CN105681678A - Microscopic image imaging system and using method of microscopic image imaging system - Google Patents

Microscopic image imaging system and using method of microscopic image imaging system Download PDF

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Publication number
CN105681678A
CN105681678A CN201511002663.8A CN201511002663A CN105681678A CN 105681678 A CN105681678 A CN 105681678A CN 201511002663 A CN201511002663 A CN 201511002663A CN 105681678 A CN105681678 A CN 105681678A
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CN
China
Prior art keywords
described
imaging
imaging system
backlight
micro
Prior art date
Application number
CN201511002663.8A
Other languages
Chinese (zh)
Inventor
魏悦
陈文光
詹伟达
杨军
Original Assignee
上海美沃精密仪器有限公司
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Application filed by 上海美沃精密仪器有限公司 filed Critical 上海美沃精密仪器有限公司
Priority to CN201511002663.8A priority Critical patent/CN105681678A/en
Publication of CN105681678A publication Critical patent/CN105681678A/en

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Classifications

    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N5/00Details of television systems
    • H04N5/222Studio circuitry; Studio devices; Studio equipment ; Cameras comprising an electronic image sensor, e.g. digital cameras, video cameras, TV cameras, video cameras, camcorders, webcams, camera modules for embedding in other devices, e.g. mobile phones, computers or vehicles
    • H04N5/225Television cameras ; Cameras comprising an electronic image sensor, e.g. digital cameras, video cameras, camcorders, webcams, camera modules specially adapted for being embedded in other devices, e.g. mobile phones, computers or vehicles
    • H04N5/235Circuitry or methods for compensating for variation in the brightness of the object, e.g. based on electric image signals provided by an electronic image sensor
    • H04N5/2353Circuitry or methods for compensating for variation in the brightness of the object, e.g. based on electric image signals provided by an electronic image sensor by influencing the exposure time, e.g. shutter
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N5/00Details of television systems
    • H04N5/30Transforming light or analogous information into electric information
    • H04N5/335Transforming light or analogous information into electric information using solid-state image sensors [SSIS]
    • H04N5/351Control of the SSIS depending on the scene, e.g. brightness or motion in the scene
    • H04N5/355Control of the dynamic range
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N9/00Details of colour television systems
    • H04N9/04Picture signal generators
    • H04N9/045Picture signal generators using solid-state devices

Abstract

The invention provides a microscopic image imaging system and a using method of the microscopic image imaging system. The system comprises an objective table; an adjustable backlight device which is arranged on the objective table; an imaging lens which is arranged facing towards the objective table, wherein an image sensor is arranged on the imaging lens; a digital image processor which respectively communicates with the image sensor and the adjustable backlight device; and a display which communicates with the digital image processor. Compared with the prior art, the system and the method provided by the invention have advantages that 1, the problem of unsuitable backlight brightness, color and shape is solved; the contrast ratio of an observation object can be promoted through adjusting the backlight parameters; 2, the digitally imaged images are processed, calculated and fed back to control the backlight parameters; the problem of difficulty in finding a suitable backlight state through manually adjusting backlight parameters is avoided; 3, the backlight shape can be adjusted through an external computer; the backlight source shape can be customized; and 4, the photographed images can be recorded for exchange and share.

Description

Micro-imaging imaging system and using method thereof

Technical field

The present invention relates to a kind of formation method, belong to Medical Instruments microscopy field, particularly a kind of micro-imaging imaging system and using method thereof.

Background technology

In medical treatment detects; often have picture naked eyes under microscope and be difficult to observe clearly situation; the object observed is often because transparency and the reason of color and the contrast of background are only small, and when backlight color brightness is not as suitable, naked eyes are difficult to see in eyepiece. The present invention controls technology by automatic backlight and digital imaging technology solves this problem, automatically generates the image in the microscope ocular of high contrast, it is simple to user is observed on screen.

Summary of the invention

For defect of the prior art, it is an object of the invention to provide and a kind of solve the too low micro-imaging imaging system causing invisible problem of microscope object of observation contrast and using method thereof.

