CN105685491A - Composition and method for preventing or treating animal obesity - Google Patents

Abstract

The invention relates to a composition and method for preventing or treating animal obesity, and discloses a composition for preventing or treating the animal obesity. The composition comprises an appropriate amount of general alkaloids of capsicum annuum, preferably capsaicin or active metabolites thereof, for effectively preventing or treating the animal obesity. The invention further discloses a method for preventing or treating the animal obesity and application of the composition.

Description

The compositions of prevention or treatment obesity in animals and method

The application is the applying date is on February 23rd, 2007, application number is 200780052684.8 (international application no is PCT/US2007/062714), and denomination of invention is the divisional application of the application for a patent for invention of " compositions of prevention or treatment obesity in animals and method "。

Invention field

The present invention relates to the compositions of prevention and/or treatment obesity in animals and method。

Background of invention

The fact that unfortunate it is that many animals, particularly companion animals (such as Canis familiaris L. and cat) is more heavy than its ideal body。With excess fat tissue, these animals are likely to be diagnosed as such as " fat ", " overweight " or " obesity " clinically, therefore relevant SOM disease is more likely suffered from, such as arthritis, heart disease, respiratory tract disease, diabetes, bladder cancer, hypothyroidism, arthrosis and/or pancreatitis。

The trial of the excess fat tissue mass of prevention or minimizing companion animals generally includes diet control and exercise。It practice, have been developed over the many heats " low " for overweight companion animals or " reduction " food and commercially available for controlling calorie intake, preventing body weight from increasing or promote to lose weight。Although this kind of pet food formulations can obtain, but, it is still desirable to kit is containing being used for preventing or treating other formula of the innovation composition of companion animals obesity and nutrient。

Using canid animal cell to cultivate chromosome set method of inspection, we have determined that can the material of expression of the various Canis animals gene of external change。According to these data and recent show to be likely to change in energy (heat) limit procedure discovery (Higami, Y et al., the J.Nutr.2006Feb of the expression of some gene mice；136 (2): 343-52), we have determined that the list that can be used for preventing or treat the material of companion animals obesity。Specifically and as described below, the intra-body data collected from research of ingesting shows, the compositions comprising capsaicin is particularly effectively prevented or treats obesity in animals。

Capsaicin is known as the alkaloid in " heat " in Fructus Capsici (chilipepper) or pungent source, uses in many ways, including such as, as insecticide and animal repellant and be used in medical emulsifiable paste and gel。In addition, United States Patent (USP) 5,273,754 disclose and comprise " pyrogenicity wind dispelling material (heatingcarminativesubstance) " by oral, preferred capsaicin, and the method that the appetite suppressant compositions of " refrigeration wind dispelling material (coolingcarminativesubstance) " reduces appetite。But, the not open compositions comprising capsaicin without refrigeration carminative (such as Herba Menthae, Herba Menthae Rotundifoliae, menthol) of this list of references, also without the open compositions comprising capsaicin as animal feed。

Summary of the invention

The present invention relates to the compositions that can be used for preventing and/or treat obesity in animals。Described compositions comprises the material of the expression of one or more genes relevant to energy restriction that can change (such as raise or lower) animal。Therefore, in an aspect, the present invention relates to effectively to prevent or treat the compositions that the amount of obesity in animals comprises Capsaicinoids or its active metabolite。In one embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition can further include in soluble fiber, methionine, eicosapentaenoic acid (EPA) or docosahexenoic acid (DHA) is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In another aspect, the present invention relates to by effectively to prevent or to treat the amount of obesity of described animal to use the method that the compositions comprising the material that can change the expression that one or more of (such as raise or lower) animal limit relevant genes with energy is prevented and/or treated obesity in animals to described animal。In one embodiment, said composition comprises Capsaicinoids or its active metabolite。In one embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition comprised in soluble fiber, methionine, EPA and DHA further is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In another further aspect, the present invention relates to the compositions effectively causing the amount of the self regulation of ingesting of animal to comprise Capsaicinoids or its active metabolite。In one embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition comprised in soluble fiber, methionine, EPA and DHA further is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In another aspect, the method that the present invention relates to the self regulation of ingesting causing animal, use, to described animal, the compositions comprising Capsaicinoids or its active metabolite including with the amount of self regulation of ingesting described in effectively causing。In one embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition comprised in soluble fiber, methionine, EPA and DHA further is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In another further aspect, the present invention relates to Capsaicinoids or its active metabolite purposes in the manufacture of prevention or the compositions for the treatment of obesity in animals。In special embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition can further include in soluble fiber, methionine, EPA and DHA is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In another further aspect, the present invention relates to Capsaicinoids or its active metabolite purposes in the manufacture causing the compositions of self regulation of ingesting of animal。In special embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition can further include in soluble fiber, methionine, EPA and DHA is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

