CN105602835A - Application of preservation box in cold storage of biological material and method thereof - Google Patents
Application of preservation box in cold storage of biological material and method thereof Download PDFInfo
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- CN105602835A CN105602835A CN201610075807.0A CN201610075807A CN105602835A CN 105602835 A CN105602835 A CN 105602835A CN 201610075807 A CN201610075807 A CN 201610075807A CN 105602835 A CN105602835 A CN 105602835A
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- crisper
- refrigeration
- cold storage
- main body
- preservation box
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- Microbiology (AREA)
- Biotechnology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses an application of a preservation box in cold storage of a biological material and a method thereof. An ordinary household plastic preservation box serving as a tool is used for cold storage of a biological material so as to realize a purpose of preventing the contamination and drying the biological material. The method comprises the following steps: 1) taking out a clean preservation box, and inversely arranging a box cover onto a desktop; 2) placing the biological material to be cold-stored on the preservation box cover, taking a preservation box main body to inversely cover the material, and locking the box cover to form an inverted preservation box; 3) placing the material into a cold storage chamber; 4) using after the cold storage. The application and the method have the characteristics and advantages that: 1) the operation is simple and convenient; the effect for preventing the contamination and drying the material is good, and the long-term cold storage requirement can be met; 2) the method is particularly suitable for storing a panel culture material already producing a great amount of spores so as to meet the requirement on artificial inoculation or other researches utilizing the spores; 3) the preservation boxes can be stacked, so that the effective storage space in the cold storage chamber of a refrigerator can be sufficiently utilized; and moreover, due to the protection of the preservation box, the collision and falling accident of culture dishes can be effectively reduced.
Description
Technical field
The present invention relates to that plant pathology learns a skill and microbiological technique. Specifically a kind of crisper is at biological materialApplication and method thereof in material refrigeration.
Background technology
In the routine work of Plant Pathology/microbiology area research, often need to carry out the life of microorganismLong hair is educated research, as bacterium colony and mycelium morphology observation, the Study on influencing factors of biological growth; Control medicineBiologicall test research; The cultivation preparation research of pathogen brood body spore, comprises and produces spore characteristic research, sporeThe influence condition research that son forms etc. The mode that these researchs of growing are normally cultivated by flat board is carried outWork, in the course of the work, often need to stop growth of microorganism developmental process temporarily or will growThe thalline material of developmental condition is lived and is preserved when state is fixed with length. Realize these targets, conventionally adopt at presentThe method of getting is that (K cryogenic treatment) processed in refrigeration, because K cryogenic treatment is the most convenient effective technical measures, and shouldTechnical measures can block growing of organism, can keep again the activity of organism. Because flat board is cultivatedUtensil be mainly culture dish, thereby refrigeration process method practice on often, by pathogen/microorganismMaterial proceeds in general refrigerator and places together with culture medium and culture dish. Due to the indoor temperature of refrigerator cold-storageGenerally constant not, add frequent switch gate, cause temp. in refrigerating chamber fluctuation larger, thereby cause cultivatingWare inner air and outer air turnover exchange is frequent, is easy to cause biomaterial in culture dish to pollute; And refrigerator is coldHide the environment that chamber belongs to low humidity, this low wet environment is easy to cause organism and cultivation base material in culture dishDryization of material, from and cause biomorph structural change and bioinactivation. Current overcome above-mentioned technology lackSunken method, is mainly to adopt Parafilm sealed membrane by the method for culture dish opening part sealing label, adopts the partyAlthough method can make up above-mentioned technological deficiency to a certain extent, the method has other technology disadvantage,The Parafilm sealed membrane that current exactly laboratory is used is the film product of lengthy motion picture rolling, when use, needs elder generationBe cut into the strip of sheet that is applicable to stickup, the sealed membrane of then getting this strip of sheet draws one by one at culture dish outer surfaceStretch stickup, operate quite loaded down with trivial detailsly, operating efficiency is not high, and in the time refrigerating in batches, practical operation is very inconvenient; AndAnd in the refrigerating chamber of refrigerator, often easily there is moisture regain moisture film in culture dish surface, sticks on culture dishParafilm sealed membrane easily produces and gets loose and cracking phenomena, prevention biomaterial pollute and dry effect stillUndesirable.
Content of the present invention
The object of this invention is to provide application and the method thereof of a kind of crisper in biomaterial refrigeration.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
Application and the method thereof of crisper in biomaterial refrigeration, adopts normal domestic use plastic preserving boxAs instrument, refrigeration biomaterial, reach prevent biomaterial pollute and dry object, operating procedure asUnder:
1. crisper is prepared: take out clean sealing plastic crisper, open fresh-keeping box cover, and lid is anti-Come over, in lid, face up and be placed on desktop.
