CN105613721A - Preservative and preparation method and application thereof - Google Patents
Preservative and preparation method and application thereof Download PDFInfo
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- CN105613721A CN105613721A CN201511016828.7A CN201511016828A CN105613721A CN 105613721 A CN105613721 A CN 105613721A CN 201511016828 A CN201511016828 A CN 201511016828A CN 105613721 A CN105613721 A CN 105613721A
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- preservation agent
- synephrine
- hesperidine
- preservation
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000003755 preservative agent Substances 0.000 title abstract 4
- 230000002335 preservative effect Effects 0.000 title abstract 4
- YRCWQPVGYLYSOX-UHFFFAOYSA-N synephrine Chemical compound CNCC(O)C1=CC=C(O)C=C1 YRCWQPVGYLYSOX-UHFFFAOYSA-N 0.000 claims abstract description 52
- 238000004321 preservation Methods 0.000 claims abstract description 46
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000003756 stirring Methods 0.000 claims abstract description 31
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 claims abstract description 26
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 claims abstract description 26
- 229960003684 oxedrine Drugs 0.000 claims abstract description 26
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 19
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 19
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 18
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims abstract description 16
- DCXXMTOCNZCJGO-UHFFFAOYSA-N Glycerol trioctadecanoate Natural products CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 4
- 240000003768 Solanum lycopersicum Species 0.000 claims abstract description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 42
- 239000000284 extract Substances 0.000 claims description 30
- 235000013399 edible fruits Nutrition 0.000 claims description 20
- 238000000605 extraction Methods 0.000 claims description 20
- 244000044283 Toxicodendron succedaneum Species 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 6
- -1 purity is 90%��95% Substances 0.000 claims description 6
- 240000004307 Citrus medica Species 0.000 claims description 5
- 238000000265 homogenisation Methods 0.000 claims description 5
- 238000001953 recrystallisation Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 235000011430 Malus pumila Nutrition 0.000 claims description 4
- 235000015103 Malus silvestris Nutrition 0.000 claims description 4
- 235000014443 Pyrus communis Nutrition 0.000 claims description 3
- 240000001987 Pyrus communis Species 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 241000220225 Malus Species 0.000 claims 1
- 235000012055 fruits and vegetables Nutrition 0.000 abstract description 6
- 244000141359 Malus pumila Species 0.000 abstract description 4
- 239000001100 (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one Substances 0.000 abstract 2
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 abstract 2
- APSNPMVGBGZYAJ-GLOOOPAXSA-N clematine Natural products COc1cc(ccc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2 APSNPMVGBGZYAJ-GLOOOPAXSA-N 0.000 abstract 2
- 238000010438 heat treatment Methods 0.000 abstract 2
- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 abstract 2
- 229940025878 hesperidin Drugs 0.000 abstract 2
- 239000004922 lacquer Substances 0.000 abstract 2
- ARGKVCXINMKCAZ-UHFFFAOYSA-N neohesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(CO)O3)OC3C(C(O)C(O)C(C)O3)O)=CC(O)=C2C(=O)C1 ARGKVCXINMKCAZ-UHFFFAOYSA-N 0.000 abstract 2
- 241000220324 Pyrus Species 0.000 abstract 1
- 241000219094 Vitaceae Species 0.000 abstract 1
- 235000021016 apples Nutrition 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 235000021021 grapes Nutrition 0.000 abstract 1
- 235000021017 pears Nutrition 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 21
- 102000003992 Peroxidases Human genes 0.000 description 10
- 102000019197 Superoxide Dismutase Human genes 0.000 description 10
- 108010012715 Superoxide dismutase Proteins 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 description 10
- 229940032362 superoxide dismutase Drugs 0.000 description 10
- 102000016938 Catalase Human genes 0.000 description 9
- 108010053835 Catalase Proteins 0.000 description 9
- 102000030523 Catechol oxidase Human genes 0.000 description 9
- 108010031396 Catechol oxidase Proteins 0.000 description 9
- 108700023158 Phenylalanine ammonia-lyases Proteins 0.000 description 9
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 6
- 235000013311 vegetables Nutrition 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 150000003254 radicals Chemical class 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 238000004378 air conditioning Methods 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 230000007760 free radical scavenging Effects 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 239000005913 Maltodextrin Substances 0.000 description 2
- 229920002774 Maltodextrin Polymers 0.000 description 2
- 239000006087 Silane Coupling Agent Substances 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 229940035034 maltodextrin Drugs 0.000 description 2
- 238000005502 peroxidation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 2
- 229920001661 Chitosan Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000007259 addition reaction Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 229940075507 glyceryl monostearate Drugs 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000002960 lipid emulsion Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Storage Of Fruits Or Vegetables (AREA)
Abstract
The invention discloses a preservative, prepared from the following components: 10 to 50 g of lacquer wax, 15 to 25 g of Tefose2000 emulsifying agent, 50 to 100 g of hesperidin, 10 to 50 g of synephrine, 10 to 30 g of citric acid and 10 to 30 g of stearin. A preparation method for the preservation comprises the following steps of firstly adding one part of the lacquer wax into 30 to 50 parts of water, heating to 50 to 60 DEG C, stirring in a high-speed homogenizer at a stirring speed of 5000 to 10000 r/min for 5 to 10 min, then heating to 55 to 70 DEG C, and continuing to stir for 4 to 8 min and then preserving heat after the addition of the stearin; then mixing the hesperidin with the synephrine, stirring uniformly at a temperature of 60 to 70 DEG C, then dropwise adding mixed drops into the mixed solution obtained in the step (1), and stirring at a speed of 6000 to 10000 r/min for 10 to 15 min; finally adding the Tefose2000 emulsifying agent and the citric acid, stirring for 3 to 6 min, and cooling to room temperature so as to obtain the preservative. The preservative prepared according to the preservation method disclosed by the invention can be applied to the preservation of fruits and vegetables, such as lotus seedpods, apples, pears, tomatoes and grapes.
Description
Technical field
The invention belongs to technical field of food additives, it is specifically related to a kind of preservation agent and its preparation method, purposes.
Background technology
Along with growth in the living standard, the demand of the fruit that different areas Various Seasonal is produced is strengthened by people gradually, but, fruit vegetables transport and storage process in easily occur variable color, rot etc. problem, not only affect fruit-vegetable quality, also create huge financial loss. At present, the product for preserving fruit and vegetable utilizing is more, but there is release period uneven phenomenon short, fresh-keeping, in addition, there is the raw material that human body cannot digest in formula, endangers healthy.
Patent CN104082402A discloses a kind of fruit and vegetable fresh-keeping agent, and further disclose constituent and mass percent, namely by lacquer tree fat emulsion 20-30%, lacquer tree seed nucleolus oil 0.5-2%, water 45-70%, ethanol 5-15%, nano titanium oxide 0.05-0.1%, propylene glycol or glycerol 0.5-3%, tween 0.5-2%, silane coupling agent 0.01-0.02%, chitosan 1-2%, acetic acid 0.1-0.5%, maltodextrin 0.5-2%, sucrose fatty ester 0.1-1%, glyceryl monostearate 0.5-2%, although this formula has been applied to lacquer tree fat, lacquer tree seed nucleolus oil, the natural matters such as maltodextrin, but also it is applied to nano titanium oxide, the composition that the human bodies such as silane coupling agent are nondigestible, in addition, its addO-on therapy is too much, apply it on preserving fruit and vegetable utilizing, pericarp generally can not eat, but as the pericarp of the fruit such as apple contains some benefit materials, pericarp is cut to be removed, benefit materials wherein also can be wasted thereupon.
Summary of the invention
Goal of the invention: the object of the invention is for the deficiencies in the prior art, it is provided that the preservation agent of a kind of good refreshing effect, pure natural and its preparation method, purposes.
Technical scheme: a kind of preservation agent of the present invention, comprises following component, lacquer tree fat 10��50g, Tefose2000 emulsifying agent 15��25g, hesperidine 50��100g, synephrine 10��50g, citric acid 10��30g, stearin 10��30g.
Preferably, described preservation agent comprises following component: lacquer tree fat 30g, Tefose2000 emulsifying agent 20g, hesperidine 85g, synephrine 36g, citric acid 16g, stearin 23g.
Preferably, for obtaining the hesperidine of this formula applicable, described hesperidine is Pericarpium Citri junoris extract, purity is 90%��95%, concrete extracting method is homogenate extraction method: be that 65%��70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35��45 by orange peel dried powder and mass concentration, with flash extracter with 1.8 �� 105R/min��2.0 �� 105VELOCITY EXTRACTION 4��the 10min of r/min, then through centrifugal, filters, and concentrated, recrystallization, obtains the Pericarpium Citri junoris extract that hesperidine purity is 90%��95%.
Preferably, for obtaining the synephrine of this formula applicable, described synephrine is Furctus Aurantii Immaturus extract, purity is 55%��65%, concrete extracting method is homogenate extraction method: be that 60%��70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35��45 by dried immature fruit of citron orange dry fruit powder and mass concentration, with flash extracter with 1.8 �� 105R/min��2.0 �� 105VELOCITY EXTRACTION 4��the 10min of r/min, then through centrifugal, filters, and concentrated, purifying, obtains the Furctus Aurantii Immaturus extract that synephrine purity is 55%��65%.
The preparation method of above-mentioned preservation agent, comprises the following steps:
(1) 1 part of lacquer tree fat is added in 30��50 parts of water, being heated to 50��60 DEG C, stir evenly in high-speed homogenization machine, stirring velocity is 5000��10000r/min, it is warming up to 55��70 DEG C after churning time 5��10min, adds after stearin continues stirring 4��8min and be incubated;
(2) mix containing the Pericarpium Citri junoris extract of hesperidine and the Furctus Aurantii Immaturus extract containing synephrine, and stir evenly at 60��70 DEG C, then mixed solution is added dropwise in the mixing liquid in step (1), 10��15min is stirred under 6000��10000r/min, it is cooled to room temperature after finally adding Tefose2000 emulsifying agent and citric acid stirring 3��6min, obtains preservation agent.
Preferably, the speed heated up described in step (1) is 2��5 DEG C/min.
Preferably, for obtaining homogeneous preservation agent, described in step (2), the rate of addition of mixed solution is 3��6ml/min.
Preservation agent prepared by the present invention can be used for the fresh-keeping of the seedpod of the lotus, apple, pear, tomato and grape.
Useful effect: the present invention adopts the natural plant extracts such as lacquer tree fat, hesperidine, synephrine to prepare the fruit and vegetable fresh-keeping agent of natural nuisance-free; one layer of fine and close protection film can be formed at fruit surface; prevent fruit vegetables epidermis generation brown stain on the one hand; reduce water loss on the other hand; thus maintain fruit-vegetable quality, the storage period of extending fruit.
Accompanying drawing explanation
Fig. 1 is respiratory intensity broken line graph in embodiment 1;
Fig. 2 is brown stain degree broken line graph in embodiment 1;
Fig. 3 is total phenol content broken line graph in embodiment 1;
Fig. 4 is mda content broken line graph;
Fig. 5 is DPPH free radical scavenging activity broken line graph;
Fig. 6 is ultra-oxygen anion free radical (O2-) clearance rate broken line graph;
Fig. 7 is the active broken line graph of phenylalanine ammonia lyase (PAL);
Fig. 8 is the active broken line graph of polyphenoloxidase (PPO);
Fig. 9 is the active broken line graph of catalase (CAT);
Figure 10 is the active broken line graph of peroxidase (POD);
Figure 11 is the active broken line graph of superoxide-dismutase (SOD).
Embodiment
Technical solution of the present invention being described in detail below by accompanying drawing, but protection scope of the present invention is not limited to described embodiment, in embodiment, method is ordinary method if no special instructions, it may also be useful to reagent be conventional commercially available if no special instructions
Embodiment 1: a kind of preservation agent, comprises following component: lacquer tree fat 30g, Tefose2000 emulsifying agent 20g, hesperidine 85g, synephrine 36g, citric acid 16g, stearin 23g. Described hesperidine is Pericarpium Citri junoris extract, and concrete extracting method is homogenate extraction method: be that 70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:40 by orange peel dried powder and mass concentration, with flash extracter with 1.9 �� 105The VELOCITY EXTRACTION 8min of r/min, then through centrifugal, filters, and concentrated, recrystallization, obtains the Pericarpium Citri junoris extract that hesperidine purity is 95%; Described synephrine is Furctus Aurantii Immaturus extract, and concrete extracting method is homogenate extraction method: be that 65% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:40 by dried immature fruit of citron orange dry fruit powder and mass concentration, with flash extracter with 1.9 �� 105The VELOCITY EXTRACTION 8min of r/min, then through centrifugal, filters, and concentrated, purifying, obtains the Furctus Aurantii Immaturus extract that synephrine purity is 65%.
The preparation method of above-mentioned preservation agent, comprises the following steps:
(1) lacquer tree fat is added in 1000g water, being heated to 55 DEG C, stir evenly in high-speed homogenization machine, stirring velocity is 8000r/min, it is warming up to 65 DEG C by the heat-up rate of 2��5 DEG C/min after churning time 8min, adds after stearin continues to stir 6min and be incubated;
(2) mix containing the Pericarpium Citri junoris extract of hesperidine and the Furctus Aurantii Immaturus extract containing synephrine, and stir evenly at 65 DEG C, then by the rate of addition of 3��6ml/min, mixed solution is added dropwise in the mixing liquid in step (1), 12min is stirred under 8000r/min, it is cooled to room temperature after finally adding Tefose2000 emulsifying agent and citric acid stirring 4min, obtains preservation agent.
The preservation agent prepared in the present embodiment is used for the fresh-keeping of the seedpod of the lotus, and its fresh-keeping effect of tracking and testing, concrete operation is as follows: is immersed by the seedpod of the lotus and drains after taking out after 5s in the preservation agent of above-mentioned preparation, then it is positioned over uncovered storage in air-conditioning preservation box at normal temperatures, within every two days in storage, measures each fresh-keeping index; For the fresh-keeping effect of preservation agent in checking the present embodiment, controlled trial and blank group and clear water control group are also set; Described blank group is the seedpod of the lotus being directly placed on uncovered storage in air-conditioning preservation box without any process; Described clear water control group drains for soaking to take out after 5s through clear water, is then positioned over the seedpod of the lotus of uncovered storage in air-conditioning preservation box at normal temperatures; Seedpod of the lotus freshness before control treatment used in three groups is consistent.
Fig. 1 is the respiratory intensity broken line graph of test group and control group in the present embodiment, passing in time as can be seen from Figure, the respiratory intensity of test group is slightly better than control group on the 2nd day in test, all it is weaker than control group afterwards, thus prove, can effectively reduce the respiration of fruit and vegetable surfaces after adopting preservation agent, ensure the fresh of the seedpod of the lotus;
Fig. 2 is the brown stain degree broken line graph of test group and control group in the present embodiment, passing in time as can be seen from Figure, the brown stain degree of test group is always lower than control group, and variation tendency is slow, thus prove, after adopting preservation agent, can effectively prevent the brown stain of seedpod of the lotus epidermis;
Fig. 3 is the total phenol content broken line graph of test group and control group in the present embodiment, passing in time as can be seen from Figure, the total phenol content of test group is always higher than control group, and decline trend is slower than control group, thus prove, after adopting preservation agent, can effectively avoid the loss of benefit materials phenol;
Fig. 4 is the mda content broken line graph of test group and control group in the present embodiment, mda is the product of film ester peroxidation, its content increases the storage being unfavorable for fruit vegetables on the one hand, harmful on the other hand, consequently, it is desirable to its content is controlled a lower level, passing in time as can be seen from Figure, the mda content of test group is always lower than control group, and rising tendency is slow, and after thus proving to adopt preservation agent, the resistance of oxidation of the seedpod of the lotus strengthens;
Fig. 5 is the DPPH free radical scavenging activity broken line graph of test group and control group in the present embodiment, usually, along with the aging of fruit vegetables, in tissue, the equilibrium state of free radical is broken, cause the accumulation of excessive free radicals, and excessive free radical can cause the fracture of DNA with the DNA base generation addition reaction in tissue, its also can with the phosphatide generation peroxidation chain reaction in microbial film, thus destroy stability and the integrity of film, finally cause the necrosis of cell. DPPH free radical is often used to evaluation of tissue to total Scavenging activity of free radical. Passing in time as can be seen from Figure, the DPPH free radical scavenging activity of test group is always higher than control group, and variation tendency is slow, thus proves to adopt preservation agent process seedpod of the lotus tissue to have higher resistance of oxidation;
Fig. 6 is ultra-oxygen anion free radical (O2-) the clearance rate broken line graph of test group and control group in the present embodiment, and ultra-oxygen anion free radical is the free radical that in organism, first oxygen metabolism is formed, and is the predecessor of all oxyradicals. Under normal physiological conditions, the O2 in organism-constantly produce, is also constantly eliminated, and its concentration is in equilibrium state, and the O2 of physiological status-there is unique physiological function; But in fruit vegetables aging course, O2-Yin organizes the decline of resistance of oxidation and accumulates in body, thus cell tissue is produced injury, accelerate the aging of tissue. Therefore, ultra-oxygen anion free radical (O2-) clearance rate is also organize a kind of reflection of resistance of oxidation. Passing in time as can be seen from Figure, ultra-oxygen anion free radical (O2-) clearance rate of test group is always higher than control group, and variation tendency is slow, thus proves to adopt preservation agent process to maintain and adopts the rear seedpod of the lotus and organize higher resistance.
Fig. 7 is the active broken line graph of phenylalanine ammonia lyase (PAL) of test group and control group in the present embodiment, Fig. 8 is the active broken line graph of polyphenoloxidase (PPO) of test group and control group in the present embodiment, the active raising of phenylalanine ammonia lyase and polyphenoloxidase can reduce the content of the benefit materials phenylalanine in the seedpod of the lotus and polyphenol, it is thus desirable to by its activity control in lower level, passing in time as can be seen from Figure, the phenylalanine ammonia lyase (PAL) of test group and the active all lower than control group of polyphenoloxidase (PPO), and rising tendency is slow, after thus proving to adopt preservation agent, can by phenylalanine ammonia lyase in the seedpod of the lotus (PAL) and polyphenoloxidase (PPO) activity control a lower level, effectively preserve the freshness of the seedpod of the lotus,
Fig. 9 is the active broken line graph of catalase (CAT) of test group and control group in the present embodiment, Figure 10 is the active broken line graph of peroxidase (POD) of test group and control group in the present embodiment, Figure 11 is the active broken line graph of superoxide-dismutase (SOD) of test group and control group in the present embodiment, catalase (CAT), the active raising of peroxidase (POD) and superoxide-dismutase (SOD) can reduce the content of the objectionable impuritiess such as Peroxides and Superoxides, it is thus desirable to its activity to be remained on a higher level as far as possible, passing in time as can be seen from Figure, the catalase (CAT) of test group, the activity of peroxidase (POD) and superoxide-dismutase (SOD) is all higher than control group, after thus proving to adopt preservation agent, can by seedpod of the lotus hydrogen peroxide enzyme (CAT), the activity control of peroxidase (POD) and superoxide-dismutase (SOD) is a higher level, effectively reduce the generation of nuisance, ensure the freshness of the seedpod of the lotus.
Embodiment 2: a kind of preservation agent, comprises following component: lacquer tree fat 10g, Tefose2000 emulsifying agent 15g, hesperidine 98g, synephrine 12g, citric acid 29g, stearin 12g. Described hesperidine is Pericarpium Citri junoris extract, and concrete extracting method is homogenate extraction method: be that 65% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35 by orange peel dried powder and mass concentration, with flash extracter with 1.8 �� 105The VELOCITY EXTRACTION 4min of r/min, then through centrifugal, filters, and concentrated, recrystallization, obtains the Pericarpium Citri junoris extract that hesperidine purity is 90%; Described synephrine is Furctus Aurantii Immaturus extract, and concrete extracting method is homogenate extraction method: be that 60% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35 by dried immature fruit of citron orange dry fruit powder and mass concentration, with flash extracter with 1.8 �� 105The VELOCITY EXTRACTION 4min of r/min, then through centrifugal, filters, and concentrated, purifying, obtains the Furctus Aurantii Immaturus extract that synephrine purity is 55%.
The preparation method of above-mentioned preservation agent, comprises the following steps:
(1) 1 part of lacquer tree fat is added in 300g water, being heated to 50 DEG C, stir evenly in high-speed homogenization machine, stirring velocity is 5000r/min, it is warming up to 55 DEG C by the heat-up rate of 2 DEG C/min after churning time 5min, adds after stearin continues to stir 4min and be incubated;
(2) mix containing the Pericarpium Citri junoris extract of hesperidine and the Furctus Aurantii Immaturus extract containing synephrine, and stir evenly at 60 DEG C, then by the rate of addition of 3ml/min, mixed solution is added dropwise in the mixing liquid in step (1), 10min is stirred under 6000r/min, it is cooled to room temperature after finally adding Tefose2000 emulsifying agent and citric acid stirring 3min, obtains preservation agent.
Embodiment 3: a kind of preservation agent, comprises following component: lacquer tree fat 50g, Tefose2000 emulsifying agent 25g, hesperidine 52g, synephrine 89g, citric acid 11g, stearin 28g. Described hesperidine is Pericarpium Citri junoris extract, and concrete extracting method is homogenate extraction method: be that 70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:45 by orange peel dried powder and mass concentration, with flash extracter with 2.0 �� 105The VELOCITY EXTRACTION 10min of r/min, then through centrifugal, filters, and concentrated, recrystallization, obtains the Pericarpium Citri junoris extract that hesperidine purity is 93%; Described synephrine is Furctus Aurantii Immaturus extract, and concrete extracting method is homogenate extraction method: be that 70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:45 by dried immature fruit of citron orange dry fruit powder and mass concentration, with flash extracter with 2.0 �� 105The VELOCITY EXTRACTION 10min of r/min, then through centrifugal, filters, and concentrated, purifying, obtains the Furctus Aurantii Immaturus extract that synephrine purity is 63%.
The preparation method of above-mentioned preservation agent, comprises the following steps:
(1) 1 part of lacquer tree fat is added in 2500g water, being heated to 60 DEG C, stir evenly in high-speed homogenization machine, stirring velocity is 10000r/min, it is warming up to 70 DEG C by the heat-up rate of 5 DEG C/min after churning time 10min, adds after stearin continues to stir 6min and be incubated;
(2) mix containing the Pericarpium Citri junoris extract of hesperidine and the Furctus Aurantii Immaturus extract containing synephrine, and stir evenly at 70 DEG C, then by the rate of addition of 6ml/min, mixed solution is added dropwise in the mixing liquid in step (1), 15min is stirred under 10000r/min, it is cooled to room temperature after finally adding Tefose2000 emulsifying agent and citric acid stirring 6min, obtains preservation agent.
Preservation agent good refreshing effect prepared by present method, and all adopt pure natural raw material, harmless, it is possible not only to for the fresh-keeping of the seedpod of the lotus can also be used for the fresh-keeping of the vegetables and fruits such as apple, pear, tomato and grape.
Although as mentioned above, it is necessary, represented with reference to specific preferred embodiment and described the present invention, but it shall not be construed as the restriction to the present invention self. Under the spirit and scope of the present invention prerequisite not departing from claims definition, it can be made various change in the form and details.
Claims (8)
1. a preservation agent, it is characterised in that comprise following component, lacquer tree fat 10��50g, Tefose2000 emulsifying agent 15��25g, hesperidine 50��100g, synephrine 10��50g, citric acid 10��30g, stearin 10��30g.
2. preservation agent according to claim 1, it is characterised in that: described preservation agent comprises following component: lacquer tree fat 30g, Tefose2000 emulsifying agent 20g, hesperidine 85g, synephrine 36g, citric acid 16g, stearin 23g.
3. preservation agent according to claim 1, it is characterized in that: described hesperidine is Pericarpium Citri junoris extract, purity is 90%��95%, concrete extracting method is homogenate extraction method: be that 65%��70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35��45 by orange peel dried powder and mass concentration, with flash extracter with 1.8 �� 105R/min��2.0 �� 105VELOCITY EXTRACTION 4��the 10min of r/min, then through centrifugal, filters, and concentrated, recrystallization, obtains the Pericarpium Citri junoris extract that hesperidine purity is 90%��95%.
4. preservation agent according to claim 1, it is characterized in that: described synephrine is Furctus Aurantii Immaturus extract, purity is 55%��65%, concrete extracting method is homogenate extraction method: be that 60%��70% ethanolic soln is uniformly mixed by solid liquid volume ratio 1:35��45 by dried immature fruit of citron orange dry fruit powder and mass concentration, with flash extracter with 1.8 �� 105R/min��2.0 �� 105VELOCITY EXTRACTION 4��the 10min of r/min, then through centrifugal, filters, and concentrated, purifying, obtains the Furctus Aurantii Immaturus extract that synephrine purity is 55%��65%.
5. the preparation method of preservation agent according to any one of a Claims 1 to 4, it is characterised in that comprise the following steps:
(1) 1 part of lacquer tree fat is added in 30��50 parts of water, being heated to 50��60 DEG C, stir evenly in high-speed homogenization machine, stirring velocity is 5000��10000r/min, it is warming up to 55��70 DEG C after churning time 5��10min, adds after stearin continues stirring 4��8min and be incubated;
(2) mix containing the Pericarpium Citri junoris extract of hesperidine and the Furctus Aurantii Immaturus extract containing synephrine, and stir evenly at 60��70 DEG C, then mixed solution is added dropwise in the mixing liquid in step (1), 10��15min is stirred under 6000��10000r/min, it is cooled to room temperature after finally adding Tefose2000 emulsifying agent and citric acid stirring 3��6min, obtains preservation agent.
6. the preparation method of preservation agent according to claim 5, it is characterised in that: the speed heated up described in step (1) is 2��5 DEG C/min.
7. the preparation method of preservation agent according to claim 5, it is characterised in that: described in step (2), the rate of addition of mixed solution is 3��6ml/min.
8. the purposes of preservation agent according to any one of a Claims 1 to 4, it is characterised in that: can be used for the fresh-keeping of the seedpod of the lotus, apple, pear, tomato and grape.
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