CN105617425B - Ultrasonic sterilization verification method - Google Patents
Ultrasonic sterilization verification method Download PDFInfo
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- CN105617425B CN105617425B CN201510807664.3A CN201510807664A CN105617425B CN 105617425 B CN105617425 B CN 105617425B CN 201510807664 A CN201510807664 A CN 201510807664A CN 105617425 B CN105617425 B CN 105617425B
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 307
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 60
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000012795 verification Methods 0.000 title claims abstract description 13
- 241000607272 Vibrio parahaemolyticus Species 0.000 claims abstract description 20
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 10
- 230000005540 biological transmission Effects 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 6
- 230000008676 import Effects 0.000 claims description 3
- 210000004209 hair Anatomy 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 239000003599 detergent Substances 0.000 abstract description 6
- 238000005406 washing Methods 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000000369 enteropathogenic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 206010047400 Vibrio infections Diseases 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/12—Microwaves
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/14—Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/23—Containers, e.g. vials, bottles, syringes, mail
Landscapes
- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to bioengineering fields, and in particular to ultrasonic sterilization.Ultrasonic sterilization verification method, includes the following steps：The culture dish for being placed with vibrio parahemolyticus is put into a control box by step 1；Ultrasonic sterilization machine is put into control box by step 2；One temperature control system is packed into control box by step 3；Step 4 adjusts ultrasonic frequency, the temperature of time and temperature control system of ultrasonic sterilization machine；Step 5, by under a micro- sem observation different parameters, the content of vibrio parahemolyticus in culture dish.Step 6, the bactericidal effect that the content by observing vibrio parahemolyticus can obtain ultrasonic sterilization machine under which kind of environment are best.The present invention improves conventional washing agent sterilization mode, reduces washing process, substantially reduces detergent residual.The present invention more can accurately know that the content of interior vibrio parahemolyticus under which kind of parameter is minimum by the content of vibrio parahemolyticus under different parameters, and bactericidal effect is best.
Description
Technical field
The present invention relates to bioengineering fields, and in particular to ultrasonic sterilization.
Background technology
Nowadays, catering trade often uses detergent for the disinfection of tableware, once punching does not wash clean and is easy to stay on tableware
The residual of lower detergent also brings certain health hazard for the food being contained on tableware, and uses detergent mistake
Journey is cumbersome, needs to expend a large amount of manpower and materials, is not suitable for being used for a long time.
Often there is a kind of vibrio parahemolyticus on tableware, vibrio parahemolyticus is gram-Negative bacillus, in arcuation, bar
The various shapes such as shape, filiform, no gemma, food of the feed containing the bacterium can cause to poison by food, and also referred to as Halophiles is poisoned by food.Face
Using Acute onset, abdominal pain, vomiting, diarrhea and watery stool as cardinal symptom on bed.
Invention content
The object of the present invention is to provide a kind of ultrasonic sterilization verification methods, to solve the above technical problems.
Following technical scheme may be used to realize in technical problem solved by the invention：
Ultrasonic sterilization verification method, which is characterized in that include the following steps：
The culture dish for being placed with vibrio parahemolyticus is put into a control box by step 1；
Ultrasonic sterilization machine is put into control box by step 2；
One temperature control system is packed into control box by step 3；
Step 4 adjusts ultrasonic frequency, the temperature of time and temperature control system of ultrasonic sterilization machine；
Step 5, by under a micro- sem observation different parameters, the content of vibrio parahemolyticus in culture dish；
Step 6, the content by observing vibrio parahemolyticus can obtain the bactericidal effect of ultrasonic sterilization machine under which kind of environment
Most preferably.
The present invention improves conventional washing agent sterilization mode, reduces washing process, substantially reduces detergent residual.The present invention
It more can accurately know interior vibrio parahemolyticus under which kind of parameter by the content of vibrio parahemolyticus under different parameters
Content is minimum, and bactericidal effect is best.
The temperature control system includes a temperature sensor, and the temperature sensor connects a microprocessor system, described
Microprocessor system connects a master control set.
The present invention can record the temperature in control box by temperature sensor in real time.