2. material is inserted to crisper: take out the culture dish that is loaded with biomaterial that needs refrigeration from culturing room,Be put in step 1) on the fresh-keeping box cover got ready, go bail for fresh box main body conversely, cover on culture dish, andThe opening periphery of box main body is snapped in the seal groove of lid, the clasp clinching of lid is prominent in the button of box main body,Fresh-keeping box cover is connected fastening with box main body, in crisper, in sealing state, form the inner biology that loadsThe inversion crisper of material.
3. material is inserted to refrigerating chamber: by step 2) the inversion crisper of end of operation proceeds to the refrigeration of refrigeratorIndoor storage.
4. after refrigeration, using: by step 3) crisper of refrigeration takes out, and is placed on desktop, breaks lid into two with one's handsClasp, takes away box main body, and biomaterial the normal operating of taking out refrigeration are used.
Characteristic of the present invention and advantage:
1) simple and convenient; Effect pre-anti-pollution and that material is dry is high.
2) due to the good airproof performance of crisper, culture medium is not easy to be dried, and is well suited for the need of long-time refrigerationWant.
3) be particularly suitable for the dull and stereotyped culture materials of large volume production spore, available artificial infection or other use of storageThe research of spore needs.
4) the stackable placement of crisper, can make full use of the indoor effective parking space of refrigerator cold-storage, andAnd due to the protection of crisper, can effectively reduce knocking over of culture dish and fall and fall accident.
Brief description of the drawings
Fig. 1 is the plastic preserving box outside drawing that the present invention uses; In figure, a is complete crisper, and b protectsFresh box main body, c is the outside of fresh-keeping box cover, and d is the inner face of fresh-keeping box cover, and e is the clasp on lid, fBe that button on box main body is prominent, g is seal groove and the fluid sealant on lid.
Fig. 2 adopts the performance of the dull and stereotyped cultivation of the inventive method refrigeration bacterium colony after 6 months; In figure, a cultivatesBase flat board, b is bacterium colony.
Fig. 3 adopts the performance after 3 months at the dull and stereotyped cultivation of refrigeration bacterium colony of conventional refrigeration method. In figure, a isCulture dish, b is that culture medium flat plate and bacterium colony are the state that drying shrinkage is rolled.
Fig. 4 is that crisper is inverted use schematic diagram, and in figure, a is the state of culture dish holding on lid; B isCulture dish is covered in the state in box by box main body; C is the positive configuration state of crisper.
Detailed description of the invention:
Inventor, in the routine work of false smut and rice blast research, often needs to preserve large volume production sporeFlat-plate bacterial colony, current store method is mainly to take low temperature memory method, but easily goes out after deepfreezeExisting living contaminants and dryization of material, produce larger interference to research work. Owing to adopting sealed membrane methodImprovement effect also undesirable, inventor finds separately and explores new method, chooses a kind of family expenses on the marketPlastic preserving box carries out culture materials cold storage test, finds that it prevents from polluting with dry effect superior. This guarantorFresh box is made up of box main body and lid, and by clasp and the prominent tight joint of button, as shown in Figure 1.
Adopt the flat-plate bacterial colony material of this crisper loading ustilaginoidea virens (or Pyricularia oryzae), through 6 monthsRefrigeration after, the state of the material in culture dish before still can Keep cool, as shown in Figure 2, but meanwhileAdopt the dull and stereotyped culture materials of conventional method refrigeration, only spend 3 months, the material in culture dish has become dryState, as shown in Figure 3, demonstrates method satisfactory for result that refrigerates dull and stereotyped culture materials with crisper.
Tracing it to its cause, is to have a fluid sealant on the lid of this crisper, after crisper closing lid, and the inside and outside tool of boxStandby airproof effect, by this crisper packaged flat plate culture materials, can make culture materials remain on officeThe environment that portion is clean, also can stop the moisture loss of culture materials, the crisper that this sealing property is good,Although family expenses are general at present, also do not find the technical performance that it is good and be used in laboratory.
The daily family expenses of crisper are normally just being put use, the box main body of crisper under, lid is upper,As shown in a of Fig. 1, but laboratory just putting while use, and the operation of the taking-up of culture dish/put into box main body is very tiredDifficulty, for solving this technical problem, is inverted crisper to use, first the lid of crisper is placed on desktop,In lid, face up, as shown in the d of Fig. 1, culture dish is seated on this lid, then it is anti-to get box main bodyCome over, culture dish covered in box main body, as shown in Figure 4, like this, culture dish to get/put operation very squareJust.
Although the inventive method operates simple and easy, storage biomaterial prevention material contamination and dry aspectEffect quite desirable. Culture dish is inserted in crisper on superclean bench by sterile working method, thenCarry out material chilled, the better effects if of preventing living contaminants.
Embodiment 1
Adopt application and the method thereof of a kind of crisper in biomaterial refrigeration, refrigeration Pyricularia oryzae bacterium colony andConidium, as follows operation:
1. crisper is prepared
Take out clean sealing plastic crisper, open fresh-keeping box cover, by lid conversely, the inner face of lidBe placed in upward on desktop.
2. material is inserted to crisper
Take out and produced a large amount of conidial Pyricularia oryzae flat-plate bacterial colonies from culturing room, relay in step 1On the fresh-keeping box cover of getting ready, go bail for fresh box main body instead conversely, cover on culture dish, and by box main bodyOpening periphery snaps in the seal groove of lid, and the clasp clinching of lid is prominent in the button of box main body, makes fresh-keepingLid connects fastening with box main body, in crisper, in sealing state, form the inner Pyricularia oryzae bacterium colony that loadsWith conidial inversion crisper.
3. material is inserted to refrigerating chamber
The inversion crisper of step 2 end of operation is proceeded to the refrigeration indoor storage of refrigerator.
4. after refrigeration, use
The crisper that step 3 was refrigerated after 2 months takes out, and is inverted on desktop, breaks the clasp of lid into two with one's hands,Take away box main body, Pyricularia oryzae bacterium colony and the conidium of taking out refrigeration are used, and it is cold that flat-plate bacterial colony keeps substantiallyState before Tibetan, the conidium on bacterium colony more than 90% keeps normal activity, and keeps without living contaminantsState.
And the flat-plate bacterial colony that the employing conventional refrigeration method of processing is in contrast preserved has become dry, on bacterium colonyConidium is inactivation, and occurs living contaminants.
Embodiment 2
Adopt application and the method thereof of a kind of crisper in biomaterial refrigeration, refrigeration ustilaginoidea virens spore,Operate by embodiment 1 step 1 to step 4, but as different from Example 1, step 2 is got from culturing roomWhat go out is ustilaginoidea virens flat-plate bacterial colony; The time that step 4 refrigerates is 6 months.
The state of ustilaginoidea virens flat-plate bacterial colony after refrigeration before Keep cool substantially, as shown in Figure 2, still can washBrush out a large amount of thin-walled conidiums for. And contrast CK adopts the bacterium colony of conventional method refrigeration, 3 monthsRear bacterium colony drying shrinkage is rolled, as shown in Figure 3, cannot normally scrub out thin-walled conidium for.
Claims (1)
1. application and the method thereof of crisper in biomaterial refrigeration, is characterized in that, adopts commonDomestic plastic crisper is as instrument, and refrigeration biomaterial, reaches and prevent that biomaterial from polluting and dry order, operating procedure is as follows:
1) crisper is prepared: take out clean sealing plastic crisper, open fresh-keeping box cover, and lid is anti-Come over, in lid, face up and be placed on desktop;
2) material is inserted to crisper: take out the culture dish that is loaded with biomaterial that needs refrigeration from culturing room,Be put in step 1) on the fresh-keeping box cover got ready, go bail for fresh box main body conversely, cover on culture dish, andThe opening periphery of box main body is snapped in the seal groove of lid, the clasp clinching of lid is prominent in the button of box main body,Fresh-keeping box cover is connected fastening with box main body, in crisper, in sealing state, form the inner biology that loadsThe inversion crisper of material;
3) material is inserted to refrigerating chamber: by step 2) the inversion crisper of end of operation proceeds to the refrigeration of refrigeratorIndoor storage;
4) after refrigeration, using: by step 3) crisper of refrigeration takes out, and is placed on desktop, breaks lid into two with one's handsClasp, takes away box main body, and biomaterial the normal operating of taking out refrigeration are used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075807.0A CN105602835A (en) | 2016-02-03 | 2016-02-03 | Application of preservation box in cold storage of biological material and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610075807.0A CN105602835A (en) | 2016-02-03 | 2016-02-03 | Application of preservation box in cold storage of biological material and method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105602835A true CN105602835A (en) | 2016-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610075807.0A Pending CN105602835A (en) | 2016-02-03 | 2016-02-03 | Application of preservation box in cold storage of biological material and method thereof |
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| Country | Link |
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| CN (1) | CN105602835A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120510A (en) * | 2010-01-07 | 2011-07-13 | 方毅 | Double-cover buckled-type plastic preservation box |
| CN204021508U (en) * | 2014-04-22 | 2014-12-17 | 宁波市鄞州古林波晶塑料五金厂 | Plastic preserving box |
-
2016
- 2016-02-03 CN CN201610075807.0A patent/CN105602835A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102120510A (en) * | 2010-01-07 | 2011-07-13 | 方毅 | Double-cover buckled-type plastic preservation box |
| CN204021508U (en) * | 2014-04-22 | 2014-12-17 | 宁波市鄞州古林波晶塑料五金厂 | Plastic preserving box |
Non-Patent Citations (3)
| Title |
|---|
| 吴梦珂等: "食品储物保鲜盒的改良设计研究", 《科技创新导报》 * |
| 极品囝仔: "《百度百科》", 24 January 2016 * |
| 陈敏: "乐扣乐扣：小容器里创新大生意", 《中关村》 * |
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Application publication date: 20160525 |
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