CN105675741A - Method for determining content of effective components in hair growing pill - Google Patents
Method for determining content of effective components in hair growing pill Download PDFInfo
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- CN105675741A CN105675741A CN201511030761.2A CN201511030761A CN105675741A CN 105675741 A CN105675741 A CN 105675741A CN 201511030761 A CN201511030761 A CN 201511030761A CN 105675741 A CN105675741 A CN 105675741A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Abstract
The invention discloses a method for determining the content of effective components in a hair growing pill. The method adopts high-performance liquid chromatography to determine, and determination comprises determination of the content of Radix Polygonum Multiflorum Preparata and determination of the content of salt-processed Psoralea corylifolia. The method for determining the content of effective components in a hair growing pill improves and completes the quality standard of a hair growing pill traditional Chinese medicine preparation, and adopts the high-performance liquid chromatography to determine the respective content of Radix Polygonum Multiflorum Preparata and salt-processed Psoralea corylifolia, so the method has the advantages of high precision and sensitivity, good stability, simple and fast operation, high recovery rate, provision of experiment bases for safe and reasonable medication, and ensuring of the safety, homogeneousness, stability, effectiveness and controllability of the quality of the hair growing pill traditional Chinese medicine preparation.
Description
Technical field
The present invention relates to the quality controlling means of a kind of Chinese medicine preparation, it is specifically related to a kind of method measuring active constituent content in medicinal bolus with function of generating hair.
Background technology
At present, in life, alopecia brings a lot of puzzlement, especially adds stress and psychological pressure, and these retroactions have impact on the state of an illness of people, comprise alopecia seborrheica, infectious alopecia, anemicus alopecia, hereditary alopecia, neuroticism alopecia and alopecia areata are asthenia of qi and blood heat at the big multi-pathogenesis of interior alopecia, deficiency of the liver and kindey etc., for alopecia, existing a lot of hair tonic medicine on market, existing commercially available medicinal bolus with function of generating hair is by Radix Polygoni Multiflori Preparata, salt Psoralea corylifolia, the root of bidentate achyranthes, Radix Angelicae Sinensis, Poria cocos, wolfberry fruit, salt South Dodder Seed Chinese Dodder Seed, Glossy Privet Fruit, Yerbadetajo Herb, mulberry fruit, Semen Sesami Nigrum, Radix Rehmanniae Preparata, loranthus parasiticus, walnut kernel, Semen Astragali Complanati, Fructus Cnidii, Placenta Hominis, Rhizome of Fortune's Drynaria, the Radix Astragali, manufacture-yellow essence, shizandra berry, glossy ganoderma, glutinous rehmannia, Leafy twigs of Oriental Arborvitae, kuh-seng and hawthorn 26 taste medicine are prepared from, in clinical application for many years, its curative effect is indubitable, but the quality control for medicinal bolus with function of generating hair, current standards detection methods is too simple, bigger gap is required relative to what current traditional Chinese medicine quality controlled. therefore existing quality controlling means can not effectively control the quality of medicinal bolus with function of generating hair, thus affect production and the quality of product.
Summary of the invention
It is an object of the invention to overcome the shortcoming of prior art, a kind of method measuring active constituent content in medicinal bolus with function of generating hair is provided, the method precision and highly sensitive, good stability, simple to operate, rate of recovery height, it is ensured that the medicinal bolus with function of generating hair quality of the pharmaceutical preparations safe, equal one, stable, effective, controlled.
