CN105606603A - Method for determining content of formaldehyde in liquid - Google Patents
Method for determining content of formaldehyde in liquid Download PDFInfo
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- CN105606603A CN105606603A CN201511010712.2A CN201511010712A CN105606603A CN 105606603 A CN105606603 A CN 105606603A CN 201511010712 A CN201511010712 A CN 201511010712A CN 105606603 A CN105606603 A CN 105606603A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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Abstract
The invention discloses a method for determining the content of formaldehyde in liquid. The method comprises the following steps: taking 10g of liquid to be detected and centrifuging on a high-speed centrifuging machine to obtain supernatant; taking the supernatant and transferring the supernatant into an ultrafiltration cup; carrying out ultrafiltration by using an ultrafiltration membrane through an ultra-filter under a room temperature condition; taking filtered-out colorless clear liquid; preserving the liquid at -10 DEG C for measurement; adding a formaldehyde color developing agent into a clean ultraviolet cuvette; weighing 10g to 15g of sodium hydroxide and putting the sodium hydroxide into a volumetric flask; adding 20ml to 28ml of distilled water, and uniformly shaking; adding the mixture into the ultraviolet cuvette; taking 0.1mg of the colorless clear liquid and gradually adding the colorless clear liquid into the ultraviolet cuvette by using a trace sample feeding device; adding the sample and adding the formaldehyde color developing agent at the same time; standing for 20 to 30 minutes and detecting on an ultraviolet visible light spectrophotometer to judge a result. The method disclosed by the invention is rapid in developing reaction; determination conditions are relatively not strict; the method is high in sensitivity and low in cost, and has small interferences in a detection process; with regard to large-size and middle-size dairy enterprises or beverage enterprises, the method for rapidly determining the content of the formaldehyde in the liquid, disclosed by the invention, is very suitable for popularization.
Description
Technical field
The present invention relates to a kind of detection method, especially relate to a kind of method of measuring content of formaldehyde in liquid, belong to safety detection field.
Background technology
At present prohibit repeatly and do not stop in adulterated phenomenons such as the former milk of China, and more and more serious and general. Because formaldehyde has corrosion-proof and sterilization effect, under the ordering about of interests, some milk families add the microbiological indicator of formaldehyde when reaching milk collection in former milk, and who does not know that formaldehyde is for more highly toxic material, preferentially controls formaldehyde on list be in second at China's toxic chemical. Formaldehyde is defined as carcinogenic and causes deformed material by the World Health Organization, is generally acknowledged allergen, is also one of potential strong mutagen, and it can destroy biological cell albumen, can cause the diseases such as human allergy, enteron aisle IR, food poisoning. At present the fast detecting of formaldehyde in the liquid such as former milk is had to corresponding several detection method, but because being not easy to the not obvious or shortcoming that sensitivity is not high of safety operation, colour developing phenomenon, its quality can not be guaranteed.
Summary of the invention
The deficiency existing for prior art, technical problem to be solved by this invention is, a kind of method of measuring content of formaldehyde in liquid is provided, result accuracy and with a high credibility, easy to use, phenomenon is obvious, highly sensitive, and with low cost, handling safety.
For solving the problems of the technologies described above, the technical scheme that the present invention takes is that a kind of method of measuring content of formaldehyde in liquid, comprises the following steps:
(1) get 10g testing liquid centrifugal upper strata liquid that obtains on supercentrifuge;
(2) get above-mentioned upper strata liquid, proceed to ultrafiltration cup, use at ambient temperature milipore filter ultrafilter ultrafiltration, collect the colourless limpid liquid leaching, preserve at subzero 10 degree, for measurement;
(3) formaldehyde chromogenic reagent is added in clean ultraviolet cuvette;
(4) take 10-15g NaOH in volumetric flask, then add 20-28ml distilled water to shake up to add in above-mentioned ultraviolet cuvette;
(5) get the colourless limpid liquid of 0.1mg again, be added in ultraviolet cuvette gradually with microsyringe, application of sample limit, limit adds formaldehyde chromogenic reagent, after standing 20-30min, on UV, visible light spectrophotometer, detects and gets final product judged result.
Optimize, the method for content of formaldehyde in above-mentioned measurement liquid, the formaldehyde chromogenic reagent in its step (5) is phenol reagent.
Optimize, the method for content of formaldehyde in above-mentioned measurement liquid, the concentration of its phenol reagent is 0.015g ∕ ml.
Chromogenic reaction of the present invention is fast, only needs about one minute, and condition determination is relatively also loose, and highly sensitive, cost is low, disturbs also little in former milk detects, for big-and-middle-sized Dairy Enterprise or drink enterprise, it is a kind of method containing formaldehyde in universal fast detecting liquid that is highly suitable for.
Detailed description of the invention
Embodiment 1
The present invention is a kind of method of measuring content of formaldehyde in liquid, comprises the following steps: that (1) get 10g testing liquid centrifugal upper strata liquid on supercentrifuge;
(2) get above-mentioned upper strata liquid, proceed to ultrafiltration cup, use at ambient temperature milipore filter ultrafilter ultrafiltration, collect the colourless limpid liquid leaching, preserve at subzero 10 degree, for measurement;
(3) formaldehyde chromogenic reagent is added in clean ultraviolet cuvette;
(4) take 10g NaOH in volumetric flask, then add 20ml distilled water to shake up to add in above-mentioned ultraviolet cuvette;
(5) get the colourless limpid liquid of 0.1mg again, be added in ultraviolet cuvette gradually with microsyringe, application of sample limit, limit adds formaldehyde chromogenic reagent, after standing 20min, on UV, visible light spectrophotometer, detects and gets final product judged result. Formaldehyde chromogenic reagent in step (5) is phenol reagent; The concentration of phenol reagent is 0.015g ∕ ml.
Embodiment 2
The present invention is a kind of method of measuring content of formaldehyde in liquid, comprises the following steps: that (1) get 10g testing liquid centrifugal upper strata liquid on supercentrifuge;
(2) get above-mentioned upper strata liquid, proceed to ultrafiltration cup, use at ambient temperature milipore filter ultrafilter ultrafiltration, collect the colourless limpid liquid leaching, preserve at subzero 10 degree, for measurement;
(3) formaldehyde chromogenic reagent is added in clean ultraviolet cuvette;
(4) take 15g NaOH in volumetric flask, then add 28ml distilled water to shake up to add in above-mentioned ultraviolet cuvette;
(5) get the colourless limpid liquid of 0.1mg again, be added in ultraviolet cuvette gradually with microsyringe, application of sample limit, limit adds formaldehyde chromogenic reagent, after standing 30min, on UV, visible light spectrophotometer, detects and gets final product judged result. Formaldehyde chromogenic reagent in step (5) is phenol reagent; The concentration of phenol reagent is 0.015g ∕ ml.
Embodiment 3
The present invention is a kind of method of measuring content of formaldehyde in liquid, comprises the following steps: that (1) get 10g testing liquid centrifugal upper strata liquid on supercentrifuge;
(2) get above-mentioned upper strata liquid, proceed to ultrafiltration cup, use at ambient temperature milipore filter ultrafilter ultrafiltration, collect the colourless limpid liquid leaching, preserve at subzero 10 degree, for measurement;
(3) formaldehyde chromogenic reagent is added in clean ultraviolet cuvette;
(4) take 12g NaOH in volumetric flask, then add 24ml distilled water to shake up to add in above-mentioned ultraviolet cuvette;
(5) get the colourless limpid liquid of 0.1mg again, be added in ultraviolet cuvette gradually with microsyringe, application of sample limit, limit adds formaldehyde chromogenic reagent, after standing 25min, on UV, visible light spectrophotometer, detects and gets final product judged result. Formaldehyde chromogenic reagent in step (5) is phenol reagent; The concentration of phenol reagent is 0.015g ∕ ml. Chromogenic reaction of the present invention is fast, only needs about one minute, and condition determination is relatively also loose, and highly sensitive, cost is low, disturbs also little in former milk detects, for big-and-middle-sized Dairy Enterprise or drink enterprise, it is a kind of method containing formaldehyde in universal fast detecting liquid that is highly suitable for.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned giving an example; those skilled in the art; in essential scope of the present invention, variation, remodeling, interpolation or the replacement made, all should belong to protection scope of the present invention.
