CN105655598B - A kind of method of fixation in situ anode of microbial fuel cell microorganism - Google Patents
A kind of method of fixation in situ anode of microbial fuel cell microorganism Download PDFInfo
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- CN105655598B CN105655598B CN201511034635.4A CN201511034635A CN105655598B CN 105655598 B CN105655598 B CN 105655598B CN 201511034635 A CN201511034635 A CN 201511034635A CN 105655598 B CN105655598 B CN 105655598B
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/88—Processes of manufacture
- H01M4/8878—Treatment steps after deposition of the catalytic active composition or after shaping of the electrode being free-standing body
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P70/00—Climate change mitigation technologies in the production process for final industrial or consumer products
- Y02P70/50—Manufacturing or production processes characterised by the final manufactured product
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Abstract
The present invention relates to a kind of method of fixation in situ anode of microbial fuel cell microorganism, belong to microbiological fuel cell field, flora is produced electricity by using Ago-Gel investment fixation in situ anode of microbial fuel cell, control concentration ratio, the lysigenous temperature and time of gel of Ago-Gel, so that the microbiological fuel cell of microbiological fuel cell performance after immobilization apparently higher than on-fixed, Ago-Gel investment fixation in situization are adapted to microbiological fuel cell to run steadily in the long term.
Description
Technical field
The invention belongs to microbiological fuel cell field, be on the Research foundation of existing microbiological fuel cell, it is determined that
It is a kind of to make simple, harmless to biology, the stable and cheap microbial immobilized side in situ suitable for microbiological fuel cell
Method.
Background technology
The energy is to maintain human survival and society to continue the material base and valuable source of harmonious development.According to statistics, now
Utilization of the world to the energy 86% comes from fossil fuel resource, such as oil and coal.The population size of rapid growth is given
The goods and materials such as our environment and the energy bring huge pressure, threaten the sustainable development of human civilization.At present, chemical fuel
Resource is faced with aggregate reserve wretched insufficiency, and exploitation link is complicated, and utilization ratio is not high, is produced after non-renewable and burning big
Measure the serious problems such as the Environmental waste such as dusty gas and residue.Obviously, the progress of the mankind, the development of society and the rapid development of economy add
The consumption of the fossil resources such as fast oil coal, the population that fossil fuel will be difficult to the development and high speed for supporting World Economics increase
It is long.International Energy Agency predicts, will increase by more than 50% to the year two thousand thirty global energy actual demand, and petroleum resources are estimated will be
Following exhaustion in 100 years or so.Therefore, more concerns are placed on how to reduce CO by people2Discharge and improve using energy source effect
In the research of rate, renewable and " neutral carbon " of new energy turns into everybody common recognition.
To solve the problems, such as fossil energy anxiety and energy greenization, the research of new energy of traditional fossil energy is being substituted just
Carry out extensively.In recent years, the research of solar energy system, air windmill and fuel cell also achieves the achievement to attract people's attention.Grind
Study carefully and show, biomass energy is a kind of very potential renewable resource, and important derived energy chemical raw material sources.To current
Untill, biomass energy has turned into the fourth-largest resource in the whole world, is only second to after coal, oil and natural gas, accounts for global total energy consumption
14%, but progressively showing with crisis in food, the biomass energy based on grain have occurred striving asking for grain with people
Topic, this has also promoted exploration of the people to biomass energy new sources.
It is microbe-derived abundant in environment, and many important nutrients, antibiotic, immunodepressant etc. once or
The important sources of secondary metabolites.It is well known that microorganism can produce the fuel such as alcohol, methane, hydrogen from organic matter, except this
Outside, organic matter can also be changed into electric energy by microorganism.Therefore, microbiological fuel cell provides reclaims electricity from debirs
The possibility of energy and renewable biomass, by extensive concern.As a solution of continuity of environment row development, microorganism
Fuel battery energy produces electric energy while pollutant is removed, and in the case where the current energy is increasingly in short supply, has important grind
Study carefully meaning.
The theoretical production capacity of microbiological fuel cell is higher, and according to Nernst equation, when using acetic acid as electron donor, oxygen is
During electron acceptor, the theoretical potential of anode of microbial fuel cell and negative electrode is respectively -0.3V (vs NHE) and 0.8V (vs
NHE), the theoretical voltage that produces between two electrodes thus calculated is 1.1V, still, because the loss on electrode potential is (as lived
Change polarization, concentration polarization and ohmic loss), the open-circuit voltage for the microbiological fuel cell that research institute observes is significantly less than at present
Perfect condition, the influence factor of the difference on potential also include electron donor, electron acceptor and inoculum etc..In addition, electrode material
Material, concentration of substrate, ionic strength, temperature, pH value and reactor configuration etc. may all influence MFC electricity generation performance.
