CN105647795A - Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique - Google Patents
Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique Download PDFInfo
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- CN105647795A CN105647795A CN201610104055.6A CN201610104055A CN105647795A CN 105647795 A CN105647795 A CN 105647795A CN 201610104055 A CN201610104055 A CN 201610104055A CN 105647795 A CN105647795 A CN 105647795A
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- microbial enzyme
- pcr
- pcr instrument
- instrument
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/025—Displaying results or values with integrated means
- B01L2300/027—Digital display, e.g. LCD, LED
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
Abstract
The invention relates to a novel microbial enzyme rapid detection device based on a PCR (polymerase chain reaction) technique. The device comprises a PCR instrument, a display screen, an initial parameter setting panel, a reactant inlet, an upper equipment end cap, a cooling window, a microbial enzyme detection instrument and a data analysis computer, wherein the display screen is arranged on the upper surface of the PCR instrument, the initial parameter setting panel is arranged on the side surface of the display screen, the upper equipment end cap is arranged at the top of the PCR instrument, the reactant inlet is formed in the upper surface of the upper equipment end cap, the cooling window is formed in the side surface of the PCR instrument, a cooling fan is mounted in the cooling window, and the rear side of the PCR instrument is connected with the microbial enzyme detection instrument. The device has the benefits as follows: pollution caused by oscillation among microorganisms in the transfer process is greatly reduced; an intelligent temperature control system is adopted, a heating part and a refrigeration part operate separately, the reaction temperature can be quickly changed, and the reaction effect of a PCR is guaranteed.
Description
Technical field
The present invention relates to microbe rapid detection device field, be specifically related to the novel microbial enzyme device for fast detecting of PCR-based technology.
Background technology
PCR (polymerase chain reaction) is that a kind of for amplifying the Protocols in Molecular Biology expanding specific DNA fragmentation, it is considered as the special DNA replication dna of in vitro, and the maximum feature of PCR, is to be significantly increased by the DNA of trace. Polymerase chain reaction is to utilize DNA 95 �� of high temperature time variations Celsius in vitro can become strand, during low temperature, primer is combined by the principle of base pair complementarity with strand, temperature regulating is to archaeal dna polymerase optimal reactive temperature again, and archaeal dna polymerase is along phosphoric acid to the direction composition complementary strand of pentose. Microbial rapid detection instrument is a kind of portable microorganism Quantitative detection instrument. Convenient and swift, namely three steps can detect that result, and detection, without professional training, has high specific, and detection bottle can use whenever and wherever possible, and specificity is up to 99.999%; Portable microorganism Quantitative detection instrument is exactly laboratory, safely discards after actually used; Detection rapidly, is 2-5 times of conventional inspection method; There is high sensitivity, 1 objective microbe can be accurately detected, but existing round pcr exists following drawback: the cross-contamination of target sequence or amplified production; Template always contains impurity and causes occurring without amplified band; The concentration of primer and Mg2+ concentration are up to causing that pcr amplification failure does not go out amplified band, and temperature controls instability etc.
Summary of the invention
In order to solve above-mentioned prior art Problems existing, the present invention provides the novel microbial enzyme device for fast detecting of PCR-based technology.
The present invention is achieved through the following technical solutions above-mentioned purpose:
The novel microbial enzyme device for fast detecting of PCR-based technology, including PCR instrument, display screen, initial parameter arranges panel, reactant entrance, equipment upper end cover, thermal window, microbial enzyme detector, Data Analysis Computer, described PCR instrument upper surface is provided with described display screen, described display screen side is provided with described initial parameter and arranges panel, described PCR instrument top is described equipment upper end cover, described equipment upper end cover upper surface has described reactant entrance, described PCR instrument side is provided with described thermal window, described thermal window is internally installed radiator fan, described PCR instrument rear is connected to described microbial enzyme detector, described microbial enzyme detector outfan is provided with holding wire, the described holding wire end connects described Data Analysis Computer, the porch of described PCR instrument is provided with reactant classification ware, described reactant classification ware rear is provided with autoclave sterilization unit, described autoclave sterilization unit rear is set side by side with template analysis instrument and Mg2+ ion detection pointer, described template analysis instrument and described Mg2+ ion detection pointer rear are provided with primer concentration equilibristat, described primer concentration equilibristat passes into alternating temperature water-bath pond, around described alternating temperature water-bath pond, alternating temperature aluminium block is installed, described alternating temperature aluminium block is connected to heater and compression refigerating machine, described heater and described compression refigerating machine are connected to Intelligent temperature controller, described central controller is arranged on described PCR instrument center of inside and connects miscellaneous part, described microbial enzyme detector is connected by shock insulation conducting tube with described PCR instrument, the some detection samples of described microbial enzyme detector surface configuration contain ware, described microbial enzyme detector outfan is provided with data cable connector.