In further aspect, the present invention relates to the compositions of present invention purposes in the method for the method of prevention as detailed in this article or treatment obesity in animals and the self regulation of ingesting causing animal。

In another further aspect, the present invention relates to the external member of the compositions comprising the present invention。

Detailed description with reference to following Examples understands other features and advantages of the present invention。

Detailed Description Of The Invention

Invention as described herein is estimated to be not limited to described adhoc approach, code and reagent, because these are variable。It will further be understood that term used herein is only used for describing special embodiment rather than the scope to be intended to limit the present invention in any manner。

Unless otherwise specified, all technology used herein and scientific terminology have the identical meanings that those skilled in the art are commonly understood by。All patents, patent application, publication and other list of references quoting herein or mention are incorporated herein by this reference for various purposes。

Unless clearly indicated separately in literary composition, singulative " " and " being somebody's turn to do " herein and used by claims include plural reference。

Term used herein " overweight ", " fat ", " obesity ", " obesity " and similar terms refer to greater than the weight of animals situation of its ideal body weight。Such as, can refer to use animal care professional and the known technology of others skilled in the art and method to be defined as any animal with excess body fat tissue or the animal being easily generated excess body fat tissue for the term " fat " of animal。Such as, if (1) animal has the Body Mass Index (BMI) of 25 or bigger (being considered to include the numerical value of " overweight " and " obesity " animal in the certain methods characterizing animal state), (2) the weight of animals is than its " ideal " body weight high 15% or more that defined by animal care professional or well known by persons skilled in the art, (3) it is 27% or bigger by the DEXA animal body percent fat recorded, or (4) animal has by those skilled in the art's use " SmallAnimalClinicalNutrition ", 4th edition, 13rd chapter (ISBN0-945837-05-4) or its use the body condition mark (BCS) in the scale of 1 to 5 more than 3 that the method disclosed in the counterpart of other BCS method determines, then this animal is considered " fat "。In some cases, than ideal body weight high 20% or more animal be considered fat。About the method and composition of the present invention, term used herein " obesity " includes to be considered as any weight status more than this animal ideal body weight by those skilled in the art。

The present invention relates to any animal, it is preferable that mammal, more preferably companion animals。Term " companion animals " refers to any animal being intimately living together with people, and includes, but not limited to Canis animals and the felid of any kind。Such as, it is contemplated herein that, this term can also include diet and people can be subject to control and may benefit from any animal of feed formulations disclosed herein。These animals can include, for instance domesticated farm animals (such as, cattle, horse, pig etc.) and be housed in the non-performing animal in such as zoo etc. and analog。

" effectively the amount of prevention or treatment obesity " used herein, " effective dose " and similar terms refer to the amount that can effectively realize the compound as herein described of particular biological result, material or compositions。This kind of result can include, but not limited to treatment and/or the prevention of obesity。Can such as by realizing this effective active to the compositions needing its animal to use the present invention。

The material of any name used herein not only includes this material, also includes its any chemistry counterpart, active metabolite or salt, and this depends on the circumstances。Such as, term provided herein " vitamin " is intended to include any and all chemical species or the counterpart that are suitable for preparing these compounds in the present compositions that those skilled in the art easily determine。