The microprocessor connects an electronic display, and the electronic display is located at the outer wall of control box.
Temperature display in control box accurately can be come out personal observations easy to operation by the present invention by display screen.
The inner wall of the control box hangs with a clock module, and the clock module connects the microprocessor system.
The present invention can be recorded sterilizing time by clock module.
The master control set includes an illumination apparatus, and the illumination apparatus includes at least one led light-emitting components, described
The top that led light-emitting components are fixed on control box is located at the surface of the culture dish.
The present invention can be such that the temperature in control box increases by led light-emitting components by shining.
The master control set further includes an air-cooler, and the air-cooler is equipped with an air inlet and an air outlet, the outlet air
The ventilation opening of mouth attended operation box top, is equipped with a solenoid valve between the air inlet and the air outlet, the solenoid valve connects
Connect the microprocessor system.
The present invention is more enough to control air-cooler according to the height of temperature, is reduced to the temperature in control box.
Scheme one, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 100Hz~200Hz, and for sterilizing time in 0s~5s, sterilizing rate in the culture dish is 30%, when sterilization
Between in 5s~10s, sterilizing rate in the culture dish is 40%~45%, and sterilizing time is in 10s~15s, the culture
Sterilizing rate in ware is 45%~50%, sterilizing time in 15s~20s, the sterilizing rate in the culture dish is 50%~
55%, when the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 50%~55%.
The present invention is by scheme one, at 10 DEG C~20 DEG C of temperature, the parameter of ultrasonic frequency 100Hz~200Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 50%~55%.
Scheme two, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 200Hz~300Hz, and for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 43%~48%,
For sterilizing time in 5s~10s, the sterilizing rate in the culture dish is 48%~55%, and sterilizing time is in 10s~15s, institute
The sterilizing rate stated in culture dish is 55%~60%, and in 15s~20s, the sterilizing rate in the culture dish is sterilizing time
60%~65%, when the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 60%~65%.
The present invention is by scheme two, at 10 DEG C~20 DEG C of temperature, the parameter of ultrasonic frequency 200Hz~300Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 60%~65%.
Scheme three, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 400Hz~500Hz, and for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 52%~58%,
For sterilizing time in 5s~10s, the sterilizing rate in the culture dish is 58%~63%, and sterilizing time is in 10s~15s, institute
The sterilizing rate stated in culture dish is 63%~71%, and in 15s~20s, the sterilizing rate in the culture dish is sterilizing time
71%~76%, when the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 71%~76%.
The present invention is by scheme three, at 10 DEG C~20 DEG C of temperature, the parameter of ultrasonic frequency 400Hz~500Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 71%~76%.
Scheme four, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 500Hz~600Hz, and for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 62%~68%,
For sterilizing time in 5s~10s, the sterilizing rate in the culture dish is 68%~73%, and sterilizing time is in 10s~15s, institute
The sterilizing rate stated in culture dish is 73%~81%, and in 15s~20s, the sterilizing rate in the culture dish is sterilizing time
81%~86%, when the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 81%~86%.
The present invention is by scheme four, at 10 DEG C~20 DEG C of temperature, the parameter of ultrasonic frequency 500Hz~600Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 81%~86%.
Scheme five, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 600Hz~700Hz, and for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 62%~68%,
For sterilizing time in 5s~10s, the sterilizing rate in the culture dish is 68%~73%, and sterilizing time is in 10s~15s, institute
The sterilizing rate stated in culture dish is 73%~81%, and in 15s~20s, the sterilizing rate in the culture dish is sterilizing time
81%~86%, when the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 81%~86%.
The present invention is by scheme five, and when ultrasonic frequency is between 600Hz~700Hz, sterilizing rate exists with ultrasonic frequency
It is identical between 500Hz~600Hz.The present invention can obtain ultrasonic frequency by one~scheme of scheme five, according to data statistics and exist
Sterilizing rate highest between 500Hz~600Hz.
The ultrasonic frequency of ultrasonic sterilization machine is maintained at by scheme six when the temperature of control box is at 20 DEG C~30 DEG C
Between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 65%~70%, sterilizing time
In 5s~10s, the sterilizing rate in the culture dish is 70%~78%, and sterilizing time is in 10s~15s, the culture dish
Interior sterilizing rate is 78%~85%, and for sterilizing time in 15s~20s, the sterilizing rate in the culture dish is 85%~90%,
When the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 85%~90%.
