CN105603038A - Preparation method of high-dissolvability high-acyl gellan gum - Google Patents
Preparation method of high-dissolvability high-acyl gellan gum Download PDFInfo
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- CN105603038A CN105603038A CN201610088322.5A CN201610088322A CN105603038A CN 105603038 A CN105603038 A CN 105603038A CN 201610088322 A CN201610088322 A CN 201610088322A CN 105603038 A CN105603038 A CN 105603038A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Abstract
The invention discloses a preparation method of high-dissolvability high-acyl gellan gum and belongs to the field of high-acyl gellan gum. The preparation method comprises the steps that waterweed pseudomonas and bacillus pumilus are inoculated to a fermentation medium for fermentation, diatomite is added to a fermentation solution to serve as a filter aid, supernate is obtained through separation, ethyl alcohol is added to the supernate to generate flocculated gellan gum, the flocculated gellan gum is added to distilled water to be resolved, a sodium carbonate solution is added to generate a gellan gum precipitate, then the gellan gum is dissolved in water, and finally the high-dissolvability high-acyl gellan gum can be obtained through decoloration and spray drying. The prepared high-dissolvability high-acyl gellan gum is simple and novel in process, low in cost and high in yield, and the obtained product has good dissolvability, high purity and high transparency.
Description
Technical field
The invention discloses a kind of preparation method of high dissolving high acyl gellan gum, belong to high acyl gellan gum field.
Background technology
Gellan gum is a kind of line style polyanion microbial polysaccharide, has unique gel characteristic and solution rheoiogy character, and self-discovery rises and is applied in foods and cosmetics. In recent years, along with the development of biomedical subject, natural polymer gellan gum and hydrogel thereof, shown wide application prospect in the field such as drug delivery system and tissue engineering material. Gellan gum is nontoxic, has biocompatibility and biodegradable, the transparent and good stability of the hydrogel that forms, and the mechanical property of gel is close with human body normal tissue under certain condition.
High acyl gellan gum can be brought into play good gel, inborn nature, fixing and conjunctiva effect; high acyl gellan gum has following characteristic simultaneously: be a kind of gel reversible, that heat is reversible of shearing; and there is fabulous perfume bloom performance; can share with other hydrosol, have synergy, have splendid figuration effect with starch; be a kind of elastic gel, and can strengthen mouthfeel. Therefore, high acyl gellan gum can be widely used in medicine, chemical industry and food industry, is the favorable substitutes of carragheen, agar, pectin and other hydrophilic colloid, has wide market prospects.
The extracting method of domestic high acyl gellan gum is: first carries out salt precipitation, then carries out organic solvent washing, and drying and crushing, this technique can not be removed impurity etc. on colloid completely, and the high acyl gellan gum product purity making is low, poorly soluble.
Summary of the invention
The technical problem that the present invention mainly solves: poor for current existing high acyl gellan gum product appearance, purity is low, the problem that product gel strength is low and poorly soluble, a kind of preparation method of high dissolving high acyl gellan gum is provided, the present invention is inoculated into waterweed pseudomonad and bacillus pumilus in fermentation medium and ferments, to in zymotic fluid, add diatomite as filter aid, separate to obtain supernatant, in supernatant, add ethanol to generate flocculation gellan gum, flocculation gellan gum is added in distilled water and again dissolved, and add sodium carbonate liquor, generation gellan gum precipitation, again by water-soluble gellan gum dissolving, finally decolouring, spraying is dried and gets final product to obtain high dissolving high acyl gellan gum, the present invention prepares high simply novelty of technique of dissolving high acyl gellan gum, cost is low, yield is high, and the product of gained has good dissolubility and high-purity, the high grade of transparency.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) count by weight, get 30～40 portions of sucrose, 15～20 parts of peptones, 2～3 parts of sorghum flours, 18～22 parts of yeast extracts, 1.5～2.2 parts of dipotassium hydrogen phosphates, 0.5～1.8 part of magnesium sulfate and 21～23 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0;
(2) fermentation medium of getting after the above-mentioned pH of 1.0～1.5L has regulated is put into 3L fermentation tank, by inoculum concentration 8～10%, mixed bacteria liquid is put into fermentation tank, Temperature Setting is 30～35 DEG C, speed setting is 150～170r/min, ferment 8～10 days, described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes;
(3) after above-mentioned fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 0.8～1.5% diatomite, after stirring, leave standstill 30～40min, after leaving standstill, put into centrifuge, centrifugation 5～8min under 3000～5000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 30～35% in beaker, be uniformly mixed, there is floccule gellan gum to separate out;
(4) said mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 10～15% mass fraction 15% sodium carbonate liquors, have Precipitation, filter to such an extent that filter residue is gellan gum;
(5) gellan gum of above-mentioned gained being put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 6～8%, stir after 30～40min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
The method of application of the present invention: the high acyl gellan gum that the present invention can be made can be directly used in dairy products, in neutral dairy products, can be used alone 0.03～0.04% high acyl gellan gum as stabilizing agent, can reach suspension solid content, eliminate flocculation phenomenon texts, and there is thickening, the feature such as tasty and refreshing; In acidic dairy prod, can be by composite to 0.04～0.06% high acyl gellan gum and 0.2～0.4% sodium cellulose glycolate, then be used as stabilizing agent with calcium salt compatibility, high acyl gellan gum can play suspension solid content, thickening, stable effect.
The invention has the beneficial effects as follows:
(1) the present invention prepares high simply novelty of technique of dissolving high acyl gellan gum, and cost is low, and yield is high, does not produce waste water, to environment-friendly in preparation process;
(2) product that the present invention prepares has good dissolubility and high-purity, the high grade of transparency, in medicine, chemical industry and food industry, has a wide range of applications.
Detailed description of the invention
First meter by weight, get 30～40 portions of sucrose, 15～20 parts of peptones, 2～3 parts of sorghum flours, 18～22 parts of yeast extracts, 1.5～2.2 parts of dipotassium hydrogen phosphates, 0.5～1.8 part of magnesium sulfate and 21～23 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0; The fermentation medium of getting after the above-mentioned pH of 1.0～1.5L has regulated is put into 3L fermentation tank, by inoculum concentration 8～10%, mixed bacteria liquid is put into fermentation tank, Temperature Setting is 30～35 DEG C, speed setting is 150～170r/min, ferments 8～10 days, and described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes; After fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 0.8～1.5% diatomite, after stirring, leave standstill 30～40min, after leaving standstill, put into centrifuge, centrifugation 5～8min under 3000～5000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 30～35% in beaker, be uniformly mixed, there is floccule gellan gum to separate out; Mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 10～15% mass fraction 15% sodium carbonate liquors, have Precipitation, filter to such an extent that filter residue is gellan gum; The gellan gum of gained is put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 6～8%, stir after 30～40min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
Example 1
First meter by weight, get 30 portions of sucrose, 20 parts of peptones, 3 parts of sorghum flours, 20 parts of yeast extracts, 2.2 parts of dipotassium hydrogen phosphates, 1.8 parts of magnesium sulfate and 23 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0; The fermentation medium of getting after the above-mentioned pH of 1.5L has regulated is put into 3L fermentation tank, by inoculum concentration 10%, mixed bacteria liquid is put into fermentation tank, and Temperature Setting is 35 DEG C, and speed setting is 170r/min, ferments 10 days, and described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes; After fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 1.5% diatomite, after stirring, leave standstill 40min, after leaving standstill, put into centrifuge, centrifugation 8min under 5000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 35% in beaker, be uniformly mixed, there is floccule gellan gum to separate out; Mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 15% mass fraction 15% sodium carbonate liquor, have Precipitation, filter to such an extent that filter residue is gellan gum; The gellan gum of gained is put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 8%, stir after 40min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
The high acyl gellan gum that the present invention can be made can be directly used in dairy products, in neutral dairy products, can be used alone 0.03% high acyl gellan gum as stabilizing agent, can reach suspension solid content, eliminate flocculation phenomenon texts, and there is thickening, the feature such as tasty and refreshing; In acidic dairy prod, can be by composite to 0.04% high acyl gellan gum and 0.2% sodium cellulose glycolate, then be used as stabilizing agent with calcium salt compatibility, high acyl gellan gum can play suspension solid content, thickening, stable effect.
Example 2
First meter by weight, get 35 portions of sucrose, 15 parts of peptones, 2 parts of sorghum flours, 22 parts of yeast extracts, 1.5 parts of dipotassium hydrogen phosphates, 1.5 parts of magnesium sulfate and 23 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0; The fermentation medium of getting after the above-mentioned pH of 1.3L has regulated is put into 3L fermentation tank, by inoculum concentration 9%, mixed bacteria liquid is put into fermentation tank, and Temperature Setting is 33 DEG C, and speed setting is 160r/min, ferments 9 days, and described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes; After fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 1.2% diatomite, after stirring, leave standstill 35min, after leaving standstill, put into centrifuge, centrifugation 6min under 4000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 33% in beaker, be uniformly mixed, there is floccule gellan gum to separate out; Mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 13% mass fraction 15% sodium carbonate liquor, have Precipitation, filter to such an extent that filter residue is gellan gum; The gellan gum of gained is put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 7%, stir after 35min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
The high acyl gellan gum that the present invention can be made can be directly used in dairy products, in neutral dairy products, can be used alone 0.035% high acyl gellan gum as stabilizing agent, can reach suspension solid content, eliminate flocculation phenomenon texts, and there is thickening, the feature such as tasty and refreshing; In acidic dairy prod, can be by composite to 0.05% high acyl gellan gum and 0.3% sodium cellulose glycolate, then be used as stabilizing agent with calcium salt compatibility, high acyl gellan gum can play suspension solid content, thickening, stable effect.
Example 3
First meter by weight, get 40 portions of sucrose, 15 parts of peptones, 2 parts of sorghum flours, 18 parts of yeast extracts, 2 parts of dipotassium hydrogen phosphates, 1 part of magnesium sulfate and 22 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0; The fermentation medium of getting after the above-mentioned pH of 1.5L has regulated is put into 3L fermentation tank, by inoculum concentration 10%, mixed bacteria liquid is put into fermentation tank, and Temperature Setting is 35 DEG C, and speed setting is 170r/min, ferments 10 days, and described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes; After fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 1.5% diatomite, after stirring, leave standstill 40min, after leaving standstill, put into centrifuge, centrifugation 8min under 5000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 35% in beaker, be uniformly mixed, there is floccule gellan gum to separate out; Mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 15% mass fraction 15% sodium carbonate liquor, have Precipitation, filter to such an extent that filter residue is gellan gum; The gellan gum of gained is put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 8%, stir after 40min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
The high acyl gellan gum that the present invention can be made can be directly used in dairy products, in neutral dairy products, can be used alone 0.04% high acyl gellan gum as stabilizing agent, can reach suspension solid content, eliminate flocculation phenomenon texts, and there is thickening, the feature such as tasty and refreshing; In acidic dairy prod, can be by composite to 0.06% high acyl gellan gum and 0.4% sodium cellulose glycolate, then be used as stabilizing agent with calcium salt compatibility, high acyl gellan gum can play suspension solid content, thickening, stable effect.
Claims (1)
1. a preparation method for high dissolving high acyl gellan gum, is characterized in that concrete preparation process is
(1) count by weight, get 30～40 portions of sucrose, 15～20 parts of peptones, 2～3 parts of sorghum flours, 18～22 parts of yeast extracts, 1.5～2.2 parts of dipotassium hydrogen phosphates, 0.5～1.8 part of magnesium sulfate and 21～23 parts of distilled water, after mixing, mixture is placed on to sterilizing under uviol lamp, obtain fermentation medium, the ammoniacal liquor that service property (quality) mark is 2% regulates fermentation medium pH to 7.0;
(2) fermentation medium of getting after the above-mentioned pH of 1.0～1.5L has regulated is put into 3L fermentation tank, by inoculum concentration 8～10%, mixed bacteria liquid is put into fermentation tank, Temperature Setting is 30～35 DEG C, speed setting is 150～170r/min, ferment 8～10 days, described mixed bacteria liquid is that concentration is 1.5 × 108Waterweed pseudomonad bacterium liquid and the concentration of individual/mL are 0.6 × 108The bacillus pumilus liquid 1:1 by volume of individual/mL, mixes;
(3) after above-mentioned fermentation ends, collect zymotic fluid, add wherein zymotic fluid quality 0.8～1.5% diatomite, after stirring, leave standstill 30～40min, after leaving standstill, put into centrifuge, centrifugation 5～8min under 3000～5000r/min, collects supernatant, the supernatant of gained is put into beaker, to the absolute ethyl alcohol that adds supernatant volume 30～35% in beaker, be uniformly mixed, there is floccule gellan gum to separate out;
(4) said mixture is filtered with cellulose acetate filter membrane, remove waste liquid, obtain flocculate gellan gum, flocculation gellan gum is joined in distilled water to dispersing and dissolving again by solid-liquid mass ratio 1:5, obtain gellan gum liquid, add again gellan gum liquid volume 10～15% mass fraction 15% sodium carbonate liquors, have Precipitation, filter to such an extent that filter residue is gellan gum;
(5) gellan gum of above-mentioned gained being put into spray dryer sprays dry; collect dry thing and put into beaker; add wherein the water of 70 DEG C; stir until dry thing stops adding water after dissolving completely; add wherein again 200 order carclazytes of dry matter amount 6～8%, stir after 30～40min filtration under diminished pressure while hot, collect filtrate and put into spray dryer and spray and be dried; collect dry thing, can obtain high dispersive low-acyl gellan gum.
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| CN201610088322.5A CN105603038A (en) | 2016-02-17 | 2016-02-17 | Preparation method of high-dissolvability high-acyl gellan gum |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
| CN111777383A (en) * | 2020-07-17 | 2020-10-16 | 中铁建工集团有限公司 | Self-curing steel pipe concrete and preparation method thereof |
| CN113274953A (en) * | 2020-12-01 | 2021-08-20 | 上海时宜品牌管理有限公司 | Formaldehyde removal gel and preparation method thereof |
-
2016
- 2016-02-17 CN CN201610088322.5A patent/CN105603038A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108690143A (en) * | 2017-04-07 | 2018-10-23 | 帝斯曼知识产权资产管理有限公司 | A kind of production method of high transparency low-acyl gellan gum |
| CN111777383A (en) * | 2020-07-17 | 2020-10-16 | 中铁建工集团有限公司 | Self-curing steel pipe concrete and preparation method thereof |
| CN111777383B (en) * | 2020-07-17 | 2022-03-25 | 中铁建工集团有限公司 | Self-curing steel pipe concrete and preparation method thereof |
| CN113274953A (en) * | 2020-12-01 | 2021-08-20 | 上海时宜品牌管理有限公司 | Formaldehyde removal gel and preparation method thereof |
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