CN105688253A - Method for in vivo wound surface anoxic treatment - Google Patents
Method for in vivo wound surface anoxic treatment Download PDFInfo
- Publication number
- CN105688253A CN105688253A CN201610044233.0A CN201610044233A CN105688253A CN 105688253 A CN105688253 A CN 105688253A CN 201610044233 A CN201610044233 A CN 201610044233A CN 105688253 A CN105688253 A CN 105688253A
- Authority
- CN
- China
- Prior art keywords
- wound surface
- polyvinylidene chloride
- film
- chloride film
- anoxic treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000001727 in vivo Methods 0.000 title abstract 3
- 229920001328 Polyvinylidene chloride Polymers 0.000 claims abstract description 55
- 239000005033 polyvinylidene chloride Substances 0.000 claims abstract description 55
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 4
- 239000012535 impurity Substances 0.000 claims description 3
- 235000014593 oils and fats Nutrition 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 2
- 206010021143 Hypoxia Diseases 0.000 abstract description 16
- 206010002660 Anoxia Diseases 0.000 abstract description 9
- 241000976983 Anoxia Species 0.000 abstract description 9
- 230000007953 anoxia Effects 0.000 abstract description 9
- 230000035876 healing Effects 0.000 abstract description 7
- 239000012528 membrane Substances 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 230000002950 deficient Effects 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 74
- 208000027418 Wounds and injury Diseases 0.000 description 74
- 239000011116 polymethylpentene Substances 0.000 description 24
- 229920000306 polymethylpentene Polymers 0.000 description 24
- 210000003491 skin Anatomy 0.000 description 24
- 241000699670 Mus sp. Species 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 230000037314 wound repair Effects 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 230000006399 behavior Effects 0.000 description 4
- 230000003013 cytotoxicity Effects 0.000 description 4
- 231100000135 cytotoxicity Toxicity 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 4
- 230000002500 effect on skin Effects 0.000 description 4
- 230000001146 hypoxic effect Effects 0.000 description 4
- 210000002510 keratinocyte Anatomy 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000010261 cell growth Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000004209 hair Anatomy 0.000 description 3
- 230000007954 hypoxia Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000029663 wound healing Effects 0.000 description 3
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 240000001624 Espostoa lanata Species 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 2
- 206010048222 Xerosis Diseases 0.000 description 2
- 230000012292 cell migration Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000001447 compensatory effect Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 230000002951 depilatory effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 210000003470 mitochondria Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical group ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 1
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 1
- OEPOKWHJYJXUGD-UHFFFAOYSA-N 2-(3-phenylmethoxyphenyl)-1,3-thiazole-4-carbaldehyde Chemical compound O=CC1=CSC(C=2C=C(OCC=3C=CC=CC=3)C=CC=2)=N1 OEPOKWHJYJXUGD-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 208000019300 CLIPPERS Diseases 0.000 description 1
- 102400000888 Cholecystokinin-8 Human genes 0.000 description 1
- 101800005151 Cholecystokinin-8 Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 229920000153 Povidone-iodine Polymers 0.000 description 1
- 239000004820 Pressure-sensitive adhesive Substances 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000010609 cell counting kit-8 assay Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 208000021930 chronic lymphocytic inflammation with pontine perivascular enhancement responsive to steroids Diseases 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 230000003779 hair growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000010832 independent-sample T-test Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000002132 lysosomal effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000009336 multiple cropping Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000037368 penetrate the skin Effects 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920006264 polyurethane film Polymers 0.000 description 1
- 229960001621 povidone-iodine Drugs 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000003684 theca cell Anatomy 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 210000003556 vascular endothelial cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/24—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/00051—Accessories for dressings
-
- A61F13/01017—
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive plasters or dressings
- A61F13/0259—Adhesive plasters or dressings characterised by the release liner covering the skin adhering layer
- A61F13/0266—Adhesive plasters or dressings characterised by the release liner covering the skin adhering layer especially adapted for wound covering/occlusive dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F2013/00089—Wound bandages
Abstract
The invention relates to a method for in vivo wound surface anoxic treatment. The method comprises the following steps of cutting a polyvinylidene chloride membrane to cover a wound surface according to the size of the wound surface, sealing the wound surface for 6 hours after using a medical operative membrane to fix, and then removing the polyvinylidene chloride membrane and the medical operative membrane. The method can maximize the positive role of in vivo anoxia on wound surface repairing cells, and is beneficial to accelerating wound surface healing; the animal experiment proves that the method can be applied to the full skin defective wound surface, the wound surface is protected, and the infection probability is reduced. The method also has the advantages of good safety, easiness in operation, low energy consumption and low cost.
