CN105603108A - 一种用于狗粪便污染检测的试剂盒及其制备与应用 - Google Patents
一种用于狗粪便污染检测的试剂盒及其制备与应用 Download PDFInfo
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- CN105603108A CN105603108A CN201610167403.4A CN201610167403A CN105603108A CN 105603108 A CN105603108 A CN 105603108A CN 201610167403 A CN201610167403 A CN 201610167403A CN 105603108 A CN105603108 A CN 105603108A
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- kit
- primer
- dog
- fecal pollution
- detecting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
正向引物( ED-2-F ) | ATTATTGGGCGTAAAGGGCG | SEQ ID NO.1 |
反向引物( ED-2-R ) | TCCTCTCCGATACTCTAGCAT | SEQ ID NO.2 |
正向引物(FUP-F) | CTAACTACGTGCCAGCAGCC | SEQ ID NO.3 |
反向引物(FUP-R) | GCCTTCGCCACTGGTGTTCC | SEQ ID NO.4 |
正向引物(ED-2F)(10uM/L) | 0.5ul |
反向引物(ED-2R)(10uM/L) | 0.5ul |
2×Power Taq PCR Master Mix | 7.5ul |
样品基因组DNA | 1ul |
无菌水补足至 | 15 ul |
正向引物(FUP-F)(10uM/L) | 0.5ul |
反向引物(FUP-R)(10uM/L) | 0.5ul |
2×Power Taq PCR Master Mix | 7.5ul |
样品基因组DNA | 1ul |
无菌水补足至 | 15 ul |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610167403.4A CN105603108B (zh) | 2016-03-22 | 2016-03-22 | 一种用于狗粪便污染检测的试剂盒及其制备与应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610167403.4A CN105603108B (zh) | 2016-03-22 | 2016-03-22 | 一种用于狗粪便污染检测的试剂盒及其制备与应用 |
Publications (2)
Publication Number | Publication Date |
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CN105603108A true CN105603108A (zh) | 2016-05-25 |
CN105603108B CN105603108B (zh) | 2019-03-15 |
Family
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CN201610167403.4A Active CN105603108B (zh) | 2016-03-22 | 2016-03-22 | 一种用于狗粪便污染检测的试剂盒及其制备与应用 |
Country Status (1)
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CN (1) | CN105603108B (zh) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104846113A (zh) * | 2015-06-05 | 2015-08-19 | 南京大学 | 一种同时检测水中狗、猪、鸡粪便污染的pcr试剂盒及其检测方法 |
US20150368695A1 (en) * | 2014-03-31 | 2015-12-24 | U.S. Environmental Protection Agency | Host-associated dna sequences, primers, and probes for pcr-based identification of dog fecal pollution sources |
-
2016
- 2016-03-22 CN CN201610167403.4A patent/CN105603108B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150368695A1 (en) * | 2014-03-31 | 2015-12-24 | U.S. Environmental Protection Agency | Host-associated dna sequences, primers, and probes for pcr-based identification of dog fecal pollution sources |
CN104846113A (zh) * | 2015-06-05 | 2015-08-19 | 南京大学 | 一种同时检测水中狗、猪、鸡粪便污染的pcr试剂盒及其检测方法 |
Non-Patent Citations (2)
Title |
---|
ZHENG G ET AL.: "Development of Faecalibacterium 16S rRNA gene marker for identification of human faeces", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
刘爱喜: "应用Faecalibacterium菌作为水体粪便污染指示菌的研究", 《中国优秀硕士学位论文全文数据库工程科技I辑》 * |
Also Published As
Publication number | Publication date |
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CN105603108B (zh) | 2019-03-15 |
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Effective date of registration: 20190606 Address after: 401332 6th Floor, Building 4, No. 99 Software Park Project, Xiyong Science and Technology Third Road, Shapingba District, Chongqing Patentee after: CHONGQING HUANRUI BIOTECHNOLOGY CO.,LTD. Address before: 400044 No. 174 Sha Jie street, Shapingba District, Chongqing Patentee before: Chongqing University |
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Effective date of registration: 20200119 Address after: 401329 standard workshop of Jinfeng biomedical industrial park, No.28, Gaoxin Avenue, Jiulongpo District, Chongqing Patentee after: Chongqing GAOJIN Biotechnology Co.,Ltd. Address before: 401332 6th Floor, Building 4, No. 99 Software Park Project, Xiyong Science and Technology Third Road, Shapingba District, Chongqing Patentee before: CHONGQING HUANRUI BIOTECHNOLOGY CO.,LTD. |
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Effective date of registration: 20230720 Address after: 7-2, No. 28 Nanqiao Fengjingyuan, Jiangbei District, Chongqing 400020 Patentee after: Chongqing Zhiyou Intellectual Property Service Co.,Ltd. Address before: 401329 standard workshop of Jinfeng biomedical industrial park, 28 Gaoxin Avenue, Jiulongpo District, Chongqing Patentee before: Chongqing GAOJIN Biotechnology Co.,Ltd. |