CN105687957B - 一种防治鸡非典型新城疫的中药制剂 - Google Patents

一种防治鸡非典型新城疫的中药制剂 Download PDF

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CN105687957B
CN105687957B CN201610112741.8A CN201610112741A CN105687957B CN 105687957 B CN105687957 B CN 105687957B CN 201610112741 A CN201610112741 A CN 201610112741A CN 105687957 B CN105687957 B CN 105687957B
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newcastle disease
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赵坤
徐萍
银梅
徐小博
刘明成
王丽荣
李任峰
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Henan Institute of Science and Technology
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Abstract

一种防治鸡非典型新城疫的中药制剂，本发明的技术方案是，一种防治鸡非典型新城疫的中药制剂，（1）选取：茵陈、百部、大青叶、紫花地丁、板蓝根、紫草、栀子、黄连、金银花、牛蒡子、黄芪、白术、防风、五味子、甘草；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。本发明的按照传统中兽医学的君臣佐使理论进行组方，配伍合理，药材易得，制备方法简单；本品为纯中药制剂，应用于养鸡生产中，超微粉碎、易于吸收，添加量少、成本低，无毒副作用、无残留、符合安全绿色食品理念。

Description

一种防治鸡非典型新城疫的中药制剂
技术领域：
本发明涉及防治鸡非典型新城疫，特别是一种防治鸡非典型新城疫的中药制剂。
背景技术：
新城疫(Newcastle Disease，ND)，又称亚洲鸡瘟、伪鸡瘟等，是发生于鸡、鸽、鹌鹑、火鸡等家禽和野生禽类的一种急性、败血性和高度接触性传染病，是世界动物卫生组织（OIE）规定的法定必报传染病之一。其病原是新城疫病毒(Newcastle Disease Virus，NDV)。新城疫病毒（NDV）属于副黏病毒科，副黏病毒亚科，腮腺炎病毒属（Rubulavirus）。新城疫病毒（NDV）只有1个血清型，但通过遗传发生分析，可将不同地区的流行毒株或疫苗株分成若干个基因型或抗原群。新城疫不仅给家禽养殖业带来严重的经济损失，而且还严重影响禽和禽类相关产品的国际贸易。因此，世界各国对该病的发生流行均采取非常严格的防控措施。目前控制新城疫主要依靠疫苗接种，但由于抗原变异、疫苗选择不当、接种程序不合理等因素影响，并不能完全有效控制本病的发生。1990年代以来，我国非典型新城疫在免疫鸡群中的发生逐渐严重，主要特征是临床症状和病理变化不明显，任何日龄的鸡都可发病，发病率、死亡率低，产蛋量大幅度降低，抗体滴度高。这种非典型新城疫严重危害了鸡的生产性能，给养禽业造成了巨大损失。
发明内容：
本发明的目的是提供一种治疗效果显著、加工方法便捷的防治鸡非典型新城疫的中药制剂。
本发明的技术方案是，一种防治鸡非典型新城疫的中药制剂，其特征在于：（1）选取重量份为：茵陈4-6份、百部7-9份、大青叶13-17份、紫花地丁13-17份、板蓝根14-18份、紫草9-12份、栀子13-16份、黄连7-9份、金银花8-12份、牛蒡子7-9份、黄芪11-15份、白术7-9份、防风3-5份、五味子3-5份、甘草3-5份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。本发明所述的组方中所涉及的各种中药材按照常规的中药材加工方法进行加工，如清洗除杂、干燥等。
本发明的按照传统中兽医学的君臣佐使理论进行组方，配伍合理，药材易得，制备方法简单；本品为纯中药制剂，应用于养鸡生产中，超微粉碎、易于吸收，添加量少、成本低，无毒副作用、无残留、符合安全绿色食品理念。
具体实施方式：
实施例1，
一种防治鸡非典型新城疫的中药制剂，（1）选取重量份为：茵陈4份、百部7份、大青叶13份、紫花地丁13份、板蓝根14份、紫草9份、栀子14份、黄连7份、金银花8份、牛蒡子7份、黄芪11份、白术8份、防风3份、五味子4份、甘草4份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。
实施例2，
一种防治鸡非典型新城疫的中药制剂，（1）选取重量份为：茵陈5份、百部8份、大青叶15份、紫花地丁15份、板蓝根15份、紫草10份、栀子15份、黄连8份、金银花10份、牛蒡子8份、黄芪12份、白术8份、防风3份、五味子3份、甘草3份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。
