CN105633272A - Piezoelectric fiber-based myocardial cell generator and preparation method thereof - Google Patents
Piezoelectric fiber-based myocardial cell generator and preparation method thereof Download PDFInfo
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- 230000002107 myocardial effect Effects 0.000 title claims abstract description 106
- 239000000835 fiber Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims description 12
- 238000000034 method Methods 0.000 claims abstract description 39
- 239000004205 dimethyl polysiloxane Substances 0.000 claims abstract description 38
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 38
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims abstract description 38
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 38
- 239000000758 substrate Substances 0.000 claims abstract description 31
- 239000002184 metal Substances 0.000 claims abstract description 26
- 229910052751 metal Inorganic materials 0.000 claims abstract description 26
- 239000011521 glass Substances 0.000 claims abstract description 9
- 239000004744 fabric Substances 0.000 claims description 127
- 210000004027 cell Anatomy 0.000 claims description 109
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 12
- 239000005357 flat glass Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 10
- OGBQILNBLMPPDP-UHFFFAOYSA-N 2,3,4,7,8-Pentachlorodibenzofuran Chemical compound O1C2=C(Cl)C(Cl)=C(Cl)C=C2C2=C1C=C(Cl)C(Cl)=C2 OGBQILNBLMPPDP-UHFFFAOYSA-N 0.000 claims description 6
- 238000001523 electrospinning Methods 0.000 claims description 6
- 239000011787 zinc oxide Substances 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 5
- 238000012986 modification Methods 0.000 claims description 5
- 239000000919 ceramic Substances 0.000 claims description 3
- 230000005684 electric field Effects 0.000 claims description 3
- 210000000107 myocyte Anatomy 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 229920003213 poly(N-isopropyl acrylamide) Polymers 0.000 abstract 2
- 239000010408 film Substances 0.000 description 23
- 239000000463 material Substances 0.000 description 9
- 238000010248 power generation Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000010041 electrostatic spinning Methods 0.000 description 2
- 230000010287 polarization Effects 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 206010001497 Agitation Diseases 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000010010 raising Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
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- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N30/00—Piezoelectric or electrostrictive devices
- H10N30/1061—Piezoelectric or electrostrictive devices based on piezoelectric or electrostrictive fibres
-
- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02N—ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR
- H02N2/00—Electric machines in general using piezoelectric effect, electrostriction or magnetostriction
- H02N2/18—Electric machines in general using piezoelectric effect, electrostriction or magnetostriction producing electrical output from mechanical input, e.g. generators
-
- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02N—ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR
- H02N2/00—Electric machines in general using piezoelectric effect, electrostriction or magnetostriction
- H02N2/22—Methods relating to manufacturing, e.g. assembling, calibration
-
- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N30/00—Piezoelectric or electrostrictive devices
- H10N30/01—Manufacture or treatment
-
- H—ELECTRICITY
- H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
- H10N30/00—Piezoelectric or electrostrictive devices
- H10N30/30—Piezoelectric or electrostrictive devices with mechanical input and electrical output, e.g. functioning as generators or sensors
Abstract
The invention provides a method for preparing a piezoelectric fiber-based myocardial cell generator. The method comprises the following steps: preparing a glass plate, and coating the glass plate with poly (N-isopropylacrylamide) (PNIPAM) to form a sacrificial layer; coating the sacrificial layer with PDMS to form a semi-solidified PDMS film substrate; semi-embedding two metal electrodes into two ends of the semi-solidified PDMS film substrate respectively; forming a piezoelectric fiber layer on the semi-solidified PDMS film substrate which is semi-embedded with the metal electrodes, making the arrangement directions of a plurality of piezoelectric fibers of forming the piezoelectric fiber layer consistent and connecting the two metal electrodes; uploading myocardial cells to the piezoelectric fiber layer, and arranging and growing the myocardial cells along the length directions of the piezoelectric fibers in the piezoelectric fiber layer; connecting the two metal electrodes respectively to obtain the myocardial cell generator; removing the sacrificial layer and stripping the glass plate from the myocardial cell generator. The method effectively solves the problem of oriented alignment of the myocardial cells and achieves maximization of the energy conversion efficiency.