The present invention is by scheme six, at 20 DEG C~30 DEG C of temperature, the parameter of ultrasonic frequency 500Hz~600Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 85%~90%.
The ultrasonic frequency of ultrasonic sterilization machine is maintained at by scheme seven when the temperature of control box is at 30 DEG C~40 DEG C
Between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 70%~75%, sterilizing time
In 5s~10s, the sterilizing rate in the culture dish is 75%~80%, and sterilizing time is in 10s~15s, the culture dish
Interior sterilizing rate is 80%~88%, and for sterilizing time in 15s~20s, the sterilizing rate in the culture dish is 88%~97%,
When the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 88%~97%.
The present invention is by scheme seven, at 30 DEG C~40 DEG C of temperature, the parameter of ultrasonic frequency 500Hz~600Hz, culture
The sterilizing rate of ware is improved with the extension of time, but sterilizing rate peak rests on 88%~97%.
The ultrasonic frequency of ultrasonic sterilization machine is maintained at by scheme eight when the temperature of control box is at 40 DEG C~50 DEG C
Between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in the culture dish is 70%~75%, sterilizing time
In 5s~10s, the sterilizing rate in the culture dish is 75%~80%, and sterilizing time is in 10s~15s, the culture dish
Interior sterilizing rate is 80%~88%, and for sterilizing time in 15s~20s, the sterilizing rate in the culture dish is 88%~97%,
When the sterilizing time is more than 20s, the sterilizing rate in the culture dish is 88%~97%.
The present invention is by scheme eight, at 40 DEG C~50 DEG C of temperature, the parameter of ultrasonic frequency 500Hz~600Hz, culture
The sterilizing rate of ware and temperature are consistent at 30 DEG C~40 DEG C.The present invention can be obtained by six~scheme of scheme eight, temperature 30 DEG C~
40 DEG C of sterilizing rate highests.
As a kind of scheme, when temperature is at 30 DEG C~40 DEG C, ultrasonic frequency is 500Hz~600Hz, and sterilizing time exists
When 15s~20s, the sterilizing rate in the culture dish is 88%~97%.
For the present invention by one~scheme of scheme eight, it is 30 DEG C~40 DEG C of temperature that can obtain parameter, ultrasonic frequency be 500Hz~
600Hz, sterilizing time bactericidal effect in 15s~20s are best.
As a preferred embodiment, when temperature is 35 DEG C, ultrasonic frequency 550Hz, sterilizing time 15s are described
Sterilizing rate in culture dish is 95%.
As another preferred embodiment, when temperature is 37 DEG C, ultrasonic frequency 550Hz, sterilizing time 15s, institute
It is 97% to state the sterilizing rate in culture dish.
For the present invention when temperature is 37 DEG C, bactericidal effect is best.
Description of the drawings
Fig. 1 is the flow diagram of the present invention.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Conjunction is specifically illustrating that the present invention is further explained.
Referring to Fig.1, ultrasonic sterilization verification method includes the following steps：Step 1 will be placed with vibrio parahemolyticus
Culture dish is put into a control box；Ultrasonic sterilization machine is put into control box by step 2；Step 3 fills a temperature control system
Enter in control box；Step 4 adjusts ultrasonic frequency, the temperature of time and temperature control system of ultrasonic sterilization machine；Step 5 is led to
It crosses under a micro- sem observation different parameters, the content of vibrio parahemolyticus in culture dish.Step 6, by observing parahemolyticas arc
The bactericidal effect that the content of bacterium can obtain ultrasonic sterilization machine under which kind of environment is best.The present invention improves conventional washing agent sterilization mould
Formula reduces washing process, substantially reduces detergent residual.The present invention can by the content of vibrio parahemolyticus under different parameters
More accurately know that the content of interior vibrio parahemolyticus under which kind of parameter is minimum, bactericidal effect is best.Temperature control system includes one
Temperature sensor, temperature sensor connect a microprocessor system, and microprocessor system connects a master control set.The present invention
The temperature in control box can be recorded in real time by temperature sensor.Microprocessor connects an electronic display, electronical display
Outer wall of the screen positioned at control box.The present invention can be accurately out easy to operation by the temperature display in control box by display screen
Personal observations.The inner wall of control box hangs with a clock module, and clock module connects microprocessor system.The present invention passes through clock
Module can record sterilizing time.Master control set includes an illumination apparatus, and illumination apparatus includes that at least one led shines
Element, the top that led light-emitting components are fixed on control box are located at the surface of culture dish.The present invention passes through led light-emitting component energy
It is enough so that the temperature in control box is increased by shining.