" Capsaicinoids " used herein refer to generally with the fruit of capsicum plants, for instance the compound that " heat " that Fructus Capsici links together or pungent sense are responsible。This compounds includes, and capsaicin (that is, 8-methy-N-vanillyl-6-nonenamide), dihydrocapsaicin, Nordihydrocapsaicin, homocapsaicin and Homodihydrocapsaicin, their structure and chemical formula are that those skilled in the art are familiar with。This term used herein includes the Capsaicinoids (such as, be isolatable from Fructus Capsici or Fructus Capsici powder) and their any and all synthesized form that naturally occur, and their any salt or metabolite。The character of Capsaicinoids can be as powder, oil, resin or other form applicable and compositions disclosed herein preparation。Commercial form includes, such as, CapsicumfrutescensL. or the dry fruit of CapsicumannuumL. real, such as can as " oleoresincapsicum; Africantype " (in the examples below that referred to as " Fructus Capsici ") (KALSEC, Kalamazoo, MI) buy。As contemplated herein, the compositions of the present invention can comprise one or more Capsaicinoids。

" soluble fiber " used herein refers to and absorbs water in digestion process and slow down Nutrients Absorption speed and be typically found in such as Herba bromi japonici bran, seed, bean and some fruits and vegetables, the dietary fiber in beet pulp, guar gum, root of Herba Cichorii, Herba Plantaginis, pectin, blue berry, mossberry, Fructus Cucurbitae moschatae, Fructus Mali pumilae, Herba bromi japonici, bean, Citrus, Fructus Hordei Vulgaris or Semen Pisi sativi。This term used herein comprises the soluble fiber in the apparent any source suitable in compositions disclosed herein of those skilled in the art。

Term used herein " changes the expression of one or more genes relevant to energy restriction of animal " and refers to the rise of the mRNA level in-site of gene or lower so that the level of the protein of these gene codes can correspondingly be changed, thus the weight of animals is produced beneficial effect。Not being bound by any particular theory, the change of expression is attributable to the change of the stability of mRNA transcription product and/or the change of actual transcriptional level。

" gene relevant to energy restriction of animal " refers to those genes reformed in the animal body being subject to energy or heat restriction。Not being bound by any particular theory, the example of this genoid includes Higami, Y. et al. J.Nutr.2006Feb；In 136 (2): 343-52 discuss those and be believed to comprise the gene relevant to inflammation, cytoskeleton, extracellular matrix and angiogenesis。Table 1A disclosed below lists some corresponding Canis animals genes, and it expresses external change under various compounds exist；Capsaicin disclosed herein can several (indicating with " * ") in these genes of external change。

Table 1A

The gene (Higami et al.) relevant to energy restriction of mice,Also due to external being exposed in compound changes in canid animal cellComplement, their regulon and their receptor