Description
Technical field
The invention belongs to material field, specifically, the present invention relates to the myocardial cell's generator based on piezoelectric fabric and its preparation method.
Background technology
The development in pluralism of modern science and technology, that energy requirement span is drawn is very wide, the big power consumption demand of existing terawatt level, has again the low power consumption electronics being low to moderate a few microwatt. Wherein low power consumption electronics major face is applied to wearable electronics and implantable medicine equipment. Owing to application demand is more individualized and portability, so self-powered is low power consumption electronic energy problem developing direction. Collection of energy is considered as one of preferred option realizing self-powered system. Collection of energy (Energyharvesting) is that environmental energy is converted to electric energy, and the substitute as battery is that electronics is powered.
Summary of the invention
One of technical problem that the present invention is intended to solve in correlation technique at least to a certain extent. For this reason, it is an object of the present invention to propose a kind of method preparing the myocardial cell's generator based on piezoelectric fabric and the myocardial cell's generator based on piezoelectric fabric utilizing the method to prepare. The myocardial cell's generator based on piezoelectric fabric utilizing the method to prepare can solve the oriented alignment problem of myocardial cell and the compatibility issue of myocardial cell and piezoelectric effectively, and then can effectively realize maximumization of effciency of energy transfer.
According to an aspect of the present invention, the present invention proposes a kind of method preparing the myocardial cell's generator based on piezoelectric fabric, comprising:
Prepare one piece of sheet glass, and apply PNIPAM on the glass plates, to form sacrifice layer;
PDMS is applied, to form the PDMS film substrate of semicure on described sacrifice layer;
Half two metal electrodes are embedded respectively to the two ends on the PDMS film substrate of described semicure;
Forming piezoelectric fabric layer on the PDMS film substrate of semicure being partly embedded with metal electrode, the many piezoelectric fabric arragement directions forming described piezoelectric fabric layer unanimously and connect two described metal electrodes;
Uploading myocardial cell on described piezoelectric fabric layer, described myocardial cell arranges and growth along the length direction of the piezoelectric fabric in described piezoelectric fabric layer;
Described two metal electrodes are connected electric capacity respectively, to obtain described myocardial cell's generator; And
Remove described sacrifice layer, and described myocardial cell's generator is peeled off described sheet glass.
The preparation of the above embodiment of the present invention is based on the method for myocardial cell's generator of piezoelectric fabric, utilize the oriented alignment of piezoelectric fabric control and realize oriented alignment and the growth of myocardial cell ingeniously, and then achieve the compatibility of cell and transductive material, obtain the 3-D solid structure of cell and piezoelectric fabric, thus obtain high performance myocardial cell's generator.
In addition, preparation according to the above embodiment of the present invention can also have following additional technology feature based on the method for myocardial cell's generator of piezoelectric fabric:
In some embodiments of the invention, by adopting, high pressure far field electrospinning process prepares described piezoelectric fabric layer.
In some embodiments of the invention, described piezoelectric fabric is prepared by any one in employing PZT, PCDF and zinc oxide.
In some embodiments of the invention, described high pressure far field electrospinning process utilizes the external electric field that ground-electrode and impressed voltage are set up so that piezoelectric fabric falls along specific direction and arrangement.
In some embodiments of the invention, before described piezoelectric fabric layer uploads myocardial cell, comprise further:
Use adhesion protein that described piezoelectric fabric layer is carried out surface modification, so that described Myocyte growth and sticking on described piezoelectric fabric layer.