Wherein, described display screen is embedded in described PCR instrument upper surface and connects the circuit board of inside, described initial parameter arranges panel and is arranged on the electronics base of described PCR instrument upper surface with the form of button, described equipment upper end cover is hinged with described PCR instrument main body, and described reactant entrance is passed into inside described PCR instrument by pipeline.
Wherein, described thermal window is arranged in the framework of described PCR instrument side, the center of described radiator fan is arranged point-blank with described thermal window, described microbial enzyme detector and described PCR instrument are tightly connected by described shock insulation conducting tube, and described microbial enzyme detector is connected with described Data Analysis Computer by described holding wire.
Wherein, described reactant classification ware seals with described reactant entrance and communicates, described autoclave sterilization unit is connected by pipeline with described reactant classification ware, described template analysis instrument and described Mg2+ ion detection pointer seal with described autoclave sterilization unit and communicate, and outfan is all connected to described central controller.
Wherein, described primer concentration equilibristat is connected by data wire with described central controller, outfan passes into described alternating temperature water-bath pond by pipeline, described alternating temperature aluminium block and described alternating temperature water-bath pond are adjacent to installation, described heater and described compression refigerating machine and all connect described alternating temperature aluminium block by lines of thermal conduction.
Wherein, described Intelligent temperature controller connects described heater and described compression refigerating machine by controlling cable, described central controller is arranged on described PCR instrument center of inside by screw and connects miscellaneous part by cable, described detection sample contains ware transparent material and designs, inside straight-through described microbial enzyme detector, described data cable connector is connected to the control module within described microbial enzyme detector by data wire.
The beneficial effects of the present invention is: PCR instrument and detector integrated design, accelerate detection efficiency, considerably reduce the pollution between the microorganism that the concussion in transfer process causes simultaneously; Intelligentized temperature control system, heating and refrigerating part are separately operable, it is possible to quickly change reaction temperature, it is ensured that the reaction effect of polymerase chain reaction; Template analysis instrument fully analyzes the composition of template, it is ensured that with the goodness of fit of required composition; Mg2+ ion detection pointer can detect Mg2+ ion concentration in real time, it is ensured that the appearance of amplified band.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the axonometric chart of the novel microbial enzyme device for fast detecting of PCR-based technology of the present invention;
Fig. 2 is the interior views of the PCR instrument of the novel microbial enzyme device for fast detecting of PCR-based technology of the present invention;
Fig. 3 is the front view of the microbial enzyme detector of the novel microbial enzyme device for fast detecting of PCR-based technology of the present invention.
Detailed description of the invention
As shown in Figure 1-Figure 3, the novel microbial enzyme device for fast detecting of PCR-based technology, including PCR instrument 1, display screen 2, initial parameter arranges panel 3, reactant entrance 4, equipment upper end cover 5, thermal window 6, microbial enzyme detector 7, Data Analysis Computer 9, described PCR instrument 1 upper surface is provided with described display screen 2, described display screen 2 side is provided with described initial parameter and arranges panel 3, described PCR instrument 1 top is described equipment upper end cover 5, described equipment upper end cover 5 upper surface has described reactant entrance 4, described PCR instrument 1 side is provided with described thermal window 6, described thermal window 6 is internally installed radiator fan 21, described PCR instrument 1 rear is connected to described microbial enzyme detector 7, described microbial enzyme detector 7 outfan is provided with holding wire 8, described holding wire 8 end connects described Data Analysis Computer 9, the porch of described PCR instrument 1 is provided with reactant classification ware 10, described reactant classification ware 10 rear is provided with autoclave sterilization unit 11, described autoclave sterilization unit 11 rear is set side by side with template analysis instrument 12 and Mg2+ ion detection pointer 13, described template analysis instrument 12 and described Mg2+ ion detection pointer 13 rear are provided with primer concentration equilibristat 14, described primer concentration equilibristat 14 passes into alternating temperature water-bath pond 15, around described alternating temperature water-bath pond 15, alternating temperature aluminium block 16 is installed, described alternating temperature aluminium block 16 is connected to heater 17 and compression refigerating machine 18, described heater 17 and described compression refigerating machine 18 are connected to Intelligent temperature controller 19, described central controller 20 is arranged on described PCR instrument 1 center of inside and connects miscellaneous part, described microbial enzyme detector 7 is connected by shock insulation conducting tube 23 with described PCR instrument 1, the some detection samples of described microbial enzyme detector 7 surface configuration contain ware 22, described microbial enzyme detector 7 outfan is provided with data cable connector 24.
