10-2005, 0.2mL of the above leaching solution was injected into skin, and edema and red swelling of the tested part were observed at 2h, 24h and 72h after injection.
(3) Cytotoxicity test: the samples were tested for cytotoxicity of the modified gelatin hemostatic material according to the agar overlay method specified in GB/T16886.5.
(4) Sensitization test: taking the prepared leaching liquor, and determining the sensitization of the modified gelatin hemostatic material on the tested animal according to the method specified in GB/T16886.10-2005.
According to the above detection method, the modified gelatin hemostatic materials of examples 1 to 4 were used for a skin irritation test, a cytotoxicity test, and a sensitization test, respectively. As a result, the skin irritation reaction and cytotoxicity of the products of examples 1-4 are all 0-1 grade, and no sensitization is shown, which indicates that the modified gelatin hemostatic material has good safety and biocompatibility.
Animal experiments
The hemostasis performance test method of the invention comprises the following steps: anaesthetizing the rabbit, and then puncturing the artery blood vessel with No. 16 needle, thereby generating a wound in the ear artery of the rabbit; the modified gelatin hemostatic materials prepared in the embodiments 1-4 are respectively weighed, then a certain amount of normal saline is added to form a uniform viscous substance, the viscous substance is injected on the wounds of the ears of rabbits through an injector to cover the wounds, and the hemostatic materials are removed from the bleeding areas after 3 minutes. The wounds treated with the samples of examples 1-4 were observed for the absence of reoccurrence of bleeding.
As a result, no bleeding occurred again in any of the wounds treated with the modified gelatin hemostatic materials of examples 1-4. The product of the invention has good hemostatic performance.
Claims (11)
1. The preparation method of the modified gelatin hemostatic material is characterized by comprising the following steps: preparing gelatin into a gelatin solution, adding an aldehyde cross-linking agent accounting for 0.001-0.05% of the mass of the gelatin, treating for 15-45 min under the microwave with the power of 100-600W, and then cleaning and drying.
2. The modified gelatin hemostatic material of claim 1, wherein the modified gelatin hemostatic material is in a powder or granular form.
3. The modified gelatin hemostatic material of claim 1, wherein the swelling ratio of the modified gelatin hemostatic material is 5% to 10%.
4. The modified gelatin hemostatic material of claim 1, wherein the gelatin solution comprises 1-20% gelatin by weight.
5. The modified gelatin hemostatic material of claim 1, wherein the aldehyde cross-linking agent is glutaraldehyde and/or formaldehyde.
6. The modified gelatin hemostatic material of claim 1, wherein the microwave has a power of 400-600W.
7. The modified gelatin hemostatic material of claim 1, wherein the solvent of the gelatin solution is purified water, normal saline, water for injection or PBS neutral solution.
8. The method for preparing the modified gelatin hemostatic material as claimed in any one of claims 1 to 7, comprising the steps of:
s1, dissolving gelatin in a solvent to prepare a gelatin solution;
s2, adding a cross-linking agent into the gelatin solution;
s3, treating the solution with microwave with power of 100-600W for 15-45 min;
s4, filtering, and removing the solvent to obtain modified gelatin;
and S5, cleaning the modified gelatin, removing the unreacted cross-linking agent, and drying to obtain the gelatin hemostatic material.
9. The method for preparing a modified gelatin hemostatic material of claim 7, wherein the drying step comprises:
s6, placing the cleaned modified gelatin in a refrigerator at the temperature of-20-0 ℃ or in liquid nitrogen for prefreezing to solidify the gelatin;
s7, freeze drying to obtain flaky modified gelatin;
and S8, grinding or crushing to obtain the powdery or granular gelatin hemostatic material.
10. The method for preparing the modified gelatin hemostatic material according to claim 7, wherein in S5, the cleaning modified gelatin is water, ethanol or methanol or a mixture of ethanol and methanol, and the water cleaning modified gelatin is sequentially performed until the pH value is neutral.