C1Q. A chain

Complement component 4*

CD59 antigen

Glycoprotein rich in histidine

Interferon regulation transcription factor and interferon inducible protein

The homologue * of Fragilis/ rat IFN inducible protein

The homologue * of the 35-kDa albumen that human interferon brings out

Acute phase protein

Hoptoglobin

Serum amyloid A 3 albumen

MHC and regulon thereof

Albumen containing ankyrin relevant for regulatory factor X-

Protein degradation

Cathepsin S

Ctsz

Cell type-specific

Many cross-films (multitransmembrane) albumen that lysosome is relevant

CD81 antigen

The macrophage protein 1 that natural resistance is relevant

^αCytochrome b5 58/ cytochrome b-245*

CD68 antigen

Containing the module of EGF-sample, the hormone receptor 1 of mucin-sample；EMR1

TYRO protein tyrosine kinase associated proteins；TYROBP

Fyn proto-oncogene

Inflammation, other

Annexin A1

MD-1/ lymphocyte antigen 86

Tubulin

^αTubulin, 1*

^αTubulin, 6

Tubulin, β 2

Tubulin, β 3

Tubulin, β 5

Other cytoskeleton

^γActin, cytoplasmic

Spectrin, β, brain

Actin-adjustment

^εChaperonin (Chaperonin) containing Tcpl subunit

Actin associated protein 2/3 complex, subunit 2,34kDa

Plastin (Plastin)/lymphocyte cytosolic protein 1*

Phemerol chloride (Septin) 9

Thymosin, β 10

Collagen

^αPrecollagen, I type, 1

^αPrecollagen, V-type, 1

Collagen regulates

Lysyloxidase；Lox

Other extracellular matrix

Nestin 2；NID2/ bone nestin

Fibulin2

Cell adhesion

^δSarcoglycan,

DNA binding inhibitors 1

^αOxygen deficient induction factor 1, subunit

Extracellular matrix

TSP-1

Protein is changed

Mesoderm specific transcriptional product

Serpin, clade B, member 6

The present invention is based on being surprisingly found that, namely in vitro, Cucumber can change the expression of (such as raise or lower) many Canis animals genes, these genes also change in mice in energy limit procedure, intra-body data disclosed herein particularly indicates that and comprises Capsaicinoids, for instance the compositions of capsaicin can be used for treating or preventing obesity in animals。Specifically, any particular theory or binding mode by the present invention are not limited, and data show, add the automatic minimizing that capsaicin can cause companion animals to ingest in food compositions。Therefore, in a specific aspect, the present invention relates to the compositions that can be used for preventing and/or treat obesity in animals, said composition is effectively to treat or to prevent the amount of obesity in animals to comprise Capsaicinoids, it is preferable that capsaicin。In another aspect, the present invention relates to prevention and/or the method for the treatment of obesity in animals, comprise Capsaicinoids including effectively to treat or to prevent the amount of obesity in animals to use to this animal, it is preferable that the compositions of capsaicin。

" self regulation of ingesting of animal " used herein refer to a certain amount of food of animal consumption until be satiated with food (saited) and reuptake beyond the tendency of more foods (this can cause obesity) of this amount。Such as, this tendency can show as this animal (such as Canis animals) and leave still bowl containing food, and compared with during with other food compositions of feed, this animal obtains satiety after the compositions absorbing the less amount of present invention。For felid (it is generally only and is stayed by food in bowl hereafter to return at any time to eat again), think that the compositions of the present invention can cause the overall minimizing of the total foodstuff amount of actual picked-up when patronizing its bowl every time of this felid herein。

" composition " used herein refers to any component of compositions。" nutrient " refers to the material providing nutrition。In some cases, a composition can comprise more than one " nutrient ", for instance, compositions can comprise fish oil as composition, and this oil itself comprises important nutrient, such as EPA and DHA。The difference of these terms is that those skilled in the art are familiar with。

As imagined herein, the compositions of the present invention is intended to include complete in nutrition and balance animal feed composition, and it additionally comprises Capsaicinoids, it is preferable that capsaicin。In in other, it is any or multiple that the compositions of the present invention can further include in soluble fiber, methionine, EPA and DHA。

In nutrition, complete and balance pet food composition is that those skilled in the art are familiar with。Such as, material suitable in animal feed composition that is complete in nutrition and that balance, as nutrient and composition and recommended amounts thereof are found in such as theOfficialPublicationoftheAssociationofAmericanFeedCont rolOfficials, Inc. (AAFCO), Atlanta, GA2005 or theNationalResearchCouncil ' sNutrientRequirementsofDogsandCats, TheNationalAcademyPress, Washington, D.C., 2006。

Such as, the nutrition of the present invention can comprise with the dog food composition of balance completely:

By weight about 0 to about 90%, it is preferable that the carbohydrate of about 5% to about 45%；

By weight about 5% to about 70%, it is preferable that the protein of about 10% to about 60%；

By weight about 2% to about 50%, it is preferable that the fat of about 5% to about 40%；

By weight about 0.1% to about 20%, it is preferable that the total dietary fiber of about 1% to about 11%；

By weight about 0 to about 15%, it is preferable that other nutrient of the vitamin of about 2% to about 8% and mineral, antioxidant and support animal nutrient requirement；With

About 25ppm to about 200ppm, it is preferable that the about capsaicin of 50ppm to about 125ppm。

Such as, the nutrition of the present invention can comprise with the cat food compositions of balance completely:

By weight about 0 to about 90%, it is preferable that the carbohydrate of about 5% to about 45%；

By weight about 5% to about 70%, it is preferable that the protein of about 20% to about 60%；

By weight about 2% to about 50%, it is preferable that the fat of about 5% to about 40%；

By weight about 0.1% to about 20%, it is preferable that the total dietary fiber of about 1% to about 11%；

By weight about 0 to about 15%, it is preferable that other nutrient of the vitamin of about 2% to about 8% and mineral, antioxidant and support animal nutrient requirement；With

About 25ppm to about 200ppm, it is preferable that the about capsaicin of 50ppm to about 125ppm。

As it has been described above, the compositions of the present invention can additionally comprise in soluble fiber, methionine, EPA and DHA any or multiple。Those skilled in the art are easy with determining respective appropriate amount without excessively experiment, for instance by carrying out bioavailability, heat stability and/or effect research according to the traditional method for preparing pet food composition。Such as, amount can be about 0.3% to about 7% soluble fiber, about 0.50% to about 1.50% methionine, about 0.10% to about 0.60%EPA and about 0.10% to about 0.50%DHA。

Purpose that the compositions of the present invention can additionally realize with the lossless present invention and the amount of effect are containing additive generally known in the art, for instance stabilizer and processing aid。Stabilizer can be added and improve the storage life of said composition with the effect by supplementary or that enhancing is for improving storage life physical method。The example of stabilizer is preservative, antioxidant, synergist and chelating agen, packaging gas, emulsifying agent, thickening agent, gellant and wetting agent。The example of emulsifying agent and thickening agent includes, but not limited to gelatin, cellulose ether, starch, starch ester, starch ether and modified starch。Other additive known in the art can be used for nutrition and palatability purpose, and its suitable amount can be readily determined by those of ordinary skill in the art。It is, for example possible to use this kind of additive of about at most 5 weight %。Additive can also include, but not limited to ferrum oxide, sodium chloride, potassium citrate, potassium chloride and other edible salts, flavoring agent, vitamin, mineral and coloring agent。

Although it is envisaged that the food of any denseness or water capacity, but the compositions of the present invention preferably can be such as wet or dry animal food compositions。The food that " wets " refers to sells and has about 70 to the food of about 90% water capacity in canned food or paper tinsel bag。" do " food and refer to have about 5 to the compositions of about 15% water capacity, and generally with comminutions or coarse particles manufacture。Such as United States Patent (USP) 6, described in 517,877, also contemplate the component being likely to comprise various denseness herein and be likely to be of the compositions of the component exceeding a kind of denseness, such as, the meat-like particle of soft Chewy and there is outer cereal component and the coarse grain of internal cream component。

Think the compositions of the classical production process manufacture present invention that those skilled in the art can be adopted to be familiar with herein。Any moment in the fabrication process can add Capsaicinoids, but, consider the volatilization character of these compounds, SC is answered to be avoided by any possible loss (such as, recording in Scoville device) of the activity in heat treatment manufacture process or " heat " and contact with any unnecessary of Capsaicinoids in the fabrication process。

As contemplated herein, the compositions of the present invention can with from about 25ppm to about 200ppm, it is preferable that from about 50ppm, more preferably comprise Capsaicinoids from the active principle of about 125ppm。It is apparent that the amount of Capsaicinoids high will not cause disadvantageous, unacceptable or harmful physical effects to the animal after ingesting。Disclosed in embodiment provided below, the compositions of the present invention comprises effectively prevention or treatment obesity in animals but animal ingestion both will not have been stoped without this animal is caused the Capsaicinoids of the amount of injurious effects on any health。

As it has been described above, another specific aspect of the present invention relates to by using the method that the compositions comprising one or more Capsaicinoids of effective dose is treated or prevented obesity in animals to animal。This Capsaicinoids is preferably capsaicin。The compositions and methods of the invention can be a part for overall loss of weight program, for instance, said composition and method can be co-administered with food more than regular exercise and restriction acquisition snacks, dining table or other house pet snack。In addition, owing to the compositions of the present invention makes this animal self regulation food ration, thinking that compositions disclosed herein is also particularly useful for is not the pet owner of health or the health status giving special heed to its house pet, for instance is likely to have the tendency of its house pet of overfeeding and/or is less inclined to take time and tempers the pet owner of its house pet。

Additionally, the present invention relates to Capsaicinoids or its active metabolite prevention or treatment obesity in animals compositions manufacture in and cause animal self regulation of ingesting compositions manufacture in purposes。For arbitrary purposes, in special embodiment, this Capsaicinoids is capsaicin or its active metabolite。In another embodiment, this Capsaicinoids is dihydrocapsaicin or its active metabolite。In another embodiment, what said composition can further include in soluble fiber, methionine, EPA and DHA is any or multiple。In one embodiment, said composition is wet pet foodstuffs。In another embodiment, said composition is dry pet food。In one embodiment, the animal treated is Canis animals。In another embodiment, the animal treated is felid。