Microbiological fuel cell technology has larger potential using value, and on the research of microbiological fuel cell
Also a series of progress is achieved, still, microbiological fuel cell is in the treatment of waste water also there is in place of some shortcomings, mainly
Including：(1) power output is relatively low, and the universal power output of microbiological fuel cell is less than 2W/m2, with conventional fuel cell 1W/
cm2Power output compared to power output when still having a larger gap, and actual waste water is handled with microbiological fuel cell then more
It is low, generally less than 0.1W/m2；(2) battery configuration of suitable microbiological fuel cell extension, two-chamber microbial fuel electricity be there is no
Pond needs PEM and various cathode electronics acceptors, expensive so that the cost of manufacture of battery is higher, and single chamber air
Cathode microbial fuel cell negative electrode makes complicated and expensive catalyst, is also unfavorable for the extension of microbiological fuel cell；(3)
The microbial activities of anode and caused output voltage are had a great influence by environment and operating condition, at present microbiological fuel cell
Generally run under conditions of 30 DEG C, pH neutrality and organic concentration relatively low (1gCOD/L), (have to the matrix for adding anode chamber
Machine thing, actual waste water etc.) require higher, if actual waste water is pre-processed can increase processing cost up to matrix requirement, no
Practical application and popularization beneficial to microbiological fuel cell.
Microbiological fuel cell technology is as a kind of biological effluent treatment technology, because the growth for producing electricity bacterium needs,
Need more to consider whether handled waste water produces toxic action to electricity production bacterium when handling waste water, should so as to limit it
With.And immobilized microorganism technology has the features such as mithridatism impact capacity is strong, insensitive to environmental change in the treatment of waste water,
It is combined with microbiological fuel cell technology can solve microbiological fuel cell processing waste water problem encountered.
The present invention is using immobilized microorganism technology as research object, in the Research foundation of existing microbiological fuel cell
On, using fixation in situ anode of microbial fuel cell microorganism, by Ago-Gel investment by its microbe
In the anode of fuel cell, by the electricity generation performance of test microbes fuel cell and gel stability etc., it is determined that suitable for micro-
The a kind of of biological fuel cell anode makes simple, harmless to biology, the stable and cheap microbiological fuel cell that is applied to
Microorganism immobilization method in situ.
The content of the invention
The purpose of the present invention be by Ago-Gel investment fixation in situ anode of microbial fuel cell microorganism,
A kind of method of fixation in situ anode of microbial fuel cell microorganism is provided.
The present invention solves the technical problem of electricity-producing microorganism fixation in situ is realized, reach microbiological fuel cell
While processing high-enriched organics can be stablized, the purpose of output electricity production is efficiently realized.
In order to realize the purpose of the present invention, the technical solution adopted by the present invention is：
A kind of method of fixation in situ anode of microbial fuel cell microorganism, comprises the following steps：
(1) microbe fuel cell inoculation municipal wastewater treatment plant is intake, and adds the matrix such as sodium acetate, and anode richness is realized after startup
Collect the process of electricity-producing microorganism；
(2) weigh certain mass agarose be placed in phosphate buffer solution (8g/L sodium chloride, 0.2g/L potassium chloride,
1.44g/L disodium hydrogen phosphates, 0.24g/L potassium dihydrogen phosphates, regulation pH is in 7.4), controls volume ratio 1: 1~10: 1000, added
Heat is to being completely dissolved；
(3) agarose solution of dissolving is poured into culture dish, by microbiological fuel cell under the conditions of 30~50 DEG C
Anode is immersed in agarose solution；
(4) the formation time for controlling Ago-Gel is 10~100 minutes, clean with a large amount of deionized water rinsings, is obtained
Agarose immobilized microorganism fuel cell electricity-producing microorganism anode；
(5) the good anode of immobilization is fitted into reactor and run, determine the change of electricity generation performance.
A kind of the advantages of method of fixation in situ anode of microbial fuel cell microorganism provided by the present invention, is：
Ago-Gel moderate strength and the normal electricity production for not influenceing microbiological fuel cell, it is to be adapted to microbiological fuel cell to transport for a long time
Capable microbe immobilizing material and method.
Brief description of the drawings
Accompanying drawing 1 is the electricity production curve synoptic diagram of agarose immobilization and unlocked microbiological fuel cell.
Accompanying drawing 2 is agarose immobilization and unlocked microbiological fuel cell power density and electricity under different substrates concentration
Current density curve synoptic diagram.
Embodiment
The technical scheme in the embodiment of the present invention will clearly and completely be illustrated below.
