CN105532463A - Proliferation medium for increasing saffron crocus bud proliferation rate - Google Patents
Proliferation medium for increasing saffron crocus bud proliferation rate Download PDFInfo
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- CN105532463A CN105532463A CN201610011322.5A CN201610011322A CN105532463A CN 105532463 A CN105532463 A CN 105532463A CN 201610011322 A CN201610011322 A CN 201610011322A CN 105532463 A CN105532463 A CN 105532463A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a proliferation medium for increasing the saffron crocus bud proliferation rate, and belongs to the technical field of plant tissue culture. An MS culture medium is used as a basic culture medium, and 6-BA(6-benzyladenine), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and saccharose are added. The proliferation medium for increasing the saffron crocus bud proliferation rate has the advantage of being high in bud proliferation rate.
Description
Technical field
The invention belongs to field of plant tissue culture technique, particularly a kind of proliferated culture medium improving safflower Shoot propagation rate.
Background technology
Safflower is a kind of famous and precious Chinese herbal medicine, and because it is that column cap is used as medicine, output is extremely low, simultaneously again because its resource is extremely limited, but of many uses, cause it expensive, be described as " plant gold ", so of crucial importance to croceous Fast-propagation research always.
Current safflower Fast-propagation mainly adopts tissue cultures, and safflower tissue cultures can use bulb evoked callus, induction buds sprouting again after callus proliferation, but callus Character instability; Can also be direct by bulb induction buds sprouting, but the rate of increase of bud is low, is difficult to the requirement meeting Fast-propagation.
Summary of the invention
There are the problems referred to above based on prior art, the invention provides a kind of proliferated culture medium improving safflower Shoot propagation rate, it is medium based on MS medium, and add 6-BA(6-benzyladenine), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and sucrose, the proliferated culture medium of raising safflower Shoot propagation rate provided by the invention has the high advantage of bud appreciation rate.
Improve a proliferated culture medium for safflower Shoot propagation rate, it is medium based on MS medium, and adds 6-BA(6-benzyladenine), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and sucrose.
6-BA(6-benzyladenine) concentration be 1-5mg/L, P-CPA(p-chlorophenoxyacetic acid) concentration be 0.5-2mg/L, the concentration of coconut water is 100-400mg/L, the concentration of agar powder is 5-8g/L, the concentration of sucrose is 30-90g/L.
6-BA(6-benzyladenine) concentration be 2-4mg/L, P-CPA(p-chlorophenoxyacetic acid) concentration be 0.75-1.25mg/L, the concentration of coconut water is 150-250mg/L, the concentration of agar powder is 6-7g/L, the concentration of sucrose is 40-70g/L.
The pH value of the medium of described raising safflower callus induction rate is 5.5-6.5.
The pH value of the medium of described raising safflower callus induction rate is 6.0.
The invention has the beneficial effects as follows: the coconut water in medium has a large amount of natural plants growth desired substances, micro substance needed for enough enzymes and plant growth can be provided, use P-CPA(p-chlorophenoxyacetic acid) replace conventional NAA(methyl α-naphthyl acetate) as growth hormone, croceous Shoot propagation can be promoted better, improve bud appreciation rate.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment one: prepare a kind of proliferated culture medium improving safflower Shoot propagation rate.
The proliferation culture medium formula of the raising safflower Shoot propagation rate according to the MS culture medium prescription shown in table 1 and table 2, prepares the proliferated culture medium of raising safflower Shoot propagation rate of the present invention.
Take each composition in MS medium and 6-BA(6-benzyladenine respectively with balance), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and sucrose, be placed in triangular flask, add appropriate purified water, stirring and dissolving, be settled to 1000mL, and use HCL or KOH adjust ph to 6.0.Triangular flask is sealed, is placed in high-pressure sterilizing pot, in 121 DEG C of sterilizing 21min, then in superclean bench, the proliferated culture medium improving safflower Shoot propagation rate is divided and is filled in tissue culture flasks, naturally cool, by tissue cultures bottle closure, for subsequent use.
The composition of table 1MS medium and consumption thereof.
Table 2 improves composition and the consumption thereof of the proliferated culture medium of safflower Shoot propagation rate.
Embodiment two: the test of the proliferated culture medium of raising safflower Shoot propagation rate of the present invention.
According to the medium preparation method described in embodiment one, preparation improves the proliferated culture medium of safflower Shoot propagation rate, for subsequent use.
From inducing culture, select not pollution, grow normally, there is no brownization and vitrified safranine bud, safranine bud is taken out in super-clean bench, with aseptic operation cutter, bud is separated into a strain strain simple bud, be inoculated in the proliferated culture medium of the above-mentioned raising safflower Shoot propagation rate prepared, spaced 1.5 centimetres of every strain bud.
After inoculation terminates, safranine bud and medium are placed in climatic cabinate to cultivate, condition of culture is as follows: illumination/interlunation: 14/10 hour, intensity of illumination is 2000lux, cultivation temperature is 23 DEG C, cultivate and add up Shoot propagation coefficient afterwards in 30 days, growth coefficient reaches 4.79, improves 2.61 compared with the growth coefficient of the safranine bud of control group, substantially increases safflower Shoot propagation speed.
Claims (5)
1. improve a proliferated culture medium for safflower Shoot propagation rate, it is characterized in that, it is medium based on MS medium, and adds 6-BA(6-benzyladenine), P-CPA(p-chlorophenoxyacetic acid), coconut water, agar powder and sucrose.
2. a kind of proliferated culture medium improving safflower Shoot propagation rate according to claim 1, it is characterized in that, 6-BA(6-benzyladenine) concentration be 1-5mg/L, P-CPA(p-chlorophenoxyacetic acid) concentration be 0.5-2mg/L, the concentration of coconut water is 100-400mg/L, the concentration of agar powder is 5-8g/L, the concentration of sucrose is 30-90g/L.
3. a kind of proliferated culture medium improving safflower Shoot propagation rate according to claim 2, it is characterized in that, 6-BA(6-benzyladenine) concentration be 2-4mg/L, P-CPA(p-chlorophenoxyacetic acid) concentration be 0.75-1.25mg/L, the concentration of coconut water is 150-250mg/L, the concentration of agar powder is 6-7g/L, the concentration of sucrose is 40-70g/L.
4., according to a kind of proliferated culture medium that improve safflower Shoot propagation rate of claims 1 to 3 described in one of them, it is characterized in that, the pH value of the medium of described raising safflower callus induction rate is 5.5-6.5.
5. a kind of proliferated culture medium improving safflower Shoot propagation rate according to claim 4, is characterized in that, the pH value of the medium of described raising safflower callus induction rate is 6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610011322.5A CN105532463A (en) | 2016-01-09 | 2016-01-09 | Proliferation medium for increasing saffron crocus bud proliferation rate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610011322.5A CN105532463A (en) | 2016-01-09 | 2016-01-09 | Proliferation medium for increasing saffron crocus bud proliferation rate |
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| CN105532463A true CN105532463A (en) | 2016-05-04 |
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| CN201610011322.5A Pending CN105532463A (en) | 2016-01-09 | 2016-01-09 | Proliferation medium for increasing saffron crocus bud proliferation rate |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134993A (en) * | 2016-06-27 | 2016-11-23 | 上海交通大学 | The production method of Stigma Croci detoxification kind ball |
| CN108684524A (en) * | 2018-05-16 | 2018-10-23 | 芜湖市三山区绿色食品产业协会 | The method for tissue culture of dendrobium candidum |
| CN113667684A (en) * | 2021-05-12 | 2021-11-19 | 中国烟草总公司郑州烟草研究院 | Tobacco NtIMK2 receptor protein kinase and application thereof in drought resistance |
| CN116458429A (en) * | 2023-04-28 | 2023-07-21 | 广西壮族自治区南宁良凤江国家森林公园 | Application and method for tissue culture propagation of sargentgloryvine stem seeds and embryo |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565172A (en) * | 2003-06-11 | 2005-01-19 | 中国科学院过程工程研究所 | Bacteria free saffron seedling growth method |
| CN1954668A (en) * | 2005-10-25 | 2007-05-02 | 中国科学院过程工程研究所 | Scale tissue culture quickly reproducing method of saffron crocus seedball (sprout) |
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2016
- 2016-01-09 CN CN201610011322.5A patent/CN105532463A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565172A (en) * | 2003-06-11 | 2005-01-19 | 中国科学院过程工程研究所 | Bacteria free saffron seedling growth method |
| CN1954668A (en) * | 2005-10-25 | 2007-05-02 | 中国科学院过程工程研究所 | Scale tissue culture quickly reproducing method of saffron crocus seedball (sprout) |
Non-Patent Citations (2)
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| 李军等: "我国藏红花离体快繁技术研究进展", 《中国园艺文摘》 * |
| 杨宝明等: "藏红花组织培养外植体选择及丛生芽诱导研究", 《农业科技通讯》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134993A (en) * | 2016-06-27 | 2016-11-23 | 上海交通大学 | The production method of Stigma Croci detoxification kind ball |
| CN108684524A (en) * | 2018-05-16 | 2018-10-23 | 芜湖市三山区绿色食品产业协会 | The method for tissue culture of dendrobium candidum |
| CN113667684A (en) * | 2021-05-12 | 2021-11-19 | 中国烟草总公司郑州烟草研究院 | Tobacco NtIMK2 receptor protein kinase and application thereof in drought resistance |
| CN113667684B (en) * | 2021-05-12 | 2023-06-27 | 中国烟草总公司郑州烟草研究院 | Tobacco NtIMK2 receptor protein kinase and application thereof in drought resistance |
| CN116458429A (en) * | 2023-04-28 | 2023-07-21 | 广西壮族自治区南宁良凤江国家森林公园 | Application and method for tissue culture propagation of sargentgloryvine stem seeds and embryo |
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