The object of the present invention is achieved through the following technical solutions: a kind of method measuring active constituent content in medicinal bolus with function of generating hair, comprises the mensuration of the content of Radix Polygoni Multiflori Preparata and the mensuration of salt Psoralea corylifolia content, and the mensuration of described Radix Polygoni Multiflori Preparata content comprises the following steps:
S1. chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent; Taking acetonitrile-water as moving phase, determined wavelength is 320nm, and theoretical stage number calculates by 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides peak should be not less than 3000;
S2. the preparation of reference substance solution: get 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance appropriate, accurately weighed, add Diluted Alcohol and make the solution of every 1ml containing 0.1mg, be reference substance solution;
S3. the preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus and shred, get 10g or medicinal bolus with function of generating hair water-honeyed pill 6g, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds Diluted Alcohol 50ml, weighed weight, supplies the weight of less loss with Diluted Alcohol after reflux, shake even after with filtering with microporous membrane, get filtrate, it is need testing solution;
S4. measure: difference is accurate draws reference substance solution and each 10 μ l of need testing solution, injects high performance liquid chromatograph and measures, and this product contains Radix Polygoni Multiflori Preparata with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides meter, the every 1g of water-honeyed pill must not less than 0.23mg; The every ball of big honeyed bolus must not less than 1.5mg;
The mensuration of described salt Psoralea corylifolia content comprises the following steps:
(1) chromatographic condition and system suitability experiment: being weighting agent taking octadecylsilane chemically bonded silica, take methanol-water as moving phase, determined wavelength is 246nm, theoretical stage number calculates by psoralene peak should be not less than 3000;
(2) preparation of reference substance solution: get psoralene, isopsoralen reference substance in right amount, accurately weighed, add methyl alcohol and make the mixing solutions of every 1ml containing 10 μ g, be reference substance solution;
(3) preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus and shred, get 5g or medicinal bolus with function of generating hair water-honeyed pill 3g, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds methyl alcohol 50ml, weighed weight, supersound process 30min, the weight of less loss is supplied with methyl alcohol, shake even after with filtering with microporous membrane, get filtrate, be need testing solution;
(4) measure: accurate absorption reference substance solution and each 10 μ l of need testing solution respectively, inject high performance liquid chromatograph to measure, this product is containing salt Psoralea corylifolia with the total amount of psoralen and isopsorapen, and the every 1g of water-honeyed pill must not less than 0.12mg, and the every ball of big honeyed bolus must not less than 0.76mg.
Further, the volume ratio of described acetonitrile and water is 1:5～7.
Further, the temperature of described reflux is 85～95 DEG C, and the time is 25～35min.
Further, the volume ratio of described first alcohol and water is 1:1.2～1.7.
Further, the condition of described supersound process is: power 400～600W, and frequency is 50～60KHz.
The present invention has the following advantages: a kind of method measuring active constituent content in medicinal bolus with function of generating hair provided by the invention, the quality standard of medicinal bolus with function of generating hair Chinese medicine preparation has been carried out raising, perfect, high performance liquid chromatography is adopted the Radix Polygoni Multiflori Preparata in composition and salt Psoralea corylifolia content to be measured, the method precision and highly sensitive, good stability, simple and efficient to handle, rate of recovery height, for its safe and reasonable medication provides experimental basis, it is ensured that medicinal bolus with function of generating hair Chinese medicine preparation quality safe, equal one, stable, effective, controlled.
Embodiment
Below in conjunction with embodiment, the present invention will be further described, and protection scope of the present invention is not limited to the following stated.
Embodiment 1: the preparation of medicinal bolus with function of generating hair
Preparation method: above 26 tastes, pulverizes into fine powder, sieves, mixed even. Every 100g powder adds refining honey 35～50g and appropriate water, general ball or ball processed, dry, makes water-honeyed pill; Or add refining honey 100～120g make big honeyed bolus, to obtain final product.
Embodiment 2: measuring active constituent content in medicinal bolus with function of generating hair, comprise the mensuration of the content of Radix Polygoni Multiflori Preparata and the mensuration of salt Psoralea corylifolia content, the mensuration of described Radix Polygoni Multiflori Preparata content comprises the following steps:
S1. chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent;Take acetonitrile-water as the volume ratio of moving phase, acetonitrile and water being 1:5, determined wavelength is 320nm, and theoretical stage number calculates by 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides peak should be not less than 3000;
S2. the preparation of reference substance solution: get 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance appropriate, accurately weighed, add Diluted Alcohol and make the solution of every 1ml containing 0.1mg, be reference substance solution;
S3. the preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus and get 10g and shred, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds Diluted Alcohol 50ml, weighed weight, supplies the weight of less loss with Diluted Alcohol after reflux, the temperature of reflux is 85 DEG C, time is 25min, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, be need testing solution;
S4. measure: difference is accurate draws reference substance solution and each 10 μ l of need testing solution, injects high performance liquid chromatograph and measures, and this product contains Radix Polygoni Multiflori Preparata with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides meter, the every ball of big honeyed bolus must not less than 1.5mg;
The mensuration of described salt Psoralea corylifolia content comprises the following steps:
(1) chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent, take methanol-water as moving phase, the volume ratio of first alcohol and water is 1:1.2, and determined wavelength is 246nm, and theoretical stage number calculates by psoralene peak should be not less than 3000;
(2) preparation of reference substance solution: get psoralene, isopsoralen reference substance in right amount, accurately weighed, add methyl alcohol and make the mixing solutions of every 1ml containing 10 μ g, be reference substance solution;
(3) preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus 5g and shred, grinds thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds methyl alcohol 50ml, weighed weight, supersound process 30min, the power 400W of supersound process, frequency is 50KHz, supplies the weight of less loss with methyl alcohol, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, it is need testing solution;
(4) measuring: accurate absorption reference substance solution and each 10 μ l of need testing solution respectively, inject high performance liquid chromatograph and measure, this product is containing salt Psoralea corylifolia with the total amount of psoralen and isopsorapen, and the every ball of big honeyed bolus must not less than 0.76mg.