For solving above-mentioned technical problem, a kind of micro-imaging imaging system provided by the invention, including: object stage; Dimmable backlights device, described dimmable backlights device is arranged on described object stage; Imaging lens, described imaging lens is arranged towards described object stage, is provided with imageing sensor on described imaging lens; Digital Image Processor, described Digital Image Processor communicates with described imageing sensor and described dimmable backlights device respectively; Display, described display and described Digital Image Processor communication.

Preferably, described dimmable backlights device includes: backlight control module; Light source cell, light source cell is connected with described backlight control module; Projecting unit, described projecting unit is connected with described backlight control module, and described projecting unit is positioned in the light path of the light that described light source cell sends.

Preferably, described light source cell includes: close optical element; RGB three-color LED light source, described RGB three-color LED light source is connected with described backlight control module; Three light sources in wherein said RGB three-color LED light source are arranged around described conjunction optical element.

Preferably, described projecting unit includes: projection element, and described projection element is positioned in the light path of the light that described conjunction optical element sends;Projection lens and backlight, described projection lens and described backlight are successively set in the light path of the light emitted by described projection element.

Preferably, between described RGB three-color LED light source and described conjunction optical element, it is provided with condenser lens.

Preferably, in described RGB three-color LED light source: the wavelength of HONGGUANG is 600 nanometers~630 nanometers; The wavelength of green glow is 520 nanometers~540 nanometers; The wavelength of blue light is 460 nanometers~480 nanometers.

Preferably, described projection element is digital micromirror elements, LCD transflective liquid crystal element or LCOS reflective liquid crystal element.

Preferably, described Digital Image Processor includes intercoupling the image capture module, image processing module, image preservation module, the data transmission module that connect and controlling interface module.

The using method of a kind of micro-imaging imaging system, comprises the steps:

Step 1, dimmable backlights device works, and illuminates the object of observation on object stage;

Step 2, imageing sensor gathers picture, and image is processed;

Step 2A, if the contrast of treated image can reach the observational ability lower limit of human eye, then enters step 4;

Step 2B, if the contrast of treated image can not reach the observational ability lower limit of human eye, then enters step 3;

Step 3, regulates dimmable backlights device, again illuminates the object of observation on object stage, repeats step 2;

Step 4, is displayed the image processed by display device.

Preferably, in step 2, the operation to the process of image is calculate the mean flow rate of image by image processing module and regulate the time of exposure of imageing sensor.

Compared with prior art, beneficial effects of the present invention is as follows:

1, solve the backlight illumination inappropriate problem of color shape, can pass through to adjust backlighting parameters to promote the contrast of the object of observation;

2, the picture of digital image carries out processing calculating feedback control backlighting parameters, it is to avoid the problem that manual adjustment backlighting parameters is difficult to find that appropriate backlight state;

3, can by external computer regulated backlight shape, back light shape can carry out self-defined;

4, it is able to record that taken image, carries out exchanging and sharing.

Accompanying drawing explanation

By reading detailed description non-limiting example made with reference to the following drawings, the further feature purpose of the present invention and advantage will become more apparent upon.

Fig. 1 is micro-imaging imaging system structural representation of the present invention;

Fig. 2 is micro-imaging imaging system dimmable backlights device structural representation of the present invention.

In figure:

1-object stage 2-dimmable backlights device 3-imaging lens

4-Digital Image Processor 5-closes optical element 6-RGB three-color LED light source

7-projection element 8-projection lens 9-backlight

10-condenser lens 11-display 12-imageing sensor

13-backlight control module 14-computer

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is described in detail. Following example will assist in those skilled in the art and are further appreciated by the present invention, but do not limit the present invention in any form. It should be pointed out that, to those skilled in the art, without departing from the inventive concept of the premise, it is also possible to make some changes and improvements. These broadly fall into protection scope of the present invention.

A kind of micro-imaging imaging system provided by the invention, including: object stage 1, for placing the object needing to observe, including slide etc. Dimmable backlights device 2 is arranged on below object stage 1, and dimmable backlights device 2 is lighted, and with the color of acquiescence and brightness, illuminates the object of observation on object stage 1.Imaging lens 3 is towards object stage 1, for the object of observation is carried out imaging, then use CCD or cmos image sensor 12, by imaging lens 3, the object of observation is carried out imaging, the image collected is sent to Digital Image Processor 4 by high speed interface, is sent on display 11 after treatment and is shown (as shown in Figure 1).

Being made up of as in figure 2 it is shown, arrange RGB three-color LED light source 6, RGB three-color LED light source 6 in dimmable backlights device 2 three kinds of colors of red, green and blue, the wavelength of HONGGUANG is 600 nanometers~630 nanometers; The wavelength of green glow is 520 nanometers~540 nanometers; The wavelength of blue light is 460 nanometers~480 nanometers. And backlight control module 13 can receive outside (computer 14) control signal, for regulating the brightness of each color, it is finally synthesizing the light of different colours and brightness. The light that RGB three-color LED light source 6 sends first passes around condenser lens 10 to be polymerized, and is mixed by the light source of three kinds of colors then through closing optical element 5. Then pass through projection element 7 (digital micromirror elements, LCD transflective liquid crystal element or LCOS reflective liquid crystal element) and control imaging, finally by projection lens 8, the light of imaging is incident upon in imaging backlight 9, presents the image of given shape that needs show, color, brightness.

The object of observation is carried out imaging by imaging lens 3 by imageing sensor 12, the image collected is sent to Digital Image Processor 4 by high speed interface, image capture module in Digital Image Processor 4 and imageing sensor 12 are attached, the image being sent to is received gathering, the brightness of picture, contrast, object of observation shape are added up and are calculated by image processing module, carry out the contrast enhancement processing of picture simultaneously, by enhanced image by interfaces such as VGA/HDMI/DVI, display is on display 11. Meanwhile, the result calculated is fed back to imageing sensor 12 and dimmable backlights device 2 by image processing module, also needs to feed back to parameter and the data of dimmable backlights device 2 by generation after controlling interface module reception simultaneously. After completing calculating, by the data transmission module being connected with the control interface of dimmable backlights device 2 with the control interface of imageing sensor 12, image processing module is calculated the parameter feedback obtained and be sent to imageing sensor 12 and dimmable backlights device 2. The image generated preserves in module it addition, can also be stored in image.

Calculating and the process of image are specially and calculate picture mean flow rate by image processing module, and regulate the time of exposure of imageing sensor 12, make picture mean flow rate be on the intermediate value of dynamic range of sensor. Image processing module calculates whether the contrast in image can meet the observation ability of human eye. If human eye can not be observed, then control imageing sensor 12, again it is exposed imaging, and picture is constantly superimposed on the result of upper times exposure image, picture contrast is made numerically to be gradually increased, can allow, until obtaining, the contrast that human eye differentiates, and intercept out by this contrast from whole picture and show on display 11.

If control imageing sensor 12 still can not obtain is satisfied with picture, start to start the adjustment to backlight, Digital Image Processor 4 sends backlight and regulates signal, picture is shot by the light source using brightness, colourity, saturation different, often switching primary source change, and double counting, it is obtained in that enough contrasts until obtaining.

Certainly, for some object of observation, using the structured light of given shape reasonable to observe, at this moment can pass through computer 14 or other equipment, be input in equipment by shape information, backlight can present required given shape.

Above specific embodiments of the invention are described. It is to be appreciated that the invention is not limited in above-mentioned particular implementation, those skilled in the art can make a variety of changes within the scope of the claims or revise, and this has no effect on the flesh and blood of the present invention. When not conflicting, embodiments herein and the feature in embodiment can arbitrarily be mutually combined.

Claims (10)

1. a micro-imaging imaging system, it is characterised in that including:
Object stage;
Dimmable backlights device, described dimmable backlights device is arranged on described object stage;
Imaging lens, described imaging lens is arranged towards described object stage, is provided with imageing sensor on described imaging lens;
Digital Image Processor, described Digital Image Processor communicates with described imageing sensor and described dimmable backlights device respectively;
Display, described display and described Digital Image Processor communication.
2. micro-imaging imaging system according to claim 1, it is characterised in that described dimmable backlights device includes:
Backlight control module;
Light source cell, light source cell is connected with described backlight control module;
Projecting unit, described projecting unit is connected with described backlight control module, and described projecting unit is positioned in the light path of the light that described light source cell sends.
3. micro-imaging imaging system according to claim 2, it is characterised in that described light source cell includes:
Close optical element;
RGB three-color LED light source, described RGB three-color LED light source is connected with described backlight control module; Wherein
Three light sources in described RGB three-color LED light source are arranged around described conjunction optical element.
4. micro-imaging imaging system according to claim 3, it is characterised in that described projecting unit includes:
Projection element, described projection element is positioned in the light path of the light that described conjunction optical element sends;
Projection lens and backlight, described projection lens and described backlight are successively set in the light path of the light emitted by described projection element.
5. micro-imaging imaging system according to claim 3, it is characterised in that be provided with condenser lens between described RGB three-color LED light source and described conjunction optical element.
6. the micro-imaging imaging system according to claim 3 or 5, it is characterised in that in described RGB three-color LED light source: the wavelength of HONGGUANG is 600 nanometers~630 nanometers; The wavelength of green glow is 520 nanometers~540 nanometers; The wavelength of blue light is 460 nanometers~480 nanometers.
7. micro-imaging imaging system according to claim 4, it is characterised in that described projection element is digital micromirror elements, LCD transflective liquid crystal element or LCOS reflective liquid crystal element.
8. micro-imaging imaging system according to claim 1, it is characterised in that described Digital Image Processor includes intercoupling the image capture module, image processing module, image preservation module, the data transmission module that connect and controlling interface module.
9. the using method of a micro-imaging imaging system, it is characterised in that comprise the steps:
Step 1, dimmable backlights device works, and illuminates the object of observation on object stage;
Step 2, imageing sensor gathers picture, and image is processed;
Step 2A, if the contrast of treated image can reach the observational ability lower limit of human eye, then enters step 4;
Step 2B, if the contrast of treated image can not reach the observational ability lower limit of human eye, then enters step 3;
Step 3, regulates dimmable backlights device, again illuminates the object of observation on object stage, repeats step 2;
Step 4, is displayed the image processed by display device.
10. the using method of micro-imaging imaging system according to claim 9, it is characterised in that in step 2, the operation to the process of image is calculate the mean flow rate of image by image processing module and regulate the time of exposure of imageing sensor.
CN201511002663.8A 2015-12-28 2015-12-28 Microscopic image imaging system and using method of microscopic image imaging system CN105681678A (en)

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Application Number Priority Date Filing Date Title
CN201511002663.8A CN105681678A (en) 2015-12-28 2015-12-28 Microscopic image imaging system and using method of microscopic image imaging system

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101251658A (en) * 2008-03-12 2008-08-27 友达光电股份有限公司 Display quality testing apparatus and testing method
JP2008242022A (en) * 2007-03-27 2008-10-09 Tokyo Metropolitan Univ Polarizing microscopic observation method and polarizing microscope system
CN101553727A (en) * 2006-08-04 2009-10-07 伊康尼西斯公司 Image processing method for a microscope system
CN202599337U (en) * 2012-06-06 2012-12-12 沈阳同联集团高新技术有限公司 Micro stereo vision digital microscope
CN105025231A (en) * 2014-07-10 2015-11-04 深圳市得意自动化科技有限公司 Photographing method with projection light source and photographing device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101553727A (en) * 2006-08-04 2009-10-07 伊康尼西斯公司 Image processing method for a microscope system
JP2008242022A (en) * 2007-03-27 2008-10-09 Tokyo Metropolitan Univ Polarizing microscopic observation method and polarizing microscope system
CN101251658A (en) * 2008-03-12 2008-08-27 友达光电股份有限公司 Display quality testing apparatus and testing method
CN202599337U (en) * 2012-06-06 2012-12-12 沈阳同联集团高新技术有限公司 Micro stereo vision digital microscope
CN105025231A (en) * 2014-07-10 2015-11-04 深圳市得意自动化科技有限公司 Photographing method with projection light source and photographing device

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Application publication date: 20160615