CN105647831B - Promote the method for flocculability bacterial growth with spinning roller worm culture solution - Google Patents
Promote the method for flocculability bacterial growth with spinning roller worm culture solution Download PDFInfo
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Abstract
The present invention relates to a kind of methods for promoting flocculability bacterial growth with spinning roller worm culture solution.The present invention obtains spinning roller worm culture solution as follows: 1) being the culture solution culture spinning roller worm of bait to flour；2) when spinning roller worm density reaches 100 ~ 500ind/mL, with 300 mesh nylon net filter spinning roller worm culture solutions, spinning roller worm is removed from spinning roller worm culture solution；3) filtrate obtained by step 2 is separated with ultrafiltration membrance filter again, further removing tiny particles contents, the thus obtained filtrates such as the flour in filtrate is spinning roller worm culture solution of the present invention.Spinning roller worm culture solution of the present invention is 0.05% ~ 1% as the usage amount of flocculability bacterial growth promoter.The method that the present invention promotes flocculability bacterial growth using spinning roller worm culture solution, has the characteristics that ecological, easy to use, effect is obvious.
Description
Technical field
The present invention relates to a kind of methods for promoting flocculability bacterial growth with spinning roller worm culture solution.
Background technique
Activated sludge process is most widely used method in dirty water and wastewater treatment.The flocculability of activated sludge is to influence to live
One of operation stability, treatment effeciency and the key factor of effluent quality of property sludge treating system.The flocculability of activated sludge
Better, the performance of active sludge processing system is better.The flocculability of activated sludge depends on the quantity and work of flocculability bacterium
Property, increase the quantity of flocculability bacterium or improves the active raising for being conducive to activated sludge flocculability of flocculability bacterium.
The method for improving activated sludge flocculability is segmented into two major classes: chemical method and biological methods.Chemical method is to pass through
Inorganic flocculating agent or organic flocculant are added, the volume of activated sludge is increased or increases the density of activated sludge, makes activated sludge
Flocculability be improved.The side effect of this method is that salt content in water is made to increase, easily form secondary pollution, cause flocculability
The problems such as bacterial activity declines.Biological methods are that the flocculation of the growth or raising flocculability bacterium by promoting flocculability bacterium is living
Property improves the flocculability of activated sludge.Chinese invention patent (CN 102816797) is disclosed to be increased by using L-cysteine
The method of strong light zymogenous bacteria excrement red pseudomonas flocculating property, but there is use object specific and L-cysteine for this method
Price it is higher the problems such as.
Spinning roller worm is a kind of micro-metazoa common in activated sludge, and Bdelloid rotifers section is belonged on taxology.?
In activated sludge process when the Bdelloid rotifers quantity such as spinning roller worm is more, it may appear that the quantity of flocculability bacterium is obvious in activated sludge
The obvious good phenomenon of more, activated sludge flocculability, reason and spinning roller worm can secrete containing promotion flocculability bacterial growth
Active material it is related.Therefore, it can be secreted by being added into activated sludge or flocculability bacteria culture media containing spinning roller worm
The spinning roller worm culture solution of active material promote the growth of flocculability bacterium.
Summary of the invention
The purpose of the present invention is to provide a kind of method for promoting flocculability bacterial growth using spinning roller worm culture solution, the party
Method improves the growth of flocculability bacterium using spinning roller worm culture solution, easy to operate, ecological, easy to use, can increase flocculation
Property bacterium density, improve the flocculability of bacterium, be conducive to be widely applied, have preferable economic benefits.
A method of promoting flocculability bacterial growth using spinning roller worm culture solution, it is characterised in that the specific step of this method
Suddenly are as follows:
A. spinning roller worm is placed in spinning roller worm culture solution and is bred, when the density of spinning roller worm reaches 100 ~ 500ind/mL
When, spinning roller worm is removed with 300 mesh nylon net filters；
B. by the obtained culture solution ultrafiltration membrance filter of step a, the tiny particles content in filtrate is further removed；
C. the flocculability bacterium that inclined-plane saves is seeded in 100mL LB culture medium with oese, is subsequently placed in 120rpm
Shaking table on, in 30 DEG C continuous culture 24 hours, centrifugal treating culture solution is washed with deionized, with sterile water that bacterium is dense
Degree is adjusted to OD600Value is 0.6 ~ 0.8, this solution is bacterium bacteria suspension；
D. bacterium bacteria suspension that step c is obtained is added to 5% ~ 10% additive amount of simulated domestic wastewater volume sterilized
In the simulated domestic wastewater of bacterium to get arrive bacteria culture media；
E. spinning roller worm culture solution obtained by step b is added to step with 0.05% ~ 1% additive amount of bacteria culture media volume
It in the resulting bacteria culture media of d, is subsequently placed on the shaking table of 120rpm, cultivates 48 hours continuously in 30 DEG C to get to containing thin
The culture solution of bacterium floccule body.