Embodiment 3: measuring active constituent content in medicinal bolus with function of generating hair, comprise the mensuration of the content of Radix Polygoni Multiflori Preparata and the mensuration of salt Psoralea corylifolia content, the mensuration of described Radix Polygoni Multiflori Preparata content comprises the following steps:
S1. chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent; Take acetonitrile-water as the volume ratio of moving phase, acetonitrile and water being 1:7, determined wavelength is 320nm, and theoretical stage number calculates by 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides peak should be not less than 3000;
S2. the preparation of reference substance solution: get 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance appropriate, accurately weighed, add Diluted Alcohol and make the solution of every 1ml containing 0.1mg, be reference substance solution;
S3. the preparation of need testing solution: get medicinal bolus with function of generating hair water-honeyed pill 6g, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds Diluted Alcohol 50ml, weighed weight, supplies the weight of less loss with Diluted Alcohol after reflux, the temperature of reflux is 95 DEG C, time is 35min, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, be need testing solution;
S4. measure: difference is accurate draws reference substance solution and each 10 μ l of need testing solution, injects high performance liquid chromatograph and measures, and this product contains Radix Polygoni Multiflori Preparata with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides meter, the every 1g of water-honeyed pill must not less than 0.23mg;
The mensuration of described salt Psoralea corylifolia content comprises the following steps:
(1) chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent, take methanol-water as moving phase, the volume ratio of first alcohol and water is 1:1.7, and determined wavelength is 246nm, and theoretical stage number calculates by psoralene peak should be not less than 3000;
(2) preparation of reference substance solution: get psoralene, isopsoralen reference substance in right amount, accurately weighed, add methyl alcohol and make the mixing solutions of every 1ml containing 10 μ g, be reference substance solution;
(3) preparation of need testing solution: medicinal bolus with function of generating hair water-honeyed pill 3g, grinds thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds methyl alcohol 50ml, weighed weight, supersound process 30min, the power 600W of supersound process, frequency is 60KHz, supplies the weight of less loss with methyl alcohol, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, it is need testing solution;
(4) measuring: accurate absorption reference substance solution and each 10 μ l of need testing solution respectively, inject high performance liquid chromatograph and measure, this product is containing salt Psoralea corylifolia with the total amount of psoralen and isopsorapen, and the every 1g of water-honeyed pill must not less than 0.12mg.