Description
Technical field
The present invention relates to skin wound recovery technique, in particular it relates to a kind of method in body wound surface anoxic treatment。
Background technology
Skin is the organ that human body is maximum; it it is the important natural cover for defense of protection body; body can be made from the invasion and attack of physical property, chemical, mechanicalness and pathogenic microorganism, it is also possible to help the environment of body to remain stable for, play an important role in the maintenance of body stable state。After serious burn, the integrity of skin is destroyed, barrier action is lost, may result in a series of problems such as unbalance, the shock of Water-Electrolyte, very easily infecting, metainfective wound surface can become the important sources of body systemic infection, and therefore the treatment situation of wound surface can affect conditions of patients change largely, therefore for patients, wound closure is the important step for the treatment of as early as possible。
After serious burn, body can be lost a large amount of body fluid, ECBV and tissue perfusion and reduce。On this basis, wound surface local vascular is damaged, is infected, edge of wound cellular activity such as needs at the factor combined effect, causes oxygen-supplying amount to reduce, oxygen consumption increase, therefore engenders anaerobic environment around wound surface, hinders latter 3 days anoxias and reaches peak。Rodriguez points out, wound surface forms rear keratinocyte and do not immediately begin to migrate, but just starts after postponing a period of time to migrate, and micro-environmental hypoxia is likely to play an important role in the startup that epidermis cell migrates。Caitlin, Raja etc. point out that hypoxia in vitro can pass through number of mechanisms and promote the motor capacity of keratinocyte, and the research of many people such as LiW, DanielRJ, Humar, Lokmic then proves that the acute anoxia of wound surface passes through the propagation and the migration that promote epidermis cell, fibroblast and vascular endothelial cell thus promoting the healing of wound surface。Therefore wound surface formed descendant be shift to an earlier date ischaemia environment formation or can the faster wound repair corelation behaviour such as active cell migration, accelerate wound, and current research all concentrates on the hypoxia in vitro impact on wound repair corelation behaviour, lack anoxia to the application at body wound surface。
During appropriateness anoxia, body, based on compensatory response, can induce several genes to express, and supplies if recovering normal oxygen in time, the gene outcome that induction produces exists and continues to play a role, and participates in the generation of the biological behaviours such as the migration of wound repair cell, propagation and new vessels。During severe depletion of oxygen, because energy supplies wretched insufficiency, cell occurs losing compensatory change, such as to cell membrane, mitochondrion and lysosomal damage。Mitochondrion after damage causes again further anoxia, both reciprocal causations, forms vicious cycle, and various kinds of cell structural damage makes body be unable to maintain that normal vital movement。Therefore hypoxic exposure and degree are very crucial on the impact of body, in this patent, the research different anoxia functions time is extremely important to the process of wound repair。At present, but without in body wound surface anoxic treatment technology。
Summary of the invention
The object of the invention is for providing a kind of method in body wound surface anoxic treatment。
For achieving the above object, the technical scheme is that
A kind of method in body wound surface anoxic treatment, it is characterised in that comprise the following steps:
1) all to operation apparatus, polyvinylidene chloride film and wound skin is carried out disinfection respectively；
2) being cut into by polyvinylidene chloride film and wound surface homomorphosis or similar shape, size can be completely covered wound surface flap coverage edge 1-4mm, is covered on wound surface by the polyvinylidene chloride film cut；
3) according to the shape of polyvinylidene chloride film, cutting medical drape, it is sized to 0.5-2cm bigger than the edge of polyvinylidene chloride film, and medical drape is cut out and wound surface homomorphosis or similar vacancy；
4) separate paper of the medical drape being had vacant position by band separates with upper layer film, is covered by upper layer film on polyvinylidene chloride film, the vacancy alignment wound surface of film；
5) polyvinylidene chloride film and upper layer film are fixed on wound surface upper 6 hour, remove polyvinylidene chloride film, wound surface is exposed in air, divests upper layer film。