实施例3，
一种防治鸡非典型新城疫的中药制剂，（1）选取重量份为：茵陈6份、百部9份、大青叶17份、紫花地丁17份、板蓝根18份、紫草12份、栀子16份、黄连9份、金银花12份、牛蒡子9份、黄芪15份、白术9份、防风5份、五味子5份、甘草5份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。
实施例4，
一种防治鸡非典型新城疫的中药制剂，（1）选取重量份为：茵陈5份、百部8.5份、大青叶16份、紫花地丁16份、板蓝根16份、紫草11份、栀子13份、黄连9份、金银花9份、牛蒡子7.5份、黄芪13份、白术7份、防风4份、五味子5份、甘草4.5份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。
本发明，安全性试验：
选取20日龄SPF雏鸡10只，用依据本发明实施例2所制备的中药制剂，按5倍治疗剂量（按照鸡自身体重每千克每天用药1.5g这一标准）添加于饲料中，连续使用5天，同时，设空白对照。结果表明，用药期间，试验组鸡只无任何不良反应，正常采食和饮水，日增重与对照组差异不显著。
预防效果试验：
选取20日龄未免疫新城疫的健康雏鸡30只，分为3个组，按1.5g/（日.千克体重）的剂量添加饲料中，5日后开始攻毒，第1组使用NDV xx09毒株（该毒株保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC NO.11290），点眼、滴鼻。第2组使用NDVF48E9株滴鼻、点眼。第3组为空白对照。攻毒期间继续饲喂添加中药的饲料。观察发现，第1组48小时后开始出现鸡只轻度精神沉郁，72小时后逐渐恢复正常。第2组48小时后开始出现沉郁、咳嗽、采食量下降等发病症状，攻毒后第4日死亡1只，其他鸡逐渐恢复正常。结论：本中药制剂对新城疫有一定预防作用。
治疗案例1：
新乡某养鸡户，已按免疫程序进行新城疫免疫的4000只8月龄伊莎蛋鸡发病，产蛋量下降20%左右，临床表现为：精神沉郁，呆立不动，食欲下降，部分鸡采食时做吞咽或甩头动作，鸣叫声减弱，有喘鸣声，发病鸡鸡冠萎缩，颜色由鲜红色逐渐变成浅红色乃至苍白色，每天死亡8-11只。剖检发现，肝脾肾不同程度肿大；心包积液，心冠脂肪有点状出血，心肌色淡；肌胃内膜易剥离，有出血斑，腺胃乳头水肿；泄殖腔轻微出血，胸腔积液，腹水增多。结合实验室诊断确定为非典型新城疫。养殖户采用本发明实施例2中药制剂进行治疗，治疗第4天起无死亡病例，采食量基本恢复，产蛋量开始回升，至第7天鸡群基本恢复正常。
治疗案例2，
修武县某鸡场，10月龄海兰褐商品蛋鸡4500只。1周龄和7周龄时分别以新城疫IV系苗点眼滴鼻进行免疫，9周龄时以新城疫I系苗肌注接种。10月龄时开始发病，发病率75％。病鸡主要表现为产蛋率下降，由原来的80％下降到50％。病鸡临床表现为轻微呼吸道症状，一部分病鸡出现甩头等神经症状，排绿色稀便，多数鸡冠苍白。薄壳蛋、软壳蛋等畸形蛋增多，由原来的约2kg增加到约4kg，并出现零星死亡。剖检病死鸡，可见气管环轻微出血，肌胃内膜易剥离、少量出血点，腺胃黏膜稍有水肿、有出血斑，泄殖腔、卵泡有轻微出血。结合实验室诊断，确诊为非典型新城疫。应用本发明实施例2中药制剂进行治疗，治疗第5天起死亡2只，采食量开始增多，产蛋量逐渐回升，至第8天鸡群基本恢复正常。
治疗案例3，
卫辉市某养鸡户，饲养海蓝褐蛋鸡3000只，开始产蛋已月余。发病首日个别鸡只出现呼吸道症状，喘鸣、呼噜、咳嗽；次日大群开始发病，产蛋量开始下降。之后产沙皮蛋、软壳蛋等畸形蛋增多。吃料量减少，拉绿便，喝水增多。神经症状病鸡增多。每天死亡数量增加。剖检病死鸡，可见病鸡气囊浑浊，气管环有出血点，嗉囊有积水，胆囊充盈，有的肝脏黑紫色，多数病例腺胃变软，少数腺胃有出血点，肌胃充满饲料，小肠弥漫性出血，泄殖腔、直肠有点状、刷状出血。采集病料进行实验室诊断，确诊为非典型新城疫。应用本发明实施例2中药制剂进行治疗，治疗第4天起死亡病例减少至1例，第5天产蛋量开始回升，采食量明显增多，至第7天鸡群基本恢复正常。
上述实施例仅仅是为清楚地说明所作的举例，而并非对实施方式的限定。对于所属领域的普通技术人员来说，在上述说明的基础上还可以做出其它不同形式的变化或变动。这里无需也无法对所有的实施方式予以穷举，而由此所引伸出的显而易见的变化或变动仍处于本发明创造的保护范围之内。

Claims (1)

1.一种防治鸡非典型新城疫的中药制剂，其特征在于：（1）选取重量份为：茵陈4-6份、百部7-9份、大青叶13-17份、紫花地丁13-17份、板蓝根14-18份、紫草9-12份、栀子13-16份、黄连7-9份、金银花8-12份、牛蒡子7-9份、黄芪11-15份、白术7-9份、防风3-5份、五味子3-5份、甘草3-5份；（2）将步骤（1）中选取的各药物分别研磨后进行超微粉碎，粉碎后的药粉过500目筛，再将筛下物进行混合、搅拌，完成制备。
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