Above-mentioned flocculability bacterium are as follows: vesicle shortwave monad, bacillus cereus, bacillus thuringiensis, withered grass bud
Spore bacillus, Acinetobacter bauamnnii one or more.
Above-mentioned simulated domestic wastewater formula are as follows: 0.17g glucose, 0.16g starch, 0.233g sodium acetate, 0.025g chlorine
Change ammonium, 0.158g peptone, 0.04g beef extract, 0.0284g ammonium sulfate, 0.07g potassium dihydrogen phosphate and 0.06g sodium carbonate,
And 1000mL deionized water.
Remarkable advantage of the invention is: 1) present invention promotes the side of the growth of flocculability bacterium using spinning roller worm culture solution
Method has the characteristics that green, environmentally friendly, without secondary pollution, easy to use.2) under the action of spinning roller worm culture solution, flocculability is thin
The growth of bacterium is promoted, and the more biological flocculants of flocculability bacterial secretory can be made.3) spinning roller worm culture solution can promote more
The flocculability bacterium of type grows, applied widely, can be effectively applied in activated sludge process.
Detailed description of the invention
Fig. 1 is the dry cell weight size of bacillus cereus when adding spinning roller worm culture solution and not adding spinning roller worm culture solution
Compare figure.
Fig. 2 is that the dry cell weight of vesicle shortwave monad when adding spinning roller worm culture solution and not adding spinning roller worm culture solution is big
It is small relatively to scheme.
Fig. 3 is that the dry cell weight of bacillus thuringiensis when adding spinning roller worm culture solution and not adding spinning roller worm culture solution is big
It is small relatively to scheme.
Fig. 4 is that the dry cell weight size of mixed cell when adding spinning roller worm culture solution and not adding spinning roller worm culture solution compares
Figure.
Specific embodiment
It elaborates below in conjunction with attached drawing to the embodiment of the present invention.
Embodiment 1
Using spinning roller worm culture solution promote bacillus cereus (Bacillus cereus) growth method, by following step
It is rapid to carry out:
(1) the bacteria suspension preparation of bacillus cereus: the bacillus cereus being stored in medium slant is inoculated with
Ring is seeded in LB culture medium, is subsequently placed on the shaking table of 120rpm, is continuously cultivated 24 hours in 30 DEG C, centrifugal treating culture
Liquid is washed with deionized, and is thus centrifuged repeatedly, after deionized water washing three times, is adjusted to bacterial concentration with sterile water
OD600Value 0.6.This solution is the bacteria suspension of bacillus cereus.
(2) simulated domestic wastewater is prepared: weighing 0.17g glucose, 0.16g starch, 0.233g sodium acetate, 0.025g chlorination
Ammonium, 0.158g peptone, 0.04g beef extract, 0.0284g ammonium sulfate, 0.07g potassium dihydrogen phosphate and 0.06g sodium carbonate, it is molten
Enter 1000mL deionized water.
(3) prepared by spinning roller worm culture solution: the spinning roller worm in spinning roller worm culture solution is separated with 300 mesh nylon net filters, with nothing
Bacterium tap water rinses the spinning roller worm being trapped on nylon wire 5 times repeatedly.Spinning roller worm is transferred to sterile tap water from nylon wire
In, so that spinning roller worm density is reached 450 ind/mL, be subsequently placed on the shaking table of 120rpm, is cultivated at 30 DEG C for 24 hours, use nylon wire
Broth filtrate, and the tiny particles contents such as bacterium are removed with 0.22 μm of membrane filtration, filtrate is collected, filtrate is settled to
100mL.This filtrate is spinning roller worm culture solution.
(4) experimental group prepares: two experimental groups of setting, wherein one group is control group, another group adds for spinning roller worm culture solution
Add group.Two experimental groups use 250mL conical beaker be experiment container, the bacterium obtained using step (1) as experimental subjects, with
The simulated domestic wastewater that step (2) obtains is inoculum.Add 90 mL's respectively in the conical beaker of two experimental groups
Simulated domestic wastewater, the bacteria suspension of 10mL.Add the spinning roller worm culture solution of 0.5mL again in the conical beaker of addition group.
(5) conical beaker of two experimental groups obtained by step (4) Bacteria Culture: is placed in the shaking table of 120rpm
On, it is continuously cultivated 48 hours in 30 DEG C.