Polyvinylidene chloride film is a kind of food preservative film, and its chemical constituent is polyvinylidene chloride (polyvinylidenechloride), is the polymer of dichloroethylene (vinylidene chloride)。
Medical drape is to be coated polyurethane film by medical pressure-sensitive adhesive, cover release paper again to form, it is a ultra-thin, moisture-inhibiting, transparent, fit operative membrane, a good gnotobasis can be created at operative site, be widely used in sterile surgical。
Further, the OTR oxygen transmission rate of polyvinylidene chloride film is 60cm3/(m2.24h.atm), carbon dioxide transmission rate is 310cm3/(m2.24h.atm), rate of perviousness is 12g/ (m2.24h)。
Further, in step (1), the sterilization of operation machinery is realized by High Temperature High Pressure。
Further, in step (1), the sterilization to polyvinylidene chloride film is to pass through Co60Irradiation realizes。
Further, in step (1), after all skin degermings of wound, remove skin surface impurity and oils and fats。So that xerosis cutis cleaning。
Further, in step (5), before and after fixing to polyvinylidene chloride film and upper layer film, smear pressure from the middle part of polyvinylidene chloride film and upper layer film to two ends with aseptic absorbent cotton。This step need to operate with caution, it is to avoid curling。
Further, in step (5), after divesting upper layer film, if there being cull, clear up with aseptic cotton carrier。
The beneficial effects of the present invention is:
The method can make wound surface local form anaerobic environment in advance, the faster wound repair corelation behaviour such as active cell migration, accelerate wound healing, and the method animal experiment proves that and can apply to full thickness dermal wounds, it is possible to protection wound surface, reduce and infect probability。The advantage that the method also has safety operation good, easy, energy consumption is low, cost is low。
Accompanying drawing explanation
Fig. 1 is the cytotoxicity experiment result of embodiment 1；
Schematic diagram is implemented in the operation that Fig. 2 is embodiment 3；
Fig. 3 processes wound surface different time sections wounds in mice healing state comparison diagram with polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) in embodiment 3 respectively；
Fig. 4 is the time comparison diagram that after processing wound surface 6h with polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) respectively in embodiment 3, wounds in mice heals required completely；
Fig. 5 is respectively with wounds in mice tissue repair situation comparison diagram after polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) film process wound surface 6h in embodiment 3。
Detailed description of the invention
Illustrated embodiment is to better present disclosure be illustrated, but is not that present disclosure is only limitted to illustrated embodiment。So embodiment is carried out nonessential improvement and adjustment according to foregoing invention content by those of ordinary skill in the art, still fall within protection scope of the present invention。
Embodiment 1 polyvinylidene chloride theca cell toxicity test
For verifying the cytotoxicity of the method material requested, test as follows。
1) by material requested through Co603 times are rinsed with phosphate buffer (PBS) after irradiation sterilization；
2) preparation of lixiviating solution: every cm2Sample adds the 1mL DMEM culture fluid containing 10% (mass fraction) hyclone (FBS), and at 37 DEG C of temperature, lixiviate 24 hours, obtain lixiviating solution；
3) take the logarithm trophophase Human keratinocytes strain HaCaT, and making concentration is 1 × 104The cell suspension of individual/mL, is inoculated in 96 orifice plates and cultivates, every hole 100 μ L, and cell attachment removes former culture medium after growing 24 hours；
4) experimental group: add step 2 by 100 μ L/ holes to the HaCaT cell cultivated) the middle lixiviating solution prepared；Matched group: add the DMEM culture medium containing 10% (mass fraction) hyclone of equivalent to the HaCaT cell cultivated, often group multiple cropping 4 hole；
5) cultivate six days, carry out cell proliferation detection (CCK8) experiment every day, remove original lixiviating solution or culture medium, and respectively the cell of experimental group and matched group is washed twice by new culture medium, then every porocyte adds 100 new for μ L culture medium and 10 μ LCCK8 reagent (cellcountingkit-8, purchased from Japan colleague), one hour is hatched at 37 DEG C, then microplate reader is utilized to detect the absorbance in every hole at 450nm wavelength place, draw cell growth curve, detect the cytotoxicity of polyvinylidene chloride film, polymethylpentene membrane sample lixiviating solution。
Cell growth curve is as shown in Figure 1。It will be seen from figure 1 that the Growth of Cells no significant difference of experimental group and matched group, therefore the polyvinylidene chloride film used by this method is without obvious cytotoxicity, safety is higher。