According to the second aspect of the invention, the invention allows for a kind of myocardial cell's generator based on piezoelectric fabric utilizing foregoing method to prepare, described myocardial cell's generator based on piezoelectric fabric comprises:
PDMS film substrate;
Two electrodes, described two electrodes partly embed the upper surface of described PDMS film substrate respectively;
Piezoelectric fabric layer, described piezoelectric fabric layer load is at the upper surface of described PDMS film substrate, and described piezoelectric fabric layer is made up of the piezoelectric fabric that many arragement directions are consistent, and described piezoelectric fabric connects described two electrodes;
Myocardial cell's layer, described myocardial cell's layer load is on described piezoelectric fabric layer, and described myocardial cell's layer is made up of multiple myocardial cell, and myocardial cell described in each is along the length direction arrangement of described piezoelectric fabric and growth; And
Electric capacity, described electric capacity is connected with described two electrodes respectively.
In some embodiments of the invention, described piezoelectric fabric is any one in PZT piezoelectric ceramic fibers, PCDF piezoelectric fabric and zinc oxide piezoelectric fiber.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet preparing the myocardial cell's generator based on piezoelectric fabric according to an embodiment of the invention.
Fig. 2 is the schematic diagram utilizing according to an embodiment of the invention and preparing piezoelectric fabric layer prepared by the method based on myocardial cell's generator of piezoelectric fabric.
Fig. 3 utilizes the schematic diagram preparing myocardial cell's layer prepared by the method based on myocardial cell's generator of piezoelectric fabric according to an embodiment of the invention.
Fig. 4 is the structural representation of myocardial cell's generator based on piezoelectric fabric according to an embodiment of the invention.
Embodiment
Being described below in detail embodiments of the invention, the example of embodiment is shown in the drawings, and wherein same or similar label represents same or similar element or has element that is identical or similar functions from start to finish. It is exemplary below by the embodiment being described with reference to the drawings, it is intended to for explaining the present invention, and limitation of the present invention can not be interpreted as.
Myocardial cell's generator is the energy transformation model that the mechanical energy of myocardial cell's self-constriction is converted to electric energy. The compatible mechanism of myocardial cell and transductive material is inquired into, is conducive to disease model, the research of the aspects such as organizational project. Cell generator model can customize micro-power generator in coenocorrelation, it is also possible to is implanted in body, powers to electron device in body. But, still there are some technical problems in existing myocardial cell's generator at present. Such as, the people such as T.Ishisaka utilize single root PZT piezoelectric fabric that the mechanical energy of the myocardial cell grown on PDMS film is converted to electric energy, but the randomness of myocardial cell makes voltage export only 10mV; The people such as E.Choi devise a kind of concentric apertures structure, it is possible to make cell along the arrangement of Communication ray coil structures, but the discordance that cell machinery shrinks makes electrical signal output irregular.
For this reason, according to an aspect of the present invention, the present invention proposes a kind of method preparing the myocardial cell's generator based on piezoelectric fabric, preparation according to the specific embodiment of the invention comprises based on the method for myocardial cell's generator of piezoelectric fabric: prepare one piece of sheet glass, and apply PNIPAM on a glass, to form sacrifice layer; PDMS is applied, to form the PDMS film substrate of semicure on sacrifice layer; Half two metal electrodes are embedded respectively to the two ends on the PDMS film substrate of semicure; Forming piezoelectric fabric layer on the PDMS film substrate of semicure being partly embedded with metal electrode, many piezoelectric fabric arragement directions of composition piezoelectric fabric layer are consistent and connect two metal electrodes; Uploading myocardial cell on piezoelectric fabric layer, myocardial cell arranges and growth along the length direction of the piezoelectric fabric in piezoelectric fabric layer; Two metal electrodes are connected electric capacity respectively, to obtain myocardial cell's generator; And removing sacrifice layer, and myocardial cell's generator is peeled off sheet glass.
The preparation of the above embodiment of the present invention is based on the method for myocardial cell's generator of piezoelectric fabric, utilize the oriented alignment of piezoelectric fabric control and realize oriented alignment and the growth of myocardial cell ingeniously, and then achieve the compatibility of cell and transductive material, obtain the 3-D solid structure of cell and piezoelectric fabric, thus obtain high performance myocardial cell's generator.
