CN105660394A - Strong seedling medium for improving saffron transplanting survival rate - Google Patents
Strong seedling medium for improving saffron transplanting survival rate Download PDFInfo
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- CN105660394A CN105660394A CN201610011346.0A CN201610011346A CN105660394A CN 105660394 A CN105660394 A CN 105660394A CN 201610011346 A CN201610011346 A CN 201610011346A CN 105660394 A CN105660394 A CN 105660394A
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- survival rate
- improving
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- transplanting survival
- stigma croci
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Belonging to the technical field of plant tissue culture, the invention provides a strong seedling medium for improving saffron transplanting survival rate. The strong seedling medium adopts an MS medium as the basic medium, and is added with 6-BA (6-benzyladenine), NAA (naphthalene acetic acid), 2-chloropropenyl trimethylammonium chloride, agar powder and sucrose. The medium provided by the invention can make saffron tissue culture seedlings grew robustly and can achieve a high transplanting survival rate.
Description
Technical field
The invention belongs to field of plant tissue culture technique, particularly to a kind of strong seedling culture base improving Stigma Croci transplanting survival rate.
Background technology
Stigma Croci is a kind of famous and precious Chinese herbal medicine, and owing to it is that stigma is used as medicine, yield is extremely low, simultaneously again because its resource is extremely limited, but of many uses, cause it expensive, it is described as " plant gold " always, thus the research of croceous Fast-propagation is of crucial importance.
Tissue culture is one of important method of Stigma Croci Fast-propagation, but the growth fraction of tissue cultured seedling is more weak in current technology, it is impossible to the environment acute variation that adaptation is transplanted and caused, cause a large amount of tissue cultured seedling dead, not only cause waste, also affect croceous Fast-propagation efficiency.
Summary of the invention
There are the problems referred to above based on prior art, the present invention provides a kind of strong seedling culture base improving Stigma Croci transplanting survival rate, it is culture medium based on MS culture medium, and add 6-BA(6-benzyladenine), NAA(naphthalene acetic acid), 2-Chloroallyl trimethyl ammonium chloride, agar powder and sucrose, culture medium provided by the invention has makes Stigma Croci tissue cultured seedling robust growth, the advantage that transplanting survival rate is high.
A kind of strong seedling culture base improving Stigma Croci transplanting survival rate, it is culture medium based on MS culture medium, and adds 6-BA(6-benzyladenine), NAA(naphthalene acetic acid), 2-Chloroallyl trimethyl ammonium chloride, agar powder and sucrose.
6-BA(6-benzyladenine) concentration be 0.1-0.5mg/L, NAA(naphthalene acetic acid) the concentration that concentration is 0.1-0.5mg/L, 2-Chloroallyl trimethyl ammonium chloride be 100-400mg/L, agar powder concentration be 5-8g/L, sucrose concentration be 30-90g/L.
6-BA(6-benzyladenine) concentration be 0.2-0.4mg/L, NAA(naphthalene acetic acid) the concentration that concentration is 0.2-0.4mg/L, 2-Chloroallyl trimethyl ammonium chloride be 150-250g/L, agar powder concentration be 6-7g/L, sucrose concentration be 40-70g/L.
The pH value of the described culture medium improving Stigma Croci callus induction rate is 5.5-6.5.
The pH value of the described culture medium improving Stigma Croci callus induction rate is 6.0.
The invention has the beneficial effects as follows: 2-Chloroallyl trimethyl ammonium chloride can make that plant stem is sturdy, blade is generous, leaf color is dark green, well developed root system, improve the resistance of plant, with the addition of 2-Chloroallyl trimethyl ammonium chloride in the medium, Stigma Croci tissue cultured seedling can be made more healthy and stronger, it is easily adapted to the environment of acute variation, thus improving croceous transplanting survival rate.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment one: preparation improves the strong seedling culture base of Stigma Croci transplanting survival rate.
The strong seedling culture based component improving Stigma Croci transplanting survival rate shown in MS medium component according to table 1 and consumption and table 2 and consumption, prepare the strong seedling culture base of raising Stigma Croci transplanting survival rate of the present invention.
Put down each composition and 6-BA(6-benzyladenine that weigh in MS culture medium with day), NAA(naphthalene acetic acid), 2-Chloroallyl trimethyl ammonium chloride, agar powder and sucrose, it is placed in triangular flask, add appropriate distilled water, stirring and dissolving, and regulate pH value to 6.0 with HCL and KOH, it is settled to 1000mL. Triangular flask is sealed, is placed in high-pressure sterilizing pot, in 121 DEG C of sterilizing 20min, be subsequently placed in superclean bench natural cooling, standby.
In culture medium subpackage to tissue culture flasks, will dry in the air cool when will be cooled to 50-60 DEG C, by tissue culture's bottle closure after its solidification, standby.
The composition of table 1MS culture medium and consumption.
Table 2 improves strong seedling culture based component and the consumption of Stigma Croci transplanting survival rate.