In the present embodiment, described display screen 2 is embedded in described PCR instrument 1 upper surface and connects the circuit board of inside, described initial parameter arranges panel 3 and is arranged on the electronics base of described PCR instrument 1 upper surface with the form of button, described equipment upper end cover 5 is hinged with described PCR instrument 1 main body, and it is internal that described reactant entrance 4 is passed into described PCR instrument 1 by pipeline.
In the present embodiment, described thermal window 6 is arranged in the framework of described PCR instrument 1 side, the center of described radiator fan 21 and described thermal window 6 arrange point-blank, described microbial enzyme detector 7 is tightly connected by described shock insulation conducting tube 23 with described PCR instrument 1, and described microbial enzyme detector 7 is connected with described Data Analysis Computer 9 by described holding wire 8.
In the present embodiment, described reactant classification ware 10 seals with described reactant entrance 4 and communicates, described autoclave sterilization unit 11 is connected by pipeline with described reactant classification ware 10, described template analysis instrument 12 and described Mg2+ ion detection pointer 13 seal with described autoclave sterilization unit 11 and communicate, and outfan is all connected to described central controller 20.
In the present embodiment, described primer concentration equilibristat 14 is connected by data wire with described central controller 20, outfan passes into described alternating temperature water-bath pond 15 by pipeline, described alternating temperature aluminium block 16 is adjacent to installation with described alternating temperature water-bath pond 15, and described heater 17 and described compression refigerating machine 18 all connect described alternating temperature aluminium block 16 by lines of thermal conduction.
In the present embodiment, described Intelligent temperature controller 19 connects described heater 17 and described compression refigerating machine 18 by controlling cable, described central controller 20 is arranged on described PCR instrument 1 center of inside by screw and connects miscellaneous part by cable, described detection sample contains ware 22 and designs with transparent material, straight-through described microbial enzyme detector 7 is internal, and described data cable connector 24 is connected to the control module within described microbial enzyme detector 7 by data wire.
User is when using the present invention, panel is set in initial parameter parameter is set, reacted for microbial enzyme thing entrance is put into, raw material enters PCR instrument and reacts, first carry out disinfection at autoclave sterilization unit, analyze the composition of template then through template analysis instrument, and detect the concentration of Mg2+ through Mg2+ ion detection pointer, carrying out entrance alternating temperature water-bath pond after ultimate density is determined then through primer concentration equilibristat, composition result demonstrates at display screen.
Real time temperature is reached central controller by Intelligent temperature controller, and control radiator fan and heater and compression refigerating machine carry out the control of temperature in real time.
User is when using the present invention, and microbial enzyme detector receives the complete detection thing of reaction, respectively enters multiple reactant classification ware and tests, through holding wire by incoming for result Data Analysis Computer after.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described. Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention. Claimed scope is defined by appending claims and equivalent thereof.
Claims (6)
1. the novel microbial enzyme device for fast detecting of PCR-based technology, it is characterized in that: include PCR instrument, display screen, initial parameter arranges panel, reactant entrance, equipment upper end cover, thermal window, microbial enzyme detector, Data Analysis Computer, described PCR instrument upper surface is provided with described display screen, described display screen side is provided with described initial parameter and arranges panel, described PCR instrument top is described equipment upper end cover, described equipment upper end cover upper surface has described reactant entrance, described PCR instrument side is provided with described thermal window, described thermal window is internally installed radiator fan, described PCR instrument rear is connected to described microbial enzyme detector, described microbial enzyme detector outfan is provided with holding wire, the described holding wire end connects described Data Analysis Computer, the porch of described PCR instrument is provided with reactant classification ware, described reactant classification ware rear is provided with autoclave sterilization unit, described autoclave sterilization unit rear is set side by side with template analysis instrument and Mg2+ ion detection pointer, described template analysis instrument and described Mg2+ ion detection pointer rear are provided with primer concentration equilibristat, described primer concentration equilibristat passes into alternating temperature water-bath pond, around described alternating temperature water-bath pond, alternating temperature aluminium block is installed, described alternating temperature aluminium block is connected to heater and compression refigerating machine, described heater and described compression refigerating machine are connected to Intelligent temperature controller, described central controller is arranged on described PCR instrument center of inside and connects miscellaneous part, described microbial enzyme detector is connected by shock insulation conducting tube with described PCR instrument, the some detection samples of described microbial enzyme detector surface configuration contain ware, described microbial enzyme detector outfan is provided with data cable connector.