According to a particular embodiment of the invention, the method for the myocardial cell's generator being prepared in piezoelectric fabric of the specific embodiment of the invention is described in detail below with reference to Fig. 1.
According to a particular embodiment of the invention, first, prepare one piece of sheet glass, and apply PNIPAM on a glass, to form sacrifice layer. Sheet glass wherein is only as the effect of service platform or anchoring base, and the sacrifice layer formed on a glass may be used for fixing follow-up PDMS film substrate. This sacrifice layer has cohesive action at 37 DEG C, can dissolve when 22 DEG C. Therefore, adopting and utilize this sacrifice layer can play fixed action well, stripping liquid is more convenient simultaneously, and then the PDMS film substrate formed in the above can not be caused damage.
According to a particular embodiment of the invention, further, on sacrifice layer obtained above, PDMS is applied, to form the PDMS film substrate of semicure; And half embed two metal electrodes respectively to the two ends on the PDMS film substrate of semicure. Thus, the PDMS film substrate of semicure has certain viscosity, and then can be fixed by metal electrode, in addition, it is also possible to be fixed by the follow-up piezoelectric fabric layer formed on its surface.
According to a particular embodiment of the invention, further, forming piezoelectric fabric layer on the PDMS film substrate of semicure being partly embedded with metal electrode, many piezoelectric fabric arragement directions of composition piezoelectric fabric layer are consistent and connect two metal electrodes. Thus, the arragement direction of piezoelectric fabric is consistent, it is possible to improve the efficiency of conversion of energy further.
According to a particular embodiment of the invention, by adopting, high pressure far field electrospinning process prepares piezoelectric fabric layer. And by the specific fiber collecting electrode of design. According to a particular embodiment of the invention, high pressure far field electrospinning process utilizes the external electric field that ground-electrode and impressed voltage are set up so that piezoelectric fabric falls along specific direction and arrangement, as shown in Figure 2. The piezoelectric fabric arragement direction thus making to be formed piezoelectric fabric layer is consistent, and then can improve the efficiency of conversion of energy further.
According to a particular embodiment of the invention, piezoelectric fabric is prepared by any one in employing PZT, PCDF and zinc oxide. Thus, the fiber prepared all has good piezoelectricity. And by the high voltage polarization in electrostatic spinning process and mechanical stretching process so that piezoelectric property is better than thin-film material.
According to a particular embodiment of the invention, further, uploading myocardial cell on piezoelectric fabric layer, myocardial cell arranges and growth along the length direction of the piezoelectric fabric in piezoelectric fabric layer, as shown in Figure 3. According to a particular embodiment of the invention, the mechanical characteristics of myocardial cell comprises shrinkability, autorhymicity, excitability and refractoriness, and it shrinks based on flesh silk slip principle. The present inventor make use of the physical guide effect of piezoelectricity fibre sucking ingeniously, myocardial cell along the length direction arrangement of piezoelectric fabric and is grown, and then make the shrinkage direction of myocardial cell consistent with the arragement direction of piezoelectric fabric, it is achieved that maximumization of energy conversion efficiency.
According to a particular embodiment of the invention, before piezoelectric fabric layer uploads myocardial cell, comprise further: use adhesion protein that piezoelectric fabric layer is carried out surface modification, so that Myocyte growth and sticking on piezoelectric fabric layer. Myocardial cell thus can be made to grow better on described piezoelectric fabric, and the bonding force of both raisings can be done, and then make to shrink during myocardial cell to drive piezoelectric fabric to greatest extent, and then improve the turnover ratio of efficiency. In addition, by aforesaid method, piezoelectric fabric layer is carried out surface modification, it is also possible to effectively solve the compatibility issue of myocardial cell and piezoelectric fabric, and then improve the surviving rate of myocardial cell.