Present invention additionally comprises any compositions disclosed herein purposes in the method for prevention or treatment obesity in animals, including effectively to prevent or to treat the amount of obesity of described animal to use the compositions of the present invention to described animal。Similarly, present invention additionally comprises any compositions disclosed herein purposes in causing the method for self regulation of ingesting of animal, including the amount of the self regulation of ingesting effectively to cause this animal to described animal applying said compositions。

Herein it is also contemplated that the present invention includes the external member of the compositions comprising the present invention。Except the compositions containing the present invention, this external member can farther include, for instance about the information of the obesity problem in companion animals with for having a mind to the instruction that improves the compositions including the feed present invention of the pet owner of the overall physical condition of its companion animals。This external member can further include the instruction of health and the instrument of monitoring its companion animals for pet owner, record material and instruction that house pet loses weight, such as but not limited to, for recording the traditional notebook lost weight of animal or daily record and being applicable to electronic installation or other device of this kind of data of electronic compilation, such as computer software。This external member can further include the exercise device of applicable house pet and pet owner, as ball, flying disc, flying saucer, with the belt of pedometer or similar device, or can be used as other article of a part for total loss of weight external member。Additionally, this external member can include snack low in calories or the snacks of the companion animals of the compositions of the feed present invention。This external member can be adapted at such as grocery store, pet shop or discount store and in veterinary clinic any form of commercial sale or retail。Traditional manufacture and the method packing external member as described herein can be used, and be that those skilled in the art are familiar with。

The further illustration present invention of the following example and be not meant to limit the present invention。

Embodiment

Embodiment 1

Can external change Canis animals gene expression material data base generate

Known basis that we are compiled is provided below data base's (referring to, for instance U.S.2006-0200320A1) of the material of the expression with the various Canis animals genes in above-described following method external change canid animal cell。

Use following canid animal cell system with two kinds of concentration check test material: CCL34 (kidney), CRL1430 (thymus), CCL183 (bone) (all available from theAmericanTissueCultureCollection) and CTAC (thyroid) (referring to, MeasurementofNKActivityinEffectorCellsPurifiedfromCanine PeripheralLymphocytes, VeterinaryImmunologyandImmunopathology, 35 (1993) 239-251)。In short, cultivate cell according to traditional method in OptiCell room, within second day, process by substances。It is used as tester with the solvent that the higher concentration in two kinds of concentration uses。Second day, obtain cell and carry out chromosome set inspection。

Use commercially available method, for instance RNAeasyMiniKit (Qiagen, Valencia, CA) separates RNA according to manufacturer's instruction and traditional method from Canis animals culture。The quality of the RNA that use RNA6000NanoAssay and Agilent2100Bioanalyzer analytical separation goes out。According to the manufacturer's instruction (version referring in November, 2003 provided in test kit guide (ReagentKitGuide), AgilentTechnologies) under following amendment, this program is carried out: in gel preparation course, it it not the aliquot that sieving gel is separated into each 65 microlitres, but deposit sieving gel is saved in original micro-centrifuge tube, and on demand by this 65 mul aliquots；While loading RNA6000NanoMarker, in the various kinds sample wells do not conform to sample, add 1 microlitre water (replacing RNA6000NanoMarker) without RNase-。When loading RNA6000Ladder and sample, heat makes sample and other 30 seconds of RNA6000Ladder degeneration (altogether 2.5 minutes) at 71 DEG C；About " StartingtheChipRun ", from proof list, select " EukaryoteTotalRNANano " option。

Use the gene expression of Affymetrix Canis animals gene chip Canine-1 and the Canine-2 instruction measurement processing cell line according to manufacturer and tester。For any given process, gene expression data is confirmed as " rise " or " downward "。The decision that gene is " rise " or " downward " changes based on multiple, and it calculates as the process intensity/tester intensity of each independent probes。If its value < 1/1.5 (analyses for across all 4 cell lines) or < 1/2 (analysis in cell line), the change of this multiple is considered to lower, if its > 1.5 (analyses for across all 4 cell lines) or > 2 (analysis in cell line), it is considered to raise。In addition, if according to software used its in the only one of the situation (process thing or tester) compared, be referred to as " existence " and in another, be referred to as " being absent from " or " small (marginal) " and multiple is changed significantly, then probe is considered notable to study further。The probe showing as reversely adjustment in the two processes is got rid of from analyze further。GeneSpring7.0 version (GS) software (AgilentCorporation) is used to analyze initial data and use R-Bioconductor (RB) freeware to confirm。The CEL file that the two software kit is all used for being generated by Affymetrix instrument calculates intensity of probe。Every probe be used alone R-Bioconductor and GeneSpring computed in software in the presence/absence of/small (Present/Absent/Marginal) conclusion and P-value。