A kind of method of fixation in situ anode of microbial fuel cell microorganism, comprises the following steps：
(1) microbe fuel cell inoculation municipal wastewater treatment plant is intake, and adds the matrix such as sodium acetate, and anode richness is realized after startup
Collect the process of electricity-producing microorganism；
(2) weigh certain mass agarose be placed in phosphate buffer solution (8g/L sodium chloride, 0.2g/L potassium chloride,
1.44g/L disodium hydrogen phosphates, 0.24g/L potassium dihydrogen phosphates, regulation pH is in 7.4), controls volume ratio 1: 1~10: 1000, added
Heat is to being completely dissolved；
(3) agarose solution of dissolving is poured into culture dish, by microbiological fuel cell under the conditions of 30~50 DEG C
Anode is immersed in agarose solution；
(4) the formation time for controlling Ago-Gel is 10~100 minutes, clean with a large amount of deionized water rinsings, is obtained
Agarose immobilized microorganism fuel cell electricity-producing microorganism anode；
(5) the good anode of immobilization is fitted into reactor and run, determine the change of electricity generation performance.
According to experiment show, by the electricity-producing microorganism anode after agarose immobilization, using sodium acetate matrix as
In the microbiological fuel cell of substrate can retention property it is stable, with the increase in electricity production cycle, the maximum of microbiological fuel cell
Output voltage brings up to 0.4~0.6V.
According to experiment show, Ago-Gel moderate strength and not shadow in the longtime running of microbiological fuel cell
The normal electricity production of microbiological fuel cell is rung, is microbe immobilizing material and the side for being adapted to microbiological fuel cell longtime running
Method.
Claims (3)
- A kind of 1. method of fixation in situ anode of microbial fuel cell microorganism, it is characterised in that comprise the following steps：(1) microbe fuel cell inoculation municipal wastewater treatment plant is intake, and adds sodium acetate matrix, and anode enrichment electricity production is realized after startup The process of microorganism；(2) weigh agarose to be placed in phosphate buffer solution, control the agarose and the mass body of phosphate buffer solution Product ratio 1:1~10:1000, it is heated to being completely dissolved；The composition of the phosphate buffer solution is：8g/L sodium chloride, 0.2g/L Potassium chloride, 1.44g/L disodium hydrogen phosphates, 0.24g/L potassium dihydrogen phosphates；The pH of the phosphate buffer solution is 7.4；(3) agarose solution of dissolving is poured into culture dish, by the anode of microbiological fuel cell under the conditions of 30~50 DEG C Immerse in agarose solution；(4) the formation time for controlling Ago-Gel is 10~100 minutes, clean with a large amount of deionized water rinsings, obtains agar Glycopexis microbiological fuel cell electricity-producing microorganism anode；(5) the good anode of immobilization is fitted into reactor and run, determine the change of electricity generation performance.
- 2. a kind of method of fixation in situ anode of microbial fuel cell microorganism according to claim 1, its feature It is：By the electricity-producing microorganism anode after agarose immobilization, using sodium acetate matrix as in the microbiological fuel cell of substrate Can retention property it is stable, with the increase in electricity production cycle, the maximum output voltage of microbiological fuel cell brings up to 0.4~ 0.6 volt.
- 3. a kind of method of fixation in situ anode of microbial fuel cell microorganism according to claim 1, its feature It is：Electricity-producing microorganism anode after agarose immobilization, when using 15g/L sodium acetates for matrix, maximum output voltage reaches 450mV。
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7767323B1 (en) * | 2006-12-19 | 2010-08-03 | University Of South Florida | Microbial fuel cell |
| CN103380527A (en) * | 2011-02-24 | 2013-10-30 | 索尼公司 | Microbial fuel cell, fuel and microbes for said fuel cell, bioreactor and biosensor |
| CN104611262A (en) * | 2015-01-30 | 2015-05-13 | 河北大学 | Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells |
| CN104716336A (en) * | 2015-03-25 | 2015-06-17 | 江西师范大学 | Hydrogel microbial electrode and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7767323B1 (en) * | 2006-12-19 | 2010-08-03 | University Of South Florida | Microbial fuel cell |
| CN103380527A (en) * | 2011-02-24 | 2013-10-30 | 索尼公司 | Microbial fuel cell, fuel and microbes for said fuel cell, bioreactor and biosensor |
| CN104611262A (en) * | 2015-01-30 | 2015-05-13 | 河北大学 | Electricity-producing bacterium capable of degrading cellulose and application of electricity producing bacterium in fuel cells |
| CN104716336A (en) * | 2015-03-25 | 2015-06-17 | 江西师范大学 | Hydrogel microbial electrode and preparation method thereof |
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