According to a particular embodiment of the invention, further, two metal electrodes are connected electric capacity respectively, to obtain myocardial cell's generator; And removing sacrifice layer, and myocardial cell's generator is peeled off sheet glass. According to a particular embodiment of the invention, two metal electrodes being connected electric capacity respectively, mycardial contractility can be driven piezoelectric fabric, the electric charge of generation is derived by electrode, and then generating. Owing to sacrifice layer only plays fixing effect, finally only needing to be placed in normal temperature environment and be cooled to 22 degrees Celsius, namely solubilized removes.
According to myocardial cell's generator that the above embodiment of the present invention method prepares, efficiently solve the oriented alignment of myocardial cell and the compatibility of growth and cell and piezoelectric fabric. The kinetic energy of myocardial cell can be converted into electric energy by this myocardial cell's generator effectively substantially, and power generation stabilization height, peak voltage exports can reach 200mV. Therefore, the myocardial cell's generator utilizing the above embodiment of the present invention method to prepare has good power generation performance.
According to another aspect of the present invention, the invention allows for the myocardial cell's generator based on piezoelectric fabric utilizing foregoing method to prepare.
According to a particular embodiment of the invention, describe the myocardial cell's generator based on piezoelectric fabric of the specific embodiment of the invention in detail below with reference to Fig. 4, it comprises: PDMS film substrate 10, two electrodes 20, piezoelectric fabric layer 30, myocardial cell's layer 40 and electric capacity 50.
Wherein, PDMS film substrate 10, as supporting role, for carrying piezoelectric fabric, enables piezoelectric fabric arrange according to a certain direction. Two electrodes 20 half embed the upper surface of PDMS film substrate 10 respectively; For deriving the electric charge that piezoelectric fabric produces; Piezoelectric fabric layer 30 load is at the upper surface of PDMS film substrate, and piezoelectric fabric layer 30 is made up of the piezoelectric fabric that many arragement directions are consistent, and described piezoelectric fabric connects two electrodes; Myocardial cell's layer 40 load is on described piezoelectric fabric layer 30, and myocardial cell's layer 40 is made up of multiple myocardial cell, and each myocardial cell is along the length direction arrangement of piezoelectric fabric and growth; And electric capacity 50 is connected with two electrodes 20 respectively.
Myocardial cell's generator of the piezoelectric fabric of the above embodiment of the present invention, make use of oriented alignment and growth that the oriented alignment of piezoelectric fabric controls and realizes myocardial cell ingeniously, and then achieve the compatibility of cell and transductive material, obtain the 3-D solid structure of cell and piezoelectric fabric, thus obtain high performance myocardial cell's generator.
According to a particular embodiment of the invention, piezoelectric fabric is any one in PZT piezoelectric ceramic fibers, PCDF piezoelectric fabric and zinc oxide piezoelectric fiber. Thus, the fiber prepared all has good piezoelectricity. And by the high voltage polarization in electrostatic spinning process and mechanical stretching process so that piezoelectric property is better than thin-film material.
The kinetic energy of myocardial cell can be converted into electric energy by this myocardial cell's generator effectively substantially, and power generation stabilization height, peak voltage exports can reach 200mV. Therefore, the myocardial cell's generator utilizing the above embodiment of the present invention method to prepare has good power generation performance.
Embodiment 1
Preparation myocardial cell's generator.
Prepare according to the step shown in Fig. 1, comprising: prepare one piece of sheet glass, and apply PNIPAM on a glass, to form sacrifice layer; PDMS is applied, to form the PDMS film substrate of semicure on sacrifice layer; Half two metal electrodes are embedded respectively to the two ends on the PDMS film substrate of semicure; Forming piezoelectric fabric layer on the PDMS film substrate of semicure being partly embedded with metal electrode, many piezoelectric fabric arragement directions of composition piezoelectric fabric layer are consistent and connect two metal electrodes; Uploading myocardial cell on piezoelectric fabric layer, myocardial cell arranges and growth along the length direction of the piezoelectric fabric in piezoelectric fabric layer; Two metal electrodes are connected electric capacity respectively, to obtain myocardial cell's generator; And removing sacrifice layer, and myocardial cell's generator is peeled off sheet glass.