Claims (3)
1. a method of measuring content of formaldehyde in liquid, comprises the following steps:
(1) get 10g testing liquid centrifugal upper strata liquid that obtains on supercentrifuge;
(2) get above-mentioned upper strata liquid, proceed to ultrafiltration cup, use at ambient temperature milipore filter ultrafilter ultrafiltration, collect the colourless limpid liquid leaching, preserve at subzero 10 degree, for measurement;
(3) formaldehyde chromogenic reagent is added in clean ultraviolet cuvette;
(4) take 10-15g NaOH in volumetric flask, then add 20-28ml distilled water to shake up to add in above-mentioned ultraviolet cuvette;
(5) get the colourless limpid liquid of 0.1mg again, be added in ultraviolet cuvette gradually with microsyringe, application of sample limit, limit adds formaldehyde chromogenic reagent, after standing 20-30min, on UV, visible light spectrophotometer, detects and gets final product judged result.
2. the method for content of formaldehyde in measurement liquid according to claim 1, is characterized in that: the formaldehyde chromogenic reagent in described step (5) is phenol reagent.
3. the method for content of formaldehyde in measurement liquid according to claim 2, is characterized in that: the concentration of described phenol reagent is 0.015g ∕ ml.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010712.2A CN105606603A (en) | 2015-12-30 | 2015-12-30 | Method for determining content of formaldehyde in liquid |
| PCT/CN2016/112577 WO2017114406A1 (en) | 2015-12-30 | 2016-12-28 | Method for measuring formaldehyde content in liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511010712.2A CN105606603A (en) | 2015-12-30 | 2015-12-30 | Method for determining content of formaldehyde in liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105606603A true CN105606603A (en) | 2016-05-25 |
Family
ID=55986708
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511010712.2A Withdrawn CN105606603A (en) | 2015-12-30 | 2015-12-30 | Method for determining content of formaldehyde in liquid |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105606603A (en) |
| WO (1) | WO2017114406A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017114406A1 (en) * | 2015-12-30 | 2017-07-06 | 田琳琳 | Method for measuring formaldehyde content in liquid |
| CN109856068A (en) * | 2019-01-17 | 2019-06-07 | 浙江理工大学 | A kind of formaldehyde testing reagent and detection method based on Mannich reaction |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103076321B (en) * | 2012-11-26 | 2015-09-30 | 江苏中烟工业有限责任公司徐州卷烟厂 | A kind of Continuous Flow Analysis instrument detects the method for formaldehyde in smoke aqueous gel |
| CN103616380A (en) * | 2013-12-03 | 2014-03-05 | 广东中测食品化妆品安全评价中心有限公司 | Reagent and method for rapidly detecting formaldehyde in foods |
| CN104280389A (en) * | 2014-09-20 | 2015-01-14 | 中山鼎晟生物科技有限公司 | Detection method of formaldehyde in beverage |
| CN105606603A (en) * | 2015-12-30 | 2016-05-25 | 田琳琳 | Method for determining content of formaldehyde in liquid |
-
2015
- 2015-12-30 CN CN201511010712.2A patent/CN105606603A/en not_active Withdrawn
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2016
- 2016-12-28 WO PCT/CN2016/112577 patent/WO2017114406A1/en active Application Filing
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017114406A1 (en) * | 2015-12-30 | 2017-07-06 | 田琳琳 | Method for measuring formaldehyde content in liquid |
| CN109856068A (en) * | 2019-01-17 | 2019-06-07 | 浙江理工大学 | A kind of formaldehyde testing reagent and detection method based on Mannich reaction |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017114406A1 (en) | 2017-07-06 |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| WW01 | Invention patent application withdrawn after publication | ||
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Application publication date: 20160525 |