Embodiment 4: measuring active constituent content in medicinal bolus with function of generating hair, comprise the mensuration of the content of Radix Polygoni Multiflori Preparata and the mensuration of salt Psoralea corylifolia content, the mensuration of described Radix Polygoni Multiflori Preparata content comprises the following steps:
S1. chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent; Take acetonitrile-water as the volume ratio of moving phase, acetonitrile and water being 1:6, determined wavelength is 320nm, and theoretical stage number calculates by 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides peak should be not less than 3000;
S2. the preparation of reference substance solution: get 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance appropriate, accurately weighed, add Diluted Alcohol and make the solution of every 1ml containing 0.1mg, be reference substance solution;
S3. the preparation of need testing solution: get 10g medicinal bolus with function of generating hair honeyed bolus and shred, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds Diluted Alcohol 50ml, weighed weight, supplies the weight of less loss with Diluted Alcohol after reflux, the temperature of reflux is 90 DEG C, time is 30min, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, be need testing solution;
S4. measure: difference is accurate draws reference substance solution and each 10 μ l of need testing solution, injects high performance liquid chromatograph and measures, and this product contains Radix Polygoni Multiflori Preparata with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides meter, the every ball of big honeyed bolus must not less than 1.5mg;
The mensuration of described salt Psoralea corylifolia content comprises the following steps:
(1) chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent, take methanol-water as moving phase, the volume ratio of first alcohol and water is 1:1.5, and determined wavelength is 246nm, and theoretical stage number calculates by psoralene peak should be not less than 3000;
(2) preparation of reference substance solution: get psoralene, isopsoralen reference substance in right amount, accurately weighed, add methyl alcohol and make the mixing solutions of every 1ml containing 10 μ g, be reference substance solution;
(3) preparation of need testing solution: get 5g medicinal bolus with function of generating hair honeyed bolus and shred, grinds thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds methyl alcohol 50ml, weighed weight, supersound process 30min, the power 500W of supersound process, frequency is 55KHz, supplies the weight of less loss with methyl alcohol, shake even after be the filtering with microporous membrane of 0.45 μm with aperture, get filtrate, it is need testing solution;
(4) measure: accurate absorption reference substance solution and each 10 μ l of need testing solution respectively, inject high performance liquid chromatograph to measure, this product is containing salt Psoralea corylifolia with the total amount of psoralen and isopsorapen, and the every 1g of water-honeyed pill must not less than 0.12mg, and the every ball of big honeyed bolus must not less than 0.76mg.
Embodiment 5:
1. experimental subjects: experimental subjects is the medicinal bolus with function of generating hair of following batch, is respectively that Jilin side is big 20060901, Jilin side is big 20060902, Jilin side is big 20070101, Hebei Anguo, nine sesame hall 070401, nine sesame hall 070402, nine sesame hall 071001, nine sesame halls 071002;
2. experimental technique: with embodiment 4, measures 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides content, often criticizes replicate(determination) 2 times;
3. experimental result: in table 1.
Table 1: 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides assay result in trial-product
As known from Table 1: nine batch sample 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides are 0.251mg/g containing lining average. In version pharmacopeia in 2010 regulation Radix Polygoni Multiflori Preparata 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides content must not be less than 0.70%, calculate finished product content limit with this and should be 0.255mg/g, consider the difference that each batch of rate of transform may exist, therefore fix tentatively as 0.23mg/g containing surveying limit.
Embodiment 6:
1. experimental subjects: experimental subjects is the medicinal bolus with function of generating hair of following batch, is respectively that Jilin side is big 20060901, Jilin side is big 20060902, Jilin side is big 20070101, Hebei Anguo, nine sesame hall 070401, nine sesame hall 070402, nine sesame hall 071001, nine sesame halls 071002;
2. experimental technique: with embodiment 4, measures the content of psoralene in trial-product;
3. experimental result: in table 2.
Table 2: psoralene assay result in trial-product
| Sample number into spectrum | Total amount (mg/g) |
| Jilin side big 20060901 | 0.226 |
| Jilin side big 20060902 | 0.217 |
| Jilin side big 20070101 | 0.183 |
| Jilin side big 080801 | 0.127 |
| Hebei Anguo | 0.185 |
| Nine sesame halls 070401 | 0.292 |
| Nine sesame halls 070402 | 0.273 |
| Nine sesame halls 071001 | 0.228 |
| Nine sesame halls 071002 | 0.235 |
Nine batch sample psoralenes, isopsoralen total amount are 0.218mg/g containing lining average. Psoralene in version pharmacopeia in 2010 regulation Psoralea corylifolia, isopsoralen total amount must not less than 0.70%, calculate finished product content limit with this and should be 0.128mg/g, consider the difference that each batch of rate of transform may exist, therefore fix tentatively as 0.12mg/g containing surveying limit.