11. The use of the modified gelatin hemostatic material of any one of claims 1-7 in the preparation of a wound healing material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075837.1A CN105688269B (en) | 2016-02-03 | 2016-02-03 | Modified gelatin hemostatic material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075837.1A CN105688269B (en) | 2016-02-03 | 2016-02-03 | Modified gelatin hemostatic material and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105688269A CN105688269A (en) | 2016-06-22 |
| CN105688269B true CN105688269B (en) | 2021-04-16 |
Family
ID=56230153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610075837.1A Active CN105688269B (en) | 2016-02-03 | 2016-02-03 | Modified gelatin hemostatic material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105688269B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201932124A (en) * | 2018-01-18 | 2019-08-16 | 近鎰生技股份有限公司 | Hemostatic material, preparation method thereof and pharmaceutical composition containing the same |
| CN108187129B (en) * | 2018-02-07 | 2021-08-31 | 广州迈普再生医学科技股份有限公司 | Absorbable gelatin styptic powder and preparation method thereof |
| CN108187128B (en) * | 2018-02-07 | 2021-08-31 | 广州迈普再生医学科技股份有限公司 | Absorbable hemostatic bone wax and preparation method thereof |
| CN108310448B (en) * | 2018-02-07 | 2021-03-02 | 广州迈普再生医学科技股份有限公司 | Preparation method of fluid gelatin hemostatic material |
| CN108394199A (en) * | 2018-05-07 | 2018-08-14 | 安徽索亚装饰材料有限公司 | A kind of preparation method of the without phosphorus fountain solution of surface-active |
| CN108815563A (en) * | 2018-07-23 | 2018-11-16 | 天津市长江医疗器械有限公司 | A kind of gelatin hemostatic material and its preparation process |
| CN110179514A (en) * | 2019-06-13 | 2019-08-30 | 南昌大学第二附属医院 | The preparation method of gelfoam suspension applied to neurosurgery hemostasis |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283454A (en) * | 1999-08-05 | 2001-02-14 | 弗·哈夫曼-拉罗切有限公司 | Method for preparing pellet contg fat-soluble substances |
| CN1921896A (en) * | 2004-01-30 | 2007-02-28 | 弗罗桑公司 | Haemostatic sprays and compositions |
| CN101854960A (en) * | 2006-12-15 | 2010-10-06 | 生命连结有限公司 | Gelatin-transglutaminase hemostatic dressings and sealants |
| WO2014028209A1 (en) * | 2012-08-14 | 2014-02-20 | The Trustees Of The University Of Pennsylvania | Stabilizing shear-thinning hydrogels |
| CN104127909A (en) * | 2014-08-19 | 2014-11-05 | 张秀华 | Gelatin sponge for trauma hemostasis, as well as preparation method of gelatin sponge |
-
2016
- 2016-02-03 CN CN201610075837.1A patent/CN105688269B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1283454A (en) * | 1999-08-05 | 2001-02-14 | 弗·哈夫曼-拉罗切有限公司 | Method for preparing pellet contg fat-soluble substances |
| CN1921896A (en) * | 2004-01-30 | 2007-02-28 | 弗罗桑公司 | Haemostatic sprays and compositions |
| CN101854960A (en) * | 2006-12-15 | 2010-10-06 | 生命连结有限公司 | Gelatin-transglutaminase hemostatic dressings and sealants |
| WO2014028209A1 (en) * | 2012-08-14 | 2014-02-20 | The Trustees Of The University Of Pennsylvania | Stabilizing shear-thinning hydrogels |
| CN104127909A (en) * | 2014-08-19 | 2014-11-05 | 张秀华 | Gelatin sponge for trauma hemostasis, as well as preparation method of gelatin sponge |
Non-Patent Citations (2)
| Title |
|---|
| 强力止血粉在兽医临床上的应用;周振江等;《 哈尔滨商业大学学报(自然科学版)》;19800131(第1期);56-57 * |
| 微波交联CTS/GLT共混膜的制备及性能研究;胡思前等;《现代塑料加工应用》;20070331;第19卷(第3期);17-19 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105688269A (en) | 2016-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105688269B (en) | Modified gelatin hemostatic material and preparation method thereof | |
| Wu et al. | Anti-oxidant anti-inflammatory and antibacterial tannin-crosslinked citrate-based mussel-inspired bioadhesives facilitate scarless wound healing | |
| US11130823B2 (en) | Method for producing hydrogels | |
| AU2006329660B2 (en) | Hemostatic material | |
| KR20230125136A (en) | Wound dressing comprising hyaluronic acid-calcium and polylysine, and preparation method thereof | |
| Chen et al. | A triple-network carboxymethyl chitosan-based hydrogel for hemostasis of incompressible bleeding on wet wound surfaces | |
| DE19851334A1 (en) | Flexible fibrin-based wound dressing and process for its manufacture | |
| Mirzadeh et al. | Preparation of chitosan derived from shrimp | |
| JP2023118919A (en) | Hemostatic material | |
| CN105521520B (en) | Preparation method of silkworm fibroin hemostatic material | |
| Ma et al. | Oxidized dextran crosslinked polysaccharide/protein/polydopamine composite cryogels with multiple hemostatic efficacies for noncompressible hemorrhage and wound healing | |
| CN105251036A (en) | Medical hemostatic material and preparation method thereof | |
| US2764159A (en) | Absorbable products | |
| WO2019026177A1 (en) | Hemostatic material and wound dressing material containing same | |
| CN115607727B (en) | Photo-thermal multifunctional hydrogel based on bletilla polysaccharide, preparation method and application thereof, and medicine for wound repair | |
| Ma et al. | A dual network cross-linked hydrogel with multifunctional Bletilla striata polysaccharide/gelatin/tea polyphenol for wound healing promotion | |
| CN114028604B (en) | Multi-component wound repair hemostasis dressing based on polymerized amino acid and application thereof | |
| CN113750287B (en) | Water-soluble hemostatic sponge and preparation method thereof | |
| KR20160038120A (en) | Alginate hydrogel and manufacturing method thereof | |
| Ma et al. | Collagen Scaffolds Functionalized by Cu2+‐Chelated EGCG Nanoparticles with Anti‐Inflammatory, Anti‐Oxidation, Vascularization, and Anti‐Bacterial Activities for Accelerating Wound Healing | |
| CN114230808A (en) | Preparation method of antibacterial gel with amino polyethylene glycol as gel matrix | |
| CA1266767A (en) | Absorbent polymer material and its preparation | |
| EP3148599A1 (en) | Method for preparing neutralized matrix of non-antigenic collagenous material | |
| EP4306138A1 (en) | Hemostatic material | |
| CN115197866B (en) | Paenibacillus, hemostatic polysaccharide produced by same and application of hemostatic polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 510663 Guangdong Guangzhou high tech Industrial Development Zone, Science City, 80 E third district. Applicant after: Guangzhou Maple regenerative medicine Polytron Technologies Inc Address before: 510663 Guangzhou District, Guangdong, new high tech Industrial Development Zone, Science City, 80 E third district. Applicant before: Medprin Regenerative Medical Technologies Co., Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |