CN105616511A

CN105616511A - Bagged tea capable of preventing and treating prostate disease and preparation method thereof

Info

Publication number: CN105616511A
Application number: CN201511013686.9A
Authority: CN
Inventors: 霍云宏; 苗明三; 王言和
Original assignee: Henan Hongye Biotechnology Co Ltd
Current assignee: Henan Hongye Biotechnology Co Ltd
Priority date: 2015-12-31
Filing date: 2015-12-31
Publication date: 2016-06-01

Abstract

The invention discloses bagged tea capable of preventing and treating a prostate disease. The bagged tea is prepared from, by weight, 40-60 parts of flowers of hibiscus manihot L., 20-40 parts of seed powder of hibiscus manihot L., 20-40 parts of dandelions, 20-40 parts of longhairy antenoron herb and 5-15 parts of jasmine flowers. The bagged tea can effectively improve and prevent prostatic lesions, has the significant preventing and treating effect on symptoms such as prostatic hyperplasia, prostatitis and prostatic hypertrophy, is free of toxicity, harm and side effect and has no harm to human prostate tissue, kidney tissue, testicular tissue and the like. Hibiscus manihot L. is prepared into the bagged tea, the preparation method is simple, the loss of the effective ingredients is less, the leaching rate is high, the leached medicinal ingredients are more, the curative effect is significant, drinking is convenient, and tea water is uniform in leaching, transparent in color and luster and aromatic in smell.

Description

A kind of can the teabag of prevention and therapy prostatosis and its preparation method

Technical field

The invention belongs to healthcare products preparation field, be specifically related to a kind of can the teabag of prevention and therapy prostatosis and its preparation method.

Background technology

Hibiscus manihot L. is annual herb Malvaceae Abelmoschus plant. Fresh flower contains multiple biologically active substance, its biological flavone content is up to 6%, containing full price protein, high glycan glue, food fibre, trace element-selenium, zinc, multiple unsaponifiables etc., having and regulate human endocrine, immunizing power, increase human body is anti-resists aging, anti-cancer, blood fat reducing function strenuously. In addition, its abundant natural phant oestrogenic hormon, can extend women's pubescence, alleviate person in middle and old age's climacteric syndrome. Flower can immediate access be eaten raw, cold and dressed with sauce, to propagandize, cook soup or mix wheaten food hotly edible, or to spend soaked generation tea etc. Its fresh flower entrance descendant has a kind of strange, fresh, refreshing, fragrant, relaxed and joyful impression, has keeping tensions down mood, function of adjusting mentality. Tender fruit has the effect identical with okra, can fry, cook soup, eat something rare. Flower of JINHUAKUI dietotherapeutic, Compendium of Material Medica has recorded the pharmaceutical use of Sunset Abelmoschus Root, energy heat-clearing, cool blood, removing toxic substances, and it spends nontoxic, has effect of eliminating damp-heat, anti-inflammatory analgesic, takes orally and cures mainly five pouring, oedema, and external curing soup is scalded. Fruit, seed have tonifying spleen stomach invigorating, myogenic effect, treatment maldigestion, anorexia, wound etc. " good help book on Chinese herbal medicine " is recorded: it is dripping and expedite the emergence of, control all evil sores that Flower of Sunset Abelmoschus cures mainly urine. Modern medicine study shows, the total flavones of this flower has the effects such as analgesia, anti-cerebral ischemia and treatment stomatocace. Full price protein, high glycan glue, food fibre, trace element-selenium, zinc, multiple unsaponifiables etc. contained by it, have and significantly regulate human endocrine, immunizing power, increase the anti-power of human body, improve cardiovascular and cerebrovascular and microcirculation function, enhancing body resistance of oxidation, anti-cardiac-cerebral ischemia, anoxic, anti-inflammation, town pain, antifatigue, cancer anti-ageing, anticancer, anti-, blood fat reducing function. In addition, its abundant natural phant oestrogenic hormon, for extending women's pubescence, alleviates or avoids person in middle and old age's climacteric syndrome also to have special effect. Hibiscus manihot L. is also containing abundant Quercetin 3-galactoside composition, and Quercetin 3-galactoside has stronger antitussive action, assimilation, in addition, also has the effect of stronger suppression eye aldose reductase.

Summary of the invention

It is an object of the invention to provide a kind of can the teabag of prevention and therapy prostatosis and its preparation method, this teabag can effectively improve and prevent prostatic lesion, the diseases such as hyperplasia of prostate, prostatitis and prostatomegaly had significant prevention effect, and it is nontoxic, have no side effect, to tissues such as human prostate tissues, renal tissue, testis without any injury.

The technical solution adopted in the present invention is: a kind of can the teabag of prevention and therapy prostatosis, be made up of following bulk drug according to weight part number: flower of JINHUAKUI 40-60 part, JINHUAKUIZI powder 20-40 part, taraxacum 20-40 part, Longhairy Antenoron Herb 20-40 part and Flower of Arabian Jasmine 5-15 part.

Described can the preparation method of teabag of prevention and therapy prostatosis, comprise the following steps:

Step one, getting flower of JINHUAKUI raw material, be placed in steam oven, leading to is the steam insulation 30-50min of 110 DEG C into temperature, then 120 DEG C it are warming up to, insulation 10min, transfers in the thermostat container that temperature is 25 DEG C, keeps 10-20min, take out, being placed in baking oven to be dried, the temperature in baking oven is 60-70 DEG C, and time of drying is 90-120min, obtain flower of JINHUAKUI bulk drug, for subsequent use;

Step 2, get seed of JINHUAKUI raw material, first carry out coarse reduction with pulverizer, then it is placed in pressure cooker, 5-10min is steamed when 1-2Mpa, take out while hot after having steamed and it is placed in sealed can interior sealing more than 3h, then it is placed in baking oven to be dried and be no more than 10% to moisture content, obtains JINHUAKUIZI powder, for subsequent use;

Step 3, get bulk drug flower of JINHUAKUI 40-60 part according to weight part number, JINHUAKUIZI powder 20-40 part, taraxacum 20-40 part, Longhairy Antenoron Herb 20-40 part and Flower of Arabian Jasmine 5-15 part, first tree mallow, JINHUAKUIZI powder, taraxacum and Longhairy Antenoron Herb mixing are placed in supper micron mill, it is crushed to 200 orders, then take out and it is placed in iron pot, slow fire frying 2-10min, frying adds Flower of Arabian Jasmine 5-15 part while hot after terminating, mix, carrying out being distributed into bag, namely completing can the preparation of teabag of prevention and therapy prostatosis again.

As the present invention a kind of can the further optimization of preparation method of teabag of prevention and therapy prostatosis, the flower of JINHUAKUI starting material described in step one before being placed in steam oven first with the warm water soaking 20-60min that temperature is 40-50 DEG C.

As the present invention is a kind of can the further optimization of preparation method of teabag of prevention and therapy prostatosis, described in step 3, the concocting method of Longhairy Antenoron Herb bulk drug is: get Longhairy Antenoron Herb raw material, it is placed in steam oven, leading to is the steam insulation 45min of 110 DEG C into temperature, then 120 DEG C it are warming up to, lead to and make air pressure inside reach 1.5MPa into nitrogen, Longhairy Antenoron Herb starting material in abrupt release steam oven after lasting 5min, transfer in the thermostat container that temperature is 30 DEG C, insulation 30min, take out, it is placed in baking oven to be dried, it is no more than 10% to moisture content, namely Longhairy Antenoron Herb bulk drug is obtained.

Compared with prior art, the present invention at least has following advantage and useful effect:

The flower of JINHUAKUI teabag preventing and treating prostatosis provided by the invention can effectively improve and prevent prostatic lesion, the diseases such as hyperplasia of prostate, prostatitis and prostatomegaly had significant prevention effect, adhere to taking and can significantly reduce hyperplasia of prostate index, reduce serum DHT, T level, and can remarkable increasing serum E2 level. For prostatitis, it is possible to significantly reduce leukocyte count in prostata tissue, significantly increase lecithin density, significantly alleviate the pathological change of prostate gland, epididymis tissue. The teabag of the present invention is remarkable for the prevention and therapy effect of prostatosis, and nontoxic, has no side effect, to tissues such as human prostate tissues, renal tissue, testis without any injury.

Hibiscus manihot L. is made into the form of teabag by the present invention, and preparation method is simple, and loss of effective components is few, leaching yield height, and the effective component of leaching is many, evident in efficacy, easy administration, and tea leaches evenly, and color and luster is penetrating, smell fragrance.

Accompanying drawing explanation

Fig. 1 is that prostatic hyperplasia model mouse prostate tissue morphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 2 is that prostatic hyperplasia model mouse thymus tissue morphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 3 is that prostatic hyperplasia model mouse spleen tissue morphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 4 is that flower of JINHUAKUI is to prostatic hyperplasia model mouse kidney tissue morphology influence comparison diagram;

Fig. 5 is that prostatic hyperplasia model mouse testis tissue morphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 6 is that prostatic hyperplasia model mouse epididymis tissue morphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 7 is that prostatitis model mice prostatic histomorphology is affected comparison diagram by flower of JINHUAKUI;

Fig. 8 is that flower of JINHUAKUI is to prostatitis model mice renal tissue morphology influence comparison diagram;

Fig. 9 is that flower of JINHUAKUI is to prostatitis model mice testis tissue morphology influence comparison diagram;

Figure 10 is that prostatitis model mice epididymis tissue morphology is affected comparison diagram by flower of JINHUAKUI.

Embodiment

For making the content of the present invention become apparent, below in conjunction with specific embodiment, describe the present invention.

Can the teabag of prevention and therapy prostatosis, be made up of following bulk drug according to weight part number: flower of JINHUAKUI 40-60 part, JINHUAKUIZI powder 20-40 part, taraxacum 20-40 part, Longhairy Antenoron Herb 20-40 part and Flower of Arabian Jasmine 5-15 part.

In order to make the present invention have better implementation result, the flower of JINHUAKUI starting material described in step one before being placed in steam oven first with the warm water soaking 20-60min that temperature is 40-50 DEG C.

In technical solution of the present invention, described in step 3, the concocting method of Longhairy Antenoron Herb bulk drug is: get Longhairy Antenoron Herb raw material, is placed in steam oven, leading to is the steam insulation 45min of 110 DEG C into temperature, then it is warming up to 120 DEG C, leads to and make air pressure inside reach 1.5MPa into nitrogen, the Longhairy Antenoron Herb starting material in abrupt release steam oven after lasting 5min, transfer in the thermostat container that temperature is 30 DEG C, insulation 30min, takes out, is placed in baking oven and is dried, it is no more than 10% to moisture content, namely obtains Longhairy Antenoron Herb bulk drug.

Embodiment 1:

Can the teabag of prevention and therapy prostatosis, be made up of following bulk drug according to weight part number: flower of JINHUAKUI 40 parts, 20 parts, JINHUAKUIZI powder, taraxacum 20 parts, Longhairy Antenoron Herb 20 parts and Flower of Arabian Jasmine 5 parts.

Step one, getting flower of JINHUAKUI raw material, be placed in steam oven, leading to is the steam insulation 30min of 110 DEG C into temperature, then 120 DEG C it are warming up to, insulation 10min, transfers in the thermostat container that temperature is 25 DEG C, keeps 10min, take out, being placed in baking oven to be dried, the temperature in baking oven is 60-70 DEG C, and time of drying is 90min, obtain flower of JINHUAKUI bulk drug, for subsequent use;

Step 2, get seed of JINHUAKUI raw material, first carry out coarse reduction with pulverizer, then it is placed in pressure cooker, 5min is steamed when 1Mpa, take out while hot after having steamed and it is placed in sealed can interior sealing more than 3h, then it is placed in baking oven to be dried and be no more than 10% to moisture content, obtains JINHUAKUIZI powder, for subsequent use;

Step 3, get bulk drug according to weight part number in above-mentioned formula, first tree mallow, JINHUAKUIZI powder, taraxacum and Longhairy Antenoron Herb mixing are placed in supper micron mill, it is crushed to 200 orders, then take out and it is placed in iron pot, slow fire frying 2min, frying adds Flower of Arabian Jasmine while hot after terminating, mix, carrying out being distributed into bag, namely completing can the preparation of teabag of prevention and therapy prostatosis again.

Embodiment 2:

Can the teabag of prevention and therapy prostatosis, be made up of following bulk drug according to weight part number: flower of JINHUAKUI 60 parts, 40 parts, JINHUAKUIZI powder, taraxacum 40 parts, Longhairy Antenoron Herb 40 parts and Flower of Arabian Jasmine 15 parts.

Step one, getting flower of JINHUAKUI raw material, be placed in steam oven, leading to is the steam insulation 50min of 110 DEG C into temperature, then 120 DEG C it are warming up to, insulation 10min, transfers in the thermostat container that temperature is 25 DEG C, keeps 20min, take out, being placed in baking oven to be dried, the temperature in baking oven is 60-70 DEG C, and time of drying is 120min, obtain flower of JINHUAKUI bulk drug, for subsequent use;

Step 2, get seed of JINHUAKUI raw material, first carry out coarse reduction with pulverizer, then it is placed in pressure cooker, 10min is steamed when 2Mpa, take out while hot after having steamed and it is placed in sealed can interior sealing more than 3h, then it is placed in baking oven to be dried and be no more than 10% to moisture content, obtains JINHUAKUIZI powder, for subsequent use;

Step 3, get bulk drug according to the weight part number in above-mentioned formula, first tree mallow, JINHUAKUIZI powder, taraxacum and Longhairy Antenoron Herb mixing are placed in supper micron mill, it is crushed to 200 orders, then take out and it is placed in iron pot, slow fire frying 9min, frying adds Flower of Arabian Jasmine while hot after terminating, mix, carrying out being distributed into bag, namely completing can the preparation of teabag of prevention and therapy prostatosis again.

Embodiment 3:

Can the teabag of prevention and therapy prostatosis, be made up of following bulk drug according to weight part number: flower of JINHUAKUI 50 parts, 30 parts, JINHUAKUIZI powder, taraxacum 30 parts, Longhairy Antenoron Herb 30 parts and Flower of Arabian Jasmine 10 parts.

Step one, getting flower of JINHUAKUI raw material, be placed in steam oven, leading to is the steam insulation 40min of 110 DEG C into temperature, then 120 DEG C it are warming up to, insulation 10min, transfers in the thermostat container that temperature is 25 DEG C, keeps 15min, take out, being placed in baking oven to be dried, the temperature in baking oven is 60-70 DEG C, and time of drying is 105min, obtain flower of JINHUAKUI bulk drug, for subsequent use;

Step 2, get seed of JINHUAKUI raw material, first carry out coarse reduction with pulverizer, then it is placed in pressure cooker, 8min is steamed when 1.5Mpa, take out while hot after having steamed and it is placed in sealed can interior sealing more than 3h, then it is placed in baking oven to be dried and be no more than 10% to moisture content, obtains JINHUAKUIZI powder, for subsequent use;

Step 3, get bulk drug according to the weight part number in above-mentioned formula, first tree mallow, JINHUAKUIZI powder, taraxacum and Longhairy Antenoron Herb mixing are placed in supper micron mill, it is crushed to 200 orders, then take out and it is placed in iron pot, slow fire frying 6min, frying adds Flower of Arabian Jasmine while hot after terminating, mix, carrying out being distributed into bag, namely completing can the preparation of teabag of prevention and therapy prostatosis again.

Applied research data

One, flower of JINHUAKUI is on the impact of mouse prostate model of hyperplasia

1 experiment material

1.1 laboratory animal

Kunming mouse, male, 20��23g, Shandong province Experimental Animal Center provides, conformity certification number: NO.37005400000003. Raising in Henan College Of Traditional Chinese Medicine Experimental Animal Center, feed is sterilization feed, and bedding and padding are sterilization bedding and padding, provide by Henan Province's Experimental Animal Center. Drinking-water is sterilized distilled water. Rearing conditions: temperature 20��25 DEG C, relative humidity 40%��60%, natural lighting, 10, every cage, conventional raising, changes bedding and padding every other day.

1.2 Experimental agents and reagent

Flower of JINHUAKUI, is provided by Ruzhou Yin Di Industrial Co., Ltd.; Hibiscus manihot L. total flavones, is provided by Henan College Of Traditional Chinese Medicine chemistry room, content 60%. Finasteride tablet finasteride capsule, Wuhan Ren Fu medicine company limited liability company produces, lot number 20140202; Longbishu Jiaonang., Shijiazhuang Ke Di pharmaceutcal corporation, Ltd produces, lot number 150714; Testosterone propionate injection liquid, Shanghai GM medicine company limited-liability company produces, lot number 140902. Benzylpenicillin sodium for injection (4,000,000 unit), North China pharmacy company limited produces, lot number F4013504; Sodium chloride injection, Cologne, Henan pharmaceutcal corporation, Ltd produces, lot number C114092201-2; Formaldehyde, the Chemical Co., Ltd.'s production in pairs of China's Laiyang City, lot number 20140519; Chloral Hydrate, Tianjin recovery fine chemistry industry institute produces, lot number 20150606; Standone (DHT) test kit, R&D company of the U.S. produces, lot number 20150401A; Estradiol (E₂) test kit, R&D company of the U.S. produces, lot number 20150401A; Testis ketone (T) test kit, R&D company of the U.S. produces, lot number 20150401A.

1.3 laboratory apparatus

FA (N)/JA (N) series electronic balance, Shanghai Min Qiao precision instrument company limited; HWS12 type electric-heated thermostatic water bath, Shanghai Yiheng Scientific Instruments Co., Ltd; KDC-160HR high speed freezing centrifuge, Zhong Jia branch office of Keda Innovation Co., Ltd; OLYMPUSBX61 electric microscope, OLYMPUS company of Japan; 680 type microplate reader, BIO-RAD company of the U.S.; Adjustable pipette, Shanghai Lei Bo Analytical Instrument Co., Ltd.

2 experimental techniques

Get SPF level male KM mouse, body weight 20��23g, get 10 at random as blank group, make mouse prostate model of hyperplasia for another 9 groups, often organize 10, be respectively large, medium and small dosage Hibiscus manihot L. water decocting liquid group, large, medium and small dosage Hibiscus manihot L. total flavones group, LONGBISHU group, finasteride group and model group. the equal subcutaneous injection every day testosterone propionate 5mg/kg(of modeling mouse 9 groups is dissolved in soybean oil), continuous 3 weeks, blank group injection equivalent solvent. the group such as LONGBISHU group and finasteride gavages relative medicine respectively, and model group and blank group gavage same volume physiological saline. every day gastric infusion 1 time, successive administration 3 weeks. greatly, in, low dose of Hibiscus manihot L. total flavones group (72mg/kg, 36mg/kg, 18mg/kg is made into 7.2 with 0.5%CMC solution before use, 3.6, the Hibiscus manihot L. total flavones of 1.8mg/ml concentration is big, in, little suspension, administration volume 0.1ml/10g), greatly, in, low dose of Hibiscus manihot L. water decocting liquid group (3g/kg, 1.5g/kg, 0.75g/kg, being mixed with concentration respectively with physiological saline is 300, 150, 75mg/ml concentration big, in, little water decocting liquid Hibiscus manihot L., administration volume 0.1ml/10g), finasteride group (1.25mg/Kg, be equivalent to 15 times of clinical dosage, the Longbishu Jiaonang. suspension of 0.125mg/ml concentration it is made into before use with 0.5%CMC solution, administration volume 0.1ml/10g), LONGBISHU group (450mg/Kg, be equivalent to 15 times of clinical dosage, the Longbishu Jiaonang. suspension of 45mg/ml concentration it is made into before use with 0.5%CMC solution, administration volume 0.1ml/10g), model group (gavages same volume 0.5%CMC solution). blank group (gavaging same volume 0.5%CMC solution). every day gastric infusion 1 time, successive administration 3 weeks. after last administration, water 12h is can't help in 2h(fasting), after mouse weights, eye socket gets blood, centrifugal, separation of serum, illustrates according to test kit and surveys testis ketone (T), Standone (DHT), estradiol (E in serum₂) level; Then de-cervical vertebra puts to death mouse, gets rapidly prostate gland, epididymis, kidney, thymus gland and spleen, claims prostate gland weight in wet base, calculates prostate gland index (prostate gland index=prostate gland weight in wet base mg/ mouse body weight g); Prostate gland, epididymis, kidney, thymus gland and spleen being fixed in 10% formaldehyde solution, paraffin embedding, section, HE dyes, and light Microscopic observation respectively organizes the metamorphosis of prostata tissue.

3 statistical procedures methods

The medical statistics bag of data analysis SPSS17.0 carries out the statistical procedures of data data, measurement data mean number �� standard deviation (�� s) represent, comparing employing one-way analysis of variance between each group, the neat person of variance test uses LSD method, and heterogeneity of variance person adopts Ridit inspection with Games-Howell method inspection, ranked data.

4 experimental results

4.1 on the impact of prostatic hyperplasia model mouse prostate index, blood serum E2, T and DHT level

Table 1 Hibiscus manihot L. on the impact of prostatic hyperplasia model mouse prostate index, blood serum E2, T and DHT level (�� s, n=10)

* P < 0.01, * P < 0.05, represents compared with model group

Compared with blank group, model group prostate gland exponential sum serum T, DHT level significantly raises (P < 0.01), blood serum E2 level significantly reduces (P < 0.01), compared with model group, LONGBISHU group, finasteride group and big, in, low dose of Hibiscus manihot L. total flavones group, greatly, middle dosage Hibiscus manihot L. water decocting liquid group all can significantly reduce animal prostate gland exponential sum and significantly reduce serum DHT and T level (P < 0.01), remarkable increasing serum E2 level (P < 0.05), low dose of Hibiscus manihot L. total flavones group can significantly reduce animal prostate gland exponential sum serum DHT level (P < 0.01), obviously reduce serum T level (P < 0.05), remarkable increasing serum E2 level (P < 0.01).

4.2 on the impact of prostatic hyperplasia model mouse prostate tissue morphology

Each group mouse prostate histopathology is observed: blank group mouse prostate gland body, glandular epithelium and interstitial have no hyperplasia, substantially normally, see Fig. 1-1. Model group mouse prostate gland body and part glandular epithelium be obvious hyperplasia as seen, and the remarkable hyperplasia of interstitial, is shown in Fig. 1-2. Finasteride group mouse prostate glandular hyperplasia is obviously suppressed, just part glandular epithelium still hyperplasia, and interstitial is substantially normal, sees Fig. 1-3. LONGBISHU group mouse prostate glandular hyperplasia is obviously suppressed, just part glandular epithelium still hyperplasia, and interstitial is substantially normal, sees Fig. 1-4. Heavy dose of Hibiscus manihot L. total flavones group mouse prostate glandular hyperplasia, glandular epithelium hyperplasia are necessarily suppressed, and interstitial is substantially normal, sees Fig. 1-5. Middle dosage Hibiscus manihot L. total flavones group mouse prostate glandular hyperplasia, glandular epithelium hyperplasia are necessarily suppressed, and interstitial is substantially normal, sees Fig. 1-6. Low dose of Hibiscus manihot L. total flavones group mouse prostate gland body to some extent hyperplasia, interstitial slightly hyperplasia, see Fig. 1-7. Heavy dose of Hibiscus manihot L. water decocting liquid group mouse prostate glandular hyperplasia, glandular epithelium hyperplasia are necessarily suppressed, and interstitial is substantially normal, sees Fig. 1-8. Middle dosage Hibiscus manihot L. water decocting liquid group group mouse prostate glandular hyperplasia, glandular epithelium hyperplasia are necessarily suppressed, and interstitial is substantially normal, sees Fig. 1-9. Low dose of Hibiscus manihot L. total flavones group mouse prostate gland body hyperplasia to some extent, interstitial is hyperplasia slightly, sees Fig. 1-10.

According to each treated animal hyperplasia of prostate result of semi-quantitative standards determination experiment:

Table 2 Hibiscus manihot L. is on the impact of prostatic hyperplasia model mouse prostate tissue morphology

" _ " glandular epithelium and interstitial normal; "+" glandular epithelium hyperplasia, in column, accidental false nipple is formed or interstitial has a small amount of fibrillar connective tissue hyperplasia, proliferation of smooth muscle; " ++ " glandular epithelium hyperplasia, has a small amount of nipple or false nipple and interstitial to have a small amount of fibrillar connective tissue, proliferation of smooth muscle; " +++ " glandular epithelium hyperplasia, relatively polythelia or false papilla, in interstitial, fibrillar connective tissue, the obvious hyperplasia of unstriated muscle, become wide ribbon.

From upper table it can be seen that through Ridit inspection, with blank group ratio, significant hyperplasia of prostate pathology change (P 0.01) occurs in model group, the success of modeling type is described. With model group ratio, finasteride group, LONGBISHU group and heavy dose of Hibiscus manihot L. total flavones group can significantly alleviate model mice hyperplasia of prostate pathology change (P 0.01); In, low dose of Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group can obviously alleviate model hyperplasia of prostate pathology change (P 0.05).

4.3 on the impact of prostatic hyperplasia model mouse thymus tissue morphology

The pathological observation of each group mouse thymus tissue: blank group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is normally intensive, dense arrangement, sees Fig. 2-1. Model group thymus gland leaflet border is unclear, and cortex is thinning, and lymphocyte is normal but arranges sparse, sees Fig. 2-2. Finasteride group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-3. LONGBISHU group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-4. Heavy dose of Hibiscus manihot L. total flavones group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-5. Middle dosage Hibiscus manihot L. total flavones group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-6. Low dose of Hibiscus manihot L. total flavones group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-7. Heavy dose of Hibiscus manihot L. water decocting liquid group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-8. Middle dosage Hibiscus manihot L. water decocting liquid group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-9. Low dose of Hibiscus manihot L. water decocting liquid group thymus gland leaflet clear border, the boundary of medullary substance cortex is clear, and lymphocyte is intensive has no obvious pathological change, sees Fig. 2-10.

Employing micrometer, carries out mensuration the widest part to experiment each group of mouse thymus and the narrowest place tries to achieve all several, then be cortical thickness.

Table 3 Hibiscus manihot L. on the impact of the thickness of prostatic hyperplasia model mouse thymus cortex (��s)

Note: * * P < 0.01, * P < 0.05, represents compared with model group

Can find out from upper table, compared with blank group, model group thymic cortex thickness significantly reduces (P < 0.01), compared with model group, LONGBISHU group, finasteride group, large, medium and small dosage Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group all can remarkable elevation model mouse thymus cortical thickness (P < 0.01).

4.4 on the impact of prostatic hyperplasia model mouse spleen tissue morphology

The pathological observation result of each group mouse spleen tissue: the blank group red marrow of mouse spleen and white pulp boundary are clear, and splenic nodule is normal, and lymphocyte is normal, sees Fig. 3-1. Model group mice spleen brief summary diminishes, and lymphocyte is normal but sparse, sees Fig. 3-2. The red marrow of finasteride group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-3. The red marrow of LONGBISHU group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-4. Heavy dose of Hibiscus manihot L. total flavones red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-5. The middle dosage Hibiscus manihot L. total flavones red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-6. The low dose of Hibiscus manihot L. total flavones red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte are all substantially normal, see Fig. 3-7. Heavy dose of Hibiscus manihot L. water decocting liquid red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-8. The middle dosage Hibiscus manihot L. water decocting liquid red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte have no obvious pathological change, see Fig. 3-9. The low dose of Hibiscus manihot L. water decocting liquid red marrow of group mouse spleen and white pulp boundary are clear, and splenic nodule, lymphocyte are all substantially normal, see Fig. 3-10.

Adopting micrometer to be measured by splenic nodule, three splenic nodules are surveyed in the choosing of every animal spleen, and calculating both sides splenic nodule size centered by spleen arteriole, to try to achieve all numbers be splenic nodule thickness.

Table 4 Hibiscus manihot L. on the impact of the thickness of prostatic hyperplasia model mouse spleen cortex (��s)

Note: * * P < 0.01, * P < 0.05, represents compared with model group

Can find out from upper table, compared with blank group, model group splenic nodule thickness significantly reduces (P < 0.01), compared with model group, LONGBISHU group, finasteride group, large, medium and small dosage Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group all can significantly raise animal splenic nodule thickness (P < 0.01).

4.5 on the impact of prostatic hyperplasia model mouse kidney tissue form

The pathological observation result of each group mouse kidney tissue: blank group mouse kidney renal glomerulus, renal capsule, uriniferous tubules and epithelial cell are all normal, see Fig. 4-1. Model group mouse kidney renal glomerulus, renal capsule, uriniferous tubules have no obvious pathological change, see Fig. 4-2. Finasteride group mouse kidney renal glomerulus, renal capsule, uriniferous tubules have no obvious pathological change, see Fig. 4-3. LONGBISHU group mouse kidney renal glomerulus, renal capsule, uriniferous tubules have no obvious pathological change, see Fig. 4-4. Heavy dose of Hibiscus manihot L. total flavones group mouse kidney renal glomerulus, renal capsule and uriniferous tubules, there are no obvious pathological change, are shown in Fig. 4-5. Middle dosage Hibiscus manihot L. total flavones group mouse kidney renal glomerulus, renal capsule and uriniferous tubules, there are no obvious pathological change, are shown in Fig. 4-6. Low dose of Hibiscus manihot L. total flavones group mouse kidney renal glomerulus, renal capsule and uriniferous tubules and epithelial cell are all substantially normal, see Fig. 4-7. Heavy dose of Hibiscus manihot L. total flavones group mouse kidney renal glomerulus, renal capsule and uriniferous tubules, there are no obvious pathological change, are shown in Fig. 4-8. Middle dosage Hibiscus manihot L. water decocting liquid group mouse kidney renal glomerulus, renal capsule and uriniferous tubules, there are no obvious pathological change, are shown in Fig. 4-9. Low dose of Hibiscus manihot L. water decocting liquid group mouse kidney renal glomerulus, renal capsule and uriniferous tubules and epithelial cell are all substantially normal, see Fig. 4-10.

4.6 on the impact of prostatic hyperplasia model mouse testis tissue morphology

The pathological observation result of each group mouse testis tissue: in blank group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-1; In model group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-2; In finasteride group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are substantially normal, and sperm is normal, sees Fig. 5-3; In LONGBISHU group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are substantially normal, and sperm is normal, sees Fig. 5-4; In Hibiscus manihot L. total flavones heavy dose group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-5; In Hibiscus manihot L. total flavones, in dosage group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, see according to Fig. 5-6; In Hibiscus manihot L. total flavones small dose group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-7; In Hibiscus manihot L. water decocting liquid heavy dose group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-8; In Hibiscus manihot L. water decocting liquid, in dosage group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-9; In Hibiscus manihot L. water decocting liquid small dose group mouse testis, the spermatogoniums at different levels in convoluted seminiferous tubule are normal, and sperm is normal, sees Fig. 5-10;

4.7 on the impact of prostatic hyperplasia model mouse epididymis tissue morphology

The pathological observation result of each group mouse epididymis tissue: in blank group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-1 in pipe chamber; In model group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-2 in pipe chamber; In finasteride group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-3 in pipe chamber; In LONGBISHU group mouse epididymis, tubulose epithelial cell base is substantially normal, has a large amount of sperms, see Fig. 6-4 in pipe chamber; In Hibiscus manihot L. total flavones heavy dose group mouse epididymis, tubulose epithelial cell is substantially normal, and pipe has sperm in chamber, sees Fig. 6-5; In Hibiscus manihot L. total flavones, in dosage group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-6 in pipe chamber; In Hibiscus manihot L. total flavones small dose group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-7 in pipe chamber; In Hibiscus manihot L. water decocting liquid heavy dose group mouse epididymis, tubulose epithelial cell is substantially normal, and pipe has sperm in chamber, sees Fig. 6-8; In Hibiscus manihot L. water decocting liquid, in dosage group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-9 in pipe chamber; In Hibiscus manihot L. water decocting liquid small dose group mouse epididymis, tubulose epithelial cell is substantially normal, has a large amount of sperms, see Fig. 6-10 in pipe chamber.

Brief summary

Adopting testosterone propionate subcutaneous injection to make the success of mouse prostate model of hyperplasia, model group prostate gland exponential sum serum T, DHT level significantly raise, and blood serum E2 level significantly reduces; There is remarkable hyperplasia in prostata tissue; There is remarkable atrophy in thymic cortex and spleen cortex.

Hibiscus manihot L. water decocting liquid group and each dosage Hibiscus manihot L. total flavones group all can reduce animal hyperplasia of prostate index, reduce serum DHT, T level, raise E2 level, alleviate the related pathologies change of prostata tissue. Large, medium and small dosage Hibiscus manihot L. total flavones group and big or middle dosage Hibiscus manihot L. water decocting liquid group all can significantly reduce animal prostate gland exponential sum and significantly reduce serum DHT and T level, remarkable increasing serum E2 level, low dose of Hibiscus manihot L. total flavones group can significantly reduce animal prostate gland exponential sum serum DHT level, obviously reduce serum T level, remarkable increasing serum E2 level; Heavy dose of Hibiscus manihot L. total flavones group can significantly alleviate the change of model mice hyperplasia of prostate pathology, in, low dose of Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group can obviously alleviate model hyperplasia of prostate and epididymis pathological change; Large, medium and small dosage Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group all can remarkable elevation model mouse thymus cortical thicknesses; Large, medium and small dosage Hibiscus manihot L. total flavones group and large, medium and small dosage Hibiscus manihot L. water decocting liquid group all can significantly raise animal splenic nodule thickness. Prompting Hibiscus manihot L. and Hibiscus manihot L. total flavones all can significantly improve mouse prostate hyperplasia pathological change.

Two, flower of JINHUAKUI is on the impact of the scorching model of mouse prostate

1 experiment material

1.1 laboratory animal

Mouse, Kunming kind, male, 25��30g, animal conformity certification numbering 0021890, is purchased from Shandong province medical experiment animal center, raises at Henan College Of Traditional Chinese Medicine Experimental Animal Center (credit number SYXK(Henan) 2015-0005); Feed is sterilization feed, and bedding and padding are sterilization bedding and padding, provide by Henan Province's Experimental Animal Center; Drinking-water is sterilized distilled water; Rearing conditions: temperature 20��25 DEG C, relative humidity 40%��60%, natural lighting, 10, every cage, conventional raising, changes bedding and padding every other day.

1.2 Experimental agents and reagent

Flower of JINHUAKUI, has tafelberg Yin Di group to provide. QIANLIEKANG PIAN (every sheet is containing rape petal pollen 0.5g), Kang Enbei Zhejiang Pharmaceutical Co, lot number 140522; XIAOZHILING ZHUSHEYE (often props up 10ml), Jian, Jilin Province Yi Sheng medicine company limited-liability company, lot number 13102402; Chloral Hydrate, Tianjin recovery fine chemistry industry institute, lot number 20130606; Benzylpenicillin sodium for injection, Huabei Pharmaceutic Co., Ltd, lot number F4013504; Sodium chloride injection, Cologne, Henan pharmaceutcal corporation, Ltd, lot number C114092201-2; Formaldehyde, Laiyang City Chemical Co., Ltd. in pairs of China, lot number 20140519.

1.3 laboratory apparatus

JY601 electronic scales, Shanghai Min Qiao Medical Devices Co., Ltd.; FA(N)/JA(N) series electronic balance, Shanghai Min Qiao precision instrument company limited; Adjustable pipette, Shanghai Lei Bo Analytical Instrument Co., Ltd; OLYMPUS opticmicroscope; The medical image analysis system of BL-2000, Chengdu Tai Meng Science and Technology Ltd..

2 experimental techniques

2.1 modelings and grouping

Laboratory routine was raised after 1 week, selected the male mice of body weight at 28��30g for experiment. Choosing 10 at random as blank group, all the other are for making prostatitis model. Modeling mouse is weighed respectively, and abdominal injection 10% Chloral Hydrate (0.03ml/10g), anaesthetizes after successfully, uses alcohol disinfecting skin, performs the operation; Reach abdominal cavity in lower abdomen median incision, it is proposed to bladder and both sides seminal vesicle, expose bladder dorsal part prostate gland (dorsal part leaf), accurately inject 25% XIAOZHILING ZHUSHEYE 0.02ml with microsyringe, send abdominal cavity back to; Layering suture muscles, skin, use alcohol disinfecting wound, puts back to mouse cage routine and raise after mouse is clear-headed; Intramuscularly penicillin 200,000 u/kg after art, is used in conjunction 3 days, with preventing infection. After art the 8th day, select the good modeling mouse of recovery situation 50, it is divided into 5 groups at random by body weight, often organize 10, i.e. model group, QIANLIEKANG group, high, medium and low dosage flower of JINHUAKUI group.

2.2 medication

2.2.1 the preparation of experimental drug

The high, medium and low dosage group mouse of flower of JINHUAKUI daily dosage be respectively 3g/kg, 1.5g/kg, 0.75g/kg, with physiological saline be mixed with respectively concentration be 150,75, the solution of 37.5mg/ml; QIANLIEKANG PIAN: QIANLIEKANG is by 15 times of administrations of the clinical consumption of adult. The every per daily dose of mouse is 1.5g/kg, is made into, with physiological saline, the suspension that concentration is 0.075g/ml before use; XIAOZHILING ZHUSHEYE: be mixed with the concentration of 25% before use with sterilized water for injection.

2.2.2 dosage and method

Blank group and model group, from modeling the 8th day, give distilled water gavage by 0.2ml/10g body weight, every day 1 time, continuous 3 weeks. Positive controls, from modeling the 8th day, gives QIANLIEKANG JIAONANG suspension oral gavage by 0.2ml/10g body weight, every day 1 time, continuous 3 weeks. Flower of JINHUAKUI high, medium and low dosage group gives corresponding medicine gavage by 0.2ml/10g body weight.

2.3 index determining

Amount of drinking water in mouse 24h: the 14th day and the 28th day after modeling measures respectively often organizes the amount of drinking water in mouse 24h.

After last administration 1h (fasting 12 hours), weigh, put to death mouse afterwards, take out rapidly prostata tissue, get about 10mg prostata tissue, shred, add a 20 times distilled water, abundant jolting, (the full visual field is 4 points to carry out white blood cell count(WBC) and lecithin density scores, 3/4 visual field is 3 points, and 1/2 visual field is 2 points, and 1/4 visual field is 1 point). Separately get part prostata tissue and testis, epididymis, thymus gland, spleen be fixed in 10% formalin solution, paraffin embedding, section, HE dyes, and light Microscopic observation respectively organizes the techtology change of prostate gland, testis, epididymis, thymus gland, spleen.

2.4 statistical procedures methods

Data analysis SPSS13.0forwindows statistical package carries out the statistical procedures of data data, measurement data mean number �� standard deviation (�� s) represent, comparing with one-way analysis of variance between group, enumeration data adopts Ridit to analyze.

3 experimental results

3.1 on the impact of prostatitis model mice amount of drinking water

After modeling the 14th day and the 28th day respectively, measures respectively and often organizes the amount of drinking water of mouse in 24h, the results are shown in Table 5.

Table 5 flower of JINHUAKUI is on the impact (ml) of prostatitis model mice amount of drinking water

From upper table it can be seen that model group mouse after modeling the 14th day and the 28th day amount of drinking water all few than blank group, reduce 6.52% and 17.02% respectively, point out along with the prolongation of modeling time, model mice prostatitis symptom increases the weight of. With model group ratio, high, medium and low dosage flower of JINHUAKUI group can make model mice amount of drinking water increase by 3.48%, 13.48% respectively the 14th day and the 28th day, and 2.17%, 12.29%, 1.96%, 7.57%, illustrate along with administration time extends, prostatitis symptom is improved more obvious.

3.2 on the impact of leukocyte count and lecithin density in prostatitis model mice prostata tissue

By corresponding experimental technique and standard, observe and record total white blood cells and lecithin density integral in prostata tissue, the results are shown in Table 6.

Table 6 flower of JINHUAKUI on the impact of leukocyte count and lecithin density in prostatitis model mice prostata tissue (�� s)

�� represents compared with model group, P < 0.01

From upper table it can be seen that with blank group ratio, in model group prostata tissue leukocyte count significantly increase (P 0.01), lecithin density integral significantly reduce (P 0.01), illustrate make prostatitis model success. Compared with model group, in high, medium and low dosage flower of JINHUAKUI group and QIANLIEKANG group prostate gland, leukocyte count all significantly reduces (P 0.01), prostate gland lecithin density integral all significantly increases (P 0.01), and obvious with flower of JINHUAKUI height, middle dose effect. Flower of JINHUAKUI has the effect improving prostatitis model mice prostatitis symptom.

3.3 on the impact of prostatitis model mice prostate gland, testis, epididymis, kidney, Thymus and spleen tissue morphology

3.3.1 on the impact (such as Fig. 7) of mouse prostate tissue morphology

Each group prostatic histomorphology is observed: blank group prostate gland is normal, and all corrugated arrangement of glandular epithelium, interstitial has no the hyperplasia of cell infiltration and fiber. There is obvious hyperplasia in model group prostate gland, and gland chamber is expanded, and has a large amount of inflammatory cells to ooze out, mainly contain neutrophil leucocyte and lymphocyte in gland chamber, and interstitial also has the hyperplasia of obvious cell infiltration and fiber. QIANLIEKANG group prostate gland part expands, and a small amount of fibroplasia and a small amount of inflammatory cell, occurs in the corrugated arrangement of part glandular epithelium around gland body. High dosage Hibiscus manihot L. group prostate gland is normal, and the corrugated arrangement of glandular epithelium, interstitial has no the hyperplasia of fiber, accidental cell infiltration.

Middle dosage Hibiscus manihot L. group prostate gland gland chamber recovers normal substantially, and the gland epithelial cells corrugated arrangement of major part, has a small amount of fibroplasia and inflammatory cell in interstitial. There is obvious hyperplasia in low dosage Hibiscus manihot L. group prostate gland, and gland chamber is obviously expanded, and epithelium becomes flat in flats, has a small amount of fibroplasia and inflammatory cell infiltration in interstitial.

Table 7 Hibiscus manihot L. is on the impact of prostatitis model mice prostata tissue

"-" prostate gland, glandular epithelium and interstitial all normal; "+" the rare expansion of prostate gland, rare fibroplasia and a small amount of cell infiltration around gland body; The obvious hyperplasia of " ++ " prostate gland, the expansion of gland chamber, have the glandular epithelium come off on a small quantity and inflammatory cell in gland chamber, interstitial has a small amount of fibroplasia and cell infiltration; The obvious hyperplasia of " +++ " prostate gland, the expansion of gland chamber, have the glandular epithelium come off on a small quantity and a large amount of inflammatory cells in gland chamber, interstitial has obvious fibroplasia and cell infiltration.

From upper table it can be seen that through Ridit inspection, with blank group ratio, significant prostatitis pathological change (P 0.01) occurs in model group, the success of modeling type is described. With model group ratio, dosage Hibiscus manihot L. group high, middle can significantly alleviate model prostatitis pathological change (P 0.01), QIANLIEKANG group and low dosage Hibiscus manihot L. group can obviously alleviate model prostatitis pathological change (P 0.05), taking high dosage Hibiscus manihot L. to the improvement result of mouse prostate inflammation as excellent.