(6) using the bacterium of centrifugal process collection step (5) culture, two then dry cell weight: are obtained by dry, weighing
The bacterial cell dry weight (Fig. 1) of experimental group.
(7) spinning roller worm culture solution promotes the growth result of bacillus cereus: as shown in Figure 1, adding in inoculum
The addition group of spinning roller worm culture solution, compared with the control group for being not added with spinning roller worm culture solution, bacillus cereus dry cell weight
60% is increased, shows that spinning roller worm culture solution has apparent facilitation effect to the growth of bacillus cereus.
Embodiment 2
Using spinning roller worm culture solution promote vesicle shortwave monad (Brevundimonasvesicularis) growth side
Method sequentially includes the following steps:
1. prepared by the bacteria suspension of vesicle shortwave monad: in addition to strain used is vesicle shortwave monad, the system of bacteria suspension
Standby method is identical as step (1) in embodiment 1.
2. simulated domestic wastewater is prepared: step is identical as step (2) in embodiment 1.
3. prepared by spinning roller worm culture solution:
(1) spinning roller worm is inoculated into using flour as in the distilled water of bait or deionized water.
(2) spinning roller worm is cultivated under conditions of shading, shaking speed 120rpm, 30 DEG C.
(3) it when the density after the breeding of spinning roller worm reaches 100 ~ 500ind/mL, is trained with 300 mesh nylon net filter spinning roller worms
Nutrient solution removes spinning roller worm from spinning roller worm culture solution.
(4) filtrate obtained again with ultrafiltration membrance filter by step (3) further removes the fine particles such as the flour in filtrate
Object, thus obtained filtrate are spinning roller worm culture solution of the present invention.
4. promoting bacterial growth Preparatory work of experiment: in addition to the bacteria suspension that bacteria suspension used is vesicle shortwave monad, step is equal
It is identical as step (4) in embodiment 1.
5. Bacteria Culture: step is identical as step (5) in embodiment 1.
6. dry cell weight: step is identical as step (6) in embodiment 1.
7. the growth result of spinning roller worm culture solution promotion vesicle shortwave monad: as shown in Figure 2, being added in inoculum
The addition group of spinning roller worm culture solution, compared with the control group for being not added with spinning roller worm culture solution, vesicle shortwave Pseudomonad cell is dry
29.7% is increased again, shows that spinning roller worm culture solution has preferable facilitation effect to the growth of vesicle shortwave monad.
Embodiment 3
Using spinning roller worm culture solution promote bacillus thuringiensis (Bacillus thuringiensis) growth method,
It sequentially includes the following steps:
1. prepared by the bacteria suspension of bacillus thuringiensis: in addition to strain used is bacillus thuringiensis, the system of bacteria suspension
Standby method is identical as step (1) in embodiment 1.
2. simulated domestic wastewater is prepared: step is identical as step (2) in embodiment 1.
3. prepared by spinning roller worm culture solution:
(1) spinning roller worm is inoculated into using flour as in the distilled water of bait or deionized water.
(2) spinning roller worm is cultivated under conditions of shading, shaking speed 120rpm, 30 DEG C.
(3) it when the density after the breeding of spinning roller worm reaches 100 ~ 500ind/mL, is trained with 300 mesh nylon net filter spinning roller worms
Nutrient solution removes spinning roller worm from spinning roller worm culture solution.
(4) filtrate obtained again with ultrafiltration membrance filter by step (3) further removes the fine particles such as the flour in filtrate
Object, thus obtained filtrate are spinning roller worm culture solution of the present invention.
4. promoting bacterial growth Preparatory work of experiment: in addition to the bacteria suspension that bacteria suspension used is bacillus thuringiensis, step is equal
It is identical as step (4) in embodiment 1.
7. Bacteria Culture: step is identical as step (5) in embodiment 1.
8. dry cell weight: step is identical as step (6) in embodiment 1.
7. the growth result of spinning roller worm culture solution promotion bacillus thuringiensis: as shown in Figure 2, being added in inoculum
The addition group of spinning roller worm culture solution, compared with the control group for being not added with spinning roller worm culture solution, vesicle shortwave Pseudomonad cell is dry
67.9% is increased again, shows that spinning roller worm culture solution has preferable facilitation effect to the growth of bacillus thuringiensis.
Embodiment 3
Using spinning roller worm culture solution promote by bacillus cereus (Bacillus cereus), vesicle shortwave monad
(Brevundimonasvesicularis), bacillus thuringiensis (Bacillus thuringiensis) these three bacteriums are mixed
The method for the Mixed Microbes growth being combined, sequentially includes the following steps:
(1) Mixed Microbes bacteria suspension preparation: to bacillus cereus, vesicle shortwave monad, bacillus thuringiensis this
Three kinds of bacteriums are prepared into the bacteria suspension of single bacterium according to the step (1) in embodiment 1 respectively.By the bacteria suspension of three kinds of bacterium with 1:1:1
Ratio be mixed into the bacteria suspensions of Mixed Microbes.