Embodiment two: improve the test of the strong seedling culture base of Stigma Croci transplanting survival rate.
The strong seedling culture base improving Stigma Croci transplanting survival rate is prepared by the method described in embodiment one, standby.
From root media growth selection normal health, without brownization, without vitrified Stigma Croci seedling, super clean workbench takes out Stigma Croci seedling, unnecessary blade is cut with aseptic operation cutter, then by Stigma Croci seedling inoculation in the strong seedling culture base improving Stigma Croci transplanting survival rate prepared above, 1-2 centimetre of interval between every strain seedling. Then Stigma Croci seedling and culture medium being put in growth cabinet and cultivate, condition of culture is: illumination/interlunation: 14/10 hour, and cultivation temperature is 23 DEG C.
After cultivating 20, it can be seen that Stigma Croci seedling leaf color is dark green, stem is sturdy, well developed root system, related for seedling tissue culture flasks is taken out growth cabinet, it is placed in and cultivates seedling exercising 2 days in booth, then uncap again seedling exercising 3 days, seedling is transplanted in cultivation matrix after terminating by seedling exercising from culture medium, adds up seedling percent after one week.
Using the seedling replanting survival rate of strong seedling culture base of raising Stigma Croci transplanting survival rate provided by the invention to 86.31%, the survival rate of comparison kind group is high.
Claims (5)
1. the strong seedling culture base improving Stigma Croci transplanting survival rate, it is characterised in that it is culture medium based on MS culture medium, and adds 6-BA(6-benzyladenine), NAA(naphthalene acetic acid), 2-Chloroallyl trimethyl ammonium chloride, agar powder and sucrose.
2. a kind of strong seedling culture base improving Stigma Croci transplanting survival rate according to claim 1, it is characterized in that, 6-BA(6-benzyladenine) concentration be 0.1-0.5mg/L, NAA(naphthalene acetic acid) the concentration that concentration is 0.1-0.5mg/L, 2-Chloroallyl trimethyl ammonium chloride be 100-400mg/L, agar powder concentration be 5-8g/L, sucrose concentration be 30-90g/L.
3. a kind of strong seedling culture base improving Stigma Croci transplanting survival rate according to claim 2, it is characterized in that, 6-BA(6-benzyladenine) concentration be 0.2-0.4mg/L, NAA(naphthalene acetic acid) the concentration that concentration is 0.2-0.4mg/L, 2-Chloroallyl trimethyl ammonium chloride be 150-250g/L, agar powder concentration be 6-7g/L, sucrose concentration be 40-70g/L.
4. according to one of them described a kind of strong seedling culture base improving Stigma Croci transplanting survival rate of claims 1 to 3, it is characterised in that the pH value of the described culture medium improving Stigma Croci callus induction rate is 5.5-6.5.
5. according to one of them described a kind of strong seedling culture base improving Stigma Croci transplanting survival rate of claims 1 to 3, it is characterised in that the pH value of the described culture medium improving Stigma Croci callus induction rate is 6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610011346.0A CN105660394A (en) | 2016-01-09 | 2016-01-09 | Strong seedling medium for improving saffron transplanting survival rate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610011346.0A CN105660394A (en) | 2016-01-09 | 2016-01-09 | Strong seedling medium for improving saffron transplanting survival rate |
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| CN105660394A true CN105660394A (en) | 2016-06-15 |
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| CN201610011346.0A Pending CN105660394A (en) | 2016-01-09 | 2016-01-09 | Strong seedling medium for improving saffron transplanting survival rate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568419A (en) * | 2020-08-13 | 2023-01-06 | 江苏宝德农业科技有限公司 | Rapid propagation liquid culture medium for potato tissue culture seedlings for field planting and rapid propagation method for tissue culture seedlings for field planting of virus-free miniature potatoes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1954668A (en) * | 2005-10-25 | 2007-05-02 | 中国科学院过程工程研究所 | Scale tissue culture quickly reproducing method of saffron crocus seedball (sprout) |
| CN101720667A (en) * | 2008-10-10 | 2010-06-09 | 上海华宇药业有限公司 | Tissue culture rapid propagation method for crocus |
-
2016
- 2016-01-09 CN CN201610011346.0A patent/CN105660394A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1954668A (en) * | 2005-10-25 | 2007-05-02 | 中国科学院过程工程研究所 | Scale tissue culture quickly reproducing method of saffron crocus seedball (sprout) |
| CN101720667A (en) * | 2008-10-10 | 2010-06-09 | 上海华宇药业有限公司 | Tissue culture rapid propagation method for crocus |
Non-Patent Citations (1)
| Title |
|---|
| 朱保华: "番红花（Crocus sativus L.）离体快繁及无病毒植株的培育", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115568419A (en) * | 2020-08-13 | 2023-01-06 | 江苏宝德农业科技有限公司 | Rapid propagation liquid culture medium for potato tissue culture seedlings for field planting and rapid propagation method for tissue culture seedlings for field planting of virus-free miniature potatoes |
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Application publication date: 20160615 |
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