Claims (5)
1. one kind measures the method for active constituent content in medicinal bolus with function of generating hair, it is characterised in that, comprise the mensuration of the content of Radix Polygoni Multiflori Preparata and the mensuration of salt Psoralea corylifolia content, the mensuration of described Radix Polygoni Multiflori Preparata content comprises the following steps:
S1. chromatographic condition and system suitability experiment: take octadecylsilane chemically bonded silica as weighting agent; Taking acetonitrile-water as moving phase, determined wavelength is 320nm, and theoretical stage number calculates by 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides peak should be not less than 3000;
S2. the preparation of reference substance solution: get 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides reference substance appropriate, accurately weighed, add Diluted Alcohol and make the solution of every 1ml containing 0.1mg, be reference substance solution;
S3. the preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus and shred, get 10g or medicinal bolus with function of generating hair water-honeyed pill 6g, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds Diluted Alcohol 50ml, weighed weight, supplies the weight of less loss with Diluted Alcohol after reflux, shake even after with filtering with microporous membrane, get filtrate, it is need testing solution;
S4. measure: difference is accurate draws reference substance solution and each 10 μ l of need testing solution, injects high performance liquid chromatograph and measures, and this product contains Radix Polygoni Multiflori Preparata with 2,3,5,4 '-tetrahydroxystilbene-2-O-β-D-Glucose glycosides meter, the every 1g of water-honeyed pill must not less than 0.23mg; The every ball of big honeyed bolus must not less than 1.5mg;
The mensuration of described salt Psoralea corylifolia content comprises the following steps:
(1) chromatographic condition and system suitability experiment: being weighting agent taking octadecylsilane chemically bonded silica, take methanol-water as moving phase, determined wavelength is 246nm, theoretical stage number calculates by psoralene peak should be not less than 3000;
(2) preparation of reference substance solution: get psoralene, isopsoralen reference substance in right amount, accurately weighed, add methyl alcohol and make the mixing solutions of every 1ml containing 10 μ g, be reference substance solution;
(3) preparation of need testing solution: get medicinal bolus with function of generating hair honeyed bolus and shred, get 5g or medicinal bolus with function of generating hair water-honeyed pill 3g, grind thin, accurately weighed, put in tool plug Erlenmeyer flask, precision adds methyl alcohol 50ml, weighed weight, supersound process 30min, the weight of less loss is supplied with methyl alcohol, shake even after with filtering with microporous membrane, get filtrate, be need testing solution;
(4) measure: accurate absorption reference substance solution and each 10 μ l of need testing solution respectively, inject high performance liquid chromatograph to measure, this product is containing salt Psoralea corylifolia with the total amount of psoralen and isopsorapen, and the every 1g of water-honeyed pill must not less than 0.12mg, and the every ball of big honeyed bolus must not less than 0.76mg.
2. a kind of method measuring active constituent content in medicinal bolus with function of generating hair as claimed in claim 1, it is characterised in that, the volume ratio of described acetonitrile and water is 1:5～7.
3. a kind of method measuring active constituent content in medicinal bolus with function of generating hair as claimed in claim 1, it is characterised in that, the temperature of described reflux is 85～95 DEG C, and the time is 25～35min.
4. a kind of method measuring active constituent content in medicinal bolus with function of generating hair as claimed in claim 1, it is characterised in that, the volume ratio of described first alcohol and water is 1:1.2～1.7.
5. a kind of method measuring active constituent content in medicinal bolus with function of generating hair as claimed in claim 1, it is characterised in that, the condition of described supersound process is: power 400～600W, and frequency is 50～60KHz.
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| CN112213414A (en) * | 2020-09-02 | 2021-01-12 | 广西南宁百会药业集团有限公司 | Method for constructing HPLC fingerprint of hair growing tablets |
| CN115684429A (en) * | 2022-11-17 | 2023-02-03 | 湖南易能生物医药有限公司 | Quality control method and application of traditional Chinese medicine composition containing rehmannia |
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| CN112213414A (en) * | 2020-09-02 | 2021-01-12 | 广西南宁百会药业集团有限公司 | Method for constructing HPLC fingerprint of hair growing tablets |
| CN112213414B (en) * | 2020-09-02 | 2022-07-01 | 广西南宁百会药业集团有限公司 | Method for constructing HPLC fingerprint of hair growing tablets |
| CN115684429A (en) * | 2022-11-17 | 2023-02-03 | 湖南易能生物医药有限公司 | Quality control method and application of traditional Chinese medicine composition containing rehmannia |
| CN115684429B (en) * | 2022-11-17 | 2023-07-04 | 湖南易能生物医药有限公司 | Quality control method and application of traditional Chinese medicine composition containing rehmannia |
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