(2) simulated domestic wastewater is prepared: step is identical as step (2) in embodiment 1.
(3) prepared by spinning roller worm culture solution: step is identical as step (3) in embodiment 1.
(4) promote bacterial growth Preparatory work of experiment: in addition to the bacteria suspension that bacteria suspension used is Mixed Microbes, step is and embodiment
Step (4) in 1 is identical.
(5) Bacteria Culture: step is identical as step (5) in embodiment 1.
(6) dry cell weight: step is identical as step (6) in embodiment 1.
The growth result of spinning roller worm culture solution promotion Mixed Microbes: from the figure 3, it may be seen that being added to the training of spinning roller worm in inoculum
The addition group of nutrient solution, compared with the control group for being not added with spinning roller worm culture solution, Mixed Microbes dry cell weight increases 52.9%, shows
Spinning roller worm culture solution has apparent facilitation effect to the growth of Mixed Microbes.
Claims (1)
1. a kind of method for promoting flocculability bacterial growth using spinning roller worm culture solution, it is characterised in that the specific steps of this method
Are as follows:
A. spinning roller worm is placed in spinning roller worm culture solution and is bred, when the density of spinning roller worm reaches 100~500ind/mL,
Spinning roller worm is removed with 300 mesh nylon net filters；
B. obtained culture solution ultrafiltration membrance filter after filtering in step a further removes the tiny particles content in filtrate；
C. the flocculability bacterium that inclined-plane saves is seeded in 100mL LB culture medium with oese, is subsequently placed in shaking for 120rpm
On bed, in 30 DEG C continuous culture 24 hours, centrifugal treating culture solution is washed with deionized, with sterile water by bacterial concentration tune
It saves to OD600Value is 0.6~0.8, this solution is bacterium bacteria suspension；
D. bacterium bacteria suspension that step c is obtained is added to 5%~10% additive amount of simulated domestic wastewater volume sterilized
In the simulated domestic wastewater of bacterium to get arrive bacteria culture media；
E. spinning roller worm culture solution obtained by step b is added to step d with 0.05%~1% additive amount of bacteria culture media volume
It in resulting bacteria culture media, is subsequently placed on the shaking table of 120rpm, in 30 DEG C of continuous cultures 48 hours to get to containing bacterium
The culture solution of floccule body；
The flocculability bacterium are as follows: vesicle shortwave monad, bacillus cereus, any one in bacillus thuringiensis
Or it is any several；
The simulated domestic wastewater formula are as follows: 0.17g glucose, 0.16g starch, 0.233g sodium acetate, 0.025g ammonium chloride,
0.158g peptone, 0.04g beef extract, 0.0284g ammonium sulfate, 0.07g potassium dihydrogen phosphate and 0.06g sodium carbonate, and
1000mL deionized water.
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| CN110894482A (en) * | 2019-12-17 | 2020-03-20 | 上海大学 | Method for promoting growth of flocculating bacteria |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07155791A (en) * | 1993-12-09 | 1995-06-20 | Agency Of Ind Science & Technol | Method for purifying organic polluted waste water |
| JPH08267094A (en) * | 1995-03-28 | 1996-10-15 | Lion Corp | Waste water treatment in production of alpha-sulfofatty acid alkyl ester |
| CN101455192A (en) * | 2009-01-04 | 2009-06-17 | 上海大学 | Bdelloid rotifers mass culture method |
| CN101704612A (en) * | 2009-11-19 | 2010-05-12 | 长安大学 | Integrated treatment process of enhanced flocculation and bio-contact oxidation for high-salt oil-containing wastewater |
-
2016
- 2016-03-03 CN CN201610119990.XA patent/CN105647831B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07155791A (en) * | 1993-12-09 | 1995-06-20 | Agency Of Ind Science & Technol | Method for purifying organic polluted waste water |
| JPH08267094A (en) * | 1995-03-28 | 1996-10-15 | Lion Corp | Waste water treatment in production of alpha-sulfofatty acid alkyl ester |
| CN101455192A (en) * | 2009-01-04 | 2009-06-17 | 上海大学 | Bdelloid rotifers mass culture method |
| CN101704612A (en) * | 2009-11-19 | 2010-05-12 | 长安大学 | Integrated treatment process of enhanced flocculation and bio-contact oxidation for high-salt oil-containing wastewater |
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