Embodiment 2 mice full thickness dermal wounds makes and draws materials
1) preparation of BALB/C mice (purchased from Third Military Medical University's Experimental Animal Center) full thickness dermal wounds model: weigh in the balance and take Mouse Weight, laboratory animal gives pentobarbital sodium lumbar injection (100mg/kg, the 0.01ml/g) anesthesia that mass fraction is 1%。Anaesthetize and after successfully, use electric hair clippers against the direction of hair growth, shave off the hair of mouse back, the depilatory cream that uniform application is appropriate on the skin of back of mice, wipes whole depilatory cream gently with dry cotton ball when hair can be taken off, and dips in warm water with cotton balls and clean up mouse skin。Mice is fixed on ventricumbent position, art district routine iodophor disinfection。Both hands have pinched mouse back skin, along the direction doubling of mice height, exert oneself from opposite side with the card punch of diameter 5mm, unclamp penetrate the skin of both sides until sensation card punch after。Remove the skin histology of perforation place with tweezers folder, unclamp skin, clamp skin edge portion with ophthalmic tweezers and just can completely take the skin laid off, it is seen that mouse back both sides are in the same size, the full thickness dermal of positional symmetry。Take BALB/C mice 18, prepare wound model all as stated above。The mice having made wound surface is individually raised, it is ensured that the water of abundance, food, activity freely, implements 12 hours systems round the clock。
2) wounds in mice tissue sampling: cervical dislocation puts to death mice, ophthalmic tweezers mentions the wound surface skin in body anoxia, it is about 1.5mm place with most advanced and sophisticated sharp eye scissors along edge of wound and cuts off skin, it is to avoid cutting repeatedly, tractive, required part of drawing materials avoids tweezers repeatedly to clamp。Wound surface specimen is taken off rear corium and is faced down, and is laid on filter paper, with tweezers gently by dermatological specimens peripheral extension, it is to avoid curl into fold。Cut, along the edge being sampled this, the filter paper that size is harmonious, skin histology is together placed in the vial filling 4% paraformaldehyde together with filter paper, is placed in 4 DEG C of preservations。Take off the wound week skin histology to oxygen group as usual by same method at mouse back, and process according to said method。Carry out HE dyeing, Masson dyeing after section, observe anoxia wound surface and normal oxygen wound tissue structure situation。
Embodiment 3 is in body wound surface anoxic treatment
1) eye scissors, ophthalmic tweezers etc. are operated apparatus and carry out autoclave sterilization；Polyvinylidene chloride film (Polyvinylidenechloride film, experimental group), polymethylpentene film (Polymethylpentene film, matched group) pass through Co60Radiation sterilization；Pick appropriate povidone iodine, all skin of sterilization wound with aseptic cotton carrier, remove skin surface impurity and oils and fats, make xerosis cutis clean；
2) observing wound surface shape and area, with eye scissors cutting polyvinylidene chloride film and polymethylpentene film, shape and wound surface homomorphosis or similar, size is can be completely covered respective wound surface flap coverage edge 2-3mm；By cutting cut polyvinylidene chloride film and polymethylpentene film carefully covers on wound surface respectively；
3) according to cutting polyvinylidene chloride film and the shape of polymethylpentene film, cutting medical drape, size 1cm bigger than the edge cutting polyvinylidene chloride film and polymethylpentene film；By the medical drape doubling of cutting, cut out and wound surface homomorphosis or similar vacancy；
4) separate paper of the medical drape being had vacant position by band separates with upper layer film, obtains upper layer film, operates with caution, it is to avoid curling；Being respectively overlay in by upper layer film on polyvinylidene chloride film and polymethylpentene film, its edge is covered by upper layer film completely, and the vacancy alignment wound surface of film central authorities, unnecessary film is attached at wound week skin；Before and after fixing to polyvinylidene chloride film and polymethylpentene film and upper layer film, smear pressure from the middle part of polyvinylidene chloride film and polymethylpentene film and upper layer film to two ends with aseptic absorbent cotton so that it is with skin apt, bonding closely, do not communicate with ambient atmosphere；Polyvinylidene chloride film and polymethylpentene film is kept to be fixed on 6h on respective wound surface；