Master control set further includes an air-cooler, and air-cooler is equipped with an air inlet and an air outlet, air outlet attended operation case
The ventilation opening at top, is equipped with a solenoid valve between air inlet and air outlet, solenoid valve connects microprocessor system.The present invention is more
It is enough that air-cooler is controlled according to the height of temperature, the temperature in control box is reduced.As a kind of scheme, include in culture dish
Salmonella, staphylococcus aureus, vibrio parahemolyticus, Bacillus cereus, enteropathogenic E. Coli and perfringens
Bacillus.When temperature is 45 DEG C, ultrasonic frequency 480Hz, sterilizing time 20s, the Salmonella sterilization in culture dish
Rate is 96%.When temperature is 32 DEG C, ultrasonic frequency 580Hz, sterilizing time 35s, the golden yellow grape in culture dish
Coccus sterilizing rate is 98%.When temperature is 35 DEG C, ultrasonic frequency 450Hz, sterilizing time 20s, the wax in culture dish
Sample bacillus sterilizing rate is 97%.When temperature is 40 DEG C, ultrasonic frequency 520Hz, sterilizing time 25s, culture dish
Interior enteropathogenic E. Coli sterilizing rate is 96%.When temperature is 36 DEG C, ultrasonic frequency 500Hz, sterilizing time is
30s, the Bacillus perfringens sterilizing rate in culture dish are 97%.As a preferred embodiment, include parahemolyticas in culture dish
Vibrios.
Scheme one, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 100Hz~200Hz, and for sterilizing time in 0s~5s, the sterilizing rate in culture dish is 30%, and sterilizing time exists
When 5s~10s, the sterilizing rate in culture dish is 40%~45%, and sterilizing time is in 10s~15s, the sterilizing rate in culture dish
It is 45%~50%, for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 50%~55%, and sterilizing time is more than
When 20s, the sterilizing rate in culture dish is 50%~55%.The present invention is by scheme one, in 10 DEG C~20 DEG C of temperature, ultrasonic wave frequency
Under the parameter of rate 100Hz~200Hz, the sterilizing rate of culture dish is improved with the extension of time, but sterilizing rate peak stops
Stay in 50%~55%.Scheme two, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the super of ultrasonic sterilization machine
Frequency of sound wave adjusts between 200Hz~300Hz, sterilizing time in 0s~5s, sterilizing rate in culture dish is 43%~
48%, for sterilizing time in 5s~10s, the sterilizing rate in culture dish is 48%~55%, and sterilizing time is in 10s~15s, training
It is 55%~60% to support the sterilizing rate in ware, and for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 60%~65%,
When sterilizing time is more than 20s, the sterilizing rate in culture dish is 60%~65%.The present invention by scheme two, 10 DEG C of temperature~
20 DEG C, under the parameter of ultrasonic frequency 200Hz~300Hz, the sterilizing rate of culture dish is improved with the extension of time, but is killed
Bacterium rate peak rests on 60%~65%.Scheme three will be ultrasonic when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C
The ultrasonic frequency of wave sterilization machine adjusts between 400Hz~500Hz, and sterilizing time is in 0s~5s, the sterilization in culture dish
Rate is 52%~58%, and for sterilizing time in 5s~10s, the sterilizing rate in culture dish is 58%~63%, and sterilizing time is in 10s
When~15s, the sterilizing rate in culture dish is 63%~71%, and in 15s~20s, the sterilizing rate in culture dish is sterilizing time
71%~76%, when sterilizing time is more than 20s, the sterilizing rate in culture dish is 71%~76%.The present invention by scheme three,
10 DEG C~20 DEG C of temperature, under the parameter of ultrasonic frequency 400Hz~500Hz, the sterilizing rate of culture dish with the extension of time and
It improves, but sterilizing rate peak rests on 71%~76%.
Scheme four, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by the ultrasonic wave frequency of ultrasonic sterilization machine
Rate adjusts between 500Hz~600Hz, and for sterilizing time in 0s~5s, the sterilizing rate in culture dish is 62%~68%, sterilization
Time, the sterilizing rate in culture dish was 68%~73%, and sterilizing time is in 10s~15s, in culture dish in 5s~10s
Sterilizing rate is 73%~81%, and for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 81%~86%, sterilizing time
When more than 20s, the sterilizing rate in culture dish is 81%~86%.The present invention is by scheme four, in 10 DEG C~20 DEG C of temperature, ultrasound
Under the parameter of wave frequency rate 500Hz~600Hz, the sterilizing rate of culture dish is improved with the extension of time, but sterilizing rate highest
Value rests on 81%~86%.Scheme five, when the temperature of control box is when remaining unchanged for 10 DEG C~20 DEG C, by ultrasonic sterilization machine
Ultrasonic frequency adjust between 600Hz~700Hz, for sterilizing time in 0s~5s, sterilizing rate in culture dish is 62%
~68%, for sterilizing time in 5s~10s, sterilizing rate in culture dish is 68%~73%, sterilizing time in 10s~15s,
Sterilizing rate in culture dish is 73%~81%, sterilizing time in 15s~20s, the sterilizing rate in culture dish is 81%~
86%, when sterilizing time is more than 20s, the sterilizing rate in culture dish is 81%~86%.The present invention passes through scheme five, ultrasonic wave frequency
When rate is between 600Hz~700Hz, sterilizing rate is identical between 500Hz~600Hz as ultrasonic frequency.The present invention side of passing through
One~scheme of case five can obtain ultrasonic frequency sterilizing rate highest between 500Hz~600Hz according to data statistics.
The ultrasonic frequency of ultrasonic sterilization machine is maintained at by scheme six when the temperature of control box is at 20 DEG C~30 DEG C
Between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in culture dish is 65%~70%, and sterilizing time is in 5s
When~10s, the sterilizing rate in culture dish is 70%~78%, and in 10s~15s, the sterilizing rate in culture dish is sterilizing time
78%~85%, for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 85%~90%, and sterilizing time is more than 20s
When, the sterilizing rate in culture dish is 85%~90%.The present invention is by scheme six, in 20 DEG C~30 DEG C of temperature, ultrasonic frequency
Under the parameter of 500Hz~600Hz, the sterilizing rate of culture dish is improved with the extension of time, but sterilizing rate peak stops
85%~90%.Scheme seven protects the ultrasonic frequency of ultrasonic sterilization machine when the temperature of control box is at 30 DEG C~40 DEG C
It holds between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in culture dish is 70%~75%, sterilizing time
In 5s~10s, the sterilizing rate in culture dish is 75%~80%, and sterilizing time is in 10s~15s, the sterilization in culture dish
Rate is 80%~88%, and for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 88%~97%, and sterilizing time is more than
When 20s, the sterilizing rate in culture dish is 88%~97%.The present invention is by scheme seven, in 30 DEG C~40 DEG C of temperature, ultrasonic wave frequency
Under the parameter of rate 500Hz~600Hz, the sterilizing rate of culture dish is improved with the extension of time, but sterilizing rate peak stops
Stay in 88%~97%.
The ultrasonic frequency of ultrasonic sterilization machine is maintained at by scheme eight when the temperature of control box is at 40 DEG C~50 DEG C
Between 500Hz~600Hz, for sterilizing time in 0s~5s, the sterilizing rate in culture dish is 70%~75%, and sterilizing time is in 5s
When~10s, the sterilizing rate in culture dish is 75%~80%, and in 10s~15s, the sterilizing rate in culture dish is sterilizing time
80%~88%, for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 88%~97%, and sterilizing time is more than 20s
When, the sterilizing rate in culture dish is 88%~97%.The present invention is by scheme eight, in 40 DEG C~50 DEG C of temperature, ultrasonic frequency
Under the parameter of 500Hz~600Hz, the sterilizing rate of culture dish and temperature are consistent at 30 DEG C~40 DEG C.The present invention passes through scheme six
~scheme eight can obtain, and temperature is in 30 DEG C~40 DEG C sterilizing rate highests.As a kind of scheme, when temperature is at 30 DEG C~40 DEG C, surpass
Frequency of sound wave is 500Hz~600Hz, and for sterilizing time in 15s~20s, the sterilizing rate in culture dish is 88%~97%.This hair
Bright it is 30 DEG C~40 DEG C of temperature that can obtain parameter by one~scheme of scheme eight, and ultrasonic frequency is 500Hz~600Hz, when sterilization
Between in 15s~20s bactericidal effect it is best.As a preferred embodiment, when temperature is 35 DEG C, ultrasonic frequency 550Hz,
Sterilizing time is 15s, and the sterilizing rate in culture dish is 95%.As another preferred embodiment, when temperature is 37 DEG C, ultrasonic wave
Frequency is 550Hz, sterilizing time 15s, and the sterilizing rate in culture dish is 97%.The present invention is when temperature is 37 DEG C, sterilization effect
Fruit is best.It is equipped with a culture dish import in control box and a culture dish exports, culture dish outlet passes through a transmission track and culture
Ware import, transmission track are connect with a transmission device, and transmission device connects a motor, and motor is fixed on the outer of control box
Side.The present invention can reduce artificial culture dish holding by transmission track can bring certain bacterium, pollution culture dish to influence examination
Test data.Motor connects microprocessor system.The present invention can control motor every one by microprocessor system
The section time makes transmission track operate.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (7)
1. ultrasonic sterilization verification method, including a nozzle body, which is characterized in that include the following steps：
The culture dish for being placed with vibrio parahemolyticus is put into a control box by step 1；
Ultrasonic sterilization machine is put into control box by step 2；
One temperature control system is packed into control box by step 3；
Step 4 adjusts ultrasonic frequency, the temperature of time and temperature control system of ultrasonic sterilization machine；
Step 5, by under a micro- sem observation different parameters, the content of vibrio parahemolyticus in culture dish；
Step 6, the content by observing vibrio parahemolyticus can obtain the bactericidal effect of ultrasonic sterilization machine under which kind of environment most
It is good；
The temperature control system includes a temperature sensor, and the temperature sensor connects a microprocessor system, described miniature
Processor system connects a master control set；
The master control set includes an illumination apparatus, and the illumination apparatus includes at least one led light-emitting components, the led hairs
The top that optical element is fixed on control box is located at the surface of the culture dish；
Be equipped with a culture dish import in control box and a culture dish export, culture dish outlet by a transmission track and culture dish into
Mouthful, transmission track is connect with a transmission device, and transmission device connects a motor, and motor is fixed on the outside of control box；
Motor connects microprocessor system；Can control motor by microprocessor system makes biography at regular intervals
Track is sent to operate.
2. ultrasonic sterilization verification method according to claim 1, it is characterised in that：One electricity of microprocessor connection
Sub- display screen, the electronic display are located at the outer wall of control box.
3. ultrasonic sterilization verification method according to claim 1, it is characterised in that：When control box temperature 10 DEG C~
20 DEG C when remaining unchanged, the ultrasonic frequency of ultrasonic sterilization machine are adjusted between 100Hz~200Hz, sterilizing time is in 0s
When~5s, the sterilizing rate in the culture dish is 30%, and in 5s~10s, the sterilizing rate in the culture dish is sterilizing time
40%~45%, for sterilizing time in 10s~15s, the sterilizing rate in the culture dish is 45%~50%, and sterilizing time exists
When 15s~20s, the sterilizing rate in the culture dish is 50%~55%, when the sterilizing time is more than 20s, the culture dish
Interior sterilizing rate is 50%~55%.
4. ultrasonic sterilization verification method according to claim 1, it is characterised in that：When control box temperature 10 DEG C~
20 DEG C when remaining unchanged, the ultrasonic frequency of ultrasonic sterilization machine are adjusted between 200Hz~300Hz, sterilizing time is in 0s
When~5s, the sterilizing rate in the culture dish is 43%~48%, and sterilizing time is in 5s~10s, killing in the culture dish
Bacterium rate is 48%~55%, and for sterilizing time in 10s~15s, sterilizing rate in the culture dish is 55%~60%, when sterilization
Between in 15s~20s, sterilizing rate in the culture dish is 60%~65%, when the sterilizing time is more than 20s, the training
It is 60%~65% to support the sterilizing rate in ware.
5. ultrasonic sterilization verification method according to claim 1, it is characterised in that：When control box temperature 10 DEG C~
20 DEG C when remaining unchanged, the ultrasonic frequency of ultrasonic sterilization machine are adjusted between 500Hz~600Hz, sterilizing time is in 0s
When~5s, the sterilizing rate in the culture dish is 62%~68%, and sterilizing time is in 5s~10s, killing in the culture dish
Bacterium rate is 68%~73%, and for sterilizing time in 10s~15s, sterilizing rate in the culture dish is 73%~81%, when sterilization
Between in 15s~20s, sterilizing rate in the culture dish is 81%~86%, when the sterilizing time is more than 20s, the training
It is 81%~86% to support the sterilizing rate in ware.
6. ultrasonic sterilization verification method according to claim 1, it is characterised in that：When control box temperature 30 DEG C~
At 40 DEG C, between the ultrasonic frequency of ultrasonic sterilization machine is maintained at 500Hz~600Hz, sterilizing time is in 0s~5s, institute
It is 70%~75% to state the sterilizing rate in culture dish, and for sterilizing time in 5s~10s, the sterilizing rate in the culture dish is 75%
~80%, for sterilizing time in 10s~15s, sterilizing rate in the culture dish is 80%~88%, sterilizing time 15s~
When 20s, the sterilizing rate in the culture dish is 88%~97%, when the sterilizing time is more than 20s, killing in the culture dish
Bacterium rate is 88%~97%.
7. ultrasonic sterilization verification method according to claim 6, it is characterised in that：When temperature is 37 DEG C, ultrasonic wave
Frequency is 550Hz, sterilizing time 15s, and the sterilizing rate in the culture dish is 97%.
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| CN201510807664.3A CN105617425B (en) | 2015-11-19 | 2015-11-19 | Ultrasonic sterilization verification method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4839142A (en) * | 1985-09-30 | 1989-06-13 | Charm Stanley E | High temperature, short time heating system and method of sterilizing or pasteurizing heat sensitive biological fluids |
| CN103926189A (en) * | 2014-04-25 | 2014-07-16 | 中国科学院南京地理与湖泊研究所 | Flow-cytometry-based method for rapidly measuring heterotrophic bacteria in eutrophic lake |
-
2015
- 2015-11-19 CN CN201510807664.3A patent/CN105617425B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4839142A (en) * | 1985-09-30 | 1989-06-13 | Charm Stanley E | High temperature, short time heating system and method of sterilizing or pasteurizing heat sensitive biological fluids |
| CN103926189A (en) * | 2014-04-25 | 2014-07-16 | 中国科学院南京地理与湖泊研究所 | Flow-cytometry-based method for rapidly measuring heterotrophic bacteria in eutrophic lake |
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Denomination of invention: Verification method for ultrasonic sterilization Granted publication date: 20180720 Pledgee: Minhang Branch of Shanghai Rural Commercial Bank Co.,Ltd. Pledgor: INGLE DETECTION TECHNOLOGY SERVICES (SHANGHAI) CO.,LTD. Registration number: Y2024310000058 |
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Denomination of invention: Verification method for ultrasonic sterilization Granted publication date: 20180720 Pledgee: Bank of Shanghai Limited by Share Ltd. Minhang branch Pledgor: INGLE DETECTION TECHNOLOGY SERVICES (SHANGHAI) CO.,LTD. Registration number: Y2024980012870 |