The generating voltage of myocardial cell's generator and electric current by being prepared by aforesaid method measure, and electrical signal is more stable, and average voltage can reach 200mV, and mean current can reach 40nA.
In describing the invention, it will be appreciated that, term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ", " thickness ", " on ", D score, " front ", " afterwards ", " left side ", " right side ", " vertically ", " level ", " top ", " end " " interior ", " outward ", " clockwise ", " counterclockwise ", " axis ", " radial direction ", it is based on orientation shown in the drawings or position relation that the orientation of the instruction such as " circumference " or position are closed, it is only the present invention for convenience of description and simplified characterization, instead of indicate or imply that the device referred to or element must have specific orientation, with specific orientation structure and operation, therefore limitation of the present invention can not be interpreted as.
In the present invention, unless otherwise clearly defined and limited, the term such as term " installation ", " being connected ", " connection ", " fixing " should be interpreted broadly, such as, it is possible to be fixedly connected with, it is also possible to be removably connect, or integral; Can be mechanically connected, it is also possible to be electrical connection; Can be directly be connected, it is also possible to be indirectly connected by intermediary, it is possible to be the connection of two element internals or the interaction relationship of two elements. For the ordinary skill in the art, it is possible to understand above-mentioned term concrete implication in the present invention according to particular case.
In the present invention, unless otherwise clearly defined and limited, fisrt feature second feature " on " or D score can be that the first and second features directly contact, or the first and second features are by intermediary indirect contact. And, fisrt feature second feature " on ", " top " and " above " but fisrt feature directly over second feature or oblique upper, or only represent that fisrt feature level height is higher than second feature. Fisrt feature second feature " under ", " lower section " and " below " can be fisrt feature immediately below second feature or tiltedly lower section, or only represent that fisrt feature level height is less than second feature.
In the description of this specification sheets, at least one embodiment that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to be contained in the present invention in conjunction with concrete feature, structure, material or feature that this embodiment or example describe or example. In this manual, to the schematic representation of above-mentioned term need not for be identical embodiment or example. And, the concrete feature of description, structure, material or feature can combine in one or more embodiment in office or example in an appropriate manner. In addition, when not conflicting, the feature of the different embodiment described in this specification sheets or example and different embodiment or example can be carried out combining and combining by the technician of this area.
Although above it has been shown and described that embodiments of the invention, it is understandable that, above-described embodiment is exemplary, can not be interpreted as limitation of the present invention, and above-described embodiment can be changed, revises, replace and modification by the those of ordinary skill of this area within the scope of the invention.
Claims (7)
1. prepare the method for the myocardial cell's generator based on piezoelectric fabric for one kind, it is characterised in that, comprising:
Prepare one piece of sheet glass, and apply PNIPAM on the glass plates, to form sacrifice layer;
PDMS is applied, to form the PDMS film substrate of semicure on described sacrifice layer;
Half two metal electrodes are embedded respectively to the two ends on the PDMS film substrate of described semicure;
Forming piezoelectric fabric layer on the PDMS film substrate of semicure being partly embedded with metal electrode, the many piezoelectric fabric arragement directions forming described piezoelectric fabric layer unanimously and connect two described metal electrodes;
Uploading myocardial cell on described piezoelectric fabric layer, described myocardial cell arranges and growth along the length direction of the piezoelectric fabric in described piezoelectric fabric layer;
Described two metal electrodes are connected electric capacity respectively, to obtain described myocardial cell's generator; And
Remove described sacrifice layer, and described myocardial cell's generator is peeled off described sheet glass.