5) removing polyvinylidene chloride film and polymethylpentene film, be exposed in air by wound surface, divest upper layer film, if there being cull, cleaning out with aseptic cotton carrier, be exposed in air by wound surface, observe wound healing situation, record wound surface heals required time completely；The operation of above step implements schematic diagram as shown in Figure 2；
6) utilize ImageProPlus6.0 that each group of wound surface photo is analyzed, calculate residual wound area (area that edge of wound surrounds)；
7) use spss13.0 software respectively two groups of wound surface are healed completely required time adopt independent samples t test carry out statistical analysis, result is as shown in the table:
Table 1 wound surface heal completely required time (my god)
| Matched group | 13.67±0.48 |
| Experimental group | 10.14±0.95 |
Fig. 3 is for process wound surface different time sections wounds in mice healing state comparison diagram with polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) respectively。As can be seen from Figure 3: mice is compared with the respective matched group wound surface of experimental group wound surface and its, and healing rate is substantially accelerated。This facilitation is closely related with hypoxic exposure, along with the prolongation of hypoxic exposure, the promotion effect of wound repair is gradually increased and little reaches peak constantly processing 6, extending hypoxic exposure subsequently and can reduce the facilitation to wound repair on the contrary。
The time comparison diagram that Fig. 4 heals required for wounds in mice after processing wound surface 6h with polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) respectively completely。From fig. 4, it can be seen that experimental group substantially shortens at body wound surface anoxic treatment 6h relatively matched group healing time。
Taking the skin histology after the healing of experimental group and matched group to be analyzed, Fig. 5 is for processing wounds in mice tissue repair situation comparison diagram after wound surface 6h with polyvinylidene chloride film (Polyvinylidenechloride film) and polymethylpentene film (Polymethylpentene film) respectively。As can be seen from Figure 5, HE, Masson dyeing is shown in that the layering of two groups of skins is all very clear, the more normal oxygen place of epidermal thickness of anoxic treatment wound surface substantially increases, and the propagation of prompting keratinocyte increases, without skin appendages such as hair follicle, sebaceous gland, sweat glands in skin corium, reticular layer is thick, the a large amount of hypertrophy of visible collagen fiber, thickness not etc., arrangement densification, the substrate being around homogenised is surrounded, and has no notable difference。After being also shown in anoxic treatment wound healing in Masson dyeing, subcutaneous number of blood vessel is higher。
In addition, it is to be understood that, although this specification is been described by according to embodiment, but not each embodiment only comprises an independent technical scheme, this narrating mode of description is only for clarity sake, description should be made as a whole by those skilled in the art, and the technical scheme in each embodiment through appropriately combined, can also form other embodiments that it will be appreciated by those skilled in the art that。
Claims (7)
1. the method in body wound surface anoxic treatment, it is characterised in that comprise the following steps:
(1) all to operation apparatus, polyvinylidene chloride film and wound skin is carried out disinfection respectively；
(2) being cut into by polyvinylidene chloride film and wound surface homomorphosis or similar shape, size can be completely covered wound surface and wound surface edge 2-3mm, is covered on wound surface by the polyvinylidene chloride film cut；
(3) according to the shape of polyvinylidene chloride film, cutting medical drape, size 1cm bigger than the edge of polyvinylidene chloride film, and medical drape is cut out and wound surface homomorphosis or similar vacancy；
(4) separate paper of the medical drape being had vacant position by band separates with upper layer film, is covered by upper layer film on polyvinylidene chloride film, the vacancy alignment wound surface of film；
(5) polyvinylidene chloride film and upper layer film are fixed on wound surface upper 6 hour, then remove polyvinylidene chloride film, wound surface is exposed in air, divests medical drape。
2. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterised in that: the OTR oxygen transmission rate of polyvinylidene chloride film is 60cm3/(m2.24h.atm), carbon dioxide transmission rate is 310cm3/(m2.24h.atm), rate of perviousness is 12g/ (m2.24h)。
3. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterised in that: in step (1), the sterilization of operation apparatus is realized by High Temperature High Pressure。
4. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterised in that: in step (1), the sterilization to polyvinylidene chloride film is to pass through Co60Irradiation realizes。
5. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterised in that: in step (1), after all skin degermings of wound, remove skin surface impurity and oils and fats。
6. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterized in that: in step (5), before and after fixing to polyvinylidene chloride film and upper layer film, smear pressure from the middle part of polyvinylidene chloride film and upper layer film to two ends with aseptic absorbent cotton。
7. a kind of method in body wound surface anoxic treatment according to claim 1, it is characterised in that: in step (5), after divesting upper layer film, if there being cull, clear up with aseptic cotton carrier。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610044233.0A CN105688253A (en) | 2016-01-22 | 2016-01-22 | Method for in vivo wound surface anoxic treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610044233.0A CN105688253A (en) | 2016-01-22 | 2016-01-22 | Method for in vivo wound surface anoxic treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105688253A true CN105688253A (en) | 2016-06-22 |
Family
ID=56228367
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610044233.0A Pending CN105688253A (en) | 2016-01-22 | 2016-01-22 | Method for in vivo wound surface anoxic treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105688253A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2652361Y (en) * | 2003-09-26 | 2004-11-03 | 威海赛绿特科技发展有限公司 | Full degradation type medical dressing sheet |
| WO2008155659A2 (en) * | 2007-04-24 | 2008-12-24 | Yaojiong Wu | Compositions for preventing or treating skin defects and methods of use thereof |
-
2016
- 2016-01-22 CN CN201610044233.0A patent/CN105688253A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2652361Y (en) * | 2003-09-26 | 2004-11-03 | 威海赛绿特科技发展有限公司 | Full degradation type medical dressing sheet |
| WO2008155659A2 (en) * | 2007-04-24 | 2008-12-24 | Yaojiong Wu | Compositions for preventing or treating skin defects and methods of use thereof |
Non-Patent Citations (2)
| Title |
|---|
| HITOMI SANO ET AL.: "Influence of Oxygen on Wound Healing Dynamics: Assessment in a Novel Wound Mouse under a Variable Oxygen Environment", 《PLOS ONE》 * |
| 闫甜甜: "短时间低氧处理对创面愈合的影响及其机制研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vig et al. | Advances in skin regeneration using tissue engineering | |
| JP4555576B2 (en) | Surgical device for skin treatment or examination | |
| RU2524619C2 (en) | Method for making dermal matrix | |
| SG187260A1 (en) | Devices for harvesting a skin graft | |
| CN102552099A (en) | Composite biological agent used for skin beautifying and restoring and preparing method | |
| CN105132358B (en) | Method for obtaining tissue engineering epidermis by culture and application thereof | |
| EP1226234B1 (en) | Hair transplantation | |
| Frączek et al. | Surgical treatment of vitiligo | |
| CN108452374A (en) | A kind of spider silk composition and preparation method thereof | |
| CN105688253A (en) | Method for in vivo wound surface anoxic treatment | |
| WO2014117597A2 (en) | Rapid tissue engineering method for skin construction and repair of skin damage | |
| CN103636596B (en) | A kind of biomembrane protection liquid and preparation and application thereof | |
| US20240052308A1 (en) | Human scalp hair follicle single cell suspension, preparation method and application thereof | |
| CN103301153A (en) | Application of umbilical cord mesenchymal stem cell culture solution or product prepared from umbilical cord mesenchymal stem cell culture solution in preparation of medicament for treating skin wound | |
| Sachkov et al. | Use of cadaver skin in the treatment of wounds | |
| NOVOTNA et al. | Decellularization techniques of human foreskin for tissue engineering application | |
| CN206103015U (en) | Organizational project skin founds apparatus system that combines difficult healing nature surface of a wound of negative pressure drainage treatment | |
| Imahara et al. | Skin grafts | |
| RU2657202C1 (en) | Method of cutaneous autoplasty | |
| RU2373944C1 (en) | Method of burn wound repair | |
| RU2685472C2 (en) | Method of preparing autologous monocytes of human for treating burn wounds | |
| CN103476360B (en) | Hair transplant material | |
| Sawatkar et al. | Follicular cell suspension: A new surgical modality for the treatment of vitiligo | |
| Louri et al. | MELANOCYTE-KERATINOCYTE TRANSPLANTATION IN POST-BURN LEUKODERMA SCARS: PRELIMINARY EXPERIENCE USING A MODIFIED TECHNIQUE. | |
| CN105709276A (en) | Chorion decellularizing liquid and decellularizing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160622 |
|
| RJ01 | Rejection of invention patent application after publication |