3.3.2 on the impact (such as Fig. 8) of mouse kidney tissue form

Each group renal tissue morphologic observation: blank group renal glomerulus, renal capsule, uriniferous tubules and epithelial cell are all normal. Model group renal glomerulus, renal capsule, uriniferous tubules have no obvious pathological change. QIANLIEKANG group renal glomerulus is substantially normal, and renal capsule no abnormality seen, vacuolar degeneration occurs in minority renal cells. High dosage Hibiscus manihot L. group renal glomerulus, renal capsule are normal; There is vacuolar degeneration in minority renal cells. Middle dosage Hibiscus manihot L. group renal glomerulus, renal capsule and uriniferous tubules are there are no obvious pathological change. Low dosage Hibiscus manihot L. group renal glomerulus, renal capsule and uriniferous tubules and epithelial cell are all substantially normal.

Table 8 Hibiscus manihot L. is on the impact of prostatitis model mice renal tissue

" " renal glomerulus, renal capsule, uriniferous tubules and epithelial cell are all normal; "+" renal glomerulus, renal capsule, uriniferous tubules and epithelial cell about have 25% cell to occur bubble becomes; " ++ " renal glomerulus, renal capsule, uriniferous tubules and epithelial cell about have 50% cell to occur, and bubble becomes; " +++ " renal glomerulus, renal capsule, uriniferous tubules and epithelial cell about have 75% cell to occur, and bubble becomes.

Can find out from upper table, blank group renal tissue is all normal, with blank group ratio, model group has 2 rat kidney slight pathological change occur, each administration group all can make kidney pathological change reduce, QIANLIEKANG combination high dosage Hibiscus manihot L. group respectively has 1 rat to have slight Renal pathology, in, low dose group Hibiscus manihot L. group is without Renal pathology.

3.3.3 on the impact (such as Fig. 9) of mouse testis tissue morphology

Under light microscopic mouse testis structure observation is visible: the high, medium and low each group of testis convoluted seminiferous tubule spermatogonium at different levels of blank group, pathological model group, Hibiscus manihot L., sustenticular cell and interstitial are all normal

3.3.4 on the impact of mouse epididymis tissue morphology

Each group mouse epididymis tissue morphology: epididymal duct and the interstitial of blank group epididymis are all normal. Containing abundant sperm in epididymal duct in model group epididymis, obvious fibroplasia and cell infiltration occur around pipe chamber. Containing abundant sperm in QIANLIEKANG group epididymal duct, around pipe chamber, there are a large amount of fibroplasia and cell infiltration. High dosage Hibiscus manihot L. group epididymal duct and mesenchymal cell are all normal. The middle dosage Hibiscus manihot L. group obvious thinning minimizing of epididymis surrounding annulus hyperplasia, inflammatory cell obviously reduces. Visible obviously fibroplasia and minority inflammatory cell around low dosage Hibiscus manihot L. group epididymis.

Table 9 Hibiscus manihot L. is on the impact of prostatitis model mice epididymis tissue

Without fibroplasia and cell infiltration around "-" epididymis gland body, all normally; Minority fibroplasia and cell infiltration is had around "+" epididymis gland body; Obvious fibroplasia and cell infiltration is had around " ++ " epididymis gland body; A large amount of fibroplasia and cell infiltration is had around " +++ " epididymis gland body.

From upper table it can be seen that through Ridit inspection, with blank group ratio, significant epididymis pathological change (P 0.01) occurs in model group, the success of modeling type is described. With model group ratio, dosage Hibiscus manihot L. group high, middle can significantly alleviate model epididymis pathological change (P 0.01), QIANLIEKANG group can obviously alleviate model epididymis pathological change (P 0.05), and low dosage Hibiscus manihot L. group only has the trend (P < 0.05) alleviating epididymis pathological change.

3.3.5 on the impact (such as Figure 10) of Thymus and spleen tissue

Under light microscopic, the visible Thymus and spleen tissue morphology to each group of mouse is all without obvious ANOMALOUS VARIATIONS.

4. brief summary

The scorching model success in XIAOZHILING ZHUSHEYE induced mice prostatitis, can make mouse amount of drinking water reduce, and Cervical mucus, lecithin decrease in density in prostata tissue, remarkable pathological change occur in prostate gland, epididymis. The improvement result that the scorching model in XIAOZHILING ZHUSHEYE induced mice prostatitis has been had by Hibiscus manihot L., model mice amount of drinking water can be increased, can significantly reduce leukocyte count in prostata tissue, significantly increase lecithin density, significantly alleviate the pathological change of prostate gland, epididymis tissue.

Claims

1. one kind can the teabag of prevention and therapy prostatosis, it is characterised in that: be made up of following bulk drug according to weight part number: flower of JINHUAKUI 40-60 part, JINHUAKUIZI powder 20-40 part, taraxacum 20-40 part, Longhairy Antenoron Herb 20-40 part and Flower of Arabian Jasmine 5-15 part.

2. a kind of as claimed in claim 1 can the preparation method of teabag of prevention and therapy prostatosis, it is characterised in that: comprise the following steps:

3. a kind of as claimed in claim 2 can the preparation method of teabag of prevention and therapy prostatosis, it is characterised in that: the flower of JINHUAKUI starting material described in step one before being placed in steam oven first with the warm water soaking 20-60min that temperature is 40-50 DEG C.

4. a kind of as claimed in claim 2 can the preparation method of teabag of prevention and therapy prostatosis, it is characterized in that: described in step 3, the concocting method of Longhairy Antenoron Herb bulk drug is: get Longhairy Antenoron Herb raw material, it is placed in steam oven, leading to is the steam insulation 45min of 110 DEG C into temperature, then 120 DEG C it are warming up to, lead to and make air pressure inside reach 1.5MPa into nitrogen, Longhairy Antenoron Herb starting material in abrupt release steam oven after lasting 5min, transfer in the thermostat container that temperature is 30 DEG C, insulation 30min, take out, it is placed in baking oven to be dried, it is no more than 10% to moisture content, namely Longhairy Antenoron Herb bulk drug is obtained.