2. preparation according to claim 1 is based on the method for myocardial cell's generator of piezoelectric fabric, it is characterised in that, by adopting, high pressure far field electrospinning process prepares described piezoelectric fabric layer.
3. preparation according to claim 1 is based on the method for myocardial cell's generator of piezoelectric fabric, it is characterised in that, described piezoelectric fabric is prepared by any one in employing PZT, PCDF and zinc oxide.
4. preparation according to claim 2 is based on the method for myocardial cell's generator of piezoelectric fabric, it is characterized in that, described high pressure far field electrospinning process utilizes the external electric field that ground-electrode and impressed voltage are set up so that piezoelectric fabric falls along specific direction and arrangement.
5. preparation according to claim 1 is based on the method for myocardial cell's generator of piezoelectric fabric, it is characterised in that, before described piezoelectric fabric layer uploads myocardial cell, comprise further:
Use adhesion protein that described piezoelectric fabric layer is carried out surface modification, so that described Myocyte growth and sticking on described piezoelectric fabric layer.
6. one kind utilizes the myocardial cell's generator based on piezoelectric fabric that the method described in the arbitrary item of claim 1-5 prepares, it is characterised in that, described myocardial cell's generator based on piezoelectric fabric comprises:
PDMS film substrate;
Two electrodes, described two electrodes partly embed the upper surface of described PDMS film substrate respectively;
Piezoelectric fabric layer, described piezoelectric fabric layer load is at the upper surface of described PDMS film substrate, and described piezoelectric fabric layer is made up of the piezoelectric fabric that many arragement directions are consistent, and described piezoelectric fabric connects described two electrodes;
Myocardial cell's layer, described myocardial cell's layer load is on described piezoelectric fabric layer, and described myocardial cell's layer is made up of multiple myocardial cell, and myocardial cell described in each is along the length direction arrangement of described piezoelectric fabric and growth; And
Electric capacity, described electric capacity is connected with described two electrodes respectively.
7. the myocardial cell's generator based on piezoelectric fabric according to claim 6, it is characterised in that, described piezoelectric fabric is any one in PZT piezoelectric ceramic fibers, PCDF piezoelectric fabric and zinc oxide piezoelectric fiber.
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| CN201610122178.2A CN105633272A (en) | 2016-03-03 | 2016-03-03 | Piezoelectric fiber-based myocardial cell generator and preparation method thereof |
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| CN201610122178.2A CN105633272A (en) | 2016-03-03 | 2016-03-03 | Piezoelectric fiber-based myocardial cell generator and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1707934A (en) * | 2004-06-10 | 2005-12-14 | 米其林技术公司 | Piezoelectric ceramic fibers having metallic cores |
| US20120096955A1 (en) * | 2010-10-22 | 2012-04-26 | World Precision Instruments, Inc. | Combination Ultrasensitive Force Transducer and Grabbing Device for Force and Strain Measurement from Single Cells |
-
2016
- 2016-03-03 CN CN201610122178.2A patent/CN105633272A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1707934A (en) * | 2004-06-10 | 2005-12-14 | 米其林技术公司 | Piezoelectric ceramic fibers having metallic cores |
| US20120096955A1 (en) * | 2010-10-22 | 2012-04-26 | World Precision Instruments, Inc. | Combination Ultrasensitive Force Transducer and Grabbing Device for Force and Strain Measurement from Single Cells |
Non-Patent Citations (3)
| Title |
|---|
| T. ISHISAKA等: "BIO-ACTUATED POWER GENERATOR USING HEART MUSCLE CELLS ON A PDMS MEMBRANE", 《IEEE》 * |
| XIA LIU 等: "In Vitro cardiomyocyte-driven biogenerator based on aligned piezoelectric nanofibers", 《NANOSCALE》 * |
| XIA LIU，XIAOHONG WANG: "CARDIOMYOCYTES DRIVEN PIEZOELECTRIC NANOFIBER GENERATOR WITH ANISOTROPIC ENHANCEMENT", 《IEEE》 * |
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