Data analysis uses two schemes。First: " across cell line " and " in independent cell line "。In first scheme, selecting the gene of score, condition is that they are considered notable and is that all cells system has。" across cell line " produces the highest confidence data with least interference, and can provide the most probable clue about which gene of each composition influence。In alternative plan, only show, in processing at two according to the two software kit in independent cell line, those genes score that notable multiple changes。

Table 1A provided above is that it expresses the list of the Canis animals gene of external change under various compounds exist。Specifically, those the gene asterisks (*) changed by capsaicin indicate。

Embodiment 2

Internal Canis animals is ingested research

Based on the vitro data in embodiment 1, proceed as described below the internal Canis animals of the food that test comprises capsaicin and ingest research。

The body condition mark 10 overweight dogs higher than " 4 " (being likely in the scope that " 5 " are divided) are determined according to by traditional method in this study。To this 10 dog received control foods (table 1 and 2) 2 weeks。Record was ingested and recorded weekly body weight every day。Last 2 weeks feed phases, gathers blood sample analytical chemistry screening (see table 3) according to traditional method。After received control food two weeks, contain the novel therapeutic food (preparing according to traditional method) two weeks of " Fructus Capsici " (Kalsec, Kalamazoo, MI) with backward dog feed。Record was ingested and recorded weekly body weight every day。Last 2 weeks feed phases, gathers blood sample analytical chemistry screening (table 3)。

It is shown that the minimizing of ingesting that the dog of the food that absorption is containing Fructus Capsici is only；Therefore, the dog of the feed diet containing Fructus Capsici only alleviates 0.13 kilogram。Enjoyably, these dogs only also have the blood sugar level of reduction, serum urea nitrogen, alanine aminotransferase and alkali phosphatase (table 3-5)。

The component content of the table 1. treatment food containing Fructus Capsici

The nutrient content of the table 2. treatment food containing Fructus Capsici

The Chemical Screening data of table 3. received control food two weeks and the feed treatment food dog of two weeks

The weight data of table 4. received control food two weeks and the feed treatment food dog of two weeks

The data of ingesting of table 5. received control food two weeks and the feed treatment food dog of two weeks

Claims (9)

1. a compositions, said composition comprises Capsaicinoids or its active metabolite, and said composition is used for the self regulation of ingesting of animal,

The amount that wherein said Capsaicinoids exists is by active ingredient 25ppm to 200ppm,

Wherein said self regulation of ingesting includes induction satiety,

Wherein said animal is Canis animals or felid。

2. the compositions of claim 1, wherein said compositions can change the expression of one or more genes relevant to the restriction of the energy of animal。

3. the compositions of claim 1 or 2, wherein said Capsaicinoids is capsaicin or its active metabolite。

4. the compositions of any one of claim 1-3, wherein said Capsaicinoids is dihydrocapsaicin or its active metabolite。

5. the compositions of any one of claim 1-4, wherein said compositions additionally comprise selected from soluble fiber, methionine, EPA and DHA material。

6. the compositions of any one of claim 1-5, wherein said compositions is wet pet foodstuffs。

7. the compositions of any one of claim 1-5, wherein said compositions is dry pet food。

8. Capsaicinoids or its active metabolite purposes in preparation compositions, described compositions is for causing the self regulation of ingesting of animal, and the amount that wherein said Capsaicinoids exists is by active ingredient 25ppm to 200ppm,

Wherein said self regulation of ingesting includes induction satiety,

Wherein said animal is Canis animals or felid。

9. the purposes of claim 8, wherein said Capsaicinoids is capsaicin or dihydrocapsaicin or its active metabolite。