2. the novel microbial enzyme device for fast detecting of PCR-based technology according to claim 1, it is characterized in that: described display screen is embedded in described PCR instrument upper surface and connects the circuit board of inside, described initial parameter arranges panel and is arranged on the electronics base of described PCR instrument upper surface with the form of button, described equipment upper end cover is hinged with described PCR instrument main body, and described reactant entrance is passed into inside described PCR instrument by pipeline.
3. the novel microbial enzyme device for fast detecting of PCR-based technology according to claim 1, it is characterized in that: described thermal window is arranged in the framework of described PCR instrument side, the center of described radiator fan is arranged point-blank with described thermal window, described microbial enzyme detector and described PCR instrument are tightly connected by described shock insulation conducting tube, and described microbial enzyme detector is connected with described Data Analysis Computer by described holding wire.
4. the novel microbial enzyme device for fast detecting of PCR-based technology according to claim 1, it is characterized in that: described reactant classification ware seals with described reactant entrance and communicates, described autoclave sterilization unit is connected by pipeline with described reactant classification ware, described template analysis instrument and described Mg2+ ion detection pointer seal with described autoclave sterilization unit and communicate, and outfan is all connected to described central controller.
5. the novel microbial enzyme device for fast detecting of PCR-based technology according to claim 1, it is characterized in that: described primer concentration equilibristat is connected by data wire with described central controller, outfan passes into described alternating temperature water-bath pond by pipeline, described alternating temperature aluminium block and described alternating temperature water-bath pond are adjacent to installation, described heater and described compression refigerating machine and all connect described alternating temperature aluminium block by lines of thermal conduction.
6. the novel microbial enzyme device for fast detecting of PCR-based technology according to claim 1, it is characterized in that: described Intelligent temperature controller connects described heater and described compression refigerating machine by controlling cable, described central controller is arranged on described PCR instrument center of inside by screw and connects miscellaneous part by cable, described detection sample contains ware transparent material and designs, inside straight-through described microbial enzyme detector, described data cable connector is connected to the control module within described microbial enzyme detector by data wire.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610104055.6A CN105647795A (en) | 2016-02-25 | 2016-02-25 | Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique |
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| CN201610104055.6A CN105647795A (en) | 2016-02-25 | 2016-02-25 | Novel microbial enzyme rapid detection device based on PCR (polymerase chain reaction) technique |
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Citations (6)
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| CN2612673Y (en) * | 2003-03-27 | 2004-04-21 | 上海复生生物工程研究所有限公司 | Full-automatic water-bath type gene amplification |
| US20050032072A1 (en) * | 2003-08-08 | 2005-02-10 | Perlegen Sciences, Inc. | Fragmentation and labelling with a programmable temperature control module |
| CN101824486A (en) * | 2009-12-30 | 2010-09-08 | 华东医学生物技术研究所 | Method and device for rapidly detecting nucleic acid |
| CN103642683A (en) * | 2013-12-19 | 2014-03-19 | 江苏金太生命科技有限公司 | Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments |
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2016
- 2016-02-25 CN CN201610104055.6A patent/CN105647795A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2226161A (en) * | 1988-11-10 | 1990-06-20 | Grant Instr | Programmable waterbath apparatus for control of rapid temperature cycling |
| CN2479506Y (en) * | 2001-04-13 | 2002-02-27 | 阎小君 | Quantitative PCR gene magnification diagnostic instrument |
| CN2612673Y (en) * | 2003-03-27 | 2004-04-21 | 上海复生生物工程研究所有限公司 | Full-automatic water-bath type gene amplification |
| US20050032072A1 (en) * | 2003-08-08 | 2005-02-10 | Perlegen Sciences, Inc. | Fragmentation and labelling with a programmable temperature control module |
| CN101824486A (en) * | 2009-12-30 | 2010-09-08 | 华东医学生物技术研究所 | Method and device for rapidly detecting nucleic acid |
| CN103642683A (en) * | 2013-12-19 | 2014-03-19 | 江苏金太生命科技有限公司 | Polymerase chain reaction (PCR) instrument capable of performing multiple PCR experiments |
Non